20 results about "Hyphal growth" patented technology

The invention belongs to the technical field of biology, and particularly relates to application of serine in relieving heat stress of pleurotus ostreatus and a method. Under the heat stress condition, exogenous serine supply promotes pleurotus ostreatus hypha to recover growth, and the activity of antioxidant enzyme, the yield of sporocarp and the yield of nutrient substances are improved; when the activity of the PHGDH enzyme is inhibited, the serine synthesis is reduced; the hypha growth can be promoted by overexpressing the phgdh, and the enzymatic activity of the PHGDH can be improved; when acetyltransferases HAT, NAT1, NAT2 and NAT3 act on a K394 site of the PHGDH, the enzyme activity of the PHGDH can be improved, endogenous serine metabolism is activated to respond to heat stress, it is indicated that PHGDH-mediated serine metabolism plays an important role in corresponding heat stress of the pleurotus ostreatus, and the invention discloses a mechanism for improving heat resistance of the pleurotus ostreatus through the PHGDH-mediated serine metabolism. And a foundation can be laid for breeding high-temperature-resistant pleurotus ostreatus dominant strains.

The invention relates to the technical field of hypha image processing, and discloses an image super-resolution reconstruction method for a hypha topological structure. The method comprises the following steps: acquiring a low-resolution topological image of a hypha sample and extracting an initial resolution matrix; performing topological structure correction on the initial matrix based on a preset hypha structure feature library to generate a first correction resolution matrix; and dynamically enhancing the data through a hypha growth simulator, and outputting a target resolution matrix. Meanwhile, an actual topological image is collected, an actual resolution matrix is extracted, and when the structural difference degree of the two exceeds a threshold value, a first deviation matrix is generated and the target matrix is iteratively adjusted to obtain a first adjustment matrix; and taking the adjustment matrix as a new target matrix, repeating the dynamic enhancement step until the structural difference degree is smaller than a threshold value, and realizing high-precision hypha topological image reconstruction. According to the method, the accuracy of hypha topological image super-resolution reconstruction is improved through feature library correction, dynamic enhancement and iterative adjustment.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The invention belongs to the technical field of microorganisms, and particularly relates to application of an oyster mushroom small heat shock protein (PoHSP20-6) gene in regulation and control of hypha heat resistance and a method for constructing an overexpression PoHSP20-6 gene oyster mushroom strain, and the nucleotide sequence of the PoHSP20-6 gene is shown as SEQ ID NO: 1. According to the invention, through a PoHSP20-6 overexpression vector, a PoHSP20-6 gene overexpression oyster mushroom strain is constructed and obtained. And it is proved that the hypha growth recovery capability of the overexpressed oyster mushroom strain under 40 DEG C high temperature stress is significantly improved, and the high temperature tolerance is significantly enhanced. The PoHSP20-6 gene and the oyster mushroom strain overexpressing the PoHSP20-6 gene provide key gene resources and technical support for fungus stress resistance breeding, can effectively solve the problem of poor hypha growth caused by high temperature stress in edible fungus cultivation, and have important application value in the edible fungus industry.

The invention belongs to the technical field of microorganisms, and discloses a pleurotus purpureus strain 162 and a cultivation method thereof. The preservation number of the strain 162 is CGMCC (China General Microbiological Culture Collection Center) No. 42041. The cultivation method comprises the following steps: 1) inoculating a pleurotus purpureus strain 162 on a mother strain culture medium to obtain a strain 162 mother strain; 2) inoculating the strain 162 mother strain to a stock culture medium to obtain a strain 162 stock; 3) inoculating the strain 162 stock seed to a cultivar culture medium to obtain a strain 162 cultivar; and 4) cultivating the cultivar of the strain 162 to obtain the sporocarp of the strain 162. The strain 162 is fast in hypha growth, short in after-ripening period, fast in fruiting, white in pileus, strong in adaptive capacity and short in harvesting period; the biological efficiency is high; the feed is high in nutritional value and has strong disease resistance to bacterial brown spot.

