375 results about "Aphis" patented technology

The utility model relates to the field of agricultural disease and pest control, in particular to an agricultural disease and pest control device which comprises a device base, a pesticide box and a driving box, the pesticide box is fixedly connected to the middle of the top of the device base, and the driving box is fixedly connected to the middle of the top of the pesticide box. The agricultural pest control device comprises a pesticide box, a driving box is arranged in the pesticide box, a motor is fixedly connected to the inner top of the driving box, a second rotating shaft is fixedly connected to the driving position of the motor, and second stirring blades are fixedly connected to the periphery of the lower portion of the second rotating shaft. Meanwhile, by installing a water pipe, a water pump and an atomization spray head, part of pesticide liquid is sprayed to the back faces of crop leaves through a bottom auxiliary atomization spray head, aphids and other pests are prevented from being attached to the back faces of the leaves, and the control effect is limited.

The invention discloses an isoxazoline compound as shown in a formula (I) and application thereof. R1, R2 and R3 can be selected from halogen atoms, cyano groups, nitryl groups, C1-C3 halogenated alkyl groups and the like; q can be independently selected from A1 to A6; l1 and L2 may be selected from a carboxyl group, a C1-C6 alkoxycarbonyl group, a hydroxyl-substituted C1-C8 alkyl group, and the like; z1, Z2 may be selected from a hydrogen atom, a C1-C6 alkyl group, a C3-C5 cycloalkyl group, or C (O) M, wherein M is a C1-C6 alkyl group, a C2-C6 alkenyl group, or the like; the isoxazoline compound provided by the invention has no cross resistance with existing insecticides, has the advantages of quick pesticide effect, low dosage and the like, and particularly has an excellent control effect on rice-resistant chilo suppressalis, plutella xylostella, aphid and the like. The invention also provides an isoxazoline compound as a pesticide acceptable salt, a composition and an application of the isoxazoline compound. # imgabs0 #

The invention relates to the technical field of biology, and particularly provides preparation and application of a dsMpC002-coated ZIF-8 nano complex. According to the invention, a green peach aphid effector dsMpC002 is taken as a target, a ZIF-8 delivery system of the green peach aphid effector dsMpC002 with a stable superexcellent target is constructed, and a dsMpC002-ZIF-8 nano-composite is prepared and obtained. By observing and analyzing the MpC002 gene expression quantity, the death rate and the single female farrowing quantity of the green peach aphid after the pepper variety DYJ sprayed with the dsMpC002-coated ZIF-8 nano-complex with different concentrations is fed, the control effect of the dsMpC002-coated ZIF-8 nano-complex on the green peach aphid is determined. And a new target technology and a new idea are provided for innovating an aphid control method.

The invention discloses a method for screening and identifying a cucumber aphid-resistant gene as well as a screened gene and application thereof, and provides a brand-new method for screening and identifying the cucumber aphid-resistant gene, which is used for screening the cucumber aphid-resistant gene based on four omics technologies. The four types of omics comprise transcriptome, proteome, methylation group and metabolome before and after inoculation of aphids on cucumbers, and the cucumber aphid resistance regulation gene is screened through combined analysis, so that a new gene resource is provided for improving the aphid resistance of the cucumbers, and a new method and a new idea are provided for designing molecular genetic breeding. The CsPGD2 gene obtained through screening is an aphid resistance gene, the dynamic change of a metabolic substance 5-ribulose phosphate in a metabolome is consistent with that of a transcriptome gene CsPGD2, the aphid resistance function of the gene CsPGD2 is verified through VIGS, and the result proves that the gene CsPGD2 is an important regulation factor for the aphid resistance of cucumbers. The invention provides a new gene resource for improving the aphid resistance of cucumbers, and has a good application prospect.

The invention discloses a sorghum aphid MsCYP1 gene as well as an encoding protein, dsRNA (double-stranded ribonucleic acid) and application thereof, and aims to solve the technical problem that a key gene is lacked when an RNA (ribonucleic acid) interference technology is used for biological prevention and control of sorghum aphids at present. According to the application, the sorghum aphid protein MsCYP1 capable of being secreted into sorghum cells is screened, and the protein and the coding gene thereof can inhibit the content of hydrogen peroxide in sorghum and the immune response of the sorghum. Meanwhile, according to the cDNA sequence of the MsCYP1 gene of the sorghum aphid, dsRNA for the gene is designed, the dsRNA is introduced into the body of the sorghum aphid in an artificial feeding mode, it is found that the dsRNA can remarkably inhibit the MsCYP1 gene of the sorghum aphid, then the honeydew secretion amount and the reproduction rate of the sorghum aphid are affected, the MsCYP1 gene can serve as an RNAi target gene to be used for aphid prevention and control, pesticide use is reduced, and the application prospect is good. The ecological balance is maintained.

