301 results about "Aphis" patented technology

The invention discloses a method for screening and identifying a cucumber aphid-resistant gene as well as a screened gene and application thereof, and provides a brand-new method for screening and identifying the cucumber aphid-resistant gene, which is used for screening the cucumber aphid-resistant gene based on four omics technologies. The four types of omics comprise transcriptome, proteome, methylation group and metabolome before and after inoculation of aphids on cucumbers, and the cucumber aphid resistance regulation gene is screened through combined analysis, so that a new gene resource is provided for improving the aphid resistance of the cucumbers, and a new method and a new idea are provided for designing molecular genetic breeding. The CsPGD2 gene obtained through screening is an aphid resistance gene, the dynamic change of a metabolic substance 5-ribulose phosphate in a metabolome is consistent with that of a transcriptome gene CsPGD2, the aphid resistance function of the gene CsPGD2 is verified through VIGS, and the result proves that the gene CsPGD2 is an important regulation factor for the aphid resistance of cucumbers. The invention provides a new gene resource for improving the aphid resistance of cucumbers, and has a good application prospect.

The invention discloses dsRNA / fused dsRNA for RNAi targeting multiple aphids and application of the dsRNA / fused dsRNA, and the dsRNA targets an aphid Actin gene and / or an ELAV-like protein gene and is obtained by transcription by taking the aphid Actin gene and / or the ELAV-like protein gene as a template. Compared with the traditional design thought that dsRNA of pests mostly aims at one pest and is targeted to a single gene at present, the strategy is changed, starting from the thought that one dsRNA can prevent and treat various aphids, highly conservative genes of various aphids are found, the dsRNA is designed for the highly conservative genes, and it is found that in a plurality of candidate genes, various aphids can be effectively prevented and treated. The dsRNA aiming at the actin Actin gene and the ELAV-like protein gene has a remarkable fatality rate on various aphids and has a very good application prospect, the effect of simultaneously targeting the fused dsRNA of the two genes is further improved, and the dsRNA has a remarkable insecticidal effect on various aphids.

The present disclosure is directed to insect repellant compositions comprising one or more semiochemicals produced by lady beetles. The compositions mimic the pheromones or odor of lady beetles and thus repel aphids and other soft bodied insects that are typically preyed upon by the same. The composition can be used to protect plants, fields, and the like from consumption by these herbivorous insects.

The invention belongs to the technical field of gallnut artificial cultivation, and particularly relates to a method for cultivating gall flowers by using hamburger as a winter host of rhus chinensis gall flower autumn migrant aphids and application, and the method comprises the steps of S1, winter host hamburger planting, S2, gall flower autumn migrant aphid migration, S3, gall flower overwintering aphid collection, and S4, gall flower cultivation. Compared with the prior art, the method has the advantages that the hamburger is used as a winter host of the gall flower aphids, artificial cultivation of the gall flowers is achieved, and the technical problem of how to achieve artificial cultivation of the gall flowers, increase the yield of the gall flowers and further increase the yield of the gallnuts by artificially breeding the gall flower aphids is solved.

The invention discloses an aphid honeydew discharge behavior identification method based on spatio-temporal feature adaptive fusion. The method comprises the following steps: collecting video data of aphids in a natural feeding environment; performing spatio-temporal feature extraction on the original video data by adopting a time sequence weighted frame difference adaptive fusion framework to obtain a motion feature map, inputting the motion feature map into the trained FGC-YOLO small target fine behavior detection model for detection, and outputting a box-selected aphid behavior category result identifier; and carrying out post-processing on an output result of the FGC-YOLO small target fine behavior detection model, and realizing honeydew discharge behavior identification through a dynamic double-cache interpolation mechanism and a composite behavior dynamic judgment algorithm. The method is high in detection precision and good in real-time performance, effectively solves the problems of low aphid honey dew analysis efficiency, poor real-time performance, complex operation and the like through spatial-temporal feature fusion and multi-module cooperation, and is expected to provide technical support for aphid feeding behavior monitoring and pest prevention and control.

