61 results about "Infectious bronchitis virus" patented technology

The invention belongs to the field of veterinary drugs, and relates to an avian infectious bronchitis virus antigen recombinant protein S-Trimer and a subunit vaccine thereof. The invention provides an avian infectious bronchitis virus antigen recombinant protein S-Trimer. The amino acid sequence of the antigen recombinant protein S-Trimer is as shown in SEQ ID No. 4. The invention provides an infectious bronchitis virus antigen. The infectious bronchitis virus antigen is of a trimer structure of the recombinant protein S-Trimer. The invention provides a chicken infectious bronchitis virus subunit vaccine. The subunit vaccine comprises a pharmaceutically acceptable carrier and an immune dose of the chicken infectious bronchitis virus antigen. The vaccine is high in safety, good in immunogenicity and stable in batches, and can provide complete protection for attacking the infectious bronchitis virus.

The invention belongs to the technical field of biology, and particularly relates to a primer probe composition and a kit for simultaneously detecting IBV (Infectious Bursal Virus) and six genotypes thereof. The primer probe composition is used for detecting the avian infectious bronchitis virus of the GI-1 genotype, the avian infectious bronchitis virus of the GI-13 genotype, the avian infectious bronchitis virus of the GI-19 genotype and the avian infectious bronchitis virus of the GI-22 genotype. The kit is used for detecting the avian infectious bronchitis virus of GVI-1 genotype, detecting the avian infectious bronchitis virus of GI-7 genotype or detecting the avian infectious bronchitis virus of general genotype. The method is high in detection flux, can finish typing and general detection of six genotypes in a single-tube reaction system, and is time-saving and efficient; the channel utilization rate is high, and each fluorescent channel supports typing of two genotypes, so that multiple capabilities are effectively expanded; the typing accuracy is high, typing signals are clear, and the accuracy rate is high.

The invention provides an infectious bronchitis virus subunit vaccine as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering vaccines. According to the chicken infectious bronchitis virus mutant protein disclosed by the invention, the amino acid sequence of the mutant protein is shown as SEQ ID NO.5. According to the avian infectious bronchitis virus subunit vaccine containing the recombinant avian infectious bronchitis virus mutant protein Kozak-IgKsp-IBV-M41-S-core-6His as an effective component, the glycosylation level of the vaccine is relatively high, the expression level is relatively stable, the virus return risk of a live vaccine does not exist, the safety is high, large-scale industrial preparation can be achieved, and the vaccine has good application prospects. The cost is low.

The present invention belongs to the field of biotechnology, and specifically relates to a primer-probe combination and a kit for simultaneously detecting IBV and its six genotypes. The primer-probe combination is for detecting GI-1 genotype of avian infectious bronchitis virus, for detecting GI-13 genotype of avian infectious bronchitis virus, for detecting GI-19 genotype of avian infectious bronchitis virus, for detecting GI-22 genotype of avian infectious bronchitis virus, for detecting GVI-1 genotype of avian infectious bronchitis virus, for detecting GI-7 genotype of avian infectious bronchitis virus or for detecting universal genotype of avian infectious bronchitis virus. The detection throughput of the present invention is high, and typing and universal detection of six genotypes can be completed in a single-tube reaction system, which is time-saving and efficient; the channel utilization rate is high, and each fluorescent channel supports typing of two genotypes, effectively expanding the multiplexing capability; the typing accuracy is strong, the typing signal is clear, and the accuracy rate is high.

The present application belongs to the field of veterinary drugs, and provides a recombinant protein S-Trimer of an infectious bronchitis virus antigen and a subunit vaccine therefor. Provided in the present application is a recombinant protein S-Trimer of an infectious bronchitis virus antigen, wherein the recombinant protein S-Trimer of the antigen has an amino acid sequence as set forth in SEQ ID No. 4. Also provided in the present application is an infectious bronchitis virus antigen. The infectious bronchitis virus antigen is of a trimer structure of the recombinant protein S-Trimer described above. The present application provides a subunit vaccine for an infectious bronchitis virus, wherein the subunit vaccine comprises a pharmaceutically acceptable carrier and an immunogenic amount of the infectious bronchitis virus antigen.

