53 results about "Rumen microorganisms" patented technology

The invention discloses difunctional glucanase for hydrolyzing agricultural and sideline products and application, and belongs to the technical field of biology. The difunctional glucanase is named as RuCel071, and the amino acid sequence of the difunctional glucanase is as shown in SEQ ID NO. 3. The novel cellulase RuCe071 is developed on the basis of a rumen microorganism metagenome resource mining technology, has the activity of simultaneously hydrolyzing cellulose and xylan, can be used for degrading agricultural and sideline products, reducing accumulation of the agricultural and sideline products and environmental pollution, lowering the treatment cost and improving the degradation efficiency, and has the advantages of being simple in preparation process, low in cost and high in application value. The utilization rate and the economic benefit of agricultural and sideline products are favorably improved.

The invention discloses a multifunctional cellulolytic enzyme and application thereof in degradation of agricultural by-products, and belongs to the technical field of biology. The multifunctional cellulolytic enzyme is named RuCelI, and the amino acid sequence of the multifunctional cellulolytic enzyme is as shown in SEQ ID NO. 1. Based on a rumen microbial metagenome resource mining technology, a novel multifunctional cellulose decomposition enzyme RuCelI is developed, and the enzyme has double activities of endo-cellulase and xylanase, can be used for degrading agricultural by-products, reducing the treatment cost and improving the degradation efficiency, and is beneficial to improving the utilization rate and the economic benefit of the agricultural by-products.

The invention discloses methanogen lyase homologous with a PeiR lyase sequence and application of the methanogen lyase, and aims to solve the problem that PeiR lyase protein of a methanogen cell wall peptide bond can participate in a biological process of hydrolyzing archaea cell walls, effectively kill methanogen and reduce methane production, and protein homologous with the PeiR lyase protein has potential methane reduction potential. According to the invention, a series of biological information software is utilized to identify protein sequences coded by methanogens virus from rumen microbiome sequencing data, and sequence homology between the proteins and PeiR protein is further analyzed to obtain a series of methanogens lyases. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through the detection of crude enzyme liquid, the effectiveness of the lyase in the aspect of reducing the methane yield is verified in an in-vitro gas production test.

The invention discloses methanogen lyase homologous with high-dimensional characteristics of PeiW lyase and application of the methanogen lyase, and aims to solve the problem that PeiW lyase protein of methanogen cell wall peptide bonds can participate in the biological process of hydrolyzing archaea cell walls, effectively kill methanogen and reduce methane production, and protein homologous with the protein has potential methane reduction potential. According to the method, a series of bioinformatics software is utilized, a protein sequence coded by methanogens virus is identified from a large amount of rumen microorganism virus group sequencing data, the high-dimensional homology of a deep learning model between the protein sequence and PeiW is analyzed, and two methanogens lyases similar to the high-dimensional characteristics obtained by the PeiW model are obtained. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through detection of a crude enzyme solution, the effectiveness of the lyase in the aspect of reducing the yield of methane is verified in an in-vitro gas production test.

The invention discloses methanogen lyase homologous with high-dimensional characteristics of PeiR lyase and application of the methanogen lyase and aims to solve the problems that PeiR lyase protein of methanogen cell wall peptide bonds can participate in the biological process of hydrolyzing archaea cell walls, methanogen is effectively killed, methane production is reduced, and protein homologous with the protein has potential methane reduction potential. According to the invention, a series of biological information software is utilized to identify protein sequences coded by methanogen viruses from a large amount of rumen microbiome sequencing data, and high-dimensional homology between the proteins and PeiR proteins based on a deep learning model TM-Vec is further analyzed, so that a series of methanogen lyases are obtained. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through the detection of crude enzyme liquid, the effectiveness of the lyase in the aspect of reducing the methane yield is verified in an in-vitro gas production test.

