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56 results about "Leafhopper" patented technology

A leafhopper is the common name for any species from the family Cicadellidae. These minute insects, colloquially known as hoppers, are plant feeders that suck plant sap from grass, shrubs, or trees. Their hind legs are modified for jumping, and are covered with hairs that facilitate the spreading of a secretion over their bodies that acts as a water repellent and carrier of pheromones. They undergo a partial metamorphosis, and have various host associations, varying from very generalized to very specific. Some species have a cosmopolitan distribution, or occur throughout the temperate and tropical regions. Some are pests or vectors of plant viruses and phytoplasmas. The family is distributed all over the world, and constitutes the second-largest hemipteran family, with at least 20,000 described species.

Insecticidal microemulsion composition

The insecticidal microemulsion composition compounded by flonicamid and spinetoram provided by the invention can be used for preventing and treating various pests such as aphids, leafhoppers, plant hoppers, thrips and the like, and has a special effect on preventing and treating eggplant thrips. According to the composition disclosed by the invention, the polyether modified organic silicon is added as a synergist, so that the pesticide effect of the flonicamid and spinetoram compound microemulsion can be obviously improved. The polyether modified organic silicon can also reduce the lasting foamability of the pesticide, so that the effect of the pesticide is prevented from being reduced due to foamability of the pesticide liquid.
Owner:SHANGHAI YUELIAN BIOLOGICAL TECH +1

Odorant binding protein from black-tail leafhopper and application of odorant binding protein in prevention and treatment of rice dwarf virus

The invention provides an odorant binding protein sourced from cicada and application of the odorant binding protein in prevention and treatment of rice dwarf disease. The amino acid sequence of the odorant binding protein is SEQ ID NO: 1. According to the application disclosed by the invention, a fluorescent competitive binding experiment finds that the odorant binding protein NcinOBP8 of the black tail leafhopper and a volatile matter released by rice induced by a rice dwarf virus have high affinity; the RNAi experiment shows that the rice volatile matter induced by the rice dwarf virus has an attraction effect on the black-tail leafhopper nymphs injected with dsEGFP, but after the NcinOBP8 is silenced, the rice volatile matter does not attract the black-tail leafhopper nymphs after 2 hours. Therefore, the silent cicada NcinOBP8 can influence the perception of the cicada on the rice volatile matters.
Owner:QINGDAO AGRI UNIV

A composition for preventing and treating cotton leafhoppers, an insecticide and application thereof and a preparation method thereof

The present application relates to the technical field of cotton leafhopper control, and particularly relates to a composition for preventing and treating cotton leafhopper, an insecticide and application thereof and a preparation method thereof.The composition comprises dinotefuran, potassium dihydrogen phosphate, orange peel essential oil and eucalyptus oil, wherein the mass fraction of dinotefuran is 67.5-94.5 parts, the mass fraction of potassium dihydrogen phosphate is 900 parts, the mass fraction of orange peel essential oil is 900 parts, and the mass fraction of eucalyptus oil is 7.5 parts.The insecticide in the present application can not only achieve better control effect, but also reduce the dosage of dinotefuran when applied to the control of cotton leafhopper, so as to effectively delay the development of drug resistance of cotton leafhopper.
Owner:INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI

Application of RdCDC3 gene in precise prevention and control of rice pest leafhopper

The invention discloses an application of an RdCDC3 gene in precise prevention and control of a rice pest leafhopper. The invention provides a substance for reducing the content or activity of RdCDC3 protein or inhibiting the expression of a coding gene of the RdCDC3 protein. The substance is applied to any one of the following applications: A1) inducing natural enemies of leafhoppers to eat; a2) preventing and controlling leafhoppers; a3) preventing and controlling plant diseases caused or mediated by leafhoppers; a4) preparing a product for inducing the natural enemy of leafhopper to eat; a5) preparing a product for preventing and controlling leafhoppers; a6) preparing a product for preventing and controlling leafhopper-caused or mediated plant diseases; according to the present invention, with the artificially prepared RdCDC3 targeting siRNA, the RdCDC3 in the leafhopper egg can be effectively silenced, such that the accurate targeting can be performed on the RdCDC3 target spot; by means of the method, the leafhopper can be effectively controlled on the premise that ecological balance is not damaged, and a new technical scheme is provided for green agricultural prevention and control.
Owner:PEKING UNIV

