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Shewanella haliotis strain and its application in bioelectricity generation

A technology of Boshewanella and bacterial suspension, applied in the field of Boshewanella and its application in generating bioelectricity, can solve the problems such as not obtained, and achieve the effect of reducing requirements and strong electrochemical activity

Active Publication Date: 2013-09-04
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some people think that all Shewanella bacteria can produce electricity in MFC, but only a few Shewanella species have been tested and verified for electricity production, and many newly isolated species have not been more verified.

Method used

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  • Shewanella haliotis strain and its application in bioelectricity generation
  • Shewanella haliotis strain and its application in bioelectricity generation
  • Shewanella haliotis strain and its application in bioelectricity generation

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Experimental program
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Effect test

Embodiment 1

[0029] 1. Screening and isolation of strain CCTCC M 2012444

[0030] Take 300mL of mangrove bottom mud and transfer it to a 500mL beaker, add 200mL of glucose nutrient solution, stir well, and use NaHCO 3 Adjust the pH value of the solution to about 7.0 (6.7-7.2), and place it in a 30°C incubator for 1 day. Measure the pH and, where appropriate, use NaHCO 3 Adjust the pH of the solution to 6.7-7.2, stir evenly, and discard the supernatant after precipitation. Repeat the culture for 3 days for later use.

[0031] To start a single-chamber air cathode microbial fuel cell, the MFC is a cuboid (length×width×height=8.5×7×6.5cm 3 , effective volume 200mL), the material is 0.5cm plexiglass, one end is closed with a blind plate, and the other end is placed with a splint to place the cathode. The air cathode is platinum-loaded carbon paper (0.5mg / m 2 ), the anode is nitric acid modified carbon felt (BET 1200m 2 / g, 2mm), the electrode spacing is 3cm, and titanium wire is used as ...

Embodiment 2

[0037] The strain CCTCC M 2012444 was inoculated into high-salt LB medium, and cultured anaerobically at (30±1)°C for 18 hours. Take the bacterial solution in the mid-logarithmic growth phase, centrifuge at 5000r / min for 10 minutes, discard the supernatant, add PBS buffer and shake well, and centrifuge the obtained bacterial suspension again according to the above steps, repeat washing twice in this way, and add PBS buffer Make bacterial suspension, OD 600 It is about 0.6.

[0038] Start the single-chamber air cathode microbial fuel cell, the microbial fuel cell is cylindrical (cross-sectional diameter is 2cm, effective volume 6.28cm 3 ), made of polycarbonate. The air cathode is platinum-loaded carbon paper (0.5mg cm -2 ), the anode is nitric acid modified carbon felt, the inside of the cathode is a layer of cation exchange membrane, the electrode spacing is 2cm, and titanium wire is used as the electronic current collector. When starting up, take the bacterial solution i...

Embodiment 3

[0042] The strain CCTCC M 2012444 was inoculated into high-salt LB medium, and cultured anaerobically at (30±1)°C for 18 hours. Take the bacterial solution in the mid-logarithmic growth phase, centrifuge at 5000r / min for 10 minutes, discard the supernatant, add PBS buffer and shake well, and centrifuge the obtained bacterial suspension again according to the above steps, repeat washing twice in this way, and add PBS buffer Make bacterial suspension, OD 600 It is about 0.6.

[0043] Start the single-chamber air cathode microbial fuel cell, the MFC is cylindrical (the cross-sectional diameter is 2cm, the effective volume is 6.28cm 3 ), made of polycarbonate. The air cathode is platinum-loaded carbon paper (0.5mg cm -2 ), the anode is nitric acid modified carbon felt, the inside of the cathode is a layer of cation exchange membrane, the electrode spacing is 2cm, and titanium wire is used as the electronic current collector. When starting, mix the bacterial suspension and nutr...

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Abstract

The invention discloses a Shewanella haliotis strain and its application in bioelectricity generation. The Shewanella haliotis strain is Shewanella haliotis Z4, is preserved by China Center for Type Culture Collection called CCTCC for short, has a preservation number of CCTCC NO:M2012444, and is preserved on Nov., 6, 2012. The strain can transmit electrons to an extracellular electron acceptor under an anaerobic condition, and can degrade organic matters and generate electric energy when the strain is inoculated to a microbial fuel cell. The Shewanella haliotis Z4 has a very strong electrochemical activity, and can generate electricity by utilizing a plurality of types of organic matters as a sole carbon source. The Shewanella haliotis Z4 has a very good application prospect in polluted environment restoration and biological energy recovery based on the above characteristics.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollution and bioenergy, and specifically relates to a Boshewella strain and its application in bioelectricity generation. Background technique [0002] In the 21st century, energy shortage and environmental pollution caused by energy development and utilization are two major challenges facing the sustainable development of modern human society. Solving these two problems will be the key to sustainable development. The recycling of sewage seems to be one of the models to achieve recycling while eliminating pollution. [0003] A microbial fuel cell (MFC) is a device that uses microorganisms as a catalyst to oxidize organic matter through its metabolism and convert chemical energy into electrical energy. From the perspective of energy and environmental protection, applying microbial fuel cells to the field of wastewater treatment, applying MFC technology to wastewater...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20H01M8/16C12R1/01
CPCY02E60/50
Inventor 朱能武谢海秀吴平霄
Owner SOUTH CHINA UNIV OF TECH
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