17 results about "Gentamicin" patented technology

The application discloses application of antibacterial peptide BMAP-28 and antibiotics in combined preparation of antibacterial drugs and belongs to the technical field of biological medicine. The antibiotics are selected from one or more of the following: ciprofloxacin, gentamicin and cefixime. The application combines the antibacterial peptide BMAP-28 with antibiotics such as ciprofloxacin and gentamicin, finds that the combination has a significant synergistic antibacterial effect on Enterobacter sakazii, can greatly save the dosage of the drug, reduce the toxic side effect and block the evolution process of drug resistance. Through the destruction of the bacterial cell membrane and the assistance of the antibiotic effect, the combination can effectively overcome the multiple drug resistance, widen the antibacterial spectrum and has a wide clinical application prospect.

The invention discloses a fixing material for a medical device implant and a preparation method of the fixing material. The fixing material comprises a degradable polymer base material with a porous structure and a drug sustained-release coating uniformly loaded on the degradable polymer base material, holes in the degradable polymer are uniformly distributed in a staggered array manner, the holes are circular, the aperture of each hole is 0.5-3.0 mm, and the distance between every two adjacent holes is 0.5-3.0 mm; the drug sustained-release coating contains antibiotics; the antibiotics comprise rifampicin, minocycline hydrochloride, vancomycin, tobramycin, gentamicin and the like. The fixing material disclosed by the invention takes the degradable polymer as a base material, and the drug-loading coating containing antibiotics and anti-inflammatory substances is loaded on the surface, so that slow release of antibiotic drugs can be realized, and the fixing material has excellent antibacterial and anti-inflammatory performance and complete absorbability in a biological environment; and the problems of biocompatibility, infection risk and secondary operation removal of the implant for a long time are solved.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus johnsonii with a function of resisting multiple drug-resistant bacterium biological membranes and anti-inflammatory activity and application of the lactobacillus johnsonii. The lactobacillus johnsonii A25041 provided by the invention has strong inhibition and anti-biofilm activity on multi-drug-resistant gram-negative bacteria (including escherichia coli, pseudomonas aeruginosa and acinetobacter baumannii) and methicillin-resistant staphylococcus aureus, and the lactobacillus johnsonii A25041 has good acid-base tolerance and gastrointestinal fluid tolerance, so that the lactobacillus johnsonii A25041 can be used for preparing medicines for preventing and treating various diseases. The composition shows excellent anti-inflammatory activity and oxidation resistance, does not have hemolysis capacity, is good in safety, has natural drug resistance to gentamicin, tetracycline, ciprofloxacin, lincomycin and compound sulfamethoxazole, can be used together with antibiotics at the same time or in sequence, maintains intestinal microecological balance and relieves antibiotic burden while exerting the sterilization effect of the antibiotics, and has a good application prospect. The method can be widely applied to functional food development, and has important application value in the field of biological medicine and daily chemical products.

The invention provides nitrate reducing bacteria C381 as well as a method and application thereof. The nitrate reducing bacteria C381 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36842; the invention provides a novel oral cavity probiotic nitrate reducing bacteria C381, the nitrate reducing bacteria C381 has an inhibition effect on periodontal disease bacteria, and the nitrate reducing bacteria C381 is sensitive to antibiotics such as erythromycin, piperacillin, minocycline, doxycycline, cefazolin, tetracycline, amikacin, gentamicin, vancomycin and cefoperazone, and can be used for preparing oral cavity probiotics for treating periodontal disease bacteria. And the product has good survival ability in the oral cavity.

The utility model relates to the technical field of medical rivets, and provides an antibacterial absorbable gentamicin-loaded silk fibroin medical rivet and a mold. The rivet rod of the rivet is provided with an inner hexagonal notch and a wire passing through hole, and the screw pitches of threads at the near end and the far end of the rivet are different, so that the fixing strength of the rivet in sclerotin can be enhanced; the material overcomes the defects that metal cannot be absorbed and polylactic acid is used as a material, and can be degraded in time, resist bacteria, reduce foreign body reaction and inflammation and promote tissue healing; and the internal structural design of the rivet can meet the use requirement of the rivet under an arthroscopic surgery. The personalized mold is suitable for the special preparation process of fibroin, a mold body is composed of an upper mold body and a lower mold body which are detachable, and a pouring cavity formed by butt joint of pouring grooves of the upper mold body and the lower mold body can precisely mold the appearance of a rivet; the inner core assembly comprises an inner hexagonal column, a circular column and a positioning seat, and the inner structure of the rivet can be accurately formed. And the rivet is integrally formed to avoid later processing, so that high standard and consistency of products are ensured. The mold is ingenious in overall matching, convenient to assemble, maintain and operate, and time-saving and labor-saving.

