250 results about "Indel" patented technology

The invention relates to an approach for introducing one or more desired insertions and/or deletions of known sizes into one or more predefined locations in a nucleic acid (eg, in a cell or organism genome). They developed techniques to do this either in a sequential fashion or by inserting a discrete DNA fragment of defined size into the genome precisely in a predefined location or carrying out a discrete deletion of a defined size at a precise location. The technique is based on the observation that DNA single-stranded breaks are preferentially repaired through the HDR pathway, and this reduces the chances of indels (eg, produced by NHEJ) in the present invention and thus is more efficient than prior art techniques. The invention also provides sequential insertion and/or deletions using single- or double-stranded DNA cutting.

The invention discloses a method for predicating a homologous recombination deficiency (HRD) mechanism and a method for predicating response of patients to cancer therapy and relates to the field of biological information predication. The method comprises the step of judging whether a tumor sample has homologous recombination deficiency or not according to one or more comprehensive values in a large-segment INDEL (Insertion/Deletion) fraction, a copy number variation fraction and a tumor mutation load fraction, wherein the comprehensive values can also comprise a loss of heterozygosity variation fraction. By adopting the method disclosed by the invention, predication of a chromosome large-segment structure, a chromosome gene type number, a chromosome gene copy number, a chromosome variation interval and abnormal loss of heterozygosity and chromosome telomeric imbalance is realized, so that an evaluation range is more complete and HRD can be accurately predicated; the comprehensive values also can be used for determining whether the patients have response to a therapeutic regimen containing one or more of a PARP (Poly Adenosine Diphosphate Ribose Polymerase) inhibitor, an DNA (Deoxyribonucleic Acid) injury inhibitor, a topoisomerase II/II+inhibitor, a topoisomerase I inhibitor and radiotherapy; the method is simple and has wide general applicability.

Compositions, methods and kits are disclosed for use in simultaneously amplifying at least 20 specific STR loci of genomic nucleic acid in a single multiplex reaction, as are methods and materials for use in the analysis of the products of such reactions. Included in the present invention are materials and methods for the simultaneous amplification of 23 and 24 specific loci in a single multiplex reaction, comprising the 13 CODIS loci, the Amelogenin locus, an InDel and at least six to ten additional STR loci, including methods, kits and materials for the analysis of these loci.

The invention discloses a compound amplification kit for InDel genetic polymorphic sites of human euchromosome and Y chromosome and application thereof. The kit comprises 47 pairs of euchromosome InDel site loca, 2 pairs of Y chromosome InDel site loca and a pair of specific amplification primers of a sex determination gene. The kit can be used for human individual recognition, paternity identification and degraded detection material recognition. The kit comprises 49 InDel sites which are relatively balanced in types and one sex determination gene. By adopting a six-colored STR fluorescence detection system, the kit is higher than the disclosed legal medical InDel detection kit. The kit disclosed by the invention is suitable for detecting Chinese groups. An amplification system of 50 sites is short in amplification fragment, and the amplicon is controlled at 200bp, so that the system is suitable for InDel typing of high degraded detection material; the amplification system improves the detection numbers of sites in the degraded detection material; the two Y-InDel sites introduced into the system plays an auxiliary judging role on the sex determination gene Amelogenin.

The invention belongs to the technical field of biological detection, and in particular relates to a fluorescently-labeled insertion/deletion (InDel) genetic polymorphism locus composite amplification system and application thereof. The application comprises the following steps of: selecting 35 InDel loci, designing polymerase chain reaction (PCR) amplification primers of the 35 InDel loci, matching fluorescein labels of the PCR primers, compositely amplifying and detecting the 35 InDel loci, and the like. The system can analyze 35 InDel genetic polymorphism loci by the composite amplification. The 35 InDel loci are labeled by FAM, HEX, TAMRA and ROX fluorescein with four different colors respectively. The composite amplification system based on the invention can be made into a kit, serves as an InDel locus fluorescence composite amplification kit with Chinese characteristics, and is applied to the fields, such as triplet paternity test, doublet paternity test, grandparent and grandchild test, sibling test, individual recognition, disease diagnosis, anthropology and the like.

