Traditional Chinese medicine compound preparation for green prevention and control of cowpea thrips

Abstract

The invention discloses a traditional Chinese medicine compound preparation for green prevention and control of cowpea thrips, and relates to the technical field of cowpea thrip prevention and control. According to the preparation disclosed by the invention, various traditional Chinese medicines such as monarch medicines, ministerial medicines, assistants and guides of traditional Chinese medicines are used for synergistically preventing and controlling cowpea thrips, contact toxicity, stomach toxicity and avoidance are generated on cowpea thrips to interfere growth activities, and green prevention and control effects are generated on the thrips at multiple sites; pesticide residues caused by chemical pesticides can be eliminated, real zero pesticide residues are achieved, and the standard of green ecology and organic vegetables is achieved; the efficient, safe and environment-friendly traditional Chinese medicine compound insecticidal preparation is creatively developed by deeply fusing the traditional Chinese medicine theory, principle essence and modern plant protection science, not only has outstanding creativity and novelty in formula and action mechanism, but also shows strong practicability in raw material, production, use and effect, and has a wide application prospect. The invention provides an overall solution technical scheme with great market prospect and technical value for sustainable treatment of cowpea thrips and development of green ecological agriculture.

Description

Technical Field

[0001] This invention relates to the field of cowpea thrips control technology, specifically to a traditional Chinese medicine compound preparation for the green control of cowpea thrips. Background Technology

[0002] During the growth period of cowpeas, a vegetable grown in southern China, thrips are a common pest during the seedling, flowering, and pod-setting stages. Thrips damage the leaves, buds, and young pods of cowpeas. If the damage continues, the infestation can lead to leaf damage, bud drop, and even the death of the entire plant. Thrips also impair photosynthesis, severely impacting the yield and quality of cowpeas, resulting in a significant reduction in crop production.

[0003] Existing methods for controlling cowpea thrips mostly involve pesticide application. However, due to the short production cycle and frequent harvesting of cowpeas, farmers apply pesticides very frequently, often using and abusing various chemical pesticides. Over time, cowpea thrips have developed resistance, making them even more difficult to control. Furthermore, the short production-to-sale cycle of cowpeas leaves insufficient safety intervals. Cowpea thrips are small and adept at hiding in concealed places such as flower parts, growing points, and the undersides of leaves, making it difficult for them to come into contact with the pesticide solution, thus failing to achieve the desired control effect. Therefore, we propose a traditional Chinese medicine compound preparation for the green control of cowpea thrips. Summary of the Invention

[0004] The purpose of this invention is to provide a traditional Chinese medicine compound preparation for the green control of cowpea thrips, in order to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention specifically adopts the following technical solution:

[0006] A traditional Chinese medicine compound preparation for the green control of cowpea thrips, the traditional Chinese medicine compound preparation comprising the following raw materials by weight:

[0007] Sophora flavescens, 20-30 parts;

[0008] Veratrum nigrum, 20-25 parts;

[0009] Sichuan pepper, 10-15 parts;

[0010] 10-15 doses of Stemona japonica;

[0011] Soapberry, 5-10 parts;

[0012] Peppermint, 5-10 parts;

[0013] Licorice, 5-10 parts.

[0014] Furthermore, the preparation method of the traditional Chinese medicine compound preparation includes the following steps:

[0015] S1. Raw material preparation: Prepare Sophora flavescens, Veratrum nigrum, Melia toosendan, Stemona japonica, Gleditsia sinensis, Mentha haplocalyx, and Glycyrrhiza uralensis according to the above weight proportions;

[0016] S2. Raw material pretreatment: Remove impurities from the prepared raw materials, wash them, cut Sophora flavescens, Veratrum nigrum, Melia toosendan and Stemona japonica into small sections, crush Gleditsia sinensis, and keep the peppermint and licorice root intact for later use.

[0017] S3. Crushing: Crush the pre-treated raw materials into fine powder using a high-speed crusher to ensure that the material is uniform and delicate.

[0018] S4. Mixing and sieving: Put the pulverized fine powder into the mixing equipment and stir at low speed to ensure that the powder is fully and evenly mixed and to avoid clumping; sieve the evenly mixed powder through a sieve to remove large particles and ensure that the fineness of the preparation is consistent.

