10 results about "Methanogen" patented technology

According to the rice field carbon emission reduction and yield increase synergistic system and method, EC11 bacteria inhibit methane bacteria by occupying ecological niche, and micromolecule water treated by fullerene not only optimizes the soil microenvironment (the volume weight is reduced by 12%), but also can promote the metabolic activity of the EC11 bacteria (the respiration rate is increased by 25%) to form 1 + 1gt; and 2, the synergistic effect is achieved. Meanwhile, a carbon monitoring and trading system is pre-embedded into a planting process, and a'planting and trading 'mode of paddy field carbon assets is realized for the first time.

The present application belongs to the technical field of sewage treatment, and discloses a method for simultaneously producing medium-chain fatty acids and hydrogen by enhancing sludge anaerobic fermentation. The sludge is concentrated and mixed with alkaline biochar, and then potassium ferrate is added for reaction to obtain pretreated sludge. After adjusting the pH, the sludge is mixed with an electron donor and inoculated sludge for anaerobic fermentation to obtain a fermentation liquor rich in medium-chain fatty acids and hydrogen. The alkaline biochar-potassium ferrate coupling system is used to break down the extracellular polymeric substance and cell wall of the sludge, drive the dissolution of particulate organic matter, and inhibit the activity of methanogens. Fe2O3 derived from potassium ferrate forms an iron-carbon composite with biochar in situ, and by enhancing the efficiency of electron transfer and enzyme activity, the acidification process of organic matter and the carbon chain extension reaction are promoted, forming a composite technology with three functions of "pretreatment-electron regulation-methane inhibition", realizing the simultaneous recovery of medium-chain fatty acids and hydrogen, and improving the sludge treatment efficiency.

This invention discloses a method for improving methanogenesis from lignocellulose based on a mixed-culture fermentation strategy, belonging to the field of anaerobic fermentation technology. The method includes the following steps: (1) inoculating activated *Trichoderma echinosporum* into a culture medium containing lignocellulose and loofah sponge as a biological carrier material for fermentation to obtain a fermentation broth; (2) activating activated sludge using a revitalizing solution; (3) inoculating the activated sludge from step (2) into the fermentation broth of *Trichoderma echinosporum* from step (1) for anaerobic fermentation to produce methanogens. This method achieves a methane yield of 262 mL / g VS, which is more than 30% higher than that of traditional anaerobic fermentation for methanogenesis, and is of great significance for improving the bioconversion of lignocellulose resources.

The invention discloses a new application of an ETD1 gene and a method for reducing the abundance of rhizosphere methane bacteria and reducing methane emission. ETD1 is a superallele of OsCNGC13, is a recessive mutant, and remarkably reduces the abundance of rhizosphere methane bacteria by mediating root calcium ion influx, so that the methane emission of the rice field is reduced by 60-80%. The invention also provides a nucleic acid molecule containing the ETD1 gene, a protein, a recombinant vector, an expression cassette and application thereof in cultivation of low-methane-emission rice varieties. In addition, the invention provides an OsCNGC13 gene knockout method, an ETD1 recombinant vector construction method, an ETD1 mutant screening method and an ETD1 specific molecular marker identification method. The invention provides a new gene resource and a breeding technology for synergistically improving the disease resistance and the low-carbon emission character of the rice, and has important agricultural production and environmental application values.

This invention discloses ETD1 Novel uses of genes and methods to reduce rhizosphere methanogen abundance and methane emissions. ETD1 yes OsCNGC13 The superalle, a recessive mutant, significantly reduces rhizosphere methanogen abundance by mediating root calcium ion influx, thereby reducing methane emissions from paddy fields by 60-80%. This invention also provides a [missing information - likely a specific gene or formula]. ETD1 This invention relates to nucleic acid molecules, proteins, recombinant vectors, expression cassettes of genes, and their applications in breeding low-methane-emission rice varieties. Furthermore, this invention provides... OsCNGC13 Gene knockout methods ETD1 Recombinant vector construction methods ETD1 Mutant screening methods and ETD1 ETD1 ETD1 ETD1 ETD1 ETD1 ETD1 ETD1 ETD1 ETD1 A specific molecular marker identification method. This invention provides new gene resources and breeding techniques for the synergistic improvement of disease resistance and low carbon emission traits in rice, and has significant value for agricultural production and environmental applications.

