49 results about "Bacillus safensis" patented technology

The invention relates to the technical field of microorganisms, and particularly discloses a saline-alkaline tolerant bacillus deserticola BW-13 with biocontrol and growth promoting functions and application thereof. The bacillus safensis BW-13 is preserved in the China Center for Type Culture Collection on January 13, 2025, the classification name of the bacillus safensis BW-13 is bacillus safeensis, and the preservation number of the bacillus safensis BW-13 is CCTCC NO: M 2025108. The strain BW-13 has relatively high salt tolerance, and still keeps good resistance under various stress conditions; meanwhile, the plant growth promoting agent has various plant growth promoting characteristics including nitrogen fixation, phosphorus solubilization and cellulase and protease secretion capacity, and pot experiment verifies that the plant growth promoting agent can improve the salt resistance of cucumber seedlings and promote plant growth; the strain has an antagonistic effect on a plurality of phytopathogens, and especially has a remarkable prevention and treatment effect on plant diseases such as apple ring spot, polygonatum kingianum anthracnose, pear tree rot and tomato gray mold caused by ascomycophyta pathogenic fungi.

The invention provides a complex microbial inoculant as well as a preparation method and application thereof, and relates to the technical field of complex microbial inoculant preparation. The compound bacterial agent is prepared from bacillus pumilus JMB004, bacillus safeensis strain BXC22 and bacillus sp. EB35, and the compound bacterial agent is prepared from bacillus pumilus strain JMB004, bacillus safeensis strain BXC22 and bacillus sp. EB35. The three strains are obtained through autonomous screening and prepared into the complex microbial inoculant, dry powder can be stored at normal temperature, the storage life is prolonged to one year, and the composite microbial inoculant is convenient to carry. The complex microbial inoculant is only composed of three strains of bacteria, and the components are simple; the low-temperature composting agent is applied to composting treatment under a low-temperature condition, and is relatively high in temperature rise rate, relatively short in fermentation time and relatively high in decomposition degree.

The invention provides a complex microbial inoculant and application of the complex microbial inoculant in prevention and treatment of tobacco root rot, and belongs to the technical field of biocontrol inoculants. The biocontrol microbial inoculum disclosed by the invention is prepared by mixing and fermenting bacillus megatherium T482 and bacillus safensis YN-LJG-4. According to the invention, the two bacteria are subjected to co-fermentation, such that the composite bacterial agent is prepared. The bacillus megaterium T482 and the bacillus safensis YN-LJG-4 have a synergistic effect, so that the tobacco root rot can be effectively prevented and treated, and the tobacco quality can be improved. The complex microbial inoculant provided by the invention is simple in preparation method, has multiple effects, and shows a good application prospect in prevention and treatment of tobacco rhizome diseases as a biocontrol inoculant.

The invention relates to the technical field of microorganisms, in particular to bacillus licheniformis JSF-9 and a complex microbial inoculant and application thereof.The strain JSF-9 is obtained by being separated from healthy cattle rectum contents by a research group, and it is detected that the strain has the good beta-1, 4-glucanase producing capacity, and the beta-1, 4-glucanase producing capacity is high. The antibacterial effect on staphylococcus aureus, salmonella typhimurium and shigella flexneri is achieved; the strain has a good bagasse fermentation capability, for example, after the strain, bacillus altitudinis MLF-1 and bacillus safensis MLL-5 are prepared into a mixed microbial inoculum, the bagasse fermentation capability is better, and verification shows that the complex microbial inoculum can effectively inhibit the reproduction of harmful bacteria, so that the preservation period of fermented feed is prolonged, and the yield of the fermented feed is improved. Animal feeding experiments find that the nutritional value and the disease resistance of the complex microbial inoculant fermented bagasse are remarkably improved, 10-15% of the complex microbial inoculant fermented bagasse is added into basic ration, and the cost-reducing and effect-increasing effects are achieved for feeding Jersey cattle.

