56 results about "Nalidixic acid" patented technology

The invention discloses a complex enrichment medium used for salmonella, listeria monocytogenes and staphylococcus aureus and a method for preparing the same. The complex enrichment medium comprises the following components in weight portion: 15 to 19 portions of tryptone, 2 to 4 portions of peptone, 2 to 3 portions of sodium dihydrogen phosphate, 2 to 3 portions of glucose, 0.5 to 1.5 portions of aesculin, 1, 000 portions of distilled water, 1 to 2.5 portions of sodium pyruvate, 3 to 10.0 portions of mannitol, 1.0 to 25.0 portions of sodium chloride, 1 to 2.5 portions of lithium chloride, 0.0001 to 0.0002 portion of potassium tellurite, and 0.005-0.015 portion of nalidixic acid, wherein the pH value is between 7.1 and 7.5. The complex enrichment medium can inhibit the growth of other pathogenic microorganisms while performing enrichment on three target pathogens, thus the complex enrichment medium can be directly applied to the isolation culture and the biological assay test of target bacteria, and can also be directly applied to a detection technology of a plurality of pathogens based on one detection platform for multiple PCR and the like to make a diagnostic report.

The present invention provides methods and compositions for preventing or inhibiting human food-borne pathogens in animals, and methods for increasing feed efficiency in animals by administering to the animal effective amounts of probiotic lactic acid producing bacteria. Further provided are feed compositions comprising probiotic lactic acid producing bacteria. A preferred probiotic lactic acid producing bacteria is Lactobacillus acidophilus strain ATCC accession number PTA-5249. This bacterial strain inhibits nalidixic acid-resistant Escherichia coli 0157:H7.

The invention provides a group B streptococcus selective culture medium and a using method thereof, and belongs to the field of microbial culture media. 1 liter of the culture medium comprises the following components: 1 to 10 grams of heart extract, 1 to 50 grams of new peptone, 1 to 10 grams of dextroglucose, 1 to 10 grams of NaCl, 0.1 to 5 grams of Na2HPO4, 1 to 10 grams of Na2CO3, 1 to 50 milligrams of gentamycin, 1 to 50 milligrams of nalidixic acid and the balance of water. The invention also provides a using method for the culture medium. The invention has the advantages that: the group B streptococcus selective culture medium provided by the invention can inhibit the growth of other bacteria, and if a sample has group B streptococcus, the streptococcus can be normally grown to predominant bacteria so that the culture medium brings great convenience to the subsequent identification operation and improves the detection accuracy and the detection efficiency of the group B streptococcus; and the using method for the selective culture medium is simple in operation and does not need special instruments and equipment.

The invention provides a culture medium used for separating and screening lactic acid bacteria. The formula of the culture medium comprises the following components: relative to the dosage of 1000ml of a solvent, 5.0-15.0g of tryptone, 5.0-15.0g of beef extract, 2.0-10.0g of yeast extract, 1.0-3.0g of diammonium citrate, 15.0-25.0g of glucose, 0.2-2.0 mL of Tween-80, 2.0-8.0g of sodium acetate, 15.0-20.0g of agar, 2.0-30.0g of calcium carbonate, a compound acid-base indicator containing 0.01-0.5g of methyl red and 0.01-0.7g of bromothymol blue, 0.5-5.0g of ascorbic acid, 0.1-0.5g of L-cysteine HCl, and a selective antibiotic composed of 250000-500000 IU of polymyxin B and 0.01-0.05g of nalidixic acid, wherein the pH value of the culture medium is 6.8; and the solvent is composed of a primary solvent and a secondary solvent, the primary solvent is distilled water or aged seawater, and the secondary solvent is saline solution. The culture medium has the characteristics of being high in accuracy, obvious in authentication phenomenon and the like during separation and screening for lactic acid bacteria.

