128 results about "Infectious laryngotracheitis" patented technology

The present invention provides a novel avian herpesvirus (NAHV) vector and recombinant vaccines made therefrom that are useful to immunize avian species against Marek's disease, infectious laryngotracheitis and Newcastle disease. Methods of immunizing an avian species against Marek's disease, infectious laryngotracheitis and Newcastle disease are also provided.

The invention discloses a GeXP rapid detection kit capable of simultaneously identifying nine pathogens of chicken respiratory tract diseases. The kit is used based on a GeXP system and comprises ten polymerase chain reaction (PCR) primer pairs; the kit is used for identifying and detecting avian influenza virus, H5, H7 and H9 subtype avian influenza virus, newcastle disease virus, infectious bronchitis, infectious laryngotracheitis, mycoplasma gallisepticum, bursa synovialis mycoplasma and haemophilus paragallinarum; and the kit is good in specificity, high in sensitivity and can detect 100 copy / mu l. Compared with an identifying result of the conventional experiment method of a pathogen separation and hemagglutination inhibition experiment or a serology experiment and the like, the GeXP rapid detection kit has the advantage that the coincidence rate reaches 100 percent. The kit is generally used for detecting the main chicken respiratory tract diseases and the pathogens thereof, so that a simple and high-flux detection kit and a detection system are provided, an actual requirement is met, and the application prospect is wide.

The invention relates to a traditional Chinese medicine granule which has the effects of stopping cough and calming asthma, including four medicines of ephedra herb, bitter apricot seed, gypsum and liquoric root. The invention is mainly applicable to poultry infectious bronchitis, infectious laryngotracheitis, chronic respiratory disease, infectious rhinitis and other diseases. The formula of the invention has the effects of clearing heat, removing toxicity, stopping cough and calming asthma. The medicine can be prepared into the soluable granule by the combination of the special physiological structure of short reproductive tracts of birds which can not digest the wood fiber, the invention can be dissolved rapidly in fodder drinking water, and the invention can achieve the purpose of rapid onset of action after the drinking water is absorbed by sick chickens.

The invention discloses oral Chinese medicine liquid for treating chicken infectious laryngotracheitis. The oral Chinese medicine liquid comprises the following components by weight: 95-105g of wild chrysanthemum flower, 95-105g of hempleaf groundsel herb, 95-105g of Astragalus membranaceus, 95-105g of subprostrate sophora, 95-105g of platycodon root, 45-55g of isatis root, 45-55g of rehmannia, 15-25g of semen armeniacae amarae, 25-35g of coptis, 45-55g of Scutellaria baicalensis, 45-55g of Japanese raisintree fruit, 45-55g of honeysuckle flower, 45-55g of carthamus seed, 45-55g of garden burnet root, 15-25g of fructus forsythiae, 25-35g of fiveleaf gynostemma herb, 25-35g of gypsum and 15-25g of licorice root. The preparation technology comprises: 1. sorting; 2. superfine crushing; 3. soaking; 4. medicine liquid extraction; 5. microwave extraction; 6. filtration; 7. preservative addition; and 8. split charging. The invention has the advantages that the effect of treating chicken ILT and IB is good, the residue is low and the biological safety is good.

The invention relates to a Chinese medicinal preparation, in particular to a formula of traditional Chinese medicine for treating respiratory diseases of chicken, which is characterized by comprising the following components in part by weight: 6 to 10 parts of indigowoad root, 3 to 12 parts of houttuynia cordata thumb, 7 to 10 parts of common andrographis herb, 3 to 6 parts of pepperweed seed, 7 to 9 parts of white mulberry root-bark, 5 to 11 parts of loquat leaf, 3 to 12 parts of ephedra, 5 to 11 parts of apricot kernel, 4 to 12 parts of platycodon root, 6 to 8 parts of baical skullcap root, 6 to 8 parts of astragalus root and 4 to 6 parts of liquorice root, and the components are mixed. The formula of the traditional Chinese medicine has the main effects of clearing heat and detoxicating, ventilating the lung and resolving phlegm and relieving asthma and relieving cough, and is mainly used for treating various respiratory diseases such as chronic respiratory diseases of the chicken, infectious bronchitis, infectious laryngotracheitis and the like.

