496 results about "Hyphal growth" patented technology

The invention discloses a culturing method for sporocarp of Morchella. The culturing method comprises the following steps: fungal strain preparation, fungal strain culturing, soil treatment, sowing, covering and temperature preservation, management in the growth period of mycelia, fruiting acceleration treatment, management in a fruiting period, and harvesting. According to the invention, operation steps like drawing of fungal strains from a fungal strain bottle or a fungal strain bag, fungal strain hydration, and placement of a large amount of nutrition material bags on the surface of soil in a traditional technology are removed, so the field-cultivation or indoor-cultivation technique for the Morchella is more simple and convenient; the cost of the nutrition material bags is reduced by three fourth or more; the generation cost of cultivation of the Morchella is greatly reduced; the technical difficulty of the cultivation of the Morchella is greatly decreased; and economic benefits are greatly improved.

A lighting apparatus for controlling a plant disease is provided to reliably inhibit a spore formation and a hyphal growth of a filamentous fungi, reliably induce a disease resistance, prevent a leaf scorch, and promote a growth of a plant. A UV light source repeats a light irradiation which has an irradiation pattern of combining a light irradiation, in which UV-C and UV-B and a visible light are superimposed, with a visible light irradiation alone at least twice a day. The emission of UV-B and UV-C enables the reliable inhibition of the spore formation of the filamentous fungi, for example. Its intermittent emission prevents a plant from getting too used to ultraviolet rays, promotes a generation of an antibacterial substance, for example, and prevents a leaf scorch. The continuous emission of the visible light enables a promotion of a growth of the plant.

The invention provides a fermentation method for high cordyceps militaris biomass and high cordycepin content, which belongs to the technical field of bio-engineering. A cordyceps militaris strain is activated, inoculated to a first stage seed tank and further expanded to a fermentation tank, when a certain biomass is achieved in the fermentation tank, a biotic elicitor of Xylaria type endophytic fungus from binkgo biloba extract liquid and non-biotic elicitors of sodium acetate and ammonium sulfate are added for promoting the production of cordycepin in bacteria. The fermentation method adopts an optimized culture medium for effectively increasing the mycelial growth of cordyceps militaris; furthermore, under the regulation role of the elicitors, the cordyceps militaris biomass and the cordycepin content in the bacteria are respectively increased by 1.76 times and 7.14 times compared with the contrast culture medium without the addition of the elicitors and respectively increased by 2.35 times and 23.25 times compared with the ordinary improved PDA. The fermentation method greatly improves the production amount of cordyceps militaris mycelia and the content of the active characteristic ingredient, thereby having broad market prospect.

The invention relates to bacillus amyloliquefaciens, the preservation number of which is CCTCC M 2015299, and which is named as bacillus amyloliquefaciens XK-2. The invention further provides a screening method of the bacillus amyloliquefaciens XK-2 and an application of the bacillus amyloliquefaciens XK-2 in inhibiting watermelon fusarium oxysporum, rhizoctonia solani and walnut root rot pathogenic bacteria as well as a bacillus amyloliquefaciens XK-2 bactericide. The bacillus amyloliquefaciens XK-2 is a watermelon endophytic bacterial which is safe to the human beings, animals and crops, environment-friendly, relatively wide in antibacterial spectrum, has a good inhibition effect on common soil-borne pathogen,watermelon fusarium oxysporum, rhizoctonia solani and walnut root rot pathogenic bacteria, has a significant control effect on watermelon fusarium wilt, has an obvious inhibition effect on the growth of watermelon fusarium oxysporum hypha, slows down the growth of hypha, facilitates the distortion syndrome of the hypha and is wide in application prospect in biological control of plant diseases.

The invention relates to a method for producing the edible fungus mycorrhizal seedling of a mycorhiza. The method is characterized by comprising the following steps: the mycelium of a target mycorrhizal fungus and the mycorhiza of a non-competitive mixed fungus are synthesized into a culture medium, are uniformly mixed, are spread in a container and are cultured under a condition which is suitable for the growth of hypha, wherein the surface of the container is disinfected by a potassium permanganate solution or sterilized under high temperature and high pressure, and the lower part of the container is spread with a layer of non-competitive mixed fungi or media sterilized under high temperature and high pressure; and a sterile germinated host plant seed of which the surface is disinfected and the culture medium culturing the target hypha are mixed and cultured under a proper ecological condition until the mycorhiza of a tree seedling root is formed. The synthesis experiment of the edible fungus mycorrhizal seedlings of various mycorhiza shows that the method has simple and effective operation and high efficiency, is very suitable for popularization and application in mass production, can be used for producing mycorrhizal seedlings at any time in one year and has obvious economic benefits.

