60 results about "Gentamicin" patented technology

The invention provides a photodynamic and chemotherapy synergistic antibacterial composite material, the composite material comprises an intermediate composite material and hyaluronic acid HA coating the outer side of the intermediate composite material, and the intermediate composite material takes a photosensitizer i-ZnPPOPs as a substrate and is loaded with calcium peroxide and gentamicin. The composite material has excellent bacteria targeting characteristic and antibacterial effect. Specifically, hyaluronic acid can target hyaluronidase secreted by bacteria so as to capture the bacteria, and then the intermediate composite material is transferred to the surfaces of the bacteria and interacts with negatively charged bacterial membranes. Then, the exposed biomass-based CaO2 is released and reacts with water to generate H2O2 and O2, so that oxygen deficit of the infected wound surface is relieved, generation of < 1 > O2 in the i-ZnPPOPs is effectively promoted, and the photodynamic antibacterial effect is further improved. Meanwhile, the released gentamicin can further improve the antibacterial efficiency of the chemotherapy.

The invention discloses a three-dimensional multi-layer flaky nano-enzyme, a preparation method thereof and an application of the three-dimensional multi-layer flaky nano-enzyme in immunochromatography detection of micromolecular environmental pollutants, and belongs to the technical field of nano-enzymes and immunochromatography detection. The three-dimensional flaky nano enzyme is elaborately designed by continuously constructing Au nano particles (NP) and AuPt bimetallic NP on a flexible graphene oxide (GO) nano sheet. The nano-enzyme has the beneficial effects that in the nano-enzyme, the two-dimensional GO provides a huge surface area and high stability, hundreds of large AuNPs provide enhanced colorimetric ability and a large-area spherical surface, and tens of thousands of AuPt satellites serve as space catalytic sites and generate excellent peroxidase-like activity. The dual-mode ICA provided by the invention can be used for simultaneously detecting three important drugs, namely gentamicin, clenbuterol and ractopamine, in a real complex sample, and the detection performance of the existing colorimetric ICA technology based on simple catalysis is greatly improved, so that the dual-mode ICA shows huge application potential in the aspect of monitoring small molecular targets in real time under different conditions.

The invention belongs to the technical field of biology, and particularly relates to bifidobacterium longum subsp.infantis FMBL B250020GXY for metabolizing breast milk oligosaccharide and application of the bifidobacterium longum subsp.infantis FMBL B250020GXY, the bifidobacterium longum subsp.infantis FMBL B250020GXY is preserved in China Center for Type Culture Collection on September 1, 2025, and the preservation number is CCTCC M 20251931; three kinds of breast milk oligosaccharides, namely 2 '-fucosyllactose (2'-FL), lactose-N-neotetraose (LNnT) and lactose-N-tetraose (LNT), can be metabolized; plant source carbohydrates such as fructo-oligosaccharide and synanthrin can be metabolized; the compound has a relatively strong capability of removing DPPH and ABTS free radicals; a good inhibition effect is achieved on common pathogenic bacteria; good tolerance to acid and cholate is achieved; the strain is sensitive to gentamicin, streptomycin, tetracycline, chloramphenicol and vancomycin; the compound can be used for preparing antioxidant products, medicines for inhibiting pathogenic bacteria, infant foods and fermented foods, and has a wide application prospect.

The invention discloses a Weissella virida 38-3 strain and an application thereof. Relates to the technical field of microorganisms. The invention provides a strain and application thereof. The strain is safe in separation source, high in growth speed and excellent in fermentation performance; the strain has the advantages of good biological safety, no hemolytic activity and no generation of bioamine, is sensitive to antibiotics such as ampicillin, erythromycin, kanamycin, streptomycin, tetracycline, vancomycin, chloramphenicol, gentamicin, clindamycin, ciprofloxacin, cefixime and the like, has good probiotic performance, has good gastric juice and intestinal juice tolerance and strong cell surface adhesion, and can be used for preparing antibiotics. Good pathogenic bacterium inhibiting and cholesterol lowering capabilities are realized. The strain disclosed by the invention can be used as a probiotic to prepare a food leavening agent, multifunctional medicines, functional foods or health-care foods, and has wide market prospects and application values.

