31 results about "Gentamicin" patented technology

The invention belongs to the technical field of biology, and particularly relates to bifidobacterium longum subsp.infantis FMBL B250020GXY for metabolizing breast milk oligosaccharide and application of the bifidobacterium longum subsp.infantis FMBL B250020GXY, the bifidobacterium longum subsp.infantis FMBL B250020GXY is preserved in China Center for Type Culture Collection on September 1, 2025, and the preservation number is CCTCC M 20251931; three kinds of breast milk oligosaccharides, namely 2 '-fucosyllactose (2'-FL), lactose-N-neotetraose (LNnT) and lactose-N-tetraose (LNT), can be metabolized; plant source carbohydrates such as fructo-oligosaccharide and synanthrin can be metabolized; the compound has a relatively strong capability of removing DPPH and ABTS free radicals; a good inhibition effect is achieved on common pathogenic bacteria; good tolerance to acid and cholate is achieved; the strain is sensitive to gentamicin, streptomycin, tetracycline, chloramphenicol and vancomycin; the compound can be used for preparing antioxidant products, medicines for inhibiting pathogenic bacteria, infant foods and fermented foods, and has a wide application prospect.

The application discloses application of antibacterial peptide BMAP-28 and antibiotics in combined preparation of antibacterial drugs and belongs to the technical field of biological medicine. The antibiotics are selected from one or more of the following: ciprofloxacin, gentamicin and cefixime. The application combines the antibacterial peptide BMAP-28 with antibiotics such as ciprofloxacin and gentamicin, finds that the combination has a significant synergistic antibacterial effect on Enterobacter sakazii, can greatly save the dosage of the drug, reduce the toxic side effect and block the evolution process of drug resistance. Through the destruction of the bacterial cell membrane and the assistance of the antibiotic effect, the combination can effectively overcome the multiple drug resistance, widen the antibacterial spectrum and has a wide clinical application prospect.

The invention discloses an anti-GM antibody and application thereof, and relates to the technical field of antibiotic residue immunoassay. Wherein the anti-GM antibody, namely an anti-gentamicin antibody, is targeted to a gentamicin (GM) antigen epitope, and the anti-GM antibody, namely the anti-gentamicin antibody, is targeted to a gentamicin (GM) antigen epitope; the gene comprises HCDR1, HCDR2 and HCDR3 of which the amino acid sequences are shown as SEQ ID No.1, 2 and 3 and / or LCDR1, LCDR2 and LCDR3 of which the amino acid sequences are shown as SEQ ID No.4, 5 and 6, and is specifically combined with a gentamicin antigen epitope by virtue of the HCDR1, the HCDR2 and the LCDR3. The invention also discloses polynucleotide for coding the gene and a kit / non-diagnostic method related to the polynucleotide, application and the like. Compared with the prior art, the anti-GM antibody can be highly specifically combined with a gentamicin epitope, and no cross reaction exists; the polynucleotides, kits / non-diagnostic methods, applications and the like are also beneficial to rapid and accurate screening of gentamicin residues in a sample.

Among hospital–acquired infections, Pseudomonas aeruginosa-associated urinary tract infections (UTIs) are mainly caused by indwelling urethral catheters (catheter-associated UTIs or CAUTIs) and are difficult to treat, resulting in high rates of morbidity among hospitalized patients. While antibiotics can successfully treat bacteria in the bladder lumen, they are inefficient at crossing stratified urothelium plasma membranes to kill persistent intracellular bacterial communities (IBCs). Herein, UTI IBCs are targeted by locally delivering the antibiotic gentamicin and / or the steroid hormone estradiol via polymeric nanogels conjugated with a cell-penetrating peptide Cys-Gly-Lys-Arg-Lys (CGKRK - SEQ ID NO:1). This approach delivered more intracellular gentamicin compared to drug delivered in solution in vitro and more estradiol compared to conventional methods. In an acute UTI murine model, the nanogel cell-penetrating peptide drug delivery system facilitated the transport of gentamicin into the urothelium and resulted in > 90% clearance of a uropathogenic P. aeruginosa clinical strain in vivo.

