Gene chip for detection of hyperpiesis individual medicine correlated gene mutation and uses thereof

Abstract

The invention relates to a gene chip used for detecting gene mutation relating to a high blood pressure personalized medicine. The gene chip for detecting the gene mutation relating to high blood pressure personalized medicine comprises a solid phase support, a gene probe (an oligonueleotide probe) fixed in the solid phase support sequentially and a PCR primer used for amplifying the mutated gene fragment in the sample; the gene probe (the oligonueleotide probe) and the PCR primer are designed aiming at one or two points of the gene mutation in ACE (I / D) and CYP3A5*3 and / or two or more points as follows: CYP2C9*3, CYP2C9*13, AGTR1(A1166C), CYP2D6*10, ADRB1(C1165G), TSC(C1784T), ADRB3(T727C), SCNN1G_rs5729, SCNN1G_rs5723, ENOSA_rs1799983 and GNB3(C825T). The invention provides the gene chip and applications thereof for conveniently, quickly and systematically detecting the gene mutation relating to high blood pressure personalized medicine so as to determine drug reactions.

Description

Technical field [0001] The invention relates to a gene chip used for detection of gene mutations related to individualized medication for hypertension. Background technique [0002] As a major disease that plagues human physical and mental health, hypertension has become a global public health problem: conservatively estimated that the number of hypertensive patients in my country exceeds 160 million, and the incidence is increasing year by year; the treatment of hypertension and its complications has become The economic burden of the world’s third largest disease. Drugs are the main means to treat hypertension and its complications at present and for a long time in the future. Due to individual differences in drug reactions (efficacy and adverse reactions), about 20% to 50% of patients receiving drug treatment have not well controlled blood pressure. [0003] Individual differences in drug response are a very common clinical phenomenon. There are many reasons for this difference...

AI Technical Summary

Problems solved by technology

The disadvantage of this invention is that it can only detect mutations in 6 genes, CYP2C9*3, CYP2C19*2, CYP2D6*10, ADRB1(A145G), ADRB1(G1165C), AGTR1(A1166C), among which CYP2C19*2 and hypertension drugs The responsiveness of treatment is not directly related, and the frequency of ADRB1(A145G) is relatively low. Neither of them is necessary to be detected. The detection results of the remaining 4 gene mutations cannot be fully used to guide the individualized drug treatment of hypertension. In addition, the invention only sets a positive reference probe, and does not set a negative reference probe for each mutation site, which will also affect the accuracy of the detection results

Images