881 results about "Mosaic virus" patented technology

Aspects of the invention provide modified virus-like particles that are designed for therapeutic applications. In particular, aspects of the invention provide CCMV coat proteins that are modified to generate virus-like particles, including mosaic virus-like particles, that can package and/or deliver one or more diagnostic and/or therapeutic agents. The invention also provides methods for treating subjects with one or more modified virus-like particles.

The invention provides a transcription factor TaMYB3 of a MYB class, which is separated from the plateau 115 of a purple kernel wheat variety and is used for regulating the synthetization and the metabolization of anthocyanin. TaMYB3 is positioned between 0.62 and 0.95 of a long arm physical map of wheat 4B chromosome. The subcellular fraction of a protein product is positioned on a cell nucleus. Shown by a derived amino acid sequence, the TaMYB3 codes a MYB class transcription factor regulating the synthetization and the metabolization of anthocyanin. The expression quantity of the TaMYB3 in the plateau 115 of a purple kernel wheat variety increases under the induction of light. The TaMYB3 can promote skin cells of the plateau 115 kernels after dark treatment to synthetize anthocyanin under the existence of transcription factors ZmR of corns bHLH during transient expression. Single TaMYB3 genes and single ZmR genes can not induct the synthetization of anthocyani, which shows that the TaMYB3 genes have the transcriptional activity of regulating the synthetization and the metabolization of anthocyanin. barly strip mosaic virus (BSMV) mediates the expression quantity decrease of the TaMYB3 in the plateau 115 kernels, and reduces the quantity of anthocyanin in kernels. This shows that the TaMYB3 participates in the biosynthesis of anthocyanin in the plateau 115 kernels.

The invention relates to a ferulic acid shown in a general formula (I) and the application of derivatives thereof to pesticides. When used as novel anti-phytoviral agents, the derivatives can well inhibit tobacco mosaic viruses, pepper viruses, tomato viruses, sweet potato viruses, potato viruses, melon viruses, maize dwarf mosaic viruses and the like, and effectively prevent and control the virus diseases of various crops such as tobacco, peppers, tomatoes, melons, grains, vegetables, beans and the like, and are particularly suitable for preventing and controlling tobacco mosaic. Meanings of each group are shown in specifications.

The invention provides a pesticide composition, and in particular relates to an active pesticide composition, compounding one or more of toxic fluoride phosphate, the weight ratio of toxic fluoride phosphate to other active substances is 30:0.15 to 3.3:30, and the content of effective components by weight is 1%-90%. The compound composition can be processed into water dispersible powder, suspending agent, microemulsion, aqueous emulsion, water dispersible granule, dry suspension emulsion and seed coating agent and the like, after accessory ingredient or carrying agent is added. The composition can be used to prevent and treat virus diseases caused by rice stripe virus, rice dwarf virus, maze rough dwarf virus, rice black-streaked dwarf virus, tomato virus, cucumber mosaic virus and tobacco mosaic virus, and has the beneficial effects of enhanced effect, concurrent treatment, postponed pesticide resistance, decreased dosage of toxic fluoride phosphate and reduced cost.

The invention provides a formulation composite comprising toxic fluoride phosphate and at least one growth regulator. According to the weight part, the toxic fluoride phosphate accounts for 10%-80% of the formulation composite, the growth regulator accounts for 0.0001%-2% of the formulation composite, the formulation composite also comprises an assistant, an surface active agent, a solvent and a carrier accepted by agriculture, and the formulation composite can be prepared into wettable powder, suspending agent, missible oil, microemulsion, emulsion in water, water dispersible granule, dry suspending agent, seed coating agent and other agents. The formulation composite can be used for preventing and curing tobacco mosaic virus, cucumber mosaic virus, and various virus diseases caused by viruses on rice, such as tobacco mosaic, cucumber mosaic, tomato virus disease, pepper virus disease, rice virus disease and the like and achieves the effects of improving efficacy, treating in a compatible manner, delaying medicine resistance, reducing the using amount of toxic fluoride phosphate and the like.

The invention discloses an RT-LAMP (loop-mediated isothermal amplification) rapid detection kit for cucumber green mottle mosaic virus (CGMMV) and a detection method. The method comprises the steps of extracting plant viruses RNA, detecting an RT-LAMP reaction and electrophoresis detection or fluorochrome, and determining whether a plant carries the viruses. An RT-LAMP primer is designed according to a coat protein gene conserved region of CGMMV, and an RT-LAMP technology is adopted to establish a detection technology which can rapidly and sensitively detect the cucurbitaceous plant CGMMV such as cucumbers, watermelons and calabashes with high specificity and an application method thereof in diagnosing disease. The detection method provided by the invention can utilize the rapid extraction RNA and conventional RNA extraction as an RNA template for an RT-LAMP experiment. The method is utilized to rapidly identify a CGMMV plant sample, the targeted quarantine inspection protective measure is adopted, and the loss caused by the disease is reduced.

