61 results about "Xylobiose" patented technology

Isolated and / or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof are provided. Further provided are methods of at least partially degrading xylotriose, xylobiose, and / or arabinofuranose-substituted xylan using isolated and / or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof.

The invention relates to a method for preparing xylooligosaccharides by extruding assisted enzymatic hydrolysis of wheat bran, and specifically belongs to the technical fields of development of agricultural and sideline products, functional food additives and nutrition and health care. It mainly uses wheat bran as raw material, and after crushing, uses enzymatic hydrolysis to remove starch and protein to prepare wheat bran dietary fiber. After passing through a twin-screw extruder, Bacillus subtilis xylanase is used for enzymatic hydrolysis reaction, and the extrusion process is determined. pressure and enzymolysis process conditions; the enzymolysis supernatant is concentrated and dried to prepare xylooligosaccharides. The yield of xylooligosaccharides of the present invention is 11% (calculated by wheat bran), and the purity of xylooligosaccharides exceeds 70%, wherein the content of xylobiose, xylotriose and xylotetraose is more than 50%; Effective value-added and utilization of bran, the prepared xylo-oligosaccharides have high purity, excellent biological activity, good bifidobacteria proliferation effect and low heat performance, and good surface activity.

The invention discloses a preparation method of xylooligosaccharide with low DP (degree of polymerization). The preparation method is characterized by comprising steps as follows: (1) wood fiber biomass is taken as a raw material and subjected to diluted acid / diluted base / hot water pretreatment, a liquid is filtered, a solid phase is treated in a high-temperature degrading or steam explosion pretreatment manner, and the wood fiber biomass is cooled for later use; (2) xylanase is added to a pretreatment solution in step (1) for an enzymatic hydrolysis reaction; (3) an enzymatic hydrolysis solution is treated through enzyme deactivation, purification, concentration and drying, and xylooligosaccharide with low DP is prepared. The DP of finally obtained xylooligosaccharide is low by reasonably optimizing each process step, and xylobiose, xylotriose and xylotetraose are taken as main components, so that the functionality of xylooligosaccharide as a bifidus factor is enhanced. Tests verify that prepared xylooligosaccharide has a good proliferative effect on bifidobacterium animalis and lactobacillus casei. The process is simplified, the product quality is improved, and xylooligosaccharide has good application prospect.

The invention provides a method for extracting xylose, arabinose and galactose from xylose fermentation broth or xylose mother liquor. According to the method, a simulated moving bed chromatographic separation device of sugar alcohol separating resin special for filling is adopted, water serves as an eluent, the separation temperature is 30-90DEG C, the flow rate of feed liquid is controlled at 2-50cm / min, continuous separation and purification of the xylose, the arabinose and the galactose in the xylose fermentation broth or the xylose mother liquor is achieved, and high-purity xylose, arabinose and galactose products are obtained through concentration and crystallization. The method has the advantages of being wide in application range, good in separation effect, high in product purity, environment-friendly and the like.

The invention discloses a high-xylose-tolerance difunctional hemicellulolytic enzyme and a preparation method and application thereof. The amino acid encoding sequence of the enzyme is shown as SEQ ID NO.2 and comprises 542 amino acids, and the theoretical molecular weight of the enzyme is 61.85 kDa. The high-xylose-tolerance difunctional hemicellulolytic enzyme (XylRBM26) has the activity of beta-D-xylosidase and the activity of alpha-L-Arabinfuranosidease, the higher activity (the Ki value is 500 mM) can be kept in a high-xylose environment, the optimum pH is 6.5, and the optimum temperature is 50 DEG C; 95% or above of the activity still can be kept after the enzyme tolerates the condition at 45 DEG C for 60 min, and xylobiose, xylotriose and xylotetraose can be thoroughly hydrolyzed into xylose. The enzyme can serve as a novel enzymic preparation and can be widely applied to food, feed, energy industry and the like.

A process of preparing compound sugar by a-L-Arab carbohydrase. First, prepare solution of wood polyose. Then, add xylanase and a-L-Arab carbohydrase into the solution. Finally, product is obtained after decolour, iron exchange and enrichment from hydrolytic solution.

The invention discloses high-temperature-resistant endoxylanase EpXYN1, coding genes and application thereof. The amino-acid sequence of the high-temperature-resistant endoxylanase EpXYN1 is shown asSEQ ID NO.1. The high-temperature-resistant endoxylanase EpXYN1 is new endoxylanase obtained by cloning in fine eupenicillium, the optimum temperature is 75 DEG C, the optimum pH is 5.5, the high-temperature-resistant endoxylanase EpXYN1 can resist high temperature of 65 DEG C, and has good pH stability, the main hydrolysis product for xylan is xylobiose, and therefore, the high-temperature-resistant endoxylanase EpXYN1 has good application prospect in the industrial application. As novel xylanase, the high-temperature-resistant endoxylanase EpXYN1 can be widely applied in the industries suchas biomass energy, foods, feeds, paper making and medical health care and the like.