The invention provides a Chinese herbal medicine preparation for treating avian infectious bronchitis virus variant infection, and relates to the technical field of medicine preparation. The Chinese herbal medicine preparation for treating avian infectious bronchitis virus variant infection is prepared from the following components in parts by weight: 12-20 parts of gynostemma pentaphyllum, 8-12 parts of fructus forsythiae, 4-8 parts of folium isatidis, 4-8 parts of honeysuckle, 12-20 parts of platycodon grandiflorum, 8-12 parts of perillaseed, 4-8 parts of codonopsis pilosula, 4-8 parts of scutellaria baicalensis and 4-8 parts of radix sophorae flavescentis. The Chinese herbal medicines are decocted into liquid medicine to feed poultry, bronchitis and complications caused by virus variant infection can be effectively treated, the physique of the poultry is enhanced, the immunity of the poultry is improved, the death rate of the poultry is reduced, the Chinese herbal medicines are decocted into the liquid medicine, feeding is easy, cost is low, and the Chinese herbal medicines are suitable for large-scale popularization and application. Even if the composition is taken for a long time, drug resistance generated in poultry bodies is low, and drug property residual quantity is small.

This invention discloses the application of Akkermansia in the preparation of drugs for the prevention and treatment of avian infectious bronchitis virus (AIB). Through research, this invention has found that Akkermansia can improve the survival rate of chickens infected with AIB and inhibit the replication of AIB. This demonstrates that Akkermansia has important applications in the preparation of drugs for the prevention and / or treatment of AIB infection, providing a new solution for the treatment of AIB infection, and can be specifically applied in drugs or feed additives for combating AIB infection.

The invention belongs to the technical field of poultry disease prevention and treatment, and discloses application of a rhizoma kaempferiae extract in preparation of a medicine for resisting avian infectious bronchitis virus. The rhizoma kaempferiae extract is prepared by taking rhizoma kaempferiae as a raw material and ethanol as an extraction solvent through extraction, filtration and concentration. Experimental results show that the rhizoma kaempferiae extract has no obvious toxicity to chick embryos within a certain dosage range; after the chicken embryo is infected with the infectious bronchitis virus, the survival rate of the chicken embryo can be improved by treating the chicken embryo with different doses of the rhizoma kaempferiae extract, and the rhizoma kaempferiae extract can reduce the virus antigen expression level and virus copy number in the liver tissue and allantoic fluid of the chicken embryo, so that the chicken embryo has prevention and treatment effects on infectious bronchitis virus infection. The rhizoma kaempferiae extract has the advantages of easily available raw materials, simple process and low cost, and is suitable for popularization and application.

The invention provides a QX type avian infectious bronchitis virus mRNA vaccine and application thereof. The gene sequence of the mRNA vaccine molecule is shown as SEQ ID NO.9 or SEQ ID NO.13. The invention also provides a preparation method of the QX type avian infectious bronchitis virus mRNA vaccine. The preparation method of the QX-type avian infectious bronchitis virus mRNA vaccine molecule provided by the invention is simple to operate, preparation can be performed only by knowing a gene sequence, and compared with an inactivated vaccine, the production price is low, and the dosage is small; compared with attenuated live vaccines, the vaccine is short in production period and safe.

A novel infectious bronchitis virus (IBV) serotype, to isolated attenuated IBV strains derived therefrom, and to live or inactivated vaccines against infectious bronchitis made using the isolated attenuated IBV strain.

