337 results about "Mosaic virus" patented technology

The invention provides an application of an AC2 gene and the like in improving the expression quantity of GFP (Green Fluorescent Protein) and / or inhibiting degradation of an mRNA (Messenger Ribonucleic Acid) attenuated substrate taking the GFP as a reporter gene. Researches find that AC2 has an mRNA decay inhibition function, can inhibit degradation of a nonsense-mediated mRNA decay substrate taking GFP as a reporter gene and degradation of an endogenous nonsense-mediated mRNA decay substrate of arabidopsis thaliana, can improve the expression quantity of mRNA of AT1G0106020, RPS6, SMG7, AT5G35490, AT5G64430, AT1G36730 and the like, can reduce the expression quantity of AT1G72450 and AT2G400, has interaction with Dcp1 and Dcp2, and can be used for inhibiting the expression quantity of mRNA of AT1G0106020, RPS6, SMG7, AT5G35490, AT5G6430, AT1G36730 and the like. The growth ability of the yeast in an auxotroph culture medium can be improved, the expression level of Dcp1 can be reduced, and a research result lays a foundation for clarification of AC2 functions.

The invention discloses application of a small molecule compound in prevention and treatment of plant RNA virus infection, and belongs to the technical field of biology. According to the invention, 2 '-amino-2'-deoxyguanosine (ADG) is used as a unique antiviral active component to be applied to prevention and treatment of plant RNA viruses for the first time, and broad-spectrum inhibition of various plant RNA viruses such as turnip mosaic virus (TuMV), tomato mosaic virus (ToMV) and tobacco mosaic virus (TMV) can be realized only by a low concentration of 250 [mu] M and a simple exogenous spraying mode; and after spraying, the normal growth of plants is not influenced, the risk of phytotoxicity is thoroughly avoided, the safety, high efficiency and broad spectrum of plant RNA virus prevention and control are remarkably improved, and important practical application significance is achieved for guaranteeing the yield and quality of crops in agricultural production and promoting agricultural green transformation.

The invention discloses an application of a soybean nucleolus GTP binding protein gene GmNSN1 in soybean resistance to soybean mosaic virus diseases. The invention relates to an application of a soybean nucleolus GTP binding protein gene GmNSN1 as shown in SEQ ID NO.1 and SEQ ID NO.2 in genetic engineering modification of soybean mosaic virus resistance. The soybean nucleolus GTP binding protein gene GmNSN1 can negatively regulate and control the resistance of soybeans to diseased soybean mosaic virus diseases, and the antiviral response of the soybeans can be activated due to function deficiency of the soybean nucleolus GTP binding protein gene GmNSN1. The gene is knocked out through a CRISPR / Cas9 gene editing technology, so that the resistance of soybeans to the soybean mosaic virus disease can be remarkably improved.

The invention discloses an application of a soybean ARGONAUTE family gene GmAGO5 in soybean resistance to soybean mosaic virus diseases. The invention also discloses application of the soybean ARGONAUTE family gene GmAGO5 as shown in SEQ ID NO.1 and SEQ ID NO.2 in genetic engineering modification of soybean mosaic virus resistance. The soybean ARGONAUTE family gene GmAGO5 can negatively regulate and control the resistance of soybeans to diseased soybean mosaic virus diseases, and the antiviral response of the soybeans can be activated due to function deficiency of the soybean ARGONAUTE family gene GmAGO5. The gene is knocked out through a CRISPR / Cas9 gene editing technology, so that the resistance of soybeans to the soybean mosaic virus disease can be remarkably improved.

The invention discloses a pCAMBIA1300-mini RNA3-BC-NLPPya vector as well as a construction method and application of the pCAMBIA1300-mini RNA3-BC-NLPPya vector in high cucumber mosaic virus resistance, the vector is characterized in that mini RNA3-BC-NLPPya is constructed to a pCAMBIA1300 vector skeleton, and the gene sequences of mini RNA3-BC and NLPPya are as shown in SEQ ID NO: 2 and SEQ ID NO: 5. The invention finds that the miniRNA3-BC-NLPPya can efficiently inhibit the infection of the CMV and effectively weaken the harm caused by the CMV, does not need to apply any medicine, is an efficient and green disease-resistant technology, and can provide a new strategy for preventing and controlling the cucumber mosaic virus and similar viruses thereof.

