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Bag type solid cultivation process for Metarhizium anisopliae

A technology of solid culture and solid medium, which is applied to the field of solid fermentation of insect-borne fungi, can solve the problems of high production cost, high price and high pollution rate, and achieves the effects of flexible and controllable culture scale, easy acquisition and low pollution rate.

Inactive Publication Date: 2017-12-08
GUANGDONG ACAD OF FORESTRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liquid culture can adopt liquid fermenter or shaker culture according to the culture scale. The culture technology is relatively mature, while the solid-state culture has been mainly plate culture for many years, because of the shortcomings of high pollution rate, low spore production and low live spore rate. Product quality, the large-scale solid fermentation equipment developed in recent years is also not widely used because of its high price, large floor area, high energy consumption, and high production cost.

Method used

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  • Bag type solid cultivation process for Metarhizium anisopliae
  • Bag type solid cultivation process for Metarhizium anisopliae
  • Bag type solid cultivation process for Metarhizium anisopliae

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1 scarab metarhizium anisopliae (Metarhizium anisopliae) bag culture process

[0030] Cultivation of Metarhizium seed solution in shake flask: sticky rice flour 420g, white sugar 630g, K 2 HPO 4 21g, MgSO 4 10.5g of peptone, 105g of peptone, added mineral water to make 21L culture medium, divided into 30 1000mL Erlenmeyer flasks, 700mL per bottle, plugged with silica gel caps, wrapped with kraft paper, placed in a 100L vertical sterilizer, and sterilized at 121°C. Bacteria for 30 minutes, take it out and cool it down and put it in a clean bench, insert 1 / 3 of a bottle of Metarhizium anisopliae seeds on the slant, at 25-26°C, 200rpm reciprocating rotation for 72 hours, obtain liquid seeds, and set aside.

[0031] Solid culture process: use rice as a solid medium, add water according to 10% of the weight, mix well until the surface of the rice is wet, pack it in a fresh-keeping bag according to 2kg per bag, place it for 20-30min until the rice absorbs the sur...

Embodiment 2

[0035] Embodiment 2 Metarhizium flavoviride (Metarhizium flavoviride) bag culture process

[0036] Cultivate liquid seeds in fermenter: take 1000mL shake bottle and pack 400mL PPD medium, inoculate slant seeds of Metarhizium anisopliae after sterilization, culture at 25-26℃, 200rpm reciprocating rotation for 72h, and connect it to the medium of fermenter seed tank as seed liquid (10 liters canned 7L medium: sticky rice flour 2%, white sugar 3%, K 2 HPO 4 0.1%, MgSO 4 0.05%, peptone 0.5%), cultivated at a temperature of 25-26°C for 3 days, transferred to a large fermenter (100L medium 70L), and cultivated for 72 hours as seeds inoculated on solid medium.

[0037] Solid culture process: use rice as a solid medium, add water at 8% by weight, mix well until the surface of the rice is moist, pack 1.9kg per bag into a fresh-keeping bag, and place it for 20 minutes until the rice absorbs the surface moisture, knead and disperse , sealed, sterilized at 121°C for 30 minutes, poured ...

Embodiment 3

[0040] Example 3 Guizhou Metarhizium guizhouense (Metarhizium guizhouense) bag culture process

[0041] The liquid seed culture adopts the reciprocating rotation culture of the shake flask, and the method is the same as the seed liquid culture of the Metarhizium anisopliae of Example 1.

[0042] Solid culture process: use rice as a solid medium, add water and mix well until the surface of the rice is moist, drain and pack in fresh-keeping bags, 2Kg per bag, sterilize at 121°C for 30 minutes, and pour it into a sterile sealed container after cooling Insert 600mL of liquid seeds into the solid culture bag, knead the agglomerated rice, mix well, seal it, spread it on the culture rack with the ventilated side up, and cultivate it at 20-22°C for 4 days, knead the agglomerated rice The rice is ventilated with air, laid flat on a culture rack, and cultured at a temperature of 25-26°C for 6 days. After the spore production is complete, the bag is opened, dehumidified and dried, and th...

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Abstract

The invention provides a bag type solid cultivation process for Metarhizium anisopliae, comprising steps: inoculating Metarhizium anisopliae liquid cultivation seed at a ratio of 1 to (3-7) in closed cultivation bags filled with a sterilized and cooled solid medium, mixing uniformly, sealing, laying on a cultivation rack for culturing, culturing at 18-22 DEG C for 5-7 days until mycelia grow densely, scattering by rubbing before spore production, and introducing air; performing continuous cultivation at 24-26 DEG C for 5-7 days, scattering by rubbing, opening the bags and drying. The solid cultivation medium is one or a mixture selected from wheat bran, chaff and rice, and the closed cultivation bag is a closed fungal insecticide solid fermented cultivation bag. The process is suitable for the solid cultivation of Metarhizium anisopliae of different scales, has the advantages of being easy and convenient to operate, low in pollution rate, high in spore yield, and the like.

Description

technical field [0001] The invention relates to the field of solid fermentation of entomogenic fungi, and mainly relates to a bag-type solid fermentation culture process flow of Metarhizium anisopliae. Background technique [0002] The application of Metarhizium anisopliae in the control of subterranean pests created the history of biological control, and the use of entomopathogens to carry out pest control has become an important research field and control strategy for biological control. Entomogenic fungi have no residue, no pollution to the environment, and are specific to target pests. At present, dozens of entomogenic fungi have been used in the prevention and control of agricultural, forestry, animal husbandry and health pests, and have been used in biological control. Take a place. [0003] In recent years, more than 300 kinds of pests have been studied and applied to control pests, and about 100 kinds of pests have been tested in the field. It is one of the main fun...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 徐金柱秦长生赵丹阳邱华龙杨华揭育泽
Owner GUANGDONG ACAD OF FORESTRY
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