269 results about "Mosaic virus" patented technology

The invention discloses application of a small molecule compound in prevention and treatment of plant RNA virus infection, and belongs to the technical field of biology. According to the invention, 2 '-amino-2'-deoxyguanosine (ADG) is used as a unique antiviral active component to be applied to prevention and treatment of plant RNA viruses for the first time, and broad-spectrum inhibition of various plant RNA viruses such as turnip mosaic virus (TuMV), tomato mosaic virus (ToMV) and tobacco mosaic virus (TMV) can be realized only by a low concentration of 250 [mu] M and a simple exogenous spraying mode; and after spraying, the normal growth of plants is not influenced, the risk of phytotoxicity is thoroughly avoided, the safety, high efficiency and broad spectrum of plant RNA virus prevention and control are remarkably improved, and important practical application significance is achieved for guaranteeing the yield and quality of crops in agricultural production and promoting agricultural green transformation.

The invention discloses an application of a soybean nucleolus GTP binding protein gene GmNSN1 in soybean resistance to soybean mosaic virus diseases. The invention relates to an application of a soybean nucleolus GTP binding protein gene GmNSN1 as shown in SEQ ID NO.1 and SEQ ID NO.2 in genetic engineering modification of soybean mosaic virus resistance. The soybean nucleolus GTP binding protein gene GmNSN1 can negatively regulate and control the resistance of soybeans to diseased soybean mosaic virus diseases, and the antiviral response of the soybeans can be activated due to function deficiency of the soybean nucleolus GTP binding protein gene GmNSN1. The gene is knocked out through a CRISPR / Cas9 gene editing technology, so that the resistance of soybeans to the soybean mosaic virus disease can be remarkably improved.

The invention discloses an application of a soybean ARGONAUTE family gene GmAGO5 in soybean resistance to soybean mosaic virus diseases. The invention also discloses application of the soybean ARGONAUTE family gene GmAGO5 as shown in SEQ ID NO.1 and SEQ ID NO.2 in genetic engineering modification of soybean mosaic virus resistance. The soybean ARGONAUTE family gene GmAGO5 can negatively regulate and control the resistance of soybeans to diseased soybean mosaic virus diseases, and the antiviral response of the soybeans can be activated due to function deficiency of the soybean ARGONAUTE family gene GmAGO5. The gene is knocked out through a CRISPR / Cas9 gene editing technology, so that the resistance of soybeans to the soybean mosaic virus disease can be remarkably improved.

The invention relates to a heterocyclic pyrimidinedione compound and application thereof in preparation of pesticides and medicines, in particular to the heterocyclic pyrimidinedione compound and application thereof in preparation of herbicides and plant virus resisting agents, and application in preparation of medicines for resisting fungal infection, especially candida auricula infection. The compound provided by the invention has good inhibition effect on KARI enzymes from different sources. Under the dosage of 100g / mu, the weeding effect of the compound provided by the invention on rape during stem and leaf treatment can reach 100%. The compound disclosed by the invention has a good inhibition effect on a candida auricula 12766 strain, and the minimum inhibition concentration (MIC) value of the compound can reach 0.31 mg / L when a YNB culture medium is used. The in-vivo passivation effect of the compound on the tobacco mosaic virus TMV under the concentration of 500 mg / L can reach 64.2%.

The invention provides an application of a phosphatidylinositol monophosphate 5-kinase gene BnPIP5K9 of rape in regulating and controlling the oil content of the rape. The genetic basis of the oil content of rape seeds is analyzed on the basis of multiple omics, and it is found that the expression quantity of the phosphatidylinositol monophosphate 5-kinase gene BnPIP5K9 in rape is significantly positively correlated with the oil content; the gene BnPIP5K9 is subjected to gene overexpression and knockout to create a mutant for functional verification by further utilizing a tobacco mosaic virus double-35S promoter and a CRISPR / Cas9 gene editing technology, the result shows that the oil content of rape seeds can be remarkably increased by 2.16-2.77% by overexpression of the gene, in addition, rape germplasm resources with gene BnPIP5K9 function deficiency can be obtained through the CRISPR / Cas9 technology, and the rape seed quality is improved. A new theory and a new gene resource are provided for high-oil breeding and oil improvement of the rape, and the method has extremely high application value and potential.

The invention discloses an application of a soybean GmPT1 coding gene in resisting soybean mosaic virus diseases. The invention discloses application of the soybean inorganic phosphate transporter gene GmPT1 as shown in SEQ ID NO.1 and SEQ ID NO.2 in genetic engineering modification of soybean mosaic virus resistance. The over-expression of the gene can significantly improve the resistance of soybeans to soybean mosaic virus diseases. The soybean inorganic phosphate transporter gene GmPT1 disclosed by the invention can be transformed into soybeans through genetic engineering, and the resistance of the soybeans to soybean mosaic virus diseases is finally regulated and controlled.

