Method for cultivating detoxification tissue culture bulb of fritillaria thunbergii

Abstract

The present invention relates to a culture method for the detoxication and the tissue culture of the bulb of Fritillaria thunbergii, which belongs to the technical field of breeding the plant seedling and is specially used for breeding the detoxic seedling of Fritillaria thunbergii and the detection of the virus of Fritillaria thunbergii. The method comprises the steps such as the formulation of the culture medium, the culture of the detoxication tissue culture bulb, the detection of the virus ELISA, etc. The present invention has good detoxication effect, high sensitivity of the virus detection, and can guarantee that the detocication daughter bulb is free from virus, thereby effectively controlling the occurrence and the broadcasting of the virus disease; the multiplication of the bulb is fast, and the monthly multiplication rate can reach 4.5 times, thereby being applicable to the factory seedling raising, greatly saving the breeding land of the traditional method and obviously reducing the production cost of Fritillaria thunbergii; the present invention can be applied to the purification and rejuvenation and the development of the species of Fritillaria thunbergii.

Description

technical field

[0001] The invention relates to the technical field of plant detoxification tissue culture rapid propagation, in particular to a method for cultivating the detoxification tissue culture bulbs of Fritillaria fritillaria. Background technique

[0002] Fritillaria thunbergii Miq. (Fritillaria thunbergii Miq.) is a plant of the genus Fritillaria in the family Liliaceae. Its dried bulbs are one of the famous Chinese medicinal materials "Zhe Bawei". Zhejiang Fritillaria is native to Xiangshan County, so it is also called Elephant Fritillaria. It is said that during the Kangxi period of the Qing Dynasty, farmers in Xiangshan began to transfer Fritillaria from wild to home. For a long time, Pan'an and Yinzhou are the main producing areas of Fritillaria fritillaria, accounting for about 70% of the total output of Fritillaria fritillata in China. It is also cultivated in Jiangsu, Jiangxi, Shanghai, Hubei and Hunan provinces. With the in-depth adjustment of planting i...