The invention relates to a gene XynA of cold adapted endo-beta-xylanase and an application thereof. The genome DNA fragment sequence is shown as SEQ ID N o. 1. The cold adapted beta-xylanase gene XynA is applicable for preparing beta-xylanase XynA. The obtained beta-xylanase XynA is used as neutral cold adapted hydrolase for hydrolyzing oat xylan, beech xylan and birch xylan to produce xylobiose and xylotriose. Optimum enzyme activity temperature is 30 DEG C and optimum pH is 7.0.

By using xylobiose or xylooligosaccharide containing xylobiose as a main ingredient in place of lactulose, there is provided a blood ammonia lowering agent, a therapeutic agent of hyperammonemia or a therapeutic agent of hepatic encephalopathy that need be adminstered in smaller doses and which have no concern over side effects.Lactulose conventionally used as such drugs has to be administered in high doses and involves a safety problem when administered to patients with galactosemia or diabetes mellitus. The drug of the invention which contains xylobiose as a main ingredient solves these problems.

The invention discloses an Aspergillus terreus strain and application thereof in preparing xylnase. The Aspergillus terreus strain FC7-1 is collected under CGMCC No.11657. The invention further provides a method of producing xylnase. The method includes following steps: placing the Aspergillus terreus strain FC7-1 in a fermentation tank for liquid fermentation; collecting a fermentation product, and centrifuging to remove thalli to obtain fermentation supernate which can serve as an xylnase preparation. Optimal pH value of xylnase contained in the xylnase preparation is 5.5, optimal temperature is 55-60 DEG C, and a main product obtained by using the xylnase preparation to hydrolyze bagasse xylan is xylobiose. The Aspergillus terreus strain is of greatly important significance in fully utilizing agricultural waste, increasing comprehensive economic benefit and relieving environmental pressure.

The invention relates to a method for preparing xylo-oligosaccharide which utilizes and extrudes auxiliary enzymolysis wheat bran, in specific belongs to the technology field of agricultural and sideline product development, functional food additive and nutrition healthcare. With the method, wheat bran is taken as the main raw material and the bran is crushed and enzymolysis is used for removing the starch and protein for preparing the wheat bran meal fiber; and then the fiber passes through a double-screw extruder and enzymolysis is implemented with bacillus subtilis xylanase; the conditionsfor the processes of extrusion and enzymolysis are confirmed; the supernatant liquid after the process of enzymolysis is condensed and dried for preparing the xylo-oligosaccharide. The xylo-oligosaccharide yield with the invention is 11 percent (wheat bran is taken for calculation) and the purity exceeds 70 percent; moreover, content of xylo-disaccharide, xylo- trisaccharide and xylo- tetrasaccharide is more than 50 percent; the wheat bran can be effectively appreciated and utilized and the prepared xylo-oligosaccharide is characterized by high purity, excellent biological activity, excellentbifidobacterium multiplication effect, low heat energy and excellent surface activity.

The invention discloses a co-production process for xylobiose and xylose. With the process, high-quality crystallized xylobiose is obtained, and the raw material--xylose for production of xylitol is obtained at the same time. The process comprises the following concrete steps: subjecting a corncob to alkali treatment and then to filtering so as to obtain a dissolved-out solution and separating and purifying xylan by using an ethanol precipitation method; degrading purified xylan with xylanase in an enzymatic membrane reactor to obtain a xylobiose solution and subjecting the xylobiose solution to concentration, decoloring and purifying so as to obtain purified xylobiose liquor; and subjecting the purified xylobiose liquor to purification with a three-component simulated moving bed so as to obtain a xylose component, a xylobiose component and a miscellaneous saccharide component. According to the invention, an enzymatic membrane reactor method is used for enzymatic hydrolysis of xylan, reaction and separation are carried out at the same time, yield of xylobiose is high, cost is low, and the enzymatic membrane reactor method is a key technology of the invention.

The present invention discloses xylanase B gene obtained with Thermotoga maritime MSB8 genome DNA and through PCR amplification and the construction of expression vector to express the gene encoded recobinant xylanase in colibacillus. The xylanase has optimal reaction temperature of 90 deg.c, high heat stability in neutral and slight alkali environment, and activity as high as over 1100 times that of cellulase. The xylanase exhibits excellent application foreground as bleaching assistant in pulp producing and papermaking industry. The main product of hydrolyzing xylan is xylobiose, and the present invention is suitable for decomposing xylan in hemicellulose to produce functional oligoxylan.

Xylose-containing plant material may be hydrolyzed to xylose using a β-D-xylosidase which exhibits unexpectedly high activity. The enzyme has a kcat value for catalysis of approximately 185 sec−1 for 1,4-β-D-xylobiose (X2) when measured at a pH of 5.3 and a temperature of 25° C.; this is at least 10-fold greater than reported for other xylosidases at 25° C. and their optimal pH. The enzyme also has an isoelectric point of approximately 4.4. When reacted at a pH between about 4.5 and about 7.7, the β-D-xylosidase exhibits surprisingly high activity for hydrolyzing xylose-containing plant materials to xylose. The xylose released from plant materials may then be converted to other secondary products such as ethanol by fermentation or other reaction. This β-D-xylosidase may be used alone or in combination with other hydrolytic or xylanolytic enzymes for treatment of lignocellulosic or hemicellulosic plant materials or plant material hydrolysates or xylooligosaccharides.

