21results about How to "Grow vigorously" patented technology

The invention provides a phellinus igniarius strain rich in polysaccharide and total flavone as well as a cultivation method and application thereof, relates to the technical field of biology, and aims to solve the problems that a phellinus igniarius variety with high yield and high polysaccharide and total flavone content is lacked in the prior art, and an existing cultivation method is difficult to meet the effects of high yield and income increase. The phellinus igniarius strain disclosed by the invention is phellinus igniarius, is named as Harmulberry No.1, and is preserved in China General Microbiological Culture Collection Center on October 31, 2024, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.41588. In the cultivation process of the phellinus igniarius sporocarp, a cultivar culture medium is prepared from, by mass, 78% of oak sawdust, 15% of trametes robiniophila cultivation waste, 5% of corn flour, 1% of gypsum powder and 1% of cane sugar. The phellinus igniarius variety disclosed by the invention has potential development and application values and can be used for preparing health-care foods or medicines.

The invention provides a tissue culture method for seedlings of apple trees, and belongs to the technical field of apple tissue culture breeding, the tissue culture method comprises the following steps: 1) disinfecting seeds of apple trees, removing seed coats, inoculating embryos after cotyledons are cut into a germination culture medium for germination, and obtaining tissue culture seedlings; and (2) cutting adventitious buds from the tissue culture seedlings, inoculating the adventitious buds into a proliferation culture medium, and carrying out proliferation culture to obtain seedlings. According to the tissue culture method, the embryos are used as explants, the embryos have better differentiation and propagation capacity, the regeneration capacity of the obtained tissue culture seedlings is higher than that of the tissue culture seedlings obtained by using other materials as explants, and the propagation coefficient is high.

The invention belongs to the technical field of biological seedling culture, and particularly relates to a seedling culture method with high survival rate, which comprises the following steps: collecting agricultural wastes such as rice husk, wheat straw, corn straw and the like, performing crushing and fermentation primary treatment, performing disinfection treatment on the treated agricultural wastes, and killing pathogenic microorganisms and pests in the agricultural wastes; a proper amount of wood chips, coco coir, compost and other biomass materials are selected, the prepared agricultural waste and biomass materials are mixed according to a certain proportion, then beneficial microorganisms and natural organic additives are added, the mixed substrate is subjected to stacking fermentation, and finally the substrate is used for the seedling raising process of nursery stocks. The air permeability and the water-retaining property of the substrate can ensure healthy growth of seedling root systems, and stable nutrition supply is provided. The fertility, the air permeability and the biological activity of the substrate are optimized, the survival rate and the growth speed of seedlings are remarkably increased, and the seedling raising period is shortened.

The invention discloses a C4 epimerase whole cell breeding method and a D-tagatose preparation method. C4 epimerase whole cells obtained through breeding grow robustly, are excellent in performance and are more suitable for recombinant escherichia coli for production; when the whole cell is used for producing D-tagatose, a whole cell culture does not need to be crushed, and other raw materials, catalysts and the like do not need to be added; the method has the advantages of simple reaction conditions, high efficiency, single reaction, few side reactions, easiness in control and industrial production.

The invention relates to the technical field of agriculture, in particular to a pelleting formula of isatis tinctoria seeds capable of resisting root diseases and promoting rooting. The formula comprises the following components: isatis tinctoria seeds, an antifungal agent, a rooting accelerant, a humectant, a nutrition additive and an adhesive. The pelleting formula is prepared by the following steps: firstly, uniformly mixing isatis tinctoria seeds with an antifungal agent; adding a rooting accelerant, and continuously stirring; adding the humectant, and fully mixing; adding a nutrition additive, and uniformly mixing with the seeds; adding an adhesive, and continuously stirring; feeding the mixture into a granulator for granulation to form seed pellets with the diameter of 3-5mm; and drying the prepared seed pellets, and packaging after quality inspection. According to the isatis tinctoria seed pelleting formula capable of resisting root diseases and promoting rooting provided by the invention, the disease resistance and rooting rate of the seeds are remarkably improved by optimizing formula components and a preparation process.

The invention aims to provide a vertical stony matrix planting method, which is realized by adopting the following technical scheme: preparing a vertical stony matrix, punching stone holes and filling wood plugs, planting dendrobium seedlings, managing and maintaining moisture, and finally expanding and harvesting root systems, after about 10 months, the dendrobium root systems are tightly wound on stones, and at the moment, a binding belt can be cut off, so that the dendrobium can grow naturally. After five years, old stems can be selected every year, new buds are left for continuous growth, and sustainable utilization is achieved. The dendrobium huoshanense (dendrobium huoshanense) planting method based on the vertical stone matrix has the remarkable beneficial effects that the yield and quality of dendrobium huoshanense are improved, sustainable development of the traditional Chinese medicine industry is promoted, and wide application prospects and popularization value are achieved.

