86 results about "Aphidius gifuensis" patented technology

The present invention provides an aphidius gifuensis scale breeding technique, and belongs to the branch of technology of biological control of agricultural destructive insects for solving problems that the prior breeding technique of aphidius gifuensis is inefficiency and adj. industrialization is not liable to realize.The invention provides a aphidius gifuensis scale breeding technique and the embodiment scheme of the present invention comprises the following steps:(a) tobacco plant replanting: when growing to a resettling growth stage, the tobacco plants are replanted to a honeybee breeding booth;(b) myzus inoculation to tobacco plant: myzus is inoculated to the tobacco plant when the tobacco plant grows out 9-12 real leaves, and each tobacco plant is inoculated with 20-30 myzuses;(c) honeybee inoculation in parasitism: when myzuses are 2000-3000 per tobacco plant, artificial inoculation is performed, after 10-14 days, deadlocked myzuses are formed, and then after a week, the deadlocked myzuses will become aphidius gifuensis.By using the scale breeding technique of the present invention, each plant can obtain more than 8000 eadlocked myzuses, thus, the breeding cycle is shortened, and large-scale production can be performed.

The invention relates to an overwintering preserving breeding method for an aphidius gifuensis and belongs to the technical field of biological control. The overwintering preserving breeding method for the aphidius gifuensis solves the problem that in the prior art, overwintering preservation is performed on the aphidius gifuensis difficultly, and the heavy demand for the aphidius gifuensis in a tobacco planting season in a coming year is difficult to meet. The overwintering preserving breeding method comprises a first step of greenhouse aphidius gifuensis selection which comprises cultivation of host plants, collecting of an aphidius gifuensis source, aphidius gifuensis inoculation in a greenhouse, and purification and rejuvenation; a second step of winter greenhouse aphidius gifuensis preservation which comprises cultivation of aphidius gifuensis preserving plants, aphidius gifuensis inoculation in the greenhouse, and purification and rejuvenation; and a third step of expanding propagation in the greenhouse. The aphidius gifuensis is successfully accumulated and preserved during the time from finish of massive reproduction of the aphidius gifuensis in August to April in a coming year and before massive reproduction of the aphidius gifuensis, and the aphidius gifuensises with enough quantity and good quality are provided for massive reproduction of the aphidius gifuensis in a next year.

The invention provides a method for overwintering species conservation propagation of myzus persicae and belongs to the technical field of prevention and control of aphidius gifuensis. Aiming to solve the problem that the overwintering species conservation of the myzus persicae cannot be realized so that the huge demand for the aphidius gifuensis in the next tobacco planting season is difficultly met in the prior art, the invention provides the method for the overwintering species conservation propagation of the myzus persicae, which comprises the following steps of: a, collecting the myzus persicae; b, inoculating the myzus persicae to tobacco seedlings; c, removing toxins from the myzus persicae; d, purifying and rejuvenating the myzus persicae; e, performing the overwintering species conservation of the myzus persicae; and f, enlarging the propagation of the myzus persicae. The method has the advantages of successfully realizing the large-scale propagation of the myzus persicae, ensuring quality and quantity of the young myzus persicae, providing enough high-quality myzus persicae for the large-scale propagation of the aphidius gifuensis in next year, and creating better ecological benefit, economic benefit and social benefit.

The invention provides a method for propagating Aphidius gifuensis by pea aphids. The method provided by the invention utilizes two facilities including a seedling culture big shed and a field small shed and takes broad beans and peas as host plants for propagating aphids; the method takes the pea aphids as host aphids for propagating Aphidius gifuensis and then the propagated Aphidius gifuensis are released to a large-scale tobacco field to kill the aphids on tobacco leaves. The method selects the broad bean seeds and the pea seeds and then tender green bean plants are cultivated in a big greenhouse or the small shed built in the fields; the pea aphids are inoculated on the bean plants and then the pea aphids are propagated; then the Aphidius gifuensis is inoculated on the tender bean plants which is propagated with a lot of pea aphids and then the Aphidius gifuensis is propagated. Then a lot of propagated Aphidius gifuensis are released into the large-scale tobacco field to kill theaphids on tobacco leaves. Finally, the aim of biologically preventing and treating aphids is realized and the pesticide pollution caused by preventing the aphids is avoided.

