Primer for testing cauliflower mosaic virus (CaMV) 35S promoter in food and feed
A cauliflower and promoter technology, applied in the field of rapid screening of transgenic products, can solve the problems of expensive reagents and instruments
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Embodiment 1
[0028] Follow the procedure below for testing:
[0029] (1) DNA extraction of the tofu sample to be tested
[0030] A. Weigh 0.1g of tofu, cut it into pieces, and transfer it to a 1.5mL centrifuge tube. Add 600 μL of preheated lysis buffer, mix gently, and keep warm in a water bath at 65°C for 10 minutes;
[0031] B. Add an equal volume of 600 μL of phenol / chloroform into the tube, mix well by inverting up and down, and extract for 2 minutes;
[0032] C. Centrifuge at 12000g for 5min, draw the supernatant into a new centrifuge tube, add the same volume of sedimentation solution as the supernatant, mix well, leave at room temperature for 10min, centrifuge at 12000g for 5min, remove the supernatant, and keep the precipitate;
[0033] D. Add 60 μL of RNase to the precipitate, place it at 37°C for 2 minutes, mix it well with a pipette tip, dissolve the precipitate at 37°C, add 300 μL of buffer solution after 5 minutes, and mix up and down 10 times;
[0034] E. Take out the spin...
Embodiment 2
[0047] Follow the procedure below for testing:
[0048] (1) Extraction of the DNA of the rice sample to be tested
[0049] A. Take an appropriate amount of rice, grind it, weigh 0.1g and transfer it to a 1.5mL centrifuge tube. Add 600 μL of preheated lysis buffer, mix gently, and keep warm in a water bath at 65°C for 10 minutes;
[0050] B. Add an equal volume of 600 μL of phenol / chloroform into the tube, mix well by inverting up and down, and extract for 2 minutes;
[0051] C. Centrifuge at 12000g for 5min, draw the supernatant into a new centrifuge tube, add the same volume of sedimentation solution as the supernatant, mix well, leave at room temperature for 10min, centrifuge at 12000g for 5min, remove the supernatant, and keep the precipitate;
[0052] D. Add 60 μL of RNase to the precipitate, place it at 37°C for 2 minutes, mix it well with a pipette tip, dissolve the precipitate at 37°C, add 300 μL of buffer solution after 5 minutes, and mix up and down 10 times;
[00...
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