4 results about "Microorganism preservation" patented technology

The application provides a porcine epidemic diarrhea virus mutant strain and application thereof. The application obtains a porcine epidemic diarrhea virus mutant strain GX2506 from porcine intestinal tissue, and the strain is purified through passage, and the microorganism preservation number of the strain is CGMCC No. 47089. The isolated strain can stably proliferate on a passage cell, and typical cytopathic effect is generated. The porcine epidemic diarrhea virus mutant isolated strain has excellent immunogenicity; a vaccine prepared by using the isolated strain can induce piglets to generate high-level neutralizing antibodies; meanwhile, an ELISA kit prepared by using the isolated strain can better detect porcine epidemic diarrhea virus antibodies in serum.

The application discloses a porcine epidemic diarrhea virus G2b subtype strain and application thereof. The application obtains a porcine epidemic diarrhea virus G2b subtype GZ2504 strain from a porcine intestinal tissue, and the strain is purified through passage, and the microorganism preservation number of the strain is CGMCC No. 47083. The isolated strain can stably proliferate on a passage cell and produce a typical cytopathic effect. The porcine epidemic diarrhea virus G2b subtype isolated strain has excellent immunogenicity; a vaccine prepared by using the isolated strain can induce piglets to produce high-level neutralizing antibodies; meanwhile, an ELISA kit prepared by using the isolated strain can better detect porcine epidemic diarrhea virus antibodies in serum.

The application belongs to water pollution treatment technology, and particularly relates to an anaerobic dehalogenation microbial preservation method and application. The dehalogenation microorganism is resuspended in a embedding agent solution, and then the resuspension is added dropwise into a crosslinking agent, and then is reacted in an ice bath under anaerobic conditions for 10-20 min; after the reaction, the crosslinking is continued for 24-36 h under anaerobic conditions at 4-6 DEG C, and the preservation of the anaerobic dehalogenation microorganism is realized after the crosslinking. The anaerobic dehalogenation microorganism is fixed, and through centrifugation, resuspension and crosslinking into a ball under strict anaerobic conditions, the immobilized bacterial agent is obtained while the bacterial solution is concentrated and the cells are wrapped, the tolerance of the dehalogenation microorganism to oxygen is significantly improved, the problem that the dehalogenation microorganism is easy to be exposed to air and lose the degradation activity in the form of culture solution is overcome, and great convenience is provided for the implementation of in-situ bioremediation engineering of a contaminated site.

The application discloses bacillus safensis with weed seed germination inhibition function, compost prepared therefrom and application. The application screens a strain NG-50 which can efficiently degrade cellulose, has excellent nitrogen fixation function and good stress resistance function, can resist drought stress, saline-alkali stress, acid stress and high temperature stress by isolating microorganisms in soil after straw field return in desert edge. The strain NG-50 is identified as bacillus safensis by molecular biology, and its microorganism preservation number is CGMCC No. 35363. It is proved by tests that the strain NG-50 can effectively inhibit weed seed germination and significantly promote crop growth. Application of the strain NG-50 in preparation of agricultural and forestry waste compost can significantly shorten the fermentation period of the compost and obviously improve the compost quality, and the strain NG-50 has potential as a composting agent for agricultural and forestry waste.