61 results about "Cobalamin" patented technology

The invention discloses a fermentation medium for regulating and controlling a clostridium aerovorans target product and a fermentation method of the clostridium aerovorans target product. The medium comprises a basic component and a regulating component, the basic components comprise ammonium chloride, potassium chloride, magnesium sulfate heptahydrate, sodium chloride, monopotassium phosphate, yeast powder, calcium chloride dihydrate, sodium selenite, nickel chloride hexahydrate, biotin, folic acid, pyridoxine hydrochloride, thiamine, riboflavin, nicotinic acid, D-calcium pantothenate, cobalamin, para aminobenzoic acid and lipoic acid; the adjusting component comprises sodium tungstate, ferrous sulfate and sodium molybdate, sodium tungstate and ferrous sulfate have a forward adjusting effect on increasing the yield of acetic acid, ethanol and butanol in the target product, and sodium molybdate has a reverse adjusting effect on the target product. By optimizing the proportion of trace elements in the culture medium, the clostridium metabolism efficiency is improved, synthesis of a target product is regulated and controlled, and the method is particularly suitable for industrial production of acetic acid, ethanol, butanol and the like.

The invention relates to the field of environmental microbial remediation, in particular to a strain of trichlorobacterium and application thereof in bioremediation of pollutants. According to the present invention, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, and the bacillus subtilis is bacillus subtilis. The strain NB-12 obtained by the invention is a strain of self-synthesized cobalamin screened from river bottom sediments, is suitable for extensive industrial production and degrades a plurality of halogenated alkanes in the environment.

The invention discloses application of cobalamin and beneficial bacteria in prevention and control of tomato soil-borne bacterial wilt. The beneficial bacterium is Chryseobacterium CHR6, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) The invention discloses a synbiotics for preventing and controlling tomato soil-borne bacterial wilt. The synbiotics consist of beneficial bacteria and cobalamin, and the beneficial bacterium is Chryseobacterium CHR6. The invention discloses a method for preventing and controlling tomato soil-borne bacterial wilt by matching cobalamin and beneficial bacteria, which comprises the following steps: 7-10 days after transplanting tomato seedlings, adding synbiotics into soil by a root irrigation method, adding 10-20 mL of cobalamin solution with the concentration of 5-10 nM into each tomato seedling, and inoculating 10-20 mL of beneficial bacteria suspension with the concentration of 1 * 10 < 7 >-1 * 10 < 9 > CFU / mL. By adding cobalamin, inhibition of growth of ralstonia solanacearum through siderophore mediation of Chryseobacterium CHR6 can be remarkably enhanced, and occurrence of tomato soil-borne bacterial wilt is reduced.

A medium supplement for high-yield culture of anaerobes is disclosed. The medium supplement includes N-acetylhexosamine, L-aspartic acid, L-cysteine and cobalamin. A culture medium including the supplement and a culture method using the culture medium are also disclosed. It is possible to provide an innovative method which is capable of achieving high-concentration culture of anaerobes that are difficult to culture in high yield. The method is cost-effective, and in particular, is capable of culturing large amounts of fastidious aerobes suitable for use in food and pharmaceutical applications.

The application provides a method for detecting the titer of vitamin B12 in a fermentation liquor. The method comprises the following steps: preparing a potassium dichromate solution, a hydrochloric acid solution and a potassium dihydrogen phosphate solution, mixing the three solutions with the vitamin B12 fermentation liquor under certain conditions to generate cyanocobalamin, and then testing the titer of the cyanocobalamin by using a high performance liquid chromatography. The titer of the vitamin B12 detected by using the method is consistent with the titer detected by using a sodium cyanide method. The operation method greatly reduces the safety risk of an analyst caused by using the sodium cyanide, and the operation method is simple, easy to manage in a laboratory and can save the testing cost.

