61 results about "Blood ammonia" patented technology

The invention relates to a method for determining the content of blood ammonia, and also the reagent kit for blood ammonia diagnosis. The reagent kit comprises cushioning solution, adenosine triphosphate, phosphoenolpyruvate phosphatase, deacidized type coenzyme, ammonia kinase, pyruvate kinase, lactate dehydrogenase, and stabilizer. By mixing sample and reagent of a predetermiend volumetric ratio, generating coupling reaction between them, subjecting the final reactant to biochemiscal analyser, the main wavelength absorbancy variance ratio (speed) can be detected, and the blood ammonia content can thus be measured. The method of the invention can be used to obtain the needed measurement result purely through biochemical analytic instruments, and advantages of the method include higher sensibility, better accuracy, less susceptibility to contamination of internal or external materials, and easy application.

The invention is about a method of measuring the content of blood ammonia, and it also concerns the reagent box of blood ammonia diagnosis. This invention belongs to the field of medical testing and measuring technology. The reagent box is consisted of buffer solution, 2ú¡ketoglutarate, reduced coenzyme, adenosine triphosphate, phosphoenolpyruvate, glutamate dehydrogenase, glutamate kinase, pyruvate kinase, lactate dehydrogenase and stabilizer. Firstly, we cause an enzyme-coupled reaction through mixing the sample and the reagent according to a certain proportion of volume; secondly, put the final reactant under the biochemical analyzer and test the absorbance variational situation (speed) of dominant wavelength; then we can get the content of blood ammonia. By using this invention, we can get the necessary measuring result with high sensitiveness and fine precision through biochemical analyzer, and the result would not be contaminated by material of internal and exogenous sources. Thus, this method can be conveniently promoted and applied.

The invention discloses anti-aging probiotics, and belongs to the field of all related food industries of probiotics. The anti-aging probiotics comprise lactobacillus paracasei combined melon powder.The effective amount of the lactobacillus paracasei combined melon powder is 10-60 billion per day for supplement, namely 10<10> cfu/day-6*10<10> cfu/day of lactobacillus paracasei and 1.2-2.6 g of melon powder per day. According to the method for using the lactobacillus paracasei combined melon powder as a food supplement or medicine for resisting oxidation stress and delaying aging, the lactobacillus paracasei combined melon powder is used to prepare a composition for improving body composition, improving body function or reducing fatigue after exercise. When being used as a food supplementor medicine for improving oxidation stress in the environment and for sports training, the composition has the advantages of not containing animal allergen, and can achieve the effects of improving expression of antioxidant and anti-inflammatory factors, delaying aging, relaxing mood, increasing muscle endurance, reducing lactic acid concentration and blood ammonia concentration in blood after sports, and the like.

The invention provides a slow-release preparation of ornithine aspartate, the slow-release preparation comprises major components of the ornithine aspartate and an auxiliary additive, and controlled release of active ingredients in the preparation can be realized by the auxiliary additive. The preparation prepared into a capsule preparation formulation overcomes the defects that other ornithine aspartate preparations such as an injection are high in concentration, inconvenient to store and transport, and difficult to use for a patient by oneself, and the preparation has the advantages of convenience for middle and old age patients to take, quick absorption, high biological utilization rate and the like. The slow-release preparation of the ornithine aspartate is suitable for blood ammonia rising caused by acute and chronic liver diseases (such as various types of hepatitis, liver cirrhosis, fatty liver and post-hepatitis syndrome) and treatment of hepatic encephalopathy such as potential or attack-stage hepatic encephalopathy accompanying with or secondary with liver detoxification function damage (such as liver cirrhosis), and is especially suitable for the treatment of consciousness clouding state and other diseases in an early hepatic coma period and a hepatic coma period.

The present invention relates to a compound of Antrodia camphorata used to delay physiological fatigue, in particular to a extract isolated from Antrodia camphorata4-hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone, and its use in delaying fatigue. Immediate supplementation of the compound according to the invention after high intensity exhausted exercise at 80%VO₂max can promote the metabolism of creatine phosphate kinase and blood ammonia in human bodies, be beneficial to their recovery, further achieves the function in delaying physiological fatigue.

