Method for cultivating detoxification tissue culture bulb of fritillaria thunbergii

A cultivation method, the technology of Fritillaria fritillaria, is applied in the field of cultivation of Fritillaria fritillata virus-free tissue culture bulbs, which can solve the problems of high production cost, poor quality, and low yield, and achieve saving land for breeding, improving product quality, reducing The effect of production costs

Inactive Publication Date: 2008-07-09
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to adopt large bulbs as seeds in the existing Fritillaria fritillaria conventional production and seed propagation technology, the reproduction coefficient is extremely low, the amount of seeds used is extremely large, the underground dormancy period of seeds is long, the area of ​​production and seed retention is large, and there are Plant virus infection leads to defects such as poor quality, low yield, and high production cost; provide a kind of bulb that can reproduce in large quantities and quickly, and can remove the virus carried by the bulb and have been specifically tested to ensure that the bulb of Fritillaria fritillaria is non-toxic. Complementary methods of virus, tissue culture and detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A kind of culture method of Fritillaria fritillaria detoxification tissue culture bulb, carry out according to the following steps:

[0052] (1) The preparation of the culture medium, including each component of the basic culture medium and the culture medium of each stage of tissue culture and the weight per liter:

[0053] 1) Basic medium: MS medium for subbulb induction, proliferation, strong seedlings and bulb growth medium; 1 / 2 MS medium for bulb rooting medium; wherein, agar 7g / L, pH5.8;

[0054] 2) Daughter bulb induction medium: sucrose 30g / L MS+2,4-D 1mg / L+ZT 2mg / L;

[0055] 3) Daughter bulb proliferation medium: MS+BA 2mg / L+NAA 2mg / L+thiamine hydrochloride (VB 1 )4mg / L;

[0056] 4) Strong seedling medium: MS+BA 1mg / L+NAA 1mg / L+thiamine hydrochloride (VB 1 )4mg / L;

[0057] 5) Bulb growth medium: white sugar 40g / L MS+BA 0.5mg / L and NAA 0.5mg / L+thiamine hydrochloride (VB 1 )4mg / L+hydrolyzed casein (CH)500mg / L;

[0058] 6) Rooting medium: 1 / 2MS of white suga...

Embodiment 2

[0080] In this example, the agar of the basic medium is 9g / L, and the pH is 5.6; the subbulb induction medium is: MS+2,4-D0.5mg / L+ZT 1mg / L of sucrose 20g / L; Proliferation medium is: white sugar 30g / L MS+BA 1mg / L+NAA3mg / L+thiamine hydrochloride (VB 1 )4mg / L; strong seedling medium is: white sugar 20g / L MS+BA1.5mg / L and NAA0.5mg / L+thiamine hydrochloride (VB 1 ) 4mg / L; bulb growth medium is: white sugar 20g / L MS+BA 0.1mg / L+NAA 1mg / L+thiamine hydrochloride (VB 1 ) 4mg / L+ hydrolyzed casein (CH) 500mg / L; rooting medium: white sugar 30g / L 1 / 2MS+NAA 0.1mg / L+ thiamine hydrochloride (VB 1 ) 4mg / L; take the young stems of Fritillaria fritillata, soak them in 75% alcohol for 0.5min, then soak them in 0.1% mercuric chloride aqueous solution for 10min, and finally rinse them with sterile water for 3 to 5 times for sterilization. Take explant materials for virus-free tissue culture; treat strong seedlings at 35°C, light intensity 2000-3000Lx, light time 12h / d, and heat them for 2 weeks, th...

Embodiment 3

[0082] In this example, the agar of the basic medium is 8g / L, and the pH is 5.7; the subbulb induction medium is: sucrose 40g / L MS+2,4-D1.5mg / L+ZT 3mg / L; Proliferation medium is: white sugar 20g / L MS+BA 3mg / L+NAA1mg / L+thiamine hydrochloride (VB 1 )4mg / L; strong seedling medium is: white sugar 30g / L MS+BA0.5mg / L and NAA1.5mg / L+thiamine hydrochloride (VB 1 ) 4mg / L; bulb growth medium is: white sugar 30g / L MS+BA 1mg / L+NAA 0.1mg / L+thiamine hydrochloride (VB 1 ) 4mg / L+ hydrolyzed casein (CH) 500mg / L; rooting medium: white sugar 40g / L 1 / 2MS+NAA 1mg / L+ thiamine hydrochloride (VB 1 ) 4mg / L; take the newly grown pedicel of Fritillaria japonica, soak it in 75% alcohol for 1.0min, then soak it in 0.1% mercuric chloride aqueous solution for 15min, and finally rinse it with sterile water for 3 to 5 times for sterilization. explant materials for virus-free tissue culture; heat-treat strong seedlings at 35°C, light intensity 2000-3000Lx, and light time 12h / d for 4 weeks, then use for shoot...

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Abstract

The present invention relates to a culture method for the detoxication and the tissue culture of the bulb of Fritillaria thunbergii, which belongs to the technical field of breeding the plant seedling and is specially used for breeding the detoxic seedling of Fritillaria thunbergii and the detection of the virus of Fritillaria thunbergii. The method comprises the steps such as the formulation of the culture medium, the culture of the detoxication tissue culture bulb, the detection of the virus ELISA, etc. The present invention has good detoxication effect, high sensitivity of the virus detection, and can guarantee that the detocication daughter bulb is free from virus, thereby effectively controlling the occurrence and the broadcasting of the virus disease; the multiplication of the bulb is fast, and the monthly multiplication rate can reach 4.5 times, thereby being applicable to the factory seedling raising, greatly saving the breeding land of the traditional method and obviously reducing the production cost of Fritillaria thunbergii; the present invention can be applied to the purification and rejuvenation and the development of the species of Fritillaria thunbergii.

Description

technical field [0001] The invention relates to the technical field of plant detoxification tissue culture rapid propagation, in particular to a method for cultivating the detoxification tissue culture bulbs of Fritillaria fritillaria. Background technique [0002] Fritillaria thunbergii Miq. (Fritillaria thunbergii Miq.) is a plant of the genus Fritillaria in the family Liliaceae. Its dried bulbs are one of the famous Chinese medicinal materials "Zhe Bawei". Zhejiang Fritillaria is native to Xiangshan County, so it is also called Elephant Fritillaria. It is said that during the Kangxi period of the Qing Dynasty, farmers in Xiangshan began to transfer Fritillaria from wild to home. For a long time, Pan'an and Yinzhou are the main producing areas of Fritillaria fritillaria, accounting for about 70% of the total output of Fritillaria fritillata in China. It is also cultivated in Jiangsu, Jiangxi, Shanghai, Hubei and Hunan provinces. With the in-depth adjustment of planting i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00C12N5/04
Inventor 陈剑平徐刚郑红英林林汪一婷程晔吕永平牟豪杰
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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