Application of geraniol in inhibition of egg laying and egg incubation of tea lesser leafhopper

The invention provides application of geraniol in inhibition of egg laying and egg incubation of tea lesser leafhopper, and belongs to the technical field of pest control. Empoasca flavescens oviposition and egg incubation are inhibited by fumigating geraniol. The influence of geraniol with different concentrations on the egg laying amount and egg hatching rate of the tea lesser leafhoppers is researched, and the result shows that egg laying and egg hatching of the tea lesser leafhoppers can be remarkably inhibited by adopting geraniol with the concentration of 1%, 5% and 10% for fumigation treatment. The geraniol can effectively reduce the population growth rate of the tea lesser leafhopper, is expected to be prepared into a natural preparation for preventing and treating the tea lesser leafhopper, and has the advantages of safety, high efficiency and sustainability.
Owner:HAINAN RES INST OF ZHEJIANG UNIV

40% diafenthiuron-flonicamid suspending agent and preparation method thereof

The invention relates to the field of chemical pesticides, in particular to a 40% diafenthiuron-flonicamid suspending agent and a preparation method thereof. The 40% diafenthiuron-flonicamid suspending agent provided by the invention is prepared from the following raw materials in parts by mass: 30 to 40 parts of diafenthiuron active compound, 5 to 10 parts of flonicamid active compound, 5 to 10 parts of dispersing agent, 1 to 10 parts of anti-freezing agent, 0.1 to 10 parts of thickening agent, 0.1 to 1 part of functional additive and 30 to 50 parts of deionized water. Wherein the dispersing agent comprises a comb-shaped high-molecular carboxylate dispersing agent, a sulfate dispersing agent and a nonionic block polyether wetting dispersing agent. Through screening of the dispersing agent and the thickening agent, the prepared suspending agent is high in stability, has a relatively good insecticidal effect on tea lesser leafhopper, and is efficient and safe in actual use.
Owner:HEMEISI (SHANDONG) PLANT PROTECTION CO LTD

Processing and preparation technology of cicada tea

A kind of tea processing preparation process, it is related to tea processing technical field, solve the problem of different water content of tea fresh leaves due to different degree of sucking and biting of small green leafhopper adults and nymphs and different time interval from sucking and biting to picking.The preparation process includes fresh leaf pretreatment, collision green and aroma enhancement, steam + high hot air fixation, spreading, striping, drying, screening and picking, aroma enhancement and packaging.In the fresh leaf pretreatment process, small green leafhopper adults are put in, and small green leafhoppers are attracted to suck and bite fresh leaves by yellow light.In the collision green process, tea fresh leaves are made to jump by vibration to carry out collision green treatment.The present application solves the problem of different degree of fresh leaf water loss due to different degree of sucking and biting of small green leafhopper adults and nymphs and different time interval from sucking and biting to picking by putting small green leafhopper adults and nymphs to concentrate sucking and biting of tea fresh leaves.Meanwhile, the quality of cicada tea is effectively improved by temperature and humidity control, light supplement, collision green and aroma enhancement, steam + high hot air fixation.
Owner:QIANSHAN COUNTY JINMA ECOLOGICAL AGRI DEV CO LTD +1

Insecticidal preparation based on rosemary-mint mixed oil as well as preparation method and application

The invention discloses an insecticidal preparation based on rosemary-mint mixed oil and a preparation method and application thereof, and belongs to the crossing field of green agriculture and biotechnology. The mixed essential oil is efficiently extracted through an ultrasonic-subcritical coupling extraction process, the rosemary oil extraction rate can reach 12.1%-12.4%, the menthol retention rate reaches 95.2%-97.1%, and the solvent residue is lower than 0.01 mg / kg; a full-plant-source ternary compound system (mixed essential oil, sophora alopecuroide extract and citrus peel oil) is designed, the synergistic coefficients of 50 formulas are all larger than or equal to 1.25, and six types of pests including thrips, red spiders, aphids, whiteflies, plutella xylostella and leafhoppers can be covered; a special application technology for eight types of crops (including tea leaves and traditional Chinese medicinal materials) is established, the quality of the crops reaches the standard after pesticide application, for example, the water extract of the tea leaves is increased by 1.2%, and the astragaloside content meets the standard of Chinese Pharmacopoeia 2020 edition; the storage stability is optimized, the control effect retention rate can reach 94.7% after 6 months of normal-temperature storage, and the requirement of 2-year industrial shelf life can be met; finally, the average reduction rate of the chemical pesticide is 90.3%, and the preparation cost is less than or equal to 80 yuan / kg. The composition can replace chemical pesticides and is suitable for green prevention and control of insect pests of fruits and vegetables, fields, commercial crops and traditional Chinese medicinal materials.
Owner:一毫米生物科技(杭州)有限公司

