29 results about "Rumen microorganisms" patented technology

The invention discloses a multifunctional cellulolytic enzyme and application thereof in degradation of agricultural by-products, and belongs to the technical field of biology. The multifunctional cellulolytic enzyme is named RuCelI, and the amino acid sequence of the multifunctional cellulolytic enzyme is as shown in SEQ ID NO. 1. Based on a rumen microbial metagenome resource mining technology, a novel multifunctional cellulose decomposition enzyme RuCelI is developed, and the enzyme has double activities of endo-cellulase and xylanase, can be used for degrading agricultural by-products, reducing the treatment cost and improving the degradation efficiency, and is beneficial to improving the utilization rate and the economic benefit of the agricultural by-products.

The invention relates to the technical field of resources and environment, in particular to a method for pretreating lignocellulose by using a rumen microorganism dry method. The method comprises the following steps: crushing lignocellulose to 1-5cm, and filling the crushed lignocellulose into a sealed space; spraying microorganisms under an anaerobic condition according to a mass ratio of rumen microorganisms to lignocellulose volatile components of (0.015-0.03): 1, and controlling the water content of the system to be 30-60%; the pretreatment temperature is regulated and controlled to be 35-42 DEG C, the pH is 6.0-7.5, the pretreatment time is 10-14 days, so that the total abundance of the functional microorganisms Pseudocidovorax, Hallella and Prevotella is greater than or equal to 55%, and the three types of microorganisms respectively form strong interaction with specific companion bacteria; when the relative crystallinity of the lignocellulose is reduced by more than 15% and the volatile component is reduced by 10%, finishing the pretreatment; and performing process regulation and control according to the crystallinity and the reducing sugar yield when the pretreatment is performed for 10 days. The dry process is adopted, the bionic principle and the solid-state fermentation technology are combined, all-component gradient utilization of lignocellulose is achieved, a green solution is provided for industrial and high-value conversion of lignocellulose, and remarkable economic, social and environment-friendly benefits are achieved.

The invention discloses a method for predicting the sperm motility of a breeding bull based on a host SNP-rumen microorganism-metabolite interaction axis, and the sperm motility of the breeding bull can be obtained by detecting the haplotype condition of SNP1-SNP3 and / or SNP4-SNP6 sites of a breeding bull genome. The method breaks through the limitations of strong dependency on phenotype endpoint evaluation and prediction lag in the current bull seed selection process, and realizes forward accurate prediction and early evaluation of the semen quality of the breeding bull.

This invention discloses a methanogenic lyase homologous to PeiR lyase and its applications. The PeiR lyase protein, targeting the peptide bonds of the methanogenic cell wall, participates in the biological process of hydrolyzing the archaea cell wall, effectively killing methanogens and reducing methane production. Proteins homologous to this lyase possess potential methane-reducing potential. This invention utilizes a series of bioinformatics software to identify protein sequences encoded by methanogenic viruses from a large amount of rumen microbial virome sequencing data, and analyzes the structural homology between these proteins and PeiR. Ultimately, this invention successfully obtained a series of methanogenic lyases homologous to PeiR. To achieve the expression of these lyases, this invention designed multiple primers to synthesize the target sequences and successfully expressed them in a prokaryotic expression system. In vitro gas production experiments with the crude enzyme solution showed that these lyases are effective in reducing methane production.

The invention discloses a novel GAA enzyme for improving the degradability of lignocellulose and application of the novel GAA enzyme, the GAA enzyme is derived from 1, 4-benzoquinone reductase of an AA6 family of ruminant rumen microorganisms, and the amino acid sequence of the GAA enzyme is shown as SEQ ID NO.1. Experiments prove that the enzyme has high catalytic activity and stability in a rumen environment (pH 6.0-6.8, 39 DEG C), can effectively improve the degradation rate of lignocellulose, and has wide application prospects in preparation of enzyme preparations, feed additives or microecologics for improving the utilization rate of lignocellulose in ruminant roughage.