A diagnostic kit of blood ammonia consists of buffer solution, 2 ¿C ketoglutarate, reduced coenzyme, glutamate dehydrogenase and stabilizer. Its measuring method includes mixing sample with reagent in certain volume ratio to let them generate enzyme couple reaction, placing reacted matter under biochemical analyzer for detecting absorbance variation of main wavelength so as to measure and calculating out content of blood ammonia.

The invention discloses a method for measuring ammonia content in serum and namely discloses a dual reagent for measuring ammonia content in serum. Accelerated destruction tests of the reagent at 37 DEG C and 42 DEG C show that the reagent is good in stability, so that the complex problem that a freeze-drying agent needs freeze-drying and re-dissolving is solved; the reagent can be prepared when being used; the reagent is capable of stabilizing for 20 months at 2-8 DEG C. Clinical correlation tests show that the method is stable in measurement value, high in accuracy, applicable to manual, semi-automatic and automatic detection systems, and easy to popularize and apply.

The invention is about a method of measuring the content of blood ammonia, and it also concerns the reagent box of blood ammonia diagnosis. This invention belongs to the field of medical testing and measuring technology. The reagent box is consisted of buffer solution, glutamate, 2ú¡ketoglutarate, reduced coenzyme, adenosine triphosphate, glutamine synthetase, glutamate synthetase and stabilizer. Firstly, we cause an enzyme-coupled reaction through mixing the sample and the reagent according to a certain proportion of volume; secondly, put the final reactant under the biochemical analyzer and test the absorbance variational situation (speed) of dominant wavelength; then we can get the content of blood ammonia. By using this invention, we can get the necessary measuring result with high sensitiveness and fine precision through biochemical analyzer, and the result would not be contaminated by material of internal and exogenous sources. Thus, this method can be conveniently promoted and applied.

The invention discloses a liquid monomer reagent for determining the content of ammonia in blood serum, a preparation method and an application thereof. The liquid monomer reagent comprises the following components: buffering solution, glutamate dehydrogenase, alpha-oxoglutarate, reduction type nicotinamide adenine dinucleotide, glucose, glucose dehydrogenase and a stabilizing agent, wherein the enzyme activity ratio of the glutamate dehydrogenase and the glucose dehydrogenase is (3-3000):1. Shown by accelerating destruction test at the temperature of 37 DEG C and 42 DEG C, the blood-ammonia liquid monomer reagent is good in stability, solves the fussy problem that a lyophilized agent needs to be lyophilized and redissolved, can be prepared and used at any time, and can be stable for 18 mouths at the temperature of 2-8 DEG C; and shown by clinic correlation test, the liquid monomer reagent is stable in blood-ammonia determination, is high in determined-value accuracy, is applicable to manual, semi-automatic and automatic detection system and is easily promoted and used in all hospitals.

The invention belongs to the technical field of chicken breeding, and particularly discloses a local-cock breeding method. The local-cock breeding method includes the following specific steps of (1) site selection and construction of chicken farms; (2) daily feeding of feed; (3) regular nutritional supplement. According to the local-cock breeding method, the disease resisting immunity of cocks is enhanced, movement is agile, vision is good, skeletons are robust, feathers are full, the chicken is thin in skin, tender in meat, small in fat amount and bright in color and does not taste greasy during long-time chewing, eaters are effectively prevented from getting fat after eating, ammonia metabolism is effectively promoted, the blood ammonia concentration is reduced, the liver is protected, the disease-resistant, antibacterial and anti-stress capacities of the cocks are improved, gastrointestinal tract wriggling is promoted, growth and reproduction of probiotics of gastrointestinal tracts are promoted, various nutrient substances can be smoothly decomposed, the nutrition conversion efficiency is improved, growth of the cocks is quickened accordingly, and the nutrient content of the meat quality is increased.