The invention discloses a co-production process for xylobiose and xylose. With the process, high-quality crystallized xylobiose is obtained, and the raw material--xylose for production of xylitol is obtained at the same time. The process comprises the following concrete steps: subjecting a corncob to alkali treatment and then to filtering so as to obtain a dissolved-out solution and separating and purifying xylan by using an ethanol precipitation method; degrading purified xylan with xylanase in an enzymatic membrane reactor to obtain a xylobiose solution and subjecting the xylobiose solution to concentration, decoloring and purifying so as to obtain purified xylobiose liquor; and subjecting the purified xylobiose liquor to purification with a three-component simulated moving bed so as to obtain a xylose component, a xylobiose component and a miscellaneous saccharide component. According to the invention, an enzymatic membrane reactor method is used for enzymatic hydrolysis of xylan, reaction and separation are carried out at the same time, yield of xylobiose is high, cost is low, and the enzymatic membrane reactor method is a key technology of the invention.

The purpose of the present invention is to provide the following: a dried influenza vaccine preparation with which stable retention of the activity of influenza vaccine antigen is possible, even when the preparation is stored without strict low-temperature management, and with which a stable supply is possible; and a method for producing the dried influenza vaccine preparation. The present invention provides a dried influenza vaccine preparation comprising influenza vaccine antigen and a disaccharide, wherein the disaccharide is one or more selected from the group consisting of sucrose, maltose, palatinose, melibiose, isomalt, cellobiose, allolactose, isomaltose, sophorose, lactobionic acid, laminaribiose, xylobiose, turanose, gentiobiose, rutinose, kojibiose, nigerose, robinose, neohesperidose, sucralose, and maltitol.

This invention discloses a method for preparing xylobiose and its specific immobilization enzyme. The method comprises: (1) immobilizing recombined xylanase XynB using metal-chelating epoxy carrier Eupergit C 250 L to obtain immobilization enzyme; (2) suspending the obtained immobilization enzyme in 0.5-1.25 M phosphate buffer with a pH of 5.5-8.0, and reacting at 20-35 deg.C for 12-60 h to obtained immobilization xylanase. The recombinant xylanase XynB is prepared by expressing xylanase gene (Sequence ID 1) in Escherichia coli. The obtained xylanase has such advantages as high recovery rate;high stability and good operation performance after loaded into the column, and can be used for continuous production. The method for preparing xylobiose by using xylanase is simple, and can save xylanase usage, realize separation of enzyme and hydrolyte, largely lower production cost and improve production efficiency, thus is suitable for mass production.

The invention discloses a multipurpose wastewater treatment agent. The multipurpose wastewater treatment agent is prepared from the components in parts by weight: 2-8 parts of kaolin, 2-7 parts of aluminum oxide, 4-11 parts of kieselguhr, 3-9 parts of tea saponin, 2-8 parts of alum, 1-10 parts of activated carbon, 2-11 parts of bamboo charcoal powder, 2-13 parts of sepiolite, 3-15 parts of tannin,and 2-12 parts of xylobiose. The multipurpose wastewater treatment agent is easy to use, all components only need to be mixed according to the proportion to be directly placed in wastewater, pollution to the environment is avoided, influence of environment factors is avoided, large pollutants in the wastewater can be adsorbed and precipitated, the pungent smell in the wastewater is adsorbed, thecatalytic degradation effect can be achieved on harmful substances, damage to wastewater treatment equipment is avoided, the using amount is small, the input cost is low, the effect is obvious and efficient, the working strength of workers is lowered, the working efficiency of the workers is improved, and the difficulties that an existing wastewater treatment agent is poor in effect, treating is difficult, the cost is high, secondary pollution is caused, and the existing wastewater treatment agent is influenced by the environment are overcome.

The invention discloses a method for determining xylooligosaccharide through high-efficiency liquid-phase ion exchange chromatography, which comprises the following steps: determining a high-efficiency liquid-phase ion exchange chromatography system and chromatography conditions, determining a standard xylooligosaccharide working formula, determining the chromatography reservation time for xyloheptaose and xylooctaose, and analyzing and determining components of xylooligosaccharide. In the method for determining the xylooligosaccharide through the high-efficiency liquid-phase ion exchange chromatography, a method for accurately and quantitatively determining the xylooligosaccharide through the high-efficiency liquid-phase ion exchange chromatography is established for the first time, the CarboPacTMPA200 (3 * 250m) chromatographic column is adopted, the separation degree and the detection efficiency of xylose and various xylooligosaccharide components are improved significantly through binary gradient elution of sodium acetate and sodium hydroxide, the xylooligosaccharide sample, in particular xylobiose, xylotriose, xyloterose, xylopentaose and xylohexaose, can be accurately and quantitatively determined, and the xyloheptaose and xylooctaose can be analyzed qualitatively. Therefore, the method has great significance in detection, evaluation, promotion and application of xylooligosaccharide products.