This application provides a barrier-repairing composition and its use. The composition comprises panthenol, niacinamide, folic acid, and cobalamin in a mass ratio of 3-7:1-5:0.0005-0.1:0.00005-0.01. This application also provides uses of the composition and products containing the same. The composition utilizes panthenol, niacinamide, folic acid, and cobalamin in combination, where the components complement and enhance each other, achieving a synergistic effect in upregulating BMAL2 gene expression. This enhances the DNA damage repair capacity of skin fibroblasts and potentially promotes barrier repair. Furthermore, the composition provided herein can significantly reduce the expression of capsaicin receptor 1 (TRPV1) and increase the expression of involucrin, PIEZO1, and TNXB, reducing skin discomfort and enhancing skin barrier function. Furthermore, the composition provided herein can reduce the expression of inflammatory factors, demonstrating its anti-inflammatory effect.

The invention provides a rumen bypass folic acid additive, a preparation method and application thereof and a method for producing milk rich in folic acid, and particularly belongs to the technical field of rumen bypass additives. The rumen bypass folic acid additive comprises folic acid, cobalamin, silicon dioxide, lipase, palm fat, coconut oil and calcium stearate. The rumen bypass folic acid additive can reduce folic acid degradation by rumen microorganisms, can directly reach small intestines to be absorbed, improves the folic acid utilization rate of ruminants, specifically can improve the milk yield, milk protein, milk fat and folic acid content and yield of dairy cows, and can produce folic acid-rich cow milk; the feed can also increase the plasma glucose level and reduce the concentration of beta-hydroxybutyric acid, so that the energy metabolism state of the dairy cow is improved, the production performance of the dairy cow is improved, and economic benefits are brought to pastures.

The main purpose set by the present inventors is to provide a technology for suppressing inflammation particularly in aged tissue in the oral cavity.　The present inventors have discovered that it is possible to suppress inflammation particularly in aged tissue in the oral cavity by an oral cavity composition that contains β-glycyrrhetinic acid and at least one selected from the group consisting of retinol acetate, retinol, all-trans-retinoic acid, ascorbic acid, cobalamin, calciferol, anthraquinone, L-histidine, methylhesperidin, and chemically possible salts thereof.

The invention belongs to the field of animal nutrition and feed science, and relates to a feeding technology capable of improving production performance, body health (immunity and oxidation resistance) and economic benefit of mutton sheep and improving rumen pH value, blood buffer capability and blood acid-base balance maintaining capability, namely preventing chronic rumen acidosis, and a daily ration preparation method thereof. According to the feeding technology and the daily ration preparation method, vitamin B12 (cyanocobalamine) is added into the mutton sheep high-concentrate daily ration (the concentrate-to-coarse ratio is 6: 4), the adding level is 0.5 mg / kg in terms of dry matter per kg of the daily ration, it is at least guaranteed that the pre-feeding period is 7 days and the positive test period is 30 days or above in the feeding time of the mutton sheep, the production performance, the organism immunity, the oxidation resistance and the economic benefits of the mutton sheep can be improved, and the mutton sheep feed is suitable for large-scale production. The pH value of the rumen, the blood buffering power and the blood acid-base balance maintaining capacity are improved, namely the effect of preventing chronic acidosis of the rumen is achieved, and the mutton sheep feed additive is suitable for mutton sheep with the weight being 34 kg or above. The method is easy to operate and safe in production, and a new thought and method are provided for preventing chronic rumen acidosis under the condition that mutton sheep are fed with high-concentrate daily ration.

The present invention relates to a nutritional vitamin complex for pets intended to improve the fur and skin quality of pets such as dogs and cats. The composition of the complex contains omega-3, curcumin, piperine, biotin (vitamin H or vitamin B7), vitamin B2 (riboflavin), vitamin B3 (nicotinic acid or niacin), vitamin B5 (pantothenic acid), vitamin B6 (pyridoxine), vitamin B12 (cyanocobalamin), folic acid (vitamin B9), vitamin E (alpha-tocopherol), vitamin C, zinc (zinc glycine chelate), coenzyme Q10, hydrolysed type II collagen, astaxanthin, DL-methionine, selenium, L-glycine, L-proline, L-phenylalanine, L-leucine, L-isoleucine, L-histidine, L-arginine, L-lysine, L-tyrosine, resveratrol, and a flavouring agent.

