The invention discloses a cultivation method of mycorrhizal
edible fungus symbiotic seedlings, and relates to the technical field of
edible fungus cultivation, and the cultivation method specifically comprises the following steps: S1, culturing symbiotic
plant seedlings; s2,
mycelium culture of the mycorrhizal edible fungi: S21,
mycelium culture; s22, performing purification culture: selecting the hyphae in the step S21, inoculating the hyphae into a new
solid culture medium, and performing standing culture at 24-26 DEG C until the hyphae are overgrown; s23,
fermentation culture: inoculating the
hypha in the step S22 on a liquid
fermentation culture medium, and culturing on a constant-temperature shaking table at 25 DEG C to obtain a liquid strain; s3, infection: injecting the liquid strain in the step S23 into the symbiotic
seedling composite culture medium in the step S13, placing the symbiotic
seedling composite culture medium in an illumination
incubator for culture, and regularly supplementing a
nutrient solution during culture; and culturing for 60-90 days to form the mycorrhizal
edible fungus symbiotic
seedling. The method is high in
mycorrhiza infection rate, high in symbiotic seedling
survival rate and suitable for popularization.