The invention belongs to the technical field of viral nucleic acid molecular diagnosis, and particularly relates to a kit for detecting cucumber green mottle mosaic virus based on enzyme-mediated dual-amplification nucleic acid amplification, a detection method and application. The detection system comprises a primer pair DNA upstream primer (SEQ ID NO.2) and a DNA downstream primer (SEQ ID NO.3) for amplifying a coat protein gene of the cucumber green mottle mosaic virus, and a probe (RNA primer (SEQ ID NO.1)) for detecting a CP gene, a nucleic acid amplification group enzyme system, a signal amplification group enzyme system, a nucleic acid scavenging enzyme, an activating enzyme NTP, a buffer solution and a freeze-drying protective agent, wherein the system is prepared into pre-subpackaged freeze-dried balls. The kit provided by the invention can realize RNA detection of aM concentration, and can realize early screening of cucumber green mottle mosaic virus due to high detection sensitivity.

The invention belongs to the technical field of biosensors, and provides a tobacco mosaic virus (TMV) dual-mode biosensor as well as a preparation method and application thereof. The biosensor is composed of a dual-mode luminescent material Co / Ce-TCBPE (at) Ru (dcbpy) 32-COOH, a lateral flow chromatography LFIA flexible electrode, a TMV antibody Ab1, a TMV antibody Ab1 and a self-assembled micro electrochemical luminescence (ECL) sensor, and through qualitative and quantitative dual-signal output of vision and ECL detection, the TMV detection sensitivity and accuracy are remarkably improved. The detection range of the sensor is 0.1 pg / mL-100 ng / mL, the detection limit reaches 48.9 fg / mL, and the sensor has the advantages of simplicity and convenience in operation, quick response, high stability, dual signal guarantee and the like, is suitable for quick visual detection of plant diseases, and has a wide application prospect in agricultural detection.

The invention discloses a plant endogenous small molecule polypeptide and application thereof in inhibiting RNA virus infected plants, the sequence of the small molecule polypeptide is Pep1-3: IIGVSNFDPVKRS, and the small molecule polypeptide is derived from nicotiana benthamiana; the invention relates to application of plant endogenous small molecule polypeptide in inhibiting RNA virus infection of plants. The plant is tobacco; the RNA viruses comprise tobacco mosaic viruses, tomato mosaic viruses and turnip mosaic viruses, and the small peptide Pep1-3 serves as a novel plant immunity inducer and shows the potential of the small peptide Pep1-3 in the aspect of preventing and controlling the RNA viruses. In agricultural production, prevention and treatment of virus diseases by adopting oligopeptide substances are a green and sustainable direction, and compared with traditional pesticides and plant immunity inducers, the composition has the advantages of broad spectrum, small toxicity, no drug resistance risk and long lasting period, and can generate a synergistic or synergistic effect when being mixed with other medicaments.

The invention relates to a plant source biological control agent for controlling cucumber mosaic virus and application thereof. The plant source biological control agent is one or a combination of any two or three of garlic, wedelia chinensis and mikania micrantha. Researches show that the combination of one, any two or three of garlic, wedelia chinensis and mikania micrantha has a remarkable inhibition effect on cucumber mosaic virus, and the combined use effect of garlic, wedelia chinensis and mikania micrantha is remarkably improved. According to the invention, more safe and efficient plant source control agents are provided for controlling plant diseases caused by cucumber mosaic virus, and a foundation is laid for subsequent research and development of novel cucumber mosaic virus resistant plant source pesticides. Moreover, the garlic, the wedelia chinensis and the mikania micrantha are wide in source and low in cost, and have very good application value.

The present invention discloses the use of betulinic acid in the preparation of an anti-Tubulic Mosaic Virus preparation, relating to the technical field of agricultural plant protection. The present invention used 10 mg / L of betulinic acid to continuously root-irrigate Nicotiana benthamiana plants for 5 days, and found that betulinic acid treatment significantly inhibited TuMV infection and reduced viral accumulation levels compared to the control group. Betulinic acid, as a plant-derived substance, has its own degradation pathway and has the advantages of being safe, economical, and pollution-free, meeting the requirements of sustainable agricultural development and green environmental protection. The present invention makes it possible to use betulinic acid for the prevention and treatment of plant viruses, providing a new approach for the prevention and treatment of plant viruses.