The invention relates to a preparation method of hydroxycobalamin salt, which comprises the following steps: carrying out reduction reaction on cobalamin adenosine fermentation liquor before photochemical reaction, carrying out macroporous resin separation and anion resin decoloration, and finally carrying out crystallization treatment to obtain the hydroxycobalamin salt. According to the method, the yield and the purity of the product are effectively improved, related substances of the product are reduced, the use of cyanide is avoided in the production process, the method is environment-friendly, the amount of wastewater is small, and green and environment-friendly industrial production of the hydroxycobalamin salt is easy to realize.

The present invention relates to a nutritional vitamin complex for pets intended to improve the fur and skin quality of pets such as dogs and cats. The composition of the complex contains omega-3, curcumin, piperine, biotin (vitamin H or vitamin B7), vitamin B2 (riboflavin), vitamin B3 (nicotinic acid or niacin), vitamin B5 (pantothenic acid), vitamin B6 (pyridoxine), vitamin B12 (cyanocobalamin), folic acid (vitamin B9), vitamin E (alpha-tocopherol), vitamin C, zinc (zinc glycine chelate), coenzyme Q10, hydrolysed type II collagen, astaxanthin, DL-methionine, selenium, L-glycine, L-proline, L-phenylalanine, L-leucine, L-isoleucine, L-histidine, L-arginine, L-lysine, L-tyrosine, resveratrol, and a flavouring agent.

This invention belongs to the field of detection technology and relates to a highly sensitive method for quantitative analysis and structural identification of cobalamin, as well as its application. Using three common natural cobalamins with different structures found in the environment as standards, a full-scan, automatically triggered secondary mass spectrometry acquisition method is employed to obtain the cobalamin precursor ion and a series of high-abundance characteristic ion fragments. Based on the principle that fragments of the same cobalamin have the same fragment ion m / z and the fragment ion search function of the software, the structure of unknown cobalamin is identified. Then, a parallel reaction monitoring mass spectrometry quantitative method is used. A large number of interfering ions are filtered out by a quadrupole, and the cobalamin low-position ligand fragment ions with the highest abundance and high identifiability are used as quantitative ions to analyze the type and content of cobalamin in the sample. This invention, by combining two data acquisition methods of high-resolution mass spectrometry, can effectively eliminate interference from the endogenous matrix, improve the sensitivity of sample analysis, and enhance the specificity of quantification, thereby achieving highly sensitive detection of different types of cobalamin.

The invention relates to a photo-enzyme catalytic reaction for producing methane through oxygen demethylation of aryl methyl ether and application of the photo-enzyme catalytic reaction. According to the method, demethylase MTase I and cobalamine binding protein CP which are purified by a nickel column are utilized, the demethylase MTase I, the cobalamine binding protein CP and 5-denitrified riboflavin react with different aryl methyl ether substrates respectively in a buffer solution with the pH value of 6.5 under the conditions of purple light irradiation and anaerobic conditions, O-demethylation of aryl methyl ether is catalyzed, and the 5-denitrified riboflavin is obtained. Demethylation products of methane and phenols are generated; according to the method disclosed by the invention, demethylation methane production reaction is completed, and meanwhile, the reaction efficiency and the substrate spectrum of oxygen demethylation of aryl methyl ether are improved. In-vitro catalysis of the demethylation methane production reaction of flavone aryl ether and natural biomass lignin alkaline hydrolysate is realized for the first time, and compared with the current demethylation reaction, the method has the advantages of mild reaction conditions, environmental protection and the like, and meanwhile, the removed methyl is converted into methane, so that the method is suitable for industrial production. The addition of additional methyl acceptors and the generation of byproducts are effectively avoided, and the atom economy is improved.

