3 results about "Cauliflower mosaic virus" patented technology

The invention provides an artificial plant bidirectional constitutive strong promoter, and particularly relates to a high-activity short transcription enhancer fragment which is formed by connecting three plant endogenous fragments and a plant virus in series and serves as a transcription activation basic element. A minimum core promoter element Mini 35S from a cauliflower mosaic virus and a minimum core promoter element CsVMV mini-pro functional module from a cassava vein mosaic virus are respectively integrated at two ends, so that the plant constitutive strong promoter with bidirectional starting capability is constructed. According to the invention, a synthetic quadruple STE transcription enhancer, a Mini 35S promoter and a CsVMV mini-pro promoter are functionally coupled to form an expression regulation tool with broad-spectrum adaptability to plant species, and two operably connected target gene expression cassettes can be simultaneously driven by a short regulation sequence to realize synergistic efficient expression in plant cells.

A method of producing a transformed cell or plant body of maize of the present invention includes overexpressing, in a cell or plant body of maize, 1) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 2, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 2, the polypeptide having a function of promoting cell division of maize, or 2) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 15, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 15, the polypeptide having a function of promoting cell division of maize, the overexpressing being controlled by a promoter including a 35S promoter of Cauliflower mosaic virus.

The invention discloses a method for efficiently creating a high-sweetness tomato mutant based on CRISPR / Cas9, and belongs to the technical field of gene engineering. The method comprises the following steps: optimizing a promoter of a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 9) editing carrier, and performing gene editing on Solyc12g099190.1 gene of tomato to obtain the high-sweetness tomato mutant. The target sequence of the gene editing is as shown in SEQ ID NO. 2; the optimization comprises the following steps: replacing an arabidopsis thaliana AtU6 promoter in the CRISPR / Cas9 editing vector with a tomato endogenous SlU6 promoter, and replacing a cauliflower mosaic virus CaMV35S promoter in the CRISPR / Cas9 editing vector with a tomato endogenous SlEF promoter at the same time. The method is high in editing efficiency, and the sweetness of the created tomato editing material is greatly improved under the condition that the phenotype of the tomato editing material is not changed.