The invention relates to a method for breeding and feeding Aphidius gifuensis Ashmesd in a floating seedling raising shed and belongs to the field of insect feeding. According to the biological and ecological behaviors of the Aphidius gifuensis Ashmesd and the modern agricultural science and technology, by using the existing floating seedling raising shed and the time difference between Myzus periscae (Sulzer) breeding period and the Aphidius gifuensis Ashmesd larva growth and development period, breeding Aphidius gifuensis Ashmesd required for Aphidius gifuensis Ashmesd breeding is obtained at the same time when the Myzus periscae (Sulzer) is bred. The method for breeding and feeding the Aphidius gifuensis Ashmesd in the floating seedling raising shed has the advantages that the operation is simple to conduct, the cost is low, the repetitive investment is not required, the survival rate is high, the scale breeding is facilitated and the popularization is facilitated. Compared with the method for breeding and feeding the Aphidius gifuensis Ashmesd in a seedling raising greenhouse, on the premise that the bred and fed quantity is not affected, the cost per mu is reduced by RMB4.5, the quantity of bred and fed adult Aphidius gifuensis Ashmesd and the parasitism rate are respectively improved by 21.3 percent and 3.8 percent, the number of parasitic aphids on each plant in the field and the control rate of the Aphidius gifuensis Ashmesd to the aphids are improved by 4.75 percent, the economic benefit, the social benefit and the ecological benefit are good, and a good foundation is laid for the large-scale breeding and feeding of the Aphidius gifuensis Ashmesd.

The invention discloses an industrial cultivation method for aphidius gifuensis ashmead. The method comprises the following steps: cultivating host plants for myzus persicae sulzer, and breeding myzus persicae sulzer on the host plants; breeding mummified aphids of aphidius gifuensis ashmead on the myzus persicae sulzer by taking the bred myzus persicae sulzer as hosts for breeding aphidius gifuensis ashmead; separating the bred mummified aphids from leaves of the host plants, and collecting the mummified aphids; sterilizing the collected mummified aphids, performing air-drying treatment, and performing irradiation treatment with ultraviolet light; rejecting the mummified aphids which are diseased, damaged and smaller, and transferring the mummified aphids into sub-packaging test tubes or uniformly spreading the mummified aphids on paper cards to form mummified aphid cards; storing the sub-packaging test tubes containing the mummified aphids or the mummified aphid cards, and performing packaging according to requirements. According to the method, the cultivation cycle of aphidius gifuensis ashmead is shortened, the collection, the storage and the transportation of aphidius gifuensis ashmead are integrated, the utilization rate of aphidius gifuensis ashmead is increased, and aphidius gifuensis ashmead tends to be developed towards commercial and industrial production.

The invention discloses a nutrient substance for prolonging the service life of adult bees of aphidius gifuensis ashmead. The nutrient substance is composed of 25% of white sugar, 5% of cane sugar, 5% of glucose, a small number of mineral substances, vitamins, amino acid and copper gluconate and the balance water. The nutrient substance can be supplied to aphidius gifuensis ashmead in the field with aphidius gifuensis ashmead, can provide sufficient nutriment for the growth and development of adult bees of aphidius gifuensis ashmead, prolongs the service life of adult bees of aphidius gifuensis ashmead, improve the activity and oviposition capacity, is low in cost, and guarantees the large-scale propagation and application of aphidius gifuensis ashmead. The aphidius gifuensis ashmead is fed artificially and released in the field, the service life of myzus persicae is shortened and the fecundity of myzus persicae is decreased by more than 90% through oviposiiton and parasitism of female bees on myzus persicae, and the myzus persicae control function is remarkable. The service life of adult bees of aphidius gifuensis ashmead is prolonged, the survival rate of adult bees of aphidius gifuensis ashmead is improved, and large-scale application of aphidius gifuensis ashmead is facilitated.

The invention relates to the field of tobacco pest control, in particular to an automatic aphidius gifuensis pupa quantitative packaging machine. The automatic aphidius gifuensis pupa quantitative packaging machine consists of a rack, an electrical controller, an air pump, a negative pressure air valve, a positive pressure air valve, an air pipe, a support, a bearing pedestal, a blowing-suction roll, a blowpipe, a micro motor, a storage box, a feeding box, a numerical control sliding table and an aphidius gifuensis pupa hopper. Automatic quantitative packaging before aphidius gifuensis pupa seedlings are released can be realized automatically, and the production efficiency is improved. In addition, according to the characteristic that aphidius gifuensis pupas are delicate and vulnerale, positive pressure air and negative pressure air are adopted for quantitative packaging and storage of the aphidius gifuensis pupas, the aphidius gifuensis pupas can not be harmed by machines in a packaging and storage process, and the aphidius gifuensis pupa seedlings serve for aphid control work better.