[0019] S5. Cooking: Place the mixed and sieved ingredients in a stainless steel saucepan, add purified water and bring to a boil over high heat, then reduce to a simmer and stir occasionally.

[0020] S6. Cooling: Let the prepared liquid stand and cool, avoiding direct sunlight or violent shaking during the process to maintain the stability of the active ingredients.

[0021] S7. Adding microbial agents to the cooled stock solution and place it in a sterile fermentation tank for fermentation.

[0022] S8. Negative pressure filtration: Transfer the fermented liquid to a sterile negative pressure filtration device, apply negative pressure, and filter to remove residual bacteria, particulate impurities, and large molecular precipitates; after filtration, collect the clear and transparent filtrate to obtain the Chinese herbal medicine liquid.

[0023] S9. Preparation: Dilute the obtained Chinese herbal extract with purified water and mix at low speed using a stirrer to ensure uniformity and no sedimentation; at the same time, adjust the pH value to keep the active ingredients stable; after preparation, a clear liquid preparation that can be directly used for spray application is obtained and stored in a light-proof container for later use.

[0024] Furthermore, the fineness of the powder in the pulverizing step is 80-100 mesh.

[0025] Furthermore, in the mixing and sieving step, the low-speed stirring speed is 200-300 rpm, and the stirring time is 15-20 minutes.

[0026] Furthermore, in the cooking step, the ratio of fine powder to water is 1:10, and the cooking time is 2 to 3 hours.

[0027] Furthermore, the cooling temperature in the cooling step is 25–35°C.

[0028] Furthermore, the fermentation time in the bacterial fermentation step is 5 to 7 days, and the microbial agent is selected from beneficial microbial strains such as lactobacillus or brewer's yeast.

[0029] Furthermore, the negative pressure value in the negative pressure filtration step is 0.05 to 0.1 MPa; the filtration is carried out using a filter membrane with a pore size of 0.45 micrometers.

[0030] The beneficial effects of this invention are as follows:

[0031] 1. The control effect of this invention is 25-30% higher than that of chemical pesticides. Comparative tests show that chemical pesticides have a control effect of 60-70% on cowpea thrips, while the green control formulation has an effect of over 90%.

[0032] 2. This invention solves the problem of cowpea thrips resistance to chemical pesticides. The formulation uses a combination of traditional Chinese medicines, including principal, assistant, and adjuvant herbs, to synergistically control cowpea thrips. It exerts contact toxicity, stomach poisoning, and repulsive interference on the growth activities of cowpea thrips, providing green control at multiple sites.

[0033] 3. This invention can eliminate pesticide residues caused by chemical pesticides, achieving true zero pesticide residues and meeting the standards of green, ecological, and organic vegetables.

[0034] 4. This invention creatively develops a highly efficient, safe, and environmentally friendly compound insecticide using traditional Chinese medicine by deeply integrating the essence of traditional Chinese medicine theory and principles with modern plant protection science. It not only possesses outstanding creativity and novelty in its formulation and mechanism of action, but also demonstrates strong practicality in terms of raw materials, production, use, and effects, providing a comprehensive technical solution with significant market potential and technological value for the sustainable management of cowpea thrips and the development of green ecological agriculture. Attached Figure Description

[0035] Figure 1 This is a flowchart illustrating the preparation process of the traditional Chinese medicine compound preparation in this invention. Detailed Implementation

[0036] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings.

[0037] This invention provides a traditional Chinese medicine compound preparation for the green control of cowpea thrips. The traditional Chinese medicine compound preparation comprises the following raw materials by weight:

[0038] Sophora flavescens, 20-30 parts; Sophora flavescens contains matrine and oxymatrine, which have strong contact and stomach poison effects.

[0039] Veratrum nigrum, 20 - 25 parts; Veratrum nigrum contains protoveratrine A and protoveratrine B, which have strong insecticidal and pharmacological activities.

[0040] Taking Sophora flavescens and Veratrum nigrum as monarch drugs; The insecticidal activity of Sophora flavescens mainly comes from various alkaloids it contains, among which matrine is the most important insecticidal active ingredient.

