140 results about "Mosaic virus" patented technology

The invention relates to a plant source biological control agent for controlling cucumber mosaic virus and application thereof. The plant source biological control agent is one or a combination of any two or three of garlic, wedelia chinensis and mikania micrantha. Researches show that the combination of one, any two or three of garlic, wedelia chinensis and mikania micrantha has a remarkable inhibition effect on cucumber mosaic virus, and the combined use effect of garlic, wedelia chinensis and mikania micrantha is remarkably improved. According to the invention, more safe and efficient plant source control agents are provided for controlling plant diseases caused by cucumber mosaic virus, and a foundation is laid for subsequent research and development of novel cucumber mosaic virus resistant plant source pesticides. Moreover, the garlic, the wedelia chinensis and the mikania micrantha are wide in source and low in cost, and have very good application value.

The application provides a plasmid containing modified satellite RNA and a construction method and application, and belongs to the field of plant virology and molecular biology. Each copy number of the modified satellite RNA is composed of a modified satRNA TA-Tb fragment and an HDV ribozyme fragment, and the copy number of the modified satellite RNA in the plasmid is 10. The nucleotide sequence of the modified satellite RNA is shown in SEQ No. 3. After the plasmid of the application is inoculated by agrobacterium injection, the symptoms caused by cucumber mosaic virus on different strains and the same strain (CMV Fny ) on different hosts can be significantly reduced. An effective control measure for field control of cucumber mosaic virus is provided.

This invention provides a method for creating a transgenic papaya strain highly resistant to papaya ringspot virus and papaya mosaic virus. The method involves using PRSV and PLDMV of papaya... CP The fusion gene fragment expression cassette and the selection marker gene expression cassette were placed in two separate T-DNA domains and inserted into a plant expression vector. This vector was then introduced into papaya to obtain a papaya line without the selection marker; alternatively, PRSV and PLDMV were used. CP A fusion gene expression cassette and its reverse sequence were tandemly inserted into a plant expression vector, which was then introduced into papaya to obtain... CP A papaya strain with inversely linked gene expression cassettes. The marker-free papaya of this invention does not contain selection markers, effectively improving safety; CP Papaya with inversely linked gene expression cassettes does not require further homozygosity to enhance disease resistance.

The invention discloses an application of a cordyceps militaris germ extract in preparation of an anti-tobacco mosaic virus pesticide, and belongs to the technical field of pesticides. The cordyceps militaris embryo extract has the effect of inhibiting activity on TMV, provides a new way for efficient utilization of cordyceps militaris embryos, promotes high-quality development of the cordyceps militaris cultivation industry, and has important production value and practical significance.

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group, a kit and a method for detecting four quarantine viruses and application of the LAMP primer group. Belongs to the technical field of molecular biology and plant quarantine. Aiming at conserved gene regions of bean pod mottle virus, tobacco ringspot virus, tomato ringspot virus and southern bean mosaic virus, a specific target sequence is screened through bioinformatics analysis, an LAMP primer group is designed, and the nucleotide sequence of the LAMP primer group is shown as SEQ ID NO.1-22. Furthermore, the screened primer group is combined with a micro-fluidic chip technology to construct a detection kit capable of synchronously detecting the four viruses, so that the specific identification of the four target viruses is realized. The kit has the advantages of rapidness, sensitivity, specificity, simplicity and convenience in operation and the like, the sensitivity can reach 10 <-10 > Copies / mu L, and the kit is suitable for high-throughput rapid screening of quarantine viruses in imported soybeans on port sites and has important significance in guaranteeing national biosafety.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to soybean mosaic virus resistance, an amplification primer and application of the SNP molecular marker in soybean breeding. The SNP molecular marker is located at the 910489bp site of a No.7 chromosome of a soybean genome, and the polymorphism of the SNP molecular marker is C / A. The invention provides application of the SNP molecular marker related to the soybean mosaic virus resistance in identification or auxiliary identification of the soybean mosaic virus resistance. By providing the accurate, simple, convenient and low-cost SNP molecular marker and the detection method thereof, early-stage, rapid and efficient molecular-assisted selection of soybean mosaic virus resistance characters is realized, so that the breeding process of a new variety of disease-resistant soybeans can be remarkably accelerated, and the SNP molecular marker has important breeding application value.