The invention discloses a composition for modifying a nucleotide sequence, a method and application, and relates to the technical field of gene editing. The composition comprises a first vector and asecond vector, wherein the first vector is provided with the following expression elements, such as a cytosine deaminase expression element, an adenine deaminase expression element and a mutant Cas enzyme expression element; the second vector is provided with the following expression elements, such as a gRNA (guide ribonucleic acid) expression element and a uracil glycosidase inhibitor expressionelement. The composition is used for modifying the C bases at the 1st to 16th sites at the upstream of PAM (protospacer adjacent motif) of the target nucleotide sequence, so that the transformation ofC/G to T/A is realized. Compared with the existing gene editing technique, the composition and the method have the advantages that the working window is broader; the DSB (double-strand break) and indels (insertion/deletion mutations) are not introduced, the off-target effect is very low, the safety is higher, and the like.

The invention discloses a mapping result of an eggplant fruit color epistatic gene D. The gene D is mapped in a position near a nucleotide sequence Sme2.5_00538.1 on a No.10 chromosome of the eggplant. Based on the re-sequencing result of male and female parents, an InDel molecular marker closely linked with the eggplant fruit color epistatic gene D is obtained near the nucleotide sequence Sme2.5_00538.1 of the No.10 chromosome, and is named as InDel22522; the genetic distance of the marker from the epistatic gene D is 0.61 cM. The nucleotide sequence of the molecular marker InDel 22522 in the female parent has one 29-basic-group InDel more than that in the male parent. The mapping result on the eggplant fruit color epistatic gene D provided by the invention lays the foundation for the precise positioning and cloning of the eggplant fruit color epistatic gene; the InDel 22522 molecular marker and a molecular marker amplification primer can be applied to eggplant fruit color breeding practice in a way of simplicity, convenience, high speed and high flux; the eggplant fruit color property improving process is accelerated.

The invention relates to the field of molecular breeding, and in particular to an insertion/deletion (InDel) molecular marker AAD closely-linked to the tomato anthocyanin absent character and application thereof. The physical position of the InDel molecular marker closely-linked to the tomato anthocyanin absent character is No. 45339693 base to No. 45343097 base on chromosome 2 of tomato. The invention can replace a traditional method of selecting a green plant to ensure that a breeding material of tomato contains an anthocyanin absence (AA) mutation site. An AA site hybrid plant in the breeding material with normal phenotype is assist-selected by mean of the molecular marker, the breeding period can be shortened, and the breeding efficiency can be improved.

The invention belongs to the field of molecular biology and particularly relates to a molecular marker assisted breeding method of a new rice variety with resistance to rice blast and special primers thereof. The molecular mark is a co-dominant molecular mark Indel-1 of the rice gene Pi65(t) with resistance to rice blast and is a nucleotide sequence which is amplified from total DNA of rice by using a primer pair SEQ ID NO: 1 and SEQ ID NO: 2. The method can be applied to molecular marker assisted selection of Pi65(t) in resistance breeding of the resistance to blast of rice, so that the efficiency of the anti-disease variety breeding is improved and the workload of field identification is reduced.

The invention provides a fluorescent multiplex amplification system of 37 Y-STR gene locuses and a Y-Indel, a kit and application thereof, belonging to the technical field of biological detection. Thekit can amplify and analyze 37 Y-STR gene locuses and a Y-Indel at the same time. Six colors of fluorescence are used for marking, the size of the amplified product is 70-490bp, the operation is simple, and very high cumulative individual discernment capability and cumulative probability of paternity exclusion are realized.

The invention belongs to the technical field of tumor gene detection and provides a high-flux capture sequencing based somatic mutation detection method and device for tumor tissue single samples, andfurther provides a computer readable medium used for operating the method and detection equipment. According to the invention, a normal copy number base line is established by selecting detected normal control samples, and a model capable of accurately screening stable conditions of single sample microsatellite sites is established by selecting the detected normal control samples; and compared with the prior art, the problem that tumor tissue mutation detection is carried out under the condition of no control samples can be solved, besides, multiple types of mutation such as SNV, INDEL, CNV,Fusion and MSI can be detected better, and calculation of tumor mutation burden (TMB) can be performed. The somatic mutation detection of the tumor tissue single samples can be realized under the condition of no control samples.

