The present invention provides a high-yield
molecular marker and breeding method combining the traits of
common Camellia oleifera seedlings, belonging to the technical field of
gene breeding. The present invention has developed two high-yield molecular markers, ISSR815 and ISSR820, which are closely linked to the traits of one-year-old seedlings cultivated from
hybrid seeds of
common Camellia oleifera, and obtained four
gene loci, 815-6, 815-3, 820-7, and 820-8. Combining the three indexes of
seedling height, height-
diameter ratio, and
water use efficiency of one-year-old seedlings cultivated from
hybrid seeds, high-yield excellent families and plants of
common Camellia oleifera are selected from 19
hybrid families in the natural
population, with a selection efficiency of 75% and a correct rate of 100%. According to this method, high-yield families of common
Camellia oleifera are obtained, with high yields. The average yield per unit crown area is 2.91 kg / m 2 , which is much higher than the national
industry standard of 1.2 kg / m for the average yield per unit crown area 2 . In each hybrid generation, high-yield varieties can be successfully screened out within two years, greatly shortening the breeding cycle compared with the more than ten or twenty years of the existing technology, and solving the problems of long breeding cycle, poor stability, and low efficiency in the existing
Camellia oleifera cross-breeding