The invention relates to the technical field of feed additives, and discloses a ruminant animal fattening and growth-promoting feed additive which comprises the following components in percentage by mass: 22-28 parts of phytosterol, 42-48 parts of a rumen protection type amino acid compound, 11-14 parts of a compound probiotic preparation, 6-7.5 parts of a compound enzyme preparation, 9-11 parts of a trace element compound and 4-4.8 parts of a buffer system. According to the preparation method of the fattening and growth-promoting feed additive for ruminant animals, a modified hydrogenated palm oil and lecithin compound is used as a rumen protection type amino acid coating material, and a high-pressure homogenizer is used for performing multi-stage homogenizing treatment, so that a coating layer is more compact in structure, and the weight of the coating layer is increased; the rumen microorganism lipase decomposition resistance is enhanced through the synergistic effect of the composite material, particle distribution is optimized in combination with a refined homogenizing process, the release controllability and absorption efficiency of amino acid in small intestines are improved, and the problems that a traditional single coating material is easy to decompose and the particle size is extensively controlled are solved.

The invention discloses cellulase and application thereof, and belongs to the technical field of protein engineering. The invention develops a novel cellulase (named RuCel066) based on a rumen microbial metagenome resource mining technology, and the cellulase has the capability of effectively hydrolyzing agricultural by-products. Research and application of the enzyme are helpful for improving utilization rate and economic benefit of agricultural by-products.

The invention relates to the technical field of resources and environment, in particular to a method for pretreating lignocellulose by using a rumen microorganism dry method. The method comprises the following steps: crushing lignocellulose to 1-5cm, and filling the crushed lignocellulose into a sealed space; spraying microorganisms under an anaerobic condition according to a mass ratio of rumen microorganisms to lignocellulose volatile components of (0.015-0.03): 1, and controlling the water content of the system to be 30-60%; the pretreatment temperature is regulated and controlled to be 35-42 DEG C, the pH is 6.0-7.5, the pretreatment time is 10-14 days, so that the total abundance of the functional microorganisms Pseudocidovorax, Hallella and Prevotella is greater than or equal to 55%, and the three types of microorganisms respectively form strong interaction with specific companion bacteria; when the relative crystallinity of the lignocellulose is reduced by more than 15% and the volatile component is reduced by 10%, finishing the pretreatment; and performing process regulation and control according to the crystallinity and the reducing sugar yield when the pretreatment is performed for 10 days. The dry process is adopted, the bionic principle and the solid-state fermentation technology are combined, all-component gradient utilization of lignocellulose is achieved, a green solution is provided for industrial and high-value conversion of lignocellulose, and remarkable economic, social and environment-friendly benefits are achieved.

The invention discloses a method for predicting the sperm motility of a breeding bull based on a host SNP-rumen microorganism-metabolite interaction axis, and the sperm motility of the breeding bull can be obtained by detecting the haplotype condition of SNP1-SNP3 and / or SNP4-SNP6 sites of a breeding bull genome. The method breaks through the limitations of strong dependency on phenotype endpoint evaluation and prediction lag in the current bull seed selection process, and realizes forward accurate prediction and early evaluation of the semen quality of the breeding bull.

This invention discloses a methanogenic lyase homologous to PeiR lyase and its applications. The PeiR lyase protein, targeting the peptide bonds of the methanogenic cell wall, participates in the biological process of hydrolyzing the archaea cell wall, effectively killing methanogens and reducing methane production. Proteins homologous to this lyase possess potential methane-reducing potential. This invention utilizes a series of bioinformatics software to identify protein sequences encoded by methanogenic viruses from a large amount of rumen microbial virome sequencing data, and analyzes the structural homology between these proteins and PeiR. Ultimately, this invention successfully obtained a series of methanogenic lyases homologous to PeiR. To achieve the expression of these lyases, this invention designed multiple primers to synthesize the target sequences and successfully expressed them in a prokaryotic expression system. In vitro gas production experiments with the crude enzyme solution showed that these lyases are effective in reducing methane production.