The invention discloses a traditional Chinese medicine for preventing and controlling respiratory diseases of animals, which is prepared from the following components in percentage by weight: 9 to 17 percent of liquoric root, 6 to 14 percent of gypsum, 4 to 12 percent of root of large-flowered skull, 3 to 11 percent of honeysuckle, 3 to 11 percent of sweeping forsythia, 2 to 10 percent of root bark of white mulberry, 2 to 10 percent of dried tangerine, 2 to 10 percent of tangerine seed, 2 to 10 percent of hawthorn, 2 to 10 percent of loguat leaf, 2 to 10 percent of root of straight ladybell, 3 to 7 percent of whiteflower hogfennel root, 3 to 7 percent of semen armeniacae amarae, 3 to 7 percent of rhizoma anemarrhenae, 1 to 3 percent of bryozoatum and 1 to 3 percent of borneol. By adopting the formula, from the screening of raw materials to the extraction and proportioning of active ingredients, a slow release / controlled release and targeting drug delivering technology is applied, so the medicine can effectively and quickly reach lesion sites, has the advantages of quick response, short treatment time, small side effect, convenient use, strong pertinence and high protectiveness, maintains the blood concentration in an effective treatment range, and effectively prevents and controls non typical Newcastle disease, bird flu, infectious laryngotracheitis, infectious bronchitis, colibacillus, mycoplasmosis and unexplained animal diseases such as head and face swelling and expiratory dyspnea.

The invention relates to a medicinal composition for treating avian infectious bronchitis. The medicinal composition is characterized by comprising the following medicaments in part by weight: 1 to 2 parts of honeysuckle flower, 1 to 2 parts of indigowoad root, 1 to 2 parts of weeping forsythia, 0.5 to 1 part of dandelion, 0.5 to 1 part of Chinese violet, 0.5 to 1 part of platycodon root, 0.5 to 1 part of blackberry lily rhizome and 0.4 to 0.6 part of raw liquoric root. The preparation method comprises the following steps of: weighing the eight medicaments serving as raw materials according to the weight ratio; adding water in an amount which is 4 times the amount of the medicaments; decocting twice, 1.5 hours every time; combining the decoction; filtering; standing the filtrate for 12 hours; concentrating supernatant; adding ethanol until alcohol content is 60 percent; and placing, filtering, adding water, filtering, filling and sealing and circulating steam at the temperature of 100 DEG C for 30 minutes. The medicinal composition has the advantage that the medicinal composition is a pure Chinese medicinal preparation, so that the medicinal composition does not have any residual and toxic or side effects on a body. The medicinal composition can effectively prevent infectious laryngotracheitis of chicken.

The invention discloses a lactobacillus salivarius strain and application thereof. The lactobacillus salivarius strain (with number of HVRIC5) is obtained by carrying out bacteria reproduction and enrichment on wild chicken manure in an MRS liquid selective medium and carrying out isolated culture and inoculation and purification. The microbial collection number of the strain is CGMCC No.6787. Lactobacillus salivarius has stronger acid and bile salt resistance. Antibacterial experiments in vitro indicate that the strain has stronger antibacterial effects on Gram-negative escherichia coli, salmonella and Gram-positive staphylococcus, shows a broad-spectrum antibacterial effect, has the effect on promoting growth of SPF (specific pathogen free) chickens, can effectively resist infection with salmonella and avian infectious laryngotracheitis viruses when being fed to the SPF chickens and has the effect of effectively protecting the SPF chickens.

The invention discloses a traditional Chinese medicinal composition used for preventing and treating infectious laryngotracheitis of chicken, and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines for animals. The traditional Chinese medicinal composition comprises, by weight, 20-25 parts of Radix Astragali, 20-25 parts of licorice root, 15-20 parts of Weeping Forsythia, 15-20 parts of isatis root, 15-22 parts of chrysanthemum and 30-35 parts of Folium Polygoni Tinctrorii. The composition adopting pure natural Chinese herbal medicines has the characteristics of wide medicine sources, no medicine resistance, small toxic side effects, difficult generation of harmful residues in meat products; the above medicines are compounded according to a monarch, minister, assistant and guide principle, so the composition has the efficacies of heat clearing, detoxifying, bacterium preventing, inflammation eliminating and body immunity improving; and effective components of the medicines directly participate in bacterium prevention, and can excite or enhance the specific and non-specific immune functions of bodies, so the composition is mainly used for treating the infectious laryngotracheitis of chicken.