A viscous glue trap for controlling the tea green leafhopper

The utility model discloses a kind of viscose traps for preventing and treating tea small green leafhoppers, including support frame, install the insect sticking frame of support frame, install the insect sticking plate and first side extension plate of insect sticking frame, install the collection box of support frame, install the fixing nail of insect sticking frame, install the ground-inserting fixing part of support frame, ground-inserting fixing part has multiple interval setting barbs, insect sticking frame has integrally arranged second side extension plate, insect sticking plate is installed in insect sticking frame by fixing nail, collection box is located below insect sticking frame, first side extension plate and second side extension plate are matched and set, insect sticking plate, first side extension plate, fixing nail, second side extension plate and ground-inserting fixing part are multiple and interval setting, first side extension plate and second side extension plate are all set in inclined shape;Insect sticking frame is provided with multiple interval setting insect sticking grooves, insect sticking plate is placed in insect sticking groove, the depth of insect sticking groove is greater than the thickness of insect sticking plate.
Owner:FUJIAN LVFANG INTELLIGENT EQUIP CO LTD

A method for estimating the control period of tea green leafhopper based on automatic capture

The present invention provides a method for estimating the tea green leafhopper control period based on automatic capture, which relates to the field of tea pest control, comprising: step S1, active capture; step S2, identification and counting: using a recognition model to identify and count tea green leafhoppers in the image obtained in step S1, to obtain the number and average length of tea green leafhoppers; step S3, environmental data collection; step S4, pest control period prediction: using a prediction model completed through offline pre-training to predict the current tea green leafhopper control period, and correcting the prevention period result using a prediction time correction method. The method uses an automated system to accurately predict the pest outbreak period based on factors such as the number of tea green leafhoppers, insect length, temperature, and humidity, with high prediction accuracy and low error, effectively reducing dependence on professional technicians and avoiding the errors and lags caused by manual prediction.
Owner:CHONGQING ACAD OF AGRI SCI

An insecticidal microemulsion composition

ActiveCN119867064BBiocideAnimal repellantsLeafhopperBactericidin
This invention provides an insecticidal microemulsion composition of flonicamid and spinosad, which can be used to control various pests such as aphids, leafhoppers, planthoppers, and thrips, and is particularly effective against eggplant thrips. The addition of polyether-modified organosilicon as a synergist significantly enhances the efficacy of the flonicamid and spinosad microemulsion. The polyether-modified organosilicon also reduces the persistent foaming of the pesticide, preventing foaming and thus reducing its effectiveness.
Owner:SHANGHAI YUELIAN BIOLOGICAL TECH +1

Insecticide composition and preparation method thereof

The invention discloses an insecticide composition and a preparation method thereof, and relates to the technical field of pesticide compounding. According to the composition, Oxazosulfyl and beta-cypermethrin are compounded, a multifunctional modified white carbon black composite carrier serves as a carrier, and synergistic interaction is achieved through a micelle-slow release technology. The insecticide composition has quick-acting and persistent control effects on beet armyworms, ostrinia nubilalis, laodelphax striatellus and wheat aphids, is obviously superior to a single agent, and can delay the generation of resistance. The preparation process is simple, various dosage forms such as a suspending agent, an emulsion in water and the like can be processed, and good application prospects are achieved.
Owner:SHANDONG DAIMENDE BIOTECHNOLOGY CO LTD

Method for controlling insects

The present invention relates to a method for controlling insect infestation in stone fruits, the method comprising applying a nicotinic acetylcholine receptor (nAChR) competitive modulator to a locus or plant part of a plant or plant propagation material. The invention further relates to a method for controlling leafhopper infestation in mango cultivation or orchards using said nicotinic acetylcholine receptor (nAChR) competitive modulators.
Owner:UPL LTD