The invention discloses a formula and a preparation method of a disease-preventing health-care oral liquid. The health-care food is prepared from the following raw materials in parts by weight: 4 to 6 milliliters of purple perilla seed oil, 5 to 7 milliliters of walnut oil, 100 to 150 milligrams of algal oil, 200 to 250 milligrams of grape seed extract, 50 to 80 milligrams of selenium-enriched yeast, 80 to 120 milligrams of vitamin C, 80 to 120 milligrams of vitamin E, 0.6 to 1.0 milligram of vitamin B1 (thiamine) and 0.6 to 1.0 milligram of vitamin B2 (riboflavin). After being taken by multiple persons for a period of time, the traditional Chinese medicine composition is proved to be safe to take, free of adverse reaction and obvious in health care effect, old people feel weak in action, heavy in whole body, dry in mouth and throat and insomnia and constipation before taking the traditional Chinese medicine composition, symptoms are relieved after taking the traditional Chinese medicine composition, and the traditional Chinese medicine composition is safe to take, free of adverse reaction and obvious in health care effect. Young people are good in working state and honor in spirit, and children are fast in learning progress, good in taste, high in adaptability and beneficial to popularization, and can play a positive role in prevention before illness.

This invention discloses a strain of *Lactobacillus paracasei* capable of converting cyanocobalamin and promoting its absorption, thereby enhancing brain nerve repair and improving cognition, and its postbiotic, belonging to the fields of microbial technology and pharmaceutical technology. The *Lactobacillus paracasei* CCFM1527 and its prepared postbiotic provided by this invention promote the growth of vitamin B1 in the host. 12 The neuroprotective effects of upregulating the expression of absorption receptors and converting CN-CbI to methylcobalamin include: (1) promoting the upregulation of Amnionless expression in intestinal cells (Caco-2); (2) converting CN-CbI to methylcobalamin; and (3) promoting the upregulation of Amnionless expression in mouse ileum. AMN Upregulation of gene expression (4) significantly increased the levels of vitamin B in mice. 12 (5) Significantly promotes the expression of MBP and PSD-95 proteins in mice; (6) Improves the decline in cognitive ability in mice, and has great application prospects.

The invention provides a coated cobalamin additive as well as a preparation method and application thereof, and belongs to the technical field of animal additives. According to the coated cobalamin additive provided by the invention, the cobalamin is reasonably coated, so that the cobalamin has certain stability in rumen, and most of the cobalamin is ensured to be quickly released in small intestines, so that the absorption amount of an organism is increased. The coated cobalamin additive can reduce the waste of cobalamin in rumen and improve the absorption and utilization rate of cobalamin in small intestines. The feed cost can be reduced, and the utilization efficiency of ruminants on other nutritional ingredients in the feed can be improved.

This invention belongs to the field of biotechnology and discloses a strain that produces vitamin B. 12 This invention relates to *Propionibacterium ferruginea* with anti-anemia function, its post-biotics, preparations, methods, and applications. The strain is named S2A35, classified as *Propionibacterium ferruginea*, with accession number CGMCC No. 37757, accession date February 9, 2026, and deposited at the China General Microbiological Culture Collection Center (CGMCC), No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. This *Propionibacterium ferruginea* strain is capable of producing active vitamin B. 12 This strain has the function of cobalamin, and both the live bacteria and its post-biotics have the function of treating anemia. It is expected to be used for vitamin B12. 12 Its production, as well as its use in preparing foods, health products, foods for special medical purposes, and medicines for the prevention, improvement, and treatment of anemia, have very broad application prospects.

