27 results about "Thiamine Hydrochloride" patented technology

The invention relates to the technical field of bacterium enrichment nutrient solutions, in particular to a bacterium enrichment nutrient solution for fermentation and a preparation method of the bacterium enrichment nutrient solution. The culture medium is prepared from the following raw materials in parts by weight: maltodextrin, crystalline glucose, low-pigment yeast extract powder, refined soy peptone, dipotassium phosphate, monopotassium phosphate, magnesium sulfate heptahydrate, sodium chloride, phytic acid, L-ascorbic acid, manganese sulfate monohydrate, biotin, thiamine hydrochloride and choline chloride. Through a double-carbon-source structure, maltodextrin and crystalline glucose cooperate, metabolic oscillation is avoided, and the energy utilization rate is increased; the low-pigment yeast extract powder and the refined soy peptone are complementary, so that the rapid growth requirement of thalli is met; phytic acid is chelated with metal ions to prevent precipitation, L-ascorbic acid is used for resisting oxidation, and biotin and thiamine activate a coenzyme pathway to realize accurate regulation and control of a metabolic network.

The invention provides a formula of a sports nutritious food and a preparation method thereof, and the formula comprises the following components: edible glucose, sorbitol, bitter gourd powder, Chinese yam powder, poria cocos powder, lucid ganoderma (ganoderma sinensis), ground cinnamon, oyster powder, licorice powder, thiamine hydrochloride and riboflavin. The food formula contains multiple natural food materials such as lucid ganoderma (ganoderma sinensis), bitter gourds, Chinese yams, poria cocos, cinnamon, oysters and liquorice, and nutritional ingredients such as edible glucose, sorbitol, thiamine hydrochloride and riboflavin, so that rich nutritional support can be provided for sports people, the body requirements of the sports people can be met, and the health of the sports people can be improved. According to the present invention, the edible glucose and the sorbitol can provide the rapid energy source for the body, the physical ability and the endurance of the sporter can be easily improved so as to improve the athletic performance, and the product is convenient to carry and eat after the packaging, and is suitable for the sporter to supplement the nutrition before and after the exercise or in the daily life at any time.

This invention discloses a method for efficient genetic transformation of rice that overcomes genotype dependence and a dedicated culture medium, relating to the field of biotechnology. The dedicated culture medium comprises KCl, KH₂PO₄, MgSO₄·7H₂O, CaCl₂·2H₂O, disodium ethylenediaminetetraacetate, FeSO₄·7H₂O, MnSO₄·H₂O, ZnSO₄·7H₂O, H₃BO₃, KI, CoCl₂·6H₂O, CuSO₄·5H₂O, Na₂MoO₄·2H₂O, glutamine, aspartic acid, arginine, glycine, cyclohexanehexyl alcohol, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid, and a carbon source. Using this dedicated culture medium can increase the number of rice cells containing the NiR gene. 375 S 453 The genetic transformation efficiency of haplotype rice varieties is of great significance for overcoming the bottleneck of rice genotype dependence, promoting functional genomics research and molecular breeding.

This invention provides an induction culture medium and method for using marigold anthers as explants. The induction culture medium is based on MS solid medium containing agar, with specific amounts of sucrose, tryptophan, phenylalanine, arginine, casein amino acids, anhydrous asparagine, 6-benzylpurine, naphthaleneacetic acid, silver nitrate, pyridoxine hydrochloride, thiamine hydrochloride, nicotinic acid, D-biotin, folic acid, ascorbic acid, and coconut juice added. This invention also provides a method for induction culture using this induction culture medium. This invention can greatly improve the callus induction rate of marigolds, significantly increasing the number of regenerated seedlings, and provides technical guidance for the cultivation of haploid marigolds using anthers.

