403 results about "Infectious bronchitis" patented technology

The invention discloses a infectivity bronchitis attenuated vaccine strain LDT3-A strain, and discloses application and application effect thereof in preventing and curing chicken infectivity bronchitis. The microorganism accession number of the attenuated vaccine strain is CGMCC-2902. The attenuated vaccine strain of the present invention has good safety and good immunization protection effect to the chicken infectivity bronchitis. The attenuated strain can be prepared into single vaccine or combined vaccine for preventing or curing infectivity bronchitis virus.

The invention discloses a GeXP rapid detection kit capable of simultaneously identifying nine pathogens of chicken respiratory tract diseases. The kit is used based on a GeXP system and comprises ten polymerase chain reaction (PCR) primer pairs; the kit is used for identifying and detecting avian influenza virus, H5, H7 and H9 subtype avian influenza virus, newcastle disease virus, infectious bronchitis, infectious laryngotracheitis, mycoplasma gallisepticum, bursa synovialis mycoplasma and haemophilus paragallinarum; and the kit is good in specificity, high in sensitivity and can detect 100 copy/mu l. Compared with an identifying result of the conventional experiment method of a pathogen separation and hemagglutination inhibition experiment or a serology experiment and the like, the GeXP rapid detection kit has the advantage that the coincidence rate reaches 100 percent. The kit is generally used for detecting the main chicken respiratory tract diseases and the pathogens thereof, so that a simple and high-flux detection kit and a detection system are provided, an actual requirement is met, and the application prospect is wide.

The invention relates to feed for pheasant juveniles and a preparation method thereof. The preparation method is to grind and mix 30 to 40 parts of corn, 2 to 8 parts of broomcorn, 2 to 10 parts of wheat bran, 15 to 30 parts of bean pulp, 3 to 10 parts of bean cake, 2 to 10 parts of yeasts, 5 to 15 parts of eggs, 6 to 15 parts of fish meal, 1 to 5 parts of bone meal, 0.1 to 1 part of methionine, 0.3 to 1 part of bone meal and 3 to 10 parts of Chinese medicine powder, wherein each 500 grams of Chinese medicine powder comprises 65 grams of astragalus, 100 grams of dandelion, 80 grams of honeysuckle, 80 grams of isatis root, 60 grams of folium isatidis, 30 grams of liquoric root, 30 grams of big head atractylodes rhizome, 30 grams of white paeony root, 20 grams of root of Chinese pulsatilla, 3 grams of borneol and 2 grams of realgar. In the feed for the pheasant juveniles, on the basis of ensuring the sound growth of livestock animals, according to life vulnerability of the pheasant juveniles, the mixing ratio of feed raw material components is reasonable and various Chinese medicine components are added, so that the common communicable diseases such as bursa of Fabricius, white diarrhea and infectious bronchitis of pheasant juveniles are inhibited effectively, and the death rate can reduce to 43 to 47 percent.

Disclosed is a veterinary medicine for treating virus diseases of domestic birds comprising the following constituents: honeysuckle flower 25-40 parts, capsule of weeping forsythia 25-40 parts, ledebouriella root 25-35 parts, herba schizonepetae 25-40 parts, epimedium 35-45 parts, baikal skullcap root 25-40 parts, radix isatidis 25-40 parts, dandehon herb 35-45 parts, dyers woad leaf 25-40 parts, bupleurum root 20-35 parts, cinnamon twig 25-40 parts, licorice root 25-40 parts, psoralea fruit 20-35 parts, astragalus root 35-45 parts, and cordate houttuynia 25-40 parts.

The method relates to an attenuated vaccine strain for avian infectious bronchitis virus and application thereof. According to the invention, avian infectious bronchitis virus (IBV) is attenuated, so as to successfully obtain an IBV attenuated strain CCTCC.V201232 and a derivative virus strain thereof. The attenuated strain and derivative virus strain provided by the invention can be used in preparation of a vaccine composition for prevention of infectious bronchitis. Experiments show that the attenuated strain and vaccine composition provided by the invention can be inoculated to immature birds, so as to effectively activate immune system in the birds and well prevent avian infectious bronchitis.