The invention provides a preparation method for an edible fungus culture medium. The preparation method comprises the following steps: 1) adding water mulberry branch powder to perform enzymolysis; 2) adding a compound enzyme preparation and uniformly mixing, fermenting and performing enzymolysis at the room temperature; and 3) adding 28-42 parts by weight of cotton seed hulls, 14-20 parts by weight of cassava vinasse, 12-18 parts by weight of bran, 2-5 parts by weight of lime, 1-5 parts by weight of gypsum and 1-5 parts by weight of calcium superphosphate into 42-78 parts by weight of mulberry branch powder obtained in the step 2) in sequence. An edible fungus cultural method is characterized by comprising the following steps: 1) adding a nutrient solution; 2) sterilizing; and 3) inoculating and culturing funguses. The edible fungus culture medium is low in contamination rate, high in fungus growing speed, good in mushroom-stick quality, thick in hypha growth, strong in growth power, good in receiving luminosity and good in disease resistance.

The invention belongs to the field of utilization of organic matters, and in particular relates to an edible straw rotting fungus culture medium and a preparation method thereof. The edible straw rotting fungus culture medium comprises the following components in percentage by weight: 40 to 50 percent of primary fungus chaff, 30 to 50 percent of xylitol residue, 5 to 20 percent of bran, 0.2 to 2 percent of calcium superphosphate, 0.2 to 1 percent of corn flour and 0.5 to 1 percent of gypsum. Lime milk and water are added into the medium, the pH is adjusted to be 8 to 10, and the water content is controlled to be 55 to 70 percent. The edible fungus culture medium is high in hypha running speed, hypha exuberantly grows, and the sundry fungus contamination rate can be greatly lowered; produced edible fungi have various nutritional characteristics of edible fungi produced by an ordinary culture material, and have a high edibleness and medicinal value.

The invention discloses a distiller grain composite culture medium and an application thereof in factory-like white needle mushroom cultivation. The culture medium comprises the components in part by mass of 5-20 parts of distiller grains, 30-40 parts of rice bran, 5-10 parts of bran, 30-40 parts of corncob, 3-5 parts of corn flour, 5-10 parts of sugar beet pulp, 1-3 parts of shell powder and 1-3 parts of light calcium carbonate, the moisture content is 66% to 68%, and the pH is 6.2 to 6.8. The distiller grain composite culture medium has the advantages that the granularity of the culture medium is uniform, the breathability is good, the growth speed of hypha is rapid, and the culture period is short. Compared with a traditional needle mushroom culture medium and a culture manner, the production period can be shortened by 2-8 days, the average per unit yield is improved by 50-100 g, the uniformity and the whiteness are good, and the product appearance is improved greatly.

The invention discloses a culture method of black fungus rich in seabuckthorn flavonoids and a culture medium thereof and relates to the culture method of the black fungus and the culture medium thereof. The culture method can solve the problems that the hyphae growth speed during the culture process by using the existing culture method is slow, and the content of total flavonoids of the obtainedblack fungus is low. The method is as follows: 1, preparing the culture medium; 2, carrying out high-pressure or normal-pressure damp heat sterilization after bagging and sealing the culture medium, and then carrying out natural cooling; and 3, inoculating a black 29 secondary strain into a bacterial bag after cooling, then disinfecting the bacterial bag, cutting an opening, carrying out centralized germination and growing management, harvesting till a fruiting body of the black fungus grows to 80% of mature and drying in the air, thereby obtaining the black fungus rich in seabuckthorn flavonoids. The obtained black fungus rich in seabuckthorn flavonoids can effectively treat and prevent hypertension and hyperglycemia, suppress platelet aggregation and heart failure and increase the healthcare effect of the black fungus. The hyphae growth speed during the culture process is fast. The obtained product has high yield.