The invention provides a ganoderma strain SM07 and application thereof. The preservation number of the ganoderma strain (Ganoderma sp.) SM07 is CCTCC (China Center for Type Culture Collection) NO: M 20251816. The bacterial strain is developed in aerial hypha, rapid in hypha growth and high in hypha kinking property, straw can be used as a raw material to produce the biomass lining, and the biomass lining package is pure white and smooth in appearance, light and attractive in texture, has the loose and porous structural characteristic, is excellent in air permeability, can provide good buffering protection and can be used for preparing the biomass lining. And the quality stability in the tobacco leaf storage process is guaranteed. Besides, the environment-friendly characteristic is outstanding, the smoke biomass lining can be degraded by microorganisms in soil and converted into soil organic matter after being used, resource cyclic utilization of agricultural by-products is achieved, traditional plastic foam can be replaced, the problem of white pollution is avoided, and wide market prospects are shown in the field of green packaging.

The invention provides a new tremella strain variety HT108, which is formed by space radiation induced variation breeding by taking'ancient tremella Tr21 'as an original strain. The Tremella fuciformis Tr21 has the characteristics of high mycelial growth speed, high polysaccharide content in spores and the like, the sporocarp is yellow, the flower shape is round and compact, the tremella slices are thick and are internally rolled, the yield is high, the active ingredient content is high, particularly the total polyphenol content and the total flavone content are respectively increased by more than 30% compared with those of the contrast Tremella fuciformis Tr21, and the Tremella fuciformis Tr21 has good production and application values.

This invention discloses a non-destructive sampling method for studying the interaction between AM fungi and mycelial bacteria. Step 1: Sterilized culture medium is filled into the root chamber and the mycelial growth chamber, and the outer end of the mycelial growth chamber is sealed; the inner end of the mycelial growth chamber is connected to the root chamber. Step 2: Corn seeds are planted in the culture medium in the root chamber, and AM fungal spores are added to the root chamber while simultaneously cultivating them using nutrient solution and water. Step 3: Collection is performed, consisting of collecting pure mycelia and collecting fresh soil affected by pure AM fungal mycelia. This invention uses non-destructive sampling under pot conditions to collect pure, uncontaminated AM fungal mycelia and mycelial soil affected by AM fungi for scientific research on mycelial interactions.

The invention belongs to the field of marine natural product chemistry, and particularly relates to a marine fungus-derived alkaloid derivative and application thereof in prevention and treatment of pepper phytophthora blight. The marine fungus crude extract disclosed by the invention shows very strong antifungal activity on phytophthora capsici under the concentration of 300 mu g / mL. Seven monomeric compounds are separated and identified, NEA shows strong antifungal activity to phytophthora capsici and is stronger than positive control azoxystrobin, the NEA effectively inhibits hypha growth, sporangium formation and zoospore movement of phytophthora capsici hyphae, and meanwhile, the protective ability of pepper plants is enhanced; nEA causes obvious morphological change of phytophthora capsici protonema, so that cell membrane integrity damage, vacuole abnormal expansion, vacuole rupture, serious plasm-wall separation and disappearance of most organelles are caused. In addition, the content of MDA in the phytophthora capsici mycelium induced by the NEA is increased, the activity of antioxidant enzyme is reduced, and the level of soluble protein is reduced.

This invention relates to the field of pharmaceutical technology, specifically to the application of dehydronuciolin and fluconazole in the preparation of antifungal drugs. The in vitro synergistic effect of the combination of dehydronuciolin and fluconazole was determined using a checkerboard assay, showing a fractional inhibitory concentration (FIC) ≤0.5, confirming a strong synergistic effect. The in vivo efficacy of this drug combination was verified using a large wax moth infection model. Phenotypic experiments explored the synergistic mechanism, showing that the combination of dehydronuciolin and fluconazole significantly inhibited the hyphal growth, biofilm formation, and adhesion to host cells of drug-resistant Candida albicans. Transcriptome sequencing analysis confirmed these findings, showing that treatment with the combination of dehydronuciolin and fluconazole downregulated hyphae-specific genes, GPI-anchored cell wall proteins, and the ergosterol biosynthesis pathway, collectively weakening the virulence, surface integrity, and host recognition ability of Candida albicans, thereby reversing its drug resistance.