The present invention discloses an IS26-based resistance gene control plasmid, engineering bacteria, bacterial agent and application, belonging to the technical field of genetic engineering. The nucleotide sequence of the IS26-based resistance gene control plasmid is shown in SEQ ID NO: 1 or SEQ ID NO: 2. The engineering bacteria and bacterial agent containing the resistance gene control plasmid can be used for the control of resistance genes. By specifically destroying the insertion sequence IS26, the number of resistance genes can be fundamentally reduced and the spread of resistance genes can be blocked, providing a precise solution for the treatment of drug resistance. It has broad-spectrum resistance bacteria control performance and can effectively control gentamicin, tetracycline, chloramphenicol and ampicillin-resistant bacteria in wastewater, with the highest control efficiency reaching 83.76%. The plasmid, engineering bacteria and bacterial agent have low preparation costs, can be used for in-situ treatment of wastewater, have low treatment costs and good effects, and have broad application prospects.

The invention belongs to the technical field of veterinary drugs, and discloses a Chinese pulsatilla root alcohol extract and gentamicin composition and application thereof in inhibition of drug-resistant salmonella typhimurium. The pulsatilla alcohol extract composition provided by the invention comprises pulsatilla alcohol extract and gentamicin, and for salmonella typhimurium, the optimal ratio concentration of the pulsatilla alcohol extract to the gentamicin is 31.25 mg / mL: 2 [mu] g / ml; the Chinese pulsatilla root alcohol extract and the gentamicin have the synergistic bacteriostatic effect on salmonella typhimurium, the composition reduces the concentration of single bacteriostasis of the Chinese pulsatilla root alcohol extract and the gentamicin, the cost is saved, and the composition has wide application prospects and development value in the fields of food processing, medicine and breeding industry.

The application discloses application of antibacterial peptide BMAP-28 and antibiotics in combined preparation of antibacterial drugs and belongs to the technical field of biological medicine. The antibiotics are selected from one or more of the following: ciprofloxacin, gentamicin and cefixime. The application combines the antibacterial peptide BMAP-28 with antibiotics such as ciprofloxacin and gentamicin, finds that the combination has a significant synergistic antibacterial effect on Enterobacter sakazii, can greatly save the dosage of the drug, reduce the toxic side effect and block the evolution process of drug resistance. Through the destruction of the bacterial cell membrane and the assistance of the antibiotic effect, the combination can effectively overcome the multiple drug resistance, widen the antibacterial spectrum and has a wide clinical application prospect.

The invention discloses an anti-GM antibody and application thereof, and relates to the technical field of antibiotic residue immunoassay. Wherein the anti-GM antibody, namely an anti-gentamicin antibody, is targeted to a gentamicin (GM) antigen epitope, and the anti-GM antibody, namely the anti-gentamicin antibody, is targeted to a gentamicin (GM) antigen epitope; the gene comprises HCDR1, HCDR2 and HCDR3 of which the amino acid sequences are shown as SEQ ID No.1, 2 and 3 and / or LCDR1, LCDR2 and LCDR3 of which the amino acid sequences are shown as SEQ ID No.4, 5 and 6, and is specifically combined with a gentamicin antigen epitope by virtue of the HCDR1, the HCDR2 and the LCDR3. The invention also discloses polynucleotide for coding the gene and a kit / non-diagnostic method related to the polynucleotide, application and the like. Compared with the prior art, the anti-GM antibody can be highly specifically combined with a gentamicin epitope, and no cross reaction exists; the polynucleotides, kits / non-diagnostic methods, applications and the like are also beneficial to rapid and accurate screening of gentamicin residues in a sample.

