70 results about "Bioenergy" patented technology

A bioenergy generator to increase the life force of a biological system is provided. The bioenergy generator includes fine pieces of natural stone, fine pieces of natural sand, fine pieces of metal, water and a supporting material operable to hold the natural sand, the natural stone, the metal, and the water together. Uses of the bioenergy generator are also provided, in particular, to enhance stem cell production.

A bioenergy generator to increase the life force of a biological system is provided. The bioenergy generator includes fine pieces of natural stone, fine pieces of natural sand, fine pieces of metal, water and a supporting material operable to hold the natural sand, the natural stone, the metal, and the water together. Uses of the bioenergy generator are also provided, in particular, to enhance stem cell production.

Certain exemplary embodiments can provide systems and / or methods for producing dried biomass materials, such as from bales of biomass. Biomass materials can be converted into, e.g., pellets, briquettes, cubes, and / or other products with dried and / or densified states. These dried, densified products can be used for, e.g., bioenergy and / or animal feed applications.

The invention discloses an endoglucanase truncated mutant and an application of the endoglucanase truncated mutant. According to the invention, based on endoglucanase BsEGL from bacillus subtilis DLG, a truncated mutant is constructed by deleting a carbohydrate binding domain (CBM) of the endoglucanase BsEGL, and the truncated mutant is named as BsEGL [delta] CBM. Compared with BsEGL, the truncated mutant BsEGL delta CBM has the advantages that the thermal stability, the pH stability, the organic solvent tolerance and the like are remarkably improved, and the activity of degrading phosphoric acid expanded cellulose and corncob xylan is obtained. In addition, the enzymatic activity of the truncated mutant BsEGL delta CBM is remarkably improved by 6.41%, and the catalytic efficiency is remarkably improved. Due to the characteristics, the mutant can be suitable for industrial processes such as feed processing, papermaking, biological energy sources, spinning and food brewing, and is particularly suitable for enzyme catalytic reaction under extreme environments such as high temperature and low pH. The invention provides an enzyme preparation tool with excellent performance for efficient degradation of cellulose biomass, and has important application value.

A bioenergy generator to increase the life force of a biological system is provided. The bioenergy generator includes fine pieces of natural stone, fine pieces of natural sand, fine pieces of metal, water and a supporting material operable to hold the natural sand, the natural stone, the metal, and the water together. Uses of the bioenergy generator are also provided, in particular, to treat long-COVID and influenza.

The invention relates to a lignocellulose biomass glucose production prediction method based on machine learning, and aims to improve the yield of glucose and optimize a biomass conversion process. According to the method, machine learning models such as a multi-layer perceptron, support vector regression, a random forest, a gradient boosting decision tree and a convolutional neural network are adopted to predict the LCB glucose yield processed by different preprocessing modes. Experimental results show that the convolutional neural network model is optimal in performance, the decision coefficient Rreaches up to 0.95, and the method has excellent generalization ability and prediction precision. The prediction precision is remarkably improved by feature extraction and principal component analysis in the model establishment process, and the Rof the convolutional neural network model is improved from 0.76 to 0.95. The efficient and accurate prediction method is provided for optimization of the biomass saccharification process, the method can be widely applied to the field of biological energy, and technical support is provided for improving the resource utilization efficiency of lignocellulose biomass.

The invention discloses a biological energy feed fermentation yeast based on enzyme-mineral synergistic activation and a preparation method thereof. According to the scheme, dual functions of mineral activation and microbial conversion are focused, clinoptilolite, sodium bentonite and kaolin are taken as mineral carriers, lignite humic acid is taken as a nutrient synergist, brown sugar, rice bran and bran organic matrixes are matched, and mineral lattice structures are broken through mechanical crushing and biological enzymolysis pretreatment, so that the mineral active substances are obtained. And through a double-stage fermentation process of'aerobic fermentation to activate flora and anaerobic aging to enrich metabolites' of microorganisms, mineral ions, humic acid active ingredients and probiotic metabolites are released to the maximum extent, and finally, the high-quality biological energy feed fermentation yeast with nutrition supply, digestion promotion and immune regulation functions is formed. According to the bioenergy feed prepared by conversion of the fermentation yeast, the feed crude protein improvement rate is greater than or equal to 20%, the crude fiber degradation rate is greater than or equal to 40%, the feed conversion rate is improved by 10-15%, the intestinal disease incidence rate is reduced by 60-70%, and the bioenergy feed is suitable for various livestock and poultry and aquaculture scenes.