The invention relates to a kit used for diagnosing/predicting sudden cardiac death (SCD). The kit comprises one or more of a specific primer pair (SEQ ID No. 1-SEQ ID No. 6) used for detecting the number rs3917 insertion/deletion polymorphic site on a COL1A2 gene, a specific primer pair (SEQ ID No. 7-SEQ ID No. 12) used for detecting the number rs72014506 insertion/deletion polymorphic site on anMIR155HG gene, a specific primer pair (SEQ ID No. 13-SEQ ID No. 18) used for detecting the number rs113044851 insertion/deletion polymorphic site on a CTH gene and a specific primer pair (SEQ ID No. 19-SEQ ID No. 24) used for detecting the number rs397729601 insertion/deletion polymorphic site on a DSG2 gene. The kit has the advantages that the SCD risks of a patient are evaluated through the insertion/deletion polymorphic evaluation, the associated analysis of the genotype of the four sites can favorably provide molecular-genetics support and help for the diagnosis and prediction of the SCD,and the kit has high application value in the risk evaluation and diagnosis of the SCD; in addition, the kit is good in detection specificity, high in sensitivity and good in accuracy.

The invention discloses a molecular marker Indel-T-47 for close linkage with cucumber parthenocarpic main-effect QTL (quantitative trait loci), and belongs to the technical field of biology. The invention firstly discloses a molecular marker Indel-T-47 for close linkage with QTL Parth2.1, and further discloses a primer Indexl-T-47F/Indel-T-47R of the marker. In a segregating population containing Parth2.1, the marker is significantly related to parthenocarpy; in an all-female selfing line material containing the Parth2.1, a 155bp product can be amplified from a strong parthenocarpy strain by the marker primer Index-T-47F/Index-T-47R; the marker primer Index-T-47F/Index-T-47R is a peak marker of the Parth2.1, and is closely linked with the Parth2.1. The molecular marker for close linkage with the Parth2.1 disclosed by the invention can be applied to assistant breeding of an all-female cucumber parthenocarpy molecular marker.

The invention belongs to the field of molecular genetic breeding science, and provides a molecular marking method of gene loci related to peanut lateral branch angle, growth habit and plant types andapplication thereof. By designing a CAPS molecular marker, and by using a method combining primer amplification, enzyme digestion and agarose gel electrophoresis, the judgment of allelic variation ofgene loci related to the lateral branch angle, the growth habit and the plant types is realized. By labeling PM15-ID1 and PM15-ID2 with InDel, and by using the method of primer amplification and polyacrylamide gel electrophoresis, the marker-assisted selection of offspring with allelic variation of gene loci related to lateral branch angle, growth habit and plant types. The molecular marking method provided by the invention can be used for detecting the offspring of a breeding group constructed by using peanut creeping germplasm and upright germplasm as parents, can be used for rapidly identifying the allelic variation of gene loci related to lateral branch angle, growth habit and plant types of the offspring, can improve the selection efficiency of the gene loci, and can provide referencefor peanut plant type improvement and high-yield breeding.

The invention provides an InDel marker of a cucumber anti-cucumber-mosaic-virus-disease gene cmv and its application, belonging to the field of biotechnological assistant breeding. The nucleotide sequence of a primer InDel-cmv3-F/ InDel-cmv3-R for the InDel marker closely linked with the cucumber anti-cucumber-mosaic-virus-disease gene cmv is as described in the specification; a characteristic band amplified by the InDel marker and closely linked with the anti-cucumber-mosaic-virus-disease gene cmv has a length of 223 bp; and a characteristic band amplified by the InDel marker and closely linked with a cucumber-mosaic-virus-disease susceptibility gene CMV has a length of 245bp. The marker provided by the invention has the advantages of high efficiency and little limitation, improves the efficiency of breeding of anti-cucumber-mosaic-virus-disease cucumber materials and shortens a breeding cycle.