The invention discloses an efficient rice genetic transformation method capable of overcoming genotype dependence and a special culture medium, and relates to the technical field of biology. The special culture medium is prepared from KCl, KH2PO4, MgSO4. 7H2O, CaCl2. 2H2O, disodium ethylene diamine tetraacetate, FeSO4. 7H2O, MnSO4. H2O, ZnSO4. 7H2O, H3BO3, KI, CoCl2. 6H2O, CuSO4. 5H2O, Na2MoO4. 2H2O, glutamine, aspartic acid, arginine, glycine, inositol, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid and a carbon source. By adopting the special culture medium, the genetic transformation efficiency of a rice variety containing the NiR gene E375S453 haplotype can be improved, and the special culture medium has important significance in overcoming the bottleneck of rice genotype dependence and promoting functional genome research and molecular breeding.

The application provides a sulfate-reducing bacteria culture medium and application thereof, and components of the culture medium include: a carbon source, a nitrogen source, a phosphorus source, calcium chloride, magnesium chloride, an inorganic sulfur source, an organic sulfur compound with reducibility, inorganic trace elements, agar and water; wherein the mass content of the organic sulfur compound with reducibility is 5-30 mg / L, the mass content of the inorganic trace elements is 152-365 mg / L; the organic sulfur compound with reducibility includes lipoic acid and / or thiamine hydrochloride; and the inorganic trace elements include a combination of zinc elements, manganese elements, molybdenum elements and boron elements. The application introduces inorganic trace elements required by biological cell metabolism and rich sulfur electron acceptor species, promotes the growth and metabolism process of sulfate-reducing bacteria, and enables SRB cells to form visible colonies at a faster speed.

The invention relates to a high-yield vitamin B12 strain with improved robustness and application of the high-yield vitamin B12 strain, and belongs to the technical field of microbial fermentation and metabolic engineering. According to the invention, a pseudomonas denitrificans original production strain capable of synthesizing the vitamin B12 is evolved by a long-term adaptive laboratory taking the vitamin B12 and a structural analogue thiamine hydrochloride thereof as selection pressure. The evolved strain shows significantly enhanced growth ability and fermentation stability in a high stress environment, and the highest yield of vitamin B12 can reach 314 mg / L, which is 38.7% higher than that of an original strain, which is the highest level reported at present. Moreover, stable production can be realized on a 7 L fermentation tank, and good batch stability is realized. The invention also provides application of the strain in industrial production of the vitamin B12, and the strain has important significance in reducing the production cost of the vitamin B12 and improving the fermentation efficiency.

This invention belongs to the field of biomedical technology, and specifically relates to a nutrient composition for the prevention and / or treatment of post-traumatic stress disorder (PTSD). This invention utilizes metabolomics technology to screen and identify metabolite changes occurring during the pathophysiological process of PTSD, and then designs a nutrient composition comprising five components: α-ketoglutarate, thiamine hydrochloride, L-serine, L-tryptophan, and L-tyrosine. Combined supplementation of these five nutrient components effectively improves anxiety-like behavior and promotes the extinction of fear memories in PTSD model mice. Furthermore, the nutrient composition of this invention exhibits extremely high safety, with side effects far lower than traditional chemically synthesized drugs, and is expected to improve long-term patient adherence.

The invention provides a method for recovering thiamine hydrochloride from thiamine hydrochloride mother liquor and application of the method, and relates to the technical field of mother liquor recovery, the method comprises the following steps: evaporating methanol from the thiamine hydrochloride mother liquor, then freezing, and recovering to obtain thiamine hydrochloride; wherein the evaporation capacity of the methanol is 45%-65%. The technical problem that in the prior art, high-COD and high-ammonia-nitrogen wastewater is generated when the thiamine hydrochloride mother liquor is treated is solved, and the technical effects that effective materials in the thiamine hydrochloride mother liquor are comprehensively utilized, thiamine hydrochloride in the mother liquor is recycled to the maximum extent, waste is turned into wealth, and the production cost is reduced are achieved.