The invention belongs to the technical field of molecular marker assisted breeding, and particularly relates to a fresh sweet corn aphid resistance related SNP site combination and application thereof. The SNP (Single Nucleotide Polymorphism) site combination of the aphid-resistant fresh sweet corn comprises an S4174685152 positioned on a chromosome 4, and an S5207733210 and an S5207734430 positioned on a chromosome 5. The developed aphid-resistant functional breeding marker site combination for the fresh-eating sweet corn can assist in breeding of the fresh-eating corn, rapid detection and early detection are achieved, the resistance of the fresh-eating sweet corn to the corn aphid can be predicted only by detecting the genotype of a specific site, and the aphid-resistant functional breeding marker site combination has the advantages of being clear in selection target, easy and convenient to operate and not affected by the environment. The invention provides an effective way for efficient breeding of fresh sweet corn.

The present invention relates to a pyrido[1,2-a]pyrimidine mesoionic derivative containing a 1,2,4-oxadiazole structure and a preparation method and use thereof. The compound has a structure of formula (I), has excellent insecticidal activity against bean aphids, white-backed plant hoppers, diamondback moths, fall armyworms, striped stem borers, armyworms, etc., and can be used to prepare drugs or medicaments for preventing and controlling pests such as aphids, rice plant hoppers, diamondback moths, fall armyworms, striped stem borers, armyworms, etc. The compound has a simple structure and preparation process, and has low production cost.

The artificial breeding and island plant pollination method comprises the following steps: artificial breeding of aphids: broad bean seedlings are planted in an insect breeding cage to propagate black bean aphids, so that adults of the hoverflies can lay fly eggs, and larvae of the hoverflies can eat the fly eggs at the same time; feeding of hoverfly larvae: feeding the hoverfly larvae with black bean aphids in an insect breeding cage, and enabling the hoverfly larvae to develop into pupae after 6 days; collecting pupae of hoverflies with short thorn legs: harvesting broad bean seedlings with mature larvae of hoverflies, putting the broad bean seedlings into a plastic box with a gauze opening for continuous feeding, and putting the collected pupae of hoverflies into a culture dish until all the larvae in the plastic box pupate; and transporting and releasing the short-spur hoverflies, namely storing pupae of the hoverflies in a pupa card device at the temperature of 4 DEG C, transporting the pupae to an open sea island, and releasing the pupae. By the adoption of the method, scale breeding can be conducted on the species of the hoverfly brachypoda, and the hoverfly brachypoda can be transported to the island area and used for pollination of island plants.

The invention relates to the field of image processing, in particular to a small target aphid identification method and device, equipment and a medium. The method comprises the following steps: firstly, acquiring a to-be-processed crop leaf image, and inputting the to-be-processed crop leaf image into a pre-trained small target aphid identification model; according to the model, an image is processed, and a detection frame set of an aphid target and a continuous density map reflecting the spatial aggregation intensity of the aphid target are synchronously output. The training process of the model comprises the steps that farmland scene images are collected, double-label collaborative labeling is carried out, and bounding box labels and continuous density map labels are generated; training a preset model by using the labeled data, wherein the model has multi-scale feature extraction and fusion capabilities; model parameters are optimized through a loss function, an identification model capable of completing target detection and density estimation at the same time is finally obtained, and the detection capacity of small target aphids under the complex background is improved.

