Preparation process of bone-derived elastin of cashmere goats and application thereof in cosmetics

Abstract

The present application relates to the field of cosmetic technology, and discloses a preparation process of cashmere goat bone-derived elastin and application thereof in cosmetics. The process comprises: defatting cashmere goat bone particles; using composite rumen microbial flora to perform bionic fermentation to destruct bone matrix and expose active sites; adopting neutral protease and specific elastase to perform stepwise directional enzymolysis; performing hydrophilic modification through transglutaminase catalysis of covalent anchoring of polyglutamic acid and elastin polypeptide; and finally, performing multi-stage membrane interception purification and drying. The present application realizes complete retention of the natural cross-linking structure of elastin under mild conditions, significantly improves water solubility, biological activity and transdermal permeability of the elastin, solves the problems of structural damage and pollution caused by traditional chemical methods, and provides a green path for high-value utilization of cashmere goat by-products.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to a preparation process of cashmere goat bone-derived elastin and its application in cosmetics. Background Technology

[0002] Elastin, as a core component of the extracellular matrix, has become a research hotspot in the fields of anti-aging and tissue repair due to its unique elastic modulus, excellent biocompatibility, and bioactivity inducing fibroblast proliferation. Cashmere goats, a unique biological resource in my country, possess bone tissue that is not only rich in minerals but also contains high-quality bone-derived elastin.

[0003] In existing industrial practices, the separation of elastin from highly mineralized bone tissue usually requires the use of high-concentration inorganic acids for long-term decalcification in order to completely remove hydroxyapatite from the bone matrix and remove collagen and non-collagenous proteins that are tightly bound to it, supplemented by oxidation or reduction hydrolysis under high temperature conditions in a strong alkaline environment.

[0004] The core function of elastin lies in its unique intramolecular and intermolecular cross-linking structure, namely the three-dimensional network system composed of desmokinin and isodesmokinin. This is the physicochemical basis for maintaining its natural elasticity, triple helix conformation, and mechanical stability. Strong acids, strong alkalis, and high temperatures often exhibit non-specific degradation characteristics. While dissolving the bone matrix, they inevitably induce disordered breakage of polypeptide chains and chemical modification of key cross-linking bonds. This results in a severely damaged microstructure in the final protein product, a significant loss of biological activity, and a substantial degradation of mechanical elasticity, making it difficult to achieve the expected skin care effects.

[0005] Chemical residues can also affect the safety evaluation of bone-derived proteins, as even trace amounts of solvent residue can irritate sensitive skin. Furthermore, elastin prepared using traditional processes often exhibits defects such as poor hydrophilicity, low transdermal penetration efficiency, and poor compatibility. Summary of the Invention

[0006] To address the technical problems of existing chemical extraction methods for cashmere goat bone-derived elastin, which result in molecular structural damage, loss of bioactivity, and severe environmental pollution, this invention provides a preparation process for cashmere goat bone-derived elastin and its application in cosmetics. This invention simulates the rumen fermentation environment of ruminants, coupling targeted stepwise enzymatic hydrolysis with microbial metabolic modification techniques to achieve efficient extraction and structural protection of elastin from the bone matrix under mild conditions, endowing the product with excellent hydrophilicity and biocompatibility.

[0007] To achieve the above-mentioned objective, this invention provides a preparation process for cashmere goat bone-derived elastin, characterized by comprising the following steps: The first step is raw material pretreatment. Cashmere goat bones, specifically the long bones of the limbs of cashmere goats aged 12-24 months, are selected. The attached muscles, connective tissue, and bone marrow on the bone surface are removed, and the bones are mechanically crushed into bone particles with a diameter of 0.5-1.0 cm. The bone particles are placed in a multi-frequency ultrasonic cleaner, and a degreasing solution is added. This degreasing solution is a mixture of ethanol and petroleum ether at a volume ratio of 1:2. The cleaning is performed for 2-4 hours at a frequency of 25-40 kHz and a temperature of 35-40°C to remove the fatty components from the bone matrix. The degreased bone particles are washed with deionized water until neutral and then vacuum dried at 45°C.

