262 results about "Metarhizium anisopliae" patented technology

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, is used for improving soil / land and especially can be used for effectively improving soil polluted by heavy metals.

The invention discloses Metarhizium anisopliae and application of Metarhizium anisopliae. The Metarhizium anisopliae provided by the invention is Metarhizium anisopliae JF-6213 with CGMCC No. 5952 registered in China General Microbiological Culture Collection Center. The strain (Metarhizium anisopliae) has good production traits and strong spore production capacity, strong insecticidal activity to sugarcane moth borer, good secondary infection to sugarcane moth borer, has 7.44 days LT50 (lethal time of 50%) to 3-4 years sugarcane moth borer; the strain is used for preparing insect killing fungus preparation which can effectively control population quantity of sugarcane moth borer; moreover, Metarhizium anisopliae JF-6213 has strong pathogenicity to brontispa longissima, asiatic migratory locust, leechee stinkbug and Dysmicoccus neobrevipes Beardsley, strong secondary infection capability and high stability and is quite applicable to tropical and subtropical areas.

The invention relates to a metarhiziumanisopliae compound insecticide composition and use thereof. The metarhiziumanisopliae compound insecticide composition is characterized in that: the active ingredients of the composition include metarhiziumanisopliae and a new neonicotinoid insecticide; the density of the metarhiziumanisopliae in the composition is 1 to 10 billion spores per gram; and the new neonicotinoid insecticide content of the composition is 20 mass percent; and the rest content of the composite are pesticide auxiliary ingredients. The composition has an obvious synergistic effect on grubs and is suitable for preventing and controlling plant pests, particularly grubs. The composition can be used on various crops such as lawns, peanut, sweet potatoes and vegetables or in various places and has the advantages of stable insecticide effect, high quick-acting performance, long efficacy duration, safety and environmental protection.

The invention belongs to microbial fermentation, and particularly relates to a preparation method of a probiotic inoculum for preventing replant disease of greenhouse cucumbers. The preparation method comprises a fermentation step: inoculating a probiotic inoculum producing strain into a sterile culture medium containing a carbon source, a nitrogen source, inorganic salt and water; carrying out aerobic fermentation under the conditions that the temperature is 30-37 DEG C and the pH value is 7.2-7.6 in the fermentation process, wherein the producing strain is a mixture of Bacillus subtilis, Bacillus licheniformis, Trichosporon behrendii, Paecilomyces lilacinus, Streptomyces microflavus, Bacillus polymyxa and Metarhizium anisopliae of the same mass; and adsorbing the final fermentation product, and immobilizing on grass carbon to obtain the probiotic inoculum. The preparation method solves the defects of replant disease, root rot, blight, nematode disease and the like in the existing greenhouse vegetable-cucumber planting technology, has the advantages of low production cost, short fermentation period and high flora density, and can effectively prevent seedling death, seedling yellowing, blight, mosaic disease, root rot and other ecological symptoms of soil. Meanwhile, the preparation method can promote the growth of roots, loosen the soil, strengthen the seedlings and degrade the residual pesticides and fertilizers, and has an effect of obviously restoring the soil ecosystem. By using the preparation method, the yield of greenhouse cucumbers can be increased by 15-20% in one season.

The invention discloses a pathogenic fungus for biological control of cockroaches. The classification name of the bacterial strain is metarhizium anisopliae which has been preserved in the China microbiological culture collection management committee general microbiological center with the serial number being EB0732 and the preservation number being CGMCC No.13170. The pathogenic fungus for biological control of cockroaches has the beneficial effects that (1) the pathogenic fungus is not prone to enabling insect pests to have resistance to chemicals; (2) the pathogenic fungus has strong selectivity for a host, and is safe for the environment; and (3) the pathogenic fungus has a strong lethal effect on male and female insect pests of different instars.