The invention provides bacillus C367 as well as a method and application thereof. The bacillus C367 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36841; the bacillus C367 is separated from the Inner Mongolia cheese; the bacillus C367 can be used for preparing medicines for treating or preventing periodontal diseases; the invention provides a novel oral cavity probiotic bacillus C367, the bacillus C367 has an inhibition effect on periodontal disease bacteria, and the bacillus C367 is sensitive to antibiotics such as minocycline, amikacin, tetracycline, erythromycin, cefoperazone, streptomycin, kanamycin, gentamicin, vancomycin and ceftriaxone, and has good survival ability in an oral cavity.

N-Functionalized Indole Compounds as Antimicrobial Agents and Process for Preparation Thereof The present invention relates to novel N-functionalized indole compounds and their synthesis using ionic liquids as green reagents and / or solvents in a two to four-step process. The compounds exhibit potent antibacterial activity against multi-drug resistant and susceptible gram-positive bacteria, including Staphylococcus aureus and Enterococcus sp. Synergistic effects are observed when combined with gentamicin or levofloxacin, including activity against resistant MRSA (NRS 119). In-vivo efficacy is demonstrated in a murine skin infection model via topical and oral administration.

The invention discloses application of BSA (Bovine Serum Albumin) in improving the performance of low-density boar semen, and the BSA is particularly suitable for 0.5 billion / 60ml low-density boar semen. Accordingly, the inventor also develops a boar semen diluent, which comprises a basic solution, a nutritional agent, a buffer agent and an electrolyte, and also comprises a core film protecting agent, an antioxidant and a composite antibacterial agent, the core film protecting agent is BSA, the nutritional agent is composed of glucose and fructose, the antioxidant is composed of glutathione and arbutin, and the composite antibacterial agent is composed of penicillin, streptomycin and gentamicin. By accurately optimizing the BSA addition concentration and cooperating with glucose and fructose (dual-energy supply), a high-quality sperm protection microenvironment system of'targeted film protection-environment stabilization-energy supply 'is constructed, the problems of short preservation time and poor sperm form integrity when low-density sperms are treated by conventional diluents are solved, and cost reduction and efficiency improvement of large-scale pig farms can be realized.

The invention belongs to the technical field of cell culture, and discloses a bone marrow cell culture medium and a preparation method thereof, and a method for in-vitro culture of bone marrow cells, the bone marrow cell culture medium is composed of a basic culture medium, streptomycin, gentamicin, a platelet lysis buffer, and glutamate enzyme; the final concentration of streptomycin is 50-100 [mu] g / mL, the final concentration of gentamicin is 20-50 [mu] g / mL, the final concentration of platelet lysate is 5-15% (v / v), and the final concentration of glutamate enzyme is 0.1-1 mmol / L. The bone marrow cell culture medium can efficiently support in-vitro proliferation of bone marrow cells, through specific combination and appropriate concentration control of the culture medium, the medium-term cell acquisition amount can be remarkably increased, and medium-term cell chromosomes are complete in morphology and good in dispersity; the standard requirements of clinical bone marrow cell chromosome karyotype analysis, blood disease diagnosis and cell genetics experiments in the scientific research field can be met.

The application discloses a bovine respiratory pathogen preservation solution and a preparation method and application thereof, and belongs to the technical field of biological body preservation reagents. The bovine respiratory pathogen preservation solution provided by the application comprises appropriate amounts of carboxymethyl cellulose sodium, gentamicin, fetal bovine serum, trypsin, phosphate and sodium chloride and the like. The bovine respiratory pathogen preservation solution can effectively protect the integrity of the bovine respiratory pathogen under normal temperature conditions, can prevent bacterial contamination, is beneficial to subsequent bovine respiratory pathogen separation, culture and identification, and thus the distribution and infection of the bovine respiratory pathogen can be correctly detected, and then targeted prevention and treatment measures can be taken.

This invention discloses a combination of Western and traditional Chinese medicine for treating gastric ulcers. The combination comprises ① gentamicin (80,000 units / vial) and a combination of gentamicin and a remedy called "Weidele". This combination has anti-inflammatory, laxative, and anti-inflammatory effects when taken orally. It is effective in treating inflammation and is safe to use with an extremely low recurrence rate. Improvement is seen within fifteen days, with no obvious toxic side effects. After approximately ten days of symptomatic treatment with the combination of gentamicin (80,000 units / vial) and remedyle, the condition improved, symptoms disappeared, and a follow-up gastroscopy showed improved lesion absorption. Continued anti-inflammatory treatment and a follow-up gastroscopy at three months were then performed.