Preparation method of cowpea fruit fly repellent and application thereof

The application provides a preparation method of a cowpea leafhopper repellent and application thereof, and belongs to the technical field of green prevention and control of agricultural pests. The preparation method is as follows: activated biochar obtained by microwave pyrolysis of straw powder and activated by phosphoric acid is mixed with intercalated montmorillonite-PVA compound obtained by intercalating aluminum ions into montmorillonite and modifying the intercalated montmorillonite with polyvinyl alcohol, and then the mixture is modified with KH-550 to obtain a supported controlled-release carrier; linalool, geraniol and methyl salicylate are loaded on the supported controlled-release carrier to prepare a core; trans-2-hexenal and trans-2-octenal are loaded on the core to prepare a core-shell structure microsphere; the core-shell structure microsphere is coated with poly-N-isopropyl acrylamide to prepare a multi-stage controlled-release microsphere; and the multi-stage controlled-release microsphere is used to prepare the cowpea leafhopper repellent for preventing and controlling cowpea leafhoppers. The application improves the loading capacity and adsorption stability of active components, and improves the compatibility with the wax layer of cowpea leaves. The preparation of the application shows fast effect and long persistence.
Owner:SANYA RES INST OF CHINESE ACAD OF TROPICAL AGRI +1

Portable image counter for tea lesser leafhopper

The utility model discloses a portable image counter for tea lesser leafhopper, which relates to the technical field of pest detection and comprises a holding rod and a positioning block fixedly mounted on the left side of the holding rod, a control switch is fixedly mounted at the front end of the holding rod, and a charging port is arranged on the right side of the holding rod. And a telescopic rod is arranged in the left side of the positioning block. According to the portable image counter for the tea lesser leafhopper, the length of the telescopic rod can be adjusted according to needs, space is saved, stability is improved, the macro cameras can shoot clear images of smile insects such as the tea lesser leafhopper in a short distance, and the front macro camera and the back macro camera allow a user to capture images of the front face and the back face of tea at the same time; the LED light supplement can obtain a clear and consistent image during shooting, a flip screen is convenient to watch the image, the space is saved, the artificial intelligence identification algorithm can automatically and rapidly identify and count tea lesser leafhopper adults, nymphs and exudates, and the accuracy of field investigation results is improved.
Owner:CHINA JILIANG UNIV

A method for building a tea green leafhopper damage symptom detection model and its application

The present invention discloses a method for building a tea green leafhopper damage symptom detection model and its application. The building method comprises the following steps: Step 1: Acquire a tea bud image dataset with different degrees of damage caused by the tea green leafhopper; Step 2: Preprocess the acquired tea bud image datasets respectively; Step 3: Build a tea green leafhopper damage symptom detection model and perform model evaluation; Step 4: Train and test the model built in step 3 to obtain a deep learning model for tea green leafhopper damage symptom detection. The present invention also provides a method for tea green leafhopper damage symptom detection and a device for tea green leafhopper damage symptom detection. The present invention reveals the tea green leafhopper damage symptom classification potential based on RGB and hyperspectral imaging, and provides a necessary technical approach for the construction of smart tea gardens. At the same time, the present invention also provides a specific reference for accurate, efficient and non-destructive monitoring of other crop diseases and insect pests.
Owner:QINGDAO AGRI UNIV

RdDH3 gene and application thereof in preparation of product for inhibiting in-vivo virus replication of electro-optic leafhopper

The invention belongs to the technical field of agricultural biology, and particularly provides a gene RdDH3 of electro-optic leafhopper, and the nucleotide sequence of the gene RdDH3 is shown as SEQ ID NO.1. The invention proves that the replication level of the RSMV in the electro-optic leafhopper can be obviously reduced by silencing the electro-optic leafhopper RdDH3 gene, so that an important theoretical basis is provided for developing a molecular target for preventing and controlling the rice stripe mosaic disease. Besides, specific dsRNA is developed for the RdDH3 gene, and the dsRNA can remarkably inhibit replication of RSMV in the electro-optic leafhopper, so that the transmission capacity of the RSMV in the electro-optic leafhopper is weakened, and finally effective prevention and control of the rice stripe mosaic disease are achieved. The dsRNA disclosed by the invention has the advantages of high specificity, environmental friendliness and no toxicity or harm to human beings, can be applied to agricultural production as a new generation of RNA pesticide, and shows wide application prospects and important application values.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Leafhopper hemolymph collection device