A biopesticidal composition for controlling insects (e.g., pecan weevils, the diaprepes root weevil, fall armyworm, fire ants), containing an agriculturally acceptable carrier and an effective insect (e.g., pecan weevils, the diaprepes root weevil, fall armyworm, fire ants) biopesticidal amount of a fungus selected from the group consisting of Beauveria bassiana having the identifying characteristics of Beauveria bassiana NRRL 30593, Metarhizium anisopliae having the identifying characteristics of Metarhizium anisopliae NRRL 30594, Beauveria bassiana having the identifying characteristics of Beauveria bassiana NRRL 30601, Beauveria bassiana having the identifying characteristics of Beauveria bassiana NRRL 30600, or mixtures thereof. Also, a method for controlling insects (e.g., pecan weevils, the diaprepes root weevil, fall armyworm, fire ants), involving applying an effective insect biopesticidal amount of the composition to the insects or to the plants, areas or substrates infested with the insects.

The invention relates to a preparation method and application of Beauveria bassiana, which especially has an effect on control of locust pests. The Beauveria bassiana (Bals. -Criv.)Vuill TRCC16001 provided by the invention has a registered number of CGMCC No.7997 in China General Microbiological Culture Collection Center. With a strong spore production ability, the strain has strong insecticidal activity on pygmy grasshoppers 3rd instar locust nymphae, and the lethal time of 50% LT50 is 3.04 days. The strain can be employed to effectively control the population quantity of pygmy grasshoppers. In addition, locust Metarhizium anisopliae also has very good prevention effect on other pests Dendrolimus punctatus, ostrinia nubilalis, grubs and the like, thus being worthy of popularization and application.

The invention relates to an application of a Metarhizium anisopliae CQMa421 strain in preparation of insecticide for preventing and controlling Striped flea beetle or plutella xylostella. The strain is capable of infecting Striped flea beetles and plutella xylostella with fatality rates being 60.9% and 70.8% respectively. The Metarhizium anisopliae CQMa421 strain with enhanced toxicity, which is prepared according to a method disclosed by the invention, has a fatality rate reaching 100% and achieves an outstanding prevention and control effect. The insecticide for preventing and controlling Striped flea beetles or plutella xylostella comprises dry conidial powder of Metarhizium anisopliae CQMA421 (44-60%), kieselguhr (30-40%), nano-aluminum trihydrate (0.5-1%), sodium carboxymethylcellulose (3-5%), tween-80 (3-5%) and OP-10 (3-5%). All the components in the Metarhizium anisopliae insecticide are matched effectively to take a synergistic effect. After being mixed with water, the Metarhizium anisopliae insecticide is applied in a spraying way to achieve a field prevention effect of 80% or above for rice planthopper. The Metarhizium anisopliae insecticide has excellent wetting dispersion characteristic, wherein the wetting time is less than 60s; and in addition, the bioactivity during long-term storage can be retained and the storage time is as long as 12 months.

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, can survive for a long term in a plurality of improved soil to play a role, is good in adaptation of improving soil / land and especially can be used for effectively improving a plurality of soil, such as secondary salinization land, desertification soil.

The invention discloses entomogenous fungi chafer metarrhizium anisopliae strain CQMa102 acidity trehalase and its manufacturing method. It adopts active chafer metarrhizium anisopliae strain CQMa102 spore to form enzyme in culture medium and purify it. The formed protease is glycoprotein. Its isoelectric point is 4.7. Molecular weight is 170kDa. Trehazolin is its specific inhibition. And its IC50 is 1.3*10-8M. The protein plays important role in trehalose competive in infection insect course. Meanwhile the purified trehalase can be applied to its content qualitative and quantitative detection in industry and other fields.

The invention provides metarhizium anisopliae MAYX130921 and an application thereof. The metarhizium anisopliae MAYX130921 is preserved on Dec 4th, 2014 in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.10139. The metarhizium anisopliae MAYX130921 is a bacterial strain initially separated from holotrichia parallela larvas, is easy to culture and can grow rapidly, sporulation quantity is high, and spore germination rate is high; meanwhile, conidiospores of the metarhizium anisopliae MAYX130921 have strong pathogenicity on the holotrichia parallela larvas, are environment-friendly and hardly produce drug resistance and can be widely applied to prevention and control of holotrichia parallela.

