11 results about "Avian infectious bronchitis" patented technology

This invention discloses a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, its preparation method, and its application, belonging to the field of traditional Chinese medicine composition technology. The raw materials of this traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, by mass percentage, include 8%-12% extract of honeysuckle branches and leaves, 8%-12% extract of dipsacus root, 8%-12% chrysanthemum, 4%-6% mulberry leaves, 4%-6% licorice root, 0.8%-1.2% peppermint, and 50.8%-67.2% anhydrous glucose. It has good anti-inflammatory effects and can effectively alleviate inflammatory responses caused by LPS. In particular, it has good therapeutic and ameliorative effects on the clinically prevalent porcine reproductive and respiratory syndrome (PRRS) and infectious bronchitis in chickens, effectively reducing morbidity and mortality, and improving production performance.

The invention relates to an infectious bronchitis subunit vaccine based on a nano-skeleton technology. Specifically, the invention provides a fusion protein which sequentially comprises an infectious bronchitis virus S protein fragment or a mutant thereof, a connecting peptide and helicobacter pylori ferritin from an N terminal to a C terminal, wherein the infectious bronchitis virus S protein fragment is S1 protein, and the amino acid sequence of the S protein fragment is as shown in SEQ ID NO. 2; the mutant of the chicken infectious bronchitis virus S protein fragment is S1-2 protein or S1-3 protein, the amino acid sequence of the S1-2 protein is shown as SEQ ID NO.4, and the amino acid sequence of the S1-3 protein is shown as SEQ ID NO.5. The invention further discloses a preparation method of the chicken infectious bronchitis virus S protein fragment.

The application discloses a kind of avian influenza and avian infectious bronchitis two-union recombinant subunit vaccine and its preparation method and application.The recombinant protein is by replacing the head domain of AIV HA protein with the RBD domain of IBV S protein, while adding GCN4, Trimer-tag trimer tag in sequence, the recombinant protein obtained, the recombinant protein is expressed in vitro using insect cells and prepared into subunit vaccine.The vaccine prepared by the application can produce high-level neutralizing antibodies against homologous and heterologous H9N2 subtype avian influenza virus after immunizing chicken population, while significantly reducing the virus discharge level and the histopathological changes caused, and can also induce high-level specific antibodies and neutralizing antibodies against QX subtype avian infectious bronchitis virus, effectively inhibit discharge, viral load, and reduce tissue lesions.The application does not depend on chicken embryo, and has low production cost and short cycle, and can simultaneously prevent avian influenza and avian infectious bronchitis.

The invention discloses an avian influenza and avian infectious bronchitis bigeminy recombinant subunit vaccine as well as a preparation method and application thereof. The recombinant protein is obtained by replacing a head structural domain of an AIV HA protein with an RBD structural domain of an IBV S protein and adding GCN4 and Trimer-tag trimer tags into a sequence, and the recombinant protein is expressed in vitro by using insect cells and is prepared into a subunit vaccine. After chicken flocks are immunized by the prepared vaccine, high-level neutralizing antibodies aiming at homologous and heterologous H9N2 subtype avian influenza viruses can be generated, meanwhile, the detoxification level of the viruses and caused tissue pathological changes are remarkably reduced, and high-level specific antibodies and neutralizing antibodies aiming at QX subtype avian infectious bronchitis viruses can also be induced; toxin expelling and virus load are effectively inhibited, and tissue lesion is relieved. The method does not depend on chick embryos, is low in production cost and short in period, and can prevent avian influenza and avian infectious bronchitis at the same time.

The invention belongs to the field of veterinary drugs, and relates to an infectious bronchitis nanoparticle antigen and a vaccine thereof. The avian infectious bronchitis nanoparticle antigen is a helicobacter pylori ferritin nanoparticle with an avian infectious bronchitis virus S1 protein fragment. The nanoparticle vaccine comprises an immunizing dose of the avian infectious bronchitis nanoparticle antigen and a pharmaceutically acceptable carrier. The vaccine has the excellent effects of being high in safety, good in immunogenicity, stable in batches and capable of providing complete protection for attacking the infectious bronchitis virus.

The application discloses a compound traditional Chinese medicine, a preparation method and application thereof in treating chicken infectious bronchitis, and aims at the pathogenic characteristics of chicken infectious bronchitis, combines traditional Chinese medicine theory and prescription rules, and prepares a compound traditional Chinese medicine which can treat chicken infectious bronchitis, and the compound traditional Chinese medicine is prepared from the following raw materials: Radix Stemonae, Platycodonis, Glycyrrhizae, Eriobotryae and Rehmanniae. The compound traditional Chinese medicine has the effects of relieving cough and reducing sputum, clearing heat and resolving toxin, and benefiting water and removing dampness through scientific compounding of monarchs, ministers, aids and messengers. The main highlight of the application is to improve the immune response level of the body, and the chicken infectious bronchitis virus is prevented from further invading the lung, and the treatment effect is good. The compound traditional Chinese medicine has obvious treatment effect on chicken infectious bronchitis, the method is simple and easy to implement, is green and safe, and has important significance for reducing the use of antiviral chemicals in breeding production.