The invention discloses an isatis root compound preparation and a method for preventing and controlling tomato mosaic virus, and relates to the technical field of agricultural planting, the isatis root compound preparation is prepared from the following raw materials in parts by weight: 15-20 parts of isatis root, 15-20 parts of radix sophorae flavescentis, 10-15 parts of scutellaria baicalensis, 10-15 parts of szechwan chinaberry fruit, 8-12 parts of radix bupleuri, 8-12 parts of rheum officinale, 8-12 parts of astragalus membranaceus, 8-12 parts of radix stemonae, 5-8 parts of fructus forsythiae and 5-8 parts of saponin. The preparation method comprises the following steps: weighing the traditional Chinese medicine decoction pieces, fully mixing and crushing; adding clear water according to a mass ratio, uniformly stirring, heating and decocting; cooling, and adding a microbial agent for fermentation; according to the method, the traditional Chinese medicine compound preparation with the radix isatidis and the radix sophorae flavescentis as monarch drugs is adopted, the tomato mosaic virus is prevented and controlled in a green mode, the occurrence of virus diseases in the tomato growth period can be effectively prevented and controlled, and chemical pesticides are reduced or not used. And the traditional Chinese medicines are used for replacing chemical pesticides, so that the method is green and safe, no chemical pesticide is used in the whole tomato production process, and real zero pesticide residue is realized.

GsRPP13L The gene, its recombinant vector, recombinant bacteria and application belong to the technical field of plant disease resistance. In order to solve the technical problems that the utilization of SMV resistance genes and the analysis of disease resistance mechanism are not comprehensive enough in the prior art, a broad-spectrum and durable SMV-resistant soybean variety cannot be quickly and efficiently bred, and the resistance variety is prone to "resistance failure", a soybean gene for improving resistance to soybean mosaic virus is provided GsRPP13L The gene is shown in the nucleotide sequence SEQ ID NO.1, and the gene can improve the resistance of tobacco or soybean to soybean mosaic virus through experiments, and the present application provides a theoretical basis for plant resistance to soybean mosaic virus.

The application discloses a benzotriazole compound, a preparation method and application thereof, and belongs to the technical field of medicinal chemistry. The compound has structures as shown in general formula (I), (II) and (III). A fragment containing an amide bond substituted amino acid ester or amino acid structure is introduced into the system based on benzotriazole to synthesize a series of benzotriazole compounds containing the amino acid ester or amino acid structure. The compound has a good inhibiting effect on plant pathogenic viruses, and especially has a good inhibiting effect on plant pathogenic viruses such as tobacco mosaic virus.

The application belongs to the technical field of genetic engineering, and particularly relates to application of cassava common mosaic virus TGBp1 in inhibiting RNA silencing. The application research finds that TGBp1 has a silencing inhibition function, can inhibit mRNA of GFP from being degraded, and thus improves the expression amount of GFP protein; it is also found that TGBp1 can inhibit a target gene MeGRXC3 from being silenced and improve the expression function thereof; in addition, it is also found that TGBp1 can improve the number of mRNA degradation pathway processing bodies (a complex formed by interaction of Dcp1 protein and Dcp2 protein) of Arabidopsis. The research result not only lays a foundation for elucidating the function of TGBp1, but also provides a new target gene for cassava disease resistance breeding.

This invention discloses a Panax notoginseng ethylene response factor gene. PnERF120 Its nucleotide sequence is shown in SEQ ID NO:1, and the encoded amino acid sequence is shown in SEQ ID NO:2. PnERF120 The gene originates from Panax notoginseng, and the encoded protein sequence contains a typical conserved domain of the ERF transcription factor. This invention demonstrates, through gene cloning, bioinformatics analysis, expression pattern analysis, subcellular localization, overexpression, and other techniques and methods, that... PnERF120 This invention can be used to improve the resistance of Panax notoginseng to Panax notoginseng A virus, and also to improve the resistance of tobacco to tobacco mosaic virus. This invention provides new functional genes and application pathways for antiviral breeding and disease-resistant materials of Panax notoginseng and other plants.

A method of producing a transformed cell or plant body of maize of the present invention includes overexpressing, in a cell or plant body of maize, 1) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 2, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 2, the polypeptide having a function of promoting cell division of maize, or 2) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 15, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 15, the polypeptide having a function of promoting cell division of maize, the overexpressing being controlled by a promoter including a 35S promoter of Cauliflower mosaic virus.