The present application belongs to the technical field of biological bionics, polymer, hydrogel material and the like, and particularly relates to a preparation method of a porous surface fracture internal fixation plate. The gentamicin-chitosan-acetic acid mixed solution is placed in an air compressor, the porous surface of the 3D printed fracture internal fixation plate is sprayed, after completion, the calcium chloride-collagen mixed solution is sprayed, then the fracture internal fixation plate is immersed in sodium hydroxide solution and sodium carbonate solution in sequence, and then freeze-drying is performed, so that the mussel shell bionic porous surface fracture internal fixation plate is obtained. The fracture internal fixation plate obtained by the present application has good biocompatibility, stable performance, mechanical properties close to natural bone and can realize drug loading for local prevention of fracture infection.

The invention discloses antibiotic-loaded in-situ modified platelet-rich fibrin gel as well as a preparation method and application thereof. The antibiotic-loaded in-situ modified platelet-rich fibrin gel is prepared from the following components in percentage by mass: 88 to 92 percent of PRF (platelet-rich fibrin) matrix, 5 to 8 percent of antibiotic and 3 to 4 percent of modifier, the PRF matrix comprises platelets, leukocytes and fibrin, the antibiotic is vancomycin or gentamicin, and the modifier is a zinc ion-containing compound or quaternized chitosan. The gel can be applied to preparation of chronic infection wound dressing, the antibiotic loading rate of the gel is increased, the gel has the slow release characteristic, the accumulative release within 7 days is 82.3 + / -5.6%, the burst release within 24 hours is less than 15%, the wound healing speed is increased by 40%, and the biological membrane clearance rate is 100%.

The invention discloses a fixing material for a medical device implant and a preparation method of the fixing material. The fixing material comprises a degradable polymer base material with a porous structure and a drug sustained-release coating uniformly loaded on the degradable polymer base material, holes in the degradable polymer are uniformly distributed in a staggered array manner, the holes are circular, the aperture of each hole is 0.5-3.0 mm, and the distance between every two adjacent holes is 0.5-3.0 mm; the drug sustained-release coating contains antibiotics; the antibiotics comprise rifampicin, minocycline hydrochloride, vancomycin, tobramycin, gentamicin and the like. The fixing material disclosed by the invention takes the degradable polymer as a base material, and the drug-loading coating containing antibiotics and anti-inflammatory substances is loaded on the surface, so that slow release of antibiotic drugs can be realized, and the fixing material has excellent antibacterial and anti-inflammatory performance and complete absorbability in a biological environment; and the problems of biocompatibility, infection risk and secondary operation removal of the implant for a long time are solved.

The invention relates to the technical field of microbiological detection, in particular to a method for detecting clostridium in cosmetics. The invention provides a modified reinforced clostridium culture medium, a modified Columbia agar culture medium and a method for detecting clostridium in cosmetics, and the method is specifically as follows: the modified reinforced clostridium culture medium can inhibit the activity of preservatives in the cosmetics and improve the clostridium detection rate by adding Tween 80, lecithin and sodium thiosulfate; according to the modified Columbia agar culture medium, polymyxin B and gentamicin in a specific proportion are added, so that interference of non-target bacteria can be effectively inhibited, and detection specificity is enhanced. Based on the two modified culture media, the detection method established by the invention adopts a sample preparation mode of normal temperature and heating dual-condition treatment, and the detection rate of target bacteria can be remarkably improved. The method has the advantages of rapidness, high efficiency, high specificity, high detection rate, low cost, simplicity and convenience in operation and the like, and is particularly suitable for rapid screening requirements of supervision departments and detection mechanisms.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus johnsonii with a function of resisting multiple drug-resistant bacterium biological membranes and anti-inflammatory activity and application of the lactobacillus johnsonii. The lactobacillus johnsonii A25041 provided by the invention has strong inhibition and anti-biofilm activity on multi-drug-resistant gram-negative bacteria (including escherichia coli, pseudomonas aeruginosa and acinetobacter baumannii) and methicillin-resistant staphylococcus aureus, and the lactobacillus johnsonii A25041 has good acid-base tolerance and gastrointestinal fluid tolerance, so that the lactobacillus johnsonii A25041 can be used for preparing medicines for preventing and treating various diseases. The composition shows excellent anti-inflammatory activity and oxidation resistance, does not have hemolysis capacity, is good in safety, has natural drug resistance to gentamicin, tetracycline, ciprofloxacin, lincomycin and compound sulfamethoxazole, can be used together with antibiotics at the same time or in sequence, maintains intestinal microecological balance and relieves antibiotic burden while exerting the sterilization effect of the antibiotics, and has a good application prospect. The method can be widely applied to functional food development, and has important application value in the field of biological medicine and daily chemical products.