The invention belongs to the technical field of analytical chemistry detection, and particularly relates to a construction method and application of a tobacco mosaic virus molecular imprinting sensor based on MIL-101 (Fe). The construction method comprises the following steps: by taking a tobacco mosaic virus TMV as a template virus, preparing a molecularly imprinted polymer MIPs with a TMV specific recognition cavity on the surface of MIL-101 (Fe) through a free radical polymerization reaction; the template virus is removed in the preparation process of the molecularly imprinted polymer MIPs, and a TMV specific recognition cavity of the molecularly imprinted polymer MIPs contains MIL-101 (Fe); the molecularly imprinted polymer MIPs is the tobacco mosaic virus molecularly imprinted sensor based on MIL-101 (Fe). Hydrochloric acid is introduced, so that the TMV specific recognition cavity structure of the molecularly imprinted polymer MIPs is cracked to release Fe < 3 + >, then potassium thiocyanate is added, and the Fe < 3 + > and KSCN are subjected to a complexation reaction to generate a red complex. And the complexation reaction of Fe < 3 + > and KSCN is used as a signal probe, the rapid semi-quantitative visual detection of the target virus TMV by naked eyes is realized, and quantitative detection and analysis are carried out through an ultraviolet spectrophotometer.

The invention provides an application of a TGBp1 gene, or a TGBp1 gene coding protein, or a recombinant vector or host bacteria containing a TGBp1 gene coding region in down-regulation of the MeGRXC3 expression level. Researches find that TGBp1 and MeGRXC3 interact with each other, so that the growth ability of yeast in an SD / -Leu / -Trp / -His / -Ade auxotroph culture medium can be improved, the expression of MeGRXC3 can be down-regulated, the active oxygen content of tobacco leaves for co-expression of TGBp1 and MeGRXC3 is improved, and the growth ability of the yeast in the SD / -Leu / -Trp / -His / -Ade auxotroph culture medium is improved. Research results lay a theoretical foundation for breeding of common cassava mosaic virus disease resistance.

The invention relates to a heterocyclic pyrimidinedione compound and application thereof in preparation of pesticides and medicines, in particular to the heterocyclic pyrimidinedione compound and application thereof in preparation of herbicides and plant virus resisting agents, and application in preparation of medicines for resisting fungal infection, especially candida auricula infection. The compound provided by the invention has good inhibition effect on KARI enzymes from different sources. Under the dosage of 100g / mu, the weeding effect of the compound provided by the invention on rape during stem and leaf treatment can reach 100%. The compound disclosed by the invention has a good inhibition effect on a candida auricula 12766 strain, and the minimum inhibition concentration (MIC) value of the compound can reach 0.31 mg / L when a YNB culture medium is used. The in-vivo passivation effect of the compound on the tobacco mosaic virus TMV under the concentration of 500 mg / L can reach 64.2%.

The invention discloses a sugarcane streak mosaic virus infectious cloning vector as well as a construction method and application thereof, and belongs to the technical field of biology. The recombinant plasmid provided by the invention contains a whole genome of the SCSMV, and the nucleotide sequence of the whole genome is the 771 to 10583 nucleotides of SEQ ID No. 1. The recombinant plasmid disclosed by the invention is obtained by taking a pCB301 vector as a skeleton and then inserting the skeleton into a whole genome of SCSMV, and the recombinant agrobacterium tumefaciens (pCB301-SCSMV infectious clone) prepared by the recombinant plasmid can successfully infect nicotiana benthamiana and has efficient infection ability; the infectious clone of the recombinant expression plasmid prepared from the recombinant plasmid can successfully infect nicotiana benthamiana, has efficient infection ability and can express foreign proteins.