[0008] The second step is biomimetic rumen pretreatment. An anaerobic bioreactor simulating the rumen environment of a cashmere goat is constructed. The reactor is equipped with a double-layered stainless steel jacket for circulating constant-temperature water and features an online pH monitoring system and an oxidation-reduction potential sensor. Artificial rumen fluid is added to the reactor, the solutes of which include sodium bicarbonate, disodium hydrogen phosphate, sodium chloride, potassium chloride, magnesium sulfate, and calcium chloride. Pretreated bone particles are added to the reactor at a liquid-to-solid mass ratio of 10:1 to 15:1. A mixture of carbon dioxide and nitrogen is introduced into the reactor to maintain the anaerobic environment. A composite rumen microbial community is inoculated, consisting of succinate-producing filamentous bacilli, white rumenococci, and yellow rumenococci in a ratio of 3:2:2. Fermentation is carried out for 24-48 hours at a temperature of 38.5-39.5℃, a pH of 6.2-6.8, and a stirring speed of 40-60 rpm.

[0009] During the biomimetic rumen pretreatment process, the composite rumen microbial community secretes extracellular cellulase, hemicellulase, and non-specific proteases. Through chemotaxis, the microorganisms adhere to the surface of bone particles and their pores. Utilizing the synergistic effect of enzymes produced by cellulogenic bacteria, proteoglycans, mucopolysaccharides, and glycoproteins embedded between hydroxyapatite and elastin fibers in the bone matrix are selectively degraded. This process breaks down the physical barrier and chemical anchoring force between bone minerals and elastin, causing the tightly wrapped elastin fiber bundles to loosen and expose the active sites for subsequent enzymatic hydrolysis. Simultaneously, the metabolic environment of the rumen microorganisms maintains the chemical stability of the desmokine and isodesmokine cross-linking bridges within the elastin molecules, preventing oxidative breakage.

[0010] The third step is stepwise directional enzymatic hydrolysis. After fermentation, the reaction system is heated to 85°C and maintained for 15 minutes to inactivate rumen microorganisms. The temperature is then lowered to 50-55°C, and the pH is adjusted to 7.5-8.2. The first-stage enzyme preparation, a neutral protease with an activity of 5000-8000 U / g, is added, and the reaction time is 4-6 hours, to further remove residual collagen and other proteins from the bone matrix. Subsequently, the pH is adjusted to 8.5-9.0, and the second-stage enzyme preparation, a specific elastase, is added. This specific elastase is purified by deep fermentation of actinomycetes and exhibits high substrate specificity for elastin. The addition amount is 1%-3% of the bone particle mass. Enzymatic hydrolysis continues at 55°C for 8-12 hours, allowing elastin to be completely released from the bone matrix and hydrolyzed into polypeptide fragments with specific molecular weight distributions.

[0011] Step four: hydrophilic modification and metabolic anchoring. Five hours into the second stage of enzymatic hydrolysis, modified bacterial metabolites and transglutaminase are added to the reaction system. The modified bacterial metabolites contain a high concentration of polyglutamic acid. The amount of transglutaminase added is 30-50 U / g. Under the catalysis of transglutaminase, the polyglutamic acid chain segment with multiple carboxyl groups is covalently anchored to the lysine residues of the elastin polypeptide side chain. This step achieves in-situ hydrophilic modification of the elastin molecule, significantly improving its solubility and osmotic stability in the aqueous system.

[0012] Step 5: Molecular interception and purification. The enzymatic hydrolysate is centrifuged at 8000-10000 rpm for 20 minutes, and the supernatant is collected. A continuous cross-flow filtration system is used for multi-stage extraction. First, fine bone fragments and bacterial debris are removed through a 0.45 μm microfiltration membrane; then, large, undegraded proteins are removed through a 50 kDa ultrafiltration membrane; finally, desalting and concentration are achieved through a 3-10 kDa nanofiltration membrane. The resulting concentrate is further purified by electrodialysis to remove inorganic salt ions, reducing the conductivity to below 50 μS / cm.

[0013] Step 6: Finished Product Drying. The purified cashmere goat bone-derived elastin concentrate is spray-dried or freeze-dried under vacuum. If spray drying is used, the inlet air temperature is set to 150-160℃, the outlet air temperature to 75-80℃, and the centrifugal atomizer speed to 18000 rpm. If freeze-drying is used, the product is pre-frozen at -45℃ for 4 hours, followed by sublimation under a vacuum of less than 10 Pa, with the final product moisture content controlled below 3%.

[0014] This invention also provides the application of cashmere goat bone-derived elastin in cosmetics. The cashmere goat bone-derived elastin is used as a core active ingredient, and its addition amount is 0.1%-5.0% of the total mass of the cosmetic.