The invention discloses a bacillus safensis strain and application thereof in aquaculture, and relates to the technical field of aquaculture disease prevention and control, the obtained bacillus safensis strain ZY-1 forms spores under a certain fermentation condition, can be prepared into powder for preservation, has a long shelf life, has the bacteriostatic activity retention rate of more than or equal to 85% after being stored in a dark place for 12 months at 4-25 DEG C, and has the antibacterial activity of more than or equal to 7.2%. The production, transportation and storage cost is saved, main bacteriostatic effect factors of the bacillus safensis ZY-1 strain exist in extracellular metabolites, the bacillus safensis ZY-1 strain is stable in performance and resistant to high temperature, acid and alkali, ultraviolet rays, protease and the like, and fermentation supernatant of the bacillus safensis ZY-1 strain can be directly applied or prepared into spray-dried powder and is suitable for various aquaculture application scenes such as pond splashing and feed mixing and feeding. The metabolite spray-dried powder has a remarkable killing effect on high-pathogenicity vibrios VpAHPND and VpTPD, the minimum inhibitory concentrations of metabolite spray-dried powder respectively reach 23.44-46.88 mu g / ml and 46.88-93.75 mu g / ml, and the metabolite spray-dried powder can be used for preventing and controlling prawn infectious hepatopancreas necrosis and prawn transparent larva diseases induced by the metabolite spray-dried powder and also has a certain killing function on pathogens of other aquatic animals.

The invention discloses bacillus safensis and application thereof in prevention and control of pine wood nematode diseases, and belongs to the technical field of biocontrol of plant diseases. The invention discloses bacillus safensis GDWS1 which is separated from resistant masson pine tissues and has a relatively good inhibition effect on pine wood nematode diseases, and the preservation number of the bacillus safensis GDWS1 is GDMCC No: 66264. The strain GDWS1 has high lethal activity on pine wood nematodes, the correction death rate of the strain GDWS1 on the pine wood nematodes reaches 90% or above, and active substances capable of poisoning and killing the pine wood nematodes and good in stability can be generated; the requirements on culture conditions are low, and the ecological environment is friendly and pollution-free. A greenhouse control experiment is carried out by using pinus massoniana seedlings, the strain GDWS1 can effectively reduce the disease index of the pinus massoniana seedlings inoculated with pine wood nematodes, the control effect reaches 45.88%, and the strain has a good application prospect in prevention and control of pine wood nematode diseases, provides a new biocontrol bacterium type for biological prevention and control of pine wood nematode diseases, and has the potential of being developed into biopesticides.

The invention relates to bacillus safensis AHAU-WU410 and application of the bacillus safensis AHAU-WU410 in prevention and treatment of corn bacterial wilt and promotion of corn plant growth, and belongs to the technical field of biological prevention and treatment, a living body pure culture of the bacillus safensis AHAU-WU410 is preserved in China Center for Type Culture Collection in Wuhan University, the preservation date is August 4, 2025, the preservation number is CCTCC NO: M 20251769, and the preservation number is CCTCC NO: M 20251769. The bacillus safensis AHAU-WU410 has a remarkable antagonistic effect on pathogenic bacteria pantoea stutzeri of the corn bacterial wilt, and can promote the growth of corn plants by secreting IAA (Indoleacetic Acid) and playing a phosphate solubilizing role. The bacillus safensis AHAU-WU410 is screened and identified, the control effect, the biocontrol mechanism and the growth promoting function of the biocontrol bacterium are verified and analyzed, and a new direction and a theoretical basis are provided for developing a green biocontrol bacterium agent related to corn bacterial disease control.

The invention discloses a composite biocontrol bacterium for preventing and treating tobacco bacterial wilt and application of the composite biocontrol bacterium. Belongs to the technical field of biological control. The compound biocontrol bacteria comprise pseudomonas putida YZ03102604, bacillus safensis YZ03102605 and bacillus velezensis YZ23120529, and the compound biocontrol bacteria comprise pseudomonas putida YZ03102604, bacillus safensis YZ03102605 and bacillus velezensis YZ23120529. The composite biocontrol bacterium can be prepared into a microbial inoculum with auxiliary materials, and the microbial inoculum is applied during tobacco transplanting and after tobacco transplanting. Potting and field experiment results show that the composite biocontrol bacterium can significantly reduce the morbidity and disease index of bacterial wilt, the prevention and treatment effect reaches 76% or above, and the composite biocontrol bacterium is superior to a single strain and other combinations and has a good biocontrol application prospect.

The invention relates to a mild thermal enhanced photodynamic spore inactivation method, which combines a photodynamic technology with 65 DEG C mild thermal treatment, and is used for killing bacillus altitudinis spores and bacillus safensis spores in a germination stage. The method disclosed by the invention has an excellent killing effect on spores of bacillus altitudinis and spores of bacillus safensis, can realize efficient inactivation of the spores in meat products, solves the defects of the existing photodynamic technology for inactivating the spores, adopts a sterilization temperature far lower than a commercial sterilization temperature, can better maintain the flavor and quality of foods, and is suitable for industrial production. And the method has universality in food industry application.