The invention discloses a formula of an organic mixed health-care beverage and a preparation method of the organic mixed health-care beverage. The raw materials comprise, by weight, 1-3 g of ginseng, 1-3 g of deer-horn glue, 2-5 g of folium cortex eucommiae, 2-5 g of gynostemma pentaphylla, 2-5 g of hawthorn, 2-5 g of ligusticum wallichii, 1-3 g of schisandra chinensis, 3-5 g of rhizoma polygonati, 2-5 g of radix ophiopogonis, 100-200 mg of algal oil, 200-300 mg of taurine, 150-200 mg of grape seed extract, 3-5 ml of perilla seed oil, 50-80 ml of yoghourt, 30-50 ml of walnut juice and 30-50 ml of peanut liquid. The selenium-enriched beverage is prepared from 30 to 50 milliliters of sweet almond liquid, 30 to 50 milliliters of black sesame liquid, 30 to 50 milliliters of mango juice, 50 to 100 milliliters of apple juice, 30 to 50 milliliters of tomato juice, 50 to 80 micrograms of selenium-enriched yeast, 25 to 50 milligrams of vitamin C, 125 to 25 milligrams of vitamin E, 0 to 15 milligrams of vitamin B1 {thiamine}, 15 milligrams of vitamin B2 {riboflavin}, 0 to 15 milligrams of vitamin B2 {pyridoxine}, 15 milligrams of vitamin B6 {pyridoxine}, 25 milligrams of vitamin B9 {folic acid}, 40 micrograms of vitamin B9 {folic acid}, and 0 to 75 milligrams of vitamin B12 {cobalamin}. After being taken by various people for a period of time, the health-care food is smooth and delicious in taste and free of adverse reaction through evaluation in multiple aspects, and the health-care effect and feeling state of the health-care food are better than those of the prior art. And the health-care tea is suitable for a wide range of people, is beneficial to market promotion, and enables more people to achieve the health-care effect of preventing diseases before diseases.

To provide cosmetics that are aesthetically superior by suppressing discoloration over time and the precipitation of ingredients contained in the cosmetics. [Solution] By including (A) hydrolyzed gardenia extract, (B) cyanocobalamin, and (C) cyclohexane-1,4-dicarboxylic acid bisethoxydiglycol, the cosmetic composition can suppress discoloration over time and precipitation of components contained in the cosmetic composition, resulting in superior aesthetics. The cosmetic composition may also contain (A) hydrolyzed gardenia extract in an amount of 0.0005 to 0.05% by mass, (B) cyanocobalamin in an amount of 0.0005 to 0.005% by mass, and (C) cyclohexane-1,4-dicarboxylic acid bisethoxydiglycol in an amount of 0.05 to 0.5% by mass.

This invention provides a pharmaceutical composition combining azelastine and methylcobalamin. [Solution] An orally administered pharmaceutical composition is provided, comprising azelastine or a pharmaceutically acceptable salt of azelastine, methylcobalamin, and one or more pharmaceutically acceptable excipients, wherein the pharmaceutical composition is formulated as a capsule, caplet, tablet, or pill configured for oral administration and absorption in the gastrointestinal tract, and the azelastine or a pharmaceutically acceptable salt of azelastine and methylcobalamin are present together in the pharmaceutical composition in a therapeutically effective daily dose for treating Alzheimer's disease or Parkinson's disease in human patients.

The present invention provides a novel food and drink containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin. According to an embodiment of the present invention, there is provided a bottled beverage containing carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the content of carnosine is 0.70-15 mg / mL and the content of the at least one cobalamin is 0.005-1 µm / mL.

The present application discloses a methylcobalamin tablet, which contains, by weight ratio: 0.8 - 1.2 parts of mecobalamin, 15 - 18 parts of starch, 55 - 65 parts of microcrystalline cellulose, 100 - 110 parts of lactose, 0.4 - 0.5 parts of calcium stearate, and 8 - 10 parts of coating agent. Among them, the particle size range of the mecobalamin after micronization is 0.60μm ≤ D10 ≤ 0.90μm, 5.2μm ≤ D50 ≤ 6.3μm, 20μm ≤ D90 ≤ 26.5μm, and the particle size range of the lactose after micronization is 1.0μm ≤ D10 ≤ 2.5μm, 6.5μm ≤ D50 ≤ 10.5μm, 23μm ≤ D90 ≤ 35μm.

The present invention relates to a combination of a vitamin B selected from cobalamin and or vitamin B6, and a medium chain fatty acid or a salt thereof, and compositions comprising the combination.

