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Special culture medium for streptococcus for producing sodium hyaluronate and preparation method thereof

A technology of sodium hyaluronate and culture medium, applied in the biological field, can solve problems such as the inability to meet industrial production needs, achieve good application prospects, break through the bottleneck of technological development, and reduce production costs.

Pending Publication Date: 2019-11-22
SHANGHAI JINGFENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the output of sodium hyaluronate produced by existing methods is generally only 6g / L, which cannot meet the growing demand for industrial production

Method used

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  • Special culture medium for streptococcus for producing sodium hyaluronate and preparation method thereof
  • Special culture medium for streptococcus for producing sodium hyaluronate and preparation method thereof
  • Special culture medium for streptococcus for producing sodium hyaluronate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A streptococcus special culture medium for producing sodium hyaluronate, in parts by weight, comprises the following components:

[0033]

[0034] The preparation method of the streptococcus special medium for producing sodium hyaluronate comprises the following steps:

[0035] (1) Weigh each component according to the ingredient list, dissolve white sugar, yeast extract powder and wheat bran in water, and mix at 20°C for 20 minutes to obtain the first mixed solution;

[0036] (2) dissolving buffering inorganic salts, inorganic salts of trace elements, L-arginine, gentamicin, and nalidixic acid in water, and mixing at 50° C. for 10 minutes to obtain a second mixed solution;

[0037] (3) Mix the first mixed solution, the second mixed solution and the polyether antifoaming agent at 30° C. for 5 minutes to a clear state to obtain the culture medium.

Embodiment 2

[0039] A streptococcus special culture medium for producing sodium hyaluronate, in parts by weight, comprises the following components:

[0040]

[0041] The preparation method of the streptococcus special medium for producing sodium hyaluronate comprises the following steps:

[0042] (1) Weigh each component according to the ingredient list, dissolve white sugar, yeast extract powder and wheat bran in water, and mix at 40°C for 50 minutes to obtain the first mixed solution;

[0043] (2) Dissolving buffer inorganic salts, inorganic salts of trace elements, L-arginine, gentamicin, and nalidixic acid in water, and mixing at 70° C. for 22 minutes to obtain a second mixed solution;

[0044] (3) Mix the first mixed solution, the second mixed solution, and the polyether antifoaming agent at 33° C. for 14 minutes to a clear state to obtain the culture medium.

Embodiment 3

[0046] A streptococcus special culture medium for producing sodium hyaluronate, in parts by weight, comprises the following components:

[0047]

[0048]

[0049] The preparation method of the streptococcus special medium for producing sodium hyaluronate comprises the following steps:

[0050] (1) Weigh each component according to the ingredient list, dissolve white sugar, yeast extract powder and wheat bran in water, and mix at 20°C for 20 minutes to obtain the first mixed solution;

[0051] (2) dissolving buffering inorganic salts, inorganic salts of trace elements, L-arginine, gentamicin, and nalidixic acid in water, and mixing at 50° C. for 10 minutes to obtain a second mixed solution;

[0052] (3) Mix the first mixed solution, the second mixed solution and the polyether antifoaming agent at 30° C. for 5 minutes to a clear state to obtain the culture medium.

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Abstract

The invention provides a special culture medium for streptococcus for producing sodium hyaluronate and a preparation method of the special culture medium. The culture medium is prepared from the following components (by weight): 30-9 parts of white granulated sugar, 8-14 parts of yeast extract powder, 2.5-4 parts of buffer inorganic salt, 14-28 parts of L-arginine, 10-30 parts of a polyether defoamer, 2.5-5 parts of wheat bran, 1.5-2.8 parts of inorganic salt of trace elements, 0.5-1.8 parts of gentamicin, 0.5-1.8 parts of nalidixic acid and 40-60 parts of water. According to the preparation method of the special culture medium for streptococcus for producing sodium hyaluronate, a technical means of rational combination of L-arginine, wheat bran and inorganic salts of trace elements is adopted and gentamicin and nalidixic acid are added, so that the probability of adverse reaction is reduced; the yield of the prepared sodium hyaluronate can reach 6.7 g / L-7.2 g / L, the bottleneck of technical development of the sodium hyaluronate industry is broken through, and the production cost is reduced. The special culture medium has a good application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a streptococcus special culture medium for producing sodium hyaluronate and a preparation method thereof. Background technique [0002] Sodium hyaluronate is an acidic mucopolysaccharide, the sodium salt of glycosaminoglycan composed of D-glucuronic acid and N-acetyl-D-glucosamine disaccharide units. It can be used in various ophthalmic operations, treatment of arthritis and acceleration of wound healing and other auxiliary drugs; it can be used in cosmetic creams, masks, shampoos and other products to maintain moisture in the skin and hair, and moisturize the skin and hair; it can Applied in contact lens care products, it can moisturize and lubricate and increase water retention. Sodium hyaluronate can be used as one of the raw materials of cosmetics (emulsion, lotion, etc.), contact lens care products, eye drops, etc. The initial method of obtaining sodium hyaluronate is mainly t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26C12N1/20C12R1/46
CPCC12P19/26C12N1/20
Inventor 陈仁爱杨永超刘昕徐宗民杨昌盛
Owner SHANGHAI JINGFENG PHARMA
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