The invention relates to the technical field of organic chemistry, and particularly discloses an isoxazole amide derivative with a structure shown in a general formula III as well as a preparation method and application of the isoxazole amide derivative. The isoxazole amide derivative disclosed by the invention shows remarkable antibacterial activity on staphylococcus aureus, escherichia coli, methicillin-resistant staphylococcus aureus (MRSA) and fluoroquinolone-resistant escherichia coli (FREC), the activity of part of compounds is superior to that of gentamicin, and the isoxazole amide derivative has broad-spectrum antibacterial potential and can be used for preparing medicines for treating related bacterial infections.

The application discloses an anti-GM antibody and application thereof, and relates to the technical field of antibiotic residue immunoassay. The anti-GM antibody, i.e. an anti-gentamicin antibody, targets a gentamicin (GM) antigen epitope; the anti-GM antibody comprises HCDR1, HCDR2, HCDR3 with an amino acid sequence as shown in SEQ ID No. 1, 2, 3 and / or LCDR1, LCDR2, LCDR3 with an amino acid sequence as shown in SEQ ID No. 4, 5, 6, and specifically binds to the gentamicin antigen epitope. The application further discloses polynucleotides encoding the anti-GM antibody and a kit / non-diagnostic method and application related to the anti-GM antibody. Compared with the prior art, the anti-GM antibody can highly specifically bind to the gentamicin antigen epitope without cross-reaction; and the polynucleotides, the kit / non-diagnostic method and the application are also beneficial to rapid and accurate screening of gentamicin residues in samples.

The invention relates to the technical field of assisted reproduction, in particular to a sperm gradient separating medium and a preparation method and application thereof. The sperm gradient separation liquid comprises a gradient liquid A and a gradient liquid B; the gradient solution A and the gradient solution B comprise sodium chloride, potassium chloride, magnesium sulfate, monopotassium phosphate, calcium chloride, sodium pyruvate, glucose, gentamicin, a sodium lactate solution, 4-hydroxyethylpiperazine ethanesulfonic acid, sodium bicarbonate, human serum albumin, progesterone and Percoll PLUS; the Percoll PLUS mass concentration of the gradient solution A is 70 to 85 percent; the Percoll PLUS mass concentration of the gradient solution B is 35 to 45 percent; the mass ratio of sodium chloride to potassium chloride to magnesium sulfate to calcium chloride in the gradient solution A to the gradient solution B is (5-5.5): (0.3-0.38): (0.25-0.30): (0.24-0.28); the mass ratio of the human serum albumin to the progesterone in the gradient solution A to the gradient solution B is (3-7): (0.00075-0.00085). The sperm gradient separation liquid can improve the activity of forward sperms after treatment, the activity of sperms after treatment, the recovery rate of active sperms and the improvement rate of sperm activity.