The invention relates to a biological pesticide composition, in particular to a fungus and plant extract pesticide composition. An insecticidal composition containing metarrhizium anisopliae and veratrine is characterized by mainly comprising the metarrhizium anisopliae and the veratrine, wherein the metarrhizium anisopliae and the veratrine are mixed according to the weight ratio being (1-20):(10-2). The metarrhizium anisopliae is metarrhizium anisopliae conidia, and the content is 5-40 billion spores / gram. The veratrine is a pure substance or a mixture containing veratrum total alkali. The insecticidal composition is environmentally friendly, and can expand the insecticidal spectrum of biological pesticides, be higher in insecticidal effect and longer in lasting period, and reduce the use frequency of pesticides.

The invention relates to the field of biological pesticides, and specifically relates to a metarrhizium anisopliae granular preparation and a preparation method thereof. The metarrhizium anisopliae granular preparation comprises the following components in percentage by weight: 1-5% of metarrhizium anisopliae conidial powder, 90-95% of carriers, 0.01-0.45% of a promoting agent, 0.01-0.45% of a protective agent, and 1-5% of a lubricant. According to the technical scheme of the invention, the addition of aids is reduced, thereby significantly reducing the processing and manufacturing costs of granules. Under the premise of not adding an adhesive, a disintegrating agent and a disintegrating aid, through using the self physical and chemical properties of the carriers and the adjustment of water addition, the granulating property and the disintegration property of the carrier are solved well. Granules are relatively easy to dry, thereby reducing the energy consumption of drying. Through the adjustment of water addition, heat generated by granulating can be greatly reduced, and the damages on metarrhizium anisopliae spores caused by the granulating process can be reduced. Through adding small amount of the promoting agent, the germination of metarhizium anisopliae spores can be significantly promoted; and the damages on spores caused by the granulating process can be significantly reduced, and the problems of thermal damages and mechanical damages in the granulating process are solved better.

The invention discloses maggot-bacterium bio-active fertilizer and a preparation method thereof. Raw materials comprise maggots, parasitic pathogenic microorganisms and organic additives. The maggots are fly larvas, are rich in proteins and are innocuous and unpoisonous. The parasitic pathogenic microorganisms comprise one or more of metarhizium anisopliae, beauveria bassiana and bacillus thuringiensis. The organic additives comprise one or two of livestock and poultry night soil and dead livestock and poultry treated at a high temperature. The maggot-bacterium bio-active fertilizer belongs to the field of crop fertilizer, is high-protein active biofertilizer, improves soil fertility, promotes plant growth, provides the living parasitic pathogenic microorganisms, and can kill larvas by infecting insect larvas thereby reducing insect attack. The maggot-bacterium bio-active fertilizer is suitable for organic agriculture, green agriculture and nuisance-free agriculture production. The preparation method has simple processes, can be operated easily, is conducive to popularization and can improve agriculture production benefits and peasant income.

The invention relates to a strain of Metarhizium anisopliae FM-03 and its application in prevention and control of mealybugs and belongs to the technical field of biological prevention and control. The Metarhizium anisopliae FM-03 has an accession number of CGMCC No. 13774. The growth performance of Metarhizium anisopliae is good, the sporulation rate is high, the germination rate of spores is high, and the lethal effect on mealybugs is good. The Metarhizium anisopliae FM-03 is safe to the natural enemy, has the potential to replace a part of the mealybug chemical pesticide, reduces a use amount of a chemical pesticide and is conducive to keep the ecological balance.

The invention belongs to the technical field of biology, and particularly relates to Metarhizium anisopliae IPPMHBC-009 and an application thereof. The preservation number of the Metarhizium anisopliae IPPMHBC-009 is CGMCC No.16000, and the Metarhizium anisopliae IPPMHBC-009 has good median lethal time and cadaver rate for oedaleus decorus asiaticus and dasyhippus barbipes.

