530results about How to "Promote rapid proliferation" patented technology

This invention relates to a method for manufacturing bioorganic fertilizer. The bioorganic fertilizer is manufactured by: mixing sugar industry waste, breeding industry waste, oil-processing waste, phosphoric fertilizer, trace element fertilizer and silicon fertilizer, and performing aerobic fermentation and post fermentation. The effective components of the bioorganic fertilizer are: organic matters (greater than or equal to 30%), N, P and K (greater than or equal to 8%), beneficial microorganisms (greater than or equal to 200,000,000/g), active silicone (greater than or equal to 5%), magnetic components (0.25%), and Fe, Zn and Mo (0.5-1%). The bioorganic fertilizer has abundant raw material sources, and is environmentally friendly.

A process and system used for reporting and processing incidents of aggressive, dangerous, and discourteous drivers using Short Message Service (SMS) data, also known as “text messaging” using key words or characters. A hand held electronic device is used to send a text message location reporting emergency or non-emergency information to a central processing unit. By establishing a process and system that utilizes mobile hand held electronic devices or cell phones to report emergency and non-emergency information through text messaging and downloading that information to the central processing unit, this data is then put through a filtration process to allow the most serious road violators and those needing help to be easily retrieved. The information is then transmitted to a subscriber via text message and/or email for corrective action or for dispatching emergency help.

The invention discloses a tissue culture method of ginseng adventitious root, comprising the steps of induction, amplification and successive transfer culture of ginseng callus; the induced callus is inoculated on the prepared solid culture medium and added with plant growth regulator to be cultured for 3-4 weeks under the conditions of sterility, the temperature of 23-25 DEG C and keeping in dark place; the amplified callus is inoculated on the prepared solid culture medium and added with proper plant growth regulator to be cultured for 3-5 weeks under the conditions of sterility, the temperature of 23-25 DEG C and keeping in dark place, and then the adventitious root can be formed; the formed adventitious root is picked out and inoculated on the prepared liquid culture medium and added with the plant growth regulator to be cultured for 4-5 weeks under the conditions of sterility, room temperature and sunshine. The method has higher actual significance than ginseng cell cultivation, and the growth speed of the adventitious root is much higher than that of the original root; furthermore, the production rate of active secondary metabolite of the adventitious root is high and stabler and is not influenced by natural environment such as natural disaster and the like; the tissue culture method also has the characteristics of simple technique process, high inductivity, good repeatability, rapid propagation of the adventitious root, etc.

The invention relates to a separation method and a culture method for umbilical cord mesenchymal stem cells. The separation method comprises the following steps: thoroughly cleaning umbilical cord tissue of a healthy newborn by using a PBS (phosphate buffer solution) containing streptomycin and penicillin, and removing blood; shearing the umbilical cord into small sections uniform in length, and mechanically separating, bluntly stripping Wharton' s jelly, and removing umbilical arteries and umbilical veins; uniformly shearing the Wharton' s jelly; re-suspending the sheared Wharton' s jelly through an MSCs (mesenchymal stem cells) culture medium, inoculating to a culture dish with laid gelatin, and putting in a CO2 culture box for cultivation; conducting centrifugal separation to obtain tissue blocks and a cell resuspension solution. The culture method comprises the following steps: enwrapping the culture dish, discarding the gelatin, and washing with the PBS; inoculating the separated out tissue blocks and the cell resuspension into the culture dish; performing digestive subculture after cell fusion growth rate reaches 80-90%.