The invention belongs to the field of microbial technology, and specifically relates to a strain of acid fish lactobacillus PKC6 and an application thereof. The acid fish lactobacillus PKC6 is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration Committee, and the deposit number is CGMCC NO. 27201. The new strain of acid fish lactobacillus PKC6 screened by the invention is resistant to gentamicin, polymyxin B, vancomycin, kanamycin and norfloxacin, sensitive to ampicillin, penicillin, cephalexin and doxycycline, and has a good inhibitory effect on Escherichia coli and Staphylococcus aureus; the strain screened by the invention, after being compounded with a composite enzyme and co-fermented with palm meal, can greatly increase the content of nutrients in the fermentation product, and has a more obvious promoting effect on the intestinal development of raised animals, thereby achieving the purpose of improving the palatability of feed and promoting intestinal development.

Among hospital–acquired infections, Pseudomonas aeruginosa-associated urinary tract infections (UTIs) are mainly caused by indwelling urethral catheters (catheter-associated UTIs or CAUTIs) and are difficult to treat, resulting in high rates of morbidity among hospitalized patients. While antibiotics can successfully treat bacteria in the bladder lumen, they are inefficient at crossing stratified urothelium plasma membranes to kill persistent intracellular bacterial communities (IBCs). Herein, UTI IBCs are targeted by locally delivering the antibiotic gentamicin and / or the steroid hormone estradiol via polymeric nanogels conjugated with a cell-penetrating peptide Cys-Gly-Lys-Arg-Lys (CGKRK - SEQ ID NO:1). This approach delivered more intracellular gentamicin compared to drug delivered in solution in vitro and more estradiol compared to conventional methods. In an acute UTI murine model, the nanogel cell-penetrating peptide drug delivery system facilitated the transport of gentamicin into the urothelium and resulted in > 90% clearance of a uropathogenic P. aeruginosa clinical strain in vivo.

The present application belongs to the technical field of biological bionics, polymer, hydrogel material and the like, and particularly relates to a preparation method of a porous surface fracture internal fixation plate. The gentamicin-chitosan-acetic acid mixed solution is placed in an air compressor, the porous surface of the 3D printed fracture internal fixation plate is sprayed, after completion, the calcium chloride-collagen mixed solution is sprayed, then the fracture internal fixation plate is immersed in sodium hydroxide solution and sodium carbonate solution in sequence, and then freeze-drying is performed, so that the mussel shell bionic porous surface fracture internal fixation plate is obtained. The fracture internal fixation plate obtained by the present application has good biocompatibility, stable performance, mechanical properties close to natural bone and can realize drug loading for local prevention of fracture infection.

The invention discloses antibiotic-loaded in-situ modified platelet-rich fibrin gel as well as a preparation method and application thereof. The antibiotic-loaded in-situ modified platelet-rich fibrin gel is prepared from the following components in percentage by mass: 88 to 92 percent of PRF (platelet-rich fibrin) matrix, 5 to 8 percent of antibiotic and 3 to 4 percent of modifier, the PRF matrix comprises platelets, leukocytes and fibrin, the antibiotic is vancomycin or gentamicin, and the modifier is a zinc ion-containing compound or quaternized chitosan. The gel can be applied to preparation of chronic infection wound dressing, the antibiotic loading rate of the gel is increased, the gel has the slow release characteristic, the accumulative release within 7 days is 82.3 + / -5.6%, the burst release within 24 hours is less than 15%, the wound healing speed is increased by 40%, and the biological membrane clearance rate is 100%.

The invention discloses a fixing material for a medical device implant and a preparation method of the fixing material. The fixing material comprises a degradable polymer base material with a porous structure and a drug sustained-release coating uniformly loaded on the degradable polymer base material, holes in the degradable polymer are uniformly distributed in a staggered array manner, the holes are circular, the aperture of each hole is 0.5-3.0 mm, and the distance between every two adjacent holes is 0.5-3.0 mm; the drug sustained-release coating contains antibiotics; the antibiotics comprise rifampicin, minocycline hydrochloride, vancomycin, tobramycin, gentamicin and the like. The fixing material disclosed by the invention takes the degradable polymer as a base material, and the drug-loading coating containing antibiotics and anti-inflammatory substances is loaded on the surface, so that slow release of antibiotic drugs can be realized, and the fixing material has excellent antibacterial and anti-inflammatory performance and complete absorbability in a biological environment; and the problems of biocompatibility, infection risk and secondary operation removal of the implant for a long time are solved.