The invention discloses an application of a metarhizium anisopliae CQMa421 strain in the preparation of a medicine for preventing and treating aphids. The medicine for preventing and treating the aphids is prepared from the following raw materials in percentage by weight: 35%-52% of metarhizium anisopliae CQMa421 xerospore powder, 40%-50% of kieselguhr, 1%-2% of nanometer aluminum oxide, 3%-5% of sodium carboxymethylcellulose, 2%-4% of OP-10 and 2%-4% of HEL-40. Black peach aphids and lipaphis erysimi kaltenbach can be infected by the metarhizium anisopliae CQMa421 strain; and the fatality rate on the black peach aphids and the lipaphis erysimi kaltenbach can respectively reach 71.5% and 70.8%. The fatality rate of the metarhizium anisopliae CQMa421 strain prepared by adopting a method for enhancing the toxicity can reach 100% and is improved by 30%; and the effect is very outstanding. Various components of a metarhizium anisopliae preparation effectively cooperate to reach the effect of synergistic interaction. The metarhizium anisopliae preparation disclosed by the invention is sprayed by blending in water; and the field control effect on the black peach aphids and the lipaphis erysimi kaltenbach reaches 80% or above. The preparation has good ultraviolet resistant characteristic and wetting dispersion characteristic; the biological activity in long-term storage is kept; and the storage time can reach 12 months.

The invention provides an o-methyltransferase with a wide range of substrates, and can be applied to oxygen methyl transfer in a biosynthesis process of an organic compound. An experiment proves the catalytic activity of the o-methyltransferase, and shows that the o-methyltransferase can be applied to a plurality of substrates. The invention further provides a synthesis method for a hydroquinone lactone compound. The hydroquinone lactone compound is obtained by transforming an o-methyltransferase gene and a hydroquinone lactone synthetic gene into yeast cells, carrying out fermentation cultivation and extracting a product.

Alates of the Formosan subterranean termite (FST), Coptotermes formosanus Shiraki, collected after swarming in 2002 died within 48 hrs, and the cadavers were visibly infected with a fungus. Fungi were picked from the cadavers, transferred to media and ultimately isolated to purity. A single fungal isolate, C4-B, taxonomically identified as Metarhizium anisopliae (Metschnikoff), was found to cause rapid mortality of FST alates. This is the first report of a biological control agent for termite alates. Strain C4-B was more lethal to both alates and workers when compared to M. anisopliae strain ESC1, marketed as the termite biocontrol agent BioBlast®. 100% of FST alates exposed to C4-B spores (106 spores / μl) were killed in 3 days. Field studies where C4-B spores were dispersed on grassy lawns resulted in 100% mortality of alates by day 5.

The invention discloses a pesticidal main agent by compounding destruxins of Metarrhizium anisopliae and decamethrin. The main agent is a mixture of the destruxins of Metarrhizium anisopliae and the decamethrin; and the mass ratio of the destruxins of Metarrhizium anisopliae to the decamethrin is 1-9:9-1. The pesticidal main agent selects the the destruxins of Metarrhizium anisopliae and the decamethrin for compounding, and determines the optimal mixing ratio of both, so as to obtain obvious synergy and effectively solve the drugfastness problem of pests such as diamond back moth and prodenia litura. The pesticidal main agent has good desinsection effect and strong practical application and promotion, and has important significance of preventing and controlling the pests such as the diamond back moth and the prodenia litura; and the pesticide is environment-friendly.

The invention discloses a method for culturing Metarhizium anisopliae, which comprises the following steps of: 1) performing liquid fermentation culture on the Metarhizium anisopliae by using a fluid nutrient medium, and marking all substances in a container as liquid fermentation products; and 2) inoculating the liquid fermentation products into a solid fermentation medium for solid fermentation culture to obtain the Metarhizium anisopliae. The method is a method for culturing the Metarhizium anisopliae by liquid-solid dual-phase fermentation, and combines liquid fermentation tank production with solid fermentation production. In liquid submerged fermentation, the medium adopted takes bagasse as a main carrier, three cheap and readily available agricultural products are taken as raw materials, and the cost is greatly reduced.

The invention provides a bag type solid cultivation process for Metarhizium anisopliae, comprising steps: inoculating Metarhizium anisopliae liquid cultivation seed at a ratio of 1 to (3-7) in closed cultivation bags filled with a sterilized and cooled solid medium, mixing uniformly, sealing, laying on a cultivation rack for culturing, culturing at 18-22 DEG C for 5-7 days until mycelia grow densely, scattering by rubbing before spore production, and introducing air; performing continuous cultivation at 24-26 DEG C for 5-7 days, scattering by rubbing, opening the bags and drying. The solid cultivation medium is one or a mixture selected from wheat bran, chaff and rice, and the closed cultivation bag is a closed fungal insecticide solid fermented cultivation bag. The process is suitable for the solid cultivation of Metarhizium anisopliae of different scales, has the advantages of being easy and convenient to operate, low in pollution rate, high in spore yield, and the like.