The erythromycin fermentation culture medium is optimized by comparing transcriptomics analysis. Specifically, the invention relates to a method for optimizing the fermentation yield of erythromycin by adding six vitamins into a culture medium in a combined manner. More specifically, the six vitamins comprise thiamine pyrophosphate, riboflavin, pyridoxine, folic acid, cobalamin and hemin. The vitamin is added into the fermentation culture medium, so that the growth of erythromycin producing bacteria in the fermentation culture medium is promoted, and the yield of erythromycin is effectively increased.

The disclosed invention relates to a chemical composition that can be taken orally and supplements an unhealthy diet and lifestyle. In one aspect of the invention the chemical composition includes Retinol Palmitate, Thiamine, Riboflavin, Niacin, Nicotinamide mononucleotide (NMN), Pyridoxal, Folic Acid, Cyanocobalamin, Cholecalciferol, Zinc, Manganese, Copper, Calcium, Magnesium, Iron, L-Selenomethionine, Lysine, Phenylalanine, L-Threonine, L-Methionine, L-Histidine, L-Tryptophan, L-Leucine, L-Isoleucine, L-Valine, Cinnamon Bark Powder, and Curcumin.

The invention provides an anti-inflammatory repair composition containing various B vitamins and cornflower extract and application. The anti-inflammatory repair composition comprises panthenol, nicotinamide, folic acid, cobalamin and cornflower extract. According to the composition, the panthenol, the nicotinamide, the folic acid, the cobalamin and the cornflower extract are compounded for use, the components are mutually matched and promoted, and a synergistic effect is achieved in the aspect of up-regulation of expression of two key factors, namely FGF2 and CAT, so that the effect of promoting skin repair is achieved. In addition, the composition provided by the invention can also significantly reduce expression of inflammatory factors, such as IL-8, and has a stronger effect of inhibiting inflammation.

The invention provides a barrier repairing composition and application. The composition is prepared from panthenol, nicotinamide, folic acid and cobalamin according to the mass ratio of (3 to 7) to (1 to 5) to (0.0005 to 0.1) to (0.00005 to 0.01). The invention also provides application of the composition and a product comprising the composition. According to the application, the panthenol, the nicotinamide, the folic acid and the cobalamin are compounded for use, all the components are mutually matched and promoted, and a synergistic effect is obtained in the aspect of up-regulating BMAL2 gene expression, so that the DNA damage repair capability of skin fibroblasts can be enhanced, and a potential barrier repair promoting function is played. Meanwhile, the composition provided by the invention can significantly reduce the expression of capsaicin receptor 1 (TRPV1), improve the expression of endoprotein, PIEZO1 and TNXB, reduce the skin discomfort reaction and make the skin barrier function more stable. In addition, the composition provided by the invention can reduce expression of inflammatory factors and has an effect of inhibiting inflammation.

A beverage composition for hydration and for supporting head hair health is described. The beverage composition includes (i) amla and (ii) one or more head hair benefit ingredient(s). Head hair benefit ingredient(s) in the beverage composition may include trace elements such as zinc, calcium, and selenium, among others; and vitamins such as: vitamin A; vitamin B, e.g., riboflavin, biotin, folate, thiamine, niacin, pantothenic acid, pyridoxine, and cobalamins, etc.; vitamin C; vitamin D; vitamin E; and vitamin K. The beverage compositions of the present disclosure in addition to supporting head hair health and promoting growth of head hair have also been found to be beneficial to support health of skin and nails.

The present invention relates to over the counter (OTC) film coated tablet compositions comprising one or more types of vitamins. Specifically, the invention focuses on formulations that include B vitamins, such as thiamine (B1), riboflavin (B2), nicotinamide (B3), pantothenic 5 acid (B5), pyridoxine (B6), biotin (B7), folic acid (B9), and cobalamin (B12). These vitamin- enriched tablets are intended for dietary supplementation to support various bodily functions, including energy metabolism, nervous system health, and the maintenance of healthy skin, hair, and eyes.