The invention relates to the technical field of food and beverage development, in particular to a cherry sleep-aiding beverage and a preparation method thereof. The natural components of the lucid ganoderma, the spina date seeds and the lavandula angustifolia, the aminobutyric acid and the theanine are added, so that a basis is mainly provided for the sleep-aiding function of the cherry sleep-aiding drink; meanwhile, medium-chain triglyceride, thiamine hydrochloride, propylene glycol alginate, lysine and magnesium carbonate are introduced, so that the lucid ganoderma, the spina date seeds, the lavandula angustifolia, the aminobutyric acid and the theanine stably exist in the cherry sleep-aiding beverage, and the possible chemical reaction process with other components in the storage process is avoided; the function weakening and even disappearance caused by interference of other components can be avoided, and the sleep aiding function can be enhanced; the cherry concentrated solution, the sodium citrate, the vitamin C and the pectin are introduced, so that a cherry flavor and a stable beverage system are obtained, and the uniformity and the stability of each component are ensured.

The application relates to the technical field of microbial culture medium, and discloses a chemical component limited culture medium for supporting growth of bifidobacterium and application thereof. The CDM comprises a carbon source, salts, amino acids, vitamins and water; the carbon source is selected from at least one of glucose, fructose, lactose, maltose and galactooligosaccharide, the salts comprise sodium acetate, potassium hydrogen phosphate, ammonium acetate, magnesium sulfate heptahydrate, ferrous sulfate heptahydrate, sodium chloride and manganese sulfate monohydrate, the amino acids comprise alanine, cystine, cysteine, tryptophan and asparagine, and the vitamins comprise pyridoxine hydrochloride, nicotinic acid, calcium pantothenate, thiamine hydrochloride, riboflavin, p-aminobenzoic acid, folic acid and biotin. The CDM of the application is a liquid culture medium, only comprises 21 definite components, is simple in components, convenient in preparation, and is high in universality, and can effectively support growth of bifidobacterium.

The invention belongs to the field of microbial culture media. The invention provides a chemical component limited culture medium for escherichia coli fermentation culture and plasmid expression, which comprises the following components: glucose, thiamine hydrochloride, diammonium hydrogen phosphate, anhydrous magnesium sulfate, sodium chloride, manganese sulfate monohydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, sodium molybdate dihydrate, boric acid, L-glutamic acid, calcium chloride dihydrate and citric acid. The culture medium is clear in chemical component, free of serum and protein and free of plant hydrolysate, nutrient supply of escherichia coli in the whole fermentation process is guaranteed through the components of the addition amount, normal metabolism and growth of escherichia coli are promoted through synergistic interaction, and the yield of escherichia coli is increased. The amount OD600 of thalli cultured for 24 hours can reach and exceed the level of an imported culture medium of a contrast; the yield of the expressed plasmid can reach 52.58 [mu] g / mL, which is obviously higher than the level of 29.38 [mu] g / mL of a control culture medium, and the method has obvious advantages.

The invention relates to the field of compound preparation, in particular to a method for preparing thiamine hydrochloride. The method comprises the following steps: carrying out contact reaction on thiamine nitrate, ammonium chloride and hydrogen chloride under the condition of an organic solvent, and separating reaction liquid to obtain thiamine hydrochloride solid. Compared with the prior art, ammonium chloride is used for replacing part of hydrochloric acid to participate in the reaction in the thiamine hydrochloride production process, so that the dosage of hydrochloric acid is reduced, equipment corrosion can be reduced, subsequent wastewater treatment steps are simplified, the product yield is not influenced, the content of other impurities is not increased, new production equipment is not added, and the production cost is effectively saved; furthermore, the materials are recycled, so that a more environment-friendly and cost-saving process route can be realized.

The invention discloses a culture medium for promoting germination of saussurea involucrata seeds and increasing the flavone content in a cell culture and application of the culture medium. Multivitamins for promoting germination of saussurea involucrata seeds and increasing the flavone content in the cell culture comprise thiamine hydrochloride VB1, nicotinic acid and pyridoxine hydrochloride VB6; wherein the concentration of thiamine hydrochloride VB1 is 1000 mg / L, the concentration of nicotinic acid is 500 mg / L, and the concentration of pyridoxine hydrochloride VB6 is 500 mg / L. A mixed mother solution (V3 vitamin for short) of three vitamins is prepared for filtration, sterilization and addition, the concentrations of the three vitamins are thiamine hydrochloride VB11000 mg / L, nicotinic acid 500 mg / L and pyridoxine hydrochloride VB6500 mg / L respectively, the overall addition amount of V3 is 1 mL / L, the germination rate of saussurea involucrata seeds can be effectively improved, and the germination rate of the seeds is 40.58%.