The invention relates to the technical field of ruminant methane emission prediction and regulation and control, in particular to a ruminant rumen methane emission prediction and regulation and control system based on dynamic Monod model and multi-algorithm collaborative optimization. S1, data acquisition and preprocessing; s2, FOM calculation is carried out; s3, the generation amount of H2 and the generation amount of CO2 are calculated; s4, calculating a methanogen kinetic model; s5, multi-algorithm collaborative optimization including simulated annealing and a genetic algorithm; and S6, outputting a result and generating a strategy. According to the method, high-sensitivity parameters can be preferentially regulated and controlled, so that the prediction precision is remarkably improved, the parameter optimization efficiency and the global optimal solution search capability are remarkably enhanced, and the wide applicability and the scene adaptability are better.

The application discloses a composite functional bacteria preparation, an enrichment method thereof and application of the composite functional bacteria preparation in an AnMBR, and belongs to the technical field of sewage anaerobic membrane biological treatment. The application adopts anaerobic sludge as a substrate, provides gamma-caprolactone (GCL) and glucose as a composite carbon source pulse supply under the condition of electric stimulation assistance, combines two-stage enrichment and gradient screening processes, and obtains composite functional bacteria which has both quorum quenching (QQ) and methanogenesis capacity. 3+ The composite microspheres are used for AnMBR dosing operation. The electric stimulation strengthens degradation of AHLs and proliferation of methanogens in the enrichment stage, accelerates formation of a bacterial community, and improves biological stability and methane production of the system. Compared with existing functional bacteria enrichment methods, the application introduces electric stimulation as a metabolic regulation means in the enrichment stage, significantly improves the functional expression level of the bacterial community, and is a core technical path for realizing membrane pollution mitigation and collaborative improvement of methane production capacity.

Disclosed is a composition for inhibiting methane production in ruminants, comprising a compound having chemical structural formula I, or a salt, a solvate, a hydrate, or an isomer thereof. The compound binds to a protein structure of methyl-coenzyme M reductase (MCR), which is a methanogen enzyme in the rumen of a ruminant, and inhibits enzymatic activity, thereby reducing methane production. In addition, a feed additive and a feed for ruminants comprising same are disclosed.

The invention discloses an expansin mutant with a function of inhibiting methane bacteria as well as a coding gene and application of the expansin mutant, and belongs to the technical field of biological genetic engineering. The invention provides an expansin mutant which comprises a mutant PS1-M1 and a mutant PS1-M2, the mutant PS1-M2 is obtained by mutating 173rd-site serine of PFSWO11 expansin-like protein into alanine and mutating 176th-site glutamine into phenylalanine, hydrophobic amino acid sites in the protein are replaced, so that the hydrophobicity of the protein is reduced or spatial rearrangement occurs, and the expansin-like protein is obtained. On the basis of maintaining the structural stability, the mutant PSI-M1 and the mutant PSI-M2 have the ability of inhibiting the growth or metabolic activity of rumen methanogenic archaea, so that the rumen methane generation is reduced, the feed energy utilization efficiency is improved, a new biological technical approach is provided for ruminant methane emission reduction, and the mutant PSI-M1 and the mutant PSI-M2 have popularization significance.

The invention belongs to the technical field of biological energy and organic solid waste resourceful treatment, and provides a strengthening method for anaerobic methanogenesis. According to the method, magnetotactic bacteria Solidesulfovibrio magneticus RS-1 are introduced into an anaerobic methanogenesis system containing methanogenesis archaea, and a magnetic conductive material is added, so that the magnetotactic bacteria are directionally enriched on the surface of a magnetic material under the magnetic action of the magnetic conductive material and a local magnetic environment formed by the magnetic conductive material; and forming a microbial aggregate with a magnetic material as a core with methanogenic archaea, so that an efficient electron transfer link is constructed in a micro-domain scale, and the methane generation efficiency is improved. The embodiment shows that compared with a control system without magnetotactic bacteria and magnetic materials, the method has the advantages that the methane accumulation yield can be remarkably increased by 30% or above, enrichment of methanogens in magnetic aggregates is promoted, the mass transfer resistance of intermediate metabolites is reduced, and the stability of the system is enhanced.