This invention discloses a strain of *Bacillus saeformis* H1, its culture method, and its applications, relating to the field of microbial technology. The strain was deposited on March 31, 2026, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 38117. This strain exhibits strong environmental adaptability, can stably grow under neutral to strongly alkaline conditions, and secretes antibacterial substances with low toxicity and no pathogenicity. After optimization, its optimal liquid fermentation conditions are: 5 g / L yeast extract, 10 g / L peptone, 37 g / L sucrose, pH=10, temperature 25℃, rotation speed 140 rpm, and culture time 24 h. The fermentation supernatant of this strain shows significant inhibitory effects on Gram-positive bacteria such as *Staphylococcus aureus*, methicillin-resistant *Staphylococcus aureus*, and *Streptococcus pneumoniae*, and can be prepared into drug sensitivity tablets. The *Bacillus saeformis* H1 provided by this invention can be used to prepare antibacterial drugs or antibacterial infection drugs, providing a new strategy for alleviating the problem of antibiotic resistance.

The invention relates to the technical field of microorganisms, in particular to a beta-1, 4-endo-xylanase gene BsxynA and application of the beta-1, 4-endo-xylanase gene BsxynA. According to the application, the beta-1, 4-endo-xylanase gene BsxynA is screened and identified, in-vitro induced expression is performed on the beta-1, 4-endo-xylanase gene BsxynA for the first time, and enzymatic properties of the beta-1, 4-endo-xylanase gene BsxynA are researched The enzyme activity of the bacillus safensis xylanase reaches 83.913 U / mL, the bacillus safensis xylanase is expected to be subjected to gene mutation through mutation breeding such as physical (such as ultraviolet rays and gamma rays) or chemical (such as nitrosoguanidine and diethyl sulfate) mutagenic agent treatment, and bacillus safensis mutants with higher enzyme activity are directionally screened. For example, ultraviolet mutagenesis can cause random mutation of the DNA of the microorganism, change the expression of the gene or the structure of the zymoprotein, and possibly increase the xylanase yield or enhance the xylanase activity. The produced BsXynA mainly promotes the hydrolysis of beech xylan, so that xylobiose (X2) and xylotetraose (X4) are generated, and xylose is not generated. In addition, the BsXynA significantly improves the digestive performance of the animal feed, and can increase the xylose content in the feed, promote the nutrient absorption of animals and reduce the production cost.

The application discloses Bacillus safensis HNXJ-B1 and application thereof, is preserved in China Microorganism Strain Preservation Management Committee general microorganism center, and its preservation number is: CGMCC NO: 32527, and the preservation date is November 7, 2024.The application is identified by 16S rDNA strain, and it is confirmed that HNXJ-B1 is Bacillus safensis.The inhibition effect on Ralstonia solanacearum, Diaporthe sclerotioides, Trichophyton mentagrophytes and Malassezia is obvious, can simultaneously inhibit plant disease pathogenic bacteria and animal pathogenic fungi, and has wide application prospect.

The invention relates to the field of biocontrol of plant diseases. The invention provides a bacillus safensis strain HMQAU23223, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 13, 2025, and the preservation registration number is CGMCC No.36623. The bacillus safensis strain HMQAU23223 has the advantages that the preservation number is CGMCC No.36623; the invention also provides a biocontrol inoculant. The invention also provides a preparation method of the biocontrol inoculant. The invention further provides the application of the bacillus safensis strain HMQAU23223 or the biocontrol microbial inoculum or the biocontrol microbial inoculum prepared by the preparation method. The invention further provides the application of the bacillus safensis strain HMQAU23223 or the biocontrol microbial inoculum prepared by the preparation method. The invention further provides a method for preventing and treating plant diseases. The invention also provides an application of the bacillus safensis strain HMQAU23223 in preparation of a compound for inhibiting phytopathogen or preventing and treating plant pathogenic nematodes. The fermentation filtrate of the bacillus safensis strain HMQAU23223 provided by the invention has the lethal effects of 89% or above on pine wood nematode, meloidogyne incognita, wheat cyst nematode, heterodera filipjevi and ditylenchus destructor, and has better biocontrol potential.