The application provides fluorescent carbon dots with room-temperature phosphorescence performance and a preparation method and application thereof, and belongs to the technical field of carbon dots. The preparation method provided by the application comprises the following steps: (1) mixing polyethyleneimine, thiamine hydrochloride and water to obtain a mixed solution; (2) performing hydrothermal reaction on the mixed solution obtained in the step (1) to obtain fluorescent carbon dots with room-temperature phosphorescence performance. The carbon dots prepared by using polyethyleneimine and thiamine hydrochloride as raw materials have both fluorescent and phosphorescent characteristics, and have high fluorescent intensity and long phosphorescent afterglow time.

The present invention belongs to the field of tissue culture technology and specifically relates to a culture medium for growing ginger seedlings and a method for growing ginger. Each 1000 mL of the culture medium contains: 20-25 g sucrose, 10-13 g agar, 0.16-0.19 g Hericium erinaceus fermentation product, 0.10-0.14 g plant fermentation product, 120-150 mg inositol, 580-600 mg KNO3, 130-150 mg NH4H2PO4, 240-270 mg MgSO4·7H2O, 20-24 mg 6-BA, 3-6 mg thiamine hydrochloride, 150-180 mg CaCl2, etc. The culture medium of the present invention can improve the survival rate of ginger seedlings, significantly increase the proliferation coefficient, and have a high rooting rate.

The present application relates to a kind of method for improving the content of meaty substance in thiamine degradation product and the application of degradation product, belong to food processing technical field.Through the thiamine hydrochloride, food additive and solvent are added in closed reaction container, stirring reaction 10-180min under 80-140 ℃, after reaction, cooling, obtain thiamine degradation product.The method can greatly improve the generation amount of meaty compound in thiamine degradation process, thiamine degradation product can be directly used in reaction essence preparation, enhance meaty, reduce cost.In addition, by edible oil or capric caprylic acid glyceride, thiamine degradation product is extracted, separated and dried, obtain meaty seasoning oil, remove bitter substance in taste, for meaty reaction or flavoring product, also can play the role of enhancing meaty, reducing cost, with wide application prospect.

The invention provides a fluorescent carbon dot with room-temperature phosphorescence performance as well as a preparation method and application thereof, and belongs to the technical field of carbon dots. The preparation method provided by the invention comprises the following steps: (1) mixing polyethyleneimine, thiamine hydrochloride and water to obtain a mixed solution; and (2) carrying out hydrothermal reaction on the mixed solution obtained in the step (1) to obtain the fluorescent carbon dots with room-temperature phosphorescence performance. Polyethyleneimine and thiamine hydrochloride are adopted as raw materials, and the prepared carbon dots have fluorescence and phosphorescence characteristics and are high in fluorescence intensity and long in phosphorescence afterglow time.

The application discloses a sugar-free endurance sports food and a preparation method thereof. Product raw materials include retinol acetate oil, cholecalciferol oil, dl-alpha-tocopherol acetate oil, thiamine hydrochloride, riboflavin, pyridoxal hydrochloride, L-ascorbic acid, caffeine, taurine, magnesium sulfate, zinc gluconate, selenium-rich yeast, chromium-rich yeast, emulsifier, sweetener, acidity regulator and dietary fiber. The preparation process is prepared by spray drying and mixing of raw materials, and the raw materials include trace nutrients, emulsifiers, sweeteners, acidity regulators and dietary fibers. Modern granulation, embedding and slow-release preparation technologies are utilized to continuously provide nutrition during sports and realize true sugar-free. The raw materials only contain a small amount of Na + , and will not affect the osmotic pressure of the human body, and are more suitable for supplementing endurance sports.