The invention provides a traditional Chinese medicine prepration for treating avian nephropathogenic infectious bronchitis, and the active ingredients thereof are prepared by the following raw materials according to the mixture ratio by weight: 80-120 parts of heartleaf houttuynia herb, 60-100 parts of tatarian aster root, 50-70 parts of perilla leaf, 40-60 parts of sea-ear shell, 40-60 parts of cassia seed, 35-60 parts of swordlike atractylodes rhizome, 30-60 parts of platycodon root, 30-50 parts of airpotato yam rhizome, 30-50 parts of oriental stephania root, 30-50 parts of rhubarb, 30-50 parts of membranous milkvetch root, 30-50 parts of tangerine peel, 30-40 parts of lightyellow sophora root, 20-40 parts of liquoric root, 20-40 parts of cape jasmine fruit, 20-40 parts of turmeric root-tuber, 20-40 parts of Sanxian and 20-40 parts of Chinese gentian. The medicine can effectively treat the avian nephropathogenic infectious bronchitis, the cure rate is more than 90 percent, and the medicine has difficult recurrence, obvious effect, convenient administration, no toxicity or side effects, safe use and low price. Bacteria have difficulty generating the drug resistance by using the medicine, thereby avoiding the occurrence of veterinary drug residues and social public harms.

The invention provides a preparation method for a triple inactivated vaccine of recombinant Newcastle disease, bird flu and infectious bronchitis. The technical scheme is realized by gene modification of a Newcastle disease virus (DNV) LaSota strain. A recombinant NDV low virulent strain (rLaSota strain) is taken as a vector, firstly an S1 gene of a chicken infectious bronchitis virus (IBV) is inserted between F-HN, a recombinant NDV rLaSota/S1 expressing an S1 protein is constructed by utilizing a reverse genetic technology, and a result shows that rLaSota can serve as a live vector for stably expressing the S1 protein; secondly a recombinant NDV rLaSota/S1-HA expressing an HA gene is constructed, namely, the HA gene is inserted between P-M of the recombinant NDV rLaSota/S1; and thirdly immunogenicity assessment is performed. A result shows that the triple inactivated vaccine of the recombinant Newcastle disease, the bird flu and the infectious bronchitis, prepared through a conventional inactivated vaccine process has relatively good protection effects for the Newcastle disease, the bird flu and the infectious bronchitis.

The invention relates to a traditional Chinese medicine granule which has the effects of stopping cough and calming asthma, including four medicines of ephedra herb, bitter apricot seed, gypsum and liquoric root. The invention is mainly applicable to poultry infectious bronchitis, infectious laryngotracheitis, chronic respiratory disease, infectious rhinitis and other diseases. The formula of the invention has the effects of clearing heat, removing toxicity, stopping cough and calming asthma. The medicine can be prepared into the soluable granule by the combination of the special physiological structure of short reproductive tracts of birds which can not digest the wood fiber, the invention can be dissolved rapidly in fodder drinking water, and the invention can achieve the purpose of rapid onset of action after the drinking water is absorbed by sick chickens.

The invention relates to a method for producing a combined vaccine, in particular to a method for producing a combined vaccine against Newcastle disease and infective bronchitis. The method comprises that: before culture in the same embryo, purity of breeding virus of the Newcastle disease and the infective bronchitis is improved; and during culturing in the same embryo, concentrations of two antigens in the combined vaccine against the Newcastle disease and the infective bronchitis are adjusted to reach reasonable combination. The vaccine prepared by the method is vaccinated with chicken flocks to generate ND and IB immunity without any interference. Furthermore, the vaccine strains are reasonably matched to effectively solve the problem of serious interference in simultaneous vaccination of the Newcastle disease living vaccine and infective bronchitis living vaccine, improve immunization quality and reduce the number of immunization.