The invention discloses a method for photocatalytic synthesis of a 2, 3-dihydrobenzopyran-4-ketone compound and application of the 2, 3-dihydrobenzopyran-4-ketone compound, a triplet excitation state with a double-free-radical characteristic is formed after photoexcitation of a chromone compound, and the triplet excitation state can be subjected to hydrogen atom transfer with various substrates such as ethers, esters, alcohols, alkanes, sulfides, amines and aryl phosphine compounds, so that the 2, 3-dihydrobenzopyran-4-ketone compound is obtained. And carrying out Giese addition on the generated free radicals and an unexcited chromone compound to obtain a target product. Further biological activity evaluation shows that part of products obtained in the invention show good inhibitory activity on tobacco mosaic viruses, and the good protective activities of target compounds 3i and 4b are 67.77% and 63.63% respectively when the concentration is 500mg / L, which are equivalent to 63.83% of a control drug ningnanmycin. Therefore, the compound not only is green and efficient in synthesis method, but also has potential application value in the aspects of agricultural disease control and structure protection research.

Synthetic turnip yellow mosaic virus satellite RNA molecules and satellite particles containing the same are disclosed. Synthetic turnip yellow mosaic virus satellite RNA molecules containing an internal heterologous RNA virus (HRV) amplicon are also disclosed. Also disclosed are methods of altering plant phenotypes, improving stress resistance in plants, and improving pest and pathogen resistance in plants using turnip yellow mosaic virus satellite RNA molecules and satellite particles containing the same.

The invention discloses application of NDUFS1 protein or a coding gene thereof in cultivation of antiviral plants. According to the invention, the NDUFS1 gene from potatoes is found to be capable of participating in infection of various viruses of potato Y viruses for the first time, and the silencing NDUFS1 can inhibit replication of the potato Y viruses and tobacco vein banding mosaic viruses; a gene editing technology is utilized, specific sgRNA is designed for nucleotide regions from the 221 site to the 242 site of a coding region of the potato StNDUFS1 gene (SEQ ID NO.3) for editing, a mutant potato plant with StNDUFS1 gene function deletion is obtained, and the plant has remarkable resistance to the potato Y virus. Therefore, the NDUFS1 protein can be used as a novel antiviral target, is used for cultivating plants resisting the potyvirus virus, and plays an important role in preventing and treating the potyvirus virus.

The application discloses a CMV virus-like particle for producing a VLP recombinant vaccine and a preparation method thereof, the CMV virus-like particle is obtained by cloning a C-terminal G4SLPETG modified plant virus cucumber mosaic virus CMV capsid gene into a prokaryotic expression vector to obtain a recombinant expression vector, the recombinant expression vector is transfected into E. coli BL21 (DE3), and expression is obtained through the recombinant E. coli BL21 (DE3); and the amino acid sequence of the C-terminal G4SLPETG modified CMV is SEQ ID NO. 1. Test proves that the recombinant strain constructed by the application is stable to expression of an exogenous protein. The recombinant protein expressed by the application is used for preparing a virus-like particle for antigen coupling, the coupling efficiency is high, the antigen purity is high, the safety is good, the virus-like particle is not pathogenic to mice and other animals, and the safety evaluation is easy.

The invention belongs to the technical field of pesticides, and relates to a phenol derivative containing a phthalide structure as well as a preparation method and application thereof. According to the invention, phthalide and phenol are organically combined and modified to synthesize the phenol derivative containing the phthalide structure. According to the invention, carboxybenzaldehyde, phthalide, phenol compounds and the like are respectively used as raw materials, the phenol derivative containing the phthalide structure is prepared through condensation, nucleophilic substitution and other reactions, the reaction conditions are mild, the used acidic reagent, alkaline reagent and Lewis acid are easy to obtain, and the product is simple and convenient to purify. The phenol derivative containing the phthalide structure has a good prevention and treatment effect on various plant viruses such as tobacco mosaic viruses, pepper viruses and wheat viruses, solves the problems that existing plant virus resisting agents are deficient in variety and insufficient in activity, and is expected to be developed into a novel plant virus resisting agent which is efficient, safe, economical and environmentally friendly.

The present invention relates to a method for detecting and / or selecting S. lycopersicum plants resistant to Tomato Brown Rugose Fruit Virus (TBRFV), which inhibit, reduce, or delay virus replication, characterized in that it comprises the steps of: a) detecting at least one of the following markers: T allele of TO-0122252 (SEQ ID NO:7), C allele of TO-0144317 (SEQ ID NO:8), T allele of TO-0142270 (SEQ ID NO:9), G allele of TO-0142294 (SEQ ID NO:10), A allele of TO-0142303 (SEQ ID NO:11), A allele of TO-0142306 (SEQ ID NO:12), G allele of TO-0182276 (SEQ ID NO:13), G allele of TO-0181040 (SEQ ID NO:14), G allele of TO-0123057 (SEQ ID NO:15), A allele of TO-0125528 (SEQ ID NO:16), C allele of TO-0162432 (SEQ ID NO:17) and T allele of TO-0162427 (SEQ ID NO:18), and b) detect the homozygous presence of the Tm-1 gene, preferably by detecting the A allele of the SNP marker TO-0200838 (SEQ ID NO:21).