The invention discloses a chicken infectious bronchitis prediction method based on an AI chip, and relates to the technical field of animal epidemic disease intelligent monitoring and prediction, and the method comprises the following steps: S001, building a nanosecond-level unified time reference, continuously collecting chicken house environment sound signals through a distributed acoustic collection unit, generating a phase and energy joint spectrum according to the time reference, and carrying out the detection of the phase and energy joint spectrum; and stripping fundamental tone and harmonic components of low-frequency mechanical vibration by using a spectral difference operator to form a dynamic reference signal with separated sound sources. According to the method, the acoustic processing link is driven through the nanosecond time base, dynamic extraction and reconstruction of cough sound are achieved, a false alarm path is recognized in combination with residual density and confidence gating, an interference field is constructed through inverse sound wave injection, the recognition threshold value is dynamically adjusted, and the prediction precision and stability of an AI chip in a high-noise environment are improved.

The invention discloses an IBV (Infectious Bronchitis Virus) N (Infectious Bronchitis Virus)-protein-targeted natural medicine for resisting avian infectious bronchitis virus as well as a screening method and application thereof. The unique active ingredient of the natural medicine is salvianolic acid B (the CAS number is 115939-25-8, and the purity is greater than or equal to 99.92%), and the natural medicine can be specifically combined with a 10 * 10 * 10 active pocket (coordinates X are equal to 12.3, Y is equal to 8.7 and Z is equal to 10.2) of IBV M41 strain N protein, so that virus replication is remarkably inhibited. The screening method adopts a three-stage process of computer virtual screening, chick embryo in-egg verification and in-vivo and in-vitro confirmation, and drug activity and safety are ensured through layer-by-layer screening of molecular docking, chick embryo experiments, cell experiments and chick in-vivo experiments. The medicine is high in safety (the survival rate of CEK cells is larger than or equal to 92.5%, and no obvious toxicity exists), remarkable in antiviral effect (the chicken virus load is reduced by 70%-82%, and the clinical symptom remission rate is larger than or equal to 50%), capable of being orally taken (10-20 mg / kg body weight, one treatment course of 7 days) and various in dosage form (powder, oral liquid and granules), is used for preparing the avian infectious bronchitis resisting medicine, fills the market blank and has important application value.

The invention discloses a compound traditional Chinese medicine aqueous extract for preventing and treating infectious bronchitis as well as a preparation method and application thereof, and belongs to the field of veterinary traditional Chinese medicine preparations. The aqueous extract is prepared from rhizoma pinellinae praeparata, bulbus fritillariae thunbergii, pericarpium citri reticulatae, liquorice, platycodon grandiflorum and semen armeniacae amarae according to a specific proportion through water extraction and concentration. Aiming at the defects of an existing prevention and treatment scheme in recovery of the growth performance and immune function of the infected chicken, the invention innovatively adopts a treatment rule formula of combining qi regulating, spleen strengthening, dampness eliminating, phlegm reducing and lung qi diffusing and lowering. Animal experiments show that the compound can effectively inhibit avian infectious bronchitis virus replication, can significantly improve the thymus index of infected chicks, and enables the average daily gain of the chicks to recover to a level that the average daily gain is not significantly different from that of a healthy group, and the synergistic effect is significantly higher than that of a ribavirin positive control group. The composition is simple in component, stable in process and good in safety, and a new product is provided for green and efficient prevention and treatment of infectious bronchitis.

The invention discloses a SOCS1 antagonistic peptide for preventing and treating avian infectious bronchitis and application of the SOCS1 antagonistic peptide, the amino acid sequence of the SOCS1 antagonistic peptide is LPQDKEYYKVKEP, and Y is phosphorylated tyrosine. Reasonable design is carried out on the basis of a molecular mechanism of interaction of a JAK2 activation ring and the SOCS1 protein, a molecular docking result shows that the SOCS1 antagonistic peptide can be precisely combined to KIR and SH2 functional domains of the chicken SOCS1 protein through a specific phosphorylated tyrosine residue of the SOCS1 antagonistic peptide, and the combination can competitively block combination of SOCS1 and endogenous JAK2 of cells, so that the chicken SOCS1 antagonistic peptide can be used for preparing the chicken SOCS1 antagonistic peptide. Therefore, the inhibition effect of SOCS1 on a JAK-STAT signal channel is relieved, the I-type interferon antiviral immune response of a host is recovered, and virus replication is inhibited fundamentally.

This invention relates to the field of biomedical technology, specifically to the application of forsythoside in the preparation of drugs for the prevention and treatment of infectious bronchitis in chickens. Immunofluorescence and Western blotting results show that forsythoside can significantly inhibit the expression of viral nucleocapsid proteins in CEK cells and reduce the viral load in CEK cells. Furthermore, forsythoside can protect against IBV infection in broiler chickens. Quantitative fluorescence results indicate a significant reduction in viral replication in the tracheal tissue of broilers. Clinical symptoms and histopathological results show that forsythoside can significantly alleviate IBV infection-induced slow weight gain, respiratory distress, and tracheal tissue damage in broilers. This invention demonstrates that forsythoside has low cytotoxicity, can effectively prevent infectious bronchitis in chickens, and has advantages such as being green and safe, leaving no residue, having few toxic side effects, and being unlikely to induce drug resistance.