The present application belongs to the technical field of millet planting, and particularly relates to a method for preventing and treating millet white hair disease, comprising the following steps: S1, spraying fungicides to a planting area in January; S2, spraying fungicides to the planting area again in February-March; S3, carrying out high-temperature sterilization on the planting area 10-15 days before millet sowing; S4, sowing millet in the planting area during the sowing period; and S5, spraying fungicides to the leaf surface of millet when the millet grows to the 7-leaf stage. The present application forms a synergistic prevention and treatment method by controlling the time node of fungicide spraying and cooperating with high-temperature sterilization. The prevention and treatment method has a significant prevention effect and strong radical treatment, can eliminate pathogenic bacteria in the soil from the root cause, greatly reduces the incidence of millet white hair disease, and significantly reduces the yield loss of millet.

The invention discloses a method for inducing mature embryos of Siberian apricot to breed seedlings, comprising the following steps: explant disinfection: disinfecting apricot kernels with seed shells removed and peeling off the seed coat to obtain mature embryos; sterile seedling induction: culturing mature embryos in sterile seedling induction medium to obtain sterile seedlings; sterile seedling proliferation: culturing sterile seedlings in sterile seedling proliferation medium to obtain sterile seedlings and strong seedlings; inducing callus: culturing rectangular leaves cut from sterile seedlings and strong seedlings in callus induction medium to obtain callus; differentiating cluster buds: transferring callus to cluster bud differentiation medium to obtain cluster buds; cluster bud subculture proliferation: culturing cluster buds in cluster bud subculture medium after cutting to obtain cluster bud strong seedlings; cluster bud rooting: cutting cluster buds into single buds and transferring them to rooting medium for rooting culture. The invention can solve the technical problems of long breeding cycle, low survival rate and poor breeding stability of Siberian apricot.

The invention discloses a low-temperature-resistant hericium erinaceus strain rich in triterpenes and proteins and a cultivation method of the low-temperature-resistant hericium erinaceus strain, relates to the field of biology, and aims to solve the problems that in the prior art, a hericium erinaceus variety which is high in protein yield, high in triterpenes yield, resistant to low temperature and good in commodity is lacked, and an existing cultivation management mode is difficult to meet the high-yield and income increasing effects. The invention provides hericium erinaceus QAHT-2 which is preserved in the China General Microbiological Culture Collection Center on January 3, 2023, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.40372. The invention further provides a preparation method of the hericium erinaceus QAHT-2. The hericium erinaceus QAHT-2 has the advantages that the hericium erinaceus QAHT-2 can be used for preparing the hericium erinaceus QAHT-2; the hericium erinaceus strain QAHT-2 is obtained through separation and domestication, and hyphae of the hericium erinaceus strain QAHT-2 grow fast and are robust; the low-temperature environment adaptability is high, fruiting can be achieved at the temperature of 10-25 DEG C, and fruiting bodies still present snow white in the low-temperature environment. The fruiting body of the hericium erinaceus strain provided by the invention has higher protein content and higher triterpenoid content, and has higher edible and medicinal values.

The invention discloses a growth conditioning method for waxberry fusarium wilt prevention and control. A conditioning agent is researched and developed to condition a waxberry growth soil environment, promote root growth, improve stem and leaf growth, improve the biological and environmental stress resistance of waxberries, control the occurrence of waxberry fusarium wilt and realize sustainable high-yield and high-quality production. The growth conditioner comprises the following components: 60 parts of a fermented cow dung mixture, 20 parts of fermented hickory hull and 20 parts of calcium carbonate, wherein 5 kilograms of compound fertilizer, 3 kilograms of calcium magnesium phosphate fertilizer and 5 kilograms of potassium sulfate fertilizer are added per cubic meter; the compound fertilizer is selected from NPK (Nitrogen, Phosphate and Ketone) 16-6- The growth conditioner provided by the invention can regulate the pH value of soil, the pH value of waxberry site soil can be increased from 4-5 to 5.5-6, the organic matter content and air permeability of the soil are improved, the water and fertilizer retention capability is enhanced, and the microbial activity is promoted.

