61 results about "Xylobiose" patented technology

Isolated and / or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof are provided. Further provided are methods of at least partially degrading xylotriose, xylobiose, and / or arabinofuranose-substituted xylan using isolated and / or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof.

The invention relates to a method for preparing xylo-oligosaccharides by utilizing extrusion-assisted enzymatic hydrolysis of wheat bran, and specifically belongs to the technical fields of agricultural and sideline product development, functional food additives and nutritional health care. It mainly uses wheat bran as raw material. After crushing, enzymatic decomposition is used to remove starch and protein to prepare wheat bran dietary fiber. After passing through a twin-screw extruder, Bacillus subtilis xylanase is used for enzymatic hydrolysis reaction to determine the extrusion rate. Pressure and enzymatic hydrolysis process conditions; the enzymatic hydrolysis supernatant is concentrated and dried to prepare xylo-oligosaccharides. The yield rate of xylo-oligosaccharides of the present invention is 11% (calculated based on wheat bran), the purity of xylo-oligosaccharides exceeds 70%, and the content of xylobiose, xylotriose and xylotetraose is more than 50%; wheat can be realized Effective value addition and utilization of bran, the prepared xylo-oligosaccharides have high purity, excellent biological activity, good bifidobacteria proliferation effect, low heat performance and good surface activity.

The invention discloses a preparation method of xylooligosaccharide with low DP (degree of polymerization). The preparation method is characterized by comprising steps as follows: (1) wood fiber biomass is taken as a raw material and subjected to diluted acid / diluted base / hot water pretreatment, a liquid is filtered, a solid phase is treated in a high-temperature degrading or steam explosion pretreatment manner, and the wood fiber biomass is cooled for later use; (2) xylanase is added to a pretreatment solution in step (1) for an enzymatic hydrolysis reaction; (3) an enzymatic hydrolysis solution is treated through enzyme deactivation, purification, concentration and drying, and xylooligosaccharide with low DP is prepared. The DP of finally obtained xylooligosaccharide is low by reasonably optimizing each process step, and xylobiose, xylotriose and xylotetraose are taken as main components, so that the functionality of xylooligosaccharide as a bifidus factor is enhanced. Tests verify that prepared xylooligosaccharide has a good proliferative effect on bifidobacterium animalis and lactobacillus casei. The process is simplified, the product quality is improved, and xylooligosaccharide has good application prospect.

The invention provides a method for extracting xylose, arabinose and galactose from xylose fermentation broth or xylose mother liquor. According to the method, a simulated moving bed chromatographic separation device of sugar alcohol separating resin special for filling is adopted, water serves as an eluent, the separation temperature is 30-90DEG C, the flow rate of feed liquid is controlled at 2-50cm / min, continuous separation and purification of the xylose, the arabinose and the galactose in the xylose fermentation broth or the xylose mother liquor is achieved, and high-purity xylose, arabinose and galactose products are obtained through concentration and crystallization. The method has the advantages of being wide in application range, good in separation effect, high in product purity, environment-friendly and the like.

The present invention discloses xylanase B gene obtained with Thermotoga maritime MSB8 genome DNA and through PCR amplification and the construction of expression vector to express the gene encoded recobinant xylanase in colibacillus. The xylanase has optimal reaction temperature of 90 deg.c, high heat stability in neutral and slight alkali environment, and activity as high as over 1100 times that of cellulase. The xylanase exhibits excellent application foreground as bleaching assistant in pulp producing and papermaking industry. The main product of hydrolyzing xylan is xylobiose, and the present invention is suitable for decomposing xylan in hemicellulose to produce functional oligoxylan.