[0041] Oxymatrine, usually coexisting with matrine, also has significant biological activities. Sophocarpine, oxysophocarpine, and sophoridine play a synergistic insecticidal role. Matrine and the ester alkaloids, which are insecticidal active ingredients in Veratrum nigrum, and the steroidal alkaloids with strong physiological activities jointly contact and stomach - kill thrips. The cevadine - type alkaloids in the roots of Veratrum nigrum, with protoveratrine A and protoveratrine B as representative components, are the most important and have the strongest insecticidal activities in Veratrum nigrum. Under alkaline or enzymatic hydrolysis conditions, they will hydrolyze to form more toxic veratrylamine.

[0042] The cevadine - type alkaloids in Veratrum nigrum, with saceine, veratrine, etc. as representative components, also have strong insecticidal and pharmacological activities.

[0043] Toosendan fruit, 10 - 15 parts; Toosendan fruit contains azadirachtin, toosendanin, and melia azedarachin, assisting the monarch drugs in contact killing, stomach poisoning, and antifeedant effects.

[0044] Stemona root, 10 - 15 parts; Stemona root contains tuberostemonine, stemine, etc. as insecticidal components, which can assist the monarch drugs in enhancing the insecticidal efficacy of contact killing, stomach poisoning, antifeedant, molting inhibition, and interfering with growth, reproduction, and breeding of thrips.

[0045] Taking Toosendan fruit and Stemona root as ministerial drugs; The insecticidal active components of Toosendan fruit are a class of highly oxidized limonoids. Among them, the most important and core component is azadirachtin, which is the most insecticidal monomer compound in Toosendan. In addition to azadirachtin, Toosendan also contains other various limonoids, which act synergistically with azadirachtin to jointly form a powerful insecticidal spectrum of Toosendan, including: toosendanin, melia azedarachin, azadirachtin analogs: such as salanin, nimbinin, etc. They also have significant biological activities.

[0046] The core insecticidal effective components of Stemona root are Stemona alkaloids: Tuberostemonine is one of the main active components with relatively high content and in - depth research in Stemona. Stemine, protostemine, sessilistemonine, and mazusoline. Most of these alkaloids have complex ring structures, and they jointly constitute the chemical basis of Stemona's insecticidal effect on thrips. Through synergistic effects, they have various physiological effects on thrips.

[0047] The azadirachtin, toosendanin, and melia azedarachin in Toosendan fruit and the tuberostemonine, stemine, etc. in Stemona root can assist the monarch drugs Sophora flavescens and Veratrum nigrum in enhancing the insecticidal efficacy of contact killing, stomach poisoning, antifeedant, molting inhibition, and interfering with growth, reproduction, and breeding of thrips.

[0048] Gleditsia sinensis, 5-10 parts; Gleditsia sinensis contains saponins, a "super adjuvant" that enhances the insecticidal effects of principal and assistant herbs (such as Sophora flavescens, Veratrum nigrum, Melia toosendan, Stemona japonica, etc.) in multiple ways: it has strong penetrating and repellent effects.

[0049] Peppermint, 5-10 parts; peppermint contains menthol and menthone, has a strong odor, good repellent effect, and can enhance penetration.

[0050] Licorice, 5-10 parts; licorice harmonizes the various herbs, acts as a surfactant and synergist, and improves the adhesion and stability of the medicinal solution.

[0051] Gleditsia sinensis, peppermint, and licorice are used as adjuvant herbs. The core active ingredient of Gleditsia sinensis is a class of natural surfactants called saponins. Triterpenoid saponins are the most abundant and most active type of saponin in Gleditsia sinensis. Steroidal saponins also possess surface activity and biological activity. Saponins in Gleditsia sinensis can significantly enhance the auxiliary insecticidal effects of the principal and assistant herbs.

[0052] Soapberry itself has an auxiliary insecticidal effect. Even before being used as an adjuvant, soapberry itself already possesses a certain degree of direct insecticidal ability.

[0053] Contact killing effect on thrips: Saponins can destroy the waxy layer and epidermal structure of thrips, causing a large amount of water to evaporate from the insect's body. At the same time, saponin components can also penetrate into the insect's body through the damaged epidermis, interfering with normal physiological functions.

