69 results about "Nitrogen deficiency" patented technology

The invention discloses a method for improving biomass and oil accumulation of oleaginous microalgae Scenedesmusdimorphus by adopting a two-stage culture strategy of co-culture and nitrogen deficiency, and belongs to the technical field of microalgae bioenergy. The invention adds a certain concentration of organic carbon source glucose to the autotrophic culture medium, and carries out concurrent culture with CO2 as the autotrophic carbon source. At the same time, under nitrogen-enriched conditions, after high-density culture of microalgae to a stable stage, the algae liquid is transferred to a nitrogen-deficient medium to continue the culture. When the glucose concentration is 1.2-5.4g/L, the initial pH is 7, and the culture time is 14-16 days, the biomass of microalgae can reach up to 4.5g/L, which is 2.57 times the biomass under autotrophic conditions; nitrogen deficiency Under these conditions, the total lipid content of microalgae reaches the highest of 29.62%, which is 1.6 times that of the nitrogen-enriched stage. By adding organic carbon sources and providing nitrogen-deficient conditions for two-stage culture, the biomass of microalgae can be effectively increased and oil accumulation can be promoted, which is of great significance to the research of large-scale production of bioenergy by microalgae.

A simplified efficient fertilizer specially used for corn and suitable for saline-alkali land and a manufacturing process thereof are disclosed. The fertilizer comprises, by weight, 25-30% of N, 8-12% of P2O5, 4-8% of K2O, 1-4% of humic acid, 0.2-0.5% of Zn, 0.4-0.8% of Ca and 0.4-1.0% of a nitrification inhibitor. The method and the process are advantageous in that 1) added functional factors comprising the humic acid and calcium superphosphate are acidic and can improve soil alkalinity, and calcium ions of the calcium superphosphate can exchange sodium ions in soil so as to obviously reduce soil salinity, 2) urea-formaldehyde added in a granulation step can obviously improve the granulation rate and granular strength of the fertilizer, thus overcoming a later fertilizer granule efflorescence problem, slow-release nitrogen in the urea-formaldehyde and quickly available nitrogen in urea are combined, thus meeting a corn growth absorption curve and avoiding later nitrogen deficiency for corn, and addition of the nitrification inhibitor reduces nitrogen loss and increases the nutrient utilization rate, and 3) an anti-blocking agent used in the fertilizer is synthesized through saponification, is low in cost, adopts waste grease, is green and meets the strategy of sustainable development.

The invention discloses application of small RNA miR169 or target genes NFYA5 thereof related to nitrogen metabolism in the nitrogen stress adaptation of plants, which is characterized in that miR169 is excessively expressed in Arabidopsis thaliana (L.) Heynh to increase the sensitivity of the Arabidopsis thaliana (L.) Heynh on nitrogen deficiency stress; and the target genes NFYA5 of miR169 is correspondingly excessively expressed to reduce the sensitivity of the Arabidopsis thaliana (L.) Heynh on the nitrogen deficiency stress and enhance the capability of the Arabidopsis thaliana (L.) Heynh on low nitrogen stress resistance. The invention can ensure that the analysis and the deeply knowledge of the complicated regulate and control network can be further applied to the cultivation of crops of high-efficiency nitrogen.

The invention discloses a high-spectrum-based rapid nondestructive high-accuracy method for identifying the abundance degree of a nitrogen element in a plant leaf. The method comprises the following steps: performing high-spectral collection on a leaf sample; performing noise-reduced preprocessing on high-spectral data and then performing characteristic extraction on the spectral data; establishing a correction model of the spectrum characteristic and the category of the level standard of the nitrogen element in the plant leaf; collecting high-spectral data of an unknown sample; performing the noise-reduced preprocessing on the spectral data of the unknown sample and then converting a spectrum of the unknown sample to form a characteristic space; substituting the correction model into the characteristic space so as to perform identifying on the level category of the nitrogen element, so as to obtain whether the plant leaf is in nitrogen deficiency or contains the nitrogen element with the normal/excessive amount. According to the technical scheme, a portable high-spectral instrument is utilized to collect the spectrum of the plant leaf; an Adaboost+SLPP characteristic extraction method is utilized to perform the characteristic extraction on the spectral data of the plant leaf; and a KNN (K-Nearest Neighbor) serves as a categorizer, so that the identification predicting accuracy is improved effectively.