The invention relates to a method of extracting xylo-oligosaccharide from bamboo shoot shells and separating to obtain a xylo-oligosaccharide monomer. The method comprises the following steps: (a) after performing alkaline degradation on the bamboo shoot shells, performing microwave digestion, and adding xylanase to perform enzymolysis to obtain a liquid supernatant; (b) balancing the volume of three active carbon columns by using 50-70% ethanol at a flowing speed of 2-3ml / min, then balancing the volume of the three active carbon columns by using deionized water at a speed of 2-3ml / min, wherein the diameters of the active carbon columns are 3.5 centimeters, the heights of the active carbon columns are 45 centimeters, and active carbon used for chromatography, which is added into the active carbon column is 85g; (c) adding the xylo-oligosaccharide liquid supernatant, eluting 1-3 columns by using the deionized water at a flowing speed of 2-3ml / min, linearly eluting 7-10 columns by using 0.1-37% ethanol at the same flowing speed of 2-3ml / min, and collecting an ethanol eluting part. The method is high in xylo-oligosaccharide extraction rate, easy to operate and good in repeatability; a high-purity monomer can be obtained by separating a xylo-oligosaccharide water solution with low contents of xylobiose to xylopentaose.

The invention discloses a high-xylose-tolerance difunctional hemicellulolytic enzyme and a preparation method and application thereof. The amino acid encoding sequence of the enzyme is shown as SEQ ID NO.2 and comprises 542 amino acids, and the theoretical molecular weight of the enzyme is 61.85 kDa. The high-xylose-tolerance difunctional hemicellulolytic enzyme (XylRBM26) has the activity of beta-D-xylosidase and the activity of alpha-L-Arabinfuranosidease, the higher activity (the Ki value is 500 mM) can be kept in a high-xylose environment, the optimum pH is 6.5, and the optimum temperature is 50 DEG C; 95% or above of the activity still can be kept after the enzyme tolerates the condition at 45 DEG C for 60 min, and xylobiose, xylotriose and xylotetraose can be thoroughly hydrolyzed into xylose. The enzyme can serve as a novel enzymic preparation and can be widely applied to food, feed, energy industry and the like.

A process of preparing compound sugar by a-L-Arab carbohydrase. First, prepare solution of wood polyose. Then, add xylanase and a-L-Arab carbohydrase into the solution. Finally, product is obtained after decolour, iron exchange and enrichment from hydrolytic solution.

The invention discloses high-temperature-resistant endoxylanase EpXYN1, coding genes and application thereof. The amino-acid sequence of the high-temperature-resistant endoxylanase EpXYN1 is shown asSEQ ID NO.1. The high-temperature-resistant endoxylanase EpXYN1 is new endoxylanase obtained by cloning in fine eupenicillium, the optimum temperature is 75 DEG C, the optimum pH is 5.5, the high-temperature-resistant endoxylanase EpXYN1 can resist high temperature of 65 DEG C, and has good pH stability, the main hydrolysis product for xylan is xylobiose, and therefore, the high-temperature-resistant endoxylanase EpXYN1 has good application prospect in the industrial application. As novel xylanase, the high-temperature-resistant endoxylanase EpXYN1 can be widely applied in the industries suchas biomass energy, foods, feeds, paper making and medical health care and the like.

The invention discloses a method for detecting gas chromatogram of xylobiose and relates to xylobiose. The method comprises the following steps: adding NaBH4 and deionized water to a xylobiose-containing xylooligosaccharide sample and ribitol interior label, performing reaction, adding acetic acid until air bubbles are not generated, adding methanol, performing vacuum concentration until the mixture is dried, adding acetic anhydride and pyridine, continuing to react, adding ethyl acetate for extracting a derived product, and washing to obtain a standard sample to be used for gas chromatography; setting operating conditions of the instrument, wherein the temperature of a gas chromatographic column is 150-400 DEG C, the flow velocity of a mobile phase is 0.1-10.0mL.min<-1>, the temperature of a detector is 150-400 DEG C; performing sample introduction on a solution to be detected into an injection hole, detecting and recording a chromatogram, and analyzing the components by using a gas chromatograph-mass spectrometer; and acquiring a xylobiose calibration curve by means of analysis software, performing quantitative analysis on xylobiose in the sample, and judging the content of the xylobiose according to a ratio of peak elution area of a xylobiose peak to area of an internal standard peak.

The invention discloses a method for crystallizing xylobiose, namely 1 to 80g of inorganic salt and 0.67 to 19L of organic solvent are added into each liter of xylobiose solution with a pH value of between 4.0 and 9.0 and a concentration of between 10 and 200g / L, the crystallization temperature is controlled to be between 10 and 40 DEG C, the stirring rotating speed is controlled to be between 10 and 500r / min, the filtration is performed after the crystallization is finished, the same organic solvent is used to wash, and xylobiose crystals can be obtained after vacuum drying. The method has steady crystallization yield, improves the quality of products, improves the appearance and the particle homogeneity of the products, has simple and convenient operation and good repetitiveness, and is suitable for mass production.

