3 results about "Avian infectious bronchitis" patented technology

The application discloses a kind of avian influenza and avian infectious bronchitis two-union recombinant subunit vaccine and its preparation method and application.The recombinant protein is by replacing the head domain of AIV HA protein with the RBD domain of IBV S protein, while adding GCN4, Trimer-tag trimer tag in sequence, the recombinant protein obtained, the recombinant protein is expressed in vitro using insect cells and prepared into subunit vaccine.The vaccine prepared by the application can produce high-level neutralizing antibodies against homologous and heterologous H9N2 subtype avian influenza virus after immunizing chicken population, while significantly reducing the virus discharge level and the histopathological changes caused, and can also induce high-level specific antibodies and neutralizing antibodies against QX subtype avian infectious bronchitis virus, effectively inhibit discharge, viral load, and reduce tissue lesions.The application does not depend on chicken embryo, and has low production cost and short cycle, and can simultaneously prevent avian influenza and avian infectious bronchitis.

The application discloses a compound traditional Chinese medicine, a preparation method and application thereof in treating chicken infectious bronchitis, and aims at the pathogenic characteristics of chicken infectious bronchitis, combines traditional Chinese medicine theory and prescription rules, and prepares a compound traditional Chinese medicine which can treat chicken infectious bronchitis, and the compound traditional Chinese medicine is prepared from the following raw materials: Radix Stemonae, Platycodonis, Glycyrrhizae, Eriobotryae and Rehmanniae. The compound traditional Chinese medicine has the effects of relieving cough and reducing sputum, clearing heat and resolving toxin, and benefiting water and removing dampness through scientific compounding of monarchs, ministers, aids and messengers. The main highlight of the application is to improve the immune response level of the body, and the chicken infectious bronchitis virus is prevented from further invading the lung, and the treatment effect is good. The compound traditional Chinese medicine has obvious treatment effect on chicken infectious bronchitis, the method is simple and easy to implement, is green and safe, and has important significance for reducing the use of antiviral chemicals in breeding production.

This invention relates to the field of biomedical technology, specifically to the application of forsythoside in the preparation of drugs for the prevention and treatment of infectious bronchitis in chickens. Immunofluorescence and Western blotting results show that forsythoside can significantly inhibit the expression of viral nucleocapsid proteins in CEK cells and reduce the viral load in CEK cells. Furthermore, forsythoside can protect against IBV infection in broiler chickens. Quantitative fluorescence results indicate a significant reduction in viral replication in the tracheal tissue of broilers. Clinical symptoms and histopathological results show that forsythoside can significantly alleviate IBV infection-induced slow weight gain, respiratory distress, and tracheal tissue damage in broilers. This invention demonstrates that forsythoside has low cytotoxicity, can effectively prevent infectious bronchitis in chickens, and has advantages such as being green and safe, leaving no residue, having few toxic side effects, and being unlikely to induce drug resistance.