211 results about "Avian infectious bronchitis" patented technology

The invention discloses a infectivity bronchitis attenuated vaccine strain LDT3-A strain, and discloses application and application effect thereof in preventing and curing chicken infectivity bronchitis. The microorganism accession number of the attenuated vaccine strain is CGMCC-2902. The attenuated vaccine strain of the present invention has good safety and good immunization protection effect to the chicken infectivity bronchitis. The attenuated strain can be prepared into single vaccine or combined vaccine for preventing or curing infectivity bronchitis virus.

The method relates to an attenuated vaccine strain for avian infectious bronchitis virus and application thereof. According to the invention, avian infectious bronchitis virus (IBV) is attenuated, so as to successfully obtain an IBV attenuated strain CCTCC.V201232 and a derivative virus strain thereof. The attenuated strain and derivative virus strain provided by the invention can be used in preparation of a vaccine composition for prevention of infectious bronchitis. Experiments show that the attenuated strain and vaccine composition provided by the invention can be inoculated to immature birds, so as to effectively activate immune system in the birds and well prevent avian infectious bronchitis.

The invention provides a traditional Chinese medicine composition for preventing and treating avian infectious bronchitis and a preparation method thereof; the traditional Chinese medicine composition comprises the following components by weight: 2-4 parts of honeysuckle, 2-4 parts of cordate houttuynia, 1-2 parts of radix scrophulariae, 1-2 parts of isatis root, 2-4 parts of folium isatidis, 1-2 parts of platycodon grandiflorum, 1-2 parts of licorice, 1-2 parts of bitter almond, 1-2 parts of ephedra sinica, and 1-2 parts of gypsum; the traditional Chinese medicine granule is prepared by decoction and auxiliary material addition; the traditional Chinese medicine composition is reasonably formulated by traditional Chinese medicine herbs, provides an effective treatment medicament for preventing and treating avian infectious bronchitis for poultry industry, has the characteristics of quick effect, definite curative effect, no medicinal residue, and no any toxic or side effect; the preparation method is simple, and is applicable to requirements for large-scale industrial production.

The invention relates to a newcastle disease virus / avian influenza virus H9 subtype / infectious bronchitis virus triplex fluorescence quantification detection reagent and a detection method and belongs to the technical field of animal quarantine. A newcastle disease virus M gene coding region specific sequence, an avian influenza virus H9 subtype H gene coding region specific sequence and a chicken infectious bronchitis virus M gene coding region specific sequence are selected as target regions, and on the basis of multi-sequence comparison, primer and probe design is conducted. The length of primers is about 20 basic groups, the GC content is 50-60%, a two-stage structure and repeatability do not exist in the primers, no complementary sequence exists between the primers or in the primers, and the melting temperature (Tm value) difference between the primers is smaller than 5 DEG C. In order to guarantee universal use of a newcastle disease virus probe, the length of the probe is only 13 basic groups, the probe is modified by LAN, and the Tm value of the probe is increased. The lengths of the other two virus probes are both about 25 basic groups, and the Tm values are about 5 DEG C higher than those of the primers.

The invention discloses a chicken new castle disease-infectious bronchitis trivalent combined vaccine and a preparation method thereof. The trivalent combined vaccine comprises antigens and a freeze-drying protective agent. The antigens comprise a chicken kidney-type infectious bronchitis virus K136 strain having an accession number of CCTCC-V201320, a chicken breath-type infectious bronchitis virus H120 strain, and a chicken new castle disease virus La Sota strain. A mode of inoculating the K136 strain and the La Sota strain in a homeomorphism manner and inoculating the H120 strain individually is adopted for preparing the antigens. Based on the mode, after culture products are mixed according to a ratio to prepare the antigens, a product of the vaccine is obtained by adding the freeze-drying protective agent. The trivalent combined vaccine can prevent the chicken new castle disease and the chicken infectious bronchitis simultaneously, thus solving effectively a problem that vaccines at present can only provide a part of protection or cannot provide protection for the kidney-type IB, and achieving an objective that one injection can be used for preventing two diseases.