The invention relates to an enrichment culture solution for the detection of Salmonella in poultry eggs, belonging to the technical field of the enrichment culture solution which can realize the selective enrichment of sublethal injured Salmonella of which amount is extremely low in poultry eggs. 1L of the enrichment culture solution comprises the following raw material components by weight: 7-11g of peptone, 5-8g of sodium chloride, 7-9g of disodium hydrogen phosphate, 0.5-1g of potassium dihydrogen phosphate, 6-8mg of brilliant green, 0.5-1g of bile salt, 10-14mg of nalidixic acid and 15-40g of skimmed milk powder. The enrichment culture solution can realize the selective enrichment of sublethal injured Salmonella of which amount is extremely low; and the enrichment processing steps of Salmonella in foods are reduced, the defect that the leak detection of a small amount of sublethal injured Salmonella in a sample can be overcome and the detection reliability and accuracy of Salmonella in foods can be increased.

The invention provides a combination bacteriostat for selectively culturing HP (helicobacter pylori), the formula of the combination bacteriostat is that: 3-50mg / l of vancomycin, 5-10.0mg / l of nalidixic acid, 3-5.0mg / l of TMP, 1-2.0mg / l of amphotericin and 8-10mg / l of Nisin. The preparation method of the combination bacteriostat for selectively culturing the HP mainly comprises the following procedures of culture medium preparation and bacteria inoculation, culture and preservation. According to the combination bacteriostat containing the amphotericin B, the nalidixic acid, the vancomycin, the TMP and the Nisin, through the addition of the combination bacteriostat in a culture medium, not only can the pollution of mixed bacteria such as bacillus subtilis, gram positive cocci and mucedine be reduce, but also thymidine needed by the growth of the HP can be provided for the HP to inhibit the growth of the mixed bacteria.

The invention discloses a method for screening a drug which is resistant to pan-drug-resistant acinetobacter baumannii by using caenorhabditis elegans. First, a caenorhabditis elegans-pan-drug-resistant acinetobacter baumannii infection model used for screening the efficacy of a composition is constructed, wherein caenorhabditis elegans has double gene mutation of glp-4 and sek-1, the culture medium of the caenorhabditis elegans-pan-drug-resistant acinetobacter baumannii co-culture consists of 20% of BHI, 5-20[mu]m of nalidixic acid and 5-20[mu]g / ml of FeCl3; the concentration of the pan-drug-resistant acinetobacter baumannii is 1*10<6>-1*10<9>CFU / mL; the duration time of the bacterial infection on the caenorhabditis elegans is 6-12 hours; and the time of the treatment on an infected modelby a drug is 24-48 hours. The model can be used for fast and high-throughput screening of in-vivo antibacterial activity of various compounds or drugs or compositions, and compared with an in-vivo animal infection model, the model has the huge advantages of low preparation cost, short period and easy operation. Compared with an in vitro model, the model can be used to screen out compounds which have large in-vivo toxicity, poor metabolism and low in vitro-in vivo correlation.

The invention relates to the technical field of detection of pathogenic bacterium, and discloses a selective medium for multiplex proliferation of salmonella, listeria monocytogenes and vibrio parahaemolyticus. The selective medium is prepared from tryptone, peptone, sodium chloride, sodium dihydrogen phosphate, glucose, mannitol, esculin hydrate, sodium citrate, skim milk powder, sterile purified water, potassium tellurite solution, acriflavine solution and nalidixic acid solution. A preparation method of the selective medium comprises the following steps: preparation of potassium tellurite solution; preparation of acriflavine solution; preparation of nalidixic acid solution; preparation of a semi-finished product medium; addition of a sample; and addition of potassium tellurite solution, acriflavine solution and nalidixic acid solution in the medium. The selective medium can carry out multiplex proliferation on the target bacteria and inhibit non-target bacteria, is small in inhibiting effect on the target bacteria in the sub-lethal state so that the target bacteria in the sub-lethal state can realize effective proliferation. The preparation method of the selective medium is easy to operate and high in efficiency.

The present invention relates to live attenuated Salmonella cultures for use as vaccines. The Salmonella cultures of the present invention have a substantially reduced capacity to grow and replicate in the presence of bile. The reduced capacity for growth is due to a metabolic-drift mutation induced by exposure to a combination of nalidixic acid and rifampicin for a time and under conditions sufficient to induce the mutation.