The invention relates to an adult bee collecting method of aphidius gifuensis bred in an arch shed. According to the method, an insect prevention net (5) is integrally covered outside a shed frame of the arch shed, a shade net (6) is covered at the arch top of the arch shed, the top of the phototropic surface of the arc shed is provided with at least one bee collecting hole (8), a detachable bee collecting bag (2) is connected outside the bee collecting hole, the negative geotropism and the phototaxis of the aphidius gifuensis are utilized, adult bees of the aphidius gifuensis bred and survived in the arch shed are collected into the bee collecting bag (2). The adult bee collecting method has the characteristics that the characteristic of aphidius gifuensis bred in a small shed is combined, the negative geotropism, the phototaxis habit and the activity peak time of the aphidius gifuensis are utilized, the bee collecting bag is used for collecting adult bees for releasing in fields, the dying of adult bees in the shed before field loose keeping is prevented, and the adult bees are effectively utilized.

A method of breeding aphidius gifuensis in high-density, in a breeding cote the number of nutrition carriers is about 600 plants in per square meter area, 20 days after sowing and seedling, inoculating the tobacco aphid when the seedling is young, then another 10 days later, inoculating the aphidius gifuensis, about another 15 days apis appears. high-density breeding technology apis feeding cycle of the aphidius gifuensis is 45 days, which can reduce 50 days corresponding to individual apis feeding technology, the aphid feeding amount is 11.2 ten thousand/m[2], the benefit is improved 100.7%, the apis feeding amount is 2.8 ten thousand/m[2], the benefit is improved 60%.

The invention discloses a whole-course biological control method of tobacco diseases and insect pests. The method comprises the following steps: 1) in the seedling stage: using predatory mites for controlling thrips and tomato spotted wilf viruses; using harmonia axyridis and aphidius gifuensis for controlling myzus persicae and the thrips; using penicillium to inactivate mycelia, and 0.5% of chitosan and 0.5% to 2% of amino-oligosaccharin for controlling tobacco mosaic diseases; 2) in the field period: using the predatory mites and harmonia axyridis for controlling the thrips, the tomato spotted wilf viruses and the myzus persicae; using sex attractants for controlling prodenia litura adults and helicoverpa assulta adults; using Cahtheconidea furcellata for controlling prodenia litura larvae; using 0.5% of chitosan and 0.5% to 2% of amino-oligosaccharin for controlling the tobacco mosaic diseases; using Holder Burkholderia, and Pythium oligandrum for controlling tobacco black shank, black root rot, root knot nematode diseases and the like. The whole-course biological control method of the tobacco diseases and insect pests disclosed by the invention thoroughly eliminates pollution of chemical pesticides on the environment and tobacco pesticide residues and effectively controls the diseases and insect pests continuously and effectively, and the loss of tobacco quality for the diseases and insect pests is controlled within 5%.

The invention discloses a method for regulating diapause of aphidius gifuensis ashmead. The method provided by the invention comprises following step 1) or 2), wherein the step 1) comprises the following steps of: A, treating aphidius gifuensis ashmead larva at 5-15 DEG C and with a thermoperiod being C18: T6 and a photoperiod being L8: D16 so as to obtain the aphidius gifuensis ashmead in a diapause state; B, carrying out diapause termination on the aphidius gifuensis ashmead in the diapause state to obtain hatched aphidius gifuensis ashmead; and the step 2) comprises the following steps of: C, carrying out diapause treatment on the aphidius gifuensis ashmead larva at a low temperature and with a photoperiod being L8: D16 to obtain the aphidius gifuensis ashmead in the diapause state, wherein the low temperature is no more than 12 DEG C; and D, carrying out diapause termination on the aphidius gifuensis ashmead in the diapause state to obtain the hatched aphidius gifuensis ashmead. The experimental results of the invention show that the method provided by the invention can realize accurate regulation on the development progress of the aphidius gifuensis ashmead.

The invention discloses an overwintering breeding method for an aphidius gifuensis population. The method comprises the following steps that 1, host plant breeding; 2, aphid inoculation; 3, aphid breeding; 4, aphidius gifuensis inoculation; 5, aphidius gifuensis breeding; 6 reproduction expanding. In a greenhouse with controllable environment conditions, alligator alternanthera herbs are planted, aphidius gifuensis is bred for overwintering, the characteristic that the aphidius gifuensis survives and propagates easily is fully utilized, it can be ensured that the aphidius gifuensis is supplied on a large scale all year round, the aphidius gifuensis can be produced and bred continuously and circularly by means of the technology, the defects that it is difficult to preserve the aphidius gifuensis in an overwintering mode by means of the aphidius gifuensis breeding technology, the requirement for loose keeping in a tobacco field next year can not be met are overcome, and the industrialization development target of the biological prevention and control technology from laboratories to field application and popularization is achieved.

