20 results about "Methanogen" patented technology

Animal feed compositions for reducing methane emission and / or for inhibiting one or more methane producing organisms (i.e., methanogens). Also included are the use of said compositions to reduce methane emission from animals such as ruminant animals, to inhibit methanogenesis by one or more methanogens in said animals, and / or to increase milk yield and / or body weight gain of said animals is described, as well as related methods.

According to the rice field carbon emission reduction and yield increase synergistic system and method, EC11 bacteria inhibit methane bacteria by occupying ecological niche, and micromolecule water treated by fullerene not only optimizes the soil microenvironment (the volume weight is reduced by 12%), but also can promote the metabolic activity of the EC11 bacteria (the respiration rate is increased by 25%) to form 1 + 1gt; and 2, the synergistic effect is achieved. Meanwhile, a carbon monitoring and trading system is pre-embedded into a planting process, and a'planting and trading 'mode of paddy field carbon assets is realized for the first time.

The present application belongs to the technical field of sewage treatment, and discloses a method for simultaneously producing medium-chain fatty acids and hydrogen by enhancing sludge anaerobic fermentation. The sludge is concentrated and mixed with alkaline biochar, and then potassium ferrate is added for reaction to obtain pretreated sludge. After adjusting the pH, the sludge is mixed with an electron donor and inoculated sludge for anaerobic fermentation to obtain a fermentation liquor rich in medium-chain fatty acids and hydrogen. The alkaline biochar-potassium ferrate coupling system is used to break down the extracellular polymeric substance and cell wall of the sludge, drive the dissolution of particulate organic matter, and inhibit the activity of methanogens. Fe2O3 derived from potassium ferrate forms an iron-carbon composite with biochar in situ, and by enhancing the efficiency of electron transfer and enzyme activity, the acidification process of organic matter and the carbon chain extension reaction are promoted, forming a composite technology with three functions of "pretreatment-electron regulation-methane inhibition", realizing the simultaneous recovery of medium-chain fatty acids and hydrogen, and improving the sludge treatment efficiency.

This invention discloses a method for improving methanogenesis from lignocellulose based on a mixed-culture fermentation strategy, belonging to the field of anaerobic fermentation technology. The method includes the following steps: (1) inoculating activated *Trichoderma echinosporum* into a culture medium containing lignocellulose and loofah sponge as a biological carrier material for fermentation to obtain a fermentation broth; (2) activating activated sludge using a revitalizing solution; (3) inoculating the activated sludge from step (2) into the fermentation broth of *Trichoderma echinosporum* from step (1) for anaerobic fermentation to produce methanogens. This method achieves a methane yield of 262 mL / g VS, which is more than 30% higher than that of traditional anaerobic fermentation for methanogenesis, and is of great significance for improving the bioconversion of lignocellulose resources.

This invention provides an amorphous iron-manganese oxide cluster, its preparation method, and its application in enhancing methanogenesis through functional enzymes. It belongs to the interdisciplinary technical fields of environmental functional materials, waste biological treatment, and resource utilization. The invention first mixes manganese salt and hydrochloric acid solution to obtain a hydrochloric acid solution of manganese salt; dissolves iron salt in water to obtain an iron salt solution; mixes the hydrochloric acid solution of manganese salt and the iron salt solution, heats the mixture, and then adds isopropanolamine solution to react, obtaining amorphous iron-manganese oxide clusters. The dispersion of the above amorphous iron-manganese oxide clusters is added to mixed bacterial granular sludge for sequential activation and anaerobic digestion. This can serve as an enhancer and protectant for anaerobic microbial functional enzymes, eliminating the influence of intermediate acids on the methanogenesis process through chelation or adsorption. It can also increase the adenine triphosphate yield of methanogenic archaea, accelerate archaea growth, and balance the growth differences between archaea and acidogenic bacteria.

The invention discloses a new application of an ETD1 gene and a method for reducing the abundance of rhizosphere methane bacteria and reducing methane emission. ETD1 is a superallele of OsCNGC13, is a recessive mutant, and remarkably reduces the abundance of rhizosphere methane bacteria by mediating root calcium ion influx, so that the methane emission of the rice field is reduced by 60-80%. The invention also provides a nucleic acid molecule containing the ETD1 gene, a protein, a recombinant vector, an expression cassette and application thereof in cultivation of low-methane-emission rice varieties. In addition, the invention provides an OsCNGC13 gene knockout method, an ETD1 recombinant vector construction method, an ETD1 mutant screening method and an ETD1 specific molecular marker identification method. The invention provides a new gene resource and a breeding technology for synergistically improving the disease resistance and the low-carbon emission character of the rice, and has important agricultural production and environmental application values.