The invention provides a rumen bypass folic acid additive, a preparation method and application thereof and a method for producing milk rich in folic acid, and particularly belongs to the technical field of rumen bypass additives. The rumen bypass folic acid additive comprises folic acid, cobalamin, silicon dioxide, lipase, palm fat, coconut oil and calcium stearate. The rumen bypass folic acid additive can reduce folic acid degradation by rumen microorganisms, can directly reach small intestines to be absorbed, improves the folic acid utilization rate of ruminants, specifically can improve the milk yield, milk protein, milk fat and folic acid content and yield of dairy cows, and can produce folic acid-rich cow milk; the feed can also increase the plasma glucose level and reduce the concentration of beta-hydroxybutyric acid, so that the energy metabolism state of the dairy cow is improved, the production performance of the dairy cow is improved, and economic benefits are brought to pastures.

The invention discloses a novel GAA enzyme for improving the degradability of lignocellulose and application of the novel GAA enzyme, the GAA enzyme is derived from 1, 4-benzoquinone reductase of an AA6 family of ruminant rumen microorganisms, and the amino acid sequence of the GAA enzyme is shown as SEQ ID NO.1. Experiments prove that the enzyme has high catalytic activity and stability in a rumen environment (pH 6.0-6.8, 39 DEG C), can effectively improve the degradation rate of lignocellulose, and has wide application prospects in preparation of enzyme preparations, feed additives or microecologics for improving the utilization rate of lignocellulose in ruminant roughage.

The invention discloses methanogen lyase homologous with high-dimensional characteristics of PeiP lyase and application of the methanogen lyase, and aims to solve the problem that PeiP lyase protein of methanogen cell wall peptide bonds can participate in the biological process of hydrolyzing archaea cell walls, effectively kill methanogen and reduce methane production, and protein homologous with the protein has potential methane reduction potential. According to the method, a series of bioinformatics software is utilized, a protein sequence coded by methanogens virus is identified from a large amount of rumen microorganism virus group sequencing data, the high-dimensional homology of a deep learning model TM-Vec between the protein sequence and PeiP is analyzed, and the methanogens lyase homologous with the PeiP on the high-dimensional feature level of the model is obtained. According to the invention, multiple segments of primers are designed to synthesize a target sequence, the expression of the lyase is successfully realized in a prokaryotic expression system, and the effectiveness of the novel lyase in the aspect of reducing the yield of methane is verified in an in-vitro gas production test by detecting a crude enzyme solution.

The invention discloses methanogen lyase homologous with a PeiW lyase structure and application of the methanogen lyase homologous with the PeiW lyase structure, and the methanogen lyase is obtained by screening from rumen microbiome sequencing data through a structure comparison method based on known PeiW lyase characteristics aiming at methanogen cell wall peptide bonds. The PeiW lyase protein can participate in the biological process of hydrolyzing the cell wall of methanogens, the methanogens are effectively killed, methane generation is reduced, and the protein homologous with the protein structure has potential methane reduction potential. According to the invention, rumen metagenome data and virome data are integrated, potential protease is screened by using bioinformatics software, and a sequence homologous with a PeiW structure is identified to obtain two novel lyases. A target sequence is synthesized through multiple segments of primers and is expressed in a prokaryotic expression system, and meanwhile, a crude enzyme liquid in-vitro gas production test shows that the lyase can remarkably reduce the methane yield.

The invention discloses a bezoar quality improving method based on natural pasture environment optimization. According to the method, the internal quality of the calculus bovis is remarkably improved through multi-dimensional cooperative regulation and control. A natural feed basis with balanced nutrition is provided for cattle from soil environment baseline investigation to precise pasture proportioning, and by combining directional cultivation and implantation of genetic engineering strains, the metabolic activity of rumen microorganisms is effectively enhanced, and accumulation of key components for synthesis of calculus bovis is promoted. The linkage application of the intelligent monitoring system and the environment regulation and control device optimizes the living environment of cattle in real time, and cooperates with innovative intervention means such as lavender essential oil and music therapy, so that the stress reaction of cattle herds is remarkably reduced, and the efficient operation of liver metabolic pathways is guaranteed. The directional feeding of the functional lick bricks further enhances the conversion efficiency of the calculus bovis precursor substance, the whole-process data tracing ensures the controllability of the quality formation process, and finally the improvement of the content of effective active ingredients in the calculus bovis and the enhancement of the quality stability are realized.