The application provides a plasmid containing modified satellite RNA and a construction method and application, and belongs to the field of plant virology and molecular biology. Each copy number of the modified satellite RNA is composed of a modified satRNA TA-Tb fragment and an HDV ribozyme fragment, and the copy number of the modified satellite RNA in the plasmid is 10. The nucleotide sequence of the modified satellite RNA is shown in SEQ No. 3. After the plasmid of the application is inoculated by agrobacterium injection, the symptoms caused by cucumber mosaic virus on different strains and the same strain (CMV Fny ) on different hosts can be significantly reduced. An effective control measure for field control of cucumber mosaic virus is provided.

This invention provides a method for creating a transgenic papaya strain highly resistant to papaya ringspot virus and papaya mosaic virus. The method involves using PRSV and PLDMV of papaya... CP The fusion gene fragment expression cassette and the selection marker gene expression cassette were placed in two separate T-DNA domains and inserted into a plant expression vector. This vector was then introduced into papaya to obtain a papaya line without the selection marker; alternatively, PRSV and PLDMV were used. CP A fusion gene expression cassette and its reverse sequence were tandemly inserted into a plant expression vector, which was then introduced into papaya to obtain... CP A papaya strain with inversely linked gene expression cassettes. The marker-free papaya of this invention does not contain selection markers, effectively improving safety; CP Papaya with inversely linked gene expression cassettes does not require further homozygosity to enhance disease resistance.

The invention discloses an application of a cordyceps militaris germ extract in preparation of an anti-tobacco mosaic virus pesticide, and belongs to the technical field of pesticides. The cordyceps militaris embryo extract has the effect of inhibiting activity on TMV, provides a new way for efficient utilization of cordyceps militaris embryos, promotes high-quality development of the cordyceps militaris cultivation industry, and has important production value and practical significance.

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group, a kit and a method for detecting four quarantine viruses and application of the LAMP primer group. Belongs to the technical field of molecular biology and plant quarantine. Aiming at conserved gene regions of bean pod mottle virus, tobacco ringspot virus, tomato ringspot virus and southern bean mosaic virus, a specific target sequence is screened through bioinformatics analysis, an LAMP primer group is designed, and the nucleotide sequence of the LAMP primer group is shown as SEQ ID NO.1-22. Furthermore, the screened primer group is combined with a micro-fluidic chip technology to construct a detection kit capable of synchronously detecting the four viruses, so that the specific identification of the four target viruses is realized. The kit has the advantages of rapidness, sensitivity, specificity, simplicity and convenience in operation and the like, the sensitivity can reach 10 <-10 > Copies / mu L, and the kit is suitable for high-throughput rapid screening of quarantine viruses in imported soybeans on port sites and has important significance in guaranteeing national biosafety.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to soybean mosaic virus resistance, an amplification primer and application of the SNP molecular marker in soybean breeding. The SNP molecular marker is located at the 910489bp site of a No.7 chromosome of a soybean genome, and the polymorphism of the SNP molecular marker is C / A. The invention provides application of the SNP molecular marker related to the soybean mosaic virus resistance in identification or auxiliary identification of the soybean mosaic virus resistance. By providing the accurate, simple, convenient and low-cost SNP molecular marker and the detection method thereof, early-stage, rapid and efficient molecular-assisted selection of soybean mosaic virus resistance characters is realized, so that the breeding process of a new variety of disease-resistant soybeans can be remarkably accelerated, and the SNP molecular marker has important breeding application value.

The invention discloses an isatis root compound preparation and a method for preventing and controlling tomato mosaic virus, and relates to the technical field of agricultural planting, the isatis root compound preparation is prepared from the following raw materials in parts by weight: 15-20 parts of isatis root, 15-20 parts of radix sophorae flavescentis, 10-15 parts of scutellaria baicalensis, 10-15 parts of szechwan chinaberry fruit, 8-12 parts of radix bupleuri, 8-12 parts of rheum officinale, 8-12 parts of astragalus membranaceus, 8-12 parts of radix stemonae, 5-8 parts of fructus forsythiae and 5-8 parts of saponin. The preparation method comprises the following steps: weighing the traditional Chinese medicine decoction pieces, fully mixing and crushing; adding clear water according to a mass ratio, uniformly stirring, heating and decocting; cooling, and adding a microbial agent for fermentation; according to the method, the traditional Chinese medicine compound preparation with the radix isatidis and the radix sophorae flavescentis as monarch drugs is adopted, the tomato mosaic virus is prevented and controlled in a green mode, the occurrence of virus diseases in the tomato growth period can be effectively prevented and controlled, and chemical pesticides are reduced or not used. And the traditional Chinese medicines are used for replacing chemical pesticides, so that the method is green and safe, no chemical pesticide is used in the whole tomato production process, and real zero pesticide residue is realized.