The invention relates to a quadruple fluorescent PCR (Polymerase Chain Reaction) primer probe combination and a kit for an infectious bronchitis virus. The invention belongs to the field of biology, and discloses a quadruple fluorescent PCR (Polymerase Chain Reaction) primer probe combination for GI-13 / GI-19 / GVI-1 typing and general detection of an infectious bronchitis virus. Comprising a first primer probe combination for distinguishing an infectious bronchitis virus from other viruses, a second primer probe combination for identifying a GI-13 type infectious bronchitis virus, and a third primer probe combination for identifying a GI-19 type infectious bronchitis virus, and the fourth primer probe combination is used for identifying the GVI-1 type avian infectious bronchitis virus. The PCR primer probe combination is suitable for an RT-qPCR detection system, and can be used for effectively distinguishing GI-19, GI-13 and GVI-1 type IBV viruses; meanwhile, the invention further discloses a kit.

The invention provides a construction method and application of a chicken fallopian tube embolism model, and belongs to the technical field of animal infection models. The invention provides a construction method of a chicken fallopian tube embolism model based on multi-pathogen infection. The mycoplasma synoviae, the infectious bronchitis virus and the riemerella anatipestifer are utilized to infect the chicken, and the clinical typical salpingemphasis pathological process including cheese-like substance accumulation in a salpingemphasis cavity, serious abdominal air sacculitis complications and related tissue inflammation changes can be accurately reproduced. The model constructed by the invention is high in morbidity, and is suitable for epidemic disease pathogenesis research, drug screening and vaccine potency test. Compared with an existing single pathogen infection model, the model is closer to a clinical reality multi-pathogen cooperative infection scene, and the blank of a standardized multi-pathogen mixed infection induced fallopian tube embolism animal model is filled up.

The application provides a general avian infectious bronchitis virus whole genome deep sequencing method and application. The method realizes efficient amplification of long fragments of whole genomes of different genotype IBV epidemic strains by designing and applying a set of combinations containing 24 pairs of specific primers. The specific sequences of the primer combinations are shown in SEQ NO:1 to SEQ NO:48. Compared with a traditional sequencing method, the application significantly reduces the sequencing cost. Only two PCR reactions are needed, and more than 27 kb of IBV full-length genome sequence can be successfully obtained, which provides a more economical and efficient solution for IBV related scientific research and molecular epidemiology monitoring, and helps to promote the development of related field scientific research.

The invention provides a six-joint inactivated vaccine for poultry as well as a preparation method and application of the six-joint inactivated vaccine, and belongs to the technical field of veterinary vaccines. The six-combined inactivated vaccine for poultry is prepared from an inactivated newcastle disease virus antigen concentrated solution, an inactivated infectious bronchitis virus antigen concentrated solution, an inactivated H3 subtype avian influenza virus antigen concentrated solution, an inactivated H9 subtype avian influenza virus antigen concentrated solution, an inactivated I-group 4-type avian adenovirus solution, an inactivated H3 subtype avian influenza virus antigen concentrated solution, an inactivated H3 subtype avian influenza virus antigen concentrated solution, an inactivated H3 subtype avian influenza virus antigen concentrated solution, an inactivated H3 subtype avian influenza virus antigen concentrated solution and an inactivated H3 subtype avian influenza virus antigen concentrated solution. The inactivated avian infectious laryngotracheitis virus gD protein liquid is pretreated, then mixed according to a step-by-step mixing method and emulsified, and the chicken infectious laryngotracheitis virus gD protein liquid is prepared. According to the vaccine, through the innovative preparation process and technical means, the optimization of the antigen proportion and the mixing sequence and the adjustment of reaction environment parameters, the structural change or antigenicity reduction caused by the interaction between antigens is effectively avoided, the stable and effective structure of each antigen in the vaccine can be ensured, and finally the immune effect of the vaccine is not influenced.