The invention belongs to the technical field of livestock feed, and particularly discloses a total mixed ration containing fermented cotton straw and application thereof. The total mixed ration comprises the following raw materials on the basis of dry matter: 5-15% of fermented cotton straw, 10-20% of corn straw, 5-10% of alfalfa, 25% of corn, 9% of soybean meal, 7.5% of dry corn vinasse, 10% of corn germ meal, 15% of soybean hull, 0.9% of calcium hydrophosphate, 0.4% of ammonium chloride, 0.2% of choline chloride, 0.5% of sodium chloride and 0.5% of sodium bicarbonate. The total mixed ration can significantly improve the growth performance and feed conversion efficiency of the Hu sheep, improve the digestion and utilization rate of nutrient substances, effectively improve the health and anti-stress level of animal bodies, improve the diversity and structure of rumen microflora, promote the growth of beneficial microorganisms, and establish a more stable and efficient rumen fermentation system.

The application discloses a multifunctional fiber-degrading enzyme and application thereof, and belongs to the technical field of biology. The multifunctional fiber-degrading enzyme is named RuCelF, and the amino acid sequence is shown as SEQ ID NO. 1. Based on rumen microbial metagenomic resource mining technology, a new multifunctional fiber-degrading enzyme RuCelF is developed. The enzyme has double activities of cellulase and xylanase, can be used for degrading agricultural waste, reduces treatment cost, improves degradation efficiency, helps to improve the utilization rate and economic benefits of agricultural waste, and provides a new enzyme source and technical support for efficient resource utilization of agricultural waste.

The present application relates to the field of cosmetic technology, and discloses a preparation process of cashmere goat bone-derived elastin and application thereof in cosmetics. The process comprises: defatting cashmere goat bone particles; using composite rumen microbial flora to perform bionic fermentation to destruct bone matrix and expose active sites; adopting neutral protease and specific elastase to perform stepwise directional enzymolysis; performing hydrophilic modification through transglutaminase catalysis of covalent anchoring of polyglutamic acid and elastin polypeptide; and finally, performing multi-stage membrane interception purification and drying. The present application realizes complete retention of the natural cross-linking structure of elastin under mild conditions, significantly improves water solubility, biological activity and transdermal permeability of the elastin, solves the problems of structural damage and pollution caused by traditional chemical methods, and provides a green path for high-value utilization of cashmere goat by-products.

The invention discloses xylanase and application thereof, and belongs to the technical field of biology. The xylanase is named as RuXynJ, and the amino acid sequence of the xylanase is as shown in SEQ ID NO. 1. The novel xylanase RuXynJ is developed on the basis of a rumen microbial metagenome resource mining technology, has xylanase activity, can be used for degrading agricultural wastes, reducing the treatment cost and improving the degradation efficiency, is beneficial to improving the utilization rate and economic benefits of the agricultural wastes, and has a wide application prospect. And a basic support is provided for efficient resource utilization of agricultural wastes.

The invention discloses a difunctional expansin protein mutant as well as a coding gene and application thereof, and belongs to the technical field of biological genetic engineering. According to the invention, a protein PFLoos1 with bifunctional activity is obtained from a metagenome of rumen microorganisms, and mutants PFLoos1-S1, PFLoos1-S2 and PFLoos1-S3 are obtained by carrying out site-specific mutagenesis on key amino acids in a substrate binding pocket or a surface hydrophobic region of the protein PFLoos1. The mutant provided by the invention has the function of inhibiting the metabolic activity of rumen methanogenic archaea on the basis of keeping the lignocellulose degradation promoting capability. The mutant provided by the invention not only can be applied to preparation of an enzyme preparation for promoting cellulose degradation, but also can be applied to a feed additive for inhibiting ruminant rumen methane generation, and has important practical value for improving feed energy utilization efficiency and low-carbon breeding of animal husbandry.

The application relates to a microbial preparation passing through the rumen, a preparation method and application thereof. The microbial preparation passing through the rumen comprises microbial powder and a coating material coated on the surface of the microbial powder. The coating material comprises protective fat. The total weight of fatty acids in the microbial preparation passing through the rumen is 100%, the fatty acids comprise 70wt%-80wt% of palmitic acid and stearic acid, <=9wt% of oleic acid, 5wt%-15wt% of polyunsaturated fatty acids and a residual amount of non-target fatty acids, the mass ratio of the palmitic acid to the stearic acid is 1:1-2:1, the polyunsaturated fatty acids are selected from at least one of docosahexaenoic acid, eicosatetraenoic acid and eicosapentaenoic acid, and the mass ratio of the microbial powder to the protective fat is 2:3-3:2. The microbial preparation passing through the rumen described in the application is used as a feed additive for feeding ruminants such as cows, and can realize the dual improvement of the production performance of the cows and the nutrition strengthening of the milk.