The application provides a method for preventing and controlling plant aphids, and the specific steps are as follows: selecting plant germination seeds that have grown roots and the root length is 2-6 cm, applying a light cycle treatment of 8h light and 16h darkness in the growth process of the plant germination seeds; in the plant germination seeds, the seed sources include legumes and cruciferous vegetables; and in the growth process of the plant germination seeds, a boron-containing nutrient solution containing the following components is used: Ca(NO3)2.4H2O 4mM, KH2PO4 1mM, KCl 1mM, MgSO4.7H2O 2mM, K2SO40.5mM, FeSO4.7H2O 50μM, EDTA.Na2 50μM, MnCl2 10μM, ZnSO4.7H2O 5μM, CuSO4.5H2O 0.5μM, (NH4)6Mo7O 24 4H2O 1μM, H3BO3 35-70μM, CoCl20.5μM; the nutrient elements are applied according to the following steps: (1) the first boron-containing nutrient solution is used for 1-5d, and the concentration of all components in the first boron-containing nutrient solution is 20-30% of that of the boron-containing nutrient solution; (2) the second boron-containing nutrient solution is used for 6-15d, and the concentration of all components in the second boron-containing nutrient solution is 40-60% of that of the boron-containing nutrient solution. The method can effectively prevent and control plant aphids.

The invention discloses a three-color composite mulching film and a preparation method thereof. The three-color composite mulching film comprises a transparent bottom layer, a middle black layer, a surface silver layer and a transparent layer located in the middle of the film. The transparent bottom layer has hydrophilicity, and does not form dewdrops on the surface of the film layer to reflect sunlight, so that the light transmittance of the mulching film is effectively increased, and the ground temperature and the soil energy storage are improved. And the middle black layer can efficiently block light rays and can effectively inhibit growth of various weeds. The surface silver layer can highly reflect sunlight, can improve photosynthesis of bottom leaves of crops, and can avoid aphids at the same time.

The invention relates to the technical field of green prevention and control of crops, in particular to an ecological synergistic prevention and control method for diseases and insect pests suitable for strawberry planting in plateau regions, which attracts natural enemy insects by intercropping umbelliferae, compositae and leguminous functional plants, and naturally controls the population number of honeydew type pests such as trialeurodes vaporariorum, aphids and scale insects. The soil fertility is maintained through green manure rotation, the strawberry sooty mould occurrence rate is effectively reduced, the strawberry fruit quality and yield are improved, and the method has the advantages of being green, safe, low in investment and high in benefit.

The application belongs to the technical field of medicine synthesis, and particularly relates to a steroid and 3,4 dihydropyrrole pyrazinone derivative and a synthesis method and application thereof. The derivative has a chemical structure as described in formula (I) or formula (II). The application first proposes a series of novel steroid and 3,4 dihydropyrrole pyrazinone derivatives. In addition, the application also proposes a synthesis method of the steroid and 3,4 dihydropyrrole pyrazinone derivative. The steroid and 3,4 dihydropyrrole pyrazinone derivative is obtained through a series of reactions based on dehydroepiandrosterone. The product prepared by the synthesis method has high yield and is easy to separate, and is the optimal method for preparing the steroid and 3,4 dihydropyrrole pyrazinone derivative. The steroid and 3,4 dihydropyrrole pyrazinone derivative provided by the application is confirmed by biological determination to have good toxic killing activity on aphids, spider mites and oriental armyworm insects, and can be applied to plant pest control.

The invention discloses a method for preventing and controlling aphids in a seedling stage by using predatory mites, which comprises the following steps of: inoculating latheelaelalaelaps wholei serving as the predatory mites on crop seedlings to prevent and control aphid harm. The invention finds and verifies that the latheelaelalaelaps wholei has obvious predation and control effects on the aphids in the seedling stage for the first time, provides a brand-new and efficient biocontrol natural enemy resource for biological control of the aphids, has important ecological and economic values, is suitable for prevention and control of the aphids in the seedling stage of various crops, and has good popularization and application prospects.

The invention provides an Indian kalimeris herb extract for controlling aphids and a preparation method and application thereof, and belongs to the technical field of biological control. The preparation method of the Indian kalimeris herb extract comprises the following steps: mixing Indian kalimeris herb with an organic solvent, performing ultrasonic extraction, filtering, collecting filtrate, and concentrating to obtain the Indian kalimeris herb extract. Potting and field experiments prove that the Indian kalimeris herb extract prepared by the invention has an obvious control effect on cotton aphids and cotton black aphids.