[0015] In a preferred embodiment of the present invention, in cosmetic applications, the cashmere goat bone-derived elastin, low-molecular-weight sodium hyaluronate, and ceramide constitute a composite modification system. The low-molecular-weight sodium hyaluronate has a molecular weight of 3-5 kDa, and its mass ratio to elastin is 1:5. The ceramide is embedded in polypeptide micelles formed by elastin using a high-pressure homogenization process, forming a stable transdermal delivery system.

[0016] In cosmetic formulations, the cashmere goat bone-derived elastin not only provides natural elastic support and improves skin elasticity by replenishing the extracellular matrix of the dermis, but also utilizes its side-chain-loaded polyglutamic acid hydrophilic groups to form a breathable, semi-permeable biomembrane on the skin surface. This membrane effectively locks in moisture and utilizes the unique cross-linking structure of elastin to produce a physical firming and lifting effect.

[0017] In a preferred embodiment of the present invention, in the biomimetic rumen pretreatment step of the preparation process, the pH value of the artificial rumen fluid is maintained constant by an automatic titration system. The acidity regulator used is lactic acid, and the alkalinity regulator is sodium carbonate solution. This precise pH gradient control ensures that the metabolic activity of the fibrogenic microbiota is at its peak, achieving precise dissection of the bone matrix microstructure.

[0018] In the aforementioned stepwise directional enzymatic hydrolysis process, the molecular weight distribution of the product can be precisely controlled by adjusting the hydrolysis time of elastase. When the hydrolysis time is 8 hours, the molecular weight of the product is concentrated in the range of 10-30 kDa, which is suitable for firming cosmetics; when the hydrolysis time is extended to 12 hours, the molecular weight of the product is concentrated in the range of 3-5 kDa, which is suitable for deep repair cosmetics.

[0019] Compared with the prior art, the beneficial effects of the present invention are: 1. By replacing the traditional strong acid and strong alkali decalcification process with biomimetic rumen fermentation technology, the bone matrix is ​​deconstructed under a mild biochemical environment by utilizing the specificity of microbial enzyme systems. This process avoids the damage to the desmokine and isodesmokine cross-links within the elastin molecule caused by extreme pH values, ensuring that the prepared elastin has a triple helix conformation and mechanical resilience highly consistent with natural tissue, and its bioactivity is improved compared to traditional processes.

[0020] 2. The entire process is carried out in a near-neutral aqueous environment, without involving high concentrations of inorganic acids, strong alkalis, or harmful organic solvents. Both the biomimetic rumen fluid and enzymatic hydrolysis products exhibit excellent biodegradability, significantly reducing the pressure and cost of wastewater treatment and fully complying with green manufacturing and sustainable development industrial standards. Simultaneously, it avoids the risk of skin irritation from residual chemical solvents, enhancing the safety of the product in cosmetic applications.

[0021] 3. By employing microbial metabolic coupling technology, covalent anchoring of hydrophilic groups is simultaneously achieved during the extraction process. This solves the technical challenges of natural elastin's strong hydrophobicity, poor water solubility, and difficulty in formulation. The modified cashmere goat bone-derived elastin exhibits significantly improved solubility in water, achieving complete solubility, and remains stable even in electrolyte environments commonly found in cosmetic formulations, without precipitation or sedimentation.

[0022] 4. Specific molecular weight fragments obtained through stepwise directional enzymatic hydrolysis, combined with the surface activity generated by hydrophilic modification, enable elastin peptides to penetrate the stratum corneum barrier more efficiently and enter the dermis to exert their bio-inducing effects. The elastin prepared by this invention can significantly promote the proliferation of fibroblasts and the synthesis of endogenous collagen, exhibiting excellent anti-wrinkle and barrier repair effects. Detailed Implementation

[0023] This invention provides a preparation process for cashmere goat bone-derived elastin and its application in cosmetics. By constructing a biomimetic rumen environment and utilizing the synergistic metabolic effects of specific microbial communities, the microstructure of the bone matrix is ​​precisely deconstructed under mild conditions. Subsequently, a step-by-step directional enzymatic hydrolysis and in-situ hydrophilic modification technique are coupled to prepare a cashmere goat bone-derived elastin with intact structure, high solubility, and excellent skin compatibility.