The invention discloses a porcine pseudorabies virus strain as well as an inactivated vaccine and applications thereof, belonging to the field of separation and application of the porcine pseudorabies virus strain. The invention firstly provides a porcine pseudorabies virus BJ strain separated from diseased pig tissues, and the microbial preservation number of the porcine pseudorabies virus BJ strain is CGMCC (China General Microbiological Culture Collection Center) No.7351. The invention discloses a method for preparing the inactivated vaccine by applying the porcine pseudorabies virus BJ strain. The method comprises the steps of culturing a virus strain to obtain a virus solution; adding an inactivator, and inactivating and concentrating the virus solution; and evenly mixing an adjuvant and the virus solution, and emulsifying to obtain the inactivated vaccine. The technological parameters of the inactivated vaccine preparation method are further optimized, and the immune protection efficacy and safety of the inactivated vaccine can be improved. Shown by the immune protection efficacy and safety tests, the porcine pseudorabies inactivated vaccine prepared has good immune protection efficacy and safety, and can be clinically used for preventing or treating porcine pseudorabies.

The invention discloses a composite microbial deodorant and a preparation method thereof. Each liter of the deodorant comprises the following bacteria: photosynthetic bacterium with a viable count of 1.3-7.5*108 CFU, bacillus with a viable count of 0.9-7.8*108 CFU, saccharomycete with a viable count of 1.02-1.92*108 CFU, nitro-bacterium with a viable count of 1-10*108 CFU, thiobacillus with a viable count of 1-6*108 CFU, melanomyces with a viable count of 4.8-13.5*108 CFU, and aspergillus flavus with a viable count of 4.8-11.7*108 CFU. The bacteria mentioned above are basically obtained from garbage, and domesticated by garbage leachate. The deodorant can act on the garbage and garbage leachate, and thus cut off the odor source. The beneficial bacterial colonies can decompose and degrade odorous pollutants in garbage, eliminate the garbage odors, and generate a great amount of beneficial metabolic products and enzymes.

The invention provides an earthworm bioreactor and an organic waste treatment method. The earthworm bioreactor comprises a reactor box body, an organic waste distributing device, an earthworm dung output device, a forced ventilation system, an expansion bracket, an illuminator and an external central regulation and control system, wherein the expansion bracket is connected above the reactor box body; the illuminator is arranged above the reactor box body; distributing wheel tracks which are connected with the organic waste distributing device in a sliding manner are connected to upper edges on both sides of the reactor box body; the earthworm dung output wheel track which is connected with the earthworm dung output device in a sliding manner is arranged inside the reactor box body, and isfixed on the reactor box body; the forced ventilation system is connected on the outer side wall of the reactor box body; a heating interlayer is arranged below the bottom of the reactor box body; and the organic waste distributing device, the earthworm dung output device, the forced ventilation system, the illuminator and a radiating device are connected with the external central regulation and control system. By the earthworm bioreactor, earthworms can be propagated quickly while organic wastes are treated to a great limit.

The invention discloses a microbial preparation which comprises pseudomonas stutzeri, bacillus subtilis, lactobacillus, alcaligenes faecalis and aspergillus niger. The invention further discloses a preparation method of the microbial preparation and a method of strengthening the biochemical water treatment effects by the microbial preparation. The pseudomonas stutzeri, the bacillus subtilis, the lactobacillus, the alcaligenes faecalis and the aspergillus niger in the microbial preparation can form dominant bacterial communities in a sewage treatment system, strengthen the treatment effects of COD (chemical oxygen demand) and the like in sewage, and particularly can form advantages in a printing and dyeing sewage treatment system after matching with aniline-degrading bacteria and aerobic denitrifying bacteria, so that printing and dyeing sewage COD, aniline, chroma and total nitrogen are rapidly degraded in a targeted manner, and the printing and dyeing sewage treatment effects are strengthened.