Disclosed is a fowl use antiviral composition which comprises the following constituents: radix isatidis 30-50 parts, honeysuckle flower 5-15 parts, capsule of weeping forsythia 15-25 parts, coptis root 15-25 parts, atractylodes rhizome 15-25 parts, astragalus root 20-40 parts, red peony root 10-20 parts, scrophularia root 20-40 parts, Chinese angelica root 5-15 parts, medicated leaven 15 parts, malt 15 parts and licorice 15-25 parts.

The invention is concerned with a kind of bacterin to prevent fowl infectious disease, relating to a kind of bacterin preparation method to prevent Newcastle Disease and infectious bronchitis. The selected genus is Newcastle Disease virus La Sota individual plant, infectious bronchitis virus M41 individual plant and HN99 kidney type of aberrance individual plant. Dilute two kind of virus and inoculate in chick embryo allantoic cavity, collect liquid of embryo to alive and dead embryos as seed. Dilute and inoculate in allantoic cavity of affectable chick embryo to get liquid of virus embryo. Concentrate with equipment and add with formaldehyde solution to prepare bacterin through emulsion process. This invention can prevent Newcastle Disease, chick kidney type and breathing type infectious bronchitis, and it can reduce the times of injection and the cost of epidemic prevention for easy using and practicality.

The invention relates to a Chinese medicinal preparation, in particular to a formula of traditional Chinese medicine for treating respiratory diseases of chicken, which is characterized by comprising the following components in part by weight: 6 to 10 parts of indigowoad root, 3 to 12 parts of houttuynia cordata thumb, 7 to 10 parts of common andrographis herb, 3 to 6 parts of pepperweed seed, 7 to 9 parts of white mulberry root-bark, 5 to 11 parts of loquat leaf, 3 to 12 parts of ephedra, 5 to 11 parts of apricot kernel, 4 to 12 parts of platycodon root, 6 to 8 parts of baical skullcap root, 6 to 8 parts of astragalus root and 4 to 6 parts of liquorice root, and the components are mixed. The formula of the traditional Chinese medicine has the main effects of clearing heat and detoxicating, ventilating the lung and resolving phlegm and relieving asthma and relieving cough, and is mainly used for treating various respiratory diseases such as chronic respiratory diseases of the chicken, infectious bronchitis, infectious laryngotracheitis and the like.

The invention discloses a chicken new castle disease-infectious bronchitis trivalent combined vaccine and a preparation method thereof. The trivalent combined vaccine comprises antigens and a freeze-drying protective agent. The antigens comprise a chicken kidney-type infectious bronchitis virus K136 strain having an accession number of CCTCC-V201320, a chicken breath-type infectious bronchitis virus H120 strain, and a chicken new castle disease virus La Sota strain. A mode of inoculating the K136 strain and the La Sota strain in a homeomorphism manner and inoculating the H120 strain individually is adopted for preparing the antigens. Based on the mode, after culture products are mixed according to a ratio to prepare the antigens, a product of the vaccine is obtained by adding the freeze-drying protective agent. The trivalent combined vaccine can prevent the chicken new castle disease and the chicken infectious bronchitis simultaneously, thus solving effectively a problem that vaccines at present can only provide a part of protection or cannot provide protection for the kidney-type IB, and achieving an objective that one injection can be used for preventing two diseases.

The invention relates to a compound immunoenhancement agent and an application thereof on preparing a vaccine for birds. The compound immunoenhancement agent contains 5ng-10mg/mL of poly IC, 10ng-10mg/mL of muramyl dipeptide, 10ng-5mg/mL of levamisole, 10ng-5mg/mL of resiquimod and 10ng-5mg/mL of imiquimod. After the immunoenhancement agent and H5 hypotype bird flu inactivated vaccine are mixed together, antibody can be produced one week earlier, and the antibody titer is improved above 1.8log2. After chicken are immunized through H5 hypotype bird flu inactivated vaccine, H9 hypotype bird flu vaccine or infectious bronchitis vaccine which contains the compound vaccine immunoenhancement agent, the window phases of the antibody production are shortened, the antibody titer of the vaccine is improved, the immunization duration is prolonged, and the probability of infection disease is reduced.

