11 results about "Hyphal growth" patented technology

The invention belongs to the technical field of biology, and particularly relates to application of serine in relieving heat stress of pleurotus ostreatus and a method. Under the heat stress condition, exogenous serine supply promotes pleurotus ostreatus hypha to recover growth, and the activity of antioxidant enzyme, the yield of sporocarp and the yield of nutrient substances are improved; when the activity of the PHGDH enzyme is inhibited, the serine synthesis is reduced; the hypha growth can be promoted by overexpressing the phgdh, and the enzymatic activity of the PHGDH can be improved; when acetyltransferases HAT, NAT1, NAT2 and NAT3 act on a K394 site of the PHGDH, the enzyme activity of the PHGDH can be improved, endogenous serine metabolism is activated to respond to heat stress, it is indicated that PHGDH-mediated serine metabolism plays an important role in corresponding heat stress of the pleurotus ostreatus, and the invention discloses a mechanism for improving heat resistance of the pleurotus ostreatus through the PHGDH-mediated serine metabolism. And a foundation can be laid for breeding high-temperature-resistant pleurotus ostreatus dominant strains.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The invention belongs to the technical field of microorganisms, and particularly relates to application of an oyster mushroom small heat shock protein (PoHSP20-6) gene in regulation and control of hypha heat resistance and a method for constructing an overexpression PoHSP20-6 gene oyster mushroom strain, and the nucleotide sequence of the PoHSP20-6 gene is shown as SEQ ID NO: 1. According to the invention, through a PoHSP20-6 overexpression vector, a PoHSP20-6 gene overexpression oyster mushroom strain is constructed and obtained. And it is proved that the hypha growth recovery capability of the overexpressed oyster mushroom strain under 40 DEG C high temperature stress is significantly improved, and the high temperature tolerance is significantly enhanced. The PoHSP20-6 gene and the oyster mushroom strain overexpressing the PoHSP20-6 gene provide key gene resources and technical support for fungus stress resistance breeding, can effectively solve the problem of poor hypha growth caused by high temperature stress in edible fungus cultivation, and have important application value in the edible fungus industry.

The invention belongs to the technical field of microorganisms, and discloses a pleurotus purpureus strain 162 and a cultivation method thereof. The preservation number of the strain 162 is CGMCC (China General Microbiological Culture Collection Center) No. 42041. The cultivation method comprises the following steps: 1) inoculating a pleurotus purpureus strain 162 on a mother strain culture medium to obtain a strain 162 mother strain; 2) inoculating the strain 162 mother strain to a stock culture medium to obtain a strain 162 stock; 3) inoculating the strain 162 stock seed to a cultivar culture medium to obtain a strain 162 cultivar; and 4) cultivating the cultivar of the strain 162 to obtain the sporocarp of the strain 162. The strain 162 is fast in hypha growth, short in after-ripening period, fast in fruiting, white in pileus, strong in adaptive capacity and short in harvesting period; the biological efficiency is high; the feed is high in nutritional value and has strong disease resistance to bacterial brown spot.

The invention provides a ganoderma strain SM07 and application thereof. The preservation number of the ganoderma strain (Ganoderma sp.) SM07 is CCTCC (China Center for Type Culture Collection) NO: M 20251816. The bacterial strain is developed in aerial hypha, rapid in hypha growth and high in hypha kinking property, straw can be used as a raw material to produce the biomass lining, and the biomass lining package is pure white and smooth in appearance, light and attractive in texture, has the loose and porous structural characteristic, is excellent in air permeability, can provide good buffering protection and can be used for preparing the biomass lining. And the quality stability in the tobacco leaf storage process is guaranteed. Besides, the environment-friendly characteristic is outstanding, the smoke biomass lining can be degraded by microorganisms in soil and converted into soil organic matter after being used, resource cyclic utilization of agricultural by-products is achieved, traditional plastic foam can be replaced, the problem of white pollution is avoided, and wide market prospects are shown in the field of green packaging.