The invention discloses a method for building a high-efficiency regeneration system of superior corn self-bred line agriculture line 531, belonging to the field of plant genetic engineering and transgenosis breeding. The invention takes an agriculture line 531 rataria as an explant, induces in a callus induction medium and produces an II-type embryonic callus; the II-type embryonic callus is subjected to embryoid induction under light in an embryoid induction medium to produce a green embryoid; then, the green embryoid is transported to a regeneration medium and is cultured into a regeneration plant under light; root induction is carried out in a rooting medium, and acclimatization is carried out in Hogland nutrient solution to ensure that a new thick root grows on the root of the regeneration plant; the root is transplanted to nutritional soil for rejuvenation culture; and finally, the root is transplanted to a land for growing field crops to normally grow and seed. The regeneration technology is suitable for the superior corn self-bred line agriculture line 531 with high application value, can ensure that the superior quality of agriculture line 531 corn can be inherited in the corn transgenosis breeding process, and has an important meaning for functional genome group research.

The invention discloses a method for simultaneously inducing and amplifying V alpha<24+>iNKT cells and CD<3->CD<56+>NK cells. The method comprises the steps as follows: PBMC (peripheral blood mononuclear cells) are separated from peripheral blood, the concentration of the PBMC is adjusted to 2*10<6>/ml by a serum-free medium containing autologous plasma; an Anti-CD<16> antibody, -GalCer, IL-2, IL-18 and IL-21 are added, and then the mixture is transferred into a culture flask for culture; an Anti-CD3 antibody is added in a cell suspension in 24 hours; a serum-free medium containing IL-2, IL-18 and IL-21 is supplemented every two days according to the cell growth condition; the cell concentration is controlled to be 1.5*10<6>/ml; and after continuous culture is performed for 14-21 days, large quantities of high-purity V alpha<24+>iNKT cells and CD<3->CD<56+>NK cells can be obtained simultaneously, and the total cell quantity can reach an effective value of the cell quantity required for adoptive cellular immunotherapy clinically for tumor. The method for simultaneous and efficient amplification of the V alpha<24+>iNKT cells and the CD<3->CD<56+>NK cells is simple, convenient and effective.

Plant-growth-promoting bio-organic fertilizer is prepared by inoculating 0.5-2% (w/w) of composite fermentation microbial agent to livestock-poultry manure and auxiliary material, fermenting, inoculating 2-5% (w/w) of plant-growth-promoting microbial agent and fermenting. A preparation method includes steps: (1), preparing a fermenting material; (2), performing high-temperature aerobic fermentation; (3), preparing the plant-growth-promoting microbial agent; (4), after-composting organic fertilizer. The plant-growth-promoting bio-organic fertilizer and the preparation method have the advantages that rapid proliferation of plant-growth-promoting functional microorganisms is realized in the process of fermenting of two times, and effective biological viable bacteria count of the organic fertilizer reaches 5x108CFU/g; composting degree is high, the organic fertilizer can generate various plant hormones including 3-indoleacetic acid, cytokinin, gibberellins and ethylene after being applied in soil, can promote plant growth and can increase yield of crops and incoming of farmers; the bio-organic fertilizer prepared by the method is low in cost, environment-friendly and high in efficiency.

The invention discloses a method and a special culture medium for subculturing chicken embryonic stem cells for a long time. The method is characterized by comprising the following steps of: isolating cells of area pellucida of X-stage blastoderm, dispersing into single cells or small cell masses through mechanical blowing and beating, inoculating on an STO feeder layer, culturing the chicken embryonic stem cells in the special culture medium at the temperature of between 37 and 38 DEG C, and subculturing once every 3 to 5 days, wherein the special culture medium comprises 600 to 900mL of conditioned medium, 50 to 150mL of fetal calf serum, 5 to 20mL of chicken serum, 5 to 25mL of one or a mixture of more of non-essential amino acids, 0.146 to 0.292g of L-glutamine, 6 to 14mu L of beta-mercaptoethanol, 1 to 2*10<6> IU of mouse leukemia inhibitory factor, 10 to 50mu g of alkaline fibroblastic growth factor and 5 to 20mu g of stem cell growth factor; and fixing the volume of the ingredients to 1,000mL by using a dulbecco's modified eagle medium (DMEM) (high glucose), and regulating the pH value to 7.2 to 7.5. The conditioned medium is obtained by the steps of: culturing BRL-3A cells until the cells are converged, collecting supernatant of the cultured cells, performing centrifugal separation, and filtering by using a filter membrane to obtain the conditioned medium. Compared with the prior art, the method has the advantages that: the long-term subculture of the chicken embryonic stem cells can be realized, the proliferation of the cells is quick, and the cloning yield is high.

