Artificial aphids for aphidius gifuensis ashmead propagation
A technology for Braconid aphid and aphids, which is applied to the field of insect reproduction, can solve the problems of occupying a large area of breeding sites, cumbersome artificial reproduction processes, and many links, etc., and achieves the effects of being suitable for large-scale promotion, convenient application and easy collection.
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Embodiment 1
[0024] Embodiment 1: the selection of nutritional composition components
[0025] According to the results of the analysis of the nutritional composition of Aphids, combined with the nutritional elements necessary for the growth and development of insects, the applicant selected the composition and ratio of the components of the nutritional composition, and determined the optimal one according to the eclosion rate of A. Components and ratios.
[0026] This embodiment lists representative three groups of nutrient combinations in the screening process for illustration:
[0027] The first group: egg yolk 35%, skimmed milk 35%, sucrose 10%, inorganic salt 1%, multivitamin 2%, compound amino acid 10%, methyl p-hydroxybenzoate 0.1%, agar 0.2%, distilled water 6.7%. The nutrient composition of this example was used to breed the Aphids aphidis, and the eclosion rate was obtained by an average of 42% per bee card.
[0028] The second group: 25% of tube aphid homogenate, 25% of egg yo...
Embodiment 2
[0035] Embodiment 2: the preparation of artificial aphid
[0036] Melt the agar used for the preparation of the medium with hot water and set aside; add the components of the Aphidius spp. , and add 250-350 units / ml of penicillin; after mixing evenly, use parafilm film as coating material, and make artificial aphids with a diameter of 1.5-2.0mm on a honeycomb machine.
Embodiment 3
[0037] Embodiment 3: the selection of laying eggs attractant component
[0038] In order to improve the egg-laying rate of Aphididae on artificial aphids, the applicant selected the composition and ratio of the egg-laying attractant of A. Compare.
[0039] The first group: Aphids tabaci Nutrient formula: 30% of Aphis persicae homogenate, 20% of egg yolk, 20% of skimmed milk, 10% of sucrose, 1% of inorganic salt, 2% of multivitamins, 10% of compound amino acids, p-hydroxy Methyl benzoate 0.1%, agar 0.2%, distilled water 6.7%.
[0040] After the artificial aphids are prepared by the above formula, the following oviposition attractants are sprayed on the surface of the artificial aphids: 10% sucrose, 10% compound amino acids, 1% nepetalol, 1% nepetalactone, trans-β-farnesene 1%, distilled water 77%.
[0041] Using the nutritional composition of this example and the oviposition attractant to breed the Aphids persicae, the average eclosion rate per bee card was 67%.
[0042] Th...
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