5 results about "Cauliflower mosaic virus" patented technology

The invention provides a primer composition, a micro-fluidic chip, a kit and a method for simultaneously detecting multiple transgenic soybean lines and / or transgenic regulatory elements. The primer composition comprises the following primer groups: a primer group used for detecting a cauliflower mosaic virus 35S promoter, a primer group used for detecting a pea ribulose diphosphate carboxylase gene terminator, a primer group used for detecting a transgenic soybean line GMB151, a primer group used for detecting a transgenic soybean line DP356043, a primer group used for detecting a transgenic soybean line GMB152, a primer group used for detecting a transgenic soybean line DP356043 and a primer group used for detecting a transgenic soybean line DP356043. The primer group is used for detecting a transgenic soybean strain DAS-68416-4, the primer group is used for detecting a transgenic soybean strain DBN-09004-6, and the primer group gene is used for detecting a transgenic soybean strain IND-00410-5. On the basis of an LAMP (Loop-Mediated Isothermal Amplification) means and in combination with a microfluidic technology, five transgenic soybean lines and two transgenic regulatory elements can be synchronously detected at most by single sample adding.

The invention provides an artificial plant bidirectional constitutive strong promoter, and particularly relates to a high-activity short transcription enhancer fragment which is formed by connecting three plant endogenous fragments and a plant virus in series and serves as a transcription activation basic element. A minimum core promoter element Mini 35S from a cauliflower mosaic virus and a minimum core promoter element CsVMV mini-pro functional module from a cassava vein mosaic virus are respectively integrated at two ends, so that the plant constitutive strong promoter with bidirectional starting capability is constructed. According to the invention, a synthetic quadruple STE transcription enhancer, a Mini 35S promoter and a CsVMV mini-pro promoter are functionally coupled to form an expression regulation tool with broad-spectrum adaptability to plant species, and two operably connected target gene expression cassettes can be simultaneously driven by a short regulation sequence to realize synergistic efficient expression in plant cells.

A method of producing a transformed cell or plant body of maize of the present invention includes overexpressing, in a cell or plant body of maize, 1) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 2, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 2, the polypeptide having a function of promoting cell division of maize, or 2) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 15, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 15, the polypeptide having a function of promoting cell division of maize, the overexpressing being controlled by a promoter including a 35S promoter of Cauliflower mosaic virus.

The invention discloses a method for efficiently creating a high-sweetness tomato mutant based on CRISPR / Cas9, and belongs to the technical field of gene engineering. The method comprises the following steps: optimizing a promoter of a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 9) editing carrier, and performing gene editing on Solyc12g099190.1 gene of tomato to obtain the high-sweetness tomato mutant. The target sequence of the gene editing is as shown in SEQ ID NO. 2; the optimization comprises the following steps: replacing an arabidopsis thaliana AtU6 promoter in the CRISPR / Cas9 editing vector with a tomato endogenous SlU6 promoter, and replacing a cauliflower mosaic virus CaMV35S promoter in the CRISPR / Cas9 editing vector with a tomato endogenous SlEF promoter at the same time. The method is high in editing efficiency, and the sweetness of the created tomato editing material is greatly improved under the condition that the phenotype of the tomato editing material is not changed.

The invention discloses a molecular marker combination for identifying a new cauliflower mosaic virus, a primer and application of the molecular marker combination and the primer, and belongs to the technical field of plant virus detection. According to the invention, two types of novel cauliflower mosaic viruses LaCA and LaCB capable of infecting lilies to cause the lilies to be pathogenic are found, and the genomic sequences of the novel cauliflower mosaic viruses LaCA and LaCB are respectively shown as SEQ ID NO.1 and SEQ ID NO.2. The invention designs a molecular marker combination capable of rapidly identifying the two viruses in plants such as lily and the like, and the molecular marker combination comprises molecular markers with sequences as shown in SEQ ID NO.3-8. The two new viruses can be efficiently identified at low cost, technical support is provided for virus prevention and control of plants such as lily, spreading and diffusion of the two viruses are reduced to the maximum extent, and healthy and efficient development of the lily industry is further promoted.