The invention discloses an animal epidemic disease three-color fluorescence RT-PCR detection kit and a detection method thereof, and is characterized in that the kit comprises multiple fluorescence RT-PCR reaction mother liquor, a positive control, a negative control, an AMV reverse transcriptase, and a Taq polymerase, wherein the multiple fluorescence RT-PCR reaction mother liquor contains a multiple 10*fluorescence RT-PCR reaction buffer, an avian influenza primer and a probe, a newcastle disease virus primer and a probe, an avian infectious bronchitis primer and a probe, and bovine serum albumin, wherein sequences of the forward primers, reverse primers, and specific probes are as shown in SEQIDNO. 1, NO. 2, NO.3, NO.4, NO.5, NO.6, NO.7. NO.8, NO.9, and NO.10. The advantages of the invention are that avian influenza, newcastle disease, and avian infectious bronchitis viruses can be detected simultaneously in a same reaction tube; the specificity is strong; the sensitivity is high, is rapid and accurate.

The invention discloses a triple egg yolk antibody against newcastle disease virus, avian influenza virus and infectious bronchitis virus and a preparation method and application thereof. The preparation method of the egg yolk antibody comprises the following steps of: (1) selecting a hen; (2) performing triple inactivation immunization of the hen obtained by the step (1) by use of newcastle disease virus, infectious bronchitis virus and avian influenza virus; (3) taking the egg laid by the hen and separating the egg yolk liquid; and (4) separating and purifying an egg yolk antibody from the egg yolk liquid obtained by the step (3). The triple egg yolk antibody disclosed by the invention is of great value in treating and preventing newcastle disease, avian influenza and infectious bronchitis.

The invention belongs to the immunity application field, which relates to the related fields of animal molecular biochemistry and the immunology, etc. The invention discloses a reagent kit respectively used for antibody of infectious bronchitis of chicken, which comprises a case body, and the test paper card and sample diluent equipped inside the case body, wherein, the test paper card is formed by the absorption pad, nitrocellulose membrane, gold-label pad and sample pad affixed in turn on the non-absorbent supporting flake by taking the sample pad, absorption pad and the NP recombinant proteins coated with the nucleocapsid of the infectious bronchitis of chicken as the detection line and the NP recombinant proteins coated with anti-mouse IgG as nitrocellulose membrane of the quality control line. The reagent kit used for testing the corresponding antibody has the obvious advantages of strong specificity, high sensitivity, easy operation and fast diagnosis.

The present invention discloses one kind of recombinant protein antigen for chicken infectious bronchitis virus (IBV) S1 and its preparation process and use. These nucleotide sequences are shown in SEQ ID No. 1~3. The present invention provides expression method of pronucleus encoding 3 truncated S1 proteins, can express the amino end 288 bp and 309 bp part segments of S1 protein of three IBV strains separately, and provides the purification method of the S1 protein expressing products of the three IBV strains. During detection with IBV positive serum, the expressed partial S1 protein has excellent antigen reaction, so that the present invention may be used as diagnosis antigen used in different detection methods for detecting IBV infection or immunologically produced antibody. In addition, the expressed partial S1 protein may be sued in preparing monoclonal antibody and polyclonal antibody for detecting IBV antigen and preparing subunit vaccine.

The invention discloses an efficacy test method of infectious bronchitis inactivated vaccines, which can be applied to vaccine quality control specifically comprising challenge protection rate control and egg-laying efficacy control. Experiments prove that the invention uses the method for detecting the HI antibody titer to replace the animal challenge test, and the method has the characteristics of simpleness, convenience, rapidness, accurate results, strong repeatability, strong specificity and the like and has the significance of popularization.