The invention discloses a method for producing medium-chain-length polyhydroxyalkanoates by virtue of excess activated sludge, and relates to polyhydroxyalkanoates. The method comprises the following steps: using sodium laurate as a unique substrate carbon source for domesticating original sludge, and synthesising poly-3-hydroxybutyrate-co-3-hydroxyhexanoate by virtue of the stably-domesticated activated sludge, wherein 1.5g/L sodium laurate is uniformly poured for three times while being used as the unique substrate, and the highest concentration of cumulative PHBHHx of the sludge is 505.5mg/L under a continuous aeration condition and in case of nitrogen deficiency and phosphorus deficiency; the PHBHHx are synthesised by virtue of activated sludge mixed micro-organisms for the first time, and an infrared absorption spectrum and thermogravimetric analysis proves that PHBHHx samples have chemical structures and physical properties similar to those of PHBHHx standard substances synthesised from pure strains. The method is simple in equipment, easy to operate, free from a sterile working condition and rich in excess sludge source, and therefore has a great application prospect in the aspect of producing plastic products needed in the industry and the agriculture.

The invention relates to a microalgae cultivation method and a grease production method. The cultivation method comprises the following steps: a nitrogen fertilizer is provided for microalgae for many times; at least for one time, the nitrogen fertilizer is added when the nitrogen fertilizer in a microalgae suspension is consumed by microalgae until the microalgae is in a nitrogen-deficiency simulation state; and the cultivation time lasts for more than 10 days. By the cultivation method, normal growth of microalgae can be guaranteed, and the microalgae can accumulate much grease.

The invention discloses a gene GmSPX5 for regulating and controlling growth of leguminosae root nodules, and the application of gene GmSPX5. The nucleotide sequence of the gene is as shown in SEQ ID NO.1 and the coding amino acid sequence is as shown in SEQ ID NO.2. The research shows that excessive expression of the GmSPX5 can increase the number of the leguminosae root nodules and the density ofroot nodules infecting cells under high phosphorus treatment; furthermore, the nitrogen phosphorus content and the yield of soybeans can be remarkably increased. Therefore, the GmSPX5 can regulae andcontrol the growth and development of the root nodules and has important effects of improving the nitrogen phosphorus nitration of plants and increasing the yield; and the gene GmSPX5 can be used forregulating and controlling the adaptability on phosphorus deficiency and nitrogen deficiency in soil by the plants through a transgenic technology, also can be applied to leguminous crop nitrogen phosphorus synergistic and efficient genetic improvement and has a very important market prospect.

The invention discloses an application of a gene FERONIA, and belongs to the technical field of gene regulation and control. The invention discloses a method for adding amino acid into a nitrogen-deficient culture medium. Wild type arabidopsis thaliana Col-0 and FERONIA gene deletion mutants fer-4 are dibbled on a culture medium of the arabidopsis thaliana; amino acid capable of restoring the nitrogen deficiency phenotype of wild arabidopsis thaliana is found, but the nitrogen deficiency phenotype of the arabidopsis thaliana fer-4 mutant cannot be restored or only part of the amino acid can berestored, and it is shown that FERONIA participates in the utilization process of organic nitrogen by plants, that is, the FERONIA gene of arabidopsis thaliana participates in the response process ofplant roots. The invention provides a theoretical and method basis for functional research of the FERONIA gene. The FERONIA disclosed by the invention can provide theoretical basis and genetic resources for cultivating new crop varieties.

The invention relates to a method for improving yield of heterotrophic microalgal fatty acid with sodium acetate. The method comprises the following steps: a) performing amplified culture on microalage in an autotrophic culture medium to obtain sufficient algae; b) inoculating the algae in the step a) into a nitrogen-deficiency heterotrophic culture medium; and c) performing nitrogen-deficiency heterotrophic culture.

The invention discloses a method for promoting single-needle algae accumulated grease by nitrogen deficiency combined with strigolactones. Single-needle algae is cultivated through two stages. The method for promoting the single-needle algae accumulated grease by the nitrogen deficiency combined with the strigolactones comprises the following steps that firstly glucose is used as an organic carbonsource to heterotrophically cultivate the single-needle algae to a logarithmic phase later period to collect algae cells; then autotrophy anazotic BG-11 is used as an inducing culture medium of a second stage to renewedly suspend the algae cells to be used as inducing algae liquid; 1mmol/L strigolactones (SLs) mother solutions are prepared by acetone, the strigolactones (SLs) mother solutions areadded into the inducing algae liquid to dilute the strigolactones (SLs) mother solutions to the concentration of 1[mu]mol/L, and the algae liquid is placed in a shaking table for lighting cultivating; and the algae cells cultivated to a stable phase are enriched by a centrifugal separation method, grease is extracted, and methyl esterification is carried out to prepare biodiesel. The method for promoting the single-needle algae accumulated grease by the nitrogen deficiency combined with the strigolactones is simple and practicable, can greatly shorten the cultivated time of the oil-producingsingle-needle algae, obviously increases the grease yield, and thus, greatly improves the efficiency of microalgae for producing the biodiesel.