The present invention relates to a plant of the S. lycopersicum species that is resistant to tobamovirus at a temperature of at least 28°C, comprising a TBRFV resistance gene and further comprising at least one temperature-reducing (TSR) gene. The present invention further relates to a combination of a TBRFV resistance gene combined with at least one temperature-reducing (TSR) gene that provides resistance to tobamovirus in S. lycopersicum plants at a temperature of at least 28°C, and to a method for providing a tomato plant that is resistant to TBRFV at a temperature of at least 28°C.

The invention discloses a composite material, a preparation method of the composite material, a thin film and a photoelectric device, the composite material comprises tobacco mosaic virus and metal nanoparticles, and the metal nanoparticles are combined with coat protein of the tobacco mosaic virus. Part of peptide chains in the coat protein of the tobacco mosaic virus and different types of metal nanoparticles have special binding energy, so that the metal nanoparticles can be orderly assembled on the coat protein of the tobacco mosaic virus, the problem that the metal nanoparticles are easy to agglomerate is effectively improved, and the composite material can be applied to photoelectric devices and has good application prospects. The photoelectric performance and the service life of the photoelectric device can be improved.

The invention relates to the technical field of plant molecular breeding and molecular biology, in particular to a KASP molecular marker related to soybean mosaic virus resistance and application of the KASP molecular marker. The molecular marker is targeted to an anti-soybean mosaic virus specific functional SNP (Single Nucleotide Polymorphism) site of a soybean GmLMM4 gene; the SNP locus is c.743Cgt of a soybean GmLMM4 gene coding region, and the SNP locus is a nucleotide sequence of the soybean GmLMM4 gene; the GmLMM4 gene is mutated by a T mononucleotide, and the 248th amino acid of the GmLMM4 gene coding protein is changed into isoleucine from threonine through the mutation. The molecular marking tool provided by the invention has the advantages that the accuracy of anti-SMV breeding can be remarkably improved, the matched primer group is designed in a targeted antisense strand, and the C / C, C / T and T / T genotypes can be accurately distinguished by FAM / HEX fluorescence signals and correspond to soybean anti-SMV phenotypes; high-throughput detection is completed in the seedling stage, the screening efficiency of high-resistance materials is improved, the identification period is shortened, and the screening efficiency of soybean anti-SMV breeding is improved.

The invention provides a method for creating a transgenic papaya strain with high papaya ringspot virus and malformed mosaic virus resistance, which comprises the following steps: respectively placing CP fusion gene segment expression cassettes of PRSV and PLDMV of papaya and selection marker gene expression cassettes into two independent T-DNA structural domains, and inserting plant expression vectors into the T-DNA structural domains; then introducing the vector into papaya to obtain a papaya strain without a selection marker; or the CP fusion gene segment expression cassettes of the PRSV and the PLDMV and the reverse sequence of the expression cassettes are connected in series and inserted into a plant expression vector, and then the vector is introduced into the papaya, so that the papaya strain with the CP fusion gene segment expression cassettes in reverse series connection is obtained. The papaya without the selection marker does not contain the selection marker, so that the safety can be effectively improved; the papaya in which the CP fusion gene segment expression cassettes are reversely connected in series does not need to be further homozygous to enhance the disease-resistant effect.

The invention belongs to the technical field of crop disease prevention and control, and particularly relates to a planting method for preventing cucumber fruit blotch germs and green mottle viruses, which comprises the following steps: acquiring soil and crop historical pathogen data, environment monitoring data and cucumber seed batch information of a planting area; querying supply chain quarantine records of the seeds, and carrying out standardization treatment on the seeds; after planting, the disease probability of each growth stage is predicted through a risk assessment algorithm, a dynamic monitoring plan is generated in combination with a fuzzy mapping algorithm, plant samples are collected, PCR detection of melon bacterial fruit blotch and RT-PCR detection of cucumber green mottle mosaic virus are carried out, and a quarantine report is issued. Based on the monitoring plan, the quarantine report and the real-time environment data, judging a comprehensive risk level, triggering a corresponding field intervention scheme, and recording an execution result to form an intervention log; after planting is finished, a prevention and control efficiency report is generated. Therefore, the problems of insufficient disease detection, lack of intervention measures and the like in the prior art are solved.