The invention discloses a botanical alkaloid and trichoderma synergistic preparation and application thereof, and belongs to the technical field of biological control. The invention provides a preparation containing trichoderma haworthia and chelerythrine in order to solve the technical problems of long period, low prevention and treatment efficiency and easiness in causing harm to environment and human health in a tobacco mosaic disease prevention and treatment method in the prior art. According to the preparation provided by the invention, under the condition that the dilution multiple is 50, the control effect on the tobacco mosaic virus is improved through multi-mechanism synergism, the provided preparation can be applied to tobacco planting production, the control efficiency of the tobacco mosaic disease can be effectively improved, and the economic benefit of tobacco planting is improved.

GsRPP13L The gene, its recombinant vector, recombinant bacteria and application belong to the technical field of plant disease resistance. In order to solve the technical problems that the utilization of SMV resistance genes and the analysis of disease resistance mechanism are not comprehensive enough in the prior art, a broad-spectrum and durable SMV-resistant soybean variety cannot be quickly and efficiently bred, and the resistance variety is prone to "resistance failure", a soybean gene for improving resistance to soybean mosaic virus is provided GsRPP13L The gene is shown in the nucleotide sequence SEQ ID NO.1, and the gene can improve the resistance of tobacco or soybean to soybean mosaic virus through experiments, and the present application provides a theoretical basis for plant resistance to soybean mosaic virus.

The invention discloses an application of NtTLP26 protein and a coding gene thereof in drought resistance and tobacco mosaic virus resistance of tobacco. The NtTLP26 protein is a protein as shown in A1) or A2): A1) a protein composed of an amino acid sequence as shown in SEQ ID No.2; a2) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence as shown in SEQ ID No.2, is related to drought resistance and tobacco mosaic virus resistance of tobacco and is derived from A1). The coding gene of the gene has a nucleotide sequence as shown in SEQ ID No. 1. The NtTLP26 protein disclosed by the invention is mainly used for negatively regulating the drought resistance of the tobacco and positively regulating the tobacco mosaic disease resistance of the tobacco. The gene provided by the invention has very high application value in inducing drought resistance and tobacco mosaic disease resistance of plants.

The application discloses a benzotriazole compound, a preparation method and application thereof, and belongs to the technical field of medicinal chemistry. The compound has structures as shown in general formula (I), (II) and (III). A fragment containing an amide bond substituted amino acid ester or amino acid structure is introduced into the system based on benzotriazole to synthesize a series of benzotriazole compounds containing the amino acid ester or amino acid structure. The compound has a good inhibiting effect on plant pathogenic viruses, and especially has a good inhibiting effect on plant pathogenic viruses such as tobacco mosaic virus.

The application belongs to the technical field of genetic engineering, and particularly relates to application of cassava common mosaic virus TGBp1 in inhibiting RNA silencing. The application research finds that TGBp1 has a silencing inhibition function, can inhibit mRNA of GFP from being degraded, and thus improves the expression amount of GFP protein; it is also found that TGBp1 can inhibit a target gene MeGRXC3 from being silenced and improve the expression function thereof; in addition, it is also found that TGBp1 can improve the number of mRNA degradation pathway processing bodies (a complex formed by interaction of Dcp1 protein and Dcp2 protein) of Arabidopsis. The research result not only lays a foundation for elucidating the function of TGBp1, but also provides a new target gene for cassava disease resistance breeding.

This invention discloses a Panax notoginseng ethylene response factor gene. PnERF120 Its nucleotide sequence is shown in SEQ ID NO:1, and the encoded amino acid sequence is shown in SEQ ID NO:2. PnERF120 The gene originates from Panax notoginseng, and the encoded protein sequence contains a typical conserved domain of the ERF transcription factor. This invention demonstrates, through gene cloning, bioinformatics analysis, expression pattern analysis, subcellular localization, overexpression, and other techniques and methods, that... PnERF120 This invention can be used to improve the resistance of Panax notoginseng to Panax notoginseng A virus, and also to improve the resistance of tobacco to tobacco mosaic virus. This invention provides new functional genes and application pathways for antiviral breeding and disease-resistant materials of Panax notoginseng and other plants.