The invention discloses a mushroom cultivating material which is characterized in that bamboo sawdust, bamboo shells and biogas residues are used as raw materials, and the water content of the cultivating material is 62-65%. The invention also provides a manufacturing method of the cultivating material. Compared with the prior art, the mushroom cultivating material has the advantages that firstly, the cultivating material is reasonable in proportion, and a fungous material keeps certain air permeability, so that the growth speed of mycelia is increased, and the sackful time is shortened; secondly, bamboo sawdust particles are hard in texture and resistant to decomposition, and the biogas residues contains comprehensive nutrients including nitrogen, phosphorus and potassium, so that the growth of mushroom is promoted, the yield of a product is increased, and the quality of the product is improved; and thirdly, wood is replaced with bamboos and bran is replaced with the biogas residues, so that the source of a cultivating raw material is widened, and the production cost is reduced. The mushroom cultivating material has the social benefits that wood dust is replaced with the bamboo sawdust and the bamboo shells, so that part of contradiction between a fungus and forestry can be alleviated, waste materials can be changed into things of value, and energy conservation and emission reduction can be favorably realized.

The invention provides a culture medium for selenium-rich organic edible fungi, which is mainly prepared from the following raw materials in parts by mass: 60-90 parts of culture main material, 10-25 parts of carbon organic fertilizer, 1-5 parts of selenium-rich nutrient element, 1-6 parts of root irrigation culture agent, 0.2-0.5 part of compound probiotic fertilizer and 0.1-0.5 part of plant growth promoter. The method for culturing selenium-rich organic edible fungi by using the culture medium comprises the following steps: mixing the plant growth promoter and compound probiotic fertilizer, and dissolving in water to prepare a liquid; mixing the rest raw materials, adding the liquid, and fermenting to obtain the culture medium; and uniformly mixing a strain with the culture medium for inoculation, culturing for 10-15 days until the hypha are overgrown, and reinoculating the hypha. The culture medium has the advantages of reasonable component proportioning and high growth speed of hypha.

The invention provides a method for cultivating a liquid-state morchella strain. The method includes the steps that a liquid-state morchella strain culture medium is provided; a stock morchella strain is inoculated into the liquid-state morchella strain culture medium for conducting airtight culture; after hyphae of the stock morchella strain germinate, the liquid-state morchella strain culture medium is shaken once every day till a morchella cultivated species is obtained. According to the method for cultivating the liquid-state morchella strain, operation is easy, the cultivated hyphae of the morchella cultivated species grow fast, the produced hyphae are even and neat, contamination by other strains is small, and the artificial cultivation survival rate and yield of morchella are increased; moreover, the culture process is simple and easy to implement, and cost is low.

The invention provides a pleurotuseiorinopieutas cultivation material which is characterized in that tea seed shells and tea fruit shells are used as main raw materials and are supplemented with corn cores, bran, tea seed meal, a gypsum powder and other auxiliary materials, and the water content of the cultivation material is 63%-65%. The invention also provides a production method of the cultivation material. Compared with a wood dust cultivation material, the pleurotuseiorinopieutas cultivation material has the advantages that: one, the cultivation material has good gas permeability and strong water holding capacity, the growth speed of pleurotuseiorinopieutas mycelia is fast, and the sackful time is shortened; two, through natural accumulation of fermentation, lignin, cellulose, hemicellulose, a pectic substance and other substances in the tea seed shells and the tea fruit shells are decomposed into simple substances which are beneficial for the pleurotuseiorinopieutas mycelia to absorb, the formation of pleurotuseiorinopieutas fruiting body primordia is promoted, so that the effective mushroom number is increased, and the yield is increased by 30% to 40%; and three, channels for cultivation raw materials are broadened, and the production cost is greatly reduced.

The invention provides Paenibacillus polymyxa (named as Paenibacillus polymyxa WK-3) with the collection number of CCTCC M2015298 and further provides a screening method of the Paenibacillus polymyxa WK-3, application of the Paenibacillus polymyxa WK-3 in inhibition of rice sheath blight, watermelon fusarium wilt and hyphal cluster root-rot sickness and a Paenibacillus polymyxa WK-3 bactericide. The Paenibacillus polymyxa WK-3 is an endogenous strain of rice, is safe to human bodies, livestock and crops, environment-friendly and relatively wide in antibacterial spectrum, has a relatively good antibacterial effect on the growth of mycelia of common soil-borne pathogens such as the rice sheath blight, the watermelon fusarium wilt and the hyphal cluster root-rot sickness, has the antibacterial rates of respectively more than 85.4%, more than 81.8% and more than 73.9% for the rice sheath blight, the watermelon fusarium wilt and the hyphal cluster root-rot sickness and has a remarkable effect of inhibiting the growth of mycelia of the rice sheath blight and generating mycelia malformation; the further prepared bactericide has a remarkable effect of preventing and controlling rice sheath blight; and the Paenibacillus polymyxa WK-3 is short in production period and has a very wide application prospect in biological prevention and control of plant diseases.

