16 results about "Microorganism preservation" patented technology

The invention discloses bacillus velezensis B127 and application thereof, the bacillus velezensis is preserved in China Center for Type Microbiological Culture Collection, the preservation number is CCTCC NO: M 20252878, the preservation date is December 15, 2025, the classification name is bacillus velezensis B127, and the preservation address is Wuhan University, Hubei Province. The bacillus velezensis B127 has a remarkable inhibition effect on various tobacco fusarium pathogenic bacteria, has good phosphorus and potassium solubilizing capacity, generates hydrolase related to inhibition of pathogenic bacteria and generates siderophore and indoleacetic acid for promoting plant growth, is a biocontrol bacterium with excellent comprehensive performance, and can be widely applied to the field of biological control. The method has good development and application prospects in the fields of agricultural disease control, soil improvement, microbial fertilizers and the like.

The invention discloses a microorganism preservation solution, a preparation method, a microorganism sampling device and a use method, and belongs to the technical field of microorganism sampling and preservation. The microorganism preservation solution comprises the following components in parts by volume: 70-75 parts of a buffer solution with the pH value of 7.2-7.4, 5-10 parts of a protective agent, 0.1-0.65 part of an additive, 14-14.5 parts of a nutrient medium and 1-2 parts of an osmotic pressure regulator. The microorganism preserving fluid can ensure that microorganisms keep high activity after sampling, can effectively inhibit excessive proliferation of the microorganisms, avoids sample concentration distortion caused by too fast proliferation or inactivation, and provides reliable guarantee for accurate measurement of target microorganisms. The device comprises: a preservation container, which is provided with an opening and is filled with a microorganism preservation solution; the sampling cover detachably covers the opening in a sealing mode, a scraper and a brush head are installed on the bottom face of the sampling cover, bristles of the brush head are arranged around the scraper in the circumferential direction, and the brush head is located in the storage container and soaked in the microorganism storage liquid. And the sampling cross contamination or exposure risk is reduced.

The application discloses a freeze-drying protective agent for Streptococcus pneumoniae without animal source, and belongs to the field of microorganism preservation. The freeze-drying protective agent is composed of the following components: 1-5% (weight / volume) soybean peptone, 1-5% (weight / volume) trehalose, 4-8% (weight / volume) mannitol and 0.3-1.5% (volume / volume) glycerol, and the rest is water. The freeze-drying protective agent can better maintain the activity of Streptococcus pneumoniae, and the effect is equivalent to that of a skimmed milk protective agent or a commercially available freeze-drying protective agent without animal source, but the freeze-drying protective agent does not contain animal source components and has low cost.

The application provides a porcine epidemic diarrhea virus mutant strain and application thereof. The application obtains a porcine epidemic diarrhea virus mutant strain GX2506 from porcine intestinal tissue, and the strain is purified through passage, and the microorganism preservation number of the strain is CGMCC No. 47089. The isolated strain can stably proliferate on a passage cell, and typical cytopathic effect is generated. The porcine epidemic diarrhea virus mutant isolated strain has excellent immunogenicity; a vaccine prepared by using the isolated strain can induce piglets to generate high-level neutralizing antibodies; meanwhile, an ELISA kit prepared by using the isolated strain can better detect porcine epidemic diarrhea virus antibodies in serum.

The invention relates to the technical field of microorganism preservation, in particular to a novel gonococcus preservation solution which comprises the following components in parts by mass: 1-2 parts of a preservation agent, 40-60 parts of a solvent and 15-25 parts of a protective agent. According to the novel gonococcal preservation solution provided by the invention, the 30% glyceropancreatin soybean broth has a better gonococcal preservation effect than the conventional 10% skim milk at-20 DEG C, so that the gonococcal preservation of outpatient service and basic medical institutions with limited laboratory conditions is facilitated.

The invention belongs to the technical field of microorganism preservation, and particularly relates to normal-temperature preservation liquid for group B streptococcus (GBS) in a vaginal swab as well as a preparation method and application of the normal-temperature preservation liquid. The invention provides a GBS normal-temperature preservation solution. The formula of the GBS normal-temperature preservation solution comprises glycerol, glucose, sodium citrate, EDTA, FBS, compound amino acid, NaCl and the like. The preservation solution ensures that GBS can be stably preserved for 30 days at normal temperature, the activity of GBS and the stability of RNA are effectively maintained, the problems of RNA degradation and bacterial activity loss in an existing preservation method are solved, and reliable sample support is provided for subsequent bacterial culture and RNA detection. The method is suitable for long-time preservation and subsequent detection of clinical vaginal swab samples, and has a good application prospect.