[0054] Stomach poisoning: Saponins have an irritating and destructive effect on the digestive tract of pests. They are particularly effective against target pests such as thrips, spider mites, and aphids, which have thin-walled piercing-sucking mouthparts.

[0055] The adjuvant herb Gleditsia sinensis plays the role of a "super adjuvant" or "guide herb," ​​greatly enhancing the insecticidal effects of principal and assistant herbs (such as Sophora flavescens, Veratrum nigrum, Melia toosendan, Stemona japonica, etc.) through various means.

[0056] Excellent wetting and spreading properties. Plant leaves (especially legumes like cowpeas) have a waxy layer on their surface, making them hydrophobic. Ordinary pesticide sprays will form water droplets that roll off, resulting in small coverage areas and poor adhesion. The role of saponins: Saponins are highly effective natural surfactants that greatly reduce the surface tension of pesticide solutions, allowing them to spread rapidly on leaves and insects, forming a uniform film, increasing the contact area, preventing pesticide roll-off, and significantly improving pesticide utilization.

[0057] Powerful penetration and adhesion: Thrips, especially thrips nymphs, have a protective waxy layer on their body surface. The main active ingredients in pesticides (such as matrine and veratrine) have difficulty penetrating this layer. Saponins in soapberry can dissolve and destroy this waxy protective layer, opening channels for the active ingredients of the pesticide to penetrate more easily into the thrips' bodies, thus significantly improving contact toxicity and speed of action. Simultaneously, it also enhances the adhesion of the pesticide to plants and thrips, making it resistant to rain washout.

[0058] Emulsification and Dispersion: Active ingredients extracted from traditional Chinese medicine (such as alkaloids and azadirachtin) may be insoluble in water or easily precipitate, leading to uneven spraying and affecting efficacy. The hydrophilic and lipophilic properties of saponin molecules can emulsify and stably disperse other lipid-soluble active ingredients in water, forming a uniform and stable solution, ensuring that every drop contains sufficient active ingredients.

[0059] Synergistic effect mechanism: The insecticidal effect of soapberry itself (destroying the body wall) complements the neurotoxic and stomach toxic effects of the principal and assistant drugs. It first destroys the first physical defense line of thrips pests, allowing the main active ingredients of the principal and assistant drugs to "penetrate deeply" and achieve a synergistic insecticidal effect of "1+1>2".

[0060] Licorice harmonizes the effects of other herbs, and its glycyrrhizic acid component enhances the adhesion and spreadability of medicinal solutions on plant surfaces, improving utilization. Peppermint volatile oil acts as a natural penetration aid, carrying active ingredients through the waxy layer of cowpea leaves to reach the affected area directly. Simultaneously, the seedling-strengthening effects of licorice and the liver-soothing and mood-lifting properties of peppermint help alleviate thrips damage stress and promote cowpea recovery and growth.

[0061] This formulation does not act solely as an insecticide, but rather constructs a triple protective network of "direct insecticidal action + biological barrier + growth regulation." This multi-pronged approach significantly reduces the risk of cowpea thrips developing resistance and overcomes the inherent limitations of single-mechanism chemical pesticides.

[0062] Please see Figure 1 The preparation method of traditional Chinese medicine compound preparations includes the following steps:

[0063] S1. Raw material preparation: Prepare Sophora flavescens, Veratrum nigrum, Melia toosendan, Stemona japonica, Gleditsia sinensis, Mentha haplocalyx, and Glycyrrhiza uralensis according to the above weight proportions;

[0064] S2. Raw material pretreatment: Remove impurities from the prepared raw materials, wash them, cut Sophora flavescens, Veratrum nigrum, Melia toosendan and Stemona japonica into small sections, crush Gleditsia sinensis, and keep the peppermint and licorice root intact for later use.

[0065] S3. Crushing: The pre-treated raw materials are crushed using a high-speed crusher to fine powder to ensure that the material is uniform and fine. The fineness of the powder is 80-100 mesh.

[0066] S4. Mixing and sieving: Put the pulverized fine powder into the mixing equipment and stir at a low speed (200-300 rpm, stirring time 15-20 minutes) to ensure that the powder is fully mixed and uniform, and to avoid clumping; sieve the uniformly mixed powder through a sieve to remove large particles and ensure that the fineness of the preparation is consistent.