The invention provides nitrate reducing bacteria C381 as well as a method and application thereof. The nitrate reducing bacteria C381 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36842; the invention provides a novel oral cavity probiotic nitrate reducing bacteria C381, the nitrate reducing bacteria C381 has an inhibition effect on periodontal disease bacteria, and the nitrate reducing bacteria C381 is sensitive to antibiotics such as erythromycin, piperacillin, minocycline, doxycycline, cefazolin, tetracycline, amikacin, gentamicin, vancomycin and cefoperazone, and can be used for preparing oral cavity probiotics for treating periodontal disease bacteria. And the product has good survival ability in the oral cavity.

The utility model relates to the technical field of medical rivets, and provides an antibacterial absorbable gentamicin-loaded silk fibroin medical rivet and a mold. The rivet rod of the rivet is provided with an inner hexagonal notch and a wire passing through hole, and the screw pitches of threads at the near end and the far end of the rivet are different, so that the fixing strength of the rivet in sclerotin can be enhanced; the material overcomes the defects that metal cannot be absorbed and polylactic acid is used as a material, and can be degraded in time, resist bacteria, reduce foreign body reaction and inflammation and promote tissue healing; and the internal structural design of the rivet can meet the use requirement of the rivet under an arthroscopic surgery. The personalized mold is suitable for the special preparation process of fibroin, a mold body is composed of an upper mold body and a lower mold body which are detachable, and a pouring cavity formed by butt joint of pouring grooves of the upper mold body and the lower mold body can precisely mold the appearance of a rivet; the inner core assembly comprises an inner hexagonal column, a circular column and a positioning seat, and the inner structure of the rivet can be accurately formed. And the rivet is integrally formed to avoid later processing, so that high standard and consistency of products are ensured. The mold is ingenious in overall matching, convenient to assemble, maintain and operate, and time-saving and labor-saving.

The invention provides bacillus C367 as well as a method and application thereof. The bacillus C367 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36841; the bacillus C367 is separated from the Inner Mongolia cheese; the bacillus C367 can be used for preparing medicines for treating or preventing periodontal diseases; the invention provides a novel oral cavity probiotic bacillus C367, the bacillus C367 has an inhibition effect on periodontal disease bacteria, and the bacillus C367 is sensitive to antibiotics such as minocycline, amikacin, tetracycline, erythromycin, cefoperazone, streptomycin, kanamycin, gentamicin, vancomycin and ceftriaxone, and has good survival ability in an oral cavity.

The invention discloses tetragenococcus halophilus and an application thereof. The preservation number of the tetragenococcus halophilus is GDMCC (China General Microbiological Culture Collection Center) 66036, and the preservation number of the tetragenococcus halophilus is CGMCC 66036. The tetracoccus halophilus is sensitive to eight antibiotics such as ampicillin, gentamicin and kanamycin, does not have drug-resistant genes and virulence genes, and can generate functional active substances with higher levels, such as protocatechuic acid, hydroxyphenyllactic acid, phenyllactic acid, 4-(methylthio)-2-butanol and 3-indoleacrylic acid. The tetracoccus halophilus has high capacity of generating volatile flavor substances and can be used for improving flavor characteristics of the fermented soybean paste, and the fermented soybean paste prepared by fermentation of the tetracoccus halophilus has rich ester fragrance, high sweet and delicious taste and low bitter aftertaste. The fermented soybean paste prepared by fermentation of the tetragenococcus halophilus has a relatively high level of free amino acid content, especially sweet and delicious free amino acids, and also has a relatively high level of succinic acid and malic acid content.

The invention belongs to the technical field of stem cell culture, and particularly relates to a preparation method of umbilical cord mesenchymal stem cells. Comprising the following steps: placing a collected umbilical cord in a collection solution, preserving at 2-8 DEG C for 66-78h, then taking out the umbilical cord, cutting the umbilical cord into blocks, inoculating the obtained tissue blocks into a T75 cell culture bottle, inversely incubating the tissue blocks, then adding a culture medium for culturing, and carrying out passage when the cell fusion degree reaches 90%. The collecting liquid is prepared from 6 g / L to 10 g / L of sodium chloride, 0.3 g / L to 0.5 g / L of potassium chloride, 0.5 g / L to 2 g / L of glucose, 0.02 g / L to 0.03 g / L of gentamicin, 0.5 g / L to 2 g / L of human serum albumin, 0.02 g / L to 0.03 g / L of transferrin and the balance water. The umbilical cord is preserved by adopting the self-made collection liquid, so that the preservation time of the umbilical cord is prolonged, and the activity of the umbilical cord is enhanced. Meanwhile, according to the preparation method, the separation process of the MSC is optimized, and the separation efficiency and the number of obtained cells are improved.