The invention discloses a selective breeding method of a high-resistance tobacco variety and belongs to the technical field of resistive breeding of tobacco. According to the selective breeding method, the tobacco high-yield varieties Yunyan 85, Yunyan 87 and Zhongyan 100 or K326 are taken as recurrent parents, and the cold-resistant variety Honghua Dajinyuan, the drought-resistant variety Zhongyan 14, the brown spot and blackleg resistant variety Zhongyan 86, the root-knot nematode-resistant variety TI1706, the common mosaic virus-resistant variety Taiyan No.7 and the tobacco bacterial wilt-resistant variety D101 as resistant resource parents, and the seven major drought, cold and disease resistant resource genes of the tobacco are pyramided and inserted into the good-quality high-yield tobacco variety with the same genetic background by use of a synchronous backcross and convergent cross method, so that a new high-resistance, high-yield and good-quality tobacco variety, which is highly resistant to 7 drought, cold and disease damage factors and highly consistent with the original recurrent parents in other agricultural and yield traits, is obtained.

The invention relates to a method for detecting tobacco viruses by a reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) technology, which comprises the steps of tobacco virus total ribonucleic acid (RNA) extraction, RT-LAMP reaction, electrophoresis detection and virus type determination, wherein tobacco viruses are selected from two or more than two kinds of viruses from cucumber mosaic viruses (CMV), potato viruses Y (PVY), tobacco etch viruses (TEV), tobacco mosaic viruses (TMV) and tobacco vein banding mosaic viruses (TVBMV). LAMP primers are designed according to a case protein gene conserved region of the tobacco viruses, and a fast, sensitive and high-specificity method is built for detecting various main tobacco viruses by the one-step RT-LAMP technology. A newmeasure is provided for the fast detection of the tobacco viruses.

The invention discloses a myricetin derivative containing amide thioether thiadiazole, of which a general formula is represented as follows, wherein R is hydrocarbyl groups, phenyl groups, substituted phenyl groups, heteroaromatic ring groups or substituted heteroaromatic ring groups. The compound has high treatment and prevention effect against tobacco mosaic virus (TMV), has high anti-plant virus activity and can be used for preparing anti-plant virus pesticides. The compound has high inhibition activity on Xanthomonas axonopodis and Xanthomonas oryzae and can be used for preparing agricultural bactericides.

The invention discloses a Harpin coding gene (i) hrpZPsgS1(/i) and an application of a harpinZPsgS1 protein expressed by the Harpin coding gene (i) hrpZPsgS1(/i). If the obtained harpinZPsgS1 protein is used for treating tobacco plants, the growing of the tobacco plants is promoted; and the resistance of the tobacco plants against tobacco mosaic viruses and tobacco phytophthora diseases is improved obviously, wherein the resistance is not only limited in parts treated by the harpinZPsgS1 protein but also acts on the peripheral leaves and the whole plants, and the expression of PR (pathogenesis related) protein is carried out in the treating process, so that the hrpZPsgS1(/i) can induct the plant systems to obtain disease resistance. The result shows that the hrpZPsgS1 has the application value of being developed into a biogenic pesticide.

The invention discloses a coumarin compound and a preparation method and application thereof. The coumarin compound is obtained by being separated from tobacco rhizome, the molecular formula of the coumarin compound is C17H14O5, and the structure of the coumarin compound is as shown in the specification. The preparation method of the coumarin compound comprises the following steps: smashing a tobacco rhizome sample, then ultrasonically extracting for 3-5 times by using 90%-99% ethanol; merging extracting solutions; filtering, carrying out reduced-pressure concentration to obtain an extractum; and initially separating the extractum by adopting silica-gel column chromatography, and then further separating by adopting high performance liquid semi-preparation chromatography to obtain the coumarin compound. The coumarin compound disclosed by the invention has the advantages of simple structure, easiness for artificial synthesis realization and better cell activity on tobacco mosaic viruses and can be used as a lead compound resisting the tobacco mosaic viruses.

The present invention relates to a Lactuca sativa L. var. longifolia Lam seed designated as 41-49 RZ, which has leaves with a hollow main vein, broad base leaves, and exhibits resistance against downy mildew (Bremia lactucae Regel), currant-lettuce aphid (Nasonovia ribisnigri), corky root (Sphingomonas suberifaciens) and lettuce mosaic virus (LMV). The present invention also relates to a Lactuca sativa L. var. longifolia Lam plant produced by growing the “41-49 RZ” seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 41-49 RZ.

The invention discloses a breeding method of a broad-resistance green soybean variety, and belongs to the technical field of soybean breeding for disease resistance. The breeding method comprises the following steps of hybridizing a high-yield and high-quality soybean variety with soybean varieties for highly resisting frogeye leaf spot, mosaic virus disease, downy mildew and cyst nematode disease; re-hybridizing filial generations two by two; evaluating and screening seed coat colors and disease resistance of multiple generations to breed the broad-resistance green soybean variety which can simultaneously resist four diseases and are excellent in other agricultural and yield characters. The variety has excellent agricultural and yield characters, and can resist the frogeye leaf spot, the mosaic virus disease, the downy mildew and the cyst nematode disease of soybean.