The invention relates to a fluazinam-containing pesticide composition, a seed treatment suspending agent as well as a preparation method and application of the seed treatment suspending agent. The invention provides a fluazinam-containing pesticide composition. The active ingredients of the fluazinam-containing pesticide composition comprise fluazinam, fludioxonil and clothianidin, the mass ratio of the clothianidin to the fluazinam to the fludioxonil is (6-7): (0.4-0.8): (0.2-0.6), and the composition has an excellent synergistic effect. The suspending agent for seed treatment is compounded with a film-forming agent composed of acrylate and polydimethylsiloxane according to a specific proportion, it can be ensured that seed coats are not prone to falling off after being coated, meanwhile, adjustment can be conducted according to environmental adaptability, a coating layer is evenly mixed with a pesticide and pigment, the release time of effective components of pesticides is prolonged, and the pesticide effect is improved. The problem of seed coating fading is effectively solved, and the pesticide composition has an excellent effect in prevention and treatment of agricultural diseases such as peanut root rot, grub, wheat stem rot and aphid.

The present invention relates to insect neuropeptide compounds of the family of pyroglutamic peptides and analogs thereof having activity against insects, such as Hemiptera, Diptera, Lepidoptera, Blattaria and / or Coleoptera insects, such as aphids and fruit flies, and their use as insect control agents (e.g. insecticides) and plant protection agents.

The invention belongs to the field of microbial technology, relates to the screening, identification and application of functional bacteria, and discloses a black Aspergillus and its fermentation method and application. The black Aspergillus provided by the invention is Aspergillus niger YXlfs22.3, and its deposit number is CGMCC No.40261. The black Aspergillus Aspergillus niger YXlfs22.3 bacterial body and / or fermentation liquid provided by the invention can effectively prevent and control aphids: after the fermentation liquid stock solution is applied to the leaves of melon plants for 5 days, the insect population reduction rate is 96.19%, and the corrected control effect is 95.88%; after the fermentation liquid diluted 10 times is applied to the leaves of watermelon plants for 48 hours, the insect population reduction rate is 90.01%, and the corrected control effect is 86.89%. The invention can replace traditional pesticides and be used for preventing and controlling plant aphid pests, and has the advantages of low preparation cost, no pollution to the environment, high cost performance, etc.

The invention relates to a pesticide composition containing ecdysone compounds and application of the pesticide composition. The pesticide composition is composed of flufeniprole and methoxyfenozide or tebufenozide. The pesticide composition has an obvious synergistic effect, and the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The pesticide composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, tryporyza incertulas, rice leaf folders, plant hoppers, prodenia litura, beet armyworms, plutella xylostella, cabbage caterpillars, aphids, aleyrodids, thrips and flea beetles.

The invention discloses a botanical insecticide and a preparation method thereof, and relates to the technical field of botanical insecticide preparation, and the botanical insecticide comprises the following components: potassium carbonate, and 30 g / 15 kg of water; the dosage of the glucoside is 50-60g / 15kg of water; optionally, 0.1%-0.5% of a plant source synergist is added; the plant source synergist is one of sophocarpidine or azadirachtin; dissolving potassium carbonate in 15kg of water, and stirring until the potassium carbonate is completely dissolved; adding glucoside, and continuously stirring until the materials are uniformly mixed; adding a botanical synergist at the final stage, and fully and uniformly mixing; according to the insecticidal composition, potassium carbonate, glucoside and a plant source synergist are selected as main components, effective prevention and control of pests such as aphids, red spiders and trialeurodes vaporariorum are achieved, use of chemical pesticides is reduced through natural components, accordingly, negative effects on the environment and non-target organisms are reduced, the environment-friendly and efficient insecticidal effect is achieved, and the insecticidal composition is worthy of popularization and application. Dissolving potassium carbonate in water, stirring until the potassium carbonate is completely dissolved, then adding glucoside, finally adding the plant-source synergist, and fully and uniformly mixing.