The invention provides a coumarin derivative containing oxadiazole (thiadiazole) thioether and a preparation method and application thereof, the structural general formula of the coumarin derivative containing oxadiazole (thiadiazole) thioether is shown as I, and the structural general formula of the coumarin derivative containing thiadiazole thioether is shown as II, the synthesis route of the coumarin derivative containing oxadiazole (thiadiazole) thioether is simple and practical, the preparation raw materials are easy to obtain, the cost is low, and the prepared compound has a good control effect on plant viruses including tobacco mosaic virus, cucumber mosaic virus, potato Y virus, southern rice black-streaked dwarf virus or rice stripe virus. According to the invention, a favorable lead compound is provided for the creation of a natural bionic antiviral agent; # imgabs0 #

The invention belongs to the technical field of plant biology, and particularly relates to wheat stem rot related protein TaNDR1 and application thereof. According to the invention, a gene with a non-microspecies specific disease resistance function is cloned from wheat and is named as TaNDR1. According to the invention, a barley stripe mosaic virus induced vector for silencing the TaNDR1 gene in wheat is constructed, the expression of the TaNDR1 gene is reduced, a silent TaNDR1 wheat plant is obtained, and after the silent TaNDR1 wheat is inoculated with stem rot pathogen, the stem rot resistance of the wheat is obviously reduced. Therefore, after the expression quantity of the TaNDR1 in the susceptible wheat plant is increased, the stem rot resistance of the susceptible wheat plant can be obviously improved, so that the gene can be used for cultivating the stem rot resistant wheat.

The invention provides an application of a phosphatidylinositol monophosphate 5-kinase gene BnPIP5K9 of rape in regulating and controlling the oil content of the rape. The genetic basis of the oil content of rape seeds is analyzed on the basis of multiple omics, and it is found that the expression quantity of the phosphatidylinositol monophosphate 5-kinase gene BnPIP5K9 in rape is significantly positively correlated with the oil content; the gene BnPIP5K9 is subjected to gene overexpression and knockout to create a mutant for functional verification by further utilizing a tobacco mosaic virus double-35S promoter and a CRISPR / Cas9 gene editing technology, the result shows that the oil content of rape seeds can be remarkably increased by 2.16-2.77% by overexpression of the gene, in addition, rape germplasm resources with gene BnPIP5K9 function deficiency can be obtained through the CRISPR / Cas9 technology, and the rape seed quality is improved. A new theory and a new gene resource are provided for high-oil breeding and oil improvement of the rape, and the method has extremely high application value and potential.

The invention belongs to the technical field of plant biology, and particularly relates to application of TaC2H2 protein in wheat stem rot resistance. According to the invention, a barley stripe mosaic virus inducible vector for silencing the TaC2H2 gene in wheat is constructed, the expression of the TaC2H2 gene is reduced, a silent TaC2H2 wheat plant is obtained, and the stem rot resistance of the silent TaC2H2 wheat is obviously reduced after the silent TaC2H2 wheat is inoculated with stem rot pathogens. Therefore, the TaC2H2 gene plays a positive role in wheat stem rot resistance regulation and control, so that the gene can be used for cultivating stem rot resistant wheat.

The invention provides an application of a CP gene, or a protein encoded by the CP gene, or a recombinant vector or host bacteria containing a CP gene coding region in down-regulation of the MeGRXC3 expression level. Researches find that CP and MeGRXC3 interact with each other, so that the growth ability of yeast in an SD / -Leu / -Trp / -His / -Ade auxotrophic culture medium can be improved, cell autophagy can be activated, the expression of MeGRXC3 can be down-regulated, and the active oxygen content of plant tissues co-expressed with MeGRXC3 can be increased. Research results lay a theoretical foundation for breeding of common cassava mosaic virus disease resistance.

The invention discloses an application of a soybean GmPT1 coding gene in resisting soybean mosaic virus diseases. The invention discloses application of the soybean inorganic phosphate transporter gene GmPT1 as shown in SEQ ID NO.1 and SEQ ID NO.2 in genetic engineering modification of soybean mosaic virus resistance. The over-expression of the gene can significantly improve the resistance of soybeans to soybean mosaic virus diseases. The soybean inorganic phosphate transporter gene GmPT1 disclosed by the invention can be transformed into soybeans through genetic engineering, and the resistance of the soybeans to soybean mosaic virus diseases is finally regulated and controlled.