The present invention relates to natural or natural-like analogs of insect pyrokinin neuropeptide [Hy]-NADEDQQQSVDFTPRL-[NH2] (SEQ ID NO: 2) having activity against insects, such as hemiptera, diptera, lepidoptera, cockroaches and / or coleopteran insects, such as aphids, moths and fruit flies, and the use of these natural or natural-like analogs as insect control agents, such as insecticides, and plant protection agents.

The insecticidal microemulsion composition compounded by flonicamid and spinetoram provided by the invention can be used for preventing and treating various pests such as aphids, leafhoppers, plant hoppers, thrips and the like, and has a special effect on preventing and treating eggplant thrips. According to the composition disclosed by the invention, the polyether modified organic silicon is added as a synergist, so that the pesticide effect of the flonicamid and spinetoram compound microemulsion can be obviously improved. The polyether modified organic silicon can also reduce the lasting foamability of the pesticide, so that the effect of the pesticide is prevented from being reduced due to foamability of the pesticide liquid.

The invention relates to the technical field of agriculture, and discloses a biological organic bacterial fertilizer capable of improving plant disease resistance, which comprises the following components in percentage by mass: 30-50% of bacillus liquid; 30%-50% of a trichoderma bacteria solution; 5%-10% of a chitosan solution; 5%-15% of a sodium alginate solution; 2%-5% of a composite solution; 1% to 5% of a nanocomposite; the composite solution comprises the following components in percentage by mass: 15%-18% of artemisia capillaris; 7%-12% of tripterygium wilfordii; 9%-17% of radix astragali; 15%-19% of fructus cnidii; and 12%-17% of raw licorice. Through effective combination of traditional Chinese medicine components and beneficial bacteria, the insect pest resistance and disease resistance of crops are remarkably enhanced, breeding of aphids, red spiders and other pests can be effectively inhibited, fungal diseases such as downy mildew, powdery mildew and the like are prevented, the immunoreaction of plants is enhanced, and the disease incidence rate is fundamentally reduced.

The present invention relates to natural or natural-like analogs of insect pyrokinin neuropeptide [Hy]-GGSTFSPRL-[NH2] (SEQ ID NO: 2) having activity against insects, such as hemiptera, diptera, lepidoptera, cockroaches and / or coleopteran insects, such as aphids, moths and fruit flies, and the use of these natural or natural-like analogs as insect control agents, such as insecticides, and plant protection agents.

The invention discloses dsRNA / fused dsRNA for RNAi targeting multiple aphids and application of the dsRNA / fused dsRNA, and the dsRNA targets an aphid Actin gene and / or an ELAV-like protein gene and is obtained by transcription by taking the aphid Actin gene and / or the ELAV-like protein gene as a template. Compared with the traditional design thought that dsRNA of pests mostly aims at one pest and is targeted to a single gene at present, the strategy is changed, starting from the thought that one dsRNA can prevent and treat various aphids, highly conservative genes of various aphids are found, the dsRNA is designed for the highly conservative genes, and it is found that in a plurality of candidate genes, various aphids can be effectively prevented and treated. The dsRNA aiming at the actin Actin gene and the ELAV-like protein gene has a remarkable fatality rate on various aphids and has a very good application prospect, the effect of simultaneously targeting the fused dsRNA of the two genes is further improved, and the dsRNA has a remarkable insecticidal effect on various aphids.

The present disclosure is directed to insect repellant compositions comprising one or more semiochemicals produced by lady beetles. The compositions mimic the pheromones or odor of lady beetles and thus repel aphids and other soft bodied insects that are typically preyed upon by the same. The composition can be used to protect plants, fields, and the like from consumption by these herbivorous insects.

The present invention relates to a Cucurbitaceae plant that comprises a CABYV resistance locus providing resistance to Cucurbit aphid-borne yellows disease and / or the Cucurbitaceae plant wherein said plant comprises a stay green (SGR) gene providing improved fruit shelf life and reduced leaf yellowing. The present invention further relates to a CABYV resistance gene and / or an SGR gene, the use of said gene(s) for providing a Cucurbitaceae plant resistant to Cucurbit aphid-borne yellows disease and methods for identifying or selecting a Cucurbitaceae plant resistant to Cucurbit aphid-borne yellows disease and / or leaf yellowing.

