Methanogenic lyases structurally homologous to PeiR lyase and their applications
By discovering methanogenic bacterial lysins that are structurally homologous to PeiR, the problem of high methane production in ruminants was solved, achieving effective methane inhibition and demonstrating its application potential in the field of methane emission reduction.
CN120400106BActive Publication Date: 2026-05-26ZHEJIANG UNIV
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Patent Information
- Application Number
- CN202510586875.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-08
- Publication Date
- 2026-05-26
- Estimated Expiration
- 2045-05-08
AI Technical Summary
Technical Problem
In existing technologies, ruminants produce a high amount of methane and lack effective methane inhibitors. The PeiR lyase has an unstable structure and is difficult to apply widely.
Method used
By collecting rumen metagenomic data, bioinformatics software was used to discover methanogenic lysins that are structurally homologous to PeiR, primers were designed for prokaryotic expression, crude enzyme solutions of recombinant proteins were obtained, and their in vitro methane generation inhibition effect was evaluated.
Benefits of technology
We successfully obtained a variety of methanogenic lyases with structures similar to PeiR, which significantly reduced methane production in rumen fluid, demonstrating their application potential in the field of methane emission reduction.
✦ Generated by Eureka AI based on patent content.
Abstract
This invention discloses a methanogenic lyase homologous to PeiR lyase and its applications. The PeiR lyase protein, targeting the peptide bonds of the methanogenic cell wall, participates in the biological process of hydrolyzing the archaea cell wall, effectively killing methanogens and reducing methane production. Proteins homologous to this lyase possess potential methane-reducing potential. This invention utilizes a series of bioinformatics software to identify protein sequences encoded by methanogenic viruses from a large amount of rumen microbial virome sequencing data, and analyzes the structural homology between these proteins and PeiR. Ultimately, this invention successfully obtained a series of methanogenic lyases homologous to PeiR. To achieve the expression of these lyases, this invention designed multiple primers to synthesize the target sequences and successfully expressed them in a prokaryotic expression system. In vitro gas production experiments with the crude enzyme solution showed that these lyases are effective in reducing methane production.
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