[0067] S5. Cooking: Place the mixed and sieved ingredients in a stainless steel saucepan, add purified water (the ratio of fine powder to water is 1:10), bring to a boil over high heat (cooking time is 2-3 hours), then reduce to a simmer and stir occasionally.

[0068] S6. Cooling: Let the prepared stock solution stand and cool (cooling temperature is 25-35℃). Avoid direct sunlight or violent shaking during this period to maintain the stability of the active ingredients.

[0069] S7. Adding microbial agents (using beneficial microbial strains such as lactobacillus or brewer's yeast) to the cooled stock solution and place it in a sterile fermentation tank for fermentation (fermentation time is 5 to 7 days).

[0070] S8. Negative pressure filtration: Transfer the fermented liquid to a sterile negative pressure filtration device, apply negative pressure (0.05-0.1 MPa), and filter (using a filter membrane with a pore size of 0.45 microns) to remove residual bacteria, particulate impurities, and large molecular precipitates; after filtration, collect the clear and transparent filtrate to obtain the Chinese herbal medicine liquid.

[0071] S9. Preparation: Dilute the obtained Chinese herbal extract with purified water and mix at low speed using a stirrer to ensure uniformity and no sedimentation; at the same time, adjust the pH value to keep the active ingredients stable; after preparation, a clear liquid preparation that can be directly used for spray application is obtained and stored in a light-proof container for later use.

[0072] Experimental Example 1

[0073] In 2024, a cowpea planting base in Ledong County, Hainan Province, conducted a comparative experiment on the control of cowpea thrips using chemical pesticides and formulations, covering an area of ​​3 mu (approximately 0.2 hectares). The chemical pesticide experiment was compared with a comparative experiment using the interleaved method. There were three replicates, with each replicate covering 0.5 mu (approximately 0.067 hectares). Chemical pesticides were applied alternately from cowpea emergence onwards using thiamethoxam, acetamiprid, cyhalothrin, and bifenthrin. Formulations were sprayed every 5-7 days, with each application using 200-300 ml diluted in 20-30 liters of water for foliar spraying. The total usage per mu was 8-9 liters.

[0074] Experimental results: Chemical pesticides were 60-70% effective in controlling cowpea thrips. The traditional Chinese medicine ginseng and asparagus root achieved over 90% effectiveness in controlling cowpea thrips.

[0075] Experimental Example 2

[0076] A cowpea planting base in Miyi County, Panzhihua City, Sichuan Province, conducted a comparative experiment on the control of cowpea thrips using chemical pesticides and formulations from 2024 to 2025, covering an area of ​​4 mu (approximately 0.67 hectares). The chemical pesticide experiment was compared with a comparative experiment using the interleaved method. There were four replicates, with each replicate covering 0.5 mu (approximately 0.067 hectares). Chemical pesticides were applied alternately from cowpea emergence onwards using bromufenamide, thiamethoxam, acetamiprid, and bifenthrin. Formulations were sprayed every 5-7 days, with each application using 200-300 ml diluted in 20-30 liters of water for foliar spraying. The total usage per mu was 8-9 liters.

[0077] Experimental results: Chemical pesticides were 65-70% effective in controlling cowpea thrips. The traditional Chinese medicine ginseng and asparagus root achieved 92-95% effectiveness in controlling cowpea thrips.

[0078] Experimental Example 3

[0079] A cowpea planting base in Sanjiang Town, Meilan District, Haikou City, Hainan Province, conducted a comparative experiment on the control of cowpea thrips using chemical pesticides and formulations from 2024 to 2025, covering an area of ​​6 mu (approximately 0.4 hectares). The chemical pesticide experiment was compared with a comparative experiment using the interleaved method. There were three replicates, each covering an area of ​​1 mu (approximately 0.067 hectares). Chemical pesticides, including chlorantraniliprole, acetamiprid, and bifenthrin, were applied alternately starting after cowpea emergence. Formulations were sprayed every 5-7 days, with 200-300 ml per mu diluted in 20-30 liters of water for foliar spraying each time. The total dosage per mu was 8-9 liters.