The present application belongs to the field of pharmaceutical chemistry, and particularly relates to Hypogin derivative and application thereof. The present application firstly simplifies the structure of Hypogin endogenous peptide, finds an active fragment, and designs and synthesizes Hypogin derivatives of series I. The Hypogin derivatives with better activity in series I are combined with cinnamic acid to design and synthesize compounds of series II. Research finds that the compound II-1 can better inhibit the hypha growth of Sclerotium rolfsii, and it is found that I-12 affects the signal pathway of PI3K of Sclerotium rolfsii, and the potential target is GPCR, RTK and PI3K of Sclerotium rolfsii. The cytotoxicity experiment shows that II-1 has low hepatocyte toxicity of human LO2, and conforms to the development direction of green bactericides. The Hypogin derivative has bacteriostatic activity, can be used as a basis for development of small peptide bacteriostatic agent, and has good application prospect.

The present invention relates to novel applications of thiazolinone as a fungal and oomycete chitin synthase inhibitor and its sterilization application. In vitro enzyme activity inhibition tests and sterilization tests demonstrate that thiazolinone can significantly inhibit the activity of fungal and oomycete chitin synthases, inhibit fungal spore production and hyphal growth, and inhibit the production and infection toxicity of oomycete sporangia. The invention can be used as a sterilant for the prevention and treatment of fungal and oomycete diseases.

The present disclosure relates to methods and compositions for genetically engineering microorganisms for improved control of pathogens such as bacteria and / or fungi, which can be applied in various fields such as medicine and agriculture. The genetically engineered microorganisms may comprise deletions or knockout at one or more genomic loci that confer the ability to reduce mycelial growth of the fungi, reduce sporogenesis of the fungi, and / or other benefits.

The invention discloses an application of an antibacterial peptide in preparation of a medicine for preventing and treating rice blast. The antibacterial peptide is 6-D-arginine. Research finds that 6-D-arginine can be used as a medicament for preventing and treating rice blast. The 6-D-arginine has an obvious inhibition effect on the formation of magnaporthe oryzae appressorium and the growth of mycelia. Therefore, the 6-D-arginine can be used for improving the resistance of the rice to the rice blast.

The invention discloses a hymenopellis raphanipes mutant strain M1 obtained through artificial mutagenesis, the hypha growth rate of the hymenopellis raphanipes mutant strain M1 is larger than or equal to 5.20 mm / d, the biological efficiency reaches 53.6%, the hymenopellis raphanipes mutant strain M1 has strong resistance to trichoderma harzianum, the proportion of first-grade mushrooms is larger than or equal to 40.6%, and the hymenopellis raphanipes mutant strain M1 is good in hereditary stability. The invention also provides a breeding method of the strain, a special liquid fermentation culture medium and an industrial cultivation process in an intelligent mushroom square cabin. The method solves the problems that traditional cultivation is long in period, high in pollution rate, difficult to factorize and the like, and a complete solution is provided for annual efficient production of oudemansiella oosporium.

The invention discloses a hypha collection device and a field hypha collection method for in-situ collection of AM (arbuscular mycorrhizal) fungi and inter-hypha bacterial communities thereof. The frame comprises a frame set, a back outer film, a front inner film, a front outer film and a partition plate, and the back outer film, the partition plate, the front inner film and the front outer film are installed in the frame set from the back face to the front face. The distance between the back outer film and the partition plate is 1-2 mm, the distance between the partition plate and the front inner film is 1-2 mm, and the distance between the front inner film and the front outer film is 1-2 mm. The length of the short side of the cuboid plate-shaped frame group is at least eight times of the thickness, so that an effective length is formed on the opposite angles to collect enough hyphae. The hypha collecting device is in a plate shape, so that the thickness is small, the hypha collecting device can be placed between any two crops, and the hypha collecting efficiency is improved; the problem that hyphae grow in the soil between the front outer membrane and the front inner membrane instead of being collected on the membranes when the interval between the front outer membrane and the front inner membrane is far is solved.