N-Functionalized Indole Compounds as Antimicrobial Agents and Process for Preparation Thereof The present invention relates to novel N-functionalized indole compounds and their synthesis using ionic liquids as green reagents and / or solvents in a two to four-step process. The compounds exhibit potent antibacterial activity against multi-drug resistant and susceptible gram-positive bacteria, including Staphylococcus aureus and Enterococcus sp. Synergistic effects are observed when combined with gentamicin or levofloxacin, including activity against resistant MRSA (NRS 119). In-vivo efficacy is demonstrated in a murine skin infection model via topical and oral administration.

An antimicrobial composition comprising an antimicrobial compound and a population of metal nanoparticles is provided. The antimicrobial compound is preferably an antibiotic. The metal nanoparticle may comprise silver and / or copper and may also comprise a polymer coating such as poly(N-vinylpyrrolidone). The antimicrobial composition wherein the population of silver nanoparticles is formed by (a) mixing a first aqueous alkaline solution with an aqueous polymer solution to form an aqueous polymer solution; and (b) mixing the aqueous alkaline polymer solution with an aqueous solution of a metal salt to form a solution of polymer-coated metal nanoparticles is provided. A coating or treatment for a surface or substrate comprising the antimicrobial composition is provided. A medical device comprising the coating or treatment is provided. The medical device may be a wound care dressing or a bandage. A silver nanoparticle coated with poly(N-vinylpyrrolidone) is exemplified. Combinations of the PVP-coated silver nanoparticles (AgNP) and various antibiotics were tested. Compositions comprising silver nanoparticles and aminoglycosides, such as kanamycin and gentamicin, were particularly effective. A combination of silver nanoparticles and ebselen was also particularly effective against E. coli and S. aureus.

The invention discloses application of BSA (Bovine Serum Albumin) in improving the performance of low-density boar semen, and the BSA is particularly suitable for 0.5 billion / 60ml low-density boar semen. Accordingly, the inventor also develops a boar semen diluent, which comprises a basic solution, a nutritional agent, a buffer agent and an electrolyte, and also comprises a core film protecting agent, an antioxidant and a composite antibacterial agent, the core film protecting agent is BSA, the nutritional agent is composed of glucose and fructose, the antioxidant is composed of glutathione and arbutin, and the composite antibacterial agent is composed of penicillin, streptomycin and gentamicin. By accurately optimizing the BSA addition concentration and cooperating with glucose and fructose (dual-energy supply), a high-quality sperm protection microenvironment system of'targeted film protection-environment stabilization-energy supply 'is constructed, the problems of short preservation time and poor sperm form integrity when low-density sperms are treated by conventional diluents are solved, and cost reduction and efficiency improvement of large-scale pig farms can be realized.

The invention discloses a tri-modal antibacterial hydrogel dressing as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The three-mode antibacterial hydrogel dressing disclosed by the invention is a composite PVA / QCS / TZG hydrogel dressing; the composite PVA / QCS / TZG hydrogel dressing is prepared by a one-pot method. In the composite PVA / QCS / TZG hydrogel dressing, the mass fraction of the QCSd is 5.25 wt% to 7.25 wt%, and the mass fraction of the TZG NPs is 6.25 wt% to 31.25 wt%. The composite PVA / QCS / TZG hydrogel dressing is prepared through a simple one-pot method, the production method is simple, no cross-linking agent with toxic hidden danger is needed, and the environmental friendliness is high; according to the hydrogel dressing prepared by the invention, a triple sterilization mode consisting of QCS contact killing, Zn < 2 + > destroying and gentamicin eradication is established.