The invention belongs to the technical field of cosmetics, and discloses an avocado fermentation composition as well as a preparation method and application thereof. The composition comprises 1%-10% of avocado fermentation filtrate, 0.1%-2% of alpha-glucan oligosaccharide, 0.1%-2% of a wild chrysanthemum flower extract, 3%-8% of a humectant, 0.01%-0.1% of a chelating agent and the balance of deionized water. Wherein the avocado fermentation filtrate is prepared by compound fermentation of aphid torulopsis and candida and is rich in oligopeptide, free amino acid, lactic acid and polyphenol. According to the invention, the filtrate obtained by fermenting avocado with a specific composite strain is scientifically compounded with prebiotics with definite flora selectivity to construct a multifunctional composition with definite structure, synergistic function and high stability; the composition not only provides direct barrier repairing and moisturizing effects, but also actively guides skin micro-ecology to succession to a healthy steady state through nutrition intervention, so that the skin health level is synchronously improved in the two dimensions of molecules and ecology.

The invention relates to application of limonin as a pesticide synergistic aid in prevention and control of corn pests, which utilizes the characteristic that low-concentration limonin has a remarkable synergistic effect on chemical pesticides, 0.5-4g / mL limonin, 5% chlorantraniliprole and 25% thiamethoxam are mixed for use, so that the lethal medium concentration (LC50) of the limonin to corn borers, corn aphids and rhopalosiphum padi can be remarkably reduced; the insecticidal effect of chlorantraniliprole on corn borer and the insecticidal effect of thiamethoxam on aphid are improved. A field pesticide effect test also shows that the field prevention and control effect of the pesticide on Lepidoptera pests such as ostrinia nubilalis is remarkably improved by mixing 4 g / mL of limonin and 5% of chlorantraniliprole, the actual field dosage of chemical pesticides is reduced, the purposes of pesticide reduction and synergism are achieved, and a positive effect is achieved on comprehensive prevention and control of the field pests.

The invention discloses application of a corn RTA9 gene in enhancing aphid resistance of plants in the technical field of plant genetic engineering, a CDS sequence of the corn RTA9 gene is shown as SEQ ID NO.1, an amino acid sequence for coding the corn RTA9 gene is shown as SEQ ID NO.2, subcellular localization shows that RTA9 is mainly localized in mitochondria, and it is known through research of the invention that the corn RTA9 gene can be used for improving aphid resistance of plants. The function deletion of the RTA9 gene weakens the capability of producing ROS (reactive oxygen species) in response to aphid infection of the corn, so that the aphid resistance of the corn is reduced. The content of ROS (reactive oxygen species) in cells can be remarkably increased by overexpressing RTA9 in corn, meanwhile, the aphid resistance of corn is improved, and a theoretical basis and gene resources are provided for developing aphid-resistant crop strains.

The invention relates to a method for analyzing effective components of a flonicamid-veratrine compound preparation. The effective components flonicamid and veratrine in the compound preparation are quantitatively analyzed at the same time by a high performance liquid chromatography external standard method. The binary compound insecticide taking the flonicamid and the veratrine as the effective components has an obvious synergistic effect, is good in fast-acting property and persistent effect and relatively small in dosage of the effective components, reduces the production cost, and is suitable for preventing and treating aphids on cucumber crops.

The invention discloses a composition for repelling aphids and preventing potato Y virus infection and a virus and insect inhibitor, and belongs to the technical field of plant disease prevention and control. The pharmaceutical composition provided by the invention is prepared from at least two of 1-pentene-3-ketone, 2-ethyl furan and cis-4-heptenal, and the pharmaceutical composition is prepared from at least two of 1-pentene-3-ketone, 2-ethyl furan and cis-4-heptenal. The pharmaceutical composition not only can avoid aphids and reduce the probability that crops are infected by potato Y viruses, but also can realize the synergistic interaction among the drugs and avoid or reduce the drug resistance of the aphids to the drugs, so that the pharmaceutical composition has important practical value and application prospect in the technical field of plant disease control.