The invention belongs to the technical field of viral nucleic acid molecular diagnosis, and particularly relates to a kit for detecting cucumber green mottle mosaic virus based on enzyme-mediated dual-amplification nucleic acid amplification, a detection method and application. The detection system comprises a primer pair DNA upstream primer (SEQ ID NO.2) and a DNA downstream primer (SEQ ID NO.3) for amplifying a coat protein gene of the cucumber green mottle mosaic virus, and a probe (RNA primer (SEQ ID NO.1)) for detecting a CP gene, a nucleic acid amplification group enzyme system, a signal amplification group enzyme system, a nucleic acid scavenging enzyme, an activating enzyme NTP, a buffer solution and a freeze-drying protective agent, wherein the system is prepared into pre-subpackaged freeze-dried balls. The kit provided by the invention can realize RNA detection of aM concentration, and can realize early screening of cucumber green mottle mosaic virus due to high detection sensitivity.

The invention belongs to the technical field of biosensors, and provides a tobacco mosaic virus (TMV) dual-mode biosensor as well as a preparation method and application thereof. The biosensor is composed of a dual-mode luminescent material Co / Ce-TCBPE (at) Ru (dcbpy) 32-COOH, a lateral flow chromatography LFIA flexible electrode, a TMV antibody Ab1, a TMV antibody Ab1 and a self-assembled micro electrochemical luminescence (ECL) sensor, and through qualitative and quantitative dual-signal output of vision and ECL detection, the TMV detection sensitivity and accuracy are remarkably improved. The detection range of the sensor is 0.1 pg / mL-100 ng / mL, the detection limit reaches 48.9 fg / mL, and the sensor has the advantages of simplicity and convenience in operation, quick response, high stability, dual signal guarantee and the like, is suitable for quick visual detection of plant diseases, and has a wide application prospect in agricultural detection.

The invention discloses a plant endogenous small molecule polypeptide and application thereof in inhibiting RNA virus infected plants, the sequence of the small molecule polypeptide is Pep1-3: IIGVSNFDPVKRS, and the small molecule polypeptide is derived from nicotiana benthamiana; the invention relates to application of plant endogenous small molecule polypeptide in inhibiting RNA virus infection of plants. The plant is tobacco; the RNA viruses comprise tobacco mosaic viruses, tomato mosaic viruses and turnip mosaic viruses, and the small peptide Pep1-3 serves as a novel plant immunity inducer and shows the potential of the small peptide Pep1-3 in the aspect of preventing and controlling the RNA viruses. In agricultural production, prevention and treatment of virus diseases by adopting oligopeptide substances are a green and sustainable direction, and compared with traditional pesticides and plant immunity inducers, the composition has the advantages of broad spectrum, small toxicity, no drug resistance risk and long lasting period, and can generate a synergistic or synergistic effect when being mixed with other medicaments.

The invention relates to a plant source biological control agent for controlling cucumber mosaic virus and application thereof. The plant source biological control agent is one or a combination of any two or three of garlic, wedelia chinensis and mikania micrantha. Researches show that the combination of one, any two or three of garlic, wedelia chinensis and mikania micrantha has a remarkable inhibition effect on cucumber mosaic virus, and the combined use effect of garlic, wedelia chinensis and mikania micrantha is remarkably improved. According to the invention, more safe and efficient plant source control agents are provided for controlling plant diseases caused by cucumber mosaic virus, and a foundation is laid for subsequent research and development of novel cucumber mosaic virus resistant plant source pesticides. Moreover, the garlic, the wedelia chinensis and the mikania micrantha are wide in source and low in cost, and have very good application value.

The invention provides an artificial plant bidirectional constitutive strong promoter, and particularly relates to a high-activity short transcription enhancer fragment which is formed by connecting three plant endogenous fragments and a plant virus in series and serves as a transcription activation basic element. A minimum core promoter element Mini 35S from a cauliflower mosaic virus and a minimum core promoter element CsVMV mini-pro functional module from a cassava vein mosaic virus are respectively integrated at two ends, so that the plant constitutive strong promoter with bidirectional starting capability is constructed. According to the invention, a synthetic quadruple STE transcription enhancer, a Mini 35S promoter and a CsVMV mini-pro promoter are functionally coupled to form an expression regulation tool with broad-spectrum adaptability to plant species, and two operably connected target gene expression cassettes can be simultaneously driven by a short regulation sequence to realize synergistic efficient expression in plant cells.