The invention belongs to the technical field of livestock feed, and particularly discloses a total mixed ration containing fermented cotton straw and application thereof. The total mixed ration comprises the following raw materials on the basis of dry matter: 5-15% of fermented cotton straw, 10-20% of corn straw, 5-10% of alfalfa, 25% of corn, 9% of soybean meal, 7.5% of dry corn vinasse, 10% of corn germ meal, 15% of soybean hull, 0.9% of calcium hydrophosphate, 0.4% of ammonium chloride, 0.2% of choline chloride, 0.5% of sodium chloride and 0.5% of sodium bicarbonate. The total mixed ration can significantly improve the growth performance and feed conversion efficiency of the Hu sheep, improve the digestion and utilization rate of nutrient substances, effectively improve the health and anti-stress level of animal bodies, improve the diversity and structure of rumen microflora, promote the growth of beneficial microorganisms, and establish a more stable and efficient rumen fermentation system.

The application discloses a multifunctional fiber-degrading enzyme and application thereof, and belongs to the technical field of biology. The multifunctional fiber-degrading enzyme is named RuCelF, and the amino acid sequence is shown as SEQ ID NO. 1. Based on rumen microbial metagenomic resource mining technology, a new multifunctional fiber-degrading enzyme RuCelF is developed. The enzyme has double activities of cellulase and xylanase, can be used for degrading agricultural waste, reduces treatment cost, improves degradation efficiency, helps to improve the utilization rate and economic benefits of agricultural waste, and provides a new enzyme source and technical support for efficient resource utilization of agricultural waste.

The invention relates to the technical field of animal husbandry, and discloses application of hemicellulose and mannan in regulation of growth performance of sheep. The content of hemicellulose is 10.3% DM or 17% DM, and the addition amount of mannan is 80-160 mg / kg DM. According to the application of the hemicellulose and the mannan in regulating the growth performance of the sheep, the mannan is added into daily ration, so that the digestibility of animals to dry matters, crude proteins, neutral detergent fibers and acid detergent fibers can be increased, and the content of total acid, acetic acid, propionic acid, butyric acid and other volatile fatty acids in rumen can be increased; according to the present invention, with the feed, the richness and the diversity of the flora are increased, the richness of the firmicophyta, the spirochete and the like is improved, the intestinal flora of the sheep is adjusted, the blood sugar is reduced, and the daily ration with different hemicellulose levels has different influences on the growth performance, the rumen microorganisms, the metabolite and the lipid metabolism of the sheep; and a theoretical basis is provided for research and development of the yeast-source functional feed additive.

The present application relates to the field of cosmetic technology, and discloses a preparation process of cashmere goat bone-derived elastin and application thereof in cosmetics. The process comprises: defatting cashmere goat bone particles; using composite rumen microbial flora to perform bionic fermentation to destruct bone matrix and expose active sites; adopting neutral protease and specific elastase to perform stepwise directional enzymolysis; performing hydrophilic modification through transglutaminase catalysis of covalent anchoring of polyglutamic acid and elastin polypeptide; and finally, performing multi-stage membrane interception purification and drying. The present application realizes complete retention of the natural cross-linking structure of elastin under mild conditions, significantly improves water solubility, biological activity and transdermal permeability of the elastin, solves the problems of structural damage and pollution caused by traditional chemical methods, and provides a green path for high-value utilization of cashmere goat by-products.

The invention discloses methanogen lyase homologous with a PeiP lyase structure and application of the methanogen lyase, and the methanogen lyase is screened from rumen microbiome sequencing data through a structure comparison method based on known PeiP lyase protein characteristics. The PeiP lyase protein can participate in the biological process of hydrolyzing archaea cell walls, methanogens are effectively killed, methane production is reduced, and the protein homologous with the protein has potential methane reduction potential. Protein sequences coded by methanogens viruses are extracted from rumen microbiome sequencing data, the structural similarity between the proteins and PeiP proteins is analyzed by using a bioinformatics tool, and finally two lyases are successfully identified. In order to realize the expression, multiple segments of primers are designed and synthesized to construct a target sequence, and high-efficiency expression is realized in a prokaryotic expression system. In-vitro gas production experiments show that the crude enzyme liquid can significantly reduce methane production.