This invention relates to the field of microbial technology, specifically to a strain of *Bacillus safranin* and its application in the preparation of flocculants. The strain is *Bacillus safranin*. (Bacillu s safensis)BS‑RCEES‑ 2025‑001 The strain was deposited at the China General Microbiological Culture Collection Center on December 30, 2025, with accession number [accession number missing]. CGMCCNo.37242。 The present invention is based on Bacillus sarfus. (Bacillus safensis)BS‑RCEES‑2025‑001 The strain secretes extracellular polysaccharides that have a strong flocculation effect, and the flocculation rate of the wastewater treated with the flocculant prepared from it is 80% to 94%.

The invention discloses a Bacillus saffors-Bacillus atrophaeus compound bacterium and application thereof, the compound bacterium comprises Bacillus saffors BM-7 and Bacillus atrophaeus QB-2-7, the Bacillus saffors BM-7 is preserved in Guangdong Microbial Culture Collection Center on January 13, 2025, the preservation number is GDMCC NO: 65770, the preservation number is CGMCC NO: 65770, the preservation number is CGMCC NO: 65770, and the preservation number is CGMCC NO: 65770. The bacillus atrophaeus QB-2-7 is preserved in Guangdong Microbial Culture Collection Center on January 13, 2025, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 65771. The compound bacterium does not have hemolytic activity, is high in safety, has a good inhibition effect on colletotrichum gloeosporioides, has stable physicochemical properties, relatively strong biofilm forming ability, temperature tolerance and illumination and ultraviolet tolerance, is relatively wide in pH value application range, and has the potential of being applied to field disease control.

The invention discloses a strain of bacillus safensis and application thereof, the bacillus safensis ZHT-9 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M20241011, and the preservation address is Wuhan University, Wuhan, China. The bacillus safensis ZHT-9 disclosed by the invention has a remarkable blood fat reducing function in animal body blood, and shows a unique phenomenon that a mouse is not fat after eating too much in a mouse model.

The invention belongs to the field of tobacco treatment, and particularly relates to an enzyme composition which comprises a first enzyme obtained by fermenting bacillus subtilis with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.24244; and one or two of the following items: a second enzyme obtained by fermenting bacillus safensis with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.24246; the bacillus subtilis with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.24134 is fermented to obtain the third enzyme. The invention also relates to a method for extracting the aroma component from the tobacco waste, an extract containing the aroma component, a tobacco composition and a tobacco product. According to the enzyme composition, the extraction rate of aroma components of the tobacco waste can be remarkably increased; and / or the extract is added into the cigarette, so that the tobacco fragrance and the fresh and sweet fragrance of the cigarette can be increased during smoking, the soft feeling and the roundness of smoke are increased, the aftertaste comfort degree and the fragrance harmony are improved, the offensive odor and the irritation are reduced, and the overall sensory quality is remarkably improved.

The invention discloses a tea seed cake processing method and application thereof, and relates to the field of tea seed cake processing.The tea seed cake processing method comprises the following steps that a microorganism compound seed solution is obtained, and microorganisms comprise bacillus safensis and bacillus licheniformis; inoculating the microbial composite seed liquid into a tea seed cake solid culture medium, and performing solid fermentation to obtain a fermentation product; performing primary purification treatment on the fermentation product to separate out tea saponin and a first mixture; performing secondary purification treatment on the first mixture to separate out tea polysaccharide and a second mixture; and drying the second mixture to obtain the high-protein feed. Through microbial fermentation, non-protein substances in the tea seed meal are converted into protein, the protein content in the tea seed meal is increased, tea saponin and tea polysaccharide in the tea seed meal are extracted, the utilization rate of the tea seed meal is increased, the conversion rate of the tea saponin and the tea polysaccharide is increased, and high-value utilization of the tea seed meal is achieved.

The invention discloses bacillus safensis with high lipase activity and application of the bacillus safensis in degradation of biodegradable mulching films and growth promotion of crops, and belongs to the technical field of agricultural microorganisms and biodegradable materials. According to the present invention, the bacterial strain is bacillus safeensis STX-S1, and is preserved in the China Center for Type Culture Collection on September 15, 2025, and the preservation number is CCTCC NO: M 20252036; the lipase can be stably secreted under neutral to weakly acidic and weakly alkaline conditions; a PBAT or PLA film can be efficiently degraded, the biodegradable mulching film is degraded, and the growth of crops is promoted.