The invention discloses a RPA primer for detecting chicken infectious laryngotracheitis virus. The nucleotide sequence of the RPA primer is shown as SEQ ID NO.1 and SEQ ID NO.2; the RPA primer is strong in specificity, high in sensitivity, and accurate in detection result. The invention further provides a RPA method for detecting the chicken infectious laryngotracheitis virus. The detecting method is easy to operate, low in equipment requirement, strong in specificity, high in sensitivity, and accurate and reliable in detection result; the detecting method provides a low-cost, fast and specific field diagnosis method for effectively detecting and diagnosing the chicken infectious laryngotracheitis virus.

The invention provides a novel vaccine with the same immunoefficiency as the prior infectious laryngotracheitis virus (ILTV) attenuated vaccine and capable of overcoming dormant infection induced by the attenuated vaccine. The invention provides the nucleotide sequence and amino acid sequence of an ILTV glycoprotein D (gD) and a preparation method, as well as a recombinant vaccine containing a viral vector (fowlpox virus vector) and ILTV gD. The fowlpox virus is obtained by homologous arm recombination of ILTV gD. The invention also provides the preparation method of the recombinant vaccine. The invention clones gD gene of ILTV Henan isolate, and performs animal experiments of the immunoprotection of the recombinant fowlpox virus. The novel vaccine has the same immunoefficiency as the prior ILTV attenuated vaccine and is capable of overcoming the shortcomings of the attenuated vaccine. The invention is convenient for large-scale production, and provides a foundation for prevention and elimination of infectious laryngotracheitis (ILT).

The invention relates to traditional Chinese medicine granules for treating infectious laryngotracheitis of chicken and a preparation method thereof. The traditional Chinese medicine granules comprise the following components according to parts by weight: 20 to 30 parts of Astragalus root, 18.5 to 28.5 parts of dyers woad leaf, 1 to 2 parts of synthetic bezoar, 1.5 to 2.5 parts of senso, 0.4 to 0.8 part of indigo naturalis, 10 to 20 parts of pig bile, 0.5 to 1.5 parts of borneol, 0.5 to 1.5 parts of natrii sulfas exsiccatus and 1 to 2 parts of liquorice. The preparation method comprises the main steps of: carrying out the processes of water extraction, dissolution, premixing, merging, last granulation and granule adjustment respectively to each component so as to prepare the finished product. According to the traditional Chinese medicine theory and scientific compatibility, the invention reserves the combined effect of the syndrome differentiation treatment of a traditional Chinese medicine prescription, utilizes a modern scientific and technological means to carry out quantitative detection and semi-quantitative detection on the effective components of a majority of medicine materials and has obvious curative effect by clinical verification.

The invention provides a feed composition for preventing and treating avian infectious laryngotracheitis. The feed composition comprises a feed additive and a feed carrier, wherein the feed additive comprises a traditional Chinese medicine composition additive which further comprises asarum, cassia twig, radices trichosanthis, radix scutellariae, honeysuckle, rhizome belamcandae, puffballs, borneol, cynanchum glaucescens, fructus gleditsiae, bamboo shavings, astragalus mongholicus, licorice roots, tuckahoe and coix seeds, and relative to 100 parts of feed carrier, the additive amount of the feed additive can be 5 parts to 9 parts. The feed composition has the advantages of high efficiency, small toxic and side effects, no residues, no drug resistance, no environmental pollution, increment of body resistance, short treatment course, quick action, low price, easiness of popularization and application, and the like on the aspects of preventing and treating chickens which suffer from avian infectious laryngotracheitis.

In the invention, a section of conserved sequences of four virus genes, namely Newcastle disease virus (NDV), infectious bronchitis virus (IBV), polymerase chain reaction (PCR) technology amplified egg drop syndrome (EDS) and infectious laryngotracheitis (ILT) by a molecular biotechnology (RT) PCR method is taken as a probe, a cellulose membrane is taken as a substrate (namely a most important basic platform in the detecting kit) to extract target pathogenic nucleic acid from pathological materials, and marked bio-16-dUTP is added during (RT) PCR to obtain a marked PCR product. The marked product is placed in the detecting kit for hybridizing detection, and quick and effective judgment and analysis on a biological sample are realized by observing changes of hybridization colors.