The invention belongs to the field of infectious disease detection of animals, and concretely relates to a triple rapid detection kit of three avian respiratory diseases, and an application thereof. The kit comprises a test paper strip, a test card shell and a sample dilution, and the test paper strip is formed by sequentially pasting an absorbing pad, a cellulose nitrate membrane, a colloidal-gold pad and a sample pad on a non-absorbent support slice; a combination pad is coated with an anti-avian influenza virus monoclonal antibody-colloidal gold marker, an anti-Newcastle disease virus monoclonal antibody-colloidal gold marker and an anti-chicken infectious bronchitis virus monoclonal antibody-colloidal gold marker mixture, detection lines T1, T2 and T3 are coated with the anti-avian influenza monoclonal antibody, the anti-Newcastle disease virus monoclonal antibody and the chicken infectious bronchitis monoclonal antibody, and the cellulose nitrate membrane is coated with a rabbit anti-rat IgG quality control line. The kit can simultaneously complete the disease cause detection of bird flu H5 type, H9 subtype, Newcastle diseases and chicken infectious bronchitis, and has strong specificity and high sensitivity.

The invention provides a preparation method of a bivalent vaccine by taking newcastle disease virus La Sota strain and infectious bronchitis virus N-S multi-epitope protein as antigen. To immunize 21-day-old chickens, the bivalent vaccine prepared by the method disclosed by the invention can be used for simultaneously preventing newcastle disease and nephrotropic infectious bronchitis virus infection, and the antibody level of the bivalent vaccine is higher than that of respectively immunizing by single vaccine, so that immunological stress is effectively relieved and immunity time is reduced.

A fowl-kidney-form infectious bronchitis HN99 virus has excellent immunogenicity. The diameter of coronal virus particle is 90-105 nm, it has membrane and is radiant with 18 nm vomer protrusion, it has no interference for NDV La Sota virus multiplying and no agglutination for 1% fowl red blood cell; regression flow experiment leads to respiratory tract symptom and renal boss. Ill fowl virus isolate has positive reaction with HN99 positive blood serum, has negative reaction with NDV La Sota strain, IBDV B87 strain and AIV H9N2 strains; HN99 positive blood serum has positive reaction with T strains, Hubei strain, Tianjin strain and Shandong strain and has negative reaction with other viruses. It has exogenous pathogenic pollution.

The invention relates to a medicinal composition for treating avian infectious bronchitis. The medicinal composition is characterized by comprising the following medicaments in part by weight: 1 to 2 parts of honeysuckle flower, 1 to 2 parts of indigowoad root, 1 to 2 parts of weeping forsythia, 0.5 to 1 part of dandelion, 0.5 to 1 part of Chinese violet, 0.5 to 1 part of platycodon root, 0.5 to 1 part of blackberry lily rhizome and 0.4 to 0.6 part of raw liquoric root. The preparation method comprises the following steps of: weighing the eight medicaments serving as raw materials according to the weight ratio; adding water in an amount which is 4 times the amount of the medicaments; decocting twice, 1.5 hours every time; combining the decoction; filtering; standing the filtrate for 12 hours; concentrating supernatant; adding ethanol until alcohol content is 60 percent; and placing, filtering, adding water, filtering, filling and sealing and circulating steam at the temperature of 100 DEG C for 30 minutes. The medicinal composition has the advantage that the medicinal composition is a pure Chinese medicinal preparation, so that the medicinal composition does not have any residual and toxic or side effects on a body. The medicinal composition can effectively prevent infectious laryngotracheitis of chicken.

The invention discloses a medicine for preventing and curing the infectious bronchitis of chicken (IBV). The medicine is prepared by mixing the following Chinese herbal medicines in certain proportion: radix isatidis, pyrrosia leaf, double blossom, radix scutellariae, bulbus fritillariae cirrhosae, apricot, common clubmoss herb, ephedra, angelica dahurica, raw white alum, fructus aristolochiae, gypsum, rhizoma anemarrhenae, forsythia, fried radish seed, fried pepperweed seed, fried perilla-seed, mongolian snakegourd root, inula britannica chinensis, rhubarb, gardenia and raw liquorice. The materials are dried, separated from impurities, crushed, milled, mixed and then evenly stirred. During the clinical use, the powder is immersed in boiled hot water for 30 to 60 minutes, so that the effective drug ingredients are fully dissolved and separated out. The medicine is helpful for the internal absorption of the sick chicken. Meanwhile, the addition of a little bit of sugar and VC into the medicine liquid can improve the effect of the medicine. The medicine has high cure rate clinically, no adverse reaction, no hidden risk of drug residue and drug resistance. The invention has simple preparation and convenient usage and can be widely used in the chicken raising industry.

