21 results about "Infectious bronchitis" patented technology

This invention discloses a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, its preparation method, and its application, belonging to the field of traditional Chinese medicine composition technology. The raw materials of this traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, by mass percentage, include 8%-12% extract of honeysuckle branches and leaves, 8%-12% extract of dipsacus root, 8%-12% chrysanthemum, 4%-6% mulberry leaves, 4%-6% licorice root, 0.8%-1.2% peppermint, and 50.8%-67.2% anhydrous glucose. It has good anti-inflammatory effects and can effectively alleviate inflammatory responses caused by LPS. In particular, it has good therapeutic and ameliorative effects on the clinically prevalent porcine reproductive and respiratory syndrome (PRRS) and infectious bronchitis in chickens, effectively reducing morbidity and mortality, and improving production performance.

The present application belongs to the field of veterinary medicine, and specifically discloses a traditional Chinese medicine composition for preventing and treating chicken infectious bronchitis, a preparation method and application thereof. The traditional Chinese medicine composition is composed of the following raw materials in parts by weight: Ephedra 12 parts, apricot kernel 12 parts, licorice 9 parts, coix seed 30 parts, forsythia 12 parts, honeysuckle 20 parts, white eupatorium 12 parts, smilax 20 parts, and purple violet 6 parts. The composition can be used for preventing and treating artificially infected IBV chicks, and can significantly improve the survival rate of chicks, alleviate the decrease in body weight growth caused by IBV, relieve clinical symptoms, reduce the viral load in trachea and kidney tissues, reduce organ pathological damage, and promote the recovery of the body. The traditional Chinese medicine composition for preventing and treating chicken infectious bronchitis is easy to obtain, rich in resources, low in cost, safe, non-toxic and non-residual, and the preparation method is simple and suitable for large-scale production.

The invention relates to an infectious bronchitis subunit vaccine based on a nano-skeleton technology. Specifically, the invention provides a fusion protein which sequentially comprises an infectious bronchitis virus S protein fragment or a mutant thereof, a connecting peptide and helicobacter pylori ferritin from an N terminal to a C terminal, wherein the infectious bronchitis virus S protein fragment is S1 protein, and the amino acid sequence of the S protein fragment is as shown in SEQ ID NO. 2; the mutant of the chicken infectious bronchitis virus S protein fragment is S1-2 protein or S1-3 protein, the amino acid sequence of the S1-2 protein is shown as SEQ ID NO.4, and the amino acid sequence of the S1-3 protein is shown as SEQ ID NO.5. The invention further discloses a preparation method of the chicken infectious bronchitis virus S protein fragment.

The application discloses a kind of avian influenza and avian infectious bronchitis two-union recombinant subunit vaccine and its preparation method and application.The recombinant protein is by replacing the head domain of AIV HA protein with the RBD domain of IBV S protein, while adding GCN4, Trimer-tag trimer tag in sequence, the recombinant protein obtained, the recombinant protein is expressed in vitro using insect cells and prepared into subunit vaccine.The vaccine prepared by the application can produce high-level neutralizing antibodies against homologous and heterologous H9N2 subtype avian influenza virus after immunizing chicken population, while significantly reducing the virus discharge level and the histopathological changes caused, and can also induce high-level specific antibodies and neutralizing antibodies against QX subtype avian infectious bronchitis virus, effectively inhibit discharge, viral load, and reduce tissue lesions.The application does not depend on chicken embryo, and has low production cost and short cycle, and can simultaneously prevent avian influenza and avian infectious bronchitis.

The invention provides a construction method of engineered lactic acid bacteria for constitutive expression of SodA protein and application of the engineered lactic acid bacteria in treatment of IBD. The invention provides a construction method of engineered lactic acid bacteria (KI (at) lSodA) for relieving IBD (Infectious Bronchitis Disease) constitutive expression SodA protein. A verification experiment result shows that the KI (at) lSodA highly expresses the SodA protein, and the oxidative stress management capability of the strain is improved; meanwhile, the KI (at) lSodA also has long-term use safety, the treatment of the KI (at) lSodA remarkably relieves the colitis symptoms and in-vivo inflammation conditions of mice in an acute stage, a recovery stage and a chronic state, and the KI (at) lSodA shows a strong intestinal microbial diversity recovery capability in all three experimental models, and particularly shows a remarkable repair effect in a recovery stage model. Therefore, the invention provides a new basis for IBD treatment.