The invention discloses a domestic fungus medium, a preparation method thereof and a domestic fungus culture method. The domestic fungus medium comprises, in parts by weight, 75-86 parts of wood chips, 12-20 parts of wheat bran, 1-5 parts of a yeast fermentation product and 0.5-2 parts of gypsum, wherein in the yeast fermentation product, the weight content of organic matter is 50% or more, the weight content of nitrogen phosphorus potassium nutrients is 10% or more, and the weight content of amino acid is 6% or more. By adding the yeast fermentation product into the medium for replacing sugar and part of wheat bran in a conventional domestic fungus medium, the production cost of domestic fungus is substantially reduced when high-quality domestic fungus is guaranteed to be cultured. The domestic fungus medium is low in polluting rate, the yeast fermentation product is high in contents of organic matter, amino acid and nitrogen phosphorus potassium nutrients, beneficial microbes and granular organic fertilizer in assistant materials can satisfy strain growth of domestic fungus, bacteria fermentation speed is fast, bacteria stick quality is good, and mycelia grow densely, are strong in growing power, high in received luminosity and good in disease prevention performance.

The invention discloses a method for preparing a degradable buffer material by using macro fungi mycelia. The method comprises the following steps: smashing agricultural and forestry wastes such as cottonseed hulls, bran and sawdust which serve as raw materials, adding a fixed amount of matrix nutrient into the raw materials to prepare a mycelium growing matrix, and putting the matrix into a sealable mold; after high-pressure wet heat sterilization is completed, inoculating a liquid strain of macro fungi into the mold; wrapping and bonding matrix micro particles to form a whole according to the feature that the mycelia are mutually twisted for growth in the matrix; after the mycelia grow to a certain extent, performing mold release growth to enhance the water resistence and the durability of the mycelia, and finally drying the mycelia to obtain the material. The material prepared by the method disclosed by the invention is extremely high in buffering property and mechanical property, can be completely and naturally degraded, and has a practical significance for comprehensive use of agricultural and forestal production wastes and protection of the environment.

The invention discloses a preparation method of an edible mushroom wood plant mother culture medium and a product thereof. The preparation method comprises the steps of: 1, with deciduous species wood as raw materials, processing the deciduous species wood into a wood plant; 2, soaking the wood plate in a nutrient solution with nutrients required by the growth of the hypha, fishing out and then dripping water on the surface until no water drops exists; and 3, loading the wood plant prepared in the step 2 into a high-temperature resistant glass bottle, filling in gaps with compost, covering and flattening with the compost, plugging cotton balls, and sterilizing to obtain the edible mushroom wood plant mother culture. The edible mushroom wood plant mother culture has a long storage period and ageing resistance, and can be stored for 3-5 months at a normal temperature of 5-25 DEG C, thereby improving the culture preservation effect by 10 times. The edible mushroom mother seed cultured bythe culture medium has the advantages of vigorous hypha, high survival rate, strong vitality and rapid hypha development.

The invention relates to a new strain of edible fungus, belonging to the technical field of edible fungus development and utilization. The new grey variant strain of edible fungus with red pleurotus: Pleurotus djamor (Rumph.: Fr.)Boedijn, is the new strain of edible fungus selectively bred through single spore cross breeding and has the number No.1933 in CGMCC (China General Microbiological Culture Collection Center). The strain is characterized in that the color of sporophore is stable grey white, the whole sporophore is pure and transparent, the cellulose content is 5-12%, and the strain has favorable mouth feel and is used for producing the edible fungus in the high temperature season. Mycelium grows very fast and has strong ability in fighting bacteria and also has developed aerial hyphae. The hypha has clamp connection and the growth temperature of 3-45 DEG C; the temperature of sporophore differentiation is 18-35 DEG C, and the new grey variant strain is typical high temperature edible fungus. Straws, saw dust, bran, and the like are selected as cultivation materials. The edible fungus is cultivated in March to November. Cultivation materials produced in scale do not need high pressure or normal pressure sterilization, and the sporophore output is 15-25%. The strain is newly selected and bred edible fungus and has application value in the edible fungus field and ornamental fungus field.

