348 results about "Aldehyde dehydrogenase" patented technology

The present invention is directed to a recombinant enzymes having alcohol and aldehyde dehydrogenase activity which comprises one or more recombinant polypeptides selected from the group consisting of polypeptides which are identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and chimeric recombinant polypeptides that are a chimeric combination of at least two of the following amino acid sequences identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and functional derivatives of the polypeptides identified above which contain addition, insertion, deletion and/or substitution of one or more amino acid residues, wherein said enzymatic polypeptides have said alcohol and aldehyde dehydrogenase activity. DNA molecules encoding the recombinant polypeptides, vectors comprising such DNA molecules, host cells transformed by such vectors and processes for the production of such recombinant enzymes are provided. Furthermore, the recombinant enzymes having alcohol and aldehyde dehydrogenase activity are used for obtaining aldehydes, ketones or carboxylic acids, and specifically, 2-keto-L-gulonic acid an intermediate for the production of L-ascorbic acid (vitamin C).

The invention discloses a method for creating fragrant rice by knocking out a BADH2 (betaine aldehyde dehydrogenase) gene by utilizing a CRISPR/Cas9 system, belongs to the technical field of rice breeding, and relates to a method for creating fragrant rice by applying the rice betaine aldehyde dehydrogenase (BADH2) gene. According to the method, in the fragrant rice, the function of the BADH2 (betaine aldehyde dehydrogenase) gene is lost, so that a chemical substance of 2-acetyl-1-pyrroline(2-AP) is accumulated so as to generate the fragrance, and a sgRNA guide sequence adopted by the CRISPR/Cas9 system is any one of the sequences which are as as shown in the SEQ ID NO.2 or the SEQ ID NO.3 in the figure 1 in the specification. The method has the advantages that the BADH2 (betaine aldehydedehydrogenase) gene can be successfully knocked out, so that the fragrant rice can be quickly created; and the operation is simple, time and labor are saved, and the breeding life of the fragrant riceis shortened.

Populations of stem cells and methods for their isolation and use are provided. These stem cell populations comprise aldehyde dehydrogenase positive (ALDH^br) cells isolated from bone marrow, and ALDH^br CD105⁺ cells derived from any stem cell source. These populations may also comprise cells expressing such surface markers as CD34, CD38, CD41, CD45, CD105, CD133, CD135, CD117, and HLA-DR, and/or are substantially free from such cell surface markers as CD3, CD7, CD 10, CD 13, CD 14, C1319, CD33, CD35, CD56, CD 127, CD 138, and glycophorin A. The population may also comprise cells expressing CD90. The stem cell populations of the invention are isolated from a stem cell source such as bone marrow, peripheral blood, umbilical cord blood, and fetal liver. Methods of the invention comprise isolating and purifying stem cell populations from stem cell sources, and methods of using these cells to reconstitute, repair, and regenerate tissues.

Disclosed is a strain of Escherichia sp., capable of producing O-acetyl homoserine in high yield, with the introduction and enhancement therein of the activity of: homoserine acetyl transferase, aspartokinase and homoserine dehydrogenase; and at least one enzyme selected from a group consisting of phosphoenolpyruvate carboxylase, aspartate aminotransferase and aspartate semi-aldehyde dehydrogenase. Also, a method of producing O-acetyl homoserine using the strain is provided.

The present invention relates to methods, systems and compositions, including genetically modified microorganisms, directed to achieve decreased microbial conversion of 3-hydroxypropionic acid (3-HP) to aldehydes of 3-HP. In various embodiments this is achieved by disruption of particular aldehyde dehydrogenase genes, including multiple gene deletions. Among the specific nucleic acids that are deleted whereby the desired decreased conversion is achieved are aldA, aldB, puuC), and usg of E. coli. Genetically modified microorganisms so modified are adapted to produce 3-HP, such as by approaches described herein.

The present invention provides compounds that function as modulators of mitochondrial aldehyde dehydrogenase-2 (ALDH2) activity; and pharmaceutical compositions comprising the compounds. The present invention provides therapeutic methods involving administering a subject compound, or a subject pharmaceutical composition. The present invention further provides assays for identifying agonists of ALDH2.

A non-naturally occurring microbial organism has at least one exogenous nucleic acid encoding a MEK pathway enzyme expressed in a sufficient amount to produce MEK. The MEK pathway includes an enzyme selected from an acetoacetyl-CoA dehydrogenase (bifunctional), an acetoacetyl-CoA aldehyde dehydrogenase, a 3-oxobutyraldehyde reductase, a 3-oxobutanol dehydratase, an MEK oxidoreductase, a 3-oxobutyraldehyde aminotransferase, a 4-aminobutan-2-one deaminase, a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP aminotransferase, a 2,4-dioxopentanoate decarboxylase, an AKP deaminase, an acetylacrylate decarboxylase, an AKP decarboxylase, a glutamate dehydrogenase, a 3-oxobutyraldehyde oxidoreductase (aminating) and an AKP oxidoreductase (aminating). A 2-butanol pathway further includes an MEK reductase. A method for producing MEK or 2-butanol includes culturing these organisms under conditions and for a sufficient period of time to produce MEK or 2-butanol.