The invention discloses an avian influenza and avian infectious bronchitis bigeminy recombinant subunit vaccine as well as a preparation method and application thereof. The recombinant protein is obtained by replacing a head structural domain of an AIV HA protein with an RBD structural domain of an IBV S protein and adding GCN4 and Trimer-tag trimer tags into a sequence, and the recombinant protein is expressed in vitro by using insect cells and is prepared into a subunit vaccine. After chicken flocks are immunized by the prepared vaccine, high-level neutralizing antibodies aiming at homologous and heterologous H9N2 subtype avian influenza viruses can be generated, meanwhile, the detoxification level of the viruses and caused tissue pathological changes are remarkably reduced, and high-level specific antibodies and neutralizing antibodies aiming at QX subtype avian infectious bronchitis viruses can also be induced; toxin expelling and virus load are effectively inhibited, and tissue lesion is relieved. The method does not depend on chick embryos, is low in production cost and short in period, and can prevent avian influenza and avian infectious bronchitis at the same time.

The invention relates to the technical field of virology, in particular to an infectious bronchitis virus CK / CH / FJ-B3 / 2023 capable of causing immunosuppression, and the preservation number of the infectious bronchitis virus CK / CH / FJ-B3 / 2023 is CCTCC (China Center For Type Culture Collection) NO: V202563. The CK / CH / FJ-B3 / 2023 is separated from an immune tissue bursal disease of a chicken flock immunized with an infectious bronchitis vaccine, and is separated and purified to identify that the pathogen is an infectious bronchitis virus, and the genotype is GI-19. CK / CH / FJ-B3 / 2023 infected 7-day-old SPF chicken can cause atrophy and pathological injury of bursa of Fabricius besides typical respiratory tract symptoms, and the bursa of Fabricius can expel toxins for a long time and cause immunosuppression. The CK / CH / FJ-B3 / 2023 can be used for researching the pathogenesis of immune injury caused by the infectious bronchitis virus, can be used for evaluating the effectiveness of the existing chicken vaccine, and can also be used as a candidate vaccine strain of an inactivated vaccine. The CK / CH / FJ-B3 / 2023 has important significance in the prevention and control of epidemic diseases in clinical culture.

A bacteria-based compound for the prevention and treatment of a number of strains of avian influenza via oral intake by way of feed or drinking water or both. The agent is an inventive treatment compound comprising a bacterial-based culture which is directed to the prevention and treatment of avian influenza primarily for use in commercial birds such as poultry. The inventive treatment compound disclosed herein is produced from a lipopolysaccharide of a Gram-negative bacterial strain of the Brevundimonas group. The compound may be delivered to poultry by way of liquid or dry feed according to an effective prescribed regimen. A similar treatment approach may be taken as well after the bird is infected. Use of the composition is effective in the prevention and treatment of a variety of avian influenza, including high pathogenicity avian influenza, low pathogenicity avian influenza, Newcastle disease virus, infectious bronchitis disease virus, and infectious laryngotracheitis virus.

The invention belongs to the technical field of poultry vaccines, and particularly relates to a chicken new tributary method adeno-quintuplet vaccine and a preparation method thereof.The chicken new tributary method adeno-quintuplet vaccine comprises chicken Newcastle disease venom, infectious bronchitis venom, avian influenza venom, infectious bursal disease virus liquid and fowl adenovirus venom, and the preparation method of the Newcastle disease venom comprises the steps that firstly, frozen MDCK suspension cells are provided; the frozen MDCK suspension cells are recovered at the temperature of 40-45 DEG C; 2, transferring the resuscitated MDCK suspension cells to a serum-free culture medium; according to the invention, the inactivated vaccine is prepared from the Newcastle disease venom, the infectious bronchitis venom, the avian influenza venom, the infectious bursal disease virus liquid and the fowl adenovirus venom by adopting a superstrong concentration process and a high-quality adjuvant, so that after animals are immunized, antibodies are generated quickly, the titer is high, the protection period is long, and the occurrence and spreading of epidemic diseases are prevented; and people can conveniently put multi-combined vaccines to cope with continuously changing epidemic disease prevention and control situations.

The invention belongs to the field of veterinary drugs, and relates to an infectious bronchitis nanoparticle antigen and a vaccine thereof. The avian infectious bronchitis nanoparticle antigen is a helicobacter pylori ferritin nanoparticle with an avian infectious bronchitis virus S1 protein fragment. The nanoparticle vaccine comprises an immunizing dose of the avian infectious bronchitis nanoparticle antigen and a pharmaceutically acceptable carrier. The vaccine has the excellent effects of being high in safety, good in immunogenicity, stable in batches and capable of providing complete protection for attacking the infectious bronchitis virus.

