Culture medium for delaying growth of potato tissue cultured seedlings

A technology of growth rate and medium, which is applied in the field of medium for delaying the growth rate of potato tissue culture seedlings, can solve the problems of unfavorable long-term storage, excessive labor and materials, and aging of core seedlings, and achieve the reduction of labor and materials, The effect of strong plant growth and thick stems

Inactive Publication Date: 2014-06-25
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, one of the ways to preserve potato core seedlings at home and abroad is to use tissue culture, and MS medium is generally used for preservation, but the tissue cultured seedlings grown on MS medium need to be cut after about 30 days Transplanting once requires a lot of labor and materials, and such frequent operations will accelerate the physiological age of the core seedlings, which is not conducive to long-term storage

Method used

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Embodiment 1

[0023] A kind of culture medium that delays the growth rate of potato tissue culture seedlings, in 1L medium, consists of the following components: ammonium nitrate (NH 4 NO 3 ) 660mg, potassium nitrate (KNO 3 ) 760mg, calcium chloride (CaCl 2 2H 2 O) 176mg, magnesium sulfate (MgSO 4 ·7H 2 O) 168mg, potassium dihydrogen phosphate (KH 2 PO 4 ) 68mg, potassium iodide (KI) 0.332mg, boric acid (H 3 BO 3 ) 2.48mg, manganese sulfate (MnSO 4 4H 2 O) 6.76mg, zinc sulfate (ZnSO 4 ·7H 2 O) 3.44mg, sodium molybdate (Na 2 MoO 4 2H 2 O) 0.1mg, copper sulfate (CuSO 4 ·5H 2 O) 0.01mg, cobalt chloride (CoCl 2 ·6H 2 O) 0.01mg, sodium ferric edetate (FeNa 2 EDTA·H 2 O) 30mg, inositol (C 6 h 12 o 6 ) 40mg, niacin (C 6 h 5 NO 2 ) 0.2mg, pyridoxine hydrochloride (C 8 h 11 NO 3 HCl) 0.2mg, thiamine hydrochloride (C 12 h 17 ClN 4 OS·HCl) 0.04mg, sucrose (C 12 h 22 o 11 ) 8000mg, mannitol 8000mg, agar 7000mg, and the balance is water.

[0024] The preparation metho...

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Abstract

The invention discloses a culture medium for delaying the growth of potato tissue cultured seedlings. Every 1L of the culture medium comprises the following components: 627mg-693mg of ammonium nitrate (NH4NO3), 722mg-798mg of potassium nitrate (KNO3), 168mg-184mg of calcium chloride (CaCl2.2H2O), 160mg-176mg of magnesium sulfate (MgSO4.7H2O), 65mg-71mg of monopotassium phosphate (KH2PO4), 0.315mg-0.348mg of potassium iodide (KI), 2.36mg-2.60mg of boric acid (H3BO3), 6.43mg-7.09mg of manganese sulfate (MnSO4.4H2O), 3.27mg-3.61mg of zinc sulfate (ZnSO4.7H2O), 0.095mg-0.105mg of sodium molybdate (Na2MoO4.2H2O), 0.009mg-0.011mg of copper sulfate (CuSO4.5H2O), 0.009mg-0.011mg of cobalt chloride (CoCl2.6H2O), 28.5mg-31.5mg of ferric sodium edelate (FeNa2EDTA.H2O), 38mg-42mg of inositol (C6H12O6), 0.19mg-0.21mg of nicotinic acid (C6H5NO2), 0.19mg-0.21mg of pyridoxine hydrochloride (C8H11NO3.HCl), 0.038mg-0.042mg of thiamine hydrochloride (C12H17ClN4OS.HCl), 7600mg-8400mg of cane sugar (C12H22O11), 7600mg-8400mg of mannitol, 6650mg-7350mg of agar and the balance of water. By adopting the culture medium disclosed by the invention, the potato tissue cultured seedlings grow slowly and need 140-150 days to grow to a height of 8cm since the inoculation date, thus the preservation time of the potato seedlings is remarkably prolonged.

Description

technical field [0001] The invention relates to a culture medium, in particular to a culture medium for delaying the growth speed of potato tissue culture seedlings. Background technique [0002] At present, one of the ways to preserve potato core seedlings at home and abroad is to use tissue culture, and MS medium is generally used for preservation, but the tissue cultured seedlings grown on MS medium need to be cut after about 30 days Transplanting once requires a lot of labor and materials, and such frequent operations will accelerate the physiological age of the core seedlings, which is not conducive to long-term storage. Some researchers also add plant growth regulators such as abscisic acid, penicillin, chlormequat, paclobutrazol, and B9 to MS medium to slow down the growth rate of tissue culture seedlings, but the storage time range is generally about 60 days to 90 days. Contents of the invention [0003] The purpose of the present invention is to overcome the defi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张新永包媛媛宋雷
Owner YUNNAN AGRICULTURAL UNIVERSITY
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