The application belongs to the technical field of virus nucleic acid molecule diagnosis, and particularly relates to a kit for detecting cucumber green mottle mosaic virus based on enzyme-mediated double amplification of nucleic acid, a detection method and application. The detection system of the application comprises a primer pair for amplifying the coat protein gene of the cucumber green mottle mosaic virus, i.e. a DNA upstream primer (SEQ ID NO. 2) and a DNA downstream primer (SEQ ID NO. 3), a probe for detecting the CP gene (an RNA primer (SEQ ID NO. 1)), a nucleic acid amplification enzyme system, a signal amplification enzyme system, a nucleic acid clearing enzyme, an activating enzyme NTP, a buffer, and a freeze-drying protective agent, which are prepared into a pre-dispensed freeze-dried ball. The kit can realize RNA detection at aM concentration, and early screening of the cucumber green mottle mosaic virus can be realized due to high detection sensitivity.

The invention belongs to the technical field of genetic breeding, and relates to a soybean mosaic virus resistance gene Gm13g184900 as well as a molecular marker and application thereof. According to the invention, the Gm13g184900 gene of a soybean variety Suweon 97 is cloned, the nucleotide sequence and the amino acid sequence of the Gm13g184900 gene are respectively shown as SEQ ID NO.1 and SEQ ID NO.2, and experiments prove that the gene inhibits the proliferation of SMV on a leguminous plant cowpea and reduces the protein accumulation amount of SMV on cowpea leaves. Meanwhile, a soybean plant which stably inherits and expresses the gene is obtained, and it is found that SMV infection can be resisted by expressing the gene on soybeans, so that the gene is indeed an effective anti-SMV gene. The invention also provides a specific molecular marker of the gene, and the molecular marker can specifically identify soybean varieties containing 13g184900R / S allele. The Gm13g184900 gene obtained by the invention can be used for improving the soybean mosaic virus resistance of soybeans, and has a great application prospect in the soybean antiviral breeding industry subsequently.

The invention provides a new species of tobamovirus, and its use for identifying plants comprising resistance against the virus.

The invention discloses application of a KASP molecular marker in preparation of a preparation for identifying soybean mosaic virus, and belongs to the technical field of molecular assisted breeding. According to the application of the KASP molecular marker in preparing the preparation for identifying the soybean mosaic virus disease, the KASP molecular marker is located at the 30473748bp position of the No.13 chromosome of a soybean reference genome, and the basic group is G / A. The molecular marker is closely linked with the soybean mosaic virus disease, and can be used for preliminarily screening soybean mosaic virus disease resistant varieties, so that molecular marker-assisted breeding is achieved, and the breeding process is accelerated. In production, the molecular marker can be used for detecting and screening soybean mosaic virus resistant plants in the seedling stage, the screened germplasm carrying excellent disease-resistant allelic genes provides a basis for further phenotype identification and rapid screening and utilization of resistant sources, and the yield per unit area of soybeans is increased.

The invention discloses an application of a wild soybean GsOST1A coding gene in resisting soybean mosaic virus diseases. The GsOST1A encodes a catalytic subunit of an oligosaccharide-based transferase complex, participates in a protein N-glycosylation process in an endoplasmic reticulum, and the nucleotide sequence of the GsOST1A is SEQ ID NO. 1. After the SMV-SC7 is used for treating a plant which overexpresses the gene for 21 days, the SMV content in the overexpressed plant is obviously reduced, which indicates that the resistance of the soybean to the soybean mosaic virus disease can be obviously improved by overexpressing the gene. The wild soybean coding gene GsOST1A disclosed by the invention can be transformed into soybeans through genetic engineering, and the resistance of the soybeans to the soybean mosaic virus diseases is finally regulated and controlled.

The invention discloses a high-throughput screening method for TMV (Tobacco Mosaic Virus) resistant microorganisms. The high-throughput screening method comprises the following specific steps: (1) purifying TMV; (2) activation of a to-be-detected strain: inoculating a single colony into a liquid culture medium, culturing on a constant-temperature shaking table under the conditions of 28 DEG C and 180 r / min for 24-48 hours, and performing co-incubation of the to-be-detected strain and the virus when the OD600 value is within the range of 0.6-1; (3) co-incubation: culturing the bacterial liquid to be detected and viruses on a constant-temperature shaking table for 0-3 days under the conditions of 28 DEG C and 180r / min, and taking 1mL of co-incubation liquid every 24 hours for a subsequent antiviral activity detection test; and (4) antiviral activity detection: detecting the virus concentration after co-incubation by adopting methods such as Elisa, an electron microscope and RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction). According to the invention, the screening efficiency is improved, microorganisms or compound resources with tobacco mosaic virus resisting activity are quickly found, and a new solution is provided for agricultural disease control.