A method for improving the biomass and oil accumulation of oleaginous microalgae using a two-stage culture strategy of admixture and nitrogen-enrichment-nitrogen deficiency

A technology for oil-producing microalgae and biomass, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as synthesis limitation and percentage increase, and achieve the effect of promoting biomass and

Inactive Publication Date: 2011-12-07
JIANGNAN UNIV
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Problems solved by technology

However, when the nitrogen source is lacking, the synthesis of nitrogen-containing compounds such as protein and nucleic acid is limited, while the storage lipid

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  • A method for improving the biomass and oil accumulation of oleaginous microalgae using a two-stage culture strategy of admixture and nitrogen-enrichment-nitrogen deficiency
  • A method for improving the biomass and oil accumulation of oleaginous microalgae using a two-stage culture strategy of admixture and nitrogen-enrichment-nitrogen deficiency
  • A method for improving the biomass and oil accumulation of oleaginous microalgae using a two-stage culture strategy of admixture and nitrogen-enrichment-nitrogen deficiency

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Embodiment 1

[0011] Cultivation of Oleaginous Microalgae Under Mixotrophic Conditions S. dimorphus , algal species from the National Biology Experimental Teaching Center of the School of Life Sciences, Sun Yat-sen University. Under the experimental conditions, a bubbling columnar reactor was used for the test. The effective volume of the reactor was 1.5L. An appropriate amount of algae was inoculated into the BG11 sterilized medium added with glucose. The initial inoculation amount was 0.05g / L and the pH was 7.0. The temperature is 25±1°C, the light is 6600 lux, and the inorganic nitrogen source is NaNO 3 The concentration of glucose is 1.5g / L, the ventilation is 1 L / min, the air light-dark cycle ratio is 14:10, and the added concentration of glucose is 1.2g / L and 5.4g / L respectively. After the microalgae grow to the stable stage, the algae liquid is centrifuged and concentrated, and inoculated with NaNO 3 In a nitrogen-deficient medium with a concentration of 0.3g / L, culture for 14-16 d...

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Abstract

The invention discloses a method for improving biomass and oil accumulation of oleaginous microalgae Scenedesmusdimorphus by adopting a two-stage culture strategy of co-culture and nitrogen deficiency, and belongs to the technical field of microalgae bioenergy. The invention adds a certain concentration of organic carbon source glucose to the autotrophic culture medium, and carries out concurrent culture with CO2 as the autotrophic carbon source. At the same time, under nitrogen-enriched conditions, after high-density culture of microalgae to a stable stage, the algae liquid is transferred to a nitrogen-deficient medium to continue the culture. When the glucose concentration is 1.2-5.4g/L, the initial pH is 7, and the culture time is 14-16 days, the biomass of microalgae can reach up to 4.5g/L, which is 2.57 times the biomass under autotrophic conditions; nitrogen deficiency Under these conditions, the total lipid content of microalgae reaches the highest of 29.62%, which is 1.6 times that of the nitrogen-enriched stage. By adding organic carbon sources and providing nitrogen-deficient conditions for two-stage culture, the biomass of microalgae can be effectively increased and oil accumulation can be promoted, which is of great significance to the research of large-scale production of bioenergy by microalgae.

Description

technical field [0001] Improving Oleaginous Microalgae Using Cocultivation and Nitrogen-enriched-Nitrogen-deficiency Two-Stage Culture Strategies S. dimorphus In the method of biomass and lipid accumulation, the biomass of microalgae can be increased by adding organic carbon source glucose to the BG11 medium, and at the same time, nitrogen deficiency conditions can be provided after the microalgae reaches a stable period, which can effectively increase the total lipid content of the microalgae. The invention belongs to the technical field of microalgae bioenergy. Background technique [0002] Microalgae are a kind of aquatic plants, mainly use light as energy source, and use carbon dioxide as carbon source to carry out photosynthesis to accumulate organic matter. At present, the culture method of microalgae is mainly photoautotrophic culture, which is determined by its own characteristics. However, due to the low cell density of autotrophic culture and the high cost of har...

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 刘和张姗姗范金凤符波张丽娟
Owner JIANGNAN UNIV
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