Preparation of oligose from alpha-L-arabglycosidase

A technology of arabinosidase and xylooligosaccharides, which is applied in the field of preparation of xylooligosaccharides by α-L-arabinosidase, which can solve the problems of affecting the efficiency of enzymatic hydrolysis, inability to combine and decompose, and affecting the quality of xylooligosaccharides , to achieve the effect of improving quality and purity, increasing content, and increasing xylobiose content

Inactive Publication Date: 2005-10-12
NANJING NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, about 94% of the hemicellulose in agricultural and forestry wastes such as corncobs, bagasse and straw is arabinose glucuronoxylan, which is a chain linked by β-1,4 glycosidic bonds. The main chain of xylan has side branches composed of 1,3-arabinose or 1,2-glucuronic acid. When a single xylanase is used to hydrolyze this type of xylan to prepare xylo-oligosaccharides, these side branches are Create a space barrier to the action of xylanase, so that xylanase cannot bind and decompose its adjacent xylan, which not only affects the efficiency of enzymatic hydrolysis, but also affects the quality of xylooligosaccharides

Method used

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  • Preparation of oligose from alpha-L-arabglycosidase
  • Preparation of oligose from alpha-L-arabglycosidase
  • Preparation of oligose from alpha-L-arabglycosidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: (1) get the air-dried corn cob fiber that particle diameter is about 3mm, centrifugally remove moisture after soaking with clear water earlier, add sodium hydroxide solution by solid-liquid ratio 7~10: 1, final concentration is 1%~3 %, leaching at room temperature or high temperature, then collecting the filtrate and neutralizing it to slightly acidic with acid, and then precipitating with three times the volume of ethanol to obtain xylan.

[0027] (2) Preparation of heat-resistant enzyme

[0028]Two pairs of primers were designed according to the sequences of xynB and ara in Genbank, and the genomic DNA of T. maritima was used as a template for PCR amplification. The Tm-xymB-N-terminal primer is (5'-CCGTTCCATGGACTACAGGATGTGC-3'), and the Tm-xymB-C-terminal primer is (5'-CCGCTCGAGCGGATATATCTTTTCTTCCCTT-3'). Tm-ara-N: 5-GGGGGTACCATGTCCTACAGGATAGTG-3 with EcoRV restriction site and start codon; Tm-ara-C: 5-AACTGCAGTCACTCGAGCAATTCTACCTCAAT-3 with an XhoI res...

Embodiment 2

[0036] Example 2: basically the same as Example 1, the difference is that the hydrolysis temperature is 95°C, the xylan is hydrolyzed for 12 hours, and samples are taken for TLC analysis at 1.5h, 3h, 5h, 8h, and 12h of hydrolysis respectively, as figure 2 Shown; Hydrolysis 12 hours gained product carries out ion chromatographic analysis as Figure 4 shown.

Embodiment 3

[0037] Example 3: Basically the same as Example 1, the difference is that only heat-resistant xylanase is added to carry out xylan hydrolysis at 95°C for 12 hours, and samples are taken at 1.5h, 3h, 5h, 8h, and 12h of hydrolysis Perform TLC analysis, such as figure 2 Shown; Hydrolysis 12 hours gained product carries out ion chromatographic analysis as image 3 shown.

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Abstract

A process of preparing compound sugar by a-L-Arab carbohydrase. First, prepare solution of wood polyose. Then, add xylanase and a-L-Arab carbohydrase into the solution. Finally, product is obtained after decolour, iron exchange and enrichment from hydrolytic solution.

Description

technical field [0001] The invention relates to a method for preparing xylooligosaccharides, in particular to a method for preparing xylooligosaccharides by using α-L-arabinosidase. Background technique [0002] Bifidobacteria are the most important beneficial bacteria in the human intestinal tract. They mainly exist in the large intestine of the human body. They have various health effects on the human body, improving the intestinal environment, inhibiting pathogenic bacteria, regulating immunity, synthesizing B vitamins, and improving calcium absorption. , reduce blood ammonia level and cholesterol concentration, inhibit the occurrence of cancer, treat digestive tract dysfunction and anti-tumor effects. Various studies have shown that xylooligosaccharides, as a bifidus factor, are superior to other active oligosaccharides, especially xylobiose, which has significant bifidobacteria proliferation ability, non-digestibility, no dental caries, and promotes the body's response ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/08
Inventor 邵蔚蓝薛业敏
Owner NANJING NORMAL UNIVERSITY
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