Gene chip for detection of hyperpiesis individual medicine correlated gene mutation and uses thereof

A gene chip and high blood pressure technology, applied in the field of gene chips, can solve the problems of low frequency of occurrence of ADRB1, no necessity of detection, and influence on the accuracy of detection results, etc.

Inactive Publication Date: 2009-01-14
湖南宏灏基因生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this invention is that it can only detect mutations in 6 genes, CYP2C9*3, CYP2C19*2, CYP2D6*10, ADRB1(A145G), ADRB1(G1165C), AGTR1(A1166C), among which CYP2C19*2 and hypertension drugs The responsiveness of treatment is not directly related, and the frequency of ADRB1(A145G) is relatively low. Neither of them is necessary t

Method used

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  • Gene chip for detection of hyperpiesis individual medicine correlated gene mutation and uses thereof
  • Gene chip for detection of hyperpiesis individual medicine correlated gene mutation and uses thereof
  • Gene chip for detection of hyperpiesis individual medicine correlated gene mutation and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1: Preparation of gene chip for detection of gene mutations related to individualized medication for hypertension

[0077] 1. The main raw and auxiliary materials and equipment for preparation (as shown in Table 4)

[0078] Table 4

[0079] Reagents and equipment

Origin

Reagent purity / concentration

Blank ultra-flat film

Shanghai Baiao Technology Co., Ltd.

/

Aminosilane reagent

Acros (United States)

99wt.%

Glutaraldehyde

Acros (United States)

25wt.%

95% ethanol

Shanghai Chemical Plant

Analytically pure

Glacial acetic acid

Shanghai Chemical Plant

Analytically pure

NaBH4

Shanghai Chemical Plant

Analytically pure

Na 2 HPO 4

Shanghai Chemical Plant

Analytically pure

KH 2 PO 4

Shanghai Chemical Plant

Analytically pure

Na 2 CO 3

Shanghai Chemical Plant

Analytically pure

NaHCO 3

Shanghai Chemical Plant ...

Embodiment 2

[0102] Example 2: Using the gene chip prepared in Example 1 to treat five hypertension drugs: CYP2C9*3, ADRB1 (1165G>C), AGTR1 (1166A>C), CYP2D6*10, and ACE (I / D) Genetic mutations are detected.

[0103] From August 2005 to June 2006, clinical verification was carried out in the Third Xiangya Hospital of Central South University, the First Affiliated Hospital of Sun Yat-sen University, and Jiangxi Provincial People's Hospital. A total of 1073 samples were tested, and the samples were human peripheral whole blood. For hypertension outpatients and inpatients and other random populations in cities and surrounding areas where each clinical verification unit is located. The clinical verification work is carried out in the clinical verification unit. The clinical verification method is the control method, and the PCR-RFLP genotyping method used routinely in clinical practice and the direct sequencing method as the gold standard are used as the control, and the typing test is performed o...

Embodiment 3

[0150] Example 3: Verify the consistency of the gene chip detection results prepared by the forward probe and the reverse probe

[0151]The present invention can synthesize a set of forward probes (including detection probes and reference probes) and a set of reverse probes (including detection probes and reference probes) for each site. The effect of forward probe and reverse probe in chip detection is the same, provided that the forward probe and the corresponding reverse probe are consistent in length and base arrangement, and each probe at the same detection site The needle direction should be the same, and different detection sites can be randomly selected to synthesize normal phase or reverse probes to form an array. In addition, the PCR primers at each position label the front primer (F primer) when the probe is in the forward direction, and label the rear primer (R primer) when the probe is in the reverse direction. This example aims to verify the consistency of the detect...

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PUM

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Abstract

The invention relates to a gene chip used for detecting gene mutation relating to a high blood pressure personalized medicine. The gene chip for detecting the gene mutation relating to high blood pressure personalized medicine comprises a solid phase support, a gene probe (an oligonueleotide probe) fixed in the solid phase support sequentially and a PCR primer used for amplifying the mutated gene fragment in the sample; the gene probe (the oligonueleotide probe) and the PCR primer are designed aiming at one or two points of the gene mutation in ACE (I/D) and CYP3A5*3 and / or two or more points as follows: CYP2C9*3, CYP2C9*13, AGTR1(A1166C), CYP2D6*10, ADRB1(C1165G), TSC(C1784T), ADRB3(T727C), SCNN1G_rs5729, SCNN1G_rs5723, ENOSA_rs1799983 and GNB3(C825T). The invention provides the gene chip and applications thereof for conveniently, quickly and systematically detecting the gene mutation relating to high blood pressure personalized medicine so as to determine drug reactions.

Description

Technical field [0001] The invention relates to a gene chip used for detection of gene mutations related to individualized medication for hypertension. Background technique [0002] As a major disease that plagues human physical and mental health, hypertension has become a global public health problem: conservatively estimated that the number of hypertensive patients in my country exceeds 160 million, and the incidence is increasing year by year; the treatment of hypertension and its complications has become The economic burden of the world’s third largest disease. Drugs are the main means to treat hypertension and its complications at present and for a long time in the future. Due to individual differences in drug reactions (efficacy and adverse reactions), about 20% to 50% of patients receiving drug treatment have not well controlled blood pressure. [0003] Individual differences in drug response are a very common clinical phenomenon. There are many reasons for this difference...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 周宏灏
Owner 湖南宏灏基因生物科技有限公司
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