The present invention provides a Bacillus orientalis with the function of preventing and controlling cabbage aphids, and the deposit number thereof is CGMCC No. 28561. The Bacillus orientalis HXBW-2 strain screened by the present invention can alleviate plant pests caused by cabbage aphids, has good insecticidal effects both indoors and outdoors, and has development potential.

The present invention relates to a mutant plasmid and a preparation method and application thereof, belonging to the field of plant virology and molecular biology. F R2‑2bPT‑PSTVd 1‑200 Contains CMV Fny The full-length RNA2 sequence of the isolate has TAATAG and PSTVd ​​fragment inserted between positions 2682 and 2683; the nucleotide sequence of the PSTVd ​​fragment is shown in SEQ ID NO.1. Fny Isolate RNA1 and modified PCB301‑RNA3‑ △ The apno plasmid and the mutant plasmid of the present invention are pre-mixed and inoculated. The attenuated vaccine can prevent the potato spindle tuber virus from causing harm and avoid the spread of the virus by aphids in the field. The effect is good and provides vaccine materials and effective measures for field control of PSTVd.

The invention belongs to the technical field of gallnut artificial cultivation, and particularly relates to a method for cultivating gall flowers by using hamburger as a winter host of rhus chinensis gall flower autumn migrant aphids and application, and the method comprises the steps of S1, winter host hamburger planting, S2, gall flower autumn migrant aphid migration, S3, gall flower overwintering aphid collection, and S4, gall flower cultivation. Compared with the prior art, the method has the advantages that the hamburger is used as a winter host of the gall flower aphids, artificial cultivation of the gall flowers is achieved, and the technical problem of how to achieve artificial cultivation of the gall flowers, increase the yield of the gall flowers and further increase the yield of the gallnuts by artificially breeding the gall flower aphids is solved.

The invention belongs to the technical field of microbial fertilizers, and particularly relates to a disease and pest resistant microbial fertilizer based on traditional Chinese medicinal materials and a preparation method. The pest and disease resistant microbial fertilizer is prepared from the following raw materials in parts by mass: 15-25 parts of a traditional Chinese medicinal material extract, 70-100 parts of a composite carrier, 2-4 parts of composite microbial flora, 5-10 parts of potassium fulvate, 5-10 parts of sodium humate, 5-10 parts of polyglutamic acid and 15-25 parts of water, the pest and disease resistant microbial fertilizer provided by the invention not only has high viable count, but also has good stability, and can inhibit powdery mildew, downy mildew and aphids of cucumbers at the same time, so that the cucumbers have high acre yield, soluble total sugar content and soluble solid content.

The utility model discloses an aphid cultivation and observation cage, which comprises a cage body, the cage body comprises two half cages, the two half cages are provided with concave spaces, the two half cages are folded to form a cultivation space in which leaves are covered, the aphid cultivation and observation cage further comprises an elastic hinge, the elastic hinge is provided with two function ends with closing tendency, and the function ends are connected with the elastic hinge. The two functional ends are connected to the corresponding half cages respectively, so that the two half cages are in a closed state when not subjected to external force, an inserting sleeve is further arranged on the edge of one of the half cages, and a supporting rod is detachably inserted into the inserting sleeve. According to the scheme, through the structure, covering of aphid cultivation leaves is achieved, cultivation of aphids close to the natural environment is achieved, and data closest to the natural environment is provided for prevention and control of the aphids.

The invention discloses an aphid honeydew discharge behavior identification method based on spatio-temporal feature adaptive fusion. The method comprises the following steps: collecting video data of aphids in a natural feeding environment; performing spatio-temporal feature extraction on the original video data by adopting a time sequence weighted frame difference adaptive fusion framework to obtain a motion feature map, inputting the motion feature map into the trained FGC-YOLO small target fine behavior detection model for detection, and outputting a box-selected aphid behavior category result identifier; and carrying out post-processing on an output result of the FGC-YOLO small target fine behavior detection model, and realizing honeydew discharge behavior identification through a dynamic double-cache interpolation mechanism and a composite behavior dynamic judgment algorithm. The method is high in detection precision and good in real-time performance, effectively solves the problems of low aphid honey dew analysis efficiency, poor real-time performance, complex operation and the like through spatial-temporal feature fusion and multi-module cooperation, and is expected to provide technical support for aphid feeding behavior monitoring and pest prevention and control.