The invention relates to a biologic polypeptide medicine blood vessel support and a preparation method thereof. The biologic polypeptide medicine blood vessel support comprises a support body and active medicines. The support body which is medical material with pores and good biocompatibility is made from stainless steel, cobalt-base alloy, titanium alloy, nickel-titanium alloy or polylactic acid biopolymer; the active medicines comprise a biologic polypeptide medicine and a smooth muscle cell proliferation inhibition medicine. The support is characterized in that: the support body with the pores has the biologic polypeptide medicine fixed on the internal surface of the body and the smooth muscle cell proliferation inhibition medicine coated on the external surface of the body. The preparation me(3) carrying out the after-treatment of the surface of the support body; (4) preparing the medicines; (5) coating the external surface; (6) fixing the medicine onto the internal surface; (7) carrying out the process step of low temperature drying. The support can selectively absorb endothelium progenitor cells which quickly differentiate into endothelium cells to promote the restoration of the endothelium after the support is built in; the support can also effectively inhibit the proliferation and migration of vascular smooth muscle cells, persistently and effectively reduce the formation of a new inner membrane, effectively prevent restenosis inside the support, and avoid the risk of potentially fatal late thrombosis.

The invention relates to an organic-inorganic composite gel material for bone repair and a preparation method thereof. The composite gel material is characterized in that the organic material is formed by synthetic polymers or natural polymers; the inorganic material is formed by SiO2, CaO and P2O5 with mole fraction of 50-80%, 10-40% and 1-10% respectively; and the mass ratio of the organic material to the inorganic material is (0.1-1):1. The method is characterized by penetrating the polymer chains with biocompatibility and biodegradability into the SiO2 inorganic network structure to form the semi-interpenetrating structure. In the invention, the good blending of the organic and inorganic components at molecular level is realized, the mechanical property of the materials is simultaneously improved, and the bioactivity of the materials is improved; and the material and preparation method have important development potential in the field of bone defect repair.

The invention relates to the technical field of tissue engineering scaffolds, and in particular relates to a preparation method of a cartilage repair scaffold material. The preparation method comprises the following steps: firstly, preparing chitosan nano fibers containing hydroxyapatite by virtue of electrostatic spinning; then, packing by using hyaluronic acid; finally, preparing the cartilage repair scaffold material by virtue of a freeze-drying experimental method. The cartilage repair scaffold material obtained by the preparation method disclosed by the invention has a relatively large specific area and is favorable for cartilage cells to grow and proliferate; hydroxyapatite serves as the main component in a human bone to provide a lot of necessary raw materials for the growth of the cartilage cells; hyaluronic acid is the main component of cartilage liquid and is favorable for providing a good growth environment for the cartilage cells; meanwhile, the hyaluronic acid can be compounded with chitosan to package nano hydroxyapatite in chitosan so as to form a sustained-release system of hydroxyapatite; growth factors can be used for promoting rapid proliferation of the cartilage cells.