The invention relates to the table vinegar production field, in particular to a method for composite acetic acid bacterium culture and solid-state acetic acid fermentation, which solves the problems that the acetic acid bacterium is simplex in strain, the acetic acid conversion rate is low, the traditional acetic acid bacterium culture method causes instability of table vinegar quality and the like in the prior art. The method comprises the following steps: utilizing Huniang 1.01, As1.41, bacterium gluconicum, acetobacter separated from a Shanxi mature vinegar grains and high-yield acetaldehyde dehydrogenase acetobacter to form the composite acetic acid bacteria, and then carrying out continuous culture in a composite culture medium comprising grape fruit juice, multi-strain nutrition-enhancing yeast, yeast extract, ethanol and distilled water to obtain a composite acetic acid bacteria culture solution; and finally carrying out solid-state acetic acid fermentation, wherein the inoculation process adopts operating techniques of mixing the grains at a pan bottom, drawing fire, burying in fire and rubbing the grains. The invention uses the advantages of multiple strains, raises the yield of gluconic acid in metabolite, produces a final product which is moderately sour and has unique flavor, improves the conversion rate of acetic acid, and shortens the fermentation period, thereby having profound significance in the progress of the table vinegar brewage technology.

Disclosed herein is a microorganism capable of producing ethane-1,2-diol from D-xylose, and a method for producing ethane-1,2-diol using the same. More specifically, the present invention relates to an engineered Escherichia coli (E. coli) prepared by knocking out a D-xylose isomerase gene and/or an aldehyde dehydrogenase gene within the genomic DNA of E. coli and transforming an expression vector including a D-xylose dehydrogenase gene into the E. coli, and an efficient method for producing ethane-1,2-diol from D-xylose using the engineered E. coli.

The invention discloses a recombinant strain for producing a 3-hydracrylic acid homopolymer and/or a 3-hydracrylic acid copolymer and an application thereof. The construction method of the recombinant strain comprises the following steps: leading 1,3-Propanediol dehydrogenase coded genes, aldehyde dehydrogenase coded genes, 3-hydracrylic acid coenzyme A ligase coded genes and PHA (Polyhydroxyalkanoates) polymerase coded genes into a starting strain to obtain the recombinant strain. The experiments in the invention prove that the engineering bacteria can efficiently express 3-hydracrylic acid coenzyme A ligase coded genes and PHA polymerase coded genes and enable the 3-hydracrylic acid to be finally polymerized into 3-hydracrylic acid homopolymer (P(3HP)) from the 3-hydracrylic acid coenzyme A. Minitype fermentation tank experiments show that the engineering bacteria provided by the invention can have a maximum P (3HP) output of 8.9g/L after being fermented in a 6L fermentation tank and the P (3HP) can account for a maximum 91.5% of cell dry weight. In addition, the recombinant strain provided by the invention has the advantages of simple production process, low costs and broad application prospects.

A method for constructing genetic engineering bacteria used in the fermentation and the coproduction of PDO, BDO and PHP belongs to the biochemical technical field. The process of the method comprises the following steps that: D-type lactate dehydrogenase gene is removed from wild fungus used for generating PDO, and coenzyme A dependent aldehyde dehydrogenase and polyhydroxy fatty acid synthase gene are introduced so as to construct genetic engineering bacteria used in the fermentation and the coproduction of PDO, BDO and PHP; aerobic fermentation and a fermentation adjust and control mode according to which glycerol and an alkali solution undergo mixing fed batch are adopted; and a product extraction flow during which fermentation broth undergoes membrane filtration, electrodialysis, concentration and rectification steps so as to separate the products of PDO, BDO and PHP. The method has the advantages that: the constructed genetic engineering bacteria can produce PDO, BDO and PHP at the same time, thereby increasing the utilization rate of raw materials and reducing production cost; meanwhile, the synthesis of byproduct lactic acid is reduced, and an after-extraction process is simplified so as to reduce extraction cost; moreover, the method increases the synthesis of thalli NADH2 while introducing PHP.

The invention relates to preparation of a health-care product for relieving alcohol and preventing getting drunk, which prepares capsules for oral taking by using mixture of high-activity alcohol dehydrogenase and aldehyde dehydrogenase as a main ingredient, adding an enzyme stabilizer such as ferric ions, freeze-drying and adding conventional auxiliary materials. The health-care product has remarkable effects of relieving alcohol and preventing getting drunk. After a drinker takes the product before drinking, alcohol can metabolize quickly in the gastrointestinal tract into acetic acid to beabsorbed or excreted, so the damage of the alcohol to the human body is lowered.