The invention belongs to the technical field of molecular marker assisted breeding, and particularly relates to a fresh sweet corn aphid resistance related SNP site combination and application thereof. The SNP (Single Nucleotide Polymorphism) site combination of the aphid-resistant fresh sweet corn comprises an S4174685152 positioned on a chromosome 4, and an S5207733210 and an S5207734430 positioned on a chromosome 5. The developed aphid-resistant functional breeding marker site combination for the fresh-eating sweet corn can assist in breeding of the fresh-eating corn, rapid detection and early detection are achieved, the resistance of the fresh-eating sweet corn to the corn aphid can be predicted only by detecting the genotype of a specific site, and the aphid-resistant functional breeding marker site combination has the advantages of being clear in selection target, easy and convenient to operate and not affected by the environment. The invention provides an effective way for efficient breeding of fresh sweet corn.

The present invention relates to a pyrido[1,2-a]pyrimidine mesoionic derivative containing a 1,2,4-oxadiazole structure and a preparation method and use thereof. The compound has a structure of formula (I), has excellent insecticidal activity against bean aphids, white-backed plant hoppers, diamondback moths, fall armyworms, striped stem borers, armyworms, etc., and can be used to prepare drugs or medicaments for preventing and controlling pests such as aphids, rice plant hoppers, diamondback moths, fall armyworms, striped stem borers, armyworms, etc. The compound has a simple structure and preparation process, and has low production cost.

The artificial breeding and island plant pollination method comprises the following steps: artificial breeding of aphids: broad bean seedlings are planted in an insect breeding cage to propagate black bean aphids, so that adults of the hoverflies can lay fly eggs, and larvae of the hoverflies can eat the fly eggs at the same time; feeding of hoverfly larvae: feeding the hoverfly larvae with black bean aphids in an insect breeding cage, and enabling the hoverfly larvae to develop into pupae after 6 days; collecting pupae of hoverflies with short thorn legs: harvesting broad bean seedlings with mature larvae of hoverflies, putting the broad bean seedlings into a plastic box with a gauze opening for continuous feeding, and putting the collected pupae of hoverflies into a culture dish until all the larvae in the plastic box pupate; and transporting and releasing the short-spur hoverflies, namely storing pupae of the hoverflies in a pupa card device at the temperature of 4 DEG C, transporting the pupae to an open sea island, and releasing the pupae. By the adoption of the method, scale breeding can be conducted on the species of the hoverfly brachypoda, and the hoverfly brachypoda can be transported to the island area and used for pollination of island plants.

The present invention relates to natural or natural-like analogues of insect pyrokinin neuropeptide [Pyr]-AIMARPQVPRL-[NH2] (SEQ ID NO: 2) having activity against insects, for example hemipteran, dipteran, lepidopteran, blattodean and / or coleopteran insects, such as aphids, moths and fruit flies, and their use as insect control agents (e.g. insecticides) and plant protection agents.

The invention relates to the field of image processing, in particular to a small target aphid identification method and device, equipment and a medium. The method comprises the following steps: firstly, acquiring a to-be-processed crop leaf image, and inputting the to-be-processed crop leaf image into a pre-trained small target aphid identification model; according to the model, an image is processed, and a detection frame set of an aphid target and a continuous density map reflecting the spatial aggregation intensity of the aphid target are synchronously output. The training process of the model comprises the steps that farmland scene images are collected, double-label collaborative labeling is carried out, and bounding box labels and continuous density map labels are generated; training a preset model by using the labeled data, wherein the model has multi-scale feature extraction and fusion capabilities; model parameters are optimized through a loss function, an identification model capable of completing target detection and density estimation at the same time is finally obtained, and the detection capacity of small target aphids under the complex background is improved.