Provided is a transfection reagent, comprising a solvent, organic substances, amino acids, vitamins and inorganic salts, wherein the organic substances comprise D-glucose, sodium pyruvate, i-inositol, phenol red and choline chloride; the amino acids comprise glycine, L-glutamine, L-valine, L-isoleucine, L-leucine, L-threonine, L-lysine hydrochloride, L-phenylalanine, L-serine, L-tyrosine disodium salt, L-arginine hydrochloride, L-methionine, L-cystine dihydrochloride, L-histidine hydrochloride and L-tryptophan; the vitamins comprise nicotinamide, pyridoxine hydrochloride, thiamine hydrochloride, folic acid, D-calcium pantothenate and riboflavin; and the inorganic salts comprise sodium chloride, sodium bicarbonate, potassium chloride, calcium chloride, sodium dihydrogen phosphate, magnesium sulfate, hydrochloric acid and ferric nitrate. The transfection reagent is suitable for a wide range of applications, achieves the rapid onset of transfection, and has a good transfection effect.

The invention relates to the technical field of food processing and condiments, in particular to a flavor enhancer based on spice synergy and biotechnology, a preparation method of the flavor enhancer and application of the flavor enhancer to seasoning in dish processing. Wherein the aroma enhancing paste comprises the following raw materials: concentrated duck soup paste, concentrated duck bone enzymatic hydrolysate, monosaccharide, compound amino acid, hydrolyzed vegetable protein and thiamine hydrochloride, and the concentrated duck soup paste is obtained by sequentially crushing duck bones, cleaning, boiling, performing enzymolysis with lipase, adding spices, performing enzymolysis with cellulase, filtering, inactivating and concentrating. The concentrated duck bone enzymatic hydrolysate is obtained by adding water into bone residues obtained by filtering in a preparation method of concentrated duck soup paste and then sequentially carrying out protease enzymolysis, inactivation, filtering and concentration, and the flavor enhancer is used for processing dish foods, so that the problems of insufficient flavor and the like in industrial dishes can be effectively solved.

The present invention relates to a transfection reagent and its application, belonging to the field of biotechnology. The present invention provides a transfection reagent, the components of which include solvent, organic matter, amino acid, vitamin and inorganic salt; organic matter includes D-glucose, sodium pyruvate, i-inositol, phenol red and choline chloride; amino acid includes glycine, L-glutamine, L-valine, L-isoleucine, L-leucine, L-threonine, L-lysine hydrochloride, L-phenylalanine, L-serine, L-tyrosine disodium salt, L-arginine hydrochloride, L-methionine, L-cystine dihydrochloride, L-histidine hydrochloride and L-tryptophan; vitamins include nicotinamide, pyridoxine hydrochloride, thiamine hydrochloride, folic acid, D-calcium pantothenate and riboflavin; inorganic salt includes sodium chloride, sodium bicarbonate, potassium chloride, calcium chloride, sodium dihydrogen phosphate, magnesium sulfate, hydrochloric acid and ferric nitrate. This transfection reagent has a wide range of applications, fast transfection onset time and good transfection effect.

The invention relates to the technical field of culture media, in particular to a streptococcus suis enrichment culture medium for high-density fermentation and a preparation method of the streptococcus suis enrichment culture medium. Tryptone; a soluble starch; glucose; sodium azide; a polymyxin B sulfate; l-cysteine hydrochloride is added; a buffer salt; an ion supplement; a cell wall relaxant; thiamine hydrochloride; an antioxidant; a composite nutrient source; an osmotic pressure regulator; growth factors. According to the culture medium, quick-acting glucose and slow-release starch are used for synergistically supplying energy, quick start and continuous metabolism are both considered, and the enrichment period is greatly shortened; polymyxin B is introduced to be combined with a sodium azide targeted inhibitor, so that gram-negative bacteria and part of positive infectious microbes are accurately inhibited, and the selectivity is remarkably improved; meanwhile, the activity of thalli is maintained by an anti-oxidation and buffer system, high-sensitivity detection of low-concentration samples is ensured, and the application scenario of efficient detection and industrial fermentation of streptococcus suis is conformed.