The invention discloses a special organic and inorganic compound pesticide fertilizer for tobacco. The special organic and inorganic compound pesticide fertilizer comprises the following components in parts by weight based on dry materials: 30-40 parts of organic active fertilizer, 12-20 parts of ammonium nitrate, 20-30 parts of calcium superphosphate, 16-32 parts of potassium nitrate, 5-10 parts of magnesium sulfate, 1-5 parts of zinc sulfate, 1-4 parts of sodium silicate, 0.1-1 part of borax, 0.02-0.1 part of ammonium molybdate, 0.5-1 part of insecticide, 0.5-1 part of bactericide and 0.5-1 part of coating agent. Compared with a conventional fertilizer, the special organic and inorganic compound pesticide fertilizer for the tobacco, produced according to the formula of the invention, has the advantages that 20-30 percent employees can be saved, the application of a pesticide is reduced, underground insects such as agrotis ypsilon and grubs, and root black rot can be controlled, a certain resistance is achieved for tobacco mosaic virus, the ratio of first-class tobacco is increased by 3.2-5.7 percent, and the output value is increased by 294.5-2512yuan/hm<2>.

Disclosed are a variety of methods for achieving enhanced expression from a target nucleotide sequence in a plant e.g. comprising the step of transiently introducing into a tissue of a plant (e.g. a leaf) a first nucleic acid comprising the target nucleotide sequence and a second nucleic acid encoding a Post Transcriptional Gene Silencing (PTGS) suppressor protein (preferably of viral or plant origin), wherein the first and second nucleic acids are comprised within a single binary vector construct, or the first and second nucleic acid sequences are comprised within a first binary vector and a second binary vector construct respectively. The plant tissue may then be harvested for the protein. Such methods can give much higher levels of gene expression than are obtainable using stable transgenes, or certain replicating vectors. Also disclosed are specific PTGS suppressor proteins: potato virus X (pvx) p25 protein; african cassava mosaic virus (acmv) AC2 protein; rice yellow mottle virus (rymv) P1 protein; tomato bushy stunt virus (tbsv) 19K protein; plus variants of these. These suppressors may be used in any PTRS context, including the enhancement of transient expression systems.

The present invention relates to a novel microorganism Bacillus amyloliquefaciens KTGB0202 and a method for controlling plant pathogens using the same. More particularly, the present invention relates to a novel microorganism Bacillus amyloliquefaciens KTGB0202 (accession number: KCTC 10564BP) bacterium which has an excellent control effect against crop powdery mildew and a broad spectrum of antifungal activity against plant pathogenic fungi and inhibits tobacco mosaic virus infection, as well as an eco-friendly bacterial culture broth for controlling powdery mildew, which contains the same. Also, the present invention relates to antifungal substance KTGB0202 AFO1 obtained by extraction and purification from the Bacillus amyloliquefaciens KTGB0202 bacteria. The inventive Bacillus amyloliquefaciens KTGB0202 bacteria have a broad spectrum of antifungal activity, and are excellent in activity continuance and used for controlling various plant pathogens, including powdery mildew.

The invention discloses a sorghum mosaic virus tri-anti sandwich enzyme-linked immuno sorbent assay (TAS-ELISA) kit which comprises positive control, negative control, a sorghum mosaic virus polyclonal antibody, a sorghum mosaic virus monoclonal antibody, an enzyme labeled antibody and other materials and drugs, wherein the sorghum mosaic virus polyclonal antibody is obtained through rabbit immune separation serum after separating and purifying a sorghum mosaic virus sugarcane separator; and the sorghum mosaic virus monoclonal antibody is obtained by mouse immune, cell fusion and clone purification. The TAS-ELISA kit prepared by the invention has high detection sensitivity, strong specificity, high repeatability and high economic usefulness.

The invention relates to the field of plant virus gene engineering and provides a construction and an application of a tobacco vein banding mosaic virus infectious clone. A genome of a tobacco vein banding mosaic virus HN39 isolate is cloned to the downstream part of a 35S promoter in a genetic recombination mode, and the infectious clone with strong infectivity is obtained. A plant expression vector can be constructed by utilizing the infectious clone and used for stably and efficiently expressing extrinsic protein in a host plant body, and the infectious clone can be also modified into a low virulent strain for protecting a plant from being infected with a high virulent strain.

The invention discloses preparation methods and biological activity of amino acid ester type compounds containing 4-(dimethoxymethyl)pyrimidine heterocycle for prevention and treatment of tobacco mosaic viruses, namely compounds as shown in the following general formula (I) and preparation methods thereof. The invention introduces amino acid ester type compounds containing 4-(dimethoxymethyl)pyrimidine heterocycle synthesized by a one-pot process under an ultrasonic condition by taking 4-(dimethoxymethyl)pyrimidine-2-amine, substituted aldehyde and malonic ester as raw materials and takes toluene as a solvent. The compounds I5 and I8 disclosed by the invention have better inhibition effects on tobacco mosaic viruses and ralstonia solanacearum. The compounds are shown in the description.