This invention discloses an organic fertilizer for increasing guava yield in saline-alkali land and its preparation method, relating to the field of biotechnology. The active ingredients of this organic fertilizer include *Bacillus horikoshii* ACCC 01061, *Bacillus safensis* CICC 21743, *Sporidiobolus salmonicolor* CICC 32895, *Trichoderma koningii* CICC 13006, and *Trichoderma longibrachiatum* ACCC 32095. This organic fertilizer can improve the yield per guava tree and the quality of the fruit grown in saline-alkali land, and has significant market application value.

The invention relates to the technical field of microorganisms, in particular to bacillus altitudinis MLF-1 and a complex microbial inoculant and application thereof.The bacillus altitudinis strain MLF-1 is obtained by being separated from healthy cattle rectum contents by a research group, and it is detected that the strain has the good beta-1, 4-glucanase producing capacity, and the beta-1, 4-glucanase producing capacity is high; the antibacterial effect is achieved on enterobacter aerogenes, staphylococcus aureus, salmonella typhimurium and escherichia coli; the bacillus altitudinis MLF-1 has good fermentation capacity on the siraitia grosvenorii residues, after the bacillus altitudinis MLF-1 is mixed with the bacillus licheniformis strain JSF-9 and the bacillus safensis strain MLL-5 to prepare the complex microbial inoculant, the nutritional value and the disease resistance of the siraitia grosvenorii residues fermented by the complex microbial inoculant are remarkably improved, 10%-15% of the complex microbial inoculant is added into a basic ration, and the content of the complex microbial inoculant in the siraitia grosvenorii residues fermented by the complex microbial inoculant is reduced. The Jersey cattle feed has the effects of reducing cost and improving efficiency for feeding Jersey cattle.

The invention belongs to the technical field of agricultural probiotic screening application, and particularly relates to a radiation-resistant bacterium and application thereof. The radiation-resistant bacterium is bacillus safensis, and the preservation number of the bacillus safensis is CCTCC (China Center for Type Culture Collection) NO: M 20251993. Radiation-resistant microorganisms are applied to the fields of saline-alkali soil improvement and stress agricultural production, the traditional cognition that the microorganisms are mainly limited to the fields of basic research or radiation protection for a long time is broken through, and important expansion of application scenes of the microorganisms is achieved. Under the adverse conditions of salt, alkali and the like, although the growth of the radiation-resistant strain R1 in the initial stage is possibly inhibited, the physiological activity can be rapidly adjusted and recovered, and the stable growth and metabolic function can be maintained. The germination rate of crop seeds treated by the strain is remarkably improved in salt stress and alkali stress environments, and robust growth of germs and radicles can be effectively promoted. In addition, the Bacillus safensis strain is a common safe microorganism in the environment, has excellent environmental compatibility, and has a safety basis and a practical prospect for large-scale popularization and application.

The invention provides a potato late blight biocontrol strain as well as a biocontrol inoculant and application thereof, and belongs to the technical field of biocontrol bacteria. The invention discloses a bacillus safeensis strain B1, and the biological preservation number of the bacillus safeensis strain B1 is CGMCC (China General Microbiological Culture Collection Center) No.36024. The invention also provides a biocontrol bacterium composition which is prepared by compounding the Leifsonia sp. Strain L1 alone or by combining the Leifsonia sp. Strain L1 with the Leifsonia sp. Strain L6 and the strain B1. The strain B1 has good activity of inhibiting phytophthora infestans, meanwhile, the biocontrol bacterium composition not only further improves the antibacterial activity of the strain B1, but also shows that the biocontrol bacterium composition can effectively reduce the disease index of potatoes, and compared with the single-phase strain B1, the biocontrol bacterium composition can effectively improve the prevention effect of potato late blight.

The application belongs to the field of agricultural microbial technology, and particularly relates to a plant endophytic Bacillus safensis and application thereof in plant disease prevention and treatment. The plant endophytic Bacillus safensis has a strain number of BSA15, a classification name of Bacillus safensis Bacillus safensis , a preservation number of CGMCC No. 36637, and is preserved in the China General Microbiological Culture Collection Center on November 17, 2025. The Bacillus safensis has a broad-spectrum inhibitory activity on pathogenic fungi and oomycetes, and can produce a significant inhibitory effect on plant pathogenic microorganisms through multiple mechanisms. In addition, the Bacillus safensis BSA15 can be stably colonized in the plant body as an endophyte, further enhancing the disease prevention and control effect. Using the strain for disease prevention and treatment can significantly reduce the use amount of chemical pesticides, reduce pesticide residues and environmental pollution, and meet the requirements of green agriculture and sustainable development.