The invention relates to chloropyrrolo [4, 3, 2-de] quinoline alkaloids as well as a preparation method and application thereof. The structural formula of the quinoline alkaloid is shown in the specification. The obtained chloro-pyrrolo [4, 3, 2-de] quinoline alkaloids I-1-I-20 have anti-plant virus activity, and especially can inhibit tobacco mosaic virus and potato Y virus. The invention develops an efficient synthesis method of the pyrrolo [4, 3, 2-de] quinoline derivative, and lays a foundation for biological activity research of the pyrrolo [4, 3, 2-de] quinoline compound. # imgabs0 #

The present invention discloses the application of a plant APT2 protein and the encoding nucleic acid in the breeding of tobacco crops. The present invention provides the application of any one of the following substances 1-3 in regulating the resistance of tobacco plants to pathogens and regulating the height of tobacco plants: 1. the protein APT2; 2. the nucleic acid molecule encoding the protein APT2; 3. a recombinant vector, expression cassette, transgenic crop, transgenic cell line or recombinant bacterium containing the nucleic acid molecule encoding the protein APT2. The experiments of the present invention prove that APT2 can be used to regulate the height of tobacco plants and to control tobacco mosaic virus or other pathogens by affecting the efficiency of viral cell-to-cell movement.

The present invention discloses the use of betulinic acid in the preparation of an anti-Tubulic Mosaic Virus preparation, relating to the technical field of agricultural plant protection. The present invention used 10 mg / L of betulinic acid to continuously root-irrigate Nicotiana benthamiana plants for 5 days, and found that betulinic acid treatment significantly inhibited TuMV infection and reduced viral accumulation levels compared to the control group. Betulinic acid, as a plant-derived substance, has its own degradation pathway and has the advantages of being safe, economical, and pollution-free, meeting the requirements of sustainable agricultural development and green environmental protection. The present invention makes it possible to use betulinic acid for the prevention and treatment of plant viruses, providing a new approach for the prevention and treatment of plant viruses.

The application provides a plasmid containing modified satellite RNA and a construction method and application, and belongs to the field of plant virology and molecular biology. Each copy number of the modified satellite RNA is composed of a modified satRNA TA-Tb fragment and an HDV ribozyme fragment, and the copy number of the modified satellite RNA in the plasmid is 10. The nucleotide sequence of the modified satellite RNA is shown in SEQ No. 3. After the plasmid of the application is inoculated by agrobacterium injection, the symptoms caused by cucumber mosaic virus on different strains and the same strain (CMV Fny ) on different hosts can be significantly reduced. An effective control measure for field control of cucumber mosaic virus is provided.

This invention provides a method for creating a transgenic papaya strain highly resistant to papaya ringspot virus and papaya mosaic virus. The method involves using PRSV and PLDMV of papaya... CP The fusion gene fragment expression cassette and the selection marker gene expression cassette were placed in two separate T-DNA domains and inserted into a plant expression vector. This vector was then introduced into papaya to obtain a papaya line without the selection marker; alternatively, PRSV and PLDMV were used. CP A fusion gene expression cassette and its reverse sequence were tandemly inserted into a plant expression vector, which was then introduced into papaya to obtain... CP A papaya strain with inversely linked gene expression cassettes. The marker-free papaya of this invention does not contain selection markers, effectively improving safety; CP Papaya with inversely linked gene expression cassettes does not require further homozygosity to enhance disease resistance.

The invention discloses an application of a cordyceps militaris germ extract in preparation of an anti-tobacco mosaic virus pesticide, and belongs to the technical field of pesticides. The cordyceps militaris embryo extract has the effect of inhibiting activity on TMV, provides a new way for efficient utilization of cordyceps militaris embryos, promotes high-quality development of the cordyceps militaris cultivation industry, and has important production value and practical significance.