The invention discloses a target editing system of a methanosarcina hdrE gene, an in-vitro evaluation method of influence of the methanosarcina hdrE gene on bovine rumen methane generation and application, and relates to the technical field of gene editing, and the method comprises the following steps: constructing the target editing system of the methanosarcina hdrE gene; mixing the target editing system of the methane sarcina hdrE gene with the bovine rumen fluid, the silage and the conversion mixed solution to form an in-vitro fermentation system; culturing a fermentation system under the anaerobic condition of 39 DEG C; detecting at least one of the following indexes: methane sarcina relative abundance, methane gas generation amount and rumen microflora structure change; according to the method, the influence of the target editing system of the methanosarcina hdrE gene on bovine rumen methane generation is comprehensively and deeply evaluated from multiple dimensions such as the gene editing effect, the number change of target microorganisms and the whole rumen microorganism ecosystem.

The invention relates to the technical field of livestock breeding, and discloses a preparation method of a slow-release acid-control premix for cattle and sheep feed production, the premix is prepared by fully mixing a plurality of micronutrients with a carrier according to a specific proportion, and the premix comprises the following components: vitamins such as vitamin A, vitamin D, vitamin E, vitamin B and the like; for example, iron, copper, zinc, manganese, selenium, iodine and the like; an enzyme preparation, such as cellulase, amylase, phytase; probiotics and saccharomycetes; buffer agents: calcium formate and magnesium oxide; an antioxidant, such as ethoxyquin, BHT; and a mildew inhibitor such as potassium acid calcium. According to the preparation method of the slow-release acid-control premix produced by the cattle and sheep feed, through the acid-control additive, a suitable environment for rumen microorganisms is maintained, nutrient absorption is promoted, acidosis is prevented, subclinical or acute rumen acidosis caused by too high acid load is prevented, and the utilization rate of the feed is improved; the feed conversion rate is improved.

The present invention discloses a targeted editing system of the hdrE gene of Methanosarcina and an in vitro evaluation method and application of its effect on bovine rumen methane production, relating to the field of gene editing technology, comprising the following steps: constructing a targeted editing system of the hdrE gene of Methanosarcina; mixing the targeted editing system of the hdrE gene of Methanosarcina with bovine rumen fluid, silage, and conversion mixture to form an in vitro fermentation system; culturing the fermentation system under anaerobic conditions at 39°C; and detecting at least one of the following indicators: relative abundance of Methanosarcina, methane gas production, and changes in rumen microbial community structure. The present invention comprehensively and deeply evaluates the effect of the targeted editing system of the hdrE gene of Methanosarcina on bovine rumen methane production from multiple dimensions, including gene editing effect, changes in the number of target microorganisms, and the entire rumen microbial ecosystem.

The invention discloses xylanase and application thereof, and belongs to the technical field of biology. The xylanase is named as RuXynJ, and the amino acid sequence of the xylanase is as shown in SEQ ID NO. 1. The novel xylanase RuXynJ is developed on the basis of a rumen microbial metagenome resource mining technology, has xylanase activity, can be used for degrading agricultural wastes, reducing the treatment cost and improving the degradation efficiency, is beneficial to improving the utilization rate and economic benefits of the agricultural wastes, and has a wide application prospect. And a basic support is provided for efficient resource utilization of agricultural wastes.

The invention discloses methanogen lyase homologous with a PeiR lyase structure and application of the methanogen lyase and aims to solve the problems that PeiR lyase protein of methanogen cell wall peptide bonds can participate in the biological process of hydrolyzing archaea cell walls, methanogen is effectively killed, methane production is reduced, and protein homologous with the protein has potential methane reduction potential. According to the invention, a series of bioinformatics software is utilized to identify the protein sequence coded by methanogens virus from a large amount of rumen microorganism virus group sequencing data, and the structural homology between the protein and PeiR is analyzed on the basis. Finally, the invention successfully obtains a series of methanogen lyase homologous with the PeiR structure. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. An in-vitro gas production test of a crude enzyme solution shows that the lyase is effective in the aspect of reducing the yield of methane.