A method for using liquid strains to cultivate agrocybe cylindracea particularly includes: preparing agrocybe cylindracea liquid strains according to conventional methods with compost made by hard and mixed saw dust, cottonseed hull, bran, corn powder, calcium carbonate and gypsum, preparing cultivating media, and sequentially performing bagging, sterilization, liquid strain inoculation, cultivation, fruiting management and harvesting. By the method scientific and reasonable in raw material proportion, high yield and quality of agrocybe cylindracea are guaranteed. Preparation of the liquid strains only needs 3-5 days, which is 1/10 of the cycle of the methods using solid strains. The agrocybe cylindracea can fill the whole agrocybe cylindracea cultivation bags for 18-20 days under the cultivation at 25 DEG C, which is 50% of the growing period of the methods using solid strains. The method has the advantages that cultivation cycle is shortened greatly by using the liquid strains, due to the fact that the liquid strains are fast in sprouting and compost absorption, short in mycelial growth time, multiple links of intermediate seed production are omitted, multi-season cultivation in one year can be achieved, and economical benefits are multiplied.

The invention provides a Hericium erinaceus cultivation material, which is characterized by comprising main raw materials of chestnut branch sawdust, bud shell shreds and chestnut shell shreds, and accessory materials of cob, wheat bran, soybean meal and gesso; the pH value of the cultivation material is adjusted by potassium dihydrogen phosphate to 5.5-5.7; and the water content of the cultivation material is 63-65%. The invention also comprises a manufacturing method of the cultivation material. Compared with an existing sawdust and cottonseed hull cultivation material, the cultivation material provided by the invention has the following advantages: 1, the cultivation material has suitable pH value, realizes vigorous and compact growth of mycelia and shortens time for filling a bottle; 2, the chestnut branch sawdust, bud shell shreds and chestnut shell shreds are subjected to daylight insolation, accumulation aging and biological fermentation to remove poisonous bacteriostatics and degrade macromolecular substances, so as to benefit absorption of the Hericium erinaceus mycelia; 3, the Hericium erinaceus has good fruiting performance, good product properties and high biological efficiency; and 4, the method widens the channels of cultivation raw materials and substantially reduces the production cost.

The invention discloses a separating culturing method of Chinese wild morin mushroom strain in the bacterial separating culture technical domain of fungi, which comprises the following steps: (1) making the culture medium of test tube oblique plane according to the proportion and dispense with 220-260g potato, 18-25g glucose, 0. 8-1. 2g potassium biphosphate, 0. 4-0. 6g magnesium sulfate, 18-12mg vitamin B, 80-100g mulberry branch, 18-20g agar, 1000mL water with pH value at 6-7. 0; (2) separating to culture: disposing the solid of morin; separating morin; culturing mother seed. The invention can accelerate the growth and breeding of hypha effectively to improve the activity of the strain, which realizes the high production of hypha fermented by morin liquor to reach 8. 83g dried hypha in the 1000ml liquor evenly.

The invention relates to a pholiota nameko cultivation material which is characterized in that broad bean hulls are used as a main raw material, cotton linters, bran, corn flour, gypsum powder and the like are used as auxiliary materials, and the water content of the cultivation material is 65-66 percent. The invention also relates to a method for preparing the cultivation material. Compared with a saw dust cultivation material, the pholiota nameko cultivation material has the advantages that 1, the cultivation material is reasonable in matching of coarse sand fine grains, and the air permeability and water binding capacity of the cultivation material are increased, so that the growth rate of pholiota namekohypha is increased, and the bagful time is shortened; 2, the cultivation material has a reasonable carbon nitrogen ratio and balanced nutrition and promotes the growing development of sporocarp, and the yield is increased by 20-30 percent; 3, the cultivation raw-material channel is broadened, and the production cost is greatly reduced. The pholiota nameko cultivation material compatibility and the method have the social benefits that a broad bean hull substitution is used for cultivating pholiota nameko, one part of fungus-forest contradiction can be eased, waste is turned into wealth, and energy conservation and emission reduction are facilitated.