DEPCT64 This invention relates to 4 / 91IBV (Infectious Bronchitis Virus) which is coded for... Heterologous S (spike) protein or fragments thereof; furthermore, this invention also... This is related to the components that elicit an immune response, which include the aforementioned 4 / 91IBV. Process the code for heterologous S (spike) protein or fragments thereof. This invention also relates to a method for immunizing targets, which includes: Providing the target with an element that induces an immune response to this invention. Furthermore, this invention relates to methods for treating or preventing clinical symptoms caused by IBV. The necessary objectives involve a method of providing the target with the required quantity. The therapeutic efficacy of the immune response-inducing components according to this invention. -----------------------------------------------------------

Disclosed in the present invention are a dimethylphosphoryl substituted compound, and a preparation method therefor, a pharmaceutical composition thereof and the use thereof. Provided in the present invention is a compound of formula (I) or a pharmaceutically acceptable salt thereof. The compound of the present invention can target a host eIF2α kinase, eIF2α phosphorylation and protein synthesis. The compound of the present invention has great biological activity for treating breast cancer. Particularly, compound SIPI-27 and compound SIPI-28 of the present invention has inhibitory activity against breast cancer significantly superior to that of the control compounds BTdCPU and neratinib; and / or the compound of the present invention also has biological activity against coronaviruses and / or orthopoxviruses, in particular, compound SIPI-25 of the present invention has broad-spectrum anti-coronavirus activity against porcine epidemic diarrhea virus (PEDV), mouse hepatitis virus (MHV), infectious bronchitis virus (IBV) and porcine deltacoronavirus (PDCoV), and also has activity against the Taterapox virus (TTV) orthopoxvirus.

The invention belongs to the technical field of genetic engineering, and particularly relates to an mRNA vaccine based on an infectious bronchitis virus S protein RBD segment. The active ingredient of the vaccine provided by the invention is mRNA (messenger ribonucleic acid) as shown in SEQ ID NO.1 or SEQ ID NO.2. Specific DNA molecules are obtained through a series of sequence design and sequence optimization, specific recombinant plasmids are further obtained, the recombinant plasmids are linearized and recycled and then transcribed into mRNA in vitro, modification such as capping and tailing is carried out, purification is carried out, then mRNA preparation is completed, the mRNA and lipid nanoparticles are mixed and then self-assembled into the lipid nanoparticles loaded with IBV S1-RBD mRNA, and the IBV S1-RBD mRNA lipid nanoparticles loaded with IBV S1-RBD mRNA are obtained. As a novel vaccine, the vaccine can provide a complete protection effect on SPF chickens infected by IBV, and has a good application prospect.

The present invention relates i.a. to an IBV (infectious bronchitis virus) deposited with the BVR of IZSLER under accession number DPS RE RSCIC 16, any descendant IBV thereof and any IBV having all of the identifying characteristics of the deposited IBV. Further, the present invention relates to an immunogenic composition comprising said deposited IBV.

The invention discloses an infectious bronchitis virus strain of which the microbial preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.46468. The vaccine strain has good immunogenicity. The invention also discloses an inactivated vaccine composition taking the virus strain as an immunogen, and a preparation method and application of the inactivated vaccine composition. The vaccine composition has good immunogenicity and immune protection capability.

The invention discloses a primer probe composition for detecting AIV, NDV virulent strains and IBV, a kit and application, and belongs to the technical field of fluorescence detection. The sequence of the primer probe combination is shown as SEQ ID NO.1-SEQ ID NO.9, the primer probe combination comprises a primer pair and a probe for detecting the avian influenza virus, the Newcastle disease virus virulent strain and the avian infectious bronchitis virus, and the primer probe combination can be used for detecting and distinguishing all subtypes of the avian influenza virus and the Newcastle disease virus virulent strain and different genotypes of the avian infectious bronchitis viruses. The kit is high in specificity and sensitivity, does not have cross reaction with other common viruses, and is high in detection sensitivity, and the minimum detectable quantity of the three viruses is 0.8 copy, so that the specific, sensitive and rapid detection of AIV, NDV and IBV can be realized, and the defects of the conventional detection technology are overcome. The method has important practical significance on timely blocking epidemic situation propagation, reducing breeding loss, guaranteeing public health safety and the like.