The application provides application of a grifola ferroxida ferment in preparation of a feed additive for reducing methane emission of a dairy cow. The grifola ferroxida ferment is obtained by liquid submerged fermentation or liquid-solid two-phase fermentation with grifola ferroxida as an object. After the grifola ferroxida ferment is fed to the dairy cow, the activity of a fiber-decomposing bacterial group can be enhanced, the feed digestion rate is improved, the abundance of some bacteria in a rumen is improved, the activity composition of a rumen microbial population is changed, and the methane emission of the dairy cow is reduced.

The present application relates to the technical field of biomarkers, and particularly relates to a biomarker related to lactation performance of ruminants and application thereof. The marker is one or more of Prevotella bryantii, Prevotella copri, Prevotella brevis and Prevotella ruminicola. The present application can assist in breeding ruminants with high milk yield and high milk fat percentage lactation phenotype by PCR detection of the abundance of the above-mentioned marker microorganisms. In addition, the above-mentioned marker microorganisms can also be applied in the preparation of a kit for detecting the lactation performance of ruminants, and have broad application prospects.

The application discloses a slow-release urea compound animal feed additive and a preparation method and application thereof, and belongs to the technical field of animal feed. The slow-release urea compound animal feed additive is prepared from the following components in parts by mass: 30-50 parts of slow-release urea, 15-25 parts of a microbial fermentation carrier, 5-10 parts of a nitrogen regulation agent, 8-15 parts of a mineral compound, 3-8 parts of a palatability improver and 1-3 parts of an anti-caking agent. The components synergistically act to solve the technical problems of the existing slow-release urea additive, such as mismatch between the nitrogen release rate and the utilization rate of rumen microorganisms of animals, poor palatability, low nitrogen utilization rate, easy environmental pollution and animal stress. The additive is suitable for the breeding of various animals, has simple preparation process, controllable cost, green environmental protection and high popularization and application value.

The invention discloses application of guar gum and xylooligosaccharide in promoting growth of cornu cervi pantotrichum of sika deer, and belongs to the technical field of animal breeding. The invention reveals that guar gum and xylooligosaccharide synergistically promote the growth of pilose antler through'microorganism-metabolism-pilose antler growth axis' for the first time, and researches find that guar gum and xylooligosaccharide not only enhance the ability of rumen microorganisms to degrade plant polysaccharides, but also promote the utilization of host-derived polysaccharides, can promote the growth of pilose antler, and can promote the growth of pilose antler. The obvious promoting effect is achieved on the pilose antler proliferation and mineralization process. The guar gum and / or xylooligosaccharide are / is added into the sika deer total mixed ration with the concentrate-to-coarse ratio of 55: 45 to feed sika deer, so that the weight of pilose antler is remarkably increased. The invention provides an effective scheme for increasing the yield of the cornu cervi pantotrichum of the sika deer, and particularly has important transformation potential in the aspects of osteoporosis prevention and treatment, bone regeneration medicine and functional feed development.

This invention discloses a feed additive for improving rumen digestion efficiency in livestock and its preparation method, belonging to the field of animal feed additive technology. It utilizes nano-magnesium oxide intercalated into bentonite particles as a feed additive to promote food absorption and improve digestion efficiency. Urea is adsorbed into zeolite, and the esterification reaction between the surface hydroxyl groups and the carboxyl groups of acrylic acid solution forms a spatial network structure of starch-grafted modified acrylic acid solution and protein supplement, further enhancing the sustained release of urea. By encapsulating composite amino acid particles within this spatial network structure, amino acid degradation in the rumen is reduced, promoting full absorption of amino acids in the small intestine, thereby improving amino acid utilization and promoting rumen microbial growth. Zeolite and bentonite synergistically reduce ammonia emissions and improve the feeding environment through the adsorption effect of the starch-grafted modified acrylic acid solution resin.