The application discloses a kind of carbon source preparation devices of kitchen wastewater system, apply in carbon source preparation field, including directional fermentation carbon source tank, sediment tank and in situ desulfurization anaerobic tank, the surface of directional fermentation carbon source tank is connected with organic slurry water inlet pipe, biogas slurry backflow pipe and liquid alkali pipe, the surface of directional fermentation carbon source tank is connected with first air blower;The application is in micro-oxygen environment by aeration control hydrolysis acidification, inhibits the activity of methanogen, domesticates hydrolysis bacteria, simultaneously cultivate aerobic bacteria to form bacteria agglutination, realize that slurry has good sedimentation performance, SV30 reaches 40%~60%.Carbon source slurry with good sedimentation performance is separated by integrated slag scraping sedimentation machine, the separated effluent SS is controlled between 1000~3000mg / L, and the SS of effluent is better than the effect of two centrifuges.

This invention discloses ETD1 Novel uses of genes and methods to reduce rhizosphere methanogen abundance and methane emissions. ETD1 yes OsCNGC13 The superalle, a recessive mutant, significantly reduces rhizosphere methanogen abundance by mediating root calcium ion influx, thereby reducing methane emissions from paddy fields by 60-80%. This invention also provides a [missing information - likely a specific gene or formula]. ETD1 This invention relates to nucleic acid molecules, proteins, recombinant vectors, expression cassettes of genes, and their applications in breeding low-methane-emission rice varieties. Furthermore, this invention provides... OsCNGC13 Gene knockout methods ETD1 Recombinant vector construction methods ETD1 Mutant screening methods and ETD1 ETD1 ETD1 ETD1 ETD1 ETD1 ETD1 ETD1 ETD1 ETD1 A specific molecular marker identification method. This invention provides new gene resources and breeding techniques for the synergistic improvement of disease resistance and low carbon emission traits in rice, and has significant value for agricultural production and environmental applications.

The utility model discloses a kitchen waste digestive juice flocculation system which comprises a storage tank and a conditioning pool unit, the storage tank comprises a tank body and a temperature regulating device, and the temperature regulating device is arranged on the tank body and used for regulating the temperature of digestive juice in the tank body; the conditioning tank unit comprises a conditioning tank, a first liquid inlet pipe, an aeration pipe and a first flocculant adding device, the first liquid inlet pipe is communicated with the conditioning tank and the tank body, the air outlet end of the aeration pipe is arranged in the conditioning tank, and the discharging end of the first flocculant adding device is communicated with the conditioning tank. Compared with the prior art, the storage tank has the advantages that the temperature regulation and control device is arranged on the tank body of the storage tank, digestive juice is rapidly cooled after entering the tank body, temperature mutation is formed, the biological activity of methane bacteria is inhibited, the generation amount of methane gas is reduced, aeration is performed in the conditioning tank, anaerobic methane bacteria are killed, the generation amount of the methane gas is further reduced, and the methane gas quality is improved. Therefore, the hindering effect of the methane gas on the flocculating agent is weakened, and the usage amount of the flocculating agent is reduced.

The invention discloses a compound functional bacterium preparation as well as an enrichment method and application thereof in an AnMBR (Anaerobic Membrane Biological Reactor), and belongs to the technical field of sewage anaerobic membrane biological treatment. According to the invention, anaerobic sludge is used as a matrix, gamma-caprolactone (GCL) and glucose are used as a composite carbon source for pulse supply under the condition of electrical stimulation assistance, and a two-stage enrichment and gradient screening process is combined, so that the composite functional bacteria with quorum sensing quenching (QQ) and methane production capabilities are obtained; and then fixing the composite microspheres in sodium alginate-chitosan-Fe < 3 + > composite microspheres for AnMBR addition operation. The electrical stimulation enhances the degradation of AHLs and the proliferation of methane bacteria in the enrichment stage, accelerates the formation of flora, and improves the biological stability of the system and the yield of methane. Compared with an existing functional bacterium enrichment method, electrical stimulation is introduced in the enrichment stage to serve as a metabolism regulation means, the flora function expression level is remarkably improved, and the method is a core technical path for achieving membrane pollution relieving and methane productivity synergistic improvement.