The invention discloses an animal epidemic disease three-color fluorescence RT-PCR detection kit and a detection method thereof, and is characterized in that the kit comprises multiple fluorescence RT-PCR reaction mother liquor, a positive control, a negative control, an AMV reverse transcriptase, and a Taq polymerase, wherein the multiple fluorescence RT-PCR reaction mother liquor contains a multiple 10*fluorescence RT-PCR reaction buffer, an avian influenza primer and a probe, a newcastle disease virus primer and a probe, an avian infectious bronchitis primer and a probe, and bovine serum albumin, wherein sequences of the forward primers, reverse primers, and specific probes are as shown in SEQIDNO. 1, NO. 2, NO.3, NO.4, NO.5, NO.6, NO.7. NO.8, NO.9, and NO.10. The advantages of the invention are that avian influenza, newcastle disease, and avian infectious bronchitis viruses can be detected simultaneously in a same reaction tube; the specificity is strong; the sensitivity is high, is rapid and accurate.

The invention provides a forage for preventing the infectious bronchitis of chicken. The forage is characterized in that the forage comprises corn, soybean meal, salt, fish meal, bone meal, a trace element additive, an amino acid additive, a vitamin additive, and a traditional Chinese medicinal immune powder feed additive used for preventing the infectious bronchitis of chicken. The forage has a substantial effect on the prevention of the infectious bronchitis of chicken, and has the advantages of non-toxicity, drug resistance and no drug residual.

The invention discloses a triple egg yolk antibody against newcastle disease virus, avian influenza virus and infectious bronchitis virus and a preparation method and application thereof. The preparation method of the egg yolk antibody comprises the following steps of: (1) selecting a hen; (2) performing triple inactivation immunization of the hen obtained by the step (1) by use of newcastle disease virus, infectious bronchitis virus and avian influenza virus; (3) taking the egg laid by the hen and separating the egg yolk liquid; and (4) separating and purifying an egg yolk antibody from the egg yolk liquid obtained by the step (3). The triple egg yolk antibody disclosed by the invention is of great value in treating and preventing newcastle disease, avian influenza and infectious bronchitis.

The invention belongs to the immunity application field, which relates to the related fields of animal molecular biochemistry and the immunology, etc. The invention discloses a reagent kit respectively used for antibody of infectious bronchitis of chicken, which comprises a case body, and the test paper card and sample diluent equipped inside the case body, wherein, the test paper card is formed by the absorption pad, nitrocellulose membrane, gold-label pad and sample pad affixed in turn on the non-absorbent supporting flake by taking the sample pad, absorption pad and the NP recombinant proteins coated with the nucleocapsid of the infectious bronchitis of chicken as the detection line and the NP recombinant proteins coated with anti-mouse IgG as nitrocellulose membrane of the quality control line. The reagent kit used for testing the corresponding antibody has the obvious advantages of strong specificity, high sensitivity, easy operation and fast diagnosis.

The invention discloses an efficacy test method of infectious bronchitis inactivated vaccines, which can be applied to vaccine quality control specifically comprising challenge protection rate control and egg-laying efficacy control. Experiments prove that the invention uses the method for detecting the HI antibody titer to replace the animal challenge test, and the method has the characteristics of simpleness, convenience, rapidness, accurate results, strong repeatability, strong specificity and the like and has the significance of popularization.

The present invention relates to a preparation method of a triple inactivated vaccine for the prevention of H9 subtype newcastle disease, infectious bronchitis and avian influenza, which is characterized in that: the content of the using NDVLaSota stain virus liquid is 10 to 22 percent, the content of IBVM41 stain virus liquid is also 10 to 22 percent and the content of the AIVHL stain virus liquid is 8 to 15 percent; the present invention uses the concentration and inactivation methods; the white oil adjuvant and the immune auxiliaries of Tween, Span and aluminum stearate are added to prepare the triple inactivated vaccine; the present invention realizes the multiple vaccine prevention and achieves the purpose of prevention a plurality of diseases by one needle, which can be used for greatly developing the husbandry, so as to benefit the mankind and improve the living standard of the people.