The application discloses a compound traditional Chinese medicine, a preparation method and application thereof in treating chicken infectious bronchitis, and aims at the pathogenic characteristics of chicken infectious bronchitis, combines traditional Chinese medicine theory and prescription rules, and prepares a compound traditional Chinese medicine which can treat chicken infectious bronchitis, and the compound traditional Chinese medicine is prepared from the following raw materials: Radix Stemonae, Platycodonis, Glycyrrhizae, Eriobotryae and Rehmanniae. The compound traditional Chinese medicine has the effects of relieving cough and reducing sputum, clearing heat and resolving toxin, and benefiting water and removing dampness through scientific compounding of monarchs, ministers, aids and messengers. The main highlight of the application is to improve the immune response level of the body, and the chicken infectious bronchitis virus is prevented from further invading the lung, and the treatment effect is good. The compound traditional Chinese medicine has obvious treatment effect on chicken infectious bronchitis, the method is simple and easy to implement, is green and safe, and has important significance for reducing the use of antiviral chemicals in breeding production.

The invention discloses a chicken infectious bronchitis prediction method based on an AI chip, and relates to the technical field of animal epidemic disease intelligent monitoring and prediction, and the method comprises the following steps: S001, building a nanosecond-level unified time reference, continuously collecting chicken house environment sound signals through a distributed acoustic collection unit, generating a phase and energy joint spectrum according to the time reference, and carrying out the detection of the phase and energy joint spectrum; and stripping fundamental tone and harmonic components of low-frequency mechanical vibration by using a spectral difference operator to form a dynamic reference signal with separated sound sources. According to the method, the acoustic processing link is driven through the nanosecond time base, dynamic extraction and reconstruction of cough sound are achieved, a false alarm path is recognized in combination with residual density and confidence gating, an interference field is constructed through inverse sound wave injection, the recognition threshold value is dynamically adjusted, and the prediction precision and stability of an AI chip in a high-noise environment are improved.

The invention relates to the field of preparation of poultry vaccines, in particular to a vaccine composition for resisting Newcastle disease, infectious bronchitis and avian influenza. 5 to 15 parts of vitamin E; 2 to 10 parts of levamisole; 1-10 parts of a divalent inorganic salt; 1-5 parts of a complexing agent; 20-40 parts of a first bacterial liquid containing inactivated avian influenza virus; 20-30 parts of a second bacterial liquid containing inactivated infectious bronchitis virus; 10-30 parts of a third bacterial liquid containing inactivated Newcastle disease virus; 1 to 3 parts of aluminum stearate; and 10 to 15 parts of an oil phase. The vaccine prepared by the invention does not generate any adverse reaction to chickens, is high in experimental safety, and can induce high-level neutralizing antibodies in immunized chickens, which indicates that the immunized chickens can effectively resist virus attacks of Newcastle disease virus, avian influenza and infectious bronchitis virus.

The present invention relates to oligosaccharide preparations for treating, alleviating, preventing, and / or delaying respiratory diseases, preferably infectious bronchitis, and / or their effects.

The invention discloses an IBV (Infectious Bronchitis Virus) N (Infectious Bronchitis Virus)-protein-targeted natural medicine for resisting avian infectious bronchitis virus as well as a screening method and application thereof. The unique active ingredient of the natural medicine is salvianolic acid B (the CAS number is 115939-25-8, and the purity is greater than or equal to 99.92%), and the natural medicine can be specifically combined with a 10 * 10 * 10 active pocket (coordinates X are equal to 12.3, Y is equal to 8.7 and Z is equal to 10.2) of IBV M41 strain N protein, so that virus replication is remarkably inhibited. The screening method adopts a three-stage process of computer virtual screening, chick embryo in-egg verification and in-vivo and in-vitro confirmation, and drug activity and safety are ensured through layer-by-layer screening of molecular docking, chick embryo experiments, cell experiments and chick in-vivo experiments. The medicine is high in safety (the survival rate of CEK cells is larger than or equal to 92.5%, and no obvious toxicity exists), remarkable in antiviral effect (the chicken virus load is reduced by 70%-82%, and the clinical symptom remission rate is larger than or equal to 50%), capable of being orally taken (10-20 mg / kg body weight, one treatment course of 7 days) and various in dosage form (powder, oral liquid and granules), is used for preparing the avian infectious bronchitis resisting medicine, fills the market blank and has important application value.

The invention discloses a compound traditional Chinese medicine aqueous extract for preventing and treating infectious bronchitis as well as a preparation method and application thereof, and belongs to the field of veterinary traditional Chinese medicine preparations. The aqueous extract is prepared from rhizoma pinellinae praeparata, bulbus fritillariae thunbergii, pericarpium citri reticulatae, liquorice, platycodon grandiflorum and semen armeniacae amarae according to a specific proportion through water extraction and concentration. Aiming at the defects of an existing prevention and treatment scheme in recovery of the growth performance and immune function of the infected chicken, the invention innovatively adopts a treatment rule formula of combining qi regulating, spleen strengthening, dampness eliminating, phlegm reducing and lung qi diffusing and lowering. Animal experiments show that the compound can effectively inhibit avian infectious bronchitis virus replication, can significantly improve the thymus index of infected chicks, and enables the average daily gain of the chicks to recover to a level that the average daily gain is not significantly different from that of a healthy group, and the synergistic effect is significantly higher than that of a ribavirin positive control group. The composition is simple in component, stable in process and good in safety, and a new product is provided for green and efficient prevention and treatment of infectious bronchitis.