This invention discloses a primer composition, kit, and application for avian pathogens. The primer composition comprises a crRNA with the sequence shown in SEQ ID NO: 1, an RT-RAA upstream primer with the sequence shown in SEQ ID NO: 2, and an RT-RAA downstream primer with the nucleotide sequence shown in SEQ ID NO: 3. It may also include a fluorescent probe with the sequence TTTTT. This primer composition can be used for the detection of avian infectious bronchitis virus, exhibiting high specificity and no cross-reactivity with common avian viruses such as avian influenza virus, Newcastle disease virus, and infectious laryngotracheitis virus. Furthermore, the primer composition has high sensitivity, with a detection limit as low as 4.25 × 10⁻⁶. 2 The primer composition exhibits high copy number / μL, good reproducibility, and stable results. It is suitable for single-tube isothermal nucleic acid amplification detection, offering mild reaction conditions and significantly reduced detection time, demonstrating excellent industrial application value.

The invention relates to the field of preparation of poultry vaccines, in particular to a preparation method of a chicken newcastle disease and avian influenza combined vaccine, which comprises the following steps: respectively inoculating susceptible chick embryos with Newcastle disease virus, infectious bronchitis virus and avian influenza virus, and harvesting infected chick embryo liquid to obtain first virus liquid, second virus liquid and third virus liquid; inoculating the susceptible duck embryo with the egg drop syndrome virus, and harvesting infected duck embryo liquid to obtain a fourth antigen; and concentrating the harvested first virus liquid, second virus liquid, third virus liquid and fourth antigen, inactivating, and mixing and emulsifying with an adjuvant. The vaccine prepared by the invention does not generate any adverse reaction to chickens, is high in experimental safety, can induce high-level neutralizing antibodies in immunized chickens, and can effectively reduce the incidence of Newcastle disease virus, infectious bronchitis virus, egg drop syndrome and avian influenza.

The invention relates to the technical field of virology, in particular to an infectious bronchitis virus CK / CH / FJ-B3 / 2023 capable of causing immunosuppression, and the preservation number of the infectious bronchitis virus CK / CH / FJ-B3 / 2023 is CCTCC (China Center For Type Culture Collection) NO: V202563. The CK / CH / FJ-B3 / 2023 is separated from an immune tissue bursal disease of a chicken flock immunized with an infectious bronchitis vaccine, and is separated and purified to identify that the pathogen is an infectious bronchitis virus, and the genotype is GI-19. CK / CH / FJ-B3 / 2023 infected 7-day-old SPF chicken can cause atrophy and pathological injury of bursa of Fabricius besides typical respiratory tract symptoms, and the bursa of Fabricius can expel toxins for a long time and cause immunosuppression. The CK / CH / FJ-B3 / 2023 can be used for researching the pathogenesis of immune injury caused by the infectious bronchitis virus, can be used for evaluating the effectiveness of the existing chicken vaccine, and can also be used as a candidate vaccine strain of an inactivated vaccine. The CK / CH / FJ-B3 / 2023 has important significance in the prevention and control of epidemic diseases in clinical culture.

The invention relates to the technical field of gene engineering, in particular to an avian infectious bronchitis virus system based on double reporter genes and application thereof. According to the avian infectious bronchitis virus system based on the double reporter genes, real-time monitoring of IBV infection and replication can be achieved, the detection sensitivity and specificity are improved, meanwhile, experimental operation is simplified, and cost is reduced. The system can be used for various applications including but not limited to virus susceptible cell testing, virus entry mechanism research, antiviral drug screening, vaccine and neutralizing antibody effect evaluation, virus intrusion inhibitor evaluation and influence research of virus variation on infectivity. The system and the method provided by the invention provide a new tool for research and prevention and control of IBV and other enveloped viruses, and have important scientific and practical application values.