The invention relates to a compound preparation for treating the virosis of birds and the preparation method of the compound preparation. The compound preparation is prepared by mixing raw materials by weight portions, 20 to 50 parts of milk veteh and 30 to 50 parts of grifola frondosa. Preparation procedures are as follow: The raw materials are extracted by soaking, decocted by heating and precipitated by filtering; therefore the Chinese herbal medicine is prepared. The compound preparation has the beneficial effect as follows: (1) the new preparation that can control the virosis of the birds and strengthen the disease resistance of organism is created for clinical treatment; (2) the compound mode is reasonable, the antiviral capability is strong and all the selected medicine is the effective medicine for treating the virosis of birds; (3) the compound preparation has the advantages of advanced technology, stable preparation and long storage life; (4) the compound preparation has the advantages of being safe to use, definite healing effect and controllable quality; (5) the compound preparation has the advantages of wide source of the raw materials, being avirulent, no side effect and being uneasy to cause drugfastness, so as to meet the standard of international hygienic examination. The compound preparation is especially applicable for treating infectious bursal disease, atypical newcastle disease and infective bronchitis, and the treating effect is remarkable.

The invention relates to a method for producing a quadruple inactivated vaccine for newcastle disease, infectious bronchitis, avian influenza (H9 subtype) and infectious bursal disease. In the method, the inactivated vaccine is prepared by adopting a newcastle disease virus La Sota strain, an infectious bronchitis virus M41 strain, an avian influenza virus (H9 subtype) YBF003 strain, and an Escherichia coli genetic engineering bacteria E. coil BL21/pET28a-VP2 strain which expresses an infectious bursal disease virus VP2 protein serving as production bacteria toxic species, a super concentration process and a high-quality adjuvant. After immunizing animals, antibodies are produced rapidly; the potency is high; the protection period is long; and the outbreak and the spreading of epidemic diseases are reduced.

The invention relates to a method to prepare a combined inactive vaccine of newcastle disease and infectious bronchitis vaccine for chicks. The invention is characterized in that: firstly, concentrate and inactivate the NDVLaSota virus strains (10 per cent to 22 per cent weight ratio) and IBVM41 virus strains (10 per cent to 22 per cent weight ratio) respectively; and then add white oil and immune additives including tween, span and stearic acid to prepare the combined vaccine.

The invention provides a traditional Chinese medicine microbial ecological agent for preventing and treating infectious bursal disease of chicken and a preparation method thereof. The preparation method comprises the following steps: adding water to radix isatidis, honeysuckle, forsythia, folium isatidis, humulus scandens, radix scutellariae, dangshen, ageratum, plantain, astragalus membranaceus, angelica sinensis and liquorice, boiling by using high heat, preserving heat for 30-60 minutes by using slow fire, cooling, filtering, collecting filtrate, adding white sugar, boiling, cooling to a normal temperature, then inoculating lactobacillus, zygosaccharomyces bailii and acetobacter xylinum, and fermenting for 3-7 days so as to obtain the microbial ecological agent. After the microbial ecological agent is diluted proportionally, the diluted microbial ecological agent is directly drunk by chicken. The traditional Chinese medicine microbial ecological agent provided by the invention has the effects of resisting viruses, inducing interferon, increasing the quantity of effective microbial communities in intestinal tracts, boosting immunity and improving disease resistance of chickens without toxic or side effects and the like; the preparation method is simple, convenient to use and high in viable bacteria content and has no residues. The traditional Chinese medicine microbial ecological agent provided by the invention has a certain curative effect on infectious bronchitis and henpox and relatively good market prospect.