A target substance can be produced by culturing a bacterium having an ability to produce 2-ketoglutaric acid or a derivative thereof, and an ability to produce xylonic acid from xylose, which is imparted with xylonate dehydratase activity, 2-keto-3-deoxyxylonate dehydratase activity and 2-ketoglutaric semialdehyde dehydrogenase activity, or in which these activities are enhanced, in a medium containing xylose as a carbon source to produce and accumulate the target substance in the medium, and collecting the target substance from the medium.

The invention discloses recombinant Escherichia coli and application of the recombinant Escherichia coli in synthesizing 3-hydroxypropionic acid. The recombinant Escherichia coli is formed by glycerol dehydratase genes dhaB123, glycerol dehydratase reactivating factor genes gdrA, alpha-oxoglutarate semialdehyde dehydrogenase mutant coding genes TUkgsadh and glycerol-3-phosphate dehydrogenase coding genes gpdl which are guided into host bacteria. By means of the gene knockout technique, the yield of byproduct 1,3-propylene glycol is reduced, and meanwhile, cofactors NAD+ of aldehyde dehydrogenase are regenerated. Thus, the yield of the 3-hydroxypropionic acid is improved by 1.5 times.

The invention discloses primers, a probe and a kit for detecting ALDH2 (aldehyde dehydrogenase gene2) gene polymorphism and belongs to the technical field of gene polymorphism site detection. Nucleotide sequences of the primers comprise a mutant type ARMS upstream primer represented as SEQ ID NO:1, a wild type ARMS upstream primer represented as SEQ ID NO:2 and a common downstream primer represented as SEQ ID NO:3, and the probe adopts a common Taqman probe. The kit comprises an amplification primer of an internal control gene and the Taqman probe. The primers and the probe as well as the kit comprising the primers and the probe can be used clinically for detecting various types of samples and have the remarkable advantages of high specificity, high sensitivity, short experimental period, simplicity in operation, safety, non-toxicity, low cost and the like.

The invention discloses engineering bacteria for producing trans-4-hydroxy-L-proline and a construction method and an application thereof. The construction method of the engineering bacteria provided by the invention includes the steps of A1) and A2): A1) introducing a L-proline-4-hydroxylase gene, a glutamate-5-kinase gene and a glutamate-5-semialdehyde dehydrogenase gene into a receptor cell; and A2) knocking an alpha-ketoglutaricdehydrogenase gene, an isocitratlyase gene or a proline dehydrogenase gene of the receptor cell out, or replacing a pyruvic oxidase gene of the receptor cell with an acetylcoenzyme A synthetase gene; and carrying out a reaction of the recombinant cell and a substrate to obtain the trans-4-hydroxy-L-proline. Experiments prove that the production method of the trans-4-hydroxy-L-proline can be used for production of the trans-4-hydroxy-L-proline.

The invention discloses recombinant bacteria using xylose to produce glycollic acid and a building method and application of the recombinant bacteria and belongs to the technical field of genetic engineering. The xylose dehydrogenase gene, xylonic acid lactonase gene, xylonic acid dehydratase gene, 3-deoxygenation-D-glycerin ketopentose acid aldolase gene and glycolic aldehyde dehydrogenase gene in the recombinant bacteria using the xylose to produce the glycollic acid are overexpressed. Meanwhile, the invention further provides a preparation method of the recombinant bacteria and a method using the recombinant bacteria to produce the glycollic acid. By the recombinant bacteria, the biological synthesizing path using D-xylose as the carbon source to form the glycollic acid through glycolic aldehyde conversion is achieved for the first time.

The present invention relates to methods and genetically engineered micro-organisms for the combined production of PDO, BDO, and PHP by fermentation. The micro-organism is characterized in that its D-lactate dehydrogenase gene has been deleted or functionally inactivated, and it comprises heterogenous polynucleotides encoding the Coenzyme A-dependent Aldehyde dehydrogenase and the Polyhydroxyalkanoate synthase. Methods for the construction of such micro-organisms are also disclosed

The invention discloses acetaldehyde dehydrogenase and an application thereof in an aspect of anti-alcoholic health-care products, and belongs to the technical field of enzyme engineering. The invention provides an engineering bacterium for high-efficiency expression of a human acetaldehyde dehydrogenase gene and a construction method of the engineering bacterium; the expression of the humanized acetaldehyde dehydrogenase gene, which is shown as SEQ ID NO.1, in bacillus subtilis is achieved; and an enzyme yield by fermenting the recombinant bacterium for 24h can reach 24.52U/L or above. By improving a fermentation medium, the yield of the acetaldehyde dehydrogenase is improved, and an enzyme yield of a culture medium which is added with CaCl2 salt and CuSO4 is improved to 140-180% in comparison with an original enzyme yield; therefore, a new way and a theoretical basis are provided for research and development of anti-alcoholic drugs.