The present invention relates to a mixture of volatile organic compounds (VOCs) for insect repellency, in particular for aphids, which comprises as VOCs at least linalool and eugenol. The invention also relates to an insect repellent composition comprising said mixture, and its use in agriculture, in particular in fields for protecting plants against damage caused by insect pests.

The utility model discloses an aphid evading device in the technical field of aphid evading, which comprises a stainless steel barrel, the top of the stainless steel barrel is fixedly connected with a barrel cover through bolts, the middle of the bottom of the stainless steel barrel is fixedly connected with an electric heating plate, the middle of the top of the barrel cover is fixedly connected with a mounting pipe, and the mounting pipe is fixedly connected with an electric heating plate. A mounting pipe is fixedly connected to the top of the barrel cover, a high-temperature-resistant plastic fan is fixedly connected to the inner cavity wall of the mounting pipe, a U-shaped mounting frame is fixedly connected to the top of the barrel cover, an L-shaped pipe is rotatably connected to the middle of the top of the U-shaped mounting frame, and the top end of the mounting pipe is inserted into an inner cavity of the L-shaped pipe. According to the liquid level detection device for the repellent, the volatilization rate of the repellent in use can be increased, the distribution range of the repellent is expanded, meanwhile, distribution is more uniform, and therefore the repellent effect is improved, the liquid level height of the repellent can be detected in the use process, and the safety in the use process is guaranteed while the continuity of the repellent effect is guaranteed.

The invention relates to the technical field of agricultural equipment, and discloses an agricultural leaf vegetable insect suction plant protection device which comprises a moving device, a pesticide module is arranged on the moving device, a pesticide spraying module communicated with the pesticide module is installed on the moving device, and the pesticide spraying module is provided with a plurality of adjustable output ends. According to the device, a collaborative operation system of a low-temperature atomization branch, a pesticide spraying pipe and the pest sucking module is innovatively constructed, the pest catching efficiency is enhanced from multiple links, the low-temperature atomization branch cools, atomizes and sprays out a pesticide, the pesticide spraying pipe sprays out the pesticide, the pesticide spraying pipe sprays out the pesticide, the pesticide spraying pipe sprays out the pesticide, and the pest sucking module sprays out the pesticide. Pests such as aphids and cabbage caterpillars are enabled to quickly enter a'cold paralysis' state by utilizing low temperature, nerve conduction and muscle contraction of the pests are inhibited due to low temperature, the action ability is obviously slowed down, and the possibility of active escape is reduced; and the killing is realized by more sufficient attachment and permeation.

The invention provides a high-precision aphid identification and counting method in a mobile computing scene, and relates to the technical field of agricultural pest intelligent monitoring, and the method comprises the steps: taking a monitored plant as a center, collecting a plurality of groups of visible light images and depth images along an annular path, and constructing a plant three-dimensional point cloud model through coordinate registration and point cloud fusion; segmenting and extracting independent blade units by adopting a self-adaptive region growing algorithm, and carrying out plane fitting and two-dimensional projection to generate a blade emmetronized image; recognizing the envisaged image through a pre-trained aphid recognition model to obtain an aphid bounding box set and a fine-grained category; establishing a mapping relationship between the three-dimensional point cloud and the two-dimensional image, and mapping the bounding box back to the three-dimensional point cloud to obtain an aphid candidate point set; and carrying out double-constrained three-dimensional clustering by utilizing spatial Euclidean distance and fine-grained category label consistency, and screening and marking independent aphid individuals. The method can realize high-precision aphid identification and counting, and can be widely applied to the field of agricultural pest intelligent monitoring.