The invention discloses a Myzus periscae overwintering breed conservation breeding method including the following steps of: (1) performing collection, detoxification, purification and renovation on Myzus periscae, and obtaining robust and disease-free Myzus periscae; (2) inoculating the Myzus periscae on fresh radish sprouts or green vegetable sprouts in a glass greenhouse for breeding in November every year; (3) inoculating the Myzus periscae which is on the fresh radish sprouts or green vegetable sprouts onto tobacco flowers in the glass greenhouse in February of the second year, and breeding the Myzus periscae on the tobacco flowers for 10-15 days; and (4) inoculating the Myzus periscae which is on the tobacco flowers onto bottom leaves of tobacco plants in a plastic shed and performing expanding propagation on the Myzus periscae. According to the invention, the tobacco flowers are used as transitional hosts. The tobacco flowers are the parts of the tobacco plants with the lowest nicotine content. The tobacco flowers contain honeydew, and attract the Myzus periscae to eat, and thus the activity and fertility of the Myzus periscae can be ensured. After the method of the invention is used, the survival rate of the Myzus periscae inoculated onto the tobacco leaves is raised to 70% from 10% in the prior art. The egg laying amount, after the Myzus periscae is inoculated with aphidius gifuensis for 7 days, is 60-70 times of the egg laying amount in the prior art.

The invention discloses a green preventing and control method for tobacco diseases and insect pests. The green preventing and control method comprises the following steps of (1) soil improving and sanitation of a tobacco land; (2) nitrogen control and densifying of the tobacco land; (3) propagation and releasing of aphidius gifuensis; (4) arranging of an insect trapper on the tobacco land; (5) spraying of biological pesticide. The green preventing and control method has the advantages that by combining the technical means of improving soil of the tobacco land, performing nitrogen control and densifying technologies on the tobacco land, releasing the aphidius gifuensis, arranging the insect trapper, and spraying the biological pesticide, the green preventing and control purpose on the diseases and the insect pests is realized in the tobacco planting process, the usage amount of chemical pesticide is greatly reduced, the influence to the quality of tobacco leaves by the chemical pesticide is reduced, and the pollution to environment is reduced.

The invention discloses a method for breeding aphidius gifuensis ashmeads through alternanthera philoxeroides. The method includes the following steps that 1, yellow-green type tobacco aphids are collected, and aphid species are bred after detoxification purification is conducted; 2, aphidius gifuensis ashmeads are collected, and bee species are bred after purification is conducted; 3, a breeding chamber is prepared, and alternanthera philoxeroides are cultured; 4, yellow-green type tobacco aphids are inoculated and bred; 5, inoculation is conducted on the aphidius gifuensis ashmeads, and mummified aphids are bred; 6, the mummified aphids of the aphidius gifuensis ashmeads or adult bees are collected. According to the method for breeding the aphidius gifuensis ashmeads through the alternanthera philoxeroides, the features that the alternanthera philoxeroides survive easily, breed easily and grow in four seasons are fully utilized to replace traditional tobacco, cabbages and the like to serve as carriers to breed the aphidius gifuensis ashmeads, so that the aphidius gifuensis ashmeads can be bred in the four seasons, reasonable utilization of agricultural resources is achieved, and the breeding cost is reduced; meanwhile, the aphidius gifuensis ashmeads are bred in batches, multi-generation repeated reproduction of the aphidius gifuensis ashmeads is avoided, the number of hyper-parasitoids of the aphidius gifuensis ashmeads is reduced, and the field survival ability of the aphidius gifuensis ashmeads is increased.