The invention belongs to the fields of agricultural biotechnology engineering and genetic breeding of vegetables, and particularly relates to molecular markers associated with male sterile mutation sites ms-15, ms-26 and ms-47 of tomatoes, a specific primer and application. The molecular markers comprise the co-dominance molecular marker MS15C used for detecting the SNP in the site ms-15, and theco-dominance molecular marker MS26D used for detecting InDels in the sites ms-26 and ms-47. The molecular markers can be employed to replace the traditional method that by selecting male sterile plants in the flowering stage, it is ensured that a tomato breeding material contains the male sterile mutation sites ms-15 or ms-26 or ms-47. The molecular markers can assist in selecting homozygous plants and hybrid plants containing the mutation sites ms-15 or ms-26 or ms-47 in the seedling stage, so that the breeding period is shortened, and the breeding efficiency is improved.

The invention discloses a set of mung bean InDel molecular markers and a development method thereof. The development method comprises the steps: mung bean parents are selected to be hybridized so as to construct a mung bean genetic mapping population; parent genomes are re-sequenced; parental sequencing data are subjected to quality control and then compared to mung bean reference genomes to screen differential sequences, then InDel loci between the parental genomes are screened, finally primers are designed for the InDel loci, and the multiple pairs of primers are obtained; the polymorphic primers evenly covering 11 chromosomes of mung beans are selected, and a plurality of filial generation plants are selected at random to construct a mung bean genetic linkage map; and the hundred-grainweight per plant is measured, quantitative trait loci (QTL) for controlling and adjusting the hundred-grain weight are identified, and QTL intervals and the linked markers are obtained. The success rate and the polymorphic primer ratio of primer design are increased, polymorphism is better, and the genetic linkage map constructed through the InDel markers can be used for identification of the QTLof quantitative traits and is beneficial to important gene identification of the mung beans and assistant breeding of the molecular markers.

The invention discloses a compound amplification detection kit containing forty InDel genetic polymorphic sites of human X chromosome. The forty InDel sites of the X chromosome and an individual identification location point are amplified simultaneously by a primer group. Compared with a traditional STR genetic marker, an insertion deletion genetic marker used in the compound amplification detection kit has the advantages of low mutation rate and high sensitivity; and the polymorphism detection sites involved in the compound amplification detection kit combine the advantages of an InDel genetic marker and the special genetic characteristics of the X chromosome, and effective supplementary means can be provided for identification of difficult or special cases.

The invention provides a fluorescently-labeled X-InDel locus composite amplification system. By means of the system, eighteen insertion-deletion genetic polymorphism loci on an X chromosome can be compositely amplified, wherein the eighteen loci are respectively labeled with three fluoresceins FAM, HEX and TAMRA. The composite amplification system is high in sensitivity and individual identification rate, is high in polymorphism, is good in stability and repeatability, is accurate in typing results and can satisfy a practical requirement. A kit can be manufactured on the basis of the composite amplification system, can be used for paternity test, dyad paternity test, grandparent and grandchild test, sibling test and individual identification. New technologies can be provided for the fields such as anthropology, medical genetics and the like.

The invention proposes a lymphoma gene capture chip, which comprises: a substrate and a probe, wherein the probe is arranged on the substrate, and specifically recognizes at least one of genes represented by table 1. According to the present invention, the chip comprises the related Driver Genes, the high frequency mutation genes, the important genes in five cancer-related signaling pathways, thetargeted drug-sensitive and chemotherapeutic drug-sensitive genes and the drug-resistant related genes in various subtypes of common highly-incident lymphoma, can be used for the detection of the single-nucleotide site variation (SNV), the Indel insertion or deletion (Indel), the gene copy number variation (CNV) and the chromosome structural variation (SV) of lymphoma-associated genes, can achievethe detection of various subtypes of lymphoma, and can be effectively used for the early screening, the assisted diagnosis, the medication guidance and the recurrence monitoring of lymphoma.

