61 results about "Xylobiose" patented technology

The invention relates to a method for preparing xylo-oligosaccharide which utilizes and extrudes auxiliary enzymolysis wheat bran, in specific belongs to the technology field of agricultural and sideline product development, functional food additive and nutrition healthcare. With the method, wheat bran is taken as the main raw material and the bran is crushed and enzymolysis is used for removing the starch and protein for preparing the wheat bran meal fiber; and then the fiber passes through a double-screw extruder and enzymolysis is implemented with bacillus subtilis xylanase; the conditionsfor the processes of extrusion and enzymolysis are confirmed; the supernatant liquid after the process of enzymolysis is condensed and dried for preparing the xylo-oligosaccharide. The xylo-oligosaccharide yield with the invention is 11 percent (wheat bran is taken for calculation) and the purity exceeds 70 percent; moreover, content of xylo-disaccharide, xylo- trisaccharide and xylo- tetrasaccharide is more than 50 percent; the wheat bran can be effectively appreciated and utilized and the prepared xylo-oligosaccharide is characterized by high purity, excellent biological activity, excellentbifidobacterium multiplication effect, low heat energy and excellent surface activity.

The present invention discloses xylanase B gene obtained with Thermotoga maritime MSB8 genome DNA and through PCR amplification and the construction of expression vector to express the gene encoded recobinant xylanase in colibacillus. The xylanase has optimal reaction temperature of 90 deg.c, high heat stability in neutral and slight alkali environment, and activity as high as over 1100 times that of cellulase. The xylanase exhibits excellent application foreground as bleaching assistant in pulp producing and papermaking industry. The main product of hydrolyzing xylan is xylobiose, and the present invention is suitable for decomposing xylan in hemicellulose to produce functional oligoxylan.

Xylose-containing plant material may be hydrolyzed to xylose using a β-D-xylosidase which exhibits unexpectedly high activity. The enzyme has a kcat value for catalysis of approximately 185 sec−1 for 1,4-β-D-xylobiose (X2) when measured at a pH of 5.3 and a temperature of 25° C.; this is at least 10-fold greater than reported for other xylosidases at 25° C. and their optimal pH. The enzyme also has an isoelectric point of approximately 4.4. When reacted at a pH between about 4.5 and about 7.7, the β-D-xylosidase exhibits surprisingly high activity for hydrolyzing xylose-containing plant materials to xylose. The xylose released from plant materials may then be converted to other secondary products such as ethanol by fermentation or other reaction. This β-D-xylosidase may be used alone or in combination with other hydrolytic or xylanolytic enzymes for treatment of lignocellulosic or hemicellulosic plant materials or plant material hydrolysates or xylooligosaccharides.

The invention discloses a co-production process for xylobiose and xylose. With the process, high-quality crystallized xylobiose is obtained, and the raw material--xylose for production of xylitol is obtained at the same time. The process comprises the following concrete steps: subjecting a corncob to alkali treatment and then to filtering so as to obtain a dissolved-out solution and separating and purifying xylan by using an ethanol precipitation method; degrading purified xylan with xylanase in an enzymatic membrane reactor to obtain a xylobiose solution and subjecting the xylobiose solution to concentration, decoloring and purifying so as to obtain purified xylobiose liquor; and subjecting the purified xylobiose liquor to purification with a three-component simulated moving bed so as to obtain a xylose component, a xylobiose component and a miscellaneous saccharide component. According to the invention, an enzymatic membrane reactor method is used for enzymatic hydrolysis of xylan, reaction and separation are carried out at the same time, yield of xylobiose is high, cost is low, and the enzymatic membrane reactor method is a key technology of the invention.

The purpose of the present invention is to provide the following: a dried influenza vaccine preparation with which stable retention of the activity of influenza vaccine antigen is possible, even when the preparation is stored without strict low-temperature management, and with which a stable supply is possible; and a method for producing the dried influenza vaccine preparation. The present invention provides a dried influenza vaccine preparation comprising influenza vaccine antigen and a disaccharide, wherein the disaccharide is one or more selected from the group consisting of sucrose, maltose, palatinose, melibiose, isomalt, cellobiose, allolactose, isomaltose, sophorose, lactobionic acid, laminaribiose, xylobiose, turanose, gentiobiose, rutinose, kojibiose, nigerose, robinose, neohesperidose, sucralose, and maltitol.

This invention discloses a method for preparing xylobiose and its specific immobilization enzyme. The method comprises: (1) immobilizing recombined xylanase XynB using metal-chelating epoxy carrier Eupergit C 250 L to obtain immobilization enzyme; (2) suspending the obtained immobilization enzyme in 0.5-1.25 M phosphate buffer with a pH of 5.5-8.0, and reacting at 20-35 deg.C for 12-60 h to obtained immobilization xylanase. The recombinant xylanase XynB is prepared by expressing xylanase gene (Sequence ID 1) in Escherichia coli. The obtained xylanase has such advantages as high recovery rate;high stability and good operation performance after loaded into the column, and can be used for continuous production. The method for preparing xylobiose by using xylanase is simple, and can save xylanase usage, realize separation of enzyme and hydrolyte, largely lower production cost and improve production efficiency, thus is suitable for mass production.

The invention discloses a method for determining xylooligosaccharide through high-efficiency liquid-phase ion exchange chromatography, which comprises the following steps: determining a high-efficiency liquid-phase ion exchange chromatography system and chromatography conditions, determining a standard xylooligosaccharide working formula, determining the chromatography reservation time for xyloheptaose and xylooctaose, and analyzing and determining components of xylooligosaccharide. In the method for determining the xylooligosaccharide through the high-efficiency liquid-phase ion exchange chromatography, a method for accurately and quantitatively determining the xylooligosaccharide through the high-efficiency liquid-phase ion exchange chromatography is established for the first time, the CarboPacTMPA200 (3 * 250m) chromatographic column is adopted, the separation degree and the detection efficiency of xylose and various xylooligosaccharide components are improved significantly through binary gradient elution of sodium acetate and sodium hydroxide, the xylooligosaccharide sample, in particular xylobiose, xylotriose, xyloterose, xylopentaose and xylohexaose, can be accurately and quantitatively determined, and the xyloheptaose and xylooctaose can be analyzed qualitatively. Therefore, the method has great significance in detection, evaluation, promotion and application of xylooligosaccharide products.