The invention discloses an industrial production method for aphidius gifuensis products. The method includes steps: step (1), purifying after natural culturing and rejuvenation of breeding aphids and breeding wasps; step (2), adopting large-aperture plug seedling cultivation of big seedlings for mass propagation of breeding aphids and breeding wasps; step (3), adopting small-aperture floating trays for cultivation of high-density tobacco seedlings to increase seedling amount in unit area, adopting special large-aperture wasp propagation floating trays for cultivation of strong tobacco seedlings and wasp propagation, and performing inoculation of the breeding wasps in a high proportion when the average aphid amount reaches a certain quantity; step (4), collecting and packaging aphidius gifuensis. By the industrial production method, the problem of epidemic diseases in high-density wasp propagation, the problem of basic facility insufficiency and problems of aphidius gifuensis formation uniformity, collection, storage and long-distance transport are solved; existing inflexible time limitation in wasp propagation and release is broken, a system aphidius gifuensis industrial production technique is formed, and the aphidius gifuensis products which meet industrial production requirements of availability for large-scale production, quality stability, long-term storage and convenience in long-distance transport are obtained.

The invention discloses a method for realizing three-dimensional propagation of Aphidius gifuensis Ashmaed by means of a cuttage plant. The method comprises the following steps of 1) cultivating Gynura japonica as a host plant; 2) breeding aphids; 3) breeding the Aphidius gifuensis Ashmaed; and 4) collecting mummy aphids for manufacturing an Aphidius gifuensis Ashmaed egg aggregate. The method has advantages of short period, small space requirement and easy mummy aphid collection. The method can greatly reduce an Aphidius gifuensis Ashmaed propagation cost and can promote large-scale propagation and commercial production of the Aphidius gifuensis Ashmaed.

The invention provides artificial aphids for aphidius gifuensis ashmead propagation. The nutritional compositions for preparing artificial aphids include the following components in percent concentration by mass: 30 percent of aphid homogenate, 20 percent of egg yolk, 20 percent of skim milk, 10 percent of sucrose, 1 percent of inorganic salt solution, 2 percent of complex vitamin solution, 10 percent of complex amino acid solution, 0.1 percent of methyl p-hydroxybenzoate, 0.2 percent of agar and 6.7 percent of distilled water. The artificial aphids can effectively supply nutrition essential to development of aphidius gifuensis ashmead, and an attractant for oviposition of aphidius gifuensis ashmead is applied to the surface of artificial aphids, so that the survival rate, parasitism rate and eclosion rate of aphidius gifuensis ashmead can meet actual requirement. The artificial aphids can be used for solving defects of complex process, large occupation space, high cost and the like of a traditional propagation method, and the propagated aphidius gifuensis ashmead can be easily collected, stored and released; and the artificial aphids have the advantages of wide raw material source, simple preparation process, and is suitable for large-scale popularization.

The invention discloses a method for expanding propagation of aphidius gifuensises. The method comprises the following steps: inoculating to-be-spawned aphidius gifuensis queen bees to plant seedlings with the schizaphis graminums to conduct bee breeding, and collecting the aphidius gifuensises to achieve the expanding propagation. According to the method, the schizaphis graminums are used as bee breeding hosts, technical links of best inoculating ratio, inoculating time and aphidius gifuensis collection are adopted, and a parasitic wasp expanding propagation technique which is high-efficiency, economic and simple is formed through repeated explorations and improvements, the expanding propagation of a large number of aphidius gifuensises can be achieved rapidly, and the purpose of biological prevention and control for injurious insects is achieved.

The invention discloses a continuous aphidius gifuensis releasing method and device. The method comprises the steps that 1, a breeding shed is built in a tobacco field, and a flue-cured tobacco variety K326 is cultivated in the shed; 2, when each tobacco plant cultivated in the shed grows out 8-10 effective leaves, the tobacco plants are inoculated with myzus persicae, and the myzus persicae is bred; 3, when the breeding quantity of myzus persicae on the tobacco plants in the shed reaches 2,500-3,000 per plant, the tobacco plants are inoculated with aphidius gifuensis according to the aphidius gifuensis quantity with the myzus persicae to aphidius gifuensis ratio of 50:1, and the aphidius gifuensis is bred through the parasitic myzus persicae; 4, the aphidius gifuensis can be continuously released through a aphidius gifuensis releasing opening formed in the breeding shed after being bred to achieve the purpose of preventing and controlling the condition that the myzus persicae harms the tobacco leaves, wherein the breeding shed comprises an arch-shaped framework and a pest-proof screen arranged on the arch-shaped framework in a covering mode, one end of the arch-shaped framework is higher than the other end, and the aphidius gifuensis releasing opening is formed in the top of the higher end of the arch-shaped framework and also covered with the pest-proof screen. Compared with the prior art, the method and device are particularly beneficial for breeding the myzus persicae, and meanwhile quick and continuous aphidius gifuensis releasing can be achieved.