The invention discloses a major QTL, qTLA-9, which is used for regulating the angle of tilt of paddy rice leaves, and locates between the mark Indel Tal-1 and on Indel Tal-2 on the ninth chromosome, and the genetic distance is 74.80 cm-98.33 cm, the physical distance is 17448080 bp-2293 8953 bp. 2293 8953 bp. The invention also provides a mark Indel of the major QTL, qTLA-9, which is used for regulating the angle of tilt of paddy rice leaves; the invention also provides the application of the major QTL: by developing the molecular marker closely linked to the major QTL, the test result that whether there are leaf inclination-related QTLs in rice varieties or strains can be attained, thereby speeding up the breeding process of excellent rice varieties.

The invention discloses a gene detection kit which is used for detecting cell chimerism or individual recognition, in particular relating to an oligonucleotide array sequence, a kit and a method usedfor detecting cell chimerism or individual recognition. The kit utilizes gene insertion/deletion polymorphism (INDELs) and site-specific fluorescent quantitative PCR to quantitatively detect that different individuals of human beings have chimerism or microchimerism of foreign body cells, which can be used for the detection of mother and son/daughter cell microchimerism, cell chimerism of donor/receptor after the transplantation of hematopoietic stem cells or other organs and residual diseases after the transplantation of hematopoietic stem cells of leukemia, and for the forensic individual identification, paternity test and the like. The kit in the invention has good specificity and high detection sensitivity, can accurately and quantitatively detect with convenient operation and with norequirement of special devices; and when adopting the method in the invention to carry out individual identification detection, only agaroseelectrophoresis is needed to distinguish the results.

The invention belongs to the field of medicolegal genetics, particularly relates to a forensic medicine composite detection kit based on 20 multiple insertion/delection genetic markers, and aims to achieve the purpose of using multiple insertion/delection genetic markers to perform forensic medicine affiliation identification and individual identification on anthropobiology samples. The adopted technical scheme is that the forensic medicine composite detection kit comprises a complex amplification primer mixture and an allelic ladder mixture which are packed separately; the complex amplification primer mixture totally comprises 41 pieces of amplification primers marked by the 20 multiple insertion/delection genetic markers; the allelic ladder mixture is composed of allelic ladders which are marked by the 20 multiple insertion/delection genetic markers and comprise 63 DNA segments marked by fluorescein. The kit provided by the utility model provides a new technological way for parental right identification and individual identification in forensic medicine.

The invention belongs to the technical field of biology, and particularly relates to a molecular marker detection method, and particularly relates to a method for detecting molecular markers based ona KASP technology. The molecular markers are non-SNP and InDels. The method comprises the steps that KASP primers are designed in marker amplification target DNA sequences; for a co-dominant marker, three primers need to be designed for two target DNA sequences, and the three primers comprise two site specific primers and one common primer; for a dominant marker, two primers need to be designed for only one target DNA sequence, and the two primers comprise one site specific primer and one common primer; and the 3' end of each site specific primer is an SNP site, the Tm value of the primers is1 degree or above higher than that of a KASP joint sequence, and the size of an amplification fragment is 170 +/-130bp. According to the method, the detection range of the KASP technology is widened,and STS or SCAR is quickly detected.

The invention relates to a gallinaceous visfatin gene 9bp indel polymorphism detection method and application thereof. The detection method comprises the following steps of: designing a primer in the position of a gallinaceous visfatin gene 9bp indel locus; then carrying out PCR amplification; and detecting the polymorphism of the gene 9bp indel by the polyacrylamide gel electrophoresis, wherein the gene is of a DD genotype when 9bp is deleted, the gene is of an II genotype when 9bp is inserted, and the heterozygous individual at the locus is of an ID genotype. The correlation analysis combining the detection results and economic characters of chickens is used for the assistant selection and the molecular breeding of chickens. The detection method does not need be subject to the enzyme digestion, thereby saving the cost and the time; the detection is carried out by adopting the polyacrylamide gel electrophoresis, thereby overcoming the limitation of the agarose electrophoresis to the small-fragment nucleotide difference detection. The method has the advantages of high resolution, high detection sensitivity and accurate genotype judgment; and the gel has on requirements for the staining method, and the ethidium bromide (EB) staining and the silver staining are both applicable.