The invention discloses D-type mannosylerythritol lipid as well as a preparation method and application thereof. According to the invention, the green and bio-friendly D-type mannosylerythritol lipid is used as a biological bacteriostatic agent and is independently used or combined with other bacteriostatic agents (cefaclor, gentamicin or erythromycin and the like); effective inhibition and even killing of various pathogenic microorganisms (methicillin-resistant staphylococcus aureus, bacillus subtilis or bacillus cereus and the like) are realized. The novel bacteriostatic strategy of the D-type mannosylerythritol lipid provided by the invention not only can effectively realize efficient bacteriostasis, reduce the dosage of an antibacterial agent and reduce the graded bacteriostatic concentration index to 0.09, but also shows good biocompatibility, has a therapeutic index as high as 44.81, and has a wide application prospect.

The invention discloses a multi-component co-pyrolysis biochar material taking mushroom dregs as a main component and a preparation method thereof, and the preparation method comprises the following steps: step 1, selecting gentamicin mushroom dregs as a main component, and wheat straw and kitchen waste as additives as substrates for preparing multi-component co-pyrolysis biochar; 2, optimizing the proportion of the three raw materials by using a mixed material design and a response surface model; step 3, performing pyrolysis at 550 DEG C, and performing oxygen-limited pyrolysis in a muffle furnace for 1 hour; 4, the yield and the specific surface area of the biochar serve as response indexes, and finally the optimal proportion of the three raw materials is obtained through model optimization. According to the prepared biochar material, the specific surface area and the pore structure of the biochar are remarkably improved, and the biochar material shows better application prospects in the fields of pollutant adsorption, soil improvement, environmental remediation and the like; through innovative raw material combination and process route, various key properties of the biochar are remarkably improved, and the biochar has wide market application value and scientific research significance.

The invention discloses a TIL cell culture medium and a TIL cell culture method, an activation and proliferation culture medium of TIL cells comprises the following components: a T cell serum-free culture medium, IL-2, platelet lysate and gentamicin, a CD3 monoclonal antibody, a CD28 monoclonal antibody and phytolectin PHA-P are added for cell activation, and then the activation and proliferation culture medium is used for activation and proliferation culture. Wherein CD3 and CD28 antibodies are used in combination to stimulate TIL cells and simulate the double-signal effect of in-vivo TIL cell activation, meanwhile, PHA-P is utilized, by means of the characteristics of PHA-P, small gonorrhea cells are stimulated in vitro to be converted into gonorrhea cells with immunocompetence, division of the gonorrhea cells is promoted, and the proliferation capacity of the TIL cells is improved through the synergistic effect of PHA-P and IL-2; platelet lysate is used for replacing plasma of a patient and is used as an efficient culture supplement for supporting TIL cell growth; finally, more TIL cells are obtained through the technology, and the number of immune cells required by clinical effective treatment can be sufficiently met.

The invention discloses a method for rapidly detecting intracellular mycobacterium tuberculosis, which comprises the following steps: marking mycobacterium tuberculosis by using mScarlet, marking host cells by CFSE, rinsing part of extracellular bacteria which do not enter macrophages by PBS and sterilizing by gentamicin, detecting the survival amount of the intracellular mycobacterium tuberculosis by using a flow cytometer, and completing sample analysis within several hours. The experimental period is remarkably shortened, the research efficiency is improved, and the method is suitable for large-scale sample analysis.