The utility model provides a medical clinical microbiological detection sampling device and relates to the technical field of medical sampling. The medical clinical microbiological detection sampling device comprises a storage box, a heating wire is arranged in the storage box, a placement groove is formed in the storage box, a medicine bottle is arranged in the placement groove, a sealing cover is arranged at the upper end of the medicine bottle, a rubber cover is arranged in the sealing cover, and a sample conveying pipe is connected into the rubber cover. The side end of the sample conveying pipeline is connected with an air bag. According to the medical clinical microbiological detection sampling device, the medicine bottle and the heating wire are arranged, a specific microbiological preservation solution is injected into the medicine bottle, the heating wire is electrified to generate heat, heat is supplied to the medicine bottle, the human body temperature is simulated, and the temperature condition close to the original living environment of microorganisms can be provided for the microorganisms; the activity of the microorganisms can be maintained in the period from the time when the microorganisms are taken out of the human body to the time before detection, so that the detection result can more accurately reflect the real condition of the microorganisms in the human body.

The utility model relates to the technical field of microorganism preservation, in particular to a strain preservation tube for a microorganism laboratory, which comprises a tube body, a plurality of porcelain beads and a sealing component, and the sealing component comprises a sealing cover, a sealing piece, a threaded cap, an external threaded ring, two butt joint pieces, an assembly ring and two auxiliary limiting structures. The thread cap is twisted to be in threaded connection with the external thread ring on the pipe body, the sealing piece on the sealing cover is driven to be inserted into the inner wall of the pipe body to seal the pipe body, the two butt-joint pieces are inserted into the limiting frames on the two sides of the pipe body correspondingly, and after the butt-joint pieces are inserted, the fastening pieces are operated to conduct auxiliary fixing on the butt-joint pieces. According to the strain preservation tube, the sealing cover is arranged on the tube body, so that the tightness of closing of the sealing cover and the tube body is improved, and the problem that the sealing effect on the tube body is affected due to the fact that the top cover and the tube body are closed and sealed in a rotating mode, and errors occur in the operation process, so that the top cover rotates, the tube body is loosened and the sealing effect on the tube body is affected is solved.

The invention relates to a method for preparing natural not-from-concentrate olive juice from olives or olive by-products such as two-phase olive pomace (alpeorujo), wherein the method comprises: (a) separating the natural olive juice using mechanical processes, at less than 50°C, without adding extra water, preferably wherein the mechanical processes selected from the group consisting of centrifuging and / or filtering; and (b) preserving the natural olive juice obtained in step (a) by means of a thermal and / or non-thermal treatment to inactivate enzymes and / or microorganisms. The invention also relates to a method for preparing a beverage of functional food, wherein the method comprises mixing the natural olive juice with a base beverage or food. The base beverage may be a fruit juice or a vegetable juice.

The invention belongs to the technical field of microorganism preservation, and particularly relates to 10 CFU mycoplasma standard strain stationary liquid applicable to sensitivity verification of a mycoplasma rapid detection method. The stationary liquid comprises a basal culture medium, a low-temperature protective agent and additive components, wherein the additive components comprise ethanolamine, insulin, transferrin, human serum albumin, vitamin C, a cell membrane stabilizer and a nuclease inhibitor. By using the stationary liquid provided by the invention, 10 CFU mycoplasma standard strains can be preserved for 15 months at-20 DEG C, the integrity of the mycoplasma cell structure can be basically maintained, and the mycoplasma nucleic acid can be stably preserved; in addition, the mycoplasma standard strain can tolerate thermal change and freeze-thaw change at 37 DEG C, and has good thermal stability and freeze-thaw stability, so that the technical problems of harsh transportation and storage conditions, short preservation time and poor stability of the 10 CFU mycoplasma standard strain are solved.