The invention discloses a lactating cow feed prepared by combining plant extracts and probiotics and a preparation method of the lactating cow feed. The feed consists of the following components: whole-plant corn silage, alfalfa hay, oat hay, steam-flaked corn, corn flour, whole cottonseeds, beet pulp, soybean meal, rumen-protected soybean meal, puffed soybean meal, double-low rapeseed meal, a premix, fat powder, baking soda, a plant extract and probiotics. Plant active ingredients such as glycine betaine, astragalus polysaccharide and allicin are integrated and combined with bacillus subtilis, bacillus licheniformis and lactic acid bacteria to construct a multi-target regulation and control system, and glycine betaine can improve milk yield and milk quality and improve energy metabolism; allicin can reduce emission of ammonia nitrogen and methane; the digestibility of nutrient substances can be improved by the bacillus subtilis and the bacillus licheniformis; the lactic acid bacteria can optimize the microbial community structure. Through the synergistic effect of multiple components, the rumen microbial protein synthesis efficiency is improved, and a scientific basis is provided for improving nitrogen conversion of dairy cow feed.

The invention discloses a difunctional expansin protein mutant as well as a coding gene and application thereof, and belongs to the technical field of biological genetic engineering. According to the invention, a protein PFLoos1 with bifunctional activity is obtained from a metagenome of rumen microorganisms, and mutants PFLoos1-S1, PFLoos1-S2 and PFLoos1-S3 are obtained by carrying out site-specific mutagenesis on key amino acids in a substrate binding pocket or a surface hydrophobic region of the protein PFLoos1. The mutant provided by the invention has the function of inhibiting the metabolic activity of rumen methanogenic archaea on the basis of keeping the lignocellulose degradation promoting capability. The mutant provided by the invention not only can be applied to preparation of an enzyme preparation for promoting cellulose degradation, but also can be applied to a feed additive for inhibiting ruminant rumen methane generation, and has important practical value for improving feed energy utilization efficiency and low-carbon breeding of animal husbandry.

The application relates to a microbial preparation passing through the rumen, a preparation method and application thereof. The microbial preparation passing through the rumen comprises microbial powder and a coating material coated on the surface of the microbial powder. The coating material comprises protective fat. The total weight of fatty acids in the microbial preparation passing through the rumen is 100%, the fatty acids comprise 70wt%-80wt% of palmitic acid and stearic acid, <=9wt% of oleic acid, 5wt%-15wt% of polyunsaturated fatty acids and a residual amount of non-target fatty acids, the mass ratio of the palmitic acid to the stearic acid is 1:1-2:1, the polyunsaturated fatty acids are selected from at least one of docosahexaenoic acid, eicosatetraenoic acid and eicosapentaenoic acid, and the mass ratio of the microbial powder to the protective fat is 2:3-3:2. The microbial preparation passing through the rumen described in the application is used as a feed additive for feeding ruminants such as cows, and can realize the dual improvement of the production performance of the cows and the nutrition strengthening of the milk.

The application provides application of a grifola ferroxida ferment in preparation of a feed additive for reducing methane emission of a dairy cow. The grifola ferroxida ferment is obtained by liquid submerged fermentation or liquid-solid two-phase fermentation with grifola ferroxida as an object. After the grifola ferroxida ferment is fed to the dairy cow, the activity of a fiber-decomposing bacterial group can be enhanced, the feed digestion rate is improved, the abundance of some bacteria in a rumen is improved, the activity composition of a rumen microbial population is changed, and the methane emission of the dairy cow is reduced.

The present invention discloses a bifunctional glucanase for hydrolyzing agricultural and sideline products and its application, belonging to the field of biotechnology. The bifunctional glucanase is named RuCel071, and its amino acid sequence is shown in SEQ ID NO. 3. Based on rumen microbial metagenomic resource mining technology, the present invention develops a cellulase RuCel071, which has the activity of simultaneously hydrolyzing cellulose and xylan. This enzyme can be used to degrade agricultural and sideline products, reduce agricultural and sideline product accumulation and environmental pollution, lower processing costs, improve degradation efficiency, and help improve the utilization rate and economic benefits of agricultural and sideline products.