This invention belongs to the field of plant tissue culture technology, specifically relating to a tissue culture rapid propagation medium and method for rooting *Carex latifolia*. The invention provides a tissue culture rapid propagation medium for rooting *Carex latifolia*, comprising a rooting medium; the rooting medium uses 1 / 2 MS as the basal medium and further includes NAA 0.8 mg·L⁻¹, IBA 1.0 mg·L⁻¹, sucrose 30 g·L⁻¹, and agar 7 g·L⁻¹, with a pH of 5.8-6.0. The invention also provides a method for direct rooting of *Carex latifolia* through tissue culture rapid propagation. Aseptic seedlings of *Carex latifolia* have their outer, weaker leaves removed, and the original root system cut off, retaining 2-3 cm of the above-ground portion. These seedlings are then inoculated into the rooting medium for rooting culture to obtain rooted seedlings. After hardening off the rooted aseptic seedlings, the well-rooted *Carex latifolia* seedlings are transplanted into a substrate to form new, complete plants. The method for rapid propagation of broadleaf sedge by tissue culture provided by this invention has the characteristics of high rooting rate, large average number of roots, well-developed root system, high transplant survival rate, and robust regenerated seedlings.

The invention discloses a method for improving seed germination under a salt stress condition and application thereof, and belongs to the technical field of crop production. According to the method provided by the invention, the germination ability of the seeds under the salt stress condition can be remarkably improved, the germination rate, germination potential and germination index of the watermelon seeds and the muskmelon seeds under the salt stress condition simulated by 250mM NaCl can be remarkably improved by soaking the seeds with 10 mu M methyl caffeate, and the germination of the watermelon seeds and the muskmelon seeds under the normal condition is not influenced; seedlings generated by the seeds treated by the method provided by the invention grow robustly, and compared with seedlings grown under normal conditions, obvious abnormity is not observed, so that the method provided by the invention is safe and has no toxic effect on plant growth; the provided method is simple, effective and low in cost, and can be used for improving the seed germination capacity under the salt stress condition so as to improve the utilization capacity of the saline-alkali soil and improve the crop yield and quality contribution of the saline-alkali soil.

The invention belongs to the technical field of plant propagation, and discloses a rare Chinese herbaceous peony bud grafting rapid propagation method, which comprises the following steps: (1) rootstock cultivation and selection, (2) planting box treatment, (3) bud grafting knife treatment, (4) bud scion collection and treatment, (5) bud grafting operation process, (6) management after bud grafting, and (7) bud grafting seedling field planting. According to the growth characteristics of the rare Chinese herbaceous peony, Chinese herbaceous peony bud grafting is adopted for rapid propagation, the cultivation method is optimized, and rapid popularization of high-quality varieties is improved. Meanwhile, a stock selection and cultivation method is optimized, local peony seedlings are selected as stocks, affinity is good, stress resistance is high, and a good foundation is provided for bud scion healing and growth; meanwhile, through normative matrix proportion and cultivation management, it is ensured that the stock grows robustly, and the budding survival rate is increased.

The invention discloses a cultivation method of mycorrhizal edible fungus symbiotic seedlings, and relates to the technical field of edible fungus cultivation, and the cultivation method specifically comprises the following steps: S1, culturing symbiotic plant seedlings; s2, mycelium culture of the mycorrhizal edible fungi: S21, mycelium culture; s22, performing purification culture: selecting the hyphae in the step S21, inoculating the hyphae into a new solid culture medium, and performing standing culture at 24-26 DEG C until the hyphae are overgrown; s23, fermentation culture: inoculating the hypha in the step S22 on a liquid fermentation culture medium, and culturing on a constant-temperature shaking table at 25 DEG C to obtain a liquid strain; s3, infection: injecting the liquid strain in the step S23 into the symbiotic seedling composite culture medium in the step S13, placing the symbiotic seedling composite culture medium in an illumination incubator for culture, and regularly supplementing a nutrient solution during culture; and culturing for 60-90 days to form the mycorrhizal edible fungus symbiotic seedling. The method is high in mycorrhiza infection rate, high in symbiotic seedling survival rate and suitable for popularization.

The present invention discloses a cold-region hydrangea strain and a high-yield cultivation method thereof, which relates to the field of biotechnology and aims to solve the problem that there is a lack of high-yield hydrangea varieties suitable for cold-region cultivation in the prior art. Sparassis crispa ) Haxiu 3 was deposited in the General Microbiology Center of China Microorganism Culture Collection Committee on June 20, 2024. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No. 41342. The hydrangea fungus Haxiu 3 obtained by separation and domestication in the present invention has the advantages of short mycelium growth time, higher primordium formation rate, and high yield. It can have a higher mushroom production rate in the temperature range of 10~25℃, has stronger adaptability to the environment, has good low temperature resistance, and is more suitable for cultivation in cold areas. Haxiu 3 of the present invention is highly sensitive to light. Irradiation with yellow light during the primordium differentiation period can promote primordium formation, shorten the primordium formation time, and improve the uniformity of mushroom production.