The invention discloses a preparation method of a chicken infectious bronchitis virus HA antigen. In the preparation method, an A-type clostridium perfringen is adopted to treat a virus solution, so that the problem that in the conventional method, the preparation cost of the antigen is high is solved; an adopted stabilizing agent is N-2-hydroxypropyl trimethyl ammonia chloride chitosan and is water-soluble, so that the problem that the conventional stabilizing agent chitosan is water-insoluble is solved; an adopted deactivating method is deactivation for 30 minutes in water bath of 56 DEG C, so that the problem that the titer is reduced after a deactivating agent is added is solved; an adopted chicken red blood cell suspension has the concentration of 0.5 percent, so that the judgment result is more stable and the problem that the judgment result is not accurate is solved; due to adoption of dialysis bag for concentration, the problem that the titer of the antigen is low is solved; therefore, the invention provides the preparation method of the chicken infectious bronchitis virus HA antigen, which is low in cost, high in titer, and high in stability and safety, and the prepared IBV (infectious bronchitis virus) HA antigen can be successfully applied to detection on the titer of a serum HI antibody after chickens are immunized by an IBV vaccine.

The invention discloses a method for preparing a chicken infectious bronchitis virus HA antigen. The chicken infectious bronchitis virus HA antigen is prepared by the steps of virus multiplication, virus solution concentration and phospholipase C treatment, and antigen inactivation and stabilization. The stability of the prepared HA antigen at a temperature of 4 DEG C is prolonged to more than 6 months from the original 24 hours; besides, the valence of antigen is stable so as to solve the difficult problems which are not solved for a long time that the antigen is temporarily prepared for the test of each time and needs to be detected before use, and reduce the workload of the HI test. The prepared IBV HA antigen can be successfully applied to detecting the valence of antibody of serum HI after the chicken IBV vaccine immunization, and solves the problem that the detection of the IBV vaccine immunization effect is realized through neutralization tests in China for a long time, thereby wasting time and energy and having the danger of poison diffusion.

The invention discloses a medicine for treating infectious bronchitis of chicken. The medicine is prepared by the following raw materials: white mulberry root-bark, rhizoma anemarrhenae, bitter apricot seed, radix peucedani, honeysuckle, fructus forsythiae, platycodon root, liquorice, reddish orange and baical skullcap root, and can be manufactured into various medicament forms. Practice statistical data shows that the medicine has smaller side effects on poultry while achieving favorable cure rate and is subject to no remarkable undesirable reaction.

The invention relates to a method for producing a newcastle disease, infectious bronchitis, egg drop syndrome and avian influenza (H9 subtype) combined inactivated vaccine. A newcastle disease virus La Sota strain, a gallinaceous infectious bronchitis virus M41 strain, an egg drop syndrome virus NE4 strain and an A-type avian influenza virus (H9 subtype) YBF003 strain serve as production virus strains, and are mixed with a high-quality adjuvant to be prepared into the inactivated vaccine through a super concentration process; after an animal is immunized by the vaccine, an antibody is rapidly produced, the titer is high, the protection period is long, and occurrence and spread of diseases are reduced.

The invention relates to a traditional Chinese medicine composition for treating chicken infectious bronchitis. The composition is prepared from the following medical raw materials: 20-40g of honeysuckle, 20-40g of fructus forsythiae, 8-12g of reed rhizome, 8-12g of fineleaf schizonepeta herb, 8-12g of mint, 8-12g of burdock, 8-12g of fermented soybean, 8-12g of bamboo leaf, 8-12g of platycodon grandiflorum, 8-12g of radix bupleuri, 8-12g of artemisia apiacea, 8-12g of bulbus fritilariae, 8-12g of white gourd seed, 8-12g of coix seed and 8-12g of peach seed. The invention has the effects of antiviral property, anti-anaphylaxis, antibiosis, inflammation diminishing, cough relief and sputum reduction and has an effective rate of 98 percent to the chicken infectious bronchitis. The inventionalso has safety, high efficiency and quick curative effect and cannot generate drug resistance.