The application discloses a doramectin monoclonal antibody, a detection test strip and application thereof, and relates to the field of animal health. 50 The heavy chain and light chain variable regions of the monoclonal antibody have specific complementarity determining region sequences, and the half inhibition concentration IC of the monoclonal antibody to doramectin is as low as 3-5 ng / mL, which shows excellent sensitivity; the cross reaction rates of the monoclonal antibody to abamectin, ivermectin and acetyl abamectin are 75%, 75% and 60% respectively, and the monoclonal antibody has no cross reaction with common antibiotics such as tetracycline and gentamicin. Based on the antibody, the application also develops a colloidal gold immunochromatography test strip, which is simple in operation, rapid in detection, visual in result, and has a detection limit of 5 ng / mL, and is suitable for on-site rapid screening of doramectin and abamectin drug residues in animal-derived food, and has a good application prospect.

Provided are a tetragenococcus halophilus with an inhibitory effect on bacillus cereus and a use thereof. The preservation number of the tetragenococcus halophilus is GDMCC 64945. The tetragenococcus halophilus is isolated from fermented bean curd, and colonies of the tetragenococcus halophilus are milky white, small and round on a solid medium; The tetragenococcus halophilus is Gram-positive, and when observed under a microscope, the morphology thereof is mostly diplococci or tetrads, with no chain-like formations. The strain is sensitive to eight antibiotics such as ampicillin, gentamicin and kanamycin, and has no drug resistance gene and virulence gene. A bacterial suspension of the strain has a good inhibitory effect on a bacillus cereus strain isolated from the fermented bean curd, and when the strain is applied to a fermented bean curd fermentation system, the growth of bacillus cereus in the system can be effectively inhibited, and the content of enterotoxin in the system is reduced.

The invention discloses a Rongchang pig source ZH30 strain and application thereof, and belongs to the technical field of agricultural livestock feed additives. The ZH30 strain provided by the invention has bacteriostatic activity on a plurality of common pig pathogenic bacteria such as escherichia coli, staphylococcus aureus, salmonella typhimurium, salmonella pullorum and enterococcus faecalis, is wide in antibacterial spectrum, and is beneficial to reducing the load of pathogenic bacteria in intestinal tracts of pigs. Meanwhile, the ZH30 strain is sensitive to streptomycin, gentamicin and ampicillin, infectious microbe pollution can be conveniently controlled through a conventional antibiotic means in the fermentation and preparation production process, and meanwhile the drug resistance spreading risk is reduced. As a functional strain of a probiotic preparation for pigs, the ZH30 strain disclosed by the invention can improve the intestinal health of pigs and improve the mechanical barrier integrity of the intestinal tracts, has potential application values of reducing the diarrhea risk of piglets and improving the feed utilization rate and the production performance, and is of great significance to the development of animal husbandry and feed industry.

The present invention relates to a pharmaceutical composition for the prevention or treatment of hearing loss containing ammonium lactate as an active ingredient. It has been confirmed that treating hair cells damaged by gentamicin with ammonium lactate increases the number of normal hair cells, and therefore, a pharmaceutical composition containing ammonium lactate as an active ingredient can be used to prevent or treat hearing loss, a side effect of using the antibiotic ammonium lactate.

This invention, entitled "A Fertilization Culture Medium, Its Preparation Method, and Its Application," belongs to the field of assisted reproductive technology. The technical problem it aims to solve is that existing technologies, due to the generation of sperm DNA fragments during the culture process, lead to a decrease in overall sperm quality, limiting fertilization rate and blastocyst formation rate. This invention proposes a fertilization culture medium that can improve overall sperm quality and thus increase fertilization rate. The key technical points are: a fertilization culture medium comprising the following components: sodium chloride, potassium chloride, alkali metal dihydrogen phosphate, calcium chloride, magnesium sulfate, sodium bicarbonate, sodium lactate, sodium pyruvate, glucose, fructose, disodium edetate, gentamicin, human serum albumin, alanylglutamine, taurine, non-essential amino acids, and testosterone.

The invention discloses a method for producing gentamicin B through fermentation of micromonas aculeata. The method adopts a substrate feedback strategy for continuous feeding, and the feeding opportunity and the feeding speed are determined by detecting the glucose content of a substrate in the fermentation process. And animal components such as fish meal and the like are not contained, so that the titer stability of different batches is improved, and the bacterial contamination probability is also reduced. One or more of potassium sulfate, sodium chloride, dipotassium phosphate and monopotassium phosphate are used for replacing nitrate which is an easy-to-explode compound in the fermentation culture medium, so that the purchasing difficulty is solved, and the normal operation of large-scale production is ensured. And the fermentation period is shortened from the original 140 to 168 hours to 114 to 120 hours. Compared with the traditional process, the fermentation titer of gentamicin B is improved by 61.02%-66.63%, and the production cost is greatly reduced.