The invention discloses a method for producing ethanol by anaerobic fermentation of straws under medium and high temperature conditions, and belongs to the technical field of biomass energy conversion. According to the method, straw with high lignocellulose content is taken as a raw material, after superfine grinding and 200-mesh sieving pretreatment are carried out, a rumen microorganism stock solution (the solid content is 8%, and the inoculation amount is 8%-15% of the dry weight of the straw) from cow dung is inoculated to construct a fermentation system, no specific yeast or invertase needs to be added in the whole process, and the initial pH does not need to be regulated and controlled. Transferring the fermentation system into an anaerobic tank, introducing nitrogen with the purity of more than or equal to 99.9% for 15-20 minutes to construct an anaerobic environment, adding 30-50 mM of 2-bromoethanesulfonic acid sodium salt to inhibit methane generation, and fermenting at the constant temperature of 42-48 DEG C, wherein the ethanol content can reach 880 mg / L or above and is 885.6 mg / L at most on the first day of fermentation, and the ratio of ethanol to the total organic acid is more than or equal to 19.3%; centrifugal precipitation can be recycled as a fermentation base material, and the straw utilization rate is high. According to the method, the production cost is greatly reduced, the process is simplified, methane emission is inhibited, and the method has economical efficiency and environmental friendliness and is suitable for industrial application of large-scale production of ethanol from straw.

The invention discloses a multifunctional cellulose degrading enzyme and application thereof, and belongs to the technical field of biology. The multifunctional cellulose degrading enzyme is named RuCelF, and the amino acid sequence of the multifunctional cellulose degrading enzyme is shown as SEQ ID NO. 1. The novel multifunctional cellulose degrading enzyme RuCelF is developed on the basis of a rumen microbial metagenome resource mining technology, has double activities of cellulase and xylanase, can be used for degrading agricultural wastes, reducing the treatment cost and improving the degradation efficiency, is beneficial to improving the utilization rate and economic benefit of the agricultural wastes, and has a wide application prospect. And a new enzyme source and technical support are provided for efficient resource utilization of agricultural wastes.

The invention relates to the technical field of resources and environments, in particular to a method for domesticating rumen microorganisms to adapt to an in-vitro environment. According to the method, cellulose biomass is taken as a fermentation substrate, a buffer solution simulating saliva components of ruminants is adopted to construct a fermentation system, and rumen microflora ordered succession is guided by accurately regulating three environmental factors of in-vitro fermentation temperature, solid content and substrate carbon-nitrogen ratio. In the hydrolysis stage, fungi Wallemia, Caecomics, bacteria NK4A214group and the like form a core hydrolysis network, and lignocellulose is efficiently decomposed to generate reducing sugar; in the acidification stage, an acid production interaction network is formed by Prevotella, Sphaerochaeta, Candida, Psychrobacter and the like, and an intermediate product is converted into volatile fatty acid. According to the method, the construction of the in-vitro stable interaction network of the rumen microorganisms is realized, the yield of reducing sugar and VFAs is remarkably improved, the problems of difficult in-vitro adaptation and poor degradation and production performance of the rumen microorganisms are effectively solved, and an efficient and stable technical scheme is provided for resource utilization of cellulose biomass.

The invention discloses application of a cordyceps sinensis mycelium fermentation culture in promotion of calf growth, and belongs to the technical field of animal breeding. The cordyceps sinensis mycelium fermentation culture is obtained by performing fermentation culture on cordyceps sinensis liquid, performing solid-liquid separation, collecting a liquid part, and performing concentration and spray drying. Research finds that the cordyceps sinensis mycelium fermentation culture can effectively enhance the body resistance of calves, reduce diarrhea and cold morbidity, remodel rumen micro-ecology, improve microbial population structure, and up-regulate complex carbohydrate decomposition and galactose metabolic pathway in rumen microorganisms, so as to improve the immunity of calves. The digestion and utilization potential of the calves on nutrient substances such as fibers is remarkably improved, and protein and energy metabolism is optimized, so that the growth of the calves is promoted.