The invention relates to the technical field of microbiological detection, in particular to a method for detecting clostridium in cosmetics. The invention provides a modified reinforced clostridium culture medium, a modified Columbia agar culture medium and a method for detecting clostridium in cosmetics, and the method is specifically as follows: the modified reinforced clostridium culture medium can inhibit the activity of preservatives in the cosmetics and improve the clostridium detection rate by adding Tween 80, lecithin and sodium thiosulfate; according to the modified Columbia agar culture medium, polymyxin B and gentamicin in a specific proportion are added, so that interference of non-target bacteria can be effectively inhibited, and detection specificity is enhanced. Based on the two modified culture media, the detection method established by the invention adopts a sample preparation mode of normal temperature and heating dual-condition treatment, and the detection rate of target bacteria can be remarkably improved. The method has the advantages of rapidness, high efficiency, high specificity, high detection rate, low cost, simplicity and convenience in operation and the like, and is particularly suitable for rapid screening requirements of supervision departments and detection mechanisms.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus johnsonii with a function of resisting multiple drug-resistant bacterium biological membranes and anti-inflammatory activity and application of the lactobacillus johnsonii. The lactobacillus johnsonii A25041 provided by the invention has strong inhibition and anti-biofilm activity on multi-drug-resistant gram-negative bacteria (including escherichia coli, pseudomonas aeruginosa and acinetobacter baumannii) and methicillin-resistant staphylococcus aureus, and the lactobacillus johnsonii A25041 has good acid-base tolerance and gastrointestinal fluid tolerance, so that the lactobacillus johnsonii A25041 can be used for preparing medicines for preventing and treating various diseases. The composition shows excellent anti-inflammatory activity and oxidation resistance, does not have hemolysis capacity, is good in safety, has natural drug resistance to gentamicin, tetracycline, ciprofloxacin, lincomycin and compound sulfamethoxazole, can be used together with antibiotics at the same time or in sequence, maintains intestinal microecological balance and relieves antibiotic burden while exerting the sterilization effect of the antibiotics, and has a good application prospect. The method can be widely applied to functional food development, and has important application value in the field of biological medicine and daily chemical products.

The invention discloses an IS26-based drug-resistant gene resistance and control plasmid, an engineering bacterium, a fungicide and application, and belongs to the technical field of gene engineering, and the nucleotide sequence of the IS26-based drug-resistant gene resistance and control plasmid is shown as SEQ ID NO: 1 or SEQ ID NO: 2. Engineering bacteria containing the drug-resistant gene resistance and control plasmid and a microbial inoculum can be used for resistance and control of drug-resistant genes, and the insertion sequence IS26 is specifically destroyed, so that the number of the drug-resistant genes is fundamentally reduced, spreading of the drug-resistant genes is blocked, and a precise solution is provided for treatment of drug resistance; the compound has broad-spectrum drug-resistant bacterium resistance and control performance, gentamicin, tetracycline, chloramphenicol and ampicillin drug-resistant bacteria in wastewater can be effectively prevented and controlled, and the highest resistance and control efficiency can reach 83.76%; the plasmid, the engineering bacterium and the fungicide are low in preparation cost, can be used for in-situ wastewater treatment, are low in treatment cost and good in effect, and have wide application prospects.

Disclosed is a novel pharmaceutical composition for treating ear infections in animals in a single dose, wherein the pharmaceutical composition comprises a gentamicin antibiotic, a posaconazole antifungal drug, and a mometasone anti-inflammatory drug.

The invention relates to the technical field of organic chemistry, and particularly discloses an isoxazole amide derivative with a structure shown in a general formula III as well as a preparation method and application of the isoxazole amide derivative. The isoxazole amide derivative disclosed by the invention shows remarkable antibacterial activity on staphylococcus aureus, escherichia coli, methicillin-resistant staphylococcus aureus (MRSA) and fluoroquinolone-resistant escherichia coli (FREC), the activity of part of compounds is superior to that of gentamicin, and the isoxazole amide derivative has broad-spectrum antibacterial potential and can be used for preparing medicines for treating related bacterial infections.