The present application relates to the technical field of plant tissue culture, and provides a tissue culture method for promoting germination of Zingiber zerumbet Smith, wherein Zingiber zerumbet Smith tubers are used as explants, and after being subjected to disinfection treatment, the explants are inoculated into an induction medium, a proliferation medium and a rooting medium in sequence, and then are subjected to acclimatization, seedling raising and transplanting, so that finished Zingiber zerumbet Smith seedlings are obtained. The germination rate, the proliferation coefficient and the rooting rate of the Zingiber zerumbet Smith tubers can be significantly improved by using the culture media which are optimized and adjusted in the technical scheme of the present application. In addition, the reasonable application of foliar fertilizer and fungicide after transplantation in the present application can further improve the growth vigor and the disease resistance of Zingiber zerumbet Smith seedlings, and ensure the survival rate of the seedlings, thereby providing reliable technical support for rapid propagation and large-scale planting of Zingiber zerumbet Smith.

The invention relates to the technical field of forest tree genetic breeding and propagation, and particularly discloses a seedling raising method for improving the grafting survival rate of phoebe bournei. Comprising the following steps: S1, cultivating phoebe bournei cutting seedlings as rootstocks, and keeping the rootstocks in a robust state for later use; s2, semi-lignified branches growing in the current season are collected from the excellent phoebe bournei stock plants to serve as scions; s3, performing disinfection and pretreatment on the scions to prepare scion sections with wedge-shaped notches; s4, cutting off and cutting the stock, and combining the scions treated in the step S3 with the stock to enable cambiums to be aligned; s5, sealing and fixing the grafting joint part by using a binding belt material; s6, performing stage moisturizing and light and temperature regulation and control on the grafted plants. The seedling raising method can be applied to the fields of efficient germplasm resource utilization, artificial forest and landscape forest construction and the like, and has the advantages that the grafting survival rate is high and stable, the interface healing speed is high, the quality is good, and nursery stocks grow robustly and neatly.

The invention relates to the technical field of plant cultivation, in particular to an excellent kiwi fruit rootstock breeding method which comprises the steps that actinidia kolomikta which grows robustly and vigorously is selected as a male parent material, and actinidia arguta which grows robustly and vigorously is selected as a female parent material; hybridizing the male parent material and the female parent material to obtain hybrid seeds; carrying out seedling sowing, seedling cultivation and seedling field planting on the hybrid seeds; selecting an excellent kiwi fruit plant from the planted seedlings as a kiwi fruit rootstock material; the kiwi fruit rootstock material is used for rapid breeding, and the novel woody-root kiwi fruit rootstock which is resistant to drought and waterlogging and strong in growth is obtained. By means of the breeding method, the excellent novel woody-root kiwi fruit stock which is resistant to drought, resistant to waterlogging and strong in growth can be obtained.

The invention provides a method for improving the pod bearing rate of butterfly orchid cross pollination, which comprises the following steps: preparation before pollination: selecting a female parent and a male parent to construct a cross combination, selecting a butterfly orchid plant as a pollination object, and preparing tools and equipment required by pollination; a pollination process is optimized, wherein a pollination material is selected, and pollination is conducted 1d-30d after the butterfly orchid flowers bloom; hanging a tag on the female parent after pollination; performing repeated pollination according to the blooming degree of the flowers and the pollination condition; cultivation management after pollination: after pollination, controlling day and night temperature difference, air humidity and illumination intensity, and providing an optimal growth environment for phalaenopsis amabilis; according to the growth requirements of the phalaenopsis, fertilization, watering and pest control are conducted, and healthy growth of plants is promoted. The pollination pod bearing rate reaches 95% or above, and the technology is simple and beneficial to popularization.

The invention discloses a plant temperature stress resistance gene RAD and application thereof, relates to the field of molecular biology, and in particular relates to a plant temperature stress resistance gene and application thereof in improving temperature applicability and increasing crop yield. The invention provides a nucleotide sequence of the temperature stress resistance gene RAD and a coded protein sequence, the gene can obviously enhance the temperature stress resistance of plants and improve the growth condition of the plants, and a certain theoretical basis and a new gene resource are provided for the breeding of temperature stress resistance varieties of the plants.

The invention discloses a complete method for improving soil and supplementing various elements lacked by plants in a full-ecological mode. The complete method comprises the following steps that 1, organic fertilizer is prepared; 2) preparing an element fertilizer, and mixing the element fertilizer with an organic fertilizer to obtain a mixture; 3) changing the structure of the soil at a certain depth to loosen the soil, and spreading the mixture on the surface of the soil for mixing; and 4) preparing a microbial agent, and spraying the microbial agent to the surface of the soil.