The present invention relates to a hybrid Solanum lycopersicum plant comprising in its genome at least one copy of the Rug-1 resistance gene, wherein said Rug-1 resistance gene is capable of conferring a Tomato Brown Rugose Fruit Virus (ToBRFV) tolerance / resistance phenotype. The present invention further relates to a seed produced by the plant according to the present invention, a seed from which a plant according to present invention can be grown, a fruit produced by a plant according to the present invention and a part of a plant according to the present invention. The present invention further relates to a method of identifying and / or selecting a plant or plant part according to the present invention. The present invention further relates to a method for producing a Solanum lycopersicum plant having a ToBRFV tolerance / resistance phenotype according to the present invention. The present invention further relates to a method for enhancing the ToBRFV tolerance / resistance phenotype of a Solanum lycopersicum plant, the use of the Rug-1 resistance gene according to the present invention for enhancing the ToBRFV tolerance / resistance phenotype in a Solanum lycopersicum plant and the use of a genetic marker specific of the Rug-1 resistance gene according to the present invention for selecting a Solanum lycopersicum plant having an enhanced ToBRFV tolerance / resistance phenotype.

This invention relates to a method for preparing a class of substituted phenylpyrimidine guanidine compounds and their application as antiviral agents and fungicides, belonging to the field of agrochemicals. The substituted phenylpyrimidine guanidine compounds have the structure shown in general formula (I), wherein R... 1 = Monosubstituted methyl, isopropyl, methoxy, halogen; R 2 = Mono- or poly-substituted H, methyl, trifluoromethoxy, trifluoromethyl, halogen. The substituted phenylpyrimidine guanidine compounds provided by this invention have good inhibitory effects on tobacco mosaic virus in different crops and can be used for the control of this disease. The substituted phenylpyrimidine guanidine compounds provided by this invention have inhibitory effects on fungal diseases such as tomato gray mold, rice sheath blight, rice blast, and pepper blight pathogens and can be used for the control of these diseases.

The invention discloses an application of a soybean GmHB6 protein or an HB6 gene for coding the protein in antivirus of plants. According to the invention, the GmHB6 gene is introduced into a target plant through an agrobacterium transformation method, so that a soybean GmHB6 gene overexpression plant is obtained. The soybean GmHB6 overexpression plant obtained by the method disclosed by the invention can obviously reduce the infection of soybean mosaic virus.

A method of producing a transformed cell or plant body of maize of the present invention includes overexpressing, in a cell or plant body of maize, 1) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 2, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 2, the polypeptide having a function of promoting cell division of maize, or 2) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 15, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 15, the polypeptide having a function of promoting cell division of maize, the overexpressing being controlled by a promoter including a 35S promoter of Cauliflower mosaic virus.

The application discloses application of a soybean GmCLO1 coding gene in regulating resistance of soybean to soybean mosaic virus disease. The application of a soybean GmCLO1 gene shown in SEQ ID NO. 1 in genetically engineering soybean mosaic virus disease resistance. Overexpression of the gene can significantly improve the resistance of soybean to soybean mosaic virus disease, and gene editing knockout of the gene can significantly reduce the resistance of soybean to soybean mosaic virus disease. The soybean coding gene GmCLO1 disclosed by the application can be transformed into soybean through genetic engineering, and finally regulate the resistance of soybean to soybean mosaic virus disease.

The invention discloses a method for photocatalytic synthesis of a 2, 3-dihydrobenzopyran-4-ketone compound and application of the 2, 3-dihydrobenzopyran-4-ketone compound, a triplet excitation state with a double-free-radical characteristic is formed after photoexcitation of a chromone compound, and the triplet excitation state can be subjected to hydrogen atom transfer with various substrates such as ethers, esters, alcohols, alkanes, sulfides, amines and aryl phosphine compounds, so that the 2, 3-dihydrobenzopyran-4-ketone compound is obtained. And carrying out Giese addition on the generated free radicals and an unexcited chromone compound to obtain a target product. Further biological activity evaluation shows that part of products obtained in the invention show good inhibitory activity on tobacco mosaic viruses, and the good protective activities of target compounds 3i and 4b are 67.77% and 63.63% respectively when the concentration is 500mg / L, which are equivalent to 63.83% of a control drug ningnanmycin. Therefore, the compound not only is green and efficient in synthesis method, but also has potential application value in the aspects of agricultural disease control and structure protection research.

Synthetic turnip yellow mosaic virus satellite RNA molecules and satellite particles containing the same are disclosed. Synthetic turnip yellow mosaic virus satellite RNA molecules containing an internal heterologous RNA virus (HRV) amplicon are also disclosed. Also disclosed are methods of altering plant phenotypes, improving stress resistance in plants, and improving pest and pathogen resistance in plants using turnip yellow mosaic virus satellite RNA molecules and satellite particles containing the same.