251 results about "Gene Microarray" patented technology

The invention relates to the technical field of crop genetic breeding and plant molecular design breeding, in particular to a pakchoi whole genome liquid phase chip and application. The pakchoi breeding gene chip disclosed by the invention comprises a target capture probe which is designed by taking 1000 core SNP (Single Nucleotide Polymorphism) molecular markers positioned on 40KSNP sites on a pakchoi reference genome version V4.0 as templates. The pakchoi whole genome liquid phase chip provided by the invention comprises a 40K site targeted capture probe mixed solution and a hybridization capture reagent. The pakchoi 40KSNP targeted capture probe can rapidly and effectively track genetic materials of pakchoi, and is suitable for different application scenes such as accurate identification of pakchoi germplasm resources, genetic relationship analysis between materials, variety authenticity identification, QTL gene positioning and mining, molecular marker-assisted breeding, variety improvement and whole genome selective breeding application. Therefore, the method has good application prospect and important social value.

The invention belongs to the technical field of whole-genome gene chips, and particularly relates to a leymus chinensis whole-genome 50K liquid chip and application thereof. According to the leymus chinensis whole genome 50K liquid phase chip, a whole genome re-sequencing technology is utilized, an SNP molecular marker combination of leymus chinensis is determined, and then the leymus chinensis whole genome 50K liquid phase chip is developed. The chip is combined with a high-density SNP probe, realizes high-throughput and high-precision genetic typing of leymus chinensis genomes, and particularly has wide application prospects in the field of molecular breeding.

The invention discloses an SNP (Single Nucleotide Polymorphism) and InDel site combination for identifying variety of Inner Mongolia cattle, a 5K targeted capture gene chip and application of the 5K targeted capture gene chip. The SNP and InDel site combination for identifying the variety of the Inner Mongolia cattle is firstly disclosed and comprises 5000 SNP and InDel sites, and the physical position information of a cattle reference genome with the version number of AR-UCD1.2 is shown in a table 1. The invention further discloses a 5K targeting capture gene chip comprising the probe targeting the SNP and InDel site combination as well as a synthesis method and application of the 5K targeting capture gene chip. The 5K targeted capture gene chip completes genetic typing through a targeted capture probe by means of a high-throughput sequencing technology, has the characteristics of low cost, high efficiency