The invention relates to a gene XynA of cold adapted endo-beta-xylanase and an application thereof. The genome DNA fragment sequence is shown as SEQ ID N o. 1. The cold adapted beta-xylanase gene XynA is applicable for preparing beta-xylanase XynA. The obtained beta-xylanase XynA is used as neutral cold adapted hydrolase for hydrolyzing oat xylan, beech xylan and birch xylan to produce xylobiose and xylotriose. Optimum enzyme activity temperature is 30 DEG C and optimum pH is 7.0.

The present invention provides a dried influenza vaccine preparation in which the activity of an influenza vaccine antigen can be stably maintained even when stored without strictly maintaining a low temperature, and which can be stably supplied. The present invention also provides a method of producing the dried influenza vaccine preparation. The present invention provides a dried influenza vaccine preparation containing an influenza vaccine antigen and a disaccharide, wherein the disaccharide is at least one selected from the group consisting of sucrose, maltose, palatinose, melibiose, isomalt, cellobiose, allolactose, isomaltose, sophorose, lactobionic acid, laminaribiose, xylobiose, turanose, gentiobiose, rutinose, kojibiose, nigerose, robinose, neohesperidose, sucralose, and maltitol.

The invention discloses an aspergillus sp. strain and application of the aspergillus sp. strain to the preparation of xylanase. The aspergillus sp. strain FC2-2 provided by the invention has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6049. The invention also provides a method for producing the xylanase. The method comprises the following steps that the aspergillus sp. strain FC2-2 is subjected to liquid fermentation, fermentation products are collected, centrifugation is carried out for removing thalli, then, obtained fermentation supernatant can be used as a xylanase preparation, the most proper pH value of xylanase contained in the xylanase preparation is 5.5, the most proper temperature is 60 DEG C, the xylanase has better stability under the condition of 50 DEG C, and in addition, major products of hydrolysis bagasse xylanase are xylobiose. The xylanase preparation has application potential in the production of health care products of xylobiose.

By using xylobiose or xylooligosaccharide containing xylobiose as a main ingredient in place of lactulose, there is provided a blood ammonia lowering agent, a therapeutic agent of hyperammonemia or a therapeutic agent of hepatic encephalopathy that need be adminstered in smaller doses and which have no concern over side effects.Lactulose conventionally used as such drugs has to be administered in high doses and involves a safety problem when administered to patients with galactosemia or diabetes mellitus. The drug of the invention which contains xylobiose as a main ingredient solves these problems.

The invention discloses an Aspergillus terreus strain and application thereof in preparing xylnase. The Aspergillus terreus strain FC7-1 is collected under CGMCC No.11657. The invention further provides a method of producing xylnase. The method includes following steps: placing the Aspergillus terreus strain FC7-1 in a fermentation tank for liquid fermentation; collecting a fermentation product, and centrifuging to remove thalli to obtain fermentation supernate which can serve as an xylnase preparation. Optimal pH value of xylnase contained in the xylnase preparation is 5.5, optimal temperature is 55-60 DEG C, and a main product obtained by using the xylnase preparation to hydrolyze bagasse xylan is xylobiose. The Aspergillus terreus strain is of greatly important significance in fully utilizing agricultural waste, increasing comprehensive economic benefit and relieving environmental pressure.

The invention relates to a method for preparing xylo-oligosaccharide which utilizes and extrudes auxiliary enzymolysis wheat bran, in specific belongs to the technology field of agricultural and sideline product development, functional food additive and nutrition healthcare. With the method, wheat bran is taken as the main raw material and the bran is crushed and enzymolysis is used for removing the starch and protein for preparing the wheat bran meal fiber; and then the fiber passes through a double-screw extruder and enzymolysis is implemented with bacillus subtilis xylanase; the conditionsfor the processes of extrusion and enzymolysis are confirmed; the supernatant liquid after the process of enzymolysis is condensed and dried for preparing the xylo-oligosaccharide. The xylo-oligosaccharide yield with the invention is 11 percent (wheat bran is taken for calculation) and the purity exceeds 70 percent; moreover, content of xylo-disaccharide, xylo- trisaccharide and xylo- tetrasaccharide is more than 50 percent; the wheat bran can be effectively appreciated and utilized and the prepared xylo-oligosaccharide is characterized by high purity, excellent biological activity, excellentbifidobacterium multiplication effect, low heat energy and excellent surface activity.