This utility model relates to the field of insect hemolymph collection technology, and discloses a leafhopper hemolymph collection device, including a collection tube, a rubber stopper at the opening of the collection tube, a negative pressure cylinder, a liquid collection tube with an insertion needle at one end, and a positioning component for positioning the collection tube. The other end of the liquid collection tube passes through the rubber stopper and is connected to the collection tube. The rubber stopper is tightly fitted to the opening of the collection tube. An air inlet is opened at the upper part of the negative pressure cylinder, and a bent tube is fixedly inserted into the air inlet of the negative pressure cylinder. The bent tube is fixedly inserted into the rubber stopper and is connected to the collection tube. The positioning component includes an upper sleeve with an opening at the lower end, a lower sleeve with an opening at the upper end, and multiple arc-shaped positioning blocks made of elastic rubber. The purpose is to provide a device for collecting leafhopper hemolymph and to solve the problem that existing insect hemolymph extraction devices require insertion and removal of the suction hose and liquid collection hose on the sealing cap during use, resulting in a complex structure and inconvenience in use.
Owner:YULIN UNIV +1

Cyctria megacephala odorant binding protein and application thereof

According to the odorant binding protein for the black-tailed leafhopper and the application of the odorant binding protein, by interfering the expression quantity of the binding protein in the black-tailed leafhopper, the perceptual ability of the black-tailed leafhopper to volatile matters induced by rice dwarf viruses can be effectively inhibited, so that the effect of preventing and treating the rice dwarf viruses is achieved. The amino acid sequence of the odorant binding protein is SEQ ID NO: 1. According to the application disclosed by the invention, a fluorescent competitive binding experiment finds that the odorant binding protein NcinOBP7 of the black tail leafhopper and a volatile matter released by rice induced by a rice dwarf virus have high affinity; an RNAi experiment shows that the rice volatile matter induced by the rice dwarf virus has an attraction effect on the black-tail leafhopper nymphs injected with dsEGFP, but after the NcinOBP7 is silenced, the rice volatile matter does not attract the black-tail leafhopper nymphs any more. Therefore, the silence of the black-tail leafhopper NcinOBP7 can influence the perception of the black-tail leafhopper on the rice volatile matters, and subsequently speculates to inhibit the feeding of the black-tail leafhopper on the virus-infected rice and influence the transmission of viruses.
Owner:QINGDAO AGRI UNIV

Biopesticide for preventing and treating tea lesser leafhopper

PendingCN120615934ABiocideAnimal repellantsSporelingOxymatrine
The invention belongs to the technical field of biopesticides, and particularly relates to a biopesticide for preventing and treating tea lesser leafhopper, the active component of the biopesticide is formed by compounding metarhizium anisopliae and oxymatrine or tenvermectin; wherein the metarhizium anisopliae is a metarhizium anisopliae CQMa421 mother drug with a concentration of 15 billion spores per gram. According to the invention, the microbe source metarhizium anisopliae with insecticidal activity is reasonably compounded with other biopesticide components, and compared with the singly used source metarhizium anisopliae, the control effect on tea lesser leafhopper can be improved.
Owner:SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI

Traditional Chinese medicine compound preparation for green prevention and control of cowpea thrips

The invention discloses a traditional Chinese medicine compound preparation for green prevention and control of cowpea thrips, and relates to the technical field of cowpea thrip prevention and control. According to the preparation disclosed by the invention, various traditional Chinese medicines such as monarch medicines, ministerial medicines, assistants and guides of traditional Chinese medicines are used for synergistically preventing and controlling cowpea thrips, contact toxicity, stomach toxicity and avoidance are generated on cowpea thrips to interfere growth activities, and green prevention and control effects are generated on the thrips at multiple sites; pesticide residues caused by chemical pesticides can be eliminated, real zero pesticide residues are achieved, and the standard of green ecology and organic vegetables is achieved; the efficient, safe and environment-friendly traditional Chinese medicine compound insecticidal preparation is creatively developed by deeply fusing the traditional Chinese medicine theory, principle essence and modern plant protection science, not only has outstanding creativity and novelty in formula and action mechanism, but also shows strong practicability in raw material, production, use and effect, and has a wide application prospect. The invention provides an overall solution technical scheme with great market prospect and technical value for sustainable treatment of cowpea thrips and development of green ecological agriculture.
Owner:CHENGDU LIANGSHAN AGRICULTURAL ENGINEERING TECHNOLOGY RESEARCH INSTITUTE