The invention relates to the technical field of microbial culture, in particular to a heterotrophic culture method of benthic diatom, which comprises the following steps: S1, adding benthic diatom into an improved SE culture medium, and culturing for 5-7 days until OD540 is 0.8-1.2 to obtain a benthic diatom seed solution; according to the method disclosed by the invention, a nutrition system is constructed by taking waste glycerol as a carbon source and sodium alginate coated urea as a slow-release nitrogen source, and the benthic diatom, nitrogen-fixing bacteria and phosphate solubilizing bacteria are co-cultured on a porous ceramic carrier to be cultured under a dark condition, so that compared with the prior art, the method disclosed by the invention thoroughly gets rid of the dependence on illumination, and the yield is increased. The rapid, high-yield and stable heterotrophic culture of the benthic diatom is realized, meanwhile, the raw material and energy consumption cost is greatly reduced, and the resource recycling rate is improved; the obtained diatom biomass can be used for aquatic bait, biological energy, environmental restoration and development of high-added-value products, and has a wide industrial application prospect.

The present disclosure relates to methods, devices and systems for exposing microorganisms or compositions comprising functional proteins to an oscillating electric field substantially free of a magnetic component in order to improve stress responses, survival and metabolite or protein production. In embodiments, a controller drives one or more insulated emitters with a true AC signal or pulsed DC signal, in stepped, swept or shuffled patterns of an electric field oscillating at one or more frequencies in the range 210 Hz to 99 kHz. Exposure to the field provides surmounting energy that enhances protein breathing, catalysis, and fundamental functions of multiprotein complexes including transcription factors and ribosomes, thereby improving stress resistance of microorganisms, their viability during fermentation, drying, storage, transport, and / or increasing production of functional or therapeutic proteins, metabolites or microbial lysates. The technology is applicable to manufacture of probiotics, therapeutics, metabolites, food, alcohol, feed and bioenergy, cosmetics, nutraceuticals and biopharmaceuticals..

The invention relates to the technical field of phytoremediation of soil polluted by organophosphate flame retardants / plasticizers, in particular to a method for remedying soil polluted by chlorinated organophosphate by using compositae plant jerusalem artichoke. The compositae plant jerusalem artichoke is planted in the soil polluted by the chlorinated organophosphate, and the plant degrades, removes or remarkably reduces the content of phosphate tri (2-chloroethyl) ester, phosphate tri (1-chloro-2-propyl) ester and phosphate tri (1, 1-dichloro-2-propyl) ester in the soil through the synergistic effect of absorption and root system secreted substances in the growth process. And the content of chlorinated organophosphorus esters such as 2, 3-dichloro-2-propyl) ester and the like is increased. According to the method, the energy plant jerusalem artichoke capable of being used for producing bioethanol or methane is adopted for repairing the soil polluted by the chlorinated organophosphate, the method has the advantages of being small in investment, not high in technical requirement, capable of being used for in-situ repairing and the like, and the situation that the chlorinated organophosphate in the soil enters a food chain through plants and then harms human health can be avoided; meanwhile, raw materials can be provided for production of biological energy.

The present application discloses a biofuel and a preparation method thereof, and relates to the field of bioenergy technology. A method for preparing biofuel comprises the following steps: drying and crushing the biomass raw material, using an acetone system as a reaction medium, adding an ionic liquid catalyst, heating to carry out a liquefaction reaction, and obtaining a liquefied product; centrifuging and washing the liquefied product, and then performing rotary evaporation to obtain a biofuel precursor; adding a hydrogen donor, a zero-valent metal, a hydrogenation catalyst, and water to the biofuel precursor, heating and stirring in a closed environment, and performing a hydrogenation deoxygenation reaction to obtain a reaction product; filtering and rotary evaporating the reaction product to obtain biofuel. The present application realizes the conversion from biomass to biofuel, and the oxygen content of the prepared biofuel is greatly reduced, and the calorific value is significantly improved, which provides a possibility for replacing some non-renewable energy sources.

The present invention belongs to the technical field of molecular biology, and specifically relates to a xylanase XynZT-2 mutant and its application. The xylanase XynZT-2 mutant is obtained by site-directed mutagenesis of the xylanase XynZT-2 with the amino acid sequence shown in SEQ ID No. 2. The specific mutation sites are as follows: T28I, A33V, M97L, A102C, S148T, A238G, S239P, M241L, T287S or I291V. The optimal temperature and optimal pH of 10 xylanase XynZT-2 mutants have changed, and the obtained xylanase XynZT-2 mutants show application value in the fields of pulp brewing, bioenergy, food manufacturing, etc.