The invention belongs to the technical field of cell culture, and discloses a bone marrow cell culture medium and a preparation method thereof, and a method for in-vitro culture of bone marrow cells, the bone marrow cell culture medium is composed of a basic culture medium, streptomycin, gentamicin, a platelet lysis buffer, and glutamate enzyme; the final concentration of streptomycin is 50-100 [mu] g / mL, the final concentration of gentamicin is 20-50 [mu] g / mL, the final concentration of platelet lysate is 5-15% (v / v), and the final concentration of glutamate enzyme is 0.1-1 mmol / L. The bone marrow cell culture medium can efficiently support in-vitro proliferation of bone marrow cells, through specific combination and appropriate concentration control of the culture medium, the medium-term cell acquisition amount can be remarkably increased, and medium-term cell chromosomes are complete in morphology and good in dispersity; the standard requirements of clinical bone marrow cell chromosome karyotype analysis, blood disease diagnosis and cell genetics experiments in the scientific research field can be met.

The application discloses a bovine respiratory pathogen preservation solution and a preparation method and application thereof, and belongs to the technical field of biological body preservation reagents. The bovine respiratory pathogen preservation solution provided by the application comprises appropriate amounts of carboxymethyl cellulose sodium, gentamicin, fetal bovine serum, trypsin, phosphate and sodium chloride and the like. The bovine respiratory pathogen preservation solution can effectively protect the integrity of the bovine respiratory pathogen under normal temperature conditions, can prevent bacterial contamination, is beneficial to subsequent bovine respiratory pathogen separation, culture and identification, and thus the distribution and infection of the bovine respiratory pathogen can be correctly detected, and then targeted prevention and treatment measures can be taken.

This invention discloses a combination of Western and traditional Chinese medicine for treating gastric ulcers. The combination comprises ① gentamicin (80,000 units / vial) and a combination of gentamicin and a remedy called "Weidele". This combination has anti-inflammatory, laxative, and anti-inflammatory effects when taken orally. It is effective in treating inflammation and is safe to use with an extremely low recurrence rate. Improvement is seen within fifteen days, with no obvious toxic side effects. After approximately ten days of symptomatic treatment with the combination of gentamicin (80,000 units / vial) and remedyle, the condition improved, symptoms disappeared, and a follow-up gastroscopy showed improved lesion absorption. Continued anti-inflammatory treatment and a follow-up gastroscopy at three months were then performed.

The invention provides a beneficial bug feed and a preparation method thereof. The feed comprises 40000 mg of pork liver, 40000 mg of tuna, 5000 mg of tenebrio molitor powder, 65000 mg of egg yolk, 17000 mg of corn germ oil, 5000 mg of pure milk, 0.04 mg of biotin, 2 mg of calcium pantothenate, 100 mg of choline chloride, 0.5 mg of folic acid, 40 mg of muscle sugar, 2 mg of nicotinamide, 0.5 mg of pyridoxine, 0.5 mg of thiamine, 1 mg of riboflavin, 4 mg of cobalamin, 540 mg of ascorbic acid, 40 mg of p-aminobenzene methylamine, 40 mg of nicotinic acid, 1000 mg of L-glutamine, 6000 mg of sucrose, 6.2 mg of gentamicin, 4000 mg of casein and 400 mg of sorbic acid. The method comprises the following steps: weighing the components in proportion, mincing the pork liver, the tuna and the egg yolk, boiling until the color is changed, cooling to room temperature, adding the remaining components, supplementing deionized water to 250 ml, and uniformly mixing. The artificial feed has the advantages of easily available ingredients, simple preparation, low cost and time and cost saving, and can be used as a breeding temporary substitute feed or a field supplement feed.

