Novel alcohol/aldehyde dehydrogenases

a technology of alcohol/aldehyde dehydrogenase and recombinant enzymes, which is applied in the field of recombinant enzymes, can solve the problem of no reports of cloning of such genes so far
US20050090645A1Inactive Publication Date: 2005-04-28ROCHE VITAMINS INC
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
ROCHE VITAMINS INC
Publication Date
2005-04-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention is directed to a recombinant enzymes having alcohol and aldehyde dehydrogenase activity which comprises one or more recombinant polypeptides selected from the group consisting of polypeptides which are identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and chimeric recombinant polypeptides that are a chimeric combination of at least two of the following amino acid sequences identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and functional derivatives of the polypeptides identified above which contain addition, insertion, deletion and / or substitution of one or more amino acid residues, wherein said enzymatic polypeptides have said alcohol and aldehyde dehydrogenase activity. DNA molecules encoding the recombinant polypeptides, vectors comprising such DNA molecules, host cells transformed by such vectors and processes for the production of such recombinant enzymes are provided. Furthermore, the recombinant enzymes having alcohol and aldehyde dehydrogenase activity are used for obtaining aldehydes, ketones or carboxylic acids, and specifically, 2-keto-L-gulonic acid an intermediate for the production of L-ascorbic acid (vitamin C).
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Description

FIELD OF THE INVENTION

[0001] The present invention relates to recombinant enzymes, particularly, novel recombinant alcohol / aldehyde dehydrogenases (hereinafter referred to as AADH or AADHs) having alcohol and aldehyde dehydrogenase activity. The present invention also relates to novel recombinant DNA molecules encoding AADHs, recombinant expression vectors containing said DNAs, and recombinant organisms containing said recombinant DNA molecules and / or said recombinant expression vectors. Furthermore, the present invention relates to a process for producing recombinant AADHs and a process for producing aldehydes, carboxylic acids and ketones, especially, 2-keto-L-gulonic acid (herein after referred to as 2KGA) by utilizing said recombinant enzymes, and a process for producing aldehydes, carboxylic acids and ketones, especially, 2KGA by utilizing said recombinant organisms. BACKGROUND OF THE INVENTION

[0002] 2-KGA is an important intermediate for the production of L-ascorbic acid (vi...

Claims

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