The invention provides a detection method of a traditional Chinese medicine preparation for preventing infectious bronchitis. The traditional Chinese medicine preparation is prepared from roots of Baikal skullcap, fructus forsythia, cape jasmine fruits, honeysuckle, pogostemon cablin, Chinese thorowax roots and Thunberg fritillary bulb. The quality detection method includes identification and content determination projects. The thin-layer chromatography identification method for seven traditional Chinese medicines is clear in spot, good in repeatability, negative and free from interference, and can serve as a traditional Chinese medicine composition quality control method. A built baicalein content determination method is high in precision and accurate in measuring result, and product quality can be effectively controlled, so that clinical curative effects are ensured.

The invention relates to a live vaccine for infectious chicken bronchitis. The live vaccine consists of an antigen and a vaccine adjuvant, wherein the antigen comprises infectious chicken bronchitis viral strain with a collection code CGMCC No. 6752. The prepared vaccine can be used to prevent glandular stomach-type infectious chicken bronchitis. Furthermore, the infectious chicken bronchitis virus constituting the vaccine is a low virulent strain obtained by passage several times, thus reducing the side effect caused by the existing infectious bronchitis virus as the antigen.

The invention relates to the technical field of biological detection, and aims at providing a kit for RT-PCR typing detection of an avian infectious bronchitis virus. The kit comprises the following three pairs of primers including the universal primers designed according to an S1 gene segment of the infectious bronchitis virus, the 4 / 91 type virulent strain specific primers and the kidney type virulent strain specific primers designed according to an nsp12 gene segment, and the sequences of the three pairs of the primers are shown in SEQ ID NO:6-11. According to the kit, the purpose of performing typing detection on the IBV can be achieved only through one time of an RT-PCR reaction, and identification detection on the IBV virulent strains of the kidney type, the 4 / 91 type and the respiration type through the one-time RT-PCR method is achieved for the first time in the industry; the advantages of being convenient and rapid to use, high in sensibility and specificity and the like are achieved, and a novel effective detection means is supplied to clinical rapid identification diagnosis of the IBV, epidemiological investigation and IBV prevention and control.

The invention relates to liquorice and gallbladder oral liquid mainly treating lung heat cough caused by avian infectious bronchitis and mycoplasma gallisepticum infection, oral, injection and spray preparations containing a liquorice and gallbladder composite and a preparation method and a quality detection method thereof. The liquorice and gallbladder oral liquid comprises the components of isatis root, calculus bovis factitius, liquorice, borneol, porcine gall powder, compound of glauber-salt and liquorice and the like. The invention method has simple and convenient production process and advanced detection means; the product has stable quality, high bioavailability, no toxic and side effects, convenient use and obvious curative effect and has the effects of clearing heat and detoxicating, cooling blood and releasing the lung and relieving cough and asthma.

The invention discloses an LDL-T strain as an attenuated vaccine strain for avian infectious bronchitis and application thereof, which belong to the field of prevention and treatment of the avian infectious bronchitis. The attenuated live vaccine strain CGMCC No.12549 for the avian infectious bronchitis provided by the invention has good safety and immune protection, the immune protection rate reaches 80 percent or more, and the LDL-T strain has good protective efficacy on TW I type avian infectious bronchitis viruses, and can effectively protect chickens against the attack of the TW I type infectious bronchitis viruses with strong virulent; moreover, the LDL-T strain is safe for chickens and laying breeders, side reaction does not exist, and the safety is good. The cold-adapted attenuated vaccine strain disclosed by the invention can be applied to the preparation of single vaccines, mixed vaccines and the like for preventing and treating the avian infectious bronchitis viruses.

The invention relates to an artificially weakened infectious bronchitis virus and application thereof. The artificially weakened infectious bronchitis virus uses the infectious bronchitis virus (IBV) which has good immunogenicity and is separated by an inventor to be passaged and attenuated, so as to obtain an attenuated strain FNO-E55 as a virus seed for production to prepare a safe and effective infectious bronchitis live vaccine. The strain used in the invention can effectively prevent occurrence of infectious bronchitis resulted from the infectious bronchitis virus (793 / B serotype) by aiming at a prevalence situation of the current domestic infectious bronchitis.

