Molecular marker interlocked with pepper cucumber mosaic virus disease resistance gene qcmv-2-1 and application of molecular marker

Abstract

The invention discloses a molecular marker interlocked with a pepper cucumber mosaic virus disease resistance gene qcmv-2-1 and an application of the molecular marker. According to the molecular marker interlocked with the pepper cucumber mosaic virus disease resistance gene qcmv-2-1, pepper genome DNA is amplified through a primer pair InDel-2-134F / InDel-2-134R, and an obtained 230bp amplified fragment is the molecular marker interlocked with the pepper cucumber mosaic virus disease resistance gene qcmv-2-1; the InDel-2-134F sequence is shown as SEQ ID NO.1, and the InDel-2-134R sequence is shown as SEQ ID NO.2. According to the Indel molecular marker and corresponding primers interlocked with the qcmv-2-1 resistance gene and screened through the InDel marker and QTL-Seq technology, marker bands are simple, and the molecular marker and the application of the molecular marker can be directly used for marker-assisted section.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and relates to a molecular marker linked with pepper anti-cucumber mosaic virus disease gene qcmv-2-1 and an application thereof. Background technique [0002] Cucumber mosaic virus (CMV), a typical member of the genus Cucumovirus in the family Bromoviridae, can cause severe systemic symptoms in peppers, resulting in leaf yellowing, distortion, severe mosaic , damage the commercial properties of the fruit. Cucumber mosaic virus disease can generally lead to 20% to 30% reduction in pepper production, and can reach 50% to 60% in severe cases, and even crop failure in some areas. It is one of the most serious diseases of pepper. The detection rate of CMV in pepper production in my country is the highest, and it is the main target of pepper disease resistance breeding. [0003] In the traditional breeding method, the selection of disease-resistant materials is through inoculation identification, a...

AI Technical Summary

Problems solved by technology

The use of traditional breeding methods to break bad linkages requires a large population, long cycles, and low efficiency. Molecular marker-assisted selection (MAS) breeding can effectively overcome the shortcomings of traditional breeding and accelerate the breeding process.

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