The invention discloses a ruminant rumen microorganism sampling device, and relates to the technical field of microorganism sampling, and the technical scheme is that the ruminant rumen microorganism sampling device comprises a base, a negative pressure device, a sampling bottle and a sampling pipe, the outside of the sampling pipe is movably connected with a protection plate, a guide pipe is installed on the inner side of the protection plate, and an opening groove is formed in the outside of the guide pipe; the exteriors of the guide pipe and the protection plate are jointly provided with a positioning assembly for the sampling pipe, the positioning assembly comprises a fixed plate, the inner side of the protection plate is provided with a first collection box, the inner side of the protection plate is provided with a second collection box, and the end part of the sampling pipe is provided with a sampling head. According to the sampling device, the sampling pipe is prevented from being bitten to cause blockage of the sampling pipe and influence on sampling work, meanwhile, the sampling pipe can be prevented from being damaged due to long-time biting of animals, and meanwhile, the sampling pipe can be positioned through the positioning assembly when the end part of the sampling pipe moves to a sampling position, so that sampling work is facilitated.

The application discloses application of guar gum and xylo-oligosaccharide in promoting growth of sika deer antler and belongs to the technical field of animal breeding.The application first discloses that the guar gum and the xylo-oligosaccharide promote the growth of the sika deer antler through a "microorganism-metabolism-sika deer antler growth axis".Research finds that the guar gum and the xylo-oligosaccharide not only enhance the ability of rumen microorganisms to degrade plant polysaccharides, but also promote the utilization of polysaccharides from the host source, can promote the growth of the sika deer antler, and have a significant promoting effect on the proliferation and mineralization process of the sika deer antler.Adding the guar gum and / or the xylo-oligosaccharide to a total mixed ration of the sika deer with a ratio of concentrate to roughage of 55:45 can significantly increase the weight of the sika deer antler.The application provides an effective scheme for increasing the yield of the sika deer antler, and has important conversion potential in the prevention and treatment of osteoporosis, bone regeneration medicine and the development of functional feed.

The invention discloses a method for efficiently producing volatile fatty acid by anaerobic fermentation of white spirit vinasse based on rumen microorganisms, which comprises the following steps: S1, substrate pretreatment: selecting white spirit vinasse as a fermentation substrate, and sequentially drying and crushing the fermentation substrate to obtain a pretreated substrate; s2, construction of an inoculation system: taking fresh rumen fluid as an inoculation source, mixing the fresh rumen fluid with an artificial saliva culture medium and a pretreatment substrate, adjusting the concentration of the substrate to 2-5% (w / v), and constructing a fermentation system; s3, anaerobic fermentation: placing the fermentation system in an anaerobic environment, culturing at 37-40 DEG C for 3-7 days, and maintaining the pH value of the fermentation system at 5.5-6.0 in the culture process. According to the method, efficient hydrolysis and conversion of the Baijiu vinasse are achieved, and the total yield of volatile fatty acid is remarkably increased.

The application discloses silage after pepper straw-containing feed and a preparation method and application thereof, wherein the feed is composed of the following raw materials in parts by weight: a straw particle mixture containing silage after pepper straw 45 parts, corn 28 parts, soybean meal 8 parts, corn husk 10 parts and premix 9 parts, and the proportion of the silage after pepper straw in the straw particle mixture is 20%-80%. The significant effect is that the feed can obviously promote the appetite of beef cattle and improve the production performance, can obviously improve the apparent digestibility of dry matter, crude protein and neutral detergent fiber in nutrients, increase rumen microbial protein synthesis and serum albumin content, and will not cause damage to the liver and kidney functions of the body.

According to the corn fermented feed and the preparation method thereof, yak rumen microorganisms and exogenous beneficial microorganisms which do not have antagonism with the yak rumen microorganisms are adopted for fermenting silage corn at the same time, and the corn fermented feed is obtained; wherein the exogenous beneficial microorganisms comprise at least one of lactobacillus plantarum, pediococcus pentosaceus, trichoderma koningii and lactobacillus buchneri. The method comprises the following steps: S1, collecting rumen fluid suitable for high-concentrate barn feeding daily ration yaks to obtain yak rumen microorganisms; s2, mixing yak rumen microorganisms and the activated exogenous beneficial microorganisms in sterile water to obtain a compound microorganism bacterium solution; s3, silage corn is inoculated with the compound microorganism bacterial liquid prepared in the step S2 for fermentation, and the corn fermented feed is obtained; wherein the fermentation temperature ranges from 25 DEG C to 32 DEG C, the fermentation pH value ranges from 3.8 to 4.5, and the fermentation time ranges from 36 h to 60 h.