The invention relates to the technical field of food nutrition, and particularly discloses a composition for strengthening physique and improving resistance, which is prepared from the following raw materials by weight: 40 to 100 g of L-ascorbic acid, 0.5 to 2 g of cyanocobalamin, 0.2 to 1.2 g of thiamine hydrochloride, 0.2 to 1.2 g of riboflavin, 0.1 to 0.8 g of pyridoxine hydrochloride, 10 to 30 g of zinc lactate, 0.5 to 2 g of 2% selenized carrageenan, 15500 to 21500 g of milk powder, 600 to 1400 g of lactose and 5 to 15 g of stevioside. Especially, the plant extract, the amino acid and the antioxidant are further added for combination, so that the immunity of the composition can be remarkably improved; the formula is designed according to the principles of modern nutriology and traditional herbal medicine, can provide various vitamins, minerals, amino acids and herbal extracts required by the human body in daily life, and is helpful for enhancing the defense mechanism of the body and improving the reaction of the immune system.

The invention relates to the technical field of brightener processing, in particular to an acid copper plating brightener, an acid copper plating electroplating solution and an electroplating technology of the acid copper plating brightener. The cleaning agent is prepared from the following raw materials by concentration: 50-100 g / L of octadecylamine polyoxyethylene ether, 10-20 g / L of thiamine hydrochloride, 5-30 g / L of polydiaminourea, 20-40 g / L of aspirin sodium and 80-120 g / L of a surfactant. According to the brightening agent, through the synergistic effect of all the components, the problems that a traditional brightening agent is prone to scorching, fogging and coarse crystallization under the high current density are effectively solved, the contradiction between deep hole filling and plate surface brightness is balanced, the dog-bone-shaped distribution and cavity defects in high-thickness-diameter-ratio through hole electroplating are overcome, and overplating and stripes in a low-current area are avoided. The obtained copper plating layer is fine in crystallization, bright and flat, the interlayer interconnection reliability and the microscopic uniform plating capacity are remarkably improved, the method is matched with the VCP vertical continuous electroplating technology, and the light, thin and high-frequency manufacturing requirements of high-density and fine circuit PCBs are met.

This invention provides a low-salt flavored pork bone paste and its preparation method, belonging to the field of food processing technology. The preparation method includes the following steps: using pork bone paste as raw material, a basic enzymatic hydrolysate is prepared by low-temperature ultrasound-assisted papain hydrolysis; thiamine hydrochloride, D-xylose, and L-cysteine ​​hydrochloride are sequentially added to the basic enzymatic hydrolysate to conduct a Maillard reaction, resulting in a hot reaction solution; a flavor regulator and a thickener are added to the hot reaction solution to obtain the low-salt flavored pork bone paste. This invention efficiently prepares an enzymatic hydrolysate substrate rich in small molecule peptides and amino acids through low-temperature intermittent ultrasound-assisted enzymatic hydrolysis; it employs a three-step Maillard reaction with stepwise feeding to directionally generate core meat flavor substances and avoid sulfurous odors; furthermore, a low-salt formula with potassium chloride as the main salt and synergistic stability of compound phosphate and calcium lactate can be used to significantly reduce sodium content and give the product a delicate and stable paste texture.

This invention discloses a bergamot effervescent tablet based on microencapsulation technology and its preparation method, relating to the field of solid beverage technology. The invention comprises, based on a total feed weight of 100 kg, the following components: bergamot extract; 3 kg; anhydrous citric acid; 18 kg; sodium bicarbonate; 15 kg; dextrin; 45 kg; gum arabic; 0.6 kg; tragacanth gum; 0.15 kg; taurine; 1.58 kg; zinc gluconate; 0.75 kg; zinc citrate; 0.25 kg; calcium gluconate; 0.25 kg; ascorbic acid C; 0.1 kg; ferrous gluconate; 0.11 kg; thiamine hydrochloride; 0.058 kg; riboflavin; 0.03 kg; pyridoxine hydrochloride; 0.02 kg; cyanocobalamin; 0.001 kg; folic acid; 0.001 kg; isomaltitol; 15 kg; sucralose; 0.1 kg. The advantages are: through systematic screening of key factors such as the inclusion of aromatic oil components in bergamot extract, the ratio of acid-base disintegrants, the ratio of flavoring agents and food fortifiers, the type of fillers, the preparation method of acid-base particles, and the tableting process parameters.