The invention provides a biological enzyme soil improver and a preparation method thereof. The invention provides the biological enzyme soil improver capable of adjusting the alkalinity and acidity of soil and the preparation method of the biological enzyme soil improver. The biological enzyme soil improver is prepared from the following components in parts by mass: 25 to 35 parts of lactic acid bacteria, 8 to 12 parts of nitrogen-fixing bacteria, 8 to 12 parts of actinomyces, 3 to 8 parts of silicate bacteria, 6 to 10 parts of bacillus subtilis, 2 to 5 parts of beauveria bassiana, 2 to 5 parts of metarhizium anisopliae, 3 to 8 parts of paenibacillus polymyxa, 3 to 8 parts of trichoderma viride, 8 to 12 parts of saccharomycetes, 3 to 8 parts of photosynthetic bacteria and 0.7 to 1.1 parts of a biological enzyme, wherein the concentration of effective living bacteria is 10billion / mL to 20billion / mL. According to the biological enzyme soil conditioner provided by the invention, various beneficial microorganism florae are scientifically proportioned; meanwhile, a novel amino acid state chelating agent is utilized and the growth and the proliferation of complex flora are accelerated; meanwhile, the chelating capability on microelements is enhanced in the presence of ultrasonic waves, the utilization rate is greatly improved and the yield increasing effect is very remarkable.

The invention discloses a pesticide compounding beauveria bassiana fungus and destruxin. The pesticide is prepared by mixing the beauveria bassiana fungus, the destruxin and solvent, the concentration of live conidia of the beauveria bassiana fungus after mixing ranges from 10<7> to 10<11> conidia per liter, and the pesticidal effect is better when the concentration of the destruxin ranges from 10 mg / L to 100 mg / L. The preparation method for the pesticide comprises the following steps: preparing conidial suspension of the beauveria bassiana fungus, preparing destruxin stock solution, mixing the conidial suspension and the destruxin stock solution, and the like. The invention selects the beauveria bassiana fungus to be used with the destruxin in a mixing way for synergic action, and the pesticide has the completely new function mechanism different form the prior pesticide, has an excellent pesticidal effect particularly in poisoning and killing bemisia tabaci gennadius with serious harm, and meanwhile is environment-friendly.

The invention discloses a metarhizium strain pathogenic to Laos false blister beetles and application of the metarhizium strain. The metarhizium strain is named as metarhizium anisopliae [FJMa200902, which is stored in China general microbiological culture collection center (CGMCC)], and the collection number is CGMCC No.10642. The strain grows fast and has strong spore formation capability, good production traits and high pathogenicity to larvae of the Laos false blister beetles; after the strain is inoculated by a spore suspension liquid of 107 spores / mL, LT50 (lethal time of 50%) of the strain to the larvae of the Laos false blister beetles is 3.6 days, and infection rate reaches 100%. A fungus preparation made from the strain has good prevention and treatment effects on the Laos false blister beetles, population density of the larvae of the Laos false blister beetles can be reduced effectively, and the metarhizium strain is a biocontrol strain which has application value for prevention and treatment of the Laos false blister beetles.

The invention discloses a metarhizium anisopliae strain with strong pathogenicity to curculio nucum and applications of the metarhizium anisopliae strain. The metarhizium anisopliae strain provided by the invention is Metarhizium anisopliae CoCn01, is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 12, 2016 through a patent preservation method, and is assigned with the accession number of CGMCC No.12167. The strain is high in growth speed and strong in spore production capacity, and is separated from the adult corpse of curculio nucum suffering from natural death for the first time, a culture method is simple, the propagation speed is high, the metarhizium anisopliae strain has relatively strong pathogenicity to both the adult and larva curculio nucum, is harmless to human beings and the livestock, and is free of pollution to the environment, the pests have difficulty in generating drug resistance, and thus the metarhizium anisopliae strain can be widely used for preventing and treating curculio nucum, and belongs to a biological control strain which has an ultrahigh application value in the prevention and treatment for curculio nucum.