The present invention belongs to the field of antimicrobial peptides, and specifically relates to an antimicrobial peptide, antimicrobial product and application that inhibits a variety of human pathogens. The antimicrobial peptide is named CAMP1229NC, and is obtained by capping the polypeptide shown in SEQ ID NO:1 with an N-terminal acetyl group and a C-terminal amino group. The antimicrobial peptide exhibits significant antibacterial activity against Staphylococcus aureus standard strain ATCC12600, Pseudomonas aeruginosa PAO1, Klebsiella pneumoniae, Acinetobacter baumannii standard strain ATCC19606, methicillin-resistant Staphylococcus aureus ATCC33591, methicillin- and gentamicin-resistant Staphylococcus aureus ATCC33592 and Acinetobacter baumannii bio-53272 resistant to multiple antibiotics, effectively overcoming the limitations of traditional antimicrobial peptides.

The application discloses an anti-GM antibody and application thereof, and relates to the technical field of antibiotic residue immunoassay. The anti-GM antibody, i.e. an anti-gentamicin antibody, targets a gentamicin (GM) antigen epitope; the anti-GM antibody comprises HCDR1, HCDR2, HCDR3 with an amino acid sequence as shown in SEQ ID No. 1, 2, 3 and / or LCDR1, LCDR2, LCDR3 with an amino acid sequence as shown in SEQ ID No. 4, 5, 6, and specifically binds to the gentamicin antigen epitope. The application further discloses polynucleotides encoding the anti-GM antibody and a kit / non-diagnostic method and application related to the anti-GM antibody. Compared with the prior art, the anti-GM antibody can highly specifically bind to the gentamicin antigen epitope without cross-reaction; and the polynucleotides, the kit / non-diagnostic method and the application are also beneficial to rapid and accurate screening of gentamicin residues in samples.

The invention relates to the technical field of assisted reproduction, in particular to a sperm gradient separating medium and a preparation method and application thereof. The sperm gradient separation liquid comprises a gradient liquid A and a gradient liquid B; the gradient solution A and the gradient solution B comprise sodium chloride, potassium chloride, magnesium sulfate, monopotassium phosphate, calcium chloride, sodium pyruvate, glucose, gentamicin, a sodium lactate solution, 4-hydroxyethylpiperazine ethanesulfonic acid, sodium bicarbonate, human serum albumin, progesterone and Percoll PLUS; the Percoll PLUS mass concentration of the gradient solution A is 70 to 85 percent; the Percoll PLUS mass concentration of the gradient solution B is 35 to 45 percent; the mass ratio of sodium chloride to potassium chloride to magnesium sulfate to calcium chloride in the gradient solution A to the gradient solution B is (5-5.5): (0.3-0.38): (0.25-0.30): (0.24-0.28); the mass ratio of the human serum albumin to the progesterone in the gradient solution A to the gradient solution B is (3-7): (0.00075-0.00085). The sperm gradient separation liquid can improve the activity of forward sperms after treatment, the activity of sperms after treatment, the recovery rate of active sperms and the improvement rate of sperm activity.

The invention relates to the field of skin wound repair, and particularly discloses an anti-scar hydrogel for skin wound repair and a preparation method thereof.The preparation method comprises the steps that 1, 10 g of silicon dioxide nanospheres with the average particle size being 50-500 nanometers are selected as a template; step 2, dispersing the template in 1000ml of aqueous solution containing 100-300g of acrylic acid monomer to obtain solution A; 3, 3-9 g of ammonium persulfate is added into the solution A for a polymerization reaction, the reaction temperature ranges from 30 DEG C to 50 DEG C, the pH ranges from 6.5 to 7.5, the reaction time ranges from 2 h to 6 h, and a solution B is obtained after the reaction; active components such as gentamicin, thrombin and epidermal growth factors are uniformly dispersed in the porous hydrogel, the components have a synergistic effect, wound healing is effectively promoted, scar formation is inhibited, gentamicin has an antibacterial effect, and the risk of wound infection can be reduced; thrombin can accelerate blood coagulation and reduce bleeding; and the epidermal growth factor can promote the growth of epithelial cells and accelerate wound healing.