A leafhopper monitoring and trapping device with a leafhopper trapping function

The utility model discloses a kind of with leafhopper luring and killing function's leafhopper monitoring trapping device, belong to leafhopper trapping field, including located at top's protective cover, located in middle's double section light source trapping connecting cylinder, located at bottom's support cylinder seat, solar panel is provided on protective cover, the inside of protective cover is provided for monitoring leafhopper trapping condition's monitoring sensing module, double section light source trapping connecting cylinder includes the main light source trapping cylinder for trapping leafhopper inside hollow, the inside hollow auxiliary light source trapping cylinder for trapping leafhopper, the top of main light source trapping cylinder is connected with protective cover by magnetic attraction structure.
Owner:BAOTING GOLDEN POMEGRANATE AGRICULTURE CO LTD

Rice stripe mosaic virus expression vector, gene silencing vector, preparation method and application

The invention relates to the technical field of gene engineering, and discloses a rice stripe mosaic virus (RSMV) expression vector, a gene silencing vector, a preparation method and application. The rice stripe mosaic virus vector composition provided by the invention comprises a construction body used for transcriptional generation of virus anti-genome RNA, a construction body used for expressing trans-acting protein necessary for virus replication and transcription, and a construction body used for expressing a virus silencing suppressor. By utilizing the combination of the components, the recombinant RSMV with infection activity can be obtained in nicotiana benthamiana cells, and the recombinant virus can be further introduced into a mediator insect electro-optic leafhopper through micro-injection and is inoculated to a rice plant through the electro-optic leafhopper to establish systemic infection. The invention provides application of the recombinant virus vector in expression of foreign proteins or silence of endogenous genes in electro-optic leafhoppers and rice bodies and the like. The vector has the advantages of strong exogenous fragment bearing capacity, high genetic stability and the like.
Owner:ZHEJIANG UNIV

A method for monitoring the control of insect pests in tea plantations

The present application relates to a kind of tea tree planting in the monitoring method of pest control, it includes the following steps: a, first adopt main network to carry out feature extraction to image sample, shallow feature of image sample is simultaneously selected and regression operation is carried out;B, the deep feature in each layer of main network is gradually stacked to shallow feature by the way of up-sampling and convolution layer combination in each layer;C, the deep feature of each layer of main network is fused with the shallow feature of each layer after corresponding stacking again, as the output layer of neural network, and complete the regression identification of tea green leafhopper;Meanwhile, according to the identification and regression operation result combination demarcated yellow board range, complete the deduplication processing of the same position tea green leafhopper repeated identification on different output layer, finally quantity is calculated.The monitoring method can efficiently complete the identification, counting of tea green leafhopper, and the judgment accuracy is high, time-saving and labor-saving, saves manual cost.
Owner:CHONGQING ACAD OF AGRI SCI

Dynamic prediction method of tea green leafhopper pest based on meteorological and insect monitoring data

The present invention discloses a method for dynamic prediction of tea green leafhopper pests based on meteorological and insect monitoring data. The method comprises the following steps: Step 1, obtaining data and constructing a model data set. Step 2, calculating the pest pressure index. The pest pressure index includes a pressure value and a degree value. The pressure value is a comprehensive pressure value of the occurrence of tea green leafhopper pests at the current time and the future pest development trend; the degree value is the risk level of the occurrence of tea green leafhopper pests at the current time and the future pest development trend. Step 3, according to the size of the pressure value and the degree value, the risk of pest outbreak in the measured area at present and in the next few days is evaluated. The present invention is based on the historical green leafhopper pest pressure in the measured area, combined with meteorological data and insect population density monitoring data, to obtain the risk of tea green leafhopper pest occurrence on the current day and in the next few days in real time.
Owner:HANGZHOU DIANZI UNIV