The present disclosure teaches an algal growth system. The algal growth system includes a flexible sheet material, a liquid reservoir, a mechanism to rotate the flexible sheet material, a resource reclamation device, and an enclosure. The flexible sheet material facilitates growth of a biofilm. The liquid reservoir is configured to retain a contacting liquid in contact with the flexible sheet material and the mechanism rotates the flexible sheet material through the contacting liquid.The resource reclamation device associated with the enclosure is configured to collect and provide a resource for reuse.

The utility model relates to the technical field of biological energy conversion, in particular to fertilizer production equipment with a biological energy conversion function, which comprises a conversion worktable, and a conversion component is arranged at the top of the conversion worktable. According to the fertilizer production equipment with the biological energy conversion function, by installing the conversion assembly, when fishes are treated, the fishes are poured into a smashing barrel firstly, then a driving motor is used for driving a smashing blade on a driving shaft to rotate, and therefore the fishes can be smashed and then discharged into a conversion box; meanwhile, the electric heating pipe is controlled to heat, so that biomass can be converted into biochar by utilizing high temperature, anaerobic digestion treatment is performed on the biochar, organic fertilizer can be obtained, then the fertilizer is conveyed into the reaction box through the discharging pipe to be subjected to organic conversion treatment, and usable organic fertilizer is obtained; the effective utilization and recycling of resources are realized, and the waste of the resources and the pollution to the environment are avoided.

Various examples relate to a transgenic crop plant where a sequence of a cis-regulatory element in the promoter of certain genes is genomically changed in order to alter the flowering time or flowering rate of the plant. In one example, the cis-regulatory element in the promoter of such certain genes may be modified from TGAATG(A / T) (A / T / C) to TGAGGG(A / T) (A / T / C). The certain genes may be genes that encode SbEhd1, OsFTL10 / FT8, SbFT10, ZmELF3.2, or combinations thereof. And, the crop plant can be for example, sorghum, maize or rice. In another example, the present transgenic crop plant is a progeny plant. In yet another example, flowering time of the transgenic crop plant is regulated by a method that includes changing the sequence of the cis-regulatory element in the promoter of certain genes.

The application discloses a preparation method of a copper-based catalyst, the copper-based catalyst and application thereof, and belongs to the technical field of bioenergy and chemical industry. The method comprises the following steps: obtaining a precursor I; the precursor I comprises a carrier and a supported active component, the active component contains copper elements, and the carrier is a molecular sieve with a silicon-aluminum ratio greater than 200; the precursor I is subjected to silicon component coating treatment to obtain a precursor II; the precursor II is calcined and reduced to obtain the copper-based catalyst; the silicon component coating treatment comprises the following steps: obtaining a gel containing a silicon source I, a template agent I, a solvent I and the precursor I; and the gel is heated in water vapor to obtain the precursor II. The method has the advantages of simple operation, low catalyst cost, economy and practicability, high acetaldehyde production efficiency and low energy consumption.

The invention discloses a growth conditioner for planting tricholoma matsutake and a preparation method thereof, and belongs to the technical field of tricholoma matsutake planting.The growth conditioner for planting tricholoma matsutake is prepared from, by weight, 5-8 parts of penicillium citrinum metabolite, 2 parts of arbuscular mycorrhizal fungi with the spore content being 20-30 / g, 5 parts of bacillus subtilis 108-1010 CFU / g and 10-12 parts of matrix. The pH value of the saline-alkali soil can be reduced, meanwhile, the organic matter content of the soil is increased, and the method is suitable for planting tricholoma matsutake; the beta-glucosidase activity of the tricholoma matsutake is promoted, the biomass of cell walls of pine trees serving as host trees is easily utilized, biological energy is obtained, and the growth of the tricholoma matsutake is promoted.