[0024] The technical solution of the present invention will be described in detail below with reference to specific embodiments and comparative examples, so as to ensure that those skilled in the art can fully understand and implement the present invention.

[0025] Example 1: Long bones of the limbs of an 18-month-old cashmere goat were mechanically crushed into 0.8cm bone particles; The degreasing solution is made by mixing anhydrous ethanol and petroleum ether in a volume ratio of 1:2; The complex rumen microbiota consists of succinic acid-producing filamentous bacilli, white rumen cocci, and yellow rumen cocci in a ratio of 3:2:2. The artificial rumen fluid contains 9.8 g / L sodium bicarbonate, 3.7 g / L disodium hydrogen phosphate, 0.47 g / L sodium chloride, 0.57 g / L potassium chloride, 0.12 g / L magnesium sulfate, and 0.04 g / L calcium chloride. The first-stage enzyme preparation is a neutral protease with an enzyme activity of 6500 U / g; The second-stage enzyme preparation is a specific elastase, added at 2% of the bone particle mass. The modified strain metabolites contained 5% polyglutamic acid by mass. Transglutaminase dosage: 40 U / g; liquid-to-solid mass ratio: 12:1; The ratio of the number of rumen microbiota was 3:2:2, and the ratio of liquid to solid mass was 12:1.

[0026] Preparation steps: S1: Raw material pretreatment: Remove the attached muscle, connective tissue and bone marrow from the surface of cashmere goat bones, and mechanically crush them into 0.8cm bone particles; Place the bone particles in a multi-frequency ultrasonic cleaner, add degreasing solution to completely submerge the bone particles, and treat them for 3 hours at 38℃ with alternating operation at 25-40kHz; wash with deionized water until neutral, and vacuum dry at 45℃. S2: Bionic rumen pretreatment, constructing an anaerobic bioreactor, adding artificial rumen fluid, adding dried bone particles, maintaining a liquid-to-solid mass ratio of 12:1; introducing a mixture of carbon dioxide and nitrogen gas to maintain an anaerobic environment, inoculating with a composite rumen microbial community, fermenting for 36 hours at 39℃, pH 6.5, and stirring speed of 50 rpm, maintaining an oxidation-reduction potential of -220 mV, and adjusting the pH value with lactic acid and sodium carbonate solution using an automatic titration system; S3: Stepwise directional enzymatic hydrolysis. After fermentation, the system is heated to 85℃ and held for 15 minutes to inactivate microorganisms. The temperature is then lowered to 52℃, the pH is adjusted to 7.8, and neutral protease is added for 5 hours. Subsequently, the pH is adjusted to 8.8, specific elastase is added, and enzymatic hydrolysis is carried out at 55℃ for 10 hours. S4: Hydrophilic modification and metabolic anchoring. When the second stage of enzymatic hydrolysis reaches 5 hours, the modified strain metabolites and transglutaminase are added to the system, and the reaction continues until the end of enzymatic hydrolysis. S5: Molecular interception and purification. The enzymatic hydrolysate was centrifuged at 9000 rpm for 20 minutes. The supernatant was then passed through a 0.45 μm microfiltration membrane, a 50 kDa ultrafiltration membrane, and a 5 kDa nanofiltration membrane for graded filtration and concentration. The concentrate was desalted by electrodialysis to reduce the conductivity to 45 μS / cm. S6: Finished product drying. The purified concentrate is freeze-dried under vacuum. First, it is pre-frozen at -45℃ for 4 hours, and then sublimated and dried under a vacuum of less than 10Pa to obtain cashmere goat bone-derived elastin powder with a moisture content of 2.5%.

[0027] Example 2: Liquid-to-solid mass ratio 10:1, other components and proportions are the same as in Example 1; Preparation steps: Same as in Example 1.

[0028] Example 3: Liquid-to-solid mass ratio 15:1, other components and proportions are the same as in Example 1; Preparation steps: Same as in Example 1.

[0029] Example 4: The remaining components and proportions are the same as in Example 1; Preparation steps: The biomimetic rumen pretreatment fermentation time is 24 hours, and the remaining steps are the same as in Example 1.

[0030] Example 5: The remaining components and proportions are the same as in Example 1; Preparation steps: The biomimetic rumen pretreatment fermentation time is 48 hours, and the remaining steps are the same as in Example 1.