A waste incineration facility (1) is provided with: an exhaust gas flow path (4) through which exhaust gas discharged from an incinerator (3) flows; a wet scrubbing tower (43) provided in the exhaust gas flow path (4); a recirculating exhaust gas line (6) which is connected to an extraction position (P1) on the downstream side of the exhaust gas flow path (4) than the wet-type smoke washing tower (43), extracts a part of the exhaust gas flowing through the exhaust gas flow path (4) as recirculating exhaust gas, and supplies the recirculating exhaust gas into the incinerator (3); an oxygen mixing unit (66) that mixes high-concentration oxygen with the recirculation exhaust gas flowing through the recirculation exhaust gas line (6); and a methanation device (51) that generates a methane-containing gas by reacting the exhaust gas, which passes through the extraction position (P1) and flows through the exhaust gas flow path (4), with hydrogen gas. The recirculated exhaust gas supplied into the incinerator (3) is the main gas of the combustion gas used for incinerating waste in the incinerator (3).

The present invention relates to an ex-situ biomethanation process and an apparatus for carrying out the process. The present invention relates to an ex-situ methane production process comprising the step of contacting at least one methanogen, a culture medium and optionally a first culture medium in a gas / liquid bioreactor, said gas / liquid bioreactor optionally comprising a second culture medium, and producing methane by reacting the influent gas with at least one methanogenic microorganism, characterized in that the culture medium is optionally immobilized in a gas / liquid bioreactor; and at least one methanogenic microorganism immobilized on the culture medium; the invention also relates to a device for carrying out the method.

The invention relates to the technical field of ruminant methane emission prediction and regulation and control, in particular to a ruminant rumen methane emission prediction and regulation and control system based on dynamic Monod model and multi-algorithm collaborative optimization. S1, data acquisition and preprocessing; s2, FOM calculation is carried out; s3, the generation amount of H2 and the generation amount of CO2 are calculated; s4, calculating a methanogen kinetic model; s5, multi-algorithm collaborative optimization including simulated annealing and a genetic algorithm; and S6, outputting a result and generating a strategy. According to the method, high-sensitivity parameters can be preferentially regulated and controlled, so that the prediction precision is remarkably improved, the parameter optimization efficiency and the global optimal solution search capability are remarkably enhanced, and the wide applicability and the scene adaptability are better.

Disclosed herein are polynucleotides encoding one or more rumen-associated antigens, and polypeptides encoded by the polynucleotides. Also provided herein are compositions comprising the same, as well as methods of making and using the compositions. The present disclosure identifies particular challenges regarding rising global surface temperatures due to increasing levels of the greenhouse gas, methane (CH4). For example, the present disclosure identifies that increasing agricultural methane emissions represent a major source of greenhouse gas emissions. The present disclosure also identifies that reducing methane emissions from animals, such as ruminants, will be important for reducing greenhouse gas emissions.

The application discloses a composite functional bacteria preparation, an enrichment method thereof and application of the composite functional bacteria preparation in an AnMBR, and belongs to the technical field of sewage anaerobic membrane biological treatment. The application adopts anaerobic sludge as a substrate, provides gamma-caprolactone (GCL) and glucose as a composite carbon source pulse supply under the condition of electric stimulation assistance, combines two-stage enrichment and gradient screening processes, and obtains composite functional bacteria which has both quorum quenching (QQ) and methanogenesis capacity. 3+ The composite microspheres are used for AnMBR dosing operation. The electric stimulation strengthens degradation of AHLs and proliferation of methanogens in the enrichment stage, accelerates formation of a bacterial community, and improves biological stability and methane production of the system. Compared with existing functional bacteria enrichment methods, the application introduces electric stimulation as a metabolic regulation means in the enrichment stage, significantly improves the functional expression level of the bacterial community, and is a core technical path for realizing membrane pollution mitigation and collaborative improvement of methane production capacity.