The invention relates to an inset community monitoring evaluation method for a tobacco field after aphidius gifuensis ashmead is released in a large scale and belongs to the field of tobacco field ecology. The method includes the following steps of 1, determining a monitoring range and sample plants of the tobacco field by means of a random sampling method and a sample selection method; 2, according to direct and indirect influence relations between aphidius gifuensis ashmead and insets of the tobacco field, determining monitored objects; 3, according to the growth of tobacco plants and growthconditions of the insets, determining a monitoring method and monitoring time; 4, conducting monitoring evaluation, wherein according to monitoring indexes, a multi-index evaluation method is adoptedfor evaluation and analysis of the inset community of the tobacco field after aphidius gifuensis ashmead is released. The occurrence rate of myzus persicae in the tobacco field can be obtained, the density of the natural enemies of myzus persicae can be obtained, the density of main pests and the data indicating the diversity of the inset community can also be obtained, the ecological effect of aphidius gifuensis ashmead which is released in the large scale within an application area is determined, and bases are provided for further improving the application of aphidius gifuensis ashmead.

The invention belongs to the technical field of biological prevention and control for agriculture and forestry pests, and particularly relates to a method for propagation of aphidius gifuensis for multiple times by using tobacco plants. The method comprises the following steps of breeding of myzus persicae host plants, propagation of the aphidius gifuensis for the first time, propagation of the aphidius gifuensis for the second time, propagation of the aphidius gifuensis for the third time, and propagation of the aphidius gifuensis for the fourth time. The method has the advantage that the developed root system of a tobacco plant is sufficiently utilized, and new leaves are promoted to germinate and quickly grow, so that the tobacco plant can be reutilized, the secondary seedling cultivation and transplanting can be avoided, the labor intensity is decreased, the time is saved, and the propagation efficiency of the aphidius gifuensis is greatly improved.

The invention discloses a method for industrially producing a aphidius gifuensis ashmaed product. The method includes the following steps that 1, aphidius gifuensis ashmaed is bred in a homogeneous and high-density mode; 2, aphidius gifuensis ashmaed mummified aphids are automatically collected; 3, packaging is conducted automatically. The aphidius gifuensis ashmaed product is produced, the aphidius gifuensis ashmaed breeding cost is reduced, the usage convenience of aphidius gifuensis ashmaed and the production efficiency are improved.

The invention discloses a localization method for breeding and releasing of aphidius gifuensis ashmead in the Shaanxi area. The method comprises the following steps of 1 myzus persicae sulzer host breeding, wherein an appropriate tobacco variety is selected for seedling raising and transplanting; 2 aphid screening and reproduction, wherein aphid species are collected for breeding and screening; 3 aphidius gifuensis ashmead reproduction through myzus persicae sulzer, wherein bees are collected, after copulation, the phidius gifuensis ashmead and the host myzus persicae sulzer natural breed, and mummified aphids are obtained; 4, bee releasing, wherein bee releasing operation is conducted in the initial stage of the myzus persicae sulzer occurrence, the frequency and quantity of bee releasing are determined according to the myzus persicae sulzer occurrence, mummified aphid seedlings can be placed, and adult bees can be directly released. The method has the advantages that according to the practical situation of the Shaanxi area, large scale of local aphidius gifuensis ashmead reproduction and releasing, the control cost of a tobacco grower for myzus persicae sulzer is reduced, and the quality of local tobacco leaves is improved.

The invention provides a method for rapid aphid propagation in an aphidius gifuensis breeding process. The method comprises the steps of culturing tobacco seedlings by using a large hole floating tray stereo greenhouse; vaccinating aphids by using 4-instar wingless young aphids when the tobacco seedlings grow to have four main leaves and one terminal bud each, 3 aphids for each seedling, and keeping the temperature in the greenhouse at 16-28 DEG C and the humidity at 52-54% after aphid vaccination. The light supplementing time of the stereo greenhouse is set to be 18:00-6:00 every day for a bright period, 6:00-8:00 for a bright-to-dark period, and 8:00-18:00 for a dark period; in the bright period, 3 groups of light supplementing is needed, the carbon dioxide is 1000-1200ppm and the temperature is 28 DEG C; in the bright-to-dark period, a skylight window is opened, a circulating fan is started and the temperature is 18 DEG C; in the dark period, the skylight window is opened and the temperature is kept at 16-18 DEG C. The method can guarantee that the quantity of crops in the greenhouse is suitable for the growth and propagation of aphids and guarantee that the temperature and the humidity at night are more suitable for crop growth and aphid propagation.