Method and special kit for rapidly detecting sodium ion channel K1774N mutation of cowpea megthrips

The invention discloses a method and a special kit for rapidly detecting sodium ion channel K1774N mutation of methrips vigna, and belongs to the technical field of biological detection. The method mainly comprises the following steps: 1) extracting DNA (Deoxyribose Nucleic Acid) of the cowpea 2) carrying out PCR amplification by adopting two groups of primer pairs; (3) result judgment: when a 269bp band appears in amplification of the first group of primer pairs, the detected sodion channel gene mutation site K1774N of the cowpea megthrips spp sample is not mutated; when a 269bp band appears in amplification of the second group of primer pairs, the detected sodion channel gene mutation site K1774N of the cowpea megaluthrips spp sample is mutated, and when no band appears, the detected cowpea megaluthrips spp sample is not mutated. According to the application, the mutation condition of the field cowpea megthrip K1774N mutation site can be quickly and effectively detected, and the real-time state of pyrethroid resistance of the field cowpea megthrip is effectively detected.
Owner:HAINAN UNIVERSITY SANYA NANFAN RESEARCH INSTITUTE

A method for large-scale breeding and preservation of tea green leafhoppers suitable for cicada tea production.

ActiveCN119423022BLeafhopperZoology
This invention discloses a method for large-scale breeding and preservation of the tea green leafhopper suitable for cicada tea production. Step 1: Select Jin Xuan, Cui Yu, or Fuding Da Bai tea as the host plant variety and determine the host plant specifications; Step 2: Establish adult tea green leafhopper breeding areas, nymph breeding areas, and egg incubation areas, and breed in separate zones, optimizing the optimal breeding conditions and stocking density for each zone; Step 3: Optimize the method for collecting tea green leafhopper eggs, including optimizing the adult sex ratio, egg-laying density, and egg collection time; Step 4: Establish a tea green leafhopper egg storage room and optimize the storage conditions; Step 5: Optimize the incubation conditions and incubation time for tea green leafhopper eggs after low-temperature storage. This invention's large-scale production method utilizes unpruned tea branches from tea gardens, resulting in low costs. Using tea branches for breeding reduces the mortality rate of leafhoppers needing to readjust to the host plant upon release.
Owner:INST OF ZOOLOGY GUANGDONG ACAD OF SCI

Method for artificially feeding tea lesser leafhopper egg parasitic wasps by using brown planthopper eggs and application

The invention discloses a method for artificially feeding tea lesser leafhopper egg parasitic wasps by using brown planthopper eggs and application, and relates to the technical field of biological prevention and control of agricultural pests. By utilizing the advantages that brown planthopper eggs and tea lesser leafhopper eggs are similar in size and development condition, and the brown planthopper eggs are easier to produce in batches, the tea lesser leafhopper egg parasitic wasps can actively parasitize the brown planthopper eggs through step-by-step domestication, and the offspring of the parasitic wasps can be developed and eclosed into adults in the brown planthopper eggs, so that the survival rate of the tea lesser leafhopper eggs is increased, and the survival rate of the tea lesser leafhopper eggs is increased. The possibility is provided for artificial batch feeding of tea lesser leafhopper egg parasitic wasps.
Owner:福建省农业科学院水稻研究所 +1

Tolfenpyrad-containing environment-friendly pesticide

The invention relates to the technical field of pesticides, and discloses an environment-friendly pesticide containing tolfenpyrad. The environment-friendly pesticide comprises effective active components, a biological synergist and an auxiliary agent, and is prepared into a suspending agent. The effective active components are deltamethrin and tolfenpyrad, and the ratio of deltamethrin to tolfenpyrad is (1: 6)-(1: 12). The biological synergist is a biological enzyme, a microbial metabolite or amino acid. The environment-friendly pesticide provided by the invention can effectively prevent and control tea lesser leafhoppers of tea trees, can effectively control the occurrence of pests, improves the utilization rate of the environment-friendly pesticide, reduces pesticide residues and pesticide application times, and also can improve the quality of crops.
Owner:SHAANXI THOMPSON BIOTECHNOLOGY CO LTD