The invention discloses a lactobacillus salivarius strain and application thereof. The lactobacillus salivarius strain (with number of HVRIC4) is obtained by carrying out bacteria reproduction and enrichment on wild chicken manure in an MRS liquid selective medium and carrying out isolated culture and inoculation and purification. The microbial collection number of the strain is CGMCC No.6786. Lactobacillus salivarius has stronger acid and bile salt resistance. Antibacterial experiments in vitro indicate that the strain has stronger antibacterial effects on Gram-negative escherichia coli, salmonella and Gram-positive staphylococcus, shows a broad-spectrum antibacterial effect, has the effect of promoting growth of SPF (specific pathogen free) chickens, can effectively resist infection with salmonella and avian infectious laryngotracheitis viruses when being fed to the SPF chickens and has the effect on effectively protecting the SPF chickens.

The invention relates to a traditional Chinese medicine oral liquid for curing respiratory tract infection of poultries, which comprises five types of grasses including Chinese ephedra, baikal skullcap root, isatis root, cortex mori radicis and liquorice. The medicine oral liquid is suitable for curing respiratory tract infection of poultries, such as infective bronchitis, infectious laryngotracheitis, etc. Based on the fact thatthe specific physiological structure of poultries can not digest wood fibre owing to the short genital tracts, the five medicaments are made into a dosage form of oral liquid which can be dispersed in drinking water and can be taken by sick poultries through drinking water, thereby being convenient for absorbing, and achieving the purpose of taking effect rapidly.

The invention discloses a multi-fluorescent immunoassay method for rapidly distinguishing 6 types of poultry respiratory pathogens. The multi-fluorescent immunoassay method is simple to operate; a target amplified fragment is obtained through a PCR (Polymerase Chain Reaction); then an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized; an MFI (Mean Fluorescence Intensity) value is read through a detector to distinguish viruses of different types. According to the method disclosed by the invention, avian influenza viruses, chicken infectious bronchitis viruses, chicken Newcastle disease viruses, chicken infectious laryngotracheitis viruses, mycoplasma gallisepticum and mycoplasma synoviae can be accurately detected at the same time; the multi-fluorescent immunoassay method has high specificity, high sensitivity and good repeatability. Compared with a traditional detection method, the method disclosed by the invention realizes simultaneous detection of a plurality of types of different target molecules in the same sample; the use amount of the sample is less; the method is simple and rapid to operate and the detection cost can be greatly reduced.

The invention discloses a medicament for preventing and curing avian infectious laryngotracheitis and a preparation method thereof. The medicament is prepared by the following traditional Chinese herbs by weight: 12 parts of subprostrate sophora, 10 parts of scutellaria baicalensis, 12 parts of blackberry lilies, 10 parts of isatis roots, 8 parts of platycodon grandiflorum and 6 parts of liquorice. The preparation method of the medicament comprises the following steps: first extracting the herbs with ethanol to obtain extract; adding water to dregs of the decoction after ethanol extraction and extracting, and then concentrating and drying to obtain water extract powder; mixing with the extract and obtaining the needed medicament, or adding microcrystalline cellulose to serve as a filler and a joint disintegrating agent and making into dispersible tablets. The medicament for preventing and curing the avian infectious laryngotracheitis gives full consideration to anti-inflammation, resistance to secondary infection, medicament guidance to lung channels and other aspects, has considerably good curative effects, and also can improve absorption of the insoluble medicament, shorten the time of the medicament for taking effect and improve bioavailability of the insoluble medicament.

The invention discloses an infectious laryngotracheitis recombinant virus strain for expressing a Newcastle disease virus F protein and establishment and the application of the strain and relates to the field of recombinant virus vaccines. Aiming at problems of recombinant virus establishment and the application of an exogenous immunogenic gene with a nonobligatory gene US9 locus of an ILTV (Infectious Laryngotracheitis Virus), the invention provides an infectious laryngotracheitis recombinant virus strain which is named as rILTV US9-NDV-F, the microorganism preservation number of the strain is CGMCC No.14738, and the virus strain is an infectious laryngotracheitis recombinant virus which is established by replacing a US9 gene of the infectious laryngotracheitis recombinant virus by the Newcastle disease virus F protein, establishing an acquirement deficiency US9 gene and by inserting an NDV-F (Newcastle Disease Virus-F Protein) expression box into a corresponding position of the gene,and is used for recombining the Newcastle disease virus F protein. The recombinant infectious laryngotracheitis recombinant virus strain disclosed by the invention is used for preparing vaccines forpreventing infectious laryngotracheitis and other chicken infectious diseases.