[0031] Example 6: The amount of specific elastase added was 1% of the bone particle mass, and the remaining components and proportions were the same as in Example 1; Preparation steps: Same as in Example 1.

[0032] Example 7: The amount of specific elastase added was 3% of the bone particle mass, and the remaining components and proportions were the same as in Example 1; Preparation steps: Same as in Example 1.

[0033] Example 8: Transglutaminase addition amount 50U / g, other components and proportions are the same as in Example 1; Preparation steps: Same as in Example 1.

[0034] Comparative Example 1: Same as Example 1; Preparation steps: The biomimetic rumen pretreatment step was eliminated, and the bone particles were directly subjected to stepwise directional enzymatic hydrolysis after defatting and drying. The remaining steps were adjusted accordingly.

[0035] Comparative Example 2: The specific elastase was removed, and only neutral protease was used for enzymatic hydrolysis. The other components were the same as in Example 1. Preparation steps: In the stepwise directional enzymatic hydrolysis stage, only neutral protease is added, and the hydrolysis time is extended to 16 hours. The remaining process parameters and steps are the same as in Example 1.

[0036] Test method: Elastin extraction rate test: The protein content in the finished product was determined by the Kjeldahl method, and the extraction rate was calculated by combining the theoretical elastin content in the raw material; Molecular weight distribution test: The molecular weight distribution range and peak molecular weight of elastin peptides were determined by gel permeation chromatography. Water solubility test: Prepare aqueous solutions of different concentrations of the finished product, observe their dissolution state, and measure the transmittance of the 1% concentration aqueous solution; Moisture content test: The moisture content of the finished product was determined using the Karl Fischer moisture determination method; Desmocin content test: The content of desmocin in the finished product was determined by high performance liquid chromatography to verify the retention of the natural cross-linking structure; Cell proliferation activity test: The MTT assay was used to determine the rate of cell proliferation promotion of the finished product on fibroblasts to verify its biological activity.

[0037] The test data comparisons are shown in Table 1 and Table 2.

[0038] Table 1. Comparison of Extraction Rate, Peak Molecular Weight, and Water-Soluble Transmittance ; Table 2 Comparison of desmokinin content, moisture content, and fibroblast proliferation rate ; Examples 1-8 utilize the core technology principle of biomimetic rumen fermentation and stepwise directional enzymatic hydrolysis to selectively degrade miscellaneous proteins and glycosaminoglycans in the bone matrix using a composite rumen microbial community. This loosens elastin fiber bundles and exposes active sites. Combined with neutral protease to remove residual miscellaneous proteins and specific elastase to release elastin peptides in a targeted manner, along with hydrophilic modification catalyzed by transglutaminase, the efficient extraction of elastin, preservation of the natural cross-linked structure, and enhancement of water solubility are achieved.

[0039] Comparative Example 1, due to the elimination of biomimetic rumen pretreatment, did not effectively loosen the bone matrix structure, making it difficult to release elastin, resulting in a significant decrease in extraction rate and water solubility; Comparative Example 2, due to the lack of specific elastase, could not efficiently release elastin by relying solely on neutral protease, resulting in uneven molecular weight distribution of the product and low desmokinin content, fully demonstrating the necessity of the patented core process and components.

[0040] A liquid-to-solid mass ratio closer to 15:1, a fermentation time of 36-48 hours, and a specific elastase addition of 2%-3% result in higher elastin extraction rate, higher water-soluble transmittance, and higher deskeletalin content, leading to increased fibroblast proliferation rate. The liquid-to-solid mass ratio directly affects the contact efficiency between microorganisms and the bone matrix, the fermentation time determines the degree of bone matrix deconstruction, and the specific elastase addition affects the elastin release efficiency and molecular weight control. The synergistic effect of these three factors ensures the excellent performance of the final product.

[0041] Compared to Comparative Example 1, which omits the biomimetic rumen pretreatment, the extraction rate of the product in Example 1 is increased by more than 27%, the water-soluble transmittance is increased by more than 24%, the desmosin content is increased by more than 112%, and the fibroblast proliferation rate is increased by about 185%. Compared to Comparative Example 2, which does not use specific elastase, the performance indicators are significantly improved. At the same time, the entire process is mild and environmentally friendly, without strong acid or alkali pollution, and the finished product has good biocompatibility. When used in cosmetics, it has excellent anti-wrinkle and repair effects.