[0080] Experimental results: Chemical pesticides were 63-70% effective in controlling cowpea thrips. The traditional Chinese medicine ginseng and asparagus root achieved 91-96% effectiveness in controlling cowpea thrips.

[0081] Based on the above, it can be concluded that

[0082] 1. This invention realizes the cross-disciplinary application of the theory of compatibility in traditional Chinese medicine: Sophora flavescens and Veratrum nigrum are the principal drugs; Melia toosendan and Stemona japonica are the assistant drugs; Gleditsia sinensis, Glycyrrhiza uralensis and Mentha haplocalyx are the adjuvant drugs; based on the compatibility principle of "principal, assistant, adjuvant and adjuvant" in traditional Chinese medicine, a compound Chinese medicine preparation of Sophora flavescens and Veratrum nigrum with multiple targets, multiple functions and synergistic effects is designed.

[0083] 2. This invention discloses for the first time and claims protection the application of a traditional Chinese medicine compound preparation with a specific weight ratio of "Sophora flavescens-Veratrum nigrum-Melia toosendan-Stemona japonica" as the core and "Gleditsia sinensis-Glycyrrhiza uralensis-Mentha haplocalyx" as the auxiliary ingredients in the green control of cowpea thrips. In particular, the addition of Gleditsia sinensis to the compound enhances the insecticidal effects of the principal ingredients, Sophora flavescens-Veratrum nigrum, and the assistant ingredients, Melia toosendan-Stemona japonica. This specific combination and its synergistic effect have not been reported in the prior art.

[0084] 3. The traditional Chinese medicine theories used in human medicine are systematically and purposefully applied to the green control of cowpea thrips pests, realizing the intelligent transfer and cross-border innovation from "Chinese medicine human medicine" to "Chinese medicine agricultural plant-derived medicine".

[0085] 4. This invention not only provides formulations, but also includes a complete green control technology procedure based on traditional Chinese medicine principles of "prevention before disease" and "treating before disease occurs" from the seedling stage, vine stage, flowering and pod-setting stage of cowpeas. This forms a complete and standardized green control technology system for cowpea pests, rather than remaining a research field confined to the laboratory.

[0086] 5. Raw materials are readily available, and the preparation process is mature: All the medicinal herbs used for the green control of cowpea thrips are common medicinal herbs with large-scale planting and market supply, ensuring a stable source. The preparation process of the Ginseng and Reed Compound Traditional Chinese Medicine Preparation adopts advanced water extraction and microbial fermentation technology, which is energy-saving, zero-emission, and environmentally friendly. It operates under normal temperature and pressure conditions, making it easy to achieve standardized and large-scale production.

[0087] 6. Safe to use and environmentally friendly: Non-toxic to humans and animals: The ingredients of the Shenlu compound preparation are all natural medicinal plants, which are safe for humans and animals at the recommended dosage; No harmful residues: It is easily degraded in the environment and will not form persistent residues in cowpeas and soil. It not only ensures the quality and safety of cowpeas, but also significantly improves the nutritional quality and flavor of cowpeas; Protects natural enemies: It has minimal impact on earthworms, frogs, beneficial insects and microorganisms in the soil, which is conducive to maintaining and protecting the ecological balance of farmland.

[0088] 7. Significant control effect and high economic benefits: Field trials have proven that this formulation achieves a preventive effect of over 90% and a curative effect of over 85% against cowpea thrips, surpassing the effectiveness of conventional chemical pesticides. Because it enhances cowpea's induced resistance and defense against thrips, its application reduces the occurrence of other pests (aphids, spider mites, bean pod borers), strengthens cowpea's resistance, and demonstrates a significant yield-increasing effect. It meets the production standards for organic and green cowpea vegetables, significantly enhancing the market value and competitiveness of cowpea vegetables.

[0089] 8. Delaying thrips resistance: The multi-target mechanism of action makes it difficult for cowpea thrips to develop adaptive mutations in a short period of time, which can effectively extend the service life of this product and has long-term application value.

