Culture method of autotrophic microorganism for producing poly (beta-hydroxybutyrate)

Through the nitrogen-sufficient-nitrogen-deficient two-stage culture method, carbon dioxide is used as the carbon source to produce poly (β-hydroxybutyrate), which solves the problems of complex genetic modification and harsh fermentation process in existing technologies, and achieves greenhouse gas emission reduction and the production of high-value-added bioplastics.

CN120591173AActive Publication Date: 2025-09-05PEKING UNIV SHENZHEN GRADUATE SCHOOL
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Patent Information

Application Number
CN202511099771.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-08-07
Publication Date
2025-09-05
Estimated Expiration
2045-08-07

AI Technical Summary

Technical Problem

The existing technology has problems in the construction of fermentation genetically engineered bacteria, such as complex genetic modification, harsh fermentation process conditions, and high operating energy consumption, making it difficult to economically and effectively use carbon dioxide as a carbon source for the production of poly-β-hydroxybutyrate.

Method used

A two-stage nitrogen-sufficient and nitrogen-deficient culture method was used. Hydrogen-oxidizing bacteria (Rhodoblastus sp. TH20) were first enriched in a nitrogen-sufficient culture medium, and then synthetic culture was carried out in a nitrogen-deficient culture medium to produce poly-β-hydroxybutyrate using carbon dioxide as the carbon source.

Benefits of technology

It achieves greenhouse gas emission reduction and the production of high value-added bioplastics, which has both ecological and economic benefits. The produced biopolyester has good biodegradability.

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Abstract

The invention relates to the technical field of microorganism culture, in particular to a culture method of autotrophic microorganisms for producing poly (beta-hydroxybutyrate). According to the method, carbon dioxide which is wide in source and low in obtaining cost is used as a carbon source for producing poly-beta-hydroxybutyrate (PHB), through a two-stage culture method of nitrogen sufficiency and nitrogen deficiency, firstly, hydrogen oxidizing bacteria (especially Rhodoblastus sp. TH20) are subjected to biomass enrichment, then intracellular biopolyester PHB accumulation is carried out, the obtained biopolyester has good biodegradability, and the biopolyester can be used for producing the poly-beta-hydroxybutyrate (PHB). The greenhouse gas emission reduction can be realized, the bioplastic product with high additional value can be produced, and the method has both ecological and economic benefits.
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