The application provides a method for preventing and controlling plant aphids, and the specific steps are as follows: selecting plant germination seeds that have grown roots and the root length is 2-6 cm, applying a light cycle treatment of 8h light and 16h darkness in the growth process of the plant germination seeds; in the plant germination seeds, the seed sources include legumes and cruciferous vegetables; and in the growth process of the plant germination seeds, a boron-containing nutrient solution containing the following components is used: Ca(NO3)2.4H2O 4mM, KH2PO4 1mM, KCl 1mM, MgSO4.7H2O 2mM, K2SO40.5mM, FeSO4.7H2O 50μM, EDTA.Na2 50μM, MnCl2 10μM, ZnSO4.7H2O 5μM, CuSO4.5H2O 0.5μM, (NH4)6Mo7O 24 4H2O 1μM, H3BO3 35-70μM, CoCl20.5μM; the nutrient elements are applied according to the following steps: (1) the first boron-containing nutrient solution is used for 1-5d, and the concentration of all components in the first boron-containing nutrient solution is 20-30% of that of the boron-containing nutrient solution; (2) the second boron-containing nutrient solution is used for 6-15d, and the concentration of all components in the second boron-containing nutrient solution is 40-60% of that of the boron-containing nutrient solution. The method can effectively prevent and control plant aphids.

The invention discloses a three-color composite mulching film and a preparation method thereof. The three-color composite mulching film comprises a transparent bottom layer, a middle black layer, a surface silver layer and a transparent layer located in the middle of the film. The transparent bottom layer has hydrophilicity, and does not form dewdrops on the surface of the film layer to reflect sunlight, so that the light transmittance of the mulching film is effectively increased, and the ground temperature and the soil energy storage are improved. And the middle black layer can efficiently block light rays and can effectively inhibit growth of various weeds. The surface silver layer can highly reflect sunlight, can improve photosynthesis of bottom leaves of crops, and can avoid aphids at the same time.

The invention relates to the technical field of green prevention and control of crops, in particular to an ecological synergistic prevention and control method for diseases and insect pests suitable for strawberry planting in plateau regions, which attracts natural enemy insects by intercropping umbelliferae, compositae and leguminous functional plants, and naturally controls the population number of honeydew type pests such as trialeurodes vaporariorum, aphids and scale insects. The soil fertility is maintained through green manure rotation, the strawberry sooty mould occurrence rate is effectively reduced, the strawberry fruit quality and yield are improved, and the method has the advantages of being green, safe, low in investment and high in benefit.

The present invention relates to a lettuce (Lactuca sativa) plant, which has the resistance against Nasonovia ribisnigri biotype 1 (Nr:1) as found in plants grown from seeds of Lactuca serriola 10G.913571 representative seeds of which were deposited under NCIMB accession number 41776. Such a lettuce plant is obtainable by introgressing the resistance to Nasonovia risbisnigri Nr:1 as found in Lactuca serriola 10G.913571 representative seeds of which were deposited under NCIMB accession number 41776 into a plant that is not resistant.

The utility model discloses a gallnut aphid bag sealing device, which belongs to the technical field of gallnut production, and comprises a sealing bag, the sealing bag is of a right triangle structure, the sealing bag is made of kraft paper, the bottom of the inner side of the sealing bag is provided with an opening object, the opening object is in the size of a little finger, and the opening object is provided with an opening hole. And the opening object is made of a soft smoke-free paper material. The Chinese gall aphid bag sealing device is made of smokeless paper which is free of mildew, good in air permeability, high in aphid survival rate, easy in material obtaining and low in cost, and solves the problems that existing Chinese gall aphid bag sealing devices mostly adopt corncobs, toilet paper, paper balls or cotton as opening objects, large humidity is needed in a cultivation room, cultivation time is long, and cost is high. Corncobs, toilet paper and paper balls are easy to damp and mildew when being used as strutting objects, and the survival rate of aphids is low.

The application belongs to the technical field of medicine synthesis, and particularly relates to a steroid and 3,4 dihydropyrrole pyrazinone derivative and a synthesis method and application thereof. The derivative has a chemical structure as described in formula (I) or formula (II). The application first proposes a series of novel steroid and 3,4 dihydropyrrole pyrazinone derivatives. In addition, the application also proposes a synthesis method of the steroid and 3,4 dihydropyrrole pyrazinone derivative. The steroid and 3,4 dihydropyrrole pyrazinone derivative is obtained through a series of reactions based on dehydroepiandrosterone. The product prepared by the synthesis method has high yield and is easy to separate, and is the optimal method for preparing the steroid and 3,4 dihydropyrrole pyrazinone derivative. The steroid and 3,4 dihydropyrrole pyrazinone derivative provided by the application is confirmed by biological determination to have good toxic killing activity on aphids, spider mites and oriental armyworm insects, and can be applied to plant pest control.