[0042] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A preparation process for cashmere goat bone-derived elastin, characterized in that, The steps are as follows: Raw material pretreatment; Bionic rumen pretreatment; Stepwise directional enzymatic hydrolysis; Hydrophilic modification and metabolic anchoring; Molecular interception and purification; The finished product is dried.

2. The preparation process of cashmere goat bone-derived elastin according to claim 1, characterized in that, The raw material pretreatment involves selecting long bones from the limbs of cashmere goats aged 12-24 months as raw materials, removing attached muscles, connective tissue, and bone marrow from the bone surface, and then crushing the bones into bone particles. The bone particles are placed in a multi-frequency ultrasonic cleaner and a degreasing solution made of ethanol and petroleum ether mixed in a volume ratio of 1:2 is added to remove the fatty components in the bone matrix. The degreased bone particles are washed with deionized water until neutral and then vacuum dried.

3. The preparation process of cashmere goat bone-derived elastin according to claim 1, characterized in that, The biomimetic rumen pretreatment involves constructing an anaerobic bioreactor that simulates the rumen environment of a cashmere goat. Artificial rumen fluid is added to the reactor, bone particles are introduced into the reactor, and the liquid-solid mass ratio is maintained at 10:1 to 15:

1. A mixture of carbon dioxide and nitrogen is introduced into the reactor to maintain the anaerobic environment and reduction potential, and a composite rumen microbial community is inoculated for fermentation.

4. The preparation process of cashmere goat bone-derived elastin according to claim 1, characterized in that, The stepwise directional enzymatic hydrolysis process involves first heating the reaction system to inactivate microorganisms after fermentation, then cooling it down and adding the first-stage enzyme preparation for neutral enzymatic hydrolysis to remove residual collagen and other proteins; after adjusting the pH of the system, the second-stage enzyme preparation is added for specific elastase hydrolysis.

5. The preparation process of cashmere goat bone-derived elastin according to claim 1, characterized in that, In the hydrophilic modification and metabolic anchoring step, when the second stage of enzymatic hydrolysis in the stepwise directional enzymatic hydrolysis has been carried out for 5 hours, the modified strain metabolites containing polyglutamic acid and transglutaminase are added to the reaction system.

6. The preparation process of cashmere goat bone-derived elastin according to claim 1, characterized in that, The molecular interception and purification process involves centrifuging the enzymatic hydrolysate to obtain the supernatant, which is then sequentially passed through a microfiltration membrane with a pore size of 0.45 μm, an ultrafiltration membrane with a molecular weight cutoff of 50 kDa, and a nanofiltration membrane with a molecular weight cutoff of 3-10 kDa for multi-stage filtration and concentration. The resulting concentrate is then passed through an electrodialysis device to remove inorganic salt ions, reducing the conductivity to below 50 μS / cm.

7. The preparation process of cashmere goat bone-derived elastin according to claim 1, characterized in that, The finished product drying step involves spray drying or vacuum freeze drying the purified cashmere goat bone elastin concentrate to obtain cashmere goat bone elastin powder with a moisture content of less than 3%.

8. The preparation process of cashmere goat bone-derived elastin according to claim 2, characterized in that, The multi-frequency ultrasonic cleaner uses frequency conversion technology to generate cavitation bubbles of different sizes to penetrate into the micropores of bone trabeculae. The ultrasonic power is set to 0.6 W / cm². 2 The ethanol in the degreasing solution is anhydrous ethanol, and the amount of the degreasing solution added must completely submerge the bone particles. After degreasing treatment, the triglycerides and phospholipids in the bone matrix are removed to a mass fraction of less than 0.5%.

9. The application of cashmere goat bone-derived elastin prepared according to any one of claims 1 to 8 in cosmetics, characterized in that: The cashmere goat bone-derived elastin is used as the core active ingredient, and its addition amount is 0.1% to 5.0% of the total mass of the cosmetic.

10. The application according to claim 9, characterized in that: In the cosmetic formulation system, the cashmere goat bone-derived elastin, small molecule sodium hyaluronate, and ceramide constitute a composite modification system; wherein, the molecular weight of small molecule sodium hyaluronate is 3kDa to 5kDa, and its mass ratio with elastin is 1:5; the ceramide is embedded in self-assembled nanomicelles formed by elastin peptides through a high-pressure homogenization process to form a transdermal delivery system with a particle size distribution between 100nm and 200nm.