The invention discloses an application of avian infectious bronchitis virus vaccine strain in the preparation of inactivated vaccine. The separated wild virulent strain of infectious bronchitis virus is subjected to chick embryo adaptability domestication so as to obtain an infectious bronchitis virus vaccine strain, which can well adapt to the chick embryo, maintains good hereditary characters and immunogenicity, and is named as ck / CH / LLN101135. Then the obtained infectious bronchitis virus vaccine strain is inactivated and subjected to immune efficacy evaluation experiments. The experiment results show that the infectious bronchitis inactivated vaccine prepared from the vaccine strain can trigger the organism to generate a good immune response, can prominently improve the motive immune protective effectiveness of chicken at the same time, and effectively protects chicken from being affected by infectious bronchitis virus. The infectious bronchitis virus vaccine strain can be used to prepare single vaccine or combined vaccine of avian infectious bronchitis.

The invention discloses an S1 and N double-antigen protein recombinant plasmid capable of expressing IBV (Infectious Bronchitis Virus) and a construction method and an application thereof. The inventor designs primers according to S1, N and Ub gene sequences of infectious bronchitis virus registered in GenBank, amplifies the S1 and N genes of China isolate GX-YL5 strain of the infectious bronchitis virus and a chicken Ub gene through PCR (Polymerase Chain Reaction), inserts the N and S1 genes of the China isolate GX-YL5 strain of the infectious bronchitis virus and the chicken Ub gene into an eukaryotic expression vector pVAX1 through a molecular biological method, thus constructing an expression plasmid capable of co-expressing the N and S1 genes and the chicken Ub gene and researches DNA vaccine Pvax1-Ub-linker-N-S1. Experiments prove that the vaccine has a significant curative effect of preventing IBV. The S1 and N double-antigen protein recombinant plasmid disclosed by the invention can be used for simultaneously inserting the S1 and N genes of IBV and the chicken Ub gene which are connected in series into the pVAX1 vector to prevent the infectious bronchitis virus.

An infectious bronchitis virus low virulent strain, a constructing method thereof and applications of the strain are disclosed. An infectious bronchitis virus high virulent strain of the QX-Like genotype, which is separated in the field, is adopted as a female parent, and is subjected to 100 or above generations of continuous passage through chicken embryos to obtain the attenuated virulent strain. The microbial preservation accession number of the low virulent strain is CGMCC No.13292. The S1 sequence of genome of the low virulent strain is shown as SEQ ID No.1. Chickens immunized with the low virulent strain do not die, and are free of obvious tissue organ lesions and clinical symptoms, thus proving that the low virulent strain is safe to chickens and good in safety. The challenge protection rate of the low virulent strain for immunized chickens is 80-100% so that the low virulent strain has good immunogenicity. Virulence enhancement tests prove that virulence of the low virulent strain is not enhanced, and the low virulent strain is stable in heredity and is suitable as a vaccine strain or a virus seed for preventing and treating infectious bronchitis.

The invention discloses a traditional Chinese medicine feed for preventing and treating infectious bronchitis and a preparation method thereof. The traditional Chinese medicine feed for preventing and treating infectious bronchitis is composed of the following active components in certain parts by weight: wheat bran, corn, bean pulp, biotin, yeast powder, calcium carbonate, chromium pyridinyl formate, amino acid chelated manganese, table salt and traditional Chinese medicine effective components. According to the traditional Chinese medicine feed for preventing and treating infectious bronchitis, multiple Chinese herbal medicines, multiplex vitamins and the like are added into the conventional feed raw materials, so the traditional Chinese medicine feed contains multiplex vitamins, microelements and Chinese herbal medicine components, and is capable of enhancing the immunologic function of chickens and enhancing the disease resistance of organisms. The Chinese herbal medicines are derived from nature, and thus, the traditional Chinese medicine feed has the advantages of abound resources, low price, small toxic and side effects, no residue, wide applicability and the like. The traditional Chinese medicine feed for preventing and treating infectious bronchitis has wide prospects in promoting animal growth and preventing and treating infectious bronchitis.