The present application relates to a kind of composite methane inhibitor, slow-release methane inhibition microparticle and its preparation method and application, the active ingredient of the composite methane inhibitor includes monoglyceride lauric acid and composite additive, wherein, the composite additive includes at least two of malic acid, fumaric acid, tea saponin;The present application first combines monoglyceride lauric acid, malic acid and fumaric acid, tea saponin to prepare composite methane inhibitor, utilize the synergistic effect between each component to show excellent methane emission inhibition effect, and by multi-target regulation, effectively avoid the adaptive evolution of rumen microorganism to single inhibitor, and using safe, conventional raw material, production cost is low, with broad industrialization application prospect and research significance.

This invention relates to a composite methane inhibitor, sustained-release methane-inhibiting microparticles, their preparation method, and applications. The active ingredient of the composite methane inhibitor includes lauric acid monoglyceride and a composite additive, wherein the composite additive includes at least two of malic acid, fumaric acid, and tea saponin. This invention is the first to combine lauric acid monoglyceride, malic acid, fumaric acid, and tea saponin to prepare a composite methane inhibitor. It exhibits excellent methane emission inhibition by utilizing the synergistic effect between the components, and effectively avoids the adaptive evolution of rumen microorganisms to a single inhibitor through multi-target regulation. Furthermore, it uses safe and conventional raw materials, has low production costs, and possesses broad prospects for industrial application and research significance.

The invention relates to the technical field of resources and environments, in particular to a method for controlling rumen microorganisms to stably produce methane in vitro, which comprises the following steps: regulating and controlling a nitrogen-rich substrate to account for 50-75% of the total dry weight of a system, reducing ORP (oxidation-reduction potential) to below 350mV, and enriching hydrolytic acidification bacteria Clostridium and methanogenic bacteria Methanosarcina; the pH value is adjusted to 6.9 to 7.5, and the glycolysis and Enter-Doudoroff pathway flux is improved; by taking propionic acid metabolism delta Gpropanoate less than or equal to 42 kJ / mol and butyric acid metabolism delta Gbutanoate less than or equal to 90 kJ / mol as a stable judgment standard, regulating and controlling CO2 partial pressure and enhancing the expression of an acetic acid nutritional type methanogen gene mtaABC and a hydrogen nutritional type methanogen gene mtrH, wherein the acetic acid metabolism delta Gpropanoate is less than or equal to 42 kJ / mol and the butyric acid metabolism delta Gbutanoate is less than or equal to 90 kJ / mol; for the condition that the indexes do not reach the standard, optimization is carried out by increasing the proportion of nitrogen-rich substrates and regulating and controlling the hydrogen partial pressure to be 24.41 kPa or below. According to the method disclosed by the invention, the methane yield is stably improved and the activity of hemicellulase and cellulase is remarkably enhanced by regulating and controlling microbial communities, metabolic pathways and functional genes, so that the problem of methane yield fluctuation caused by improper in-vitro domestication is effectively solved, and a reliable technical scheme is provided for efficiently converting cellulose biomass into biological methane.

The invention discloses a feed for improving rumen microbial community structure and testis reproductive protein expression of black cattle in Bohai Sea and application, and relates to the technical field of animal nutrition and veterinary medicine. The technical scheme of the invention discloses a new application of vitamin B6 in improving rumen microbial community structure of black cattle in Bohai Sea and improving testis reproductive protein expression of the black cattle in Bohai Sea, the vitamin B6 is added into basic ration of the black cattle in Bohai Sea, and the black cattle are continuously fed for a period of time, so that the rumen microbial community structure can be obviously improved; the abundance of beneficial microorganisms is increased, rumen metabolites are adjusted, and the production performance of the black cattle in the Bohai Sea is enhanced. According to the technical scheme, the invention also discloses vitamin B6 which enhances the reproductive performance of black cattle in Bohai Sea by improving the expression of key proteins SYCP1, MVH and CYP11A1 in testis. A new thought is provided for feeding management of the black cattle in the Bohai sea, and the method has important application value and market prospect.