This invention discloses a non-destructive sampling method for studying the interaction between AM fungi and mycelial bacteria. Step 1: Sterilized culture medium is filled into the root chamber and the mycelial growth chamber, and the outer end of the mycelial growth chamber is sealed; the inner end of the mycelial growth chamber is connected to the root chamber. Step 2: Corn seeds are planted in the culture medium in the root chamber, and AM fungal spores are added to the root chamber while simultaneously cultivating them using nutrient solution and water. Step 3: Collection is performed, consisting of collecting pure mycelia and collecting fresh soil affected by pure AM fungal mycelia. This invention uses non-destructive sampling under pot conditions to collect pure, uncontaminated AM fungal mycelia and mycelial soil affected by AM fungi for scientific research on mycelial interactions.

This invention relates to the field of pharmaceutical technology, specifically to the application of dehydronuciolin and fluconazole in the preparation of antifungal drugs. The in vitro synergistic effect of the combination of dehydronuciolin and fluconazole was determined using a checkerboard assay, showing a fractional inhibitory concentration (FIC) ≤0.5, confirming a strong synergistic effect. The in vivo efficacy of this drug combination was verified using a large wax moth infection model. Phenotypic experiments explored the synergistic mechanism, showing that the combination of dehydronuciolin and fluconazole significantly inhibited the hyphal growth, biofilm formation, and adhesion to host cells of drug-resistant Candida albicans. Transcriptome sequencing analysis confirmed these findings, showing that treatment with the combination of dehydronuciolin and fluconazole downregulated hyphae-specific genes, GPI-anchored cell wall proteins, and the ergosterol biosynthesis pathway, collectively weakening the virulence, surface integrity, and host recognition ability of Candida albicans, thereby reversing its drug resistance.

The present application belongs to the field of pharmaceutical chemistry, and particularly relates to Hypogin derivative and application thereof. The present application firstly simplifies the structure of Hypogin endogenous peptide, finds an active fragment, and designs and synthesizes Hypogin derivatives of series I. The Hypogin derivatives with better activity in series I are combined with cinnamic acid to design and synthesize compounds of series II. Research finds that the compound II-1 can better inhibit the hypha growth of Sclerotium rolfsii, and it is found that I-12 affects the signal pathway of PI3K of Sclerotium rolfsii, and the potential target is GPCR, RTK and PI3K of Sclerotium rolfsii. The cytotoxicity experiment shows that II-1 has low hepatocyte toxicity of human LO2, and conforms to the development direction of green bactericides. The Hypogin derivative has bacteriostatic activity, can be used as a basis for development of small peptide bacteriostatic agent, and has good application prospect.

The present disclosure relates to methods and compositions for genetically engineering microorganisms for improved control of pathogens such as bacteria and / or fungi, which can be applied in various fields such as medicine and agriculture. The genetically engineered microorganisms may comprise deletions or knockout at one or more genomic loci that confer the ability to reduce mycelial growth of the fungi, reduce sporogenesis of the fungi, and / or other benefits.

The invention discloses a hymenopellis raphanipes mutant strain M1 obtained through artificial mutagenesis, the hypha growth rate of the hymenopellis raphanipes mutant strain M1 is larger than or equal to 5.20 mm / d, the biological efficiency reaches 53.6%, the hymenopellis raphanipes mutant strain M1 has strong resistance to trichoderma harzianum, the proportion of first-grade mushrooms is larger than or equal to 40.6%, and the hymenopellis raphanipes mutant strain M1 is good in hereditary stability. The invention also provides a breeding method of the strain, a special liquid fermentation culture medium and an industrial cultivation process in an intelligent mushroom square cabin. The method solves the problems that traditional cultivation is long in period, high in pollution rate, difficult to factorize and the like, and a complete solution is provided for annual efficient production of oudemansiella oosporium.

The invention relates to the technical field of strain culture, in particular to a culture formula of a golden stropharia rugosoannulata strain and a preparation method of the golden stropharia rugosoannulata strain. The culture medium comprises a culture material for strain propagation and a culture material for fruiting production, wherein the culture material comprises 30%-40% of sawdust, 15%-20% of bran, 1%-2% of cane sugar, 0.5%-1% of gypsum powder, 0.2%-0.5% of calcium carbonate, 0.3%-0.5% of yeast extract and the balance of water; the cultivation material comprises 60%-70% of corncob, 15%-25% of bran, 1%-2% of gypsum powder, 0.5%-1% of calcium carbonate and the balance of water; according to the method, hyphae grow fast, infectious microbe cannot be generated, the cultivation period is shorter, and infectious microbe cannot be generated during cultivation.