[0090] In summary, this invention creatively develops a highly efficient, safe, and environmentally friendly compound insecticide using traditional Chinese medicine by deeply integrating the essence of traditional Chinese medicine theory and principles with modern plant protection science. It not only possesses outstanding creativity and novelty in its formulation and mechanism of action, but also demonstrates strong practicality in terms of raw materials, production, use, and efficacy. It provides a comprehensive technical solution with significant market potential and technological value for the sustainable management of cowpea thrips and the development of green ecological agriculture.

[0091] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A traditional Chinese medicine compound preparation for the green control of cowpea thrips, characterized in that, The traditional Chinese medicine compound preparation comprises the following raw materials by weight: Sophora flavescens, 20-30 parts; Veratrum nigrum, 20-25 parts; Sichuan pepper, 10-15 parts; 10-15 doses of Stemona japonica; Soapberry, 5-10 parts; Peppermint, 5-10 parts; Licorice, 5-10 parts.

2. A traditional Chinese medicine compound preparation for the green control of cowpea thrips according to claim 1, characterized in that, The preparation method of the traditional Chinese medicine compound preparation includes the following steps: S1. Raw material preparation: Prepare Sophora flavescens, Veratrum nigrum, Melia toosendan, Stemona japonica, Gleditsia sinensis, Mentha haplocalyx, and Glycyrrhiza uralensis according to the above weight proportions; S2. Raw material pretreatment: Remove impurities from the prepared raw materials, wash them, cut Sophora flavescens, Veratrum nigrum, Melia toosendan and Stemona japonica into small sections, crush Gleditsia sinensis, and keep the peppermint and licorice root intact for later use. S3. Crushing: Crush the pre-treated raw materials into fine powder using a high-speed crusher to ensure that the material is uniform and delicate. S4. Mixing and sieving: Put the pulverized fine powder into the mixing equipment and stir at low speed to ensure that the powder is fully and evenly mixed and to avoid clumping; sieve the evenly mixed powder through a sieve to remove large particles and ensure that the fineness of the preparation is consistent. S5. Cooking: Place the mixed and sieved ingredients in a stainless steel saucepan, add purified water and bring to a boil over high heat, then reduce to a simmer and stir occasionally. S6. Cooling: Let the prepared liquid stand and cool, avoiding direct sunlight or violent shaking during the process to maintain the stability of the active ingredients. S7. Adding microbial agents to the cooled stock solution and place it in a sterile fermentation tank for fermentation. S8. Negative pressure filtration: Transfer the fermented liquid to a sterile negative pressure filtration device, apply negative pressure, and filter to remove residual bacteria, particulate impurities, and large molecular precipitates. After filtration, collect the clear and transparent filtrate to obtain the original Chinese medicine solution; S9. Preparation: Dilute the obtained Chinese herbal extract with purified water and mix at low speed using a stirrer to ensure uniformity and no sedimentation; at the same time, adjust the pH value to keep the active ingredients stable; after preparation, a clear liquid preparation that can be directly used for spray application is obtained and stored in a light-proof container for later use.

3. A traditional Chinese medicine compound preparation for the green control of cowpea thrips according to claim 1, characterized in that, The fineness of the powder in the pulverizing step is 80-100 mesh.

4. A traditional Chinese medicine compound preparation for the green control of cowpea thrips according to claim 1, characterized in that, In the mixing and sieving step, the low-speed stirring speed is 200-300 rpm, and the stirring time is 15-20 minutes.

5. A traditional Chinese medicine compound preparation for the green control of cowpea thrips according to claim 1, characterized in that, In the cooking step, the ratio of fine powder to water is 1:10, and the cooking time is 2 to 3 hours.

6. A traditional Chinese medicine compound preparation for the green control of cowpea thrips according to claim 1, characterized in that, The cooling temperature during the cooling step is 25–35°C.

7. A traditional Chinese medicine compound preparation for the green control of cowpea thrips according to claim 1, characterized in that, The fermentation time in the above-mentioned fermentation step is 5 to 7 days, and the microbial agent is selected from beneficial microbial strains such as lactobacillus or brewer's yeast.

8. A traditional Chinese medicine compound preparation for the green control of cowpea thrips according to claim 1, characterized in that, The negative pressure in the negative pressure filtration step is 0.05 to 0.1 MPa; the filtration is carried out using a filter membrane with a pore size of 0.45 micrometers.

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