The invention provides nitrate reducing bacteria C381 as well as a method and application thereof. The nitrate reducing bacteria C381 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36842; the invention provides a novel oral cavity probiotic nitrate reducing bacteria C381, the nitrate reducing bacteria C381 has an inhibition effect on periodontal disease bacteria, and the nitrate reducing bacteria C381 is sensitive to antibiotics such as erythromycin, piperacillin, minocycline, doxycycline, cefazolin, tetracycline, amikacin, gentamicin, vancomycin and cefoperazone, and can be used for preparing oral cavity probiotics for treating periodontal disease bacteria. And the product has good survival ability in the oral cavity.

The present invention belongs to the field of antimicrobial peptides, and specifically relates to an antimicrobial peptide, antimicrobial product and application with a broad-spectrum antimicrobial effect. The antimicrobial peptide is named CAMP502NC, and is obtained by capping the polypeptide shown in SEQ ID NO:1 with an N-terminal acetyl group and capping the C-terminal amino group. The antimicrobial peptide exhibits significant antimicrobial activity against Staphylococcus aureus standard strain ATCC12600, Pseudomonas aeruginosa PAO1, Klebsiella pneumoniae, Acinetobacter baumannii standard strain ATCC19606, methicillin-resistant Staphylococcus aureus ATCC33591, methicillin- and gentamicin-resistant Staphylococcus aureus ATCC33592 and Acinetobacter baumannii bio-53272 resistant to multiple antibiotics, effectively overcoming the limitations of traditional antimicrobial peptides.

The invention relates to the technical field of cell differentiation, in particular to a method for induced differentiation of menstrual blood-derived endometrial mesenchymal stem cells into chondrocytes, which comprises an endometrial tissue preservation solution and a novel induction reagent, the endometrial tissue preserving fluid is prepared from 500mL of DMEM / F-12 basal culture medium, penicillin, streptomycin and gentamicin; the novel induction reagent comprises a basic induction reagent, hydroxyapatite, patient serum and articular cavity tissue fluid. In some existing induced differentiation methods, the number, activity and purity of cells cannot meet the requirements of clinical application, compared with the prior art, a novel induction reagent is used, some substances are added on the basis of a basic induction reagent, and an endometrial tissue preservation solution is prepared to prevent bacteria from influencing the cells, so that the method has the advantages of simple operation and low cost. And the number, activity and purity of cartilage cells differentiated by induction are improved, so that the method can meet the requirements of clinical application.

The utility model relates to the technical field of medical rivets, and provides an antibacterial absorbable gentamicin-loaded silk fibroin medical rivet and a mold. The rivet rod of the rivet is provided with an inner hexagonal notch and a wire passing through hole, and the screw pitches of threads at the near end and the far end of the rivet are different, so that the fixing strength of the rivet in sclerotin can be enhanced; the material overcomes the defects that metal cannot be absorbed and polylactic acid is used as a material, and can be degraded in time, resist bacteria, reduce foreign body reaction and inflammation and promote tissue healing; and the internal structural design of the rivet can meet the use requirement of the rivet under an arthroscopic surgery. The personalized mold is suitable for the special preparation process of fibroin, a mold body is composed of an upper mold body and a lower mold body which are detachable, and a pouring cavity formed by butt joint of pouring grooves of the upper mold body and the lower mold body can precisely mold the appearance of a rivet; the inner core assembly comprises an inner hexagonal column, a circular column and a positioning seat, and the inner structure of the rivet can be accurately formed. And the rivet is integrally formed to avoid later processing, so that high standard and consistency of products are ensured. The mold is ingenious in overall matching, convenient to assemble, maintain and operate, and time-saving and labor-saving.