The invention discloses a composite enrichment medium for salmonella, staphylococcus aureus, escherichia coli, listeria monocytogenes and vibrio parahaemolyticus and a preparation method for the composite enrichment medium. The composite enrichment medium comprises the following components in parts by weight: 5-10 parts of ox brain extract powder, 5-15 parts of ox heart extract powder, 5-15 parts of peptones, 2.5-7.5 parts of sodium chloride, 1-3 parts of glucose, 1.5-3.5 parts of disodium hydrogen phosphate, 1-3 parts of mannitol, 1-3 parts of sodium pyruvate, 1-3 parts of glycine, 1-3 parts of aesculin, and 1000 parts of distilled water, and a pH value of the composite enrichment medium is 7.2-7.5. A preparation method for the composite enrichment medium comprises the following steps: adding the components into distilled water according to the formula, stirring and dissolving the components, regulating the pH value of the mixture, and sterilizing the mixture at high pressure. The five bacteria are main pathogenic bacteria which are needed to be detected according to the national food hygienic standard. The composite enrichment medium can be used for realizing rapid proliferation of the five pathogenic bacteria. After carrying out bacteria enrichment for 16 hours, the concentration of the thallus can be increased to 10<6>CFU/mL-10<9>CFU/mL from 1CFU/mL, so that the thallus can be directly used for carrying out high flux detection on multiple PCRs (polymerase chain reactions), gene chips and micro-fluidic chips, and thus, screening of the five pathogenic bacteria is completed.

The invention relates to the field of stem cells and discloses a culture method of deciduous tooth pulp stem cells. The culture method includes: taking pulp tissue, cutting the pulp tissue into pieces, and then adding I-type collagenase; digesting for 10-20min under conditions of 37 DEG C and 200rpm; stopping digestion of a serum-free culture medium; blowing and beating discrete cell mass to obtain single discrete cells; adding cell suspension to re-suspend the cells, and adjusting cell density; culturing the cells in a carbon dioxide incubator at temperature of 37 DEG C and with humidity of 95%; when the cells grow to be fused by 80-90%, using digestive liquid of trypsase to digest the cells before passage culture, wherein the cell suspension is composed of the serum-free culture medium, epidermal growth factor and basic fibroblast growth factor. The cells cultured by the method are good in shape, have tendency of fusiform cluster growing and are high in activity and quick in proliferation, good stem cell characteristics of the deciduous tooth pulp stem cells can be maintained, and the culture method is suitable for large-scale culture of the deciduous tooth pulp stem cells.

The invention relates to a method for treating kitchen wastes by utilizing photosynthetic bacteria and recycling the kitchen wastes. The method particularly comprises a kitchen waste pretreating method which includes carrying out hydrolysis acidification treatment on the kitchen wastes until the concentration of volatile fatty acid is up to be 5000-15000mg/L to obtain hydrolysis acidification liquid, performing solid and liquid separation on the hydrolysis acidification liquid, adjusting the pH value of the liquid to be 6.5-7.5 to obtain a pretreated material, and a method for culturing the photosynthetic bacteria by using the pretreated material, which comprises the following steps of: inoculating 100-1000mg/L of a photosynthetic bacteria flora in the pretreated material and culturing the photosynthetic bacteria flora for 3-5 days under conditions that the illuminance is 1500-5000lux, the temperature is 25-35 DEG C and the dissolved oxygen concentration is 0-0.8mg/L. A product obtained by using the method can be used for preparing feed additives, water purification agents, ecological organic fertilizers or aquaculture baits. The method is strong in poison resistance capability for treating the kitchen wastes, good in denitrification and high in organic matter removal capability.

The invention relates to a titanium oxide composite coating and a preparation method thereof. The titanium oxide composite coating comprises a porous titanium oxide coating formed in situ on the surface of a titanium-based metal base material by virtue of a micro-arc oxidation technique, and ferric oxide nanoparticles mixed with the porous titanium oxide coating by virtue of a plasma-immersion ion implantation technique, wherein the content of the iron element in the titanium oxide composite coating ranges from 1% to 15%. The invention also provides a method for preparing the titanium oxide composite coating, and the method comprises the steps of (1) forming the porous titanium oxide coating in situ on the surface of the titanium-based metal base material by virtue of the micro-arc oxidation technique, and (2) implanting iron ions into the titanium oxide coating by virtue of the plasma-immersion ion implantation technique to form the ferric oxide nanoparticle modified titanium oxide composite coating.