The invention belongs to the technical field of chicken disease prevention, and particularly relates to a quadruple inactivated vaccine for preventing chicken diseases, which is prepared by respectively proliferating four viruses, namely a newcastle disease virus LaSota strain, an infectious bronchitis virus M41 strain, an infectious bursal disease virus BJQ902 strain and a viral arthritis virus T98 strain, on an SPF chicken embryo and a chicken embryo fibroblast to obtain a virus solution, the preparation method comprises the following steps: carrying out 2-4 times concentration, inactivating by using a formaldehyde solution, proportioning according to a certain ratio, emulsifying, preparing an inactivated vaccine, carrying out safety and immune effect tests on the inactivated vaccine by using SPF (specific pathogen free) chickens, carrying out serology and challenge protection tests on the inactivated vaccine, carrying out physical property detection on the prepared inactivated vaccine, and continuously observing for 14 days, according to the present invention, the immune effect test results show that the Newcastle disease antibody level can achieve more than 6.0 log2 after the immunization for 28 d, and the antibody titer after the secondary immunization of the infectious bronchitis is 9 times of the first immunization of the infectious bronchitis;

This invention discloses a traditional Chinese medicine oral liquid for treating infectious airsacculitis in chickens and its preparation method, belonging to the field of traditional Chinese veterinary medicine preparation technology. The oral liquid is composed of thirteen traditional Chinese medicines: buckwheat, houttuynia cordata, isatis root, honeysuckle, forsythia, scutellaria baicalensis, gypsum, ephedra, apricot kernel, platycodon grandiflorus, fritillaria cirrhosa, mulberry bark, and licorice. This formula has the effects of clearing heat and detoxifying, relieving lung congestion and asthma, and resolving phlegm and relieving cough. This invention uses modern traditional Chinese medicine extraction technology, extracting volatile oils from some medicinal materials through steam distillation and extracting others through ethanol reflux extraction. The extracts are combined, and suitable excipients are added to prepare an oral liquid preparation. This invention overcomes the drawbacks of traditional powders, such as low bioavailability and slow onset of action. Clinical trials have proven that it achieves a cure rate of over 92% and an effective rate of over 96% for infectious airsacculitis in chickens, with rapid onset of action. Respiratory symptoms are significantly improved within 24 hours after administration, with no drug residue and no drug resistance.

The invention discloses a SOCS1 antagonistic peptide for preventing and treating avian infectious bronchitis and application of the SOCS1 antagonistic peptide, the amino acid sequence of the SOCS1 antagonistic peptide is LPQDKEYYKVKEP, and Y is phosphorylated tyrosine. Reasonable design is carried out on the basis of a molecular mechanism of interaction of a JAK2 activation ring and the SOCS1 protein, a molecular docking result shows that the SOCS1 antagonistic peptide can be precisely combined to KIR and SH2 functional domains of the chicken SOCS1 protein through a specific phosphorylated tyrosine residue of the SOCS1 antagonistic peptide, and the combination can competitively block combination of SOCS1 and endogenous JAK2 of cells, so that the chicken SOCS1 antagonistic peptide can be used for preparing the chicken SOCS1 antagonistic peptide. Therefore, the inhibition effect of SOCS1 on a JAK-STAT signal channel is relieved, the I-type interferon antiviral immune response of a host is recovered, and virus replication is inhibited fundamentally.

This invention relates to the field of biomedical technology, specifically to the application of forsythoside in the preparation of drugs for the prevention and treatment of infectious bronchitis in chickens. Immunofluorescence and Western blotting results show that forsythoside can significantly inhibit the expression of viral nucleocapsid proteins in CEK cells and reduce the viral load in CEK cells. Furthermore, forsythoside can protect against IBV infection in broiler chickens. Quantitative fluorescence results indicate a significant reduction in viral replication in the tracheal tissue of broilers. Clinical symptoms and histopathological results show that forsythoside can significantly alleviate IBV infection-induced slow weight gain, respiratory distress, and tracheal tissue damage in broilers. This invention demonstrates that forsythoside has low cytotoxicity, can effectively prevent infectious bronchitis in chickens, and has advantages such as being green and safe, leaving no residue, having few toxic side effects, and being unlikely to induce drug resistance.

The invention relates to the field of animal health. The present invention provides an attenuated infectious bronchitis virus (IBV). The attenuated infectious bronchitis virus can protect chickens from IBV infection.