The utility model relates to the technical field of biological energy, and discloses efficient fatty acid methyl ester mixing equipment for biodiesel, which comprises a barrel, a first bolt penetrates through the upper surface of the barrel in a threaded manner, and a barrel cover, a separation plug, a precipitation tube and a stirring shaft penetrate through the barrel in a threaded manner through the first bolt. The device is reasonable in structure, liquid flows into the precipitation funnel, flows into the precipitation box assembly after being filtered for the first time, extracts precipitates through the second water pump, unscrews the separation plug to enable glycerol to flow into the glycerol barrel, and finished liquid in the separation barrel flows into the finished liquid barrel through the third material passing pipe. The high-pressure spray head is sprayed into the barrel, the cleaning liquid flows into the settling box assembly, reactant residues in the barrel due to long-time reaction are avoided, and the quality of the finished product is not influenced due to the fact that the high-pressure spray head is sprayed into the barrel and the cleaning liquid flows into the settling box assembly. And the working efficiency is reduced, the quality is improved, and the time is shortened.

The present application relates to a kind of lytic polysaccharide monooxygenase mutant and cellulose synergistic hydrolysis method.The present application belongs to the field of biotechnology, and traditional cellulose hydrolysis technology has the problems of low efficiency and high cost, the present application obtains mutant with higher catalytic activity and stability by site-directed mutagenesis to the 2nd and 43rd positions of the amino acid sequence shown in SEQ ID No:1, significantly improves cellulose hydrolysis efficiency, and has wide application prospect in the fields of bioenergy, papermaking, textile and the like.

The invention discloses an artificial multicellular system and application thereof, and belongs to the technical field of microorganisms. According to the method, the optimal inoculation ratio of the returning soil to the fresh cow dung is determined to be 1: 1, secondary screening of a bacterial system is carried out under the condition, an artificial multicellular system is obtained, the final accumulated total methane yield reaches 560.39 mL / (gVS) and the methane yield is 36.20 mL / (gVS.d) when the artificial multicellular system is used for fermentation, and the methane yield is improved by 34.17% compared with that of a primary generation. Compared with a single-environment inoculum, the yield of methane in a TF group is increased by 68.76% compared with T-P1 (primary-generation only returning-to-field soil) and is increased by 58.01% compared with F-P1 (primary-generation only fresh cow dung). According to the method, the lignocellulose hydrolysis efficiency and the methane conversion capacity are effectively improved, and a new way is provided for lignocellulose waste treatment and biological energy source production.

The invention relates to a bioenergy mixed-twisted filament and application thereof. The bioenergy mixed-twisted filament comprises bioenergy yarn, bioenergy fabric, products and the like made of the bioenergy mixed-twisted filament. The bioenergy mix-twisted filament comprises a first filament and a second filament which are mix-twisted, where the first filament comprises a first polymer matrix and nanoparticles dispersed in the first polymer matrix, where the nanoparticles comprise elements selected from the group consisting of gold, silver, titanium, germanium, zinc, aluminum, magnesium, silicon, copper, calcium, iron, barium, potassium, sodium, manganese, nickel, gallium, platinum, and combinations thereof; and the material of the second filament is different from that of the first filament.

The present invention provides a highly active pectinase mutant DS-4 designed based on a deep learning model, as well as its preparation and application. The amino acid sequence of the pectinase mutant DS-4 is shown in SEQ ID NO: 2. Using the deep learning model ProteinMPNN, the pectinase sequence was redesigned to obtain a mutant DS-4 with an enzyme activity more than 2 times higher than that of the wild type. Compared with the WT sequence, DS-4 has a total of 70 amino acid substitutions. This pectinase has broad application prospects in industrial fields such as food processing, textiles, papermaking, and bioenergy.

The application relates to a method for treating dye wastewater by using microalgae to simultaneously collect algal bodies and produce oil, and belongs to the wastewater treatment field, in particular to a method for treating dye wastewater by using microalgae to simultaneously collect algal bodies and produce oil. The method comprises the following steps: using simulated dye wastewater as a culture medium of microalgae, using various substances in the wastewater, such as dyes, to promote the increase of the biomass of the microalgae and the efficient accumulation of oil, reduce the cost of the culture of the microalgae and the production of biological energy, make the dyes be efficiently removed, reduce environmental pollution, and strengthen the sedimentation of the microalgae and reduce the difficulty of the collection of the microalgae. The method realizes low-cost wastewater treatment and biological energy production, and is an ideal method for treating dye wastewater.

This application provides for the medical use of a biophoton generator that can produce bioenergy that promotes health and wellness. Specifically, this application provides a method for needle-free biophoton therapy to optimize meridian energy, used alone or in combination with another therapeutic energy source.