The invention discloses a co-production process for xylobiose and xylose. With the process, high-quality crystallized xylobiose is obtained, and the raw material--xylose for production of xylitol is obtained at the same time. The process comprises the following concrete steps: subjecting a corncob to alkali treatment and then to filtering so as to obtain a dissolved-out solution and separating and purifying xylan by using an ethanol precipitation method; degrading purified xylan with xylanase in an enzymatic membrane reactor to obtain a xylobiose solution and subjecting the xylobiose solution to concentration, decoloring and purifying so as to obtain purified xylobiose liquor; and subjecting the purified xylobiose liquor to purification with a three-component simulated moving bed so as to obtain a xylose component, a xylobiose component and a miscellaneous saccharide component. According to the invention, an enzymatic membrane reactor method is used for enzymatic hydrolysis of xylan, reaction and separation are carried out at the same time, yield of xylobiose is high, cost is low, and the enzymatic membrane reactor method is a key technology of the invention.

Xylose-containing plant material may be hydrolyzed to xylose using a β-D-xylosidase which exhibits unexpectedly high activity. The enzyme has a kcat value for catalysis of approximately 185 sec−1 for 1,4-β-D-xylobiose (X2) when measured at a pH of 5.3 and a temperature of 25° C.; this is at least 10-fold greater than reported for other xylosidases at 25° C. and their optimal pH. The enzyme also has an isoelectric point of approximately 4.4. When reacted at a pH between about 4.5 and about 7.7, the β-D-xylosidase exhibits surprisingly high activity for hydrolyzing xylose-containing plant materials to xylose. The xylose released from plant materials may then be converted to other secondary products such as ethanol by fermentation or other reaction. This β-D-xylosidase may be used alone or in combination with other hydrolytic or xylanolytic enzymes for treatment of lignocellulosic or hemicellulosic plant materials or plant material hydrolysates or xylooligosaccharides.

The invention discloses a multipurpose wastewater treatment agent. The multipurpose wastewater treatment agent is prepared from the components in parts by weight: 2-8 parts of kaolin, 2-7 parts of aluminum oxide, 4-11 parts of kieselguhr, 3-9 parts of tea saponin, 2-8 parts of alum, 1-10 parts of activated carbon, 2-11 parts of bamboo charcoal powder, 2-13 parts of sepiolite, 3-15 parts of tannin,and 2-12 parts of xylobiose. The multipurpose wastewater treatment agent is easy to use, all components only need to be mixed according to the proportion to be directly placed in wastewater, pollution to the environment is avoided, influence of environment factors is avoided, large pollutants in the wastewater can be adsorbed and precipitated, the pungent smell in the wastewater is adsorbed, thecatalytic degradation effect can be achieved on harmful substances, damage to wastewater treatment equipment is avoided, the using amount is small, the input cost is low, the effect is obvious and efficient, the working strength of workers is lowered, the working efficiency of the workers is improved, and the difficulties that an existing wastewater treatment agent is poor in effect, treating is difficult, the cost is high, secondary pollution is caused, and the existing wastewater treatment agent is influenced by the environment are overcome.

The invention provides a preparation method of hybrid xylanase atxb. The method enables connecting sequence of brown high temperature single spore bacterium xylanase A and a xylan binding domain to be fused at the tail end C of hybrid xylanase atx to obtain the hybrid xylanase atxb. Birchwood xylan is hydrolyzed by hybrid enzyme to obtain xylo-oligosaccharide mainly, main products are xylbiose and xylotriose, the xylbiose and the xylotriose are main components of functional xylo-oligosaccharide, and prebiotic effect of the functional xylo-oligosaccharide is remarkably higher than other xylo-oligosaccharide. In addition, a hybrid gene expression adopts a pichia pastoris expression system, and the system has the advantages of being safe, free of toxic metabolite secretion and easy to culture. Compared with an escherichia coli expression system and an animal cell expression system, the pichia pastoris expression system has remarkable advantages so as to enable fermentation production of the hybrid xylanase atxb to have remarkable advantages.