The application discloses a porcine epidemic diarrhea virus G2b subtype strain and application thereof. The application obtains a porcine epidemic diarrhea virus G2b subtype GZ2504 strain from a porcine intestinal tissue, and the strain is purified through passage, and the microorganism preservation number of the strain is CGMCC No. 47083. The isolated strain can stably proliferate on a passage cell and produce a typical cytopathic effect. The porcine epidemic diarrhea virus G2b subtype isolated strain has excellent immunogenicity; a vaccine prepared by using the isolated strain can induce piglets to produce high-level neutralizing antibodies; meanwhile, an ELISA kit prepared by using the isolated strain can better detect porcine epidemic diarrhea virus antibodies in serum.

The invention discloses a biofilm state probiotic microcapsule and application thereof in improving the tolerance of probiotics, and belongs to the technical field of microorganism preservation and maintenance. The biofilm state probiotic microcapsule is prepared by the following method: mixing a probiotic bacterial suspension with a polysaccharide solution, then contacting the mixed solution with a cross-linking agent solution, and carrying out cross-linking to obtain a polysaccharide cross-linked gel microcapsule, adjusting the pH value of the complex culture medium to 5-9, then adding the polysaccharide cross-linked gel microcapsule, and culturing to obtain the biofilm state probiotic microcapsule. The microcapsule has excellent tolerance to gastric juice and cholate, so that the problem of low survival rate of probiotics in a gastrointestinal tract environment is solved.

The application belongs to water pollution treatment technology, and particularly relates to an anaerobic dehalogenation microbial preservation method and application. The dehalogenation microorganism is resuspended in a embedding agent solution, and then the resuspension is added dropwise into a crosslinking agent, and then is reacted in an ice bath under anaerobic conditions for 10-20 min; after the reaction, the crosslinking is continued for 24-36 h under anaerobic conditions at 4-6 DEG C, and the preservation of the anaerobic dehalogenation microorganism is realized after the crosslinking. The anaerobic dehalogenation microorganism is fixed, and through centrifugation, resuspension and crosslinking into a ball under strict anaerobic conditions, the immobilized bacterial agent is obtained while the bacterial solution is concentrated and the cells are wrapped, the tolerance of the dehalogenation microorganism to oxygen is significantly improved, the problem that the dehalogenation microorganism is easy to be exposed to air and lose the degradation activity in the form of culture solution is overcome, and great convenience is provided for the implementation of in-situ bioremediation engineering of a contaminated site.

The application discloses bacillus safensis with weed seed germination inhibition function, compost prepared therefrom and application. The application screens a strain NG-50 which can efficiently degrade cellulose, has excellent nitrogen fixation function and good stress resistance function, can resist drought stress, saline-alkali stress, acid stress and high temperature stress by isolating microorganisms in soil after straw field return in desert edge. The strain NG-50 is identified as bacillus safensis by molecular biology, and its microorganism preservation number is CGMCC No. 35363. It is proved by tests that the strain NG-50 can effectively inhibit weed seed germination and significantly promote crop growth. Application of the strain NG-50 in preparation of agricultural and forestry waste compost can significantly shorten the fermentation period of the compost and obviously improve the compost quality, and the strain NG-50 has potential as a composting agent for agricultural and forestry waste.

The present invention belongs to the technical field of microorganism preservation, and relates to a microorganism cryopreservation solution and a preservation method thereof. The present invention provides a microorganism cryopreservation solution, which is composed of a protective solution and a carrier; the protective solution is composed of skim milk powder, β-cyclodextrin, glycerol and glucose; the carrier is a porous foam metal material modified by acrylic acid. The present invention also provides a preservation method for microorganisms, which can achieve conventional preservation for 30 days at -5 to 0 °C, cryopreservation for 6 months at -20 to -18 °C, and vacuum freeze-drying preservation for 12 months at -90 to -80 °C.

A soft boiled egg sterilization system and method relates to the technical field of food processing, and comprises a steam preheating device, a high temperature sterilization device, a cooling device and a sterilization tank; the steam preheating device, the high-temperature sterilization device and the cooling device are all connected with the sterilization tank, and the steam preheating device is used for introducing high-temperature steam into the sterilization tank; the high-temperature sterilization device is used for introducing high-temperature hot water into the sterilization tank after preheating; the cooling device is used for introducing cooling water into the sterilization tank after high-temperature sterilization. According to the method, commercial sterility can be guaranteed, meanwhile, the semi-liquid state of the yolk is kept through the top-speed temperature-rising temperature-controlling and rapid-cooling technology, the soft core character of the yolk can be kept, microorganisms on the surface of the yolk can be killed, the preservation time is long, and the taste is good.