Disclosed is a composition for inhibiting methane production in ruminants, comprising a compound having chemical structural formula I, or a salt, a solvate, a hydrate, or an isomer thereof. The compound binds to a protein structure of methyl-coenzyme M reductase (MCR), which is a methanogen enzyme in the rumen of a ruminant, and inhibits enzymatic activity, thereby reducing methane production. In addition, a feed additive and a feed for ruminants comprising same are disclosed.

A method for achieving a remediation of a chlorinated organic pollutant (COP)-contaminated soil and a reduction of a methane emission synchronously through a microbial regulation is provided, including: in an anaerobic environment, adding at least one of electron donors, electron shuttles, and electron flow interference agents to the COP-contaminated soil to accurately and directionally regulate an electron transfer route, facilitating a COP reductive dechlorination mediated by indigenous microorganisms and meanwhile inhibiting a growth and a metabolism of methanogens synchronously. Electron donors are added as a carbon source to promote microbial growth and metabolism under anaerobic conditions, electron shuttles such as biochar are added to accelerate the transfer of electrons from the donor to the acceptor, and the electron flow interference agents such as a methanogenic inhibitor or a FeCl3 solvent are added to regulate more electrons released from microbial respiratory metabolism to flow towards dechlorination process rather than methanogenesis process.

The invention discloses a kangaroo-derived microorganism targeted bovine stomach methane emission reduction device and method. The device comprises a sterile sampling tool, intelligent algorithm regulation and control equipment, a constant-temperature incubator, a microscope, a centrifugal machine, a bioreactor, a high-speed mixer and a gas chromatograph. The method comprises the following steps: collecting a sample from juvenile kangaroo excrement, and separating and purifying microorganisms by using a sterile sampling tool; culturing the separated special microorganisms in a constant-temperature incubator; introducing the cultured microorganisms into a bioreactor for simulating the cattle stomach environment, and observing the interaction between the cultured microorganisms and original methanogens; the method comprises the following steps: uniformly mixing microorganisms with a carrier by using a high-speed mixer to prepare a microbial preparation suitable for feeding, and filling the microbial preparation into a sealed container; a gas chromatograph is used for monitoring the methane emission amount of the cattle, and intelligent algorithm regulation and control equipment is used for timely adjusting a preparation method and a feeding scheme of a microbial preparation; and an efficient, specific and sustainable animal husbandry greenhouse gas emission reduction strategy is realized.

The invention discloses an expansin mutant with a function of inhibiting methane bacteria as well as a coding gene and application of the expansin mutant, and belongs to the technical field of biological genetic engineering. The invention provides an expansin mutant which comprises a mutant PS1-M1 and a mutant PS1-M2, the mutant PS1-M2 is obtained by mutating 173rd-site serine of PFSWO11 expansin-like protein into alanine and mutating 176th-site glutamine into phenylalanine, hydrophobic amino acid sites in the protein are replaced, so that the hydrophobicity of the protein is reduced or spatial rearrangement occurs, and the expansin-like protein is obtained. On the basis of maintaining the structural stability, the mutant PSI-M1 and the mutant PSI-M2 have the ability of inhibiting the growth or metabolic activity of rumen methanogenic archaea, so that the rumen methane generation is reduced, the feed energy utilization efficiency is improved, a new biological technical approach is provided for ruminant methane emission reduction, and the mutant PSI-M1 and the mutant PSI-M2 have popularization significance.

The invention belongs to the technical field of biological energy and organic solid waste resourceful treatment, and provides a strengthening method for anaerobic methanogenesis. According to the method, magnetotactic bacteria Solidesulfovibrio magneticus RS-1 are introduced into an anaerobic methanogenesis system containing methanogenesis archaea, and a magnetic conductive material is added, so that the magnetotactic bacteria are directionally enriched on the surface of a magnetic material under the magnetic action of the magnetic conductive material and a local magnetic environment formed by the magnetic conductive material; and forming a microbial aggregate with a magnetic material as a core with methanogenic archaea, so that an efficient electron transfer link is constructed in a micro-domain scale, and the methane generation efficiency is improved. The embodiment shows that compared with a control system without magnetotactic bacteria and magnetic materials, the method has the advantages that the methane accumulation yield can be remarkably increased by 30% or above, enrichment of methanogens in magnetic aggregates is promoted, the mass transfer resistance of intermediate metabolites is reduced, and the stability of the system is enhanced.