Disclosed is a 3-substituted nalidixic acid analog compound and its preparation method and uses in pharmacy, wherein the compound has antineoplastic activity and a general formula (I) with the 3-position substituted by benzimidazole and benzothiazole, the preparation process of the compound comprises condensing various substituted naphthyridine ketone-3-formylic acid, o-phenylenediamine, o-aminophenol, o-aminobenzenethiol and into polyphosphoric acids. The compound can be used in preparing anti-cancer medicaments.

The invention relates to the technical field of pseudomonas aeruginosa detection and discloses a pseudomonas aeruginosa chromogenic culture medium and a method for quick detection by the same. The pseudomonas aeruginosa chromogenic culture medium comprises a basic medium, a chromogenic substrate and an antibacterial agent. The chromogenic substrate comprises 2,3,5-phenyl tetrazolium chloride, 4-methyl umbelliferone-D-glucuronide, and the antibacterial agent comprises nalidixic acid, cycloserine and bile salt. 2,3,5-phenyl tetrazolium chloride acts on a pseudomonas aeruginosa metabolite to showa red color; 4-methyl umbelliferone-D-glucuronide acts on pseudomonas aeruginosa to show fluorescence under a 366nm ultraviolet lamp; by joint action of 2,3,5-phenyl tetrazolium chloride and the antibacterial agent, growth of common disturbance bacteria can be inhibited, and false positive results are avoided. By adoption of the pseudomonas aeruginosa quick detection method, detection and count of pseudomonas aeruginosa can be finished in 24h, and time saving, labor saving, stability, reliability and high sensitivity are realized.

The invention belongs to methods for killing bacteria and fungi in chlamydomonas reinhardtii and discloses a method for sterilization in a chlamydomonas reinhardtii culture process by mixture of tebuconazole and nalidixic acid. The method includes: A, inoculating chlamydomonas contaminated by mixed bacteria onto a TAP solid medium plate, and culturing for 3-5 days at the temperature of 23+ / -0.5 DEG C, in an occulting light period of 14 / 10 and under the light intensity of 8000Lx; C, transferring the chlamydomonas well grown on the TAP solid medium plate to a TAP plate with mixed antibacterial agents, and culturing for 5-8 days at the temperature of 23+ / -0.5 DEG C, in the occulting light period of 14 / 10 and under the light intensity of 8000Lx; D, transferring the sterilized chlamydomonas to the common TAP solid medium plate, and culturing for 3-5 days at the temperature of 23+ / -0.5 DEG C, in the occulting light period of 14 / 10 and under the light intensity of 8000Lx to achieve a standby for subsequent experiments or breed conservation. The method has the advantage that the problem of bacterial contamination in scientific experiment and application process of chlamydomonas is solved by adoption of the mixed antibacterial agents for treating bacterial and fungus contamination of chlamydomonas.

The invention discloses a complex enrichment medium used for salmonella, listeria monocytogenes and staphylococcus aureus and a method for preparing the same. The complex enrichment medium comprises the following components in weight portion: 15 to 19 portions of tryptone, 2 to 4 portions of peptone, 2 to 3 portions of sodium dihydrogen phosphate, 2 to 3 portions of glucose, 0.5 to 1.5 portions of aesculin, 1, 000 portions of distilled water, 1 to 2.5 portions of sodium pyruvate, 3 to 10.0 portions of mannitol, 1.0 to 25.0 portions of sodium chloride, 1 to 2.5 portions of lithium chloride, 0.0001 to 0.0002 portion of potassium tellurite, and 0.005-0.015 portion of nalidixic acid, wherein the pH value is between 7.1 and 7.5. The complex enrichment medium can inhibit the growth of other pathogenic microorganisms while performing enrichment on three target pathogens, thus the complex enrichment medium can be directly applied to the isolation culture and the biological assay test of target bacteria, and can also be directly applied to a detection technology of a plurality of pathogens based on one detection platform for multiple PCR and the like to make a diagnostic report.