The invention provides an acylated derivative for an ornithine and aspartate dipeptide compound or an officinal salt thereof, a preparation method thereof, a medicine composition containing the derivative or the officinal salt thereof, and the application of the derivative or the officinal salt thereof for preparing medicines for preventing or curing hyperammonemia or liver diseases, particularlyhepatic encephalopathy. Experimental results definitely indicate that the concentration of blood ammonia can be obviously reduced after administration for the acylated derivative for the ornithine andaspartate dipeptide compound, the acylated derivative takes effect quickly by preferably compared with LOLA, subsequent dysmnesia after TAA-induced rat liver injury can be obviously improved, and theacylated derivative for the dipeptide has a certain treatment effect on hyperammonemia or liver diseases, particularly hepatic encephalopathy.

The invention relates to an Antrodia cinnamomea compound which can slow down physiological fatigue, in particular to a 4-hydroxy -2,3-dimethoxy-6-methy-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone separated from the extract of Antrodia cinnamomea, which can effectively slow down the physiological fatigue. In the invention, after 80% of maximal oxygen uptake of high-intensity exhausted exercises, the application of the compound can promote the metabolism of creatine kinase and blood ammonia in the body and contribute to the restoration of the concentration to achieve the effect of slowing down the physical fatigue.

The invention relates to the technical field of medicine, in particular to the use of rhein in the preparation of drugs for prevention and treatment of hepatic encephalopathy. The application of rheinin the preparation of drugs for preventing and treating hepatic encephalopathy is disclosed for the first time. The model of hepatic encephalopathy induced by thioacetamide (TAA) and carbon tetrachloride (CCL4) was tested repeatedly. The results showed that rhein could reduce blood ammonia, alleviate inflammation of liver and brain tissue, protect liver function, reduce liver necrosis and improveliver injury and brain injury. Therefore, rhein or its salts can be used to prepare drugs for the prevention and treatment of hepatic encephalopathy. Rhein or its salts can be used as active ingredients to prevent and cure hepatic encephalopathy, which has the advantages of obvious effect, less dosage and quick onset.

The invention discloses an anti-fatigue and sports probiotic, and belongs to the field of probiotic-related foods. The anti-fatigue and sports probiotic comprises bifidobacterium longum and BCAA, wherein the effective amount of the bifidobacterium longum combined with the BCAA is supplemented by 5 billion to 50 billion per a day, namely 5 * 109cfu/day to 5 * 1010cfu/day, and 3.2g to 4.6g of BCAA per day. The invention mainly provides application of the bifidobacterium longum combined with the BCAA in preparation of a composition for improving body composition, improving body function or reducing fatigue after sports; the improvement of the body composition comprises increasing muscle mass or reducing fat accumulation; the improvement of the body function system comprises improving athleticability; the athletic ability comprises muscle strength and muscle endurance; the reduction of the fatigue coefficient after sports is also to reduce injury or inflammation between muscle tissues after sports; the increase of the tissue glucose content after sports or the reduction of muscle injury; and the reduction of the lactic acid concentration, blood ammonia concentration or creatine phosphokinase activity in blood after sports.

The present invention relates to the field of medicines, particularly relates to new application of lycium barbarum polysaccharide, and provides application of lycium barbarum polysaccharide in preparation of drugs for reducing blood ammonia and drugs for treating hepatic encephalopathy. Experiments proof that the lycium barbarum polysaccharide can increase the survival rate of mice with hepatic encephalopathy model mice, and the lycium barbarum polysaccharide can reduce the extent of liver injury in the model mice. The lycium barbarum polysaccharide used in treating of hepatic encephalopathy is rapid in effect and good in security.

The invention discloses a derivatization gas chromatographic-mass spectrometric method for detecting the ammonia content of a biological sample. The method includes the steps: (1) detection condition;(2) sample treatment; (3) atlas analysis; (4) standard blood ammonia sample preparation; (5) content calculation. By a pre-column derivatization gas chromatography-mass spectrometry method, the ammonia content of the biological sample can be detected in a targeted manner, the method supplements a clinical examination blood ammonia spectrometry method, and the influence of examination blood coagulation conditions and hemolysis on detection results can be effectively reduced. Detection sensitivity is improved, detection steps are greatly simplified, and time and cost are saved.