The invention belongs to the technical field of biology, and particularly relates to metarhizium anisopliae IPPMHBHC-7 and application thereof. The preservation number of the metarhizium anisopliae IPPMHBHC-7 is CGMCC No.15999, and the metarhizium anisopliae IPPMHBHC-7 has the good median lethal time and insect dead rate for Oedaleus decorus asiaticus and Dasyhippus barbipes.

A biopesticide for herbaceous plants includes the following components, by weight: 0.8-1.2 parts of bacillus sphaericus, 0.6-0.9 parts of bacillus thuringiensis, 2-6 parts of metarhizium anisopliae, 4-7 parts of paecilomyces lilacinus, 3-7 parts of avermectin, 4-8 parts of ivermectin, 5-9 parts of amino oligosaccharin, 4-8 parts of sophocarpidine, 3-7 parts of veratrine, 6-11 parts of osthole, 0.9-1.6 parts of cis-9-tricosene, 57-65 parts of a synergist, and 12-19 parts of 8% ethanol, and preferably includes, by weight, 1 part of the bacillus sphaericus, 0.7 parts of the bacillus thuringiensis, 4 parts of the metarhizium anisopliae, 6 parts of the paecilomyces lilacinus, 5 parts of the avermectin, 6 parts of the ivermectin, 7 parts of the amino oligosaccharin, 6 parts of the sophocarpidine, 5 parts of the veratrine, 8 parts of the osthole, 1.2 parts of the cis-9-tricosene, 61 parts of the synergist, and 16 parts of the 8% ethanol. The biopesticide is excellent in effects, and is free of toxic and side effects, pesticide residue and ecologic and environmental pollution.

The invention relates to application of a metarhizium anisopliae CQMa421 strain in preparation of a medicament for preventing and treating rice planthoppers. The medicament for preventing and treating the rice planthoppers is prepared from the following components by weight percent: 35-52% of metarhizium anisopliae CQMa421 dry spore powder, 40-50% of diatomite, 1-2% of nanometer silicon, 3-5% of sodium carboxymethylcellulose, 2-4% of O-30 and 2-4% of HEL-40. The rice planthoppers can be infected by the metarhizium anisopliae CQMa421 strain, and the fatality rate can reach 32.6 percent. The metarhizium anisopliae CQMa421 strain prepared by a toxicity increasing method has the fatality rate of 98.7-100%, LT50 is shortened by 57.4-61.4%, and the effect is very prominent. The components of the metarhizium anisopliae preparation are effectively matched to play a synergistic interaction role, and filed efficacy on the rice planthoppers can reach 80% above by spraying the metarhizium anisopliae preparation added with water. The preparation has good ultraviolet resistance and dispersing and wetting properties, the biological activity in long-term storage is kept, and the storage time is up to 12 months.

The invention discloses a biological organic fertilizer. The biological organic fertilizer is mainly prepared by mixing and fermenting the following raw materials in parts by weight: 50-60 parts of rapeseed meal, 10-20 parts of plant straws, 8-12 parts of garlic residues, 5-7 parts of bagasse, 8-12 parts of oyster shell powder, 2-6 parts of a calcium magnesium phosphate fertilizer, 6-10 parts of amino acid liquid, 20-30 parts of sewage, 1-2 parts of wood vinegar, 0.5-1.5 parts of a decomposed microbial inoculum and 1-3 parts of a composite functional microbial inoculum, wherein the composite functional microbial inoculum is a mixed microbial inoculum of bacillus amyloliquefaciens, bacillus mucilaginosus, bacillus megatherium and metarhizium anisopliae. The various microbial inoculums contained in the decomposed microbial inoculum added in the invention are not antagonistic to one another, and can have a synergistic effect in a fermentation process, so that growth of bacterial strains and production of metabolites are promoted, decomposition of fermentation raw materials is accelerated, and the fermentation period is shortened.