The invention discloses an application of citral in inhibiting growth of multi-drug resistant enterobacter cloacae. The citral can inhibit growth of multi-drug resistant enterobacter cloacae as the citral has relatively good in vitro killing action to multi-drug resistant enterobacter cloacae resisting cefazolin, cefotaxime, augmentin, meropenem, ofloxacin, levofloxacin, ciprofloxacin, cefoxitin, minocyline, imipenem, piperacillin, azithromycin, macrodantin, sulfamethoxazole and nalidixic acid. The minimum bactericidal concentration is 1.6 mg / mL and the minimal inhibitory concentration is 1.0 mg / mL. The invention provides inhibiting action of citral to multi-drug resistant enterobacter cloacae and the citral has wide application value in the field of medicine.

The invention discloses a nocardia asteroid selective medium for a sputum specimen, and belongs to the field of inspective microorganism culture. The nocardia asteroid selective medium is characterized in that 1000ml of the medium comprises beef liver powder, malt extract powder, yeast extract powder, sodium chloride, agar, ferroporphyrin, calcium lactate, nalidixic acid, argutone, vitamin K1, sterile defibered sheep blood and the balance of distilled water. Compared with the prior art, the nocardia asteroid selective medium has the characteristics of reliable detection and high nocardia asteroid separation rate.

The invention discloses a western medicine composition for treating chronic bronchitis. The western medicine composition for treating chronic bronchitis is prepared from the following main raw materials in parts by weight: 4-9 parts of tenacissoside H, 3-10 parts of pristimerin, 7-15 parts of nalidixic acid, 2-6 parts of trihydroxyflavone, 12-17 parts of benzoylpaeoniflorin, 8-9 parts of picroside, 1-4 parts of myricetin, 0.7-1.5 parts of andrographolide and 0.1-0.5 part of salbutamol sulfate. According to the western medicine composition disclosed by the invention, by virtue of synergistic effects of all the medicines, the aim of comprehensive rehabilitation is achieved, both symptoms and root causes are treated, the treatment course is short, the effect can be taken quickly, toxic and side effects are less, and the cost is extremely low.

The invention discloses application of sanguinarine in inhibiting growth of multi-drug-resistant enterobacter horbiae. The sanguinarine has a good in-vitro killing effect on the multi-drug-resistant enterobacter horbiae of piperacillin, levofloxacin, bromgrantine, imipenem, ofloxacin, furadantin, cefathiaquine, azithromycin and naphthyridine acid, and can inhibit the growth of the multi-drug-resistant enterobacter horbiae, the minimum inhibitory concentration is 0.12 mg / mL, and the minimum bactericidal concentration is 0.24 mg / mL. The invention provides the inhibition effect of the sanguinarine on the multi-drug-resistant enterobacter horbiae, and the sanguinarine has wide application value in the fields of medicines and the like.

The invention discloses a nocardia asteroid selective medium for sputum samples and belongs to the field of detection of microculture. The nocardia asteroid selective medium is characterized in that 1000 ml of culture medium comprises ox liver powder, malt extract powder, yeast extract powder, sodium chloride, agar, ferroporphyrin, calcium lactate, nalidixic acid, Incarvillea argute ketone, vitamin K1, casein phosphopeptides and sterile defiber sheep blood and the balance of distilled water. Compared with the prior art, the nocardia asteroid selective medium has the advantages of reliability in detection and high separation efficiency of nocardia asteroid.

The invention discloses a culture medium used for performing selective separation on pathogenic listeria. The culture medium comprises base components including tryptone, peptone, beef extract powder, sodium chloride and disodium hydrogen phosphate, the culture medium also comprises beta-D-allose; more preferably, the culture medium also comprises acid trypaflavine, nalidixic acid and lithium chloride. Compared with a traditional enriched culture medium, the culture medium provided by the invention is increased in sensitivity by 16 percent when separating the pathogenic listeria, and a culture step is reduced, and 48 hours of culture time is shortened.