The medicine for preventing and controlling chicken's infectious laryngotracheitis is prepared with ephedra, isatis root, Japanese felt fern frond, root of subprostrate sophora, airpotato yam and other 19 kinds of Chinese medicinal materials in certain weight proportion, and through drying, crushing, grinding and mixing. Before application, the medicine is soaked in boiled water for 30-60 min for the effective medicine components to be dissolved and leached fully and to be absorbed easily in chickens' bodies. At the same time, small amount of sugar and vitamin C as auxiliary medicines are added for raised curative effect. The medicine has high curative rate, no adverse reaction, no medicine residue, no drug resistance and other advantages.

The present invention provides a novel avian herpesvirus (NAHV) vector and recombinant vaccines made therefrom that are useful to immunize avian species against Marek's disease, infectious laryngotracheitis and Newcastle disease. Methods of immunizing an avian species against Marek's disease, infectious laryngotracheitis and Newcastle disease are also provided.

The invention discloses a veterinary drug for treating respiratory tract infection of poultry and a preparation method thereof. The veterinary drug is prepared mainly from the following materials by weight part: 30-120 parts of radix stemonae, 10-120 parts of scutellaria baicalensis, 30-210 parts of giant knot weed, 10-60 parts of indigo naturalis, 10-60 parts of artificial bezoar, 10-40 parts ofborneol, 10-90 parts of realgar, 10-60 parts of liquorice, 30-120 parts of platycodon root, 90-210 parts of cyrtomium fortunei, 30-120 parts of blackberry lily, 10-210 parts of dried toad, 150-420 parts of radix isatidis and 1-10 parts of bromhexine hydrochloride. The veterinary drug is prepared by grinding, screening and evenly mixing the materials, and grinding the materials by a superfine pulverizing technology into submicron powder and packing. The veterinary drug is mainly used for treating respiratory diseases of poultry caused by the bacterial viruses such as infectious bronchitis of chicken, infectious laryngotracheitis, newcastle disease and influenza. The invention has the advantages of rapid drug effect, favorable effect and little remaining charge, has simple preparation methodand is easy for promotion and application.

The invention discloses a gene chip and a kit for infectious bronchitis viruses and / or infectious laryngotracheitis viruses. The gene chip and detection kit disclosed by the invention can accurately and effectively detect infectious bronchitis viruses and / or infectious laryngotracheitis viruses, and are strong in specificity, high in sensitivity, short in time consuming and rapid in detection, and has a good application prospect.

The invention discloses veterinary medicine for treating animal infectious diseases, wherein the constituents inlude (by weight ratio) honeysuckle flower 33-39%, herba schizonepetae 16-25%, peppermint 16-25% and capsule of weeping forsythia 23-34%.

The invention discloses a traditional Chinese medicine for preventing and treating chicken infectious laryngotracheitis and a production method of the traditional Chinese medicine. The traditional Chinese medicine is prepared from the following raw material drugs according to a weight ratio: 100-400 of honeysuckle, 100-400 of radix scutellariae, 100-400 of tuber pinellia processed with ginger, 100-400 of dried orange peel, 100-400 of tuckahoe and 60-200 of licorice, wherein the honeysuckle, radix scutellariae, tuber pinellia processed with ginger, dried orange peel, tuckahoe and licorice are processed and extracted according to the amount so as to prepare into oral liquid, granulates, fluid extract, extract, powder, ultramicro powder and any pharmaceutical preparations capable of being clinically applied. The traditional Chinese medicine is significant in curative effect, is taken by drinking water or mixing with other materials, and is very convenient for transportation, storage and use.

