6 results about "Phytoplasma" patented technology

A new and distinct cultivar of Poinsettia plant named ‘Doeuprerib’, characterized by its uniform, upright and mounded plant habit; moderately vigorous growth habit; freely and upright branching habit without requiring phytoplasma infection; large dark green-colored leaves; plants flower on or about November 25 in Southern California under natural season conditions; small inflorescences with dark red-colored flower bracts; and good post-production longevity.

The invention belongs to the technical field of immunity and molecular biology, and relates to a phage display shark source single-domain antibody targeting areca phytoplasma and application of the phage display shark source single-domain antibody. The amino acid sequence of the phage display shark source single-domain antibody is shown as any one of SEQ ID NO.1-SEQ ID NO.2. The phage display shark source single-domain antibody is small in molecular weight, easy to modify and capable of deeply recognizing hidden antigen sites, and the phage display shark source single-domain antibody can be applied to the field of areca phytoplasma. The areca phytoplasma immunodominant membrane protein has high stability, and has high affinity and specificity to the areca phytoplasma immunodominant membrane protein. The phage display shark source single domain antibody provided by the invention can be used for detecting areca phytoplasma, has the advantages of rapid detection, high sensitivity, low interference, high stability and quantitative detection, and can realize on-site rapid detection while realizing detection of trace level phytoplasma immune dominant membrane protein in areca.

The invention belongs to the technical field of agricultural biological protection, and discloses a targeted screen tube-vector insect lysozyme-nano magnesium compound system and application thereof in prevention and control of areca yellows, the compound system comprises an in-plant treatment module and a propagation blocking module, the in-plant treatment module comprises a screen tube targeted delivery unit, and the propagation blocking module comprises a propagation blocking unit. The sieve tube targeted delivery unit is formed by combining betel nut sieve tube navigation peptide ArePEP1 and a lysozyme-nano magnesium complex through a non-covalent bond; the propagation blocking module comprises an antifeedant micro-capsule activated by salivary enzyme of a vector insect, and phytoplasma deoxyribozyme PdeA and an insect antifeedant are encapsulated in the antifeedant micro-capsule; the target sequence of the phytoplasma deoxyribozyme PdeA is 5 '-GGAAACAG-3'; the in-plant treatment module and the propagation blocking module are jointly loaded on the same delivery vehicle. According to the areca yellows prevention and control compound system and the application method, pathogens in plants can be synchronously inhibited, insect propagation can be blocked, and high-altitude accurate delivery can be realized.

This invention discloses a qPCR primer-probe combination, kit, and method for specifically detecting 16SrII group areca etiolated phytoplasma, belonging to the interdisciplinary field of molecular biology and plant pathology. The primer-probe combination includes a forward primer qTuf-F4-2 (SEQ ID NO:1), a reverse primer qTuf-R4 (SEQ ID NO:2), and a fluorescent probe qTuf-P4-1 (SEQ ID NO:3), targeting 16SrII group phytoplasma. tuf This gene conserved region demonstrates high specificity for 16SrII group areca yellowing phytoplasma in qPCR reactions, exhibiting no cross-reactivity with 16SrI group, 16SrXXXII group phytoplasma, areca genome, common pathogens, or endophytes. The limit of detection is 14.35 copies / μL, and the sensitivity meets the requirements for detecting low-load samples. The coefficient of variation for intra- and inter-group repeatability is less than 1%, indicating good stability. The kit and detection method developed based on this combination are simple to operate, highly sensitive, and provide stable results, making them suitable for the accurate diagnosis, pathogen typing, and field epidemic monitoring of areca yellowing disease, providing key technical support for the scientific control of 16SrII group phytoplasma.

The application discloses a double real-time fluorescent quantitative TaqMan primer probe set, a kit and a detection method for simultaneously detecting sweet potato leaf curl virus and sweet potato witches' broom phytoplasma, which is designed according to the nucleotide sequences of SPLCV and SPWB genome sequencing in a conservative segment, and is used for specific detection of SPLCV and SPWB; and the application also designs a fluorescent quantitative kit based on the primer probe set. The primer and probe set can simultaneously detect the target of SPLCV and SPWB in one reaction, is fast and convenient, has high detection efficiency, and has high detection sensitivity of 3.3 copies / uL, which is 100 times of that of ordinary PCR. The TaqMan QPCR detection technology established by the application for SPLCV and SPWB solves the problems of time-consuming and labor-consuming, poor specificity and sensitivity, and easy pollution in the existing detection methods of SPLCV and SPWB, and provides a technical basis for early diagnosis of SPLCV and SPWB.