Method for detecting blood ammonia content and blood ammonia diagnostic reagent kit

A diagnostic kit and blood ammonia technology, which is applied in the field of medical testing and measurement, can solve problems such as undiscovered blood ammonia measurement methods, and achieve good stability

Inactive Publication Date: 2006-03-22
王尔中
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Searching Chinese patents, only found that patent application No. 87105593.7 discloses a quick-frozen cup for blood ammonia measurement, but no ideal method for blood ammonia measurement was found

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment one (single dose)

[0086] The blood ammonia diagnostic kit of the present embodiment comprises:

[0087] Buffer 80mmol / l

[0088] Adenosine triphosphate 1mmol / l

[0089] Glutamic acid 4mmo / l

[0090] Pyruvate 4mmol / l

[0091] Ethanol 2mmol / l

[0092] Oxidized coenzyme 1mmol / l

[0093] Glutamine Synthetase 10000U / l

[0094] Pyruvate oxidase 10000U / l

[0095] Catalase 10000U / l

[0096] Aldehyde dehydrogenase 10000U / l

[0097] Stabilizer 50% (total volume)

[0098] Set on the fully automatic analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 340nm, test sub-wavelength above 405nm, the volume ratio of the blood ammonia sample to be tested is 2 / 25, and the reaction direction is positive reaction.

[0099] After the sample and reagents are added and allowed to mix, the following reactions occur:

[0100] Ammonia + glutamic acid + adenosine triphosphate glutamine synthetase Glutamine+

[0101] Ade...

Embodiment 2

[0108] Embodiment two (two doses)

[0109] The blood ammonia diagnostic reagent of the present embodiment has:

[0110] Reagent I

[0111] Buffer 100mmol / l

[0112] Glutamic acid 7mmol / l

[0113] Pyruvate 7mmol / l

[0114] Ethanol 5mmol / l

[0115] Oxidized coenzyme 2mmol / l

[0116] Glutamine Synthetase 15000U / l

[0117]Pyruvate oxidase 15000U / l

[0118] Catalase 15000U / l

[0119] Aldehyde dehydrogenase 15000U / l

[0120] Stabilizer 50% (total volume)

[0121] Reagent II

[0122] Buffer 100mmol / l

[0123] Adenosine triphosphate 3mmol / l

[0124] Stabilizer 10mmol / l

[0125] When measuring blood ammonia content, the temperature is controlled at 30°C, the reaction time is 15 minutes, the main wavelength of the test is 340nm, the secondary wavelength of the test is above 405nm, the volume ratio of the blood ammonia sample to the reagent is 1 / 25, and the reaction direction is positive reaction.

[0126] The specific measurement steps are:

[0127] Ammonia + glutamic aci...

Embodiment 3

[0135] Embodiment three (three doses)

[0136] The blood ammonia diagnostic reagent of the present embodiment is three doses, has:

[0137] Reagent I

[0138] Buffer 120mmol / l

[0139] Glutamic acid 10mmol / l

[0140] Pyruvate 10mmol / l

[0141] Ethanol 8mmol / l

[0142] Oxidized coenzyme 3mmol / l

[0143] Stabilizer 50mmol / l

[0144] Reagent II

[0145] Buffer 120mmol / l

[0146] Glutamine Synthetase 20000U / l

[0147] Pyruvate oxidase 20000U / l

[0148] Catalase 20000U / l

[0149] Aldehyde dehydrogenase 20000U / l

[0150] Stabilizer 50% (total volume)

[0151] Reagent III

[0152] Buffer 120mmol / l

[0153] Adenosine triphosphate 5mmol / l

[0154] Stabilizer 50mmol / l

[0155] Set on the automatic biochemical analyzer: temperature 25°C, reaction time 20 minutes, test main wavelength 340nm, test sub-wavelength above 405nm, the volume ratio of the measured blood ammonia sample to reagent is 1 / 50, and the reaction direction is positive reaction .

[0156] The specific mea...

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Abstract

The present invention belongs to the field of medical detection technology. The reagent kit for blood ammonia diagnosis includes buffering solution, adenosine triphophate, glutamic acid, pyruvic acid, alcohol, oxidized coenzyme, glutamine synthetase, pyruvate oxidase, hydrogen peroxidase, aldehyde dehydrogenase and stabilizer. Through mixing the sample and reagent in certain volume ratio to produce enzyme coupling reaction, and detecting in biochemical analyzer the main wavelength absorbency change speed, the blood ammonia content is measured. The present invention can obtain the measurement result in biochemical analyzer in high sensitivity, high precision and no contamination of various foreign and internal matters.

Description

technical field [0001] The invention relates to a method for measuring blood ammonia content, and at the same time, the invention also relates to a blood ammonia diagnosis kit, which belongs to the technical field of medical examination and measurement. Background technique [0002] Ammonia determination methods include micro-diffusion method, ion exchange method, enzymatic method and ammonia electrode method. The most widely used methods are enzymatic method and blood ammonia analyzer analysis method based on ion selective electrode. [0003] The diffusion method is that after the sample is alkalized, NH is released 3 , Titrate the released ammonia with acid, or use the Nessler reaction to form brown-yellow mercuric ammonium iodide for colorimetry. These methods require alkalization, which is affected by the formation of endogenous ammonia, which affects its accuracy and precision, and is rarely used at present; the ion exchange method is more accurate than the diffusion ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/31G01N21/33
Inventor 王尔中
Owner 王尔中
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