The invention relates to a laying hen immune process. According to the specific procedures of the laying hen immune process, Marek vaccine is injected for laying hens which are 1 day old; ND-IB vaccine and triad vaccine are injected for laying hens which are 6 to 7 days old; bursa of fabricius vaccine is injected for laying hens which are 12 days old, bird flu vaccine H5 and henpox vaccine POX are injected for laying hens which are 17 days to 18 days old, bursa of fabricius vaccine is injected for laying hens which are 22 day old, ND-IB vaccine is injected for laying hens which are 26 days old, infectious rhinitis vaccine is injected for laying hens which are 32 days old, ND-IB vaccine and infectious laryngotracheitis vaccine are injected for laying hens which are 39 days to 40 days old, bird flu vaccine H5 is injected for laying hens which are 80 days old, ND-IB vaccine is injected for laying hens which are 90 days old, vaccine H9 and brain pox vaccine are injected for laying hens which are 110 days old, bird flue H5 vaccine is injected for laying hens which are 120 days old, and ND-IB vaccine, triad vaccine and insect expelling vaccine are injected for laying hens which are 130 days old. The laying hen immune process has the advantages that the laying hen diseases can be effectively controlled, and the health of the laying hens is ensured.

The invention relates to a traditional Chinese medicine feed additive for preventing and treating the infectious bronchitis and the anemopyretic cold of poultries, which is prepared from the followingselected raw medicinal materials by weight portion: 7 portions of ephedra herb, 6 portions of platycodon grandiflorum, 5 portions of subprostrate sophora, 6 portions of rabdosia rubescens, 4 portionsof scutellaria baicalensis, 8 portions of isatis roots, 5 portions of liquorice, 6 portions of fructus forsythiae, 6 portions of cordate houttuynia and 5 portions of radix Peucedani. A preparation method of the traditional Chinese medicine feed additive is realized as follows: the raw medicinal materials are mixed and then pulverized or respectively pulverized and then mixed. The selected raw medicinal materials have functions of clearing away heat and toxic material, dispelling wind and heat, reducing swelling and removing carbuncle and have good preventing and treating effect aiming at symptoms, such as snore, head swing, cough, snivel, strange squall, dyspnoea, depressed spirit, ingestion reduction, yellow and green excrement, and the like caused by the infectious bronchitis, the infectious laryngotracheitis, the anemopyretic cold, the rhinitis, the slow breathe, and the like of the poultries.

The invention discloses a traditional Chinese medicine for treating respiratory disease of livestock and a preparation method thereof. The traditional Chinese medicine comprises five traditional Chinese medicinal materials of herba violae, turkey corn, aster, herba ephedra and radix scutellariae. The traditional Chinese medicine can be effectively used for treating diseases of swine enzootic pneumonia, swine contagious pleuropneumonia, porcine reproductive and respiratory syndrome, swine plague, swine influenza, infectious bronchitis of chicken, infectious laryngotracheitis of chicken and thelike, and has the advantages of environment protection without toxic and side effects, simple preparation process, low production cost and the like.

The invention discloses a monoclonal antibody of chicken infectious laryngotracheitis virus gJ protein and application thereof. The monoclonal antibody is screened to obtain a hybridoma cell strain (3B1E6F6) which can stably secrete the anti-gJ protein monoclonal antibody, wherein the preservation number of the microbe is China general microbiological culture collection (CGMCC) No. 4337. The monoclonal antibody secreted by the hybridoma cell strain can be reacted with the ILTV WG (laryngotracheitis virus WG) virulent strain and can not be reacted with the other correlated pathogenies of the birds. The monoclonal antibody of the invention can be used for detecting the chicken infectious laryngotracheitis virus, and laying a foundation for building a fast, simple and exact diagnosis method and distinguishing the infectious laryngotracheitis wild toxicity infection from the recombinant vaccine immunity.

The invention belongs to the field of poultry culture and particularly discloses a method for raising fighting cocks. The method is characterized by carrying out culture according to the following steps: (1) hatching; (2) chickling breeding; (3) food adding management; (4) free-range raising; and (5) disease prevention: injecting baby chicks with vaccines monthly. According to the method, the probability of falling sick is cut off from source, edible feed for raising is added with an environment-friendly and safe traditional Chinese medicine mixture, and all traditional Chinese medicines are in synergism, so that avian infectious laryngotracheitis can be effectively prevented; in addition, the method is simple in operation, low in cost and easy to implement.