The application discloses a doramectin monoclonal antibody, a detection test strip and application thereof, and relates to the field of animal health. 50 The heavy chain and light chain variable regions of the monoclonal antibody have specific complementarity determining region sequences, and the half inhibition concentration IC of the monoclonal antibody to doramectin is as low as 3-5 ng / mL, which shows excellent sensitivity; the cross reaction rates of the monoclonal antibody to abamectin, ivermectin and acetyl abamectin are 75%, 75% and 60% respectively, and the monoclonal antibody has no cross reaction with common antibiotics such as tetracycline and gentamicin. Based on the antibody, the application also develops a colloidal gold immunochromatography test strip, which is simple in operation, rapid in detection, visual in result, and has a detection limit of 5 ng / mL, and is suitable for on-site rapid screening of doramectin and abamectin drug residues in animal-derived food, and has a good application prospect.

Provided are a tetragenococcus halophilus with an inhibitory effect on bacillus cereus and a use thereof. The preservation number of the tetragenococcus halophilus is GDMCC 64945. The tetragenococcus halophilus is isolated from fermented bean curd, and colonies of the tetragenococcus halophilus are milky white, small and round on a solid medium; The tetragenococcus halophilus is Gram-positive, and when observed under a microscope, the morphology thereof is mostly diplococci or tetrads, with no chain-like formations. The strain is sensitive to eight antibiotics such as ampicillin, gentamicin and kanamycin, and has no drug resistance gene and virulence gene. A bacterial suspension of the strain has a good inhibitory effect on a bacillus cereus strain isolated from the fermented bean curd, and when the strain is applied to a fermented bean curd fermentation system, the growth of bacillus cereus in the system can be effectively inhibited, and the content of enterotoxin in the system is reduced.

The invention discloses a Rongchang pig source ZH30 strain and application thereof, and belongs to the technical field of agricultural livestock feed additives. The ZH30 strain provided by the invention has bacteriostatic activity on a plurality of common pig pathogenic bacteria such as escherichia coli, staphylococcus aureus, salmonella typhimurium, salmonella pullorum and enterococcus faecalis, is wide in antibacterial spectrum, and is beneficial to reducing the load of pathogenic bacteria in intestinal tracts of pigs. Meanwhile, the ZH30 strain is sensitive to streptomycin, gentamicin and ampicillin, infectious microbe pollution can be conveniently controlled through a conventional antibiotic means in the fermentation and preparation production process, and meanwhile the drug resistance spreading risk is reduced. As a functional strain of a probiotic preparation for pigs, the ZH30 strain disclosed by the invention can improve the intestinal health of pigs and improve the mechanical barrier integrity of the intestinal tracts, has potential application values of reducing the diarrhea risk of piglets and improving the feed utilization rate and the production performance, and is of great significance to the development of animal husbandry and feed industry.

The present invention relates to a pharmaceutical composition for the prevention or treatment of hearing loss containing ammonium lactate as an active ingredient. It has been confirmed that treating hair cells damaged by gentamicin with ammonium lactate increases the number of normal hair cells, and therefore, a pharmaceutical composition containing ammonium lactate as an active ingredient can be used to prevent or treat hearing loss, a side effect of using the antibiotic ammonium lactate.

This invention, entitled "A Fertilization Culture Medium, Its Preparation Method, and Its Application," belongs to the field of assisted reproductive technology. The technical problem it aims to solve is that existing technologies, due to the generation of sperm DNA fragments during the culture process, lead to a decrease in overall sperm quality, limiting fertilization rate and blastocyst formation rate. This invention proposes a fertilization culture medium that can improve overall sperm quality and thus increase fertilization rate. The key technical points are: a fertilization culture medium comprising the following components: sodium chloride, potassium chloride, alkali metal dihydrogen phosphate, calcium chloride, magnesium sulfate, sodium bicarbonate, sodium lactate, sodium pyruvate, glucose, fructose, disodium edetate, gentamicin, human serum albumin, alanylglutamine, taurine, non-essential amino acids, and testosterone.