The present invention relates to an opening type culture method of Paracoccus FSTB-2, wherein a bactericide is poured during the opening type culture process of Paracoccus FSTB-2, the Paracoccus sp. FSTB-2 is preserved in the China General Microbiological Culture Collection Center on June 1, 2015 and has a preservation number of CGMCC No.10938, and the bactericide is one or a plurality of materials selected from lincomycin, rifamycin SV, nalidixic acid, guanidine hydrochloride, and the like. According to the present invention, according to the bacterium characteristic of Paracoccus FSTB-2, the specific bactericide is poured during the opening type culture process of Paracoccus FSTB-2 by using the salt-containing and COD-containing wastewater, such that the bacterial contamination can be significantly reduced, and the Paracoccus FSTB-2 growth and reproduction and the wastewater treatment effect cannot be affected.

The invention belongs to the technical field of microbes and discloses a specific solid streptococcus culture medium, a preparation method and application thereof. The specific solid streptococcus culture medium contains 10 g / L of peptone, 5 g / L of tryptone, 5 g / L of a yeast extract, 15 g / L of an agar powder, 2.5 g / L of KCl, 0.06 g / L of urea, 0.1742 g / L of arginine, 20 mg / L of nalidixic acid and any mass / volume ratio of monosaccharide according to the mass / volume ratio. The nalidixic acid is added into the culture medium so that the problem of bacterial infection easily occurring in the experimental operation process can be solved, and meanwhile the solid culture medium can also be used for screening, identifying, separating and purifying streptococci and the like.

The invention relates to a fast-growing solid culture medium of tubercle bacillus, comprising solution A and solution B, wherein the A solution includes: 1.0-2.0g of monopotassium phosphate, 0.3-0.5g of magnesium sulfate, 0.5-0.8g of magnesium citrate, 2.0-3.0g of asparagine, 15-20g of agar powder and 800ml of deionized water; the solution B comprises: 3.0-4.0g of bovine serum albumin, 4-5g of glucose, 1.2-1.8g of catalase, 5-10g of oleic acid, 0.01-0.10g of triphosadenine, 0.3-0.8g of coenzyme A, 0.03-0.09g of cytochrome C, 0.06-0.10g of Alpha-naphthylacetic acid, 600Mu of polymyxin B, 60Mug of amphotercin B, 240Mug of nalidixic acid, 60Mug of trimethoprim, 60Mug of azlocillin, and 200ml of deionized water; more preferably, the A solution also comprises 0.02-0.03g of ammonium ferric citrate, edible gourmet powder for replacing asparagine and human or animal strum for replacing bovine serum albumin; the invention also provides a standardized production method of the fast-growing solid culture medium. The fast-growing solid culture medium of the tubercle bacillus has smart design, simple and convenient preparation, low cost, stable and reliable stable, easy standardized large-scale production and fast-growing tubercle bacillus cultured on the medium, thereby ensuring a drug sensitive test to be fast and accurate and being good for early diagnosis and treatment of the tubercle bacillus.

The invention discloses a medicine for treating chicken paratyphoid and a preparation method of the medicine. The medicine is prepared from composition A and composition B, wherein the composition A is prepared from the following raw materials: mequindox, ciprofloxacin, nalidixic acid sodium salt, gentamicin, sulfamethazine and chloramphenicol; the composition B is prepared from the following raw materials: radix scutellariae, cortex phellodendri, herba plantaginis, dioscorea opposite, pericarpium papaveris, dendrobium officinale, rhizoma zingiberis, flos lonicerae, fructus forsythia, folium isatidis, radix isatidis, herba taraxaci, herba patriniae, rhizoma belamcandae, herba houttuyniae, rhizoma atractylodis and glucose. The medicine has the advantages that few antibiotics and chemical medicines are contained, administration is flexible, illness containment and chicken physical condition nursing are performed simultaneously, use effect is remarkable, the cost is low, and chickens can be helped to recover quickly.