The invention relates to the biochemical field, in particular to a protein RSipoEnXyn10A and its application as an endoxylanase in the production of xylose. The protein RSipoEnXyn10A is a protein represented by the following 1) or 2): 1) a protein composed of an amino acid sequence represented by SEQ ID NO: 3 in the sequence list; 2) a protein derive from 1) which is formed by substitute, deletingor adding one or more amino acid residues to that amino acid sequence shown in SEQ ID NO: 3 in the sequence table and has the same function. The invention obtains a gene SipoEnXyn10A encoding an endoxylanase from the genome sequence of Streptomyces suavissimus, the gene can be expressed in host cells to produce the protein RSipoEnXyn10A. The protein RSipoEnXyn10A is used as an endoxylanase in theproduction of xylenedisaccharide. The endoxylanase is a kind of endoxylanase with the properties of high temperature, neutrality and xylenedisaccharide release from xylan hydrolyzed from agroforestryresidues, which is of great significance to the production of xylenedisaccharide.

The invention discloses a method for determining xylooligosaccharide through high-efficiency liquid-phase ion exchange chromatography, which comprises the following steps: determining a high-efficiency liquid-phase ion exchange chromatography system and chromatography conditions, determining a standard xylooligosaccharide working formula, determining the chromatography reservation time for xyloheptaose and xylooctaose, and analyzing and determining components of xylooligosaccharide. In the method for determining the xylooligosaccharide through the high-efficiency liquid-phase ion exchange chromatography, a method for accurately and quantitatively determining the xylooligosaccharide through the high-efficiency liquid-phase ion exchange chromatography is established for the first time, the CarboPacTMPA200 (3 * 250m) chromatographic column is adopted, the separation degree and the detection efficiency of xylose and various xylooligosaccharide components are improved significantly through binary gradient elution of sodium acetate and sodium hydroxide, the xylooligosaccharide sample, in particular xylobiose, xylotriose, xyloterose, xylopentaose and xylohexaose, can be accurately and quantitatively determined, and the xyloheptaose and xylooctaose can be analyzed qualitatively. Therefore, the method has great significance in detection, evaluation, promotion and application of xylooligosaccharide products.

The invention relates to a method for preparing xylooligosaccharide from lignocellulose. The method comprises the following steps: performing superfine pulverization on the raw material lignocelluloses, performing hydrothermal pretreatment, performing enzymolysis reaction and performing enzyme deactivation. The xylobiose content of xylooligosaccharide extracting liquid is high, the extraction rateof the xylooligosaccharide is more excellent than that of an alkali treatment scheme, pollution is avoided, the enzymolysis reaction efficiency is high, and a new technical scheme is provided for industrialized application of the lignocellulose such as corncobs.

The invention discloses an application of a trichoderma pleuroides strain ZJ-03 in deep processing of industrial cannabis sativa, and relates to the technical field of food processing. The invention aims to solve the problems of how to induce trichoderma pleuroides to produce xylanase with high enzyme activity and how to prepare a high-purity healthy syrup product with xylobiose and xylotriose asmain components. The method comprises the following steps: adding China hemp scrap powder and China hemp seed meal powder into a nutrient solution, stirring, and inoculating trichoderma pleuroticum ZJ-03 spore suspension; inoculating, putting into a fermentation chamber, culturing to obtain a trichoderma pleuroides solid fermentation material, performing standing and leaching to obtain a leached solid-liquid mixture, roughly filtering, centrifuging, and taking a supernatant to obtain a crude sugar solution; sequentially carrying out primary evaporation and concentration, primary ion exchange,secondary evaporation and concentration, secondary ion exchange and tertiary evaporation and concentration on the crude sugar solution to obtain a functional syrup product. The trichoderma pleuroticumstrain ZJ-03 can be obtained and applied to deep processing of industrial cannabis sativa.