The invention discloses a micromolecule RNA AsrR9 and application thereof in regulation and control of ammonium salt stress tolerance of synechocystis PCC6803, and belongs to the field of industrial microorganisms. According to the invention, AsrR9 is subjected to overexpression, such that an overexpressed algae strain OEAsrR9 is obtained. In a BG11 solid culture medium, the growth state of the OEAsrR9 in the range of 30 mM to 120 mM is obviously superior to that of WT. In a BG11 liquid culture medium added with 90 mM to 150 mM of ammonium salt, illumination culture is carried out for five days, the growth condition of the OEAsrR9 on the third day is obviously superior to that of WT, and the growth condition of the WT is obviously superior to that of an inhibiting algae strain SPAsrR9. The OEAsrR9 obtained by the invention provides a genetic engineering algal strain chassis for constructing algal strains tolerant to various environmental stresses, provides important theoretical support for high-concentration and high-ammonia wastewater treatment and biological energy production by utilizing microalgae in the future, and has practical significance and wide application prospects.

The invention discloses a modular wetland adopting a biological power generation process. Comprising a frame, and a front filter module, a plant filter module and an electrochemical power generation module which are sequentially communicated are arranged in the frame; wherein the front filtering module is used for preliminarily precipitating tail water; the plant filtering module is used for planting high-absorptivity plants of PFASs (perfluoro and polyfluoroalkyl substances), so that the plant filtering module is used for treating the PFASs by plant root systems; and the electrochemical power generation module is used for further processing PFASs and performing biological power generation. According to the invention, a subsurface flow wetland is used as a basis and is matched with an MFC composite technology to integrate the wetland, so that the water quality purification treatment of tail water is enhanced, the utilization of wetland bioelectricity is deepened, biological energy power generation and sewage advanced treatment are realized, and the purposes of industrial greening and system carbon neutralization are achieved with the assistance of conventional sewage plant photovoltaic power generation; the method can be popularized and applied to daily water ecological operation and maintenance work.

The invention provides an interventional brain-computer interface system. The interventional brain-computer interface system comprises a biocompatible packaging shell, and a micro neural signal sensor, a biofuel cell, a low-power-consumption signal processing chip, a wireless communication module and an energy collection and management system which are arranged in the biocompatible packaging shell, the acquisition end of the micro neural signal sensor extends out of the shell to the cerebral cortex to acquire neuron electric signals, and the low-power-consumption signal processing chip processes the neuron electric signals to form digital signals and transmits the digital signals to external equipment through the wireless communication module; the biological fuel cell generates electric energy by utilizing biological energy of a human body; the energy collection and management system is used for collecting and managing electric energy to provide continuous power support for the micro neural signal sensor, the low-power-consumption signal processing chip and the wireless communication module. The problems that an interventional brain-computer interface system is difficult in long-term stable power supply and low in neural signal acquisition precision and transmission efficiency are effectively solved.

The present application relates to a kind of lytic polysaccharide monooxygenase mutant and cellulose synergistic hydrolysis method.The present application belongs to the field of biotechnology, and traditional cellulose hydrolysis technology has the problems of low efficiency and high cost, the present application obtains mutant with higher catalytic activity and stability by site-directed mutagenesis to the 2nd amino acid sequence shown in SEQ ID No:1, significantly improves cellulose hydrolysis efficiency, and has wide application prospect in the fields of bioenergy, papermaking, textile and the like.

A bioenergy generator to increase the life force of a biological system is provided. The bioenergy generator includes fine pieces of natural stone selected from the group of diamond, amethyst, tourmalinejadestone with any color, and obsidian. The bioenergy generator includes fine pieces of natural sand selected from the group of white sand, black sand, pink sand, red sand, yellow sand, orange sand, and green sand. The bioenergy generator includes fine pieces of metal selected from the group of steel, iron, copper, zinc, aluminum, calcium, magnesium, silver, and gold. The bioenergy generator includes water and a supporting material operable to hold the natural sand, the natural stone, the metal, and the water together. Uses of the bioenergy generator are also provided, in particular, to enhance natural healing ability of a biological body and to treat a disease in a mammal.

To provide a high-temperature glucanase mutant having improved alkali resistance, and to provide an application thereof.SOLUTION: The high-temperature glucanase mutant having improved alkali resistance has a specific amino acid sequence. The high-temperature glucanase mutant provided by the present disclosure is obtained by point mutation of a wild-type amino acid of glucanase, and the mutant has a relatively high enzyme activity at neutral and alkaline pH, can withstand high-temperature treatment at 50 °C or higher, and has a high application potential in industries such as hemicellulose degradation, feed, bioenergy, and food.SELECTED DRAWING: Figure 1