The invention relates to the technical field of paecilomyces cicadae, in particular to an efficient paecilomyces cicadae fermentation process based on a composite culture medium, which comprises the following steps: inoculating paecilomyces cicadae strain into the composite culture medium; then regulating and controlling the temperature and the ventilatory capacity according to the fermentation stage; meanwhile, adding a metabolism accelerant after fermenting for 72 hours; and after fermentation is completed, obtaining fermentation liquor, and separating to obtain paecilomyces cicadae. Nutrient substances are rapidly conveyed through the liquid phase to promote rapid proliferation of hyphae, meanwhile, the adsorption and expansion capacity of the hyphae is enhanced through the porous structure of the solid phase, and therefore the density of the hyphae and the synthesis efficiency of metabolic active substances are improved; a precise temperature control strategy is matched with physiological transformation from hypha growth to product accumulation, enzyme activity is guaranteed, and heat damage is reduced; and the metabolism accelerant is added to synergistically stimulate the high-efficiency expression of the target metabolite, so that the yield and quality of the target product are improved.

The invention relates to the technical field of strain culture, in particular to a culture formula of a golden stropharia rugosoannulata strain and a preparation method of the golden stropharia rugosoannulata strain. The culture medium comprises a culture material for strain propagation and a culture material for fruiting production, wherein the culture material comprises 30%-40% of sawdust, 15%-20% of bran, 1%-2% of cane sugar, 0.5%-1% of gypsum powder, 0.2%-0.5% of calcium carbonate, 0.3%-0.5% of yeast extract and the balance of water; the cultivation material comprises 60%-70% of corncob, 15%-25% of bran, 1%-2% of gypsum powder, 0.5%-1% of calcium carbonate and the balance of water; according to the method, hyphae grow fast, infectious microbe cannot be generated, the cultivation period is shorter, and infectious microbe cannot be generated during cultivation.

The invention discloses a planting frame self-adaptive adjusting mechanism for ganoderma lucidum cultivation and a control method, and relates to the technical field of environment regulation, a planting frame is divided into a plurality of three-dimensional cultivation spaces, each three-dimensional cultivation space comprises a cultivation layer distinguished based on a ganoderma lucidum growth cycle, a middle layer is a hypha growth period cultivation layer, and a middle layer is a hypha growth period cultivation layer; the upper layer is a sporocarp differentiation period cultivation layer, and the lower layer is a sporocarp mature period cultivation layer, so that environmental parameter adjustment and region adjustment can be carried out in time, and it is guaranteed that the whole planting process can still be stably and efficiently operated in the case of batch change.

The invention discloses a method for inducing rapid formation of Chinese saprophytic boletus primordium, and belongs to the technical field of edible fungus cultivation. The method comprises the following steps: activating a boletus sinensis strain JSJ-Bx1, inoculating the boletus sinensis strain JSJ-Bx1 into a culture medium, and carrying out dark culture; the preservation number of the boletus sinensis strain JSJ-Bx1 is CGMCC (China General Microbiological Culture Collection Center) No.42007, and the boletus sinensis strain JSJ-Bx1 is preserved in the China General Microbiological Culture Collection Center; performing liquid fermentation culture on the hyphae which are activated and are almost overgrown on the flat plate to obtain fermentation liquid; inoculating a cultivation material with the fermentation liquid, performing dark culture until the bottle / bag is full of hyphae, covering the bottle / bag full of the hyphae with soil, covering the surface with non-woven fabric as a protective layer, and performing hypha culture and light culture for 3-5 days to obtain a soil-covered fruiting bottle / bag; performing light culture on the soil-covered fruiting bottle / bag in a fruiting room, and spraying a monopotassium phosphate solution during culture to promote mycelial growth and induce primordium formation. The method can effectively promote rapid growth of Chinese saprophytic boletus hyphae in an overburden layer, primordia are rapidly formed on the surface of the overburden layer, and harvesting can be performed 13-15 days after overburden.