The invention provides a bacillus safensis Sys-Gs-001 strain for producing ginsenoside CK, the strain is preserved in the China Center for Type Culture Collection on November 24, 2025, the preservation number is CCTCC NO: M20252681, the preservation number is CCTCC NO: M20252681, the preservation number is CCTCC NO: M20252681, and the preservation number is CCTCC NO: M20252681. The 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the strain is as shown in SEQ ID NO. 1. The bacillus safensis Sys-Gs-001 strain provided by the invention can produce ginsenoside CK through metabolism, can be subjected to large-scale fermentation culture in a short time, is relatively low in fermentation cost, is not limited by conditions such as time domains and seasons, and has remarkable advantages. Meanwhile, the invention also provides optimized microbial fermentation conditions and a formula of the culture medium, and the yield of the ginsenoside CK is relatively high.

This invention relates to the field of biotechnology, and particularly to a strain of Bacillus salafulae subsp. salafulae and its applications. This invention uses a strain of Bacillus salafulae subsp. salafulae as the core functional bacterium to construct a solid / semi-solid fermentation process window that can operate stably at high temperatures of 30-55℃. Through "high-temperature steady-state enzyme production and hydrolysis + process temperature and humidity control and impurity suppression + optional compatibility for synergistic nitrogen locking / adsorption," low ammonia odor and nutrient release are achieved, resulting in fermented feed products with higher nutrient availability, milder odor, and better batch consistency.

The present application is a method for effectively inactivating spores in Bacillus by using chelating resin in the field of sterilization. The method uses chelating resin to inactivate spores in Bacillus. Specifically, chelating resin is used to quickly and effectively inactivate spores in Bacillus cereus, Bacillus safensis, Bacillus albus, and Bacillus subtilis. The chelating resin used has low production cost and can be regenerated and reused, achieving effective inactivation of spores in a simple, low-cost, and environmentally friendly manner.

Disclosed are novel bioremediation formulations and inocula for formulation of bioremediation formulations. The formulations of the present disclosure comprise viable microorganisms of the species Paenibacillus xylanexedens, Pseudomonas fluorescens, Bacillus safensis, Streptomyces griseus, Micrococcus luteus, and Bacillus megaterium and can be used to degrade glyphosate residue associated with a substrate contaminated with glyphosate, and thereby remedially treat the substrate. Related methods are also disclosed.

The present invention discloses a Bacillus safensis GX-H6 strain and its application. The Bacillus safensis GX-H6 strain was deposited in the General Microbiology Center of the China National Committee for the Preservation of Microorganisms on June 16, 2022, with a deposit number of CGMCC No. 25106. The Bacillus safensis GX-H6 of the present invention has a very good antagonistic effect on the pathogens of rice bacterial leaf streak, rice bacterial leaf blight, citrus canker and various plant pathogenic fungi. The strain and its fermentation liquid can be used to prepare biocontrol agents, are easy to industrialize, have low production costs, have good market prospects, and also have important industrial application value.

The invention relates to a complex microbial inoculant for preventing and treating tobacco bacterial wilt and application of the complex microbial inoculant. Strain in the complex microbial inoculant comprises a mulberry enterobacter 65D6 strain, a Ralstonia 56D2 strain, an agrobacterium 18A6 strain, an arthrobacter uretogenes 05-507 strain and a bacillus safensis 05-2101 strain. The five specific strains are compounded and combined for use, an excellent tobacco bacterial wilt prevention and treatment effect is achieved, and experimental research shows that the disease occurrence of tobacco plants treated by the complex microbial inoculant is obviously slowed down, and the disease index is obviously lower than that of a clear water control group and that of a thiediazole copper and chloriure.copper sulfate chemical agent control group. Meanwhile, the five strains can be mutually matched and promoted, a synergistic effect is achieved in the aspect of preventing and treating tobacco bacterial wilt, the WY11 strain is further added for combined use, the effect of preventing and treating the tobacco bacterial wilt is improved, and the better effect of promoting the growth of tobacco plants can be achieved.