The present invention refers in general to the field of veterinary medicine and especially to the development of vectored vaccines using the Gallid alphaherpesvirus 3 (GaHV-3) or Marek's disease serotype 2 (MDV-2) vector, which contains protective antigens against different avian pathogens such as Newcastle disease virus (NDV), avian infectious laryngotracheitis virus (ILTV), infectious bursal disease virus (IBDV) or Gumboro disease virus, avian influenza virus (AIV), infectious bronchitis virus (IBV).

The present invention discloses a rapid detection kit for avian infectious bronchitis virus based on the CRISPR-Cas12a system, belonging to the technical field of detection reagents. The rapid detection kit for avian infectious bronchitis virus based on the CRISPR-Cas12a system disclosed by the present invention includes a fluorescent DNA reporter molecule and / or a lateral flow strip DNA reporter molecule, a crRNA, and upstream and downstream primers for isothermal amplification. The present invention has successfully established a rapid detection kit for IBV nucleic acid based on the CRISPR-Cas12a system, realizing rapid, sensitive and specific detection of IBV nucleic acid.

This invention discloses a chicken infectious bronchitis virus (IBBV) nanoparticle, its preparation method, and its application, belonging to the field of poultry vaccine technology. The nanoparticles are formed by the self-assembly of an SRBD-SpyTag fusion protein and a Ferritin-SpyCatcher fusion protein via a SpyTag / SpyCatcher covalent linkage system. The SRBD-SpyTag fusion protein is formed by fusing a SpyTag sequence to the N-terminus or C-terminus of the S1 protein receptor-binding domain of IBV. The nanovaccine prepared from these nanoparticles exhibits high safety, rapid antigen module replacement, and is suitable for preventing IBV infection. The technical solution of this invention has advantages such as structural stability, strong immunogenicity, and rapid iteration, providing a novel vaccine solution for poultry disease control.

Workflows for the efficient identification of viral epitopes and / or host paratopes are provided. The workflows leverage artificial intelligence to quickly and reliably identify candidate epitopes for immunogen development thereby reducing the lead time of vaccine development. Immunogenic compositions for use in the treatment and / or prevention of porcine reproductive and respiratory syndrome virus (PRRSV) and Infectious Bronchitis Virus (IBV) are also provided, as are antibodies or antigen binding fragments.

The invention relates to the technical field of molecular biology detection, in particular to an MALDI-TOF MS (matrix-assisted laser desorption / ionization-time of flight mass spectrometry) detection method for simultaneously detecting avian influenza virus (AIV), Newcastle disease virus (NDV), avian infectious laryngotracheitis virus (ILTV), avian infectious bronchitis virus (IBV), fowl pox virus (FPV), fowl adenovirus (FAdV) and fowl metapneumosis (AMPV), and simultaneously can perform preliminary typing on the AIV. The MALDI-TOF MS-based detection method for multiple detection of the poultry epidemic disease viruses has the characteristics of high sensitivity, high specificity, high flux, good repeatability and high accuracy, and can be used for qualitatively detecting three types of subtype viruses of the seven viruses and the avian influenza virus (AIV) in an actual sample. The invention provides a novel and reliable technical method for trace early warning and monitoring of early infection of ten poultry epidemic disease viruses, differential diagnosis of other common viruses, research of propagation rules of the viruses, epidemiological risk assessment and other systematic applications.

This invention relates to the field of biotechnology, specifically disclosing a detection element, kit, and detection method for detecting infectious bronchitis virus (IBV) in chickens. The detection element is a Toehold Switch element, which is an S4-3 element and / or an S3 element; the nucleotide sequence of the S4-3 element is shown in SEQ ID NO: 1; the nucleotide sequence of the S3 element is shown in SEQ ID NO: 2. The detection element of this invention features high sensitivity and strong specificity, enabling the differentiation of three IBV strains—P65, MH20, and H120—at a single-base resolution.