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group, a kit and a method for detecting four quarantine viruses and application of the LAMP primer group. Belongs to the technical field of molecular biology and plant quarantine. Aiming at conserved gene regions of bean pod mottle virus, tobacco ringspot virus, tomato ringspot virus and southern bean mosaic virus, a specific target sequence is screened through bioinformatics analysis, an LAMP primer group is designed, and the nucleotide sequence of the LAMP primer group is shown as SEQ ID NO.1-22. Furthermore, the screened primer group is combined with a micro-fluidic chip technology to construct a detection kit capable of synchronously detecting the four viruses, so that the specific identification of the four target viruses is realized. The kit has the advantages of rapidness, sensitivity, specificity, simplicity and convenience in operation and the like, the sensitivity can reach 10 <-10 > Copies / mu L, and the kit is suitable for high-throughput rapid screening of quarantine viruses in imported soybeans on port sites and has important significance in guaranteeing national biosafety.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to soybean mosaic virus resistance, an amplification primer and application of the SNP molecular marker in soybean breeding. The SNP molecular marker is located at the 910489bp site of a No.7 chromosome of a soybean genome, and the polymorphism of the SNP molecular marker is C / A. The invention provides application of the SNP molecular marker related to the soybean mosaic virus resistance in identification or auxiliary identification of the soybean mosaic virus resistance. By providing the accurate, simple, convenient and low-cost SNP molecular marker and the detection method thereof, early-stage, rapid and efficient molecular-assisted selection of soybean mosaic virus resistance characters is realized, so that the breeding process of a new variety of disease-resistant soybeans can be remarkably accelerated, and the SNP molecular marker has important breeding application value.

The invention discloses an isatis root compound preparation and a method for preventing and controlling tomato mosaic virus, and relates to the technical field of agricultural planting, the isatis root compound preparation is prepared from the following raw materials in parts by weight: 15-20 parts of isatis root, 15-20 parts of radix sophorae flavescentis, 10-15 parts of scutellaria baicalensis, 10-15 parts of szechwan chinaberry fruit, 8-12 parts of radix bupleuri, 8-12 parts of rheum officinale, 8-12 parts of astragalus membranaceus, 8-12 parts of radix stemonae, 5-8 parts of fructus forsythiae and 5-8 parts of saponin. The preparation method comprises the following steps: weighing the traditional Chinese medicine decoction pieces, fully mixing and crushing; adding clear water according to a mass ratio, uniformly stirring, heating and decocting; cooling, and adding a microbial agent for fermentation; according to the method, the traditional Chinese medicine compound preparation with the radix isatidis and the radix sophorae flavescentis as monarch drugs is adopted, the tomato mosaic virus is prevented and controlled in a green mode, the occurrence of virus diseases in the tomato growth period can be effectively prevented and controlled, and chemical pesticides are reduced or not used. And the traditional Chinese medicines are used for replacing chemical pesticides, so that the method is green and safe, no chemical pesticide is used in the whole tomato production process, and real zero pesticide residue is realized.

The invention provides an application of modified tobacco leaf polysaccharide in resisting tobacco mosaic virus. According to the application of the modified tobacco polysaccharide in resisting the tobacco mosaic virus, the modified tobacco polysaccharide comprises one or more of acetylated tobacco polysaccharide, carboxymethylated tobacco polysaccharide and phosphorylated tobacco polysaccharide. The modified tobacco leaf polysaccharide is used for preventing and treating tobacco mosaic viruses by improving the protection effect on tobacco plants and the passivation effect on the tobacco mosaic viruses. Tobacco polysaccharide is used as a raw material, three chemical derivatization methods of acetylation, carboxymethylation and phosphorylation are adopted for modifying the tobacco polysaccharide, active functional groups are introduced, the solubility of the modified tobacco polysaccharide is remarkably improved, and the activity of the tobacco polysaccharide for resisting tobacco mosaic viruses is remarkably improved; in-vitro polymerization of the tobacco mosaic virus coat protein is inhibited, the structure of the tobacco mosaic virus is destroyed, and a foundation is laid for application of the tobacco mosaic virus in the field of botanical pesticides.