The invention relates to a method for recommending a nitrogen fertilizer for wheat according to a relative SPAD (Soil and Plant Analyzer Development) value of a plant leaf. The method comprises the following steps: 1) setting field trial treatment (cells) of different nitrogen application levels, so as to cover gradient levels of soil nitrogen supply from nitrogen deficiency to excessive nitrogen supply; 2) testing the yield of wheat of soil under different nitrogen supply levels, and calculating an optimal soil nitrogen supply amount with the maximum wheat yield according to the relationship of the wheat yield and the soil nitrogen supply amount; 3) testing leaf SPAD values under different soil nitrogen supply conditions, calculating the relative SPAD value, and establishing a relationship model of the relative SPAD value and the soil nitrogen supply; and 4) testing the SPAD value of wheat to be supplied with the nitrogen fertilizer, calculating a relative SPAD value, and confirming the nitrogen fertilizer recommending amount according to the optimal soil nitrogen supply amount and the relationship model of the relative SPAD value and the soil nitrogen supply amount. By adopting the method provided by the invention, dynamic demands of the crop on a nitrogen nutrient component can be precisely matched, so that the yield of the crop can be increased, and the nitrogen fertilizer utilization efficiency can be improved.

The invention discloses a cultural method of obtaining heterotrophic chlorella cells with a high protein content. The operating method comprises the following steps of: heterotrophically culturing chlorella to an initial period of a growing logarithmic phase by using a nitrogen deficiency modified BG11+ organic culture medium; centrifugally collecting the algae cells in a sterile condition; and transferring the algae cells to a nitrogen sufficiency modified BG11+ organic culture medium to be continuously cultured to an initial period of a stable period to collect the algae with the high protein content. On the premise of guaranteeing the heterotrophic high biomass of the chlorella, the method improves the protein content in the heterotrophic chlorella living bodies, so that the output of the proteins is improved; and moreover, the produced single-cell proteins are reasonable in biochemical composition, and amino acid components meet the nutritional demand on foods and feeds. The method disclosed by the invention provides a novel technical route for improving the output of the single-cell proteins. The process has the advantages of being simple in process, simple to operate, short in production period, high in product quality and the like, and is easy for scaled production.

The invention relates to a strategy for growth and metabolism regulation of microalgae cultured by urban sewage. The strategy mainly includes: (1) in an early culture stage, a plant growth regulator and COD (chemical oxygen demand) degrading bacteria are added into microalgae-containing urban sewage to promote chlorophyll synthesis and degradation of organics in sewage and improve microalgae cellphotosynthetic efficiency and carbon sequestration efficiency to increase biomass yield; (2) in a later culture stage, the nitrogen content of sewage meets a nitrogen deficiency condition, and accordingly a glycolytic pathway is regulated so as to promote carbohydrate metabolism to enable more carbon for fatty acid synthesis, and grease accumulation promotion is realized. Adopting the urban sewagefor culturing microalgae meets the requirement of a two-stage culture process in a same system, both microalgae high-density culture and grease accumulation can be achieved, simplicity and convenience in operation are achieved, and large-scale production is facilitated; the strategy is significant to guidance of large-scale microalgae culture and provides theoretical and technical support for process optimization of an industrial chain for preparing biodiesel from microalgae cultured by urban sewage.

The invention discloses a method for excavating and selecting anabolic markers of microalgae oil. In a nitrogen deficiency induction condition, three kinds of algae with extremely poor affinis, including freshwater chlorella, seawater nannochloropsis oculata, and polar coccomyxaceae, are used as subjects, and based on cytomics, metabonomics, andbioinformatics methods, a same anabolic marker synthesized and accumulated by the three kinds of algae cell oil with extremely poor affinis is excavated and selected. Using complementary analysis among different algaes and different omics, a result one-sidedness caused by using a single algae and a single analysis method can be effectively prevented, so as to greatly increase selection identification rate of a key anabolic marker synthesized and accumulated by the microalgae oil and corresponding precision. The method provides theoretical basis for further improving yield of the microalgae oil and revealing an anabolism general mechanism of themicroalgae oil.