The invention provides a group B streptococcus enrichment broth and application thereof, and relates to the technical field of microorganism cultivation. The group B streptococcal enrichment broth includes Todd-Hewitt broth, nalidixic acid and polymyxin B; wherein the concentration of nalidixic acid is 28-32 μg / mL; the concentration of polymyxin B is 8-12 μg / mL. The Group B Streptococcus enrichment broth can increase the quantity of Group B Streptococcus in the inoculum, inhibit the growth of miscellaneous bacteria, and improve the detection rate of Group B Streptococcus.

The invention discloses application of luteolin in inhibiting the growth of multi-drug resistant enterobacter cloacae. Luteolin has a good in-vitro killing effect on multi-drug resistant enterobactercloacae with resistance to cefazolin, cefotaxime, augmentin, meropenem, ofloxacin, levofloxacin, ciprofloxacin, cefoxitin, minocycline, imipenem, piperacillin, azithromycin, furantoin, sulfamethoxazole and nalidixic acid, can inhibit the growth of the multi-drug resistant enterobacter cloacae, and has a minimum bactericidal concentration of 0.5mg / mL and a minimum inhibitory concentration of 0.3mg / mL. The invention provides the inhibition effect of luteolin on the multi-drug resistant enterobacter cloacae, and has wide application value in the fields of medicine and the like.

The invention provides a special culture medium for streptococcus for producing sodium hyaluronate and a preparation method of the special culture medium. The culture medium is prepared from the following components (by weight): 30-9 parts of white granulated sugar, 8-14 parts of yeast extract powder, 2.5-4 parts of buffer inorganic salt, 14-28 parts of L-arginine, 10-30 parts of a polyether defoamer, 2.5-5 parts of wheat bran, 1.5-2.8 parts of inorganic salt of trace elements, 0.5-1.8 parts of gentamicin, 0.5-1.8 parts of nalidixic acid and 40-60 parts of water. According to the preparation method of the special culture medium for streptococcus for producing sodium hyaluronate, a technical means of rational combination of L-arginine, wheat bran and inorganic salts of trace elements is adopted and gentamicin and nalidixic acid are added, so that the probability of adverse reaction is reduced; the yield of the prepared sodium hyaluronate can reach 6.7 g / L-7.2 g / L, the bottleneck of technical development of the sodium hyaluronate industry is broken through, and the production cost is reduced. The special culture medium has a good application prospect.

The invention discloses an osmanthus fragrans root sterilization cultivation fertilizer and a preparation method thereof. The osmanthus fragrans root sterilization cultivation fertilizer is prepared from the following raw materials in parts by weight: 28-40 parts of kieselguhr, 25-30 parts of plant ash, 12-28 parts of almond dregs, 30-35 parts of sesame dregs, 26-30 parts of astragalus smicus straw, 4-12 parts of moss, 4-8 parts of willow bark, 1-2 parts of glutamic acid, 1-3 parts of indolebutyric acid, 3-5 parts of glucose, 4-6 parts of sodium nitrate, 1-2 parts of cephalosporin C, 2-3 parts of nalidixic acid, 2-4 parts of bactericide silicon imidazole prochloraz, 2-4 parts of a bordeaux mixture, 10-15 parts of carbon residues and a proper amount of water. The willow bark is degraded so as to generate saligenin, and together with the cephalosporin C, the nalidixic acid, the bactericide silicon imidazole prochloraz and the bordeaux mixture, the growing environment around the root of osmanthus fragrans can be sterilized, meanwhile root protection and generation effects can be achieved, and the planting survival rate can be increased.

The invention relates to a preparation method of nalidixic acid. The preparation method is characterized in that at least three intermediates are adopted in preparation, including a first intermediate namely methyl-2-pyridyl)amino]methylene}diethyl malonate, a second intermediate namely ethyl, 4hydro-7-methyl-1,8-naphthyridine-3-carbonate, and a third intermediate namely ethyl-1,4-dihydro-7-methyl-4-oxy-1,8-naphthyridine-3-carbonate; and a relatively low temperature is adopted during preparation of the second intermediate, and bromoethane is adopted during preparation of the third intermediate. Therefore, the reaction time is saved, and the cost of reactants is reduced. More importantly, impurities are reduced as much as possible to improve the quality of a finished product.