The inventiondiscloses a production method of a semi-sweet liqueur type lycium barbarumfruit wine, the semi-sweet liqueur type lycium barbarumfruit wine is prepared by taking fresh lycium barbarum asthe raw material and conducting fermenting and brewing for two times, the anaerobic treatment is conducted on the fresh lycium barbarum in the method, and then the brewing technique combining the nitrogen-deficiency incomplete fermentation and the lactobacillus fermentation is used to brew the wine. The semi-sweet liqueur type lycium barbarumfruit wine produced by using the method is good in tasteand rich in nutrients, and further has the effect of oxidation resistance, the anti-cancer effect and the like. The production method of the semi-sweet liqueur type lycium barbarumfruit wine has theadvantages of simple process, easy operation and the like, can be put into production directly in the common fruit wine brewery, and does not need extra investment to buy equipment.

The invention belongs to the technical field of plant gene engineering. A phosphorus deficiency inducible expression promoter is cloned from Brassica napus, and has a nucleotide sequence shown as 1-1459th base group in a SEQ ID NO.1 in the sequence table; the promoter is fused with a report gene GUS and transferred into Arabidopsis; and GUS histochemical analysis shows that the promoter only has activity under phosphorus deficiency conditions, and is lack of activity under normal, nitrogen deficiency, potassium deficiency, sulfur deficiency and iron deficiency conditions. In addition to application to cultivation of low phosphorus tolerant or phosphorus efficiency tolerant crop, the promoter can also be applied to research on regulation mechanism of phosphorus deficiency induced expression gene.

The invention belongs to the technical field of organic fertilizers, and particularly relates to a straw decomposed organic fertilizer and a preparation method thereof. The straw decomposed organic fertilizer is prepared from the following components in parts by weight: 200-400 parts of crop straw, 50-80 parts of vegetable leaves, 20-40 parts of animal waste and 0.5-1.0 part of a composite microbial decomposition agent; wherein the composite microbial decomposition agent comprises pseudomonas stutzeri, penicillium oxalicum, rhizopus oryzae, trametes versicolor and white rot fungi. Crop straw, vegetable leaves and animal waste are mixed in proportion to serve as an organic material, the carbon-nitrogen ratio of a single straw organic matter can be reduced, and the situation of nitrogen deficiency after the fertilizer is applied to soil is avoided. By adding the composite microbial decomposition agent, the time required for decomposition can be greatly shortened, the reduction speed of the carbon-nitrogen ratio is increased, preservation of nitrogen, phosphorus and potassium in compost and increase of organic matters are facilitated, and nutrient balance is promoted. After the fertilizer is applied to soil, the content of organic matters and available nutrients in the soil can be increased, acidified soil is improved, and soil fertility is increased.

The invention belongs to the technical field of plant genetic engineering, and specifically relates to a nitrogen supply recovery specific induced expression promoter Y2 after paddy rice nitrogen deficiency, and an application thereof. With a chip technology, promoter Y2 downstream gene nitrogen supply induced expression information is obtained. With different paddy rice varieties, Y2 downstream gene nitrogen supply induced expression mode is verified. With a PCR method, the promoter Y2 full length and a 5'-terminal truncated fragment thereof are amplified, and are connected to a promoter vector DX2181b, such that a promoter fusion GUS expression vector is obtained. The constructed vector is used in genetic transformation upon a paddy rice variety Zhonghua11. The regulation of the promoter upon GUS gene is verified in a transformation positive plant. Through Realtime expression level verification and GUS protein activity detection, it is further determined that the promoter is a nitrogen supply recovery specific induced expression promoter after nitrogen deficiency. A functional section is at AT6 upstream 519bp.

The invention relates to the technical field of soil improvement, in particular to a soil improvement method. According to the method, an organic material with a reasonable proportion is prepared, a microbial agent capable of accelerating the fermentation speed is added, the soil is irrigated until the maximum field water holding capacity reaches 80%, then the soil is covered with a film, one timeof watering is carried out at the periphery of the film at the 5 day and the 10 day separately after film application, so that a gap between the edge of the periphery of the film and the soilis sealed by water, and the problems of conventional RSD treatment at a lower temperature can be solved that the treatment time is long and needs 5-8 weeks, and the treated soil easily has nitrogen deficiency and maintains an anaerobic environment difficultly. After the soil improvement method is used, the pH value of the soil is increased, the unit weight of the soil is decreased, the soil porosity is improved, the content of various nutritional ingredients is obviously increased, the improvement effect of the method is better than that of the conventional RSD treatment, the treatment time is short, and the soil improvement can be completed in only 14-26 days.

The present invention relates to a method for promoting accumulation of microalgae oil under high-light and nitrogen-deficiency stress by using melatonin, and belongs to the field of microalgae biotechnology. A BG-11 basic medium using 10 g/L of glucose as a carbon source conducts heterotrophic culture of monoraphidium sp.QLY-1 at a temperature condition of 24-26 DEG C, algae cells are collected until the monoraphidium sp.QLY-1 grows to a late logarithmic phase, and a nitrogen-deficiency BG-11 culture medium dilutes the resuspended algae cells to 0.7 g/L as an inducing algae solution; absoluteethanol is used to prepare 10 mmol/L of a melatonin mother solution, the melatonin mother solution is added to the inducing algae solution to dilute a concentration of the melatonin into 10-80 [mu]mol/L, and the solution is subjected to induced culture at a temperature of 24-26 DEG C and an illumination intensity of 100-120 [mu]mol m<-2> s<-1>; and oil in the algae cells is extracted with an organic solvent. The method is simple in operation, can shorten induction cycle of the algae cells, increases content of oil, ensures growth of the microalgae, and can solve problems of biomass reduction,low oil content, etc. in an induction process of microalgae industrialization.

The invention especially relates to a nitrogen-supplementing embedded growth-promoting agent, and a preparation method and application thereof, belonging to the technical field of ecological and environmental protection. The nitrogen-supplementing embedded growth-promoting agent is porous particles and comprises an embedding medium skeleton, and nitrogen-source nutritional components and antioxidation components embedded in the embedding medium skeleton. The nitrogen-supplementing embedded growth-promoting agent has a highly-prosperous pore system and a great pore size; the mass transfer resistance of the nutritional components in pores is small and the nutritional components are utilized by plants and microbes after slow release in a wetland environment; so the loss of the nutritional components is reduced, the utilization rate of the growth-promoting agent is high in the process of plant and microbial restoration, and the growth-promoting agent is applied to cooperative plant and microbial restoration in river mouths or coastal wetland lacking in nitrogen. The preparation method for the nitrogen-supplementing embedded growth-promoting agent provided by the invention is simple and easily practicable; the prepared embedded growth-promoting agent has controllable uniform particle size and excellent performance; industrial scaling-up preparation of the agent can be realized; and the embedded growth-promoting agent has good application prospects in fields like marine ecological engineering and environmental protection.

The invention discloses a method of subjecting Haematococcus pluvialis to attachment culture with a rotary biomembrane reactor to produce astaxanthin; the method comprises the steps of 1, preparing a seed liquid; 2, performing attachment culture; 3, accumulating biomass, to be specific, upon reaching a stable state, using BG11 culture liquid containing 2.1 g/L of sodium nitrate to replace the culture liquid in the rotary biomembrane reactor, and performing batch or supplemented-batch culture; 4, inducing astaxanthin, to be specific, inducing Haematococcus pluvialis to accumulate astaxanthin by means of nitrogen deficiency stress or/and high light density stress, namely replacing the culture liquid with BG11 culture liquid with no sodium nitrate or/and increasing light intensity to 4500-10500 lx; 5, extracting astaxanthin. The advantage that Haematococcus pluvialis in the rotary biomembrane reactor grows fast in attachment manner is given to full play, biomass with high concentration can be acquired, astaxanthin production efficiency is improve, and harvesting cost is lowered.

The invention belongs to the field of a plant genetic engineering technology and specifically relates to a promoter Y8A for specific induced expression by recovering nitrogen supply after nitrogen deficiency of rice. By a chip technology, nitrogen supply induced expression information of downstream gene of the promoter Y8A is obtained, and a nitrogen supply induced expression pattern of the downstream gene of the promoter Y8A is verified through different rice varieties. Through a PCR method, overall length of the promoter Y8A and its 5' end truncated fragment Y8B are amplified and linked to a vector DX2181b so as to construct a promoter-fused GUS expression vector. Then, the constructed vector is transformed into the rice variety Zhonghua 11. In positive transformed plants, regulation of GUS gene expression by the promoter is verified. Through expression quantity verification of Realtime, it is further confirmed that Y8A and Y8B belong to the promoters for specific induced expression by recovering nitrogen supply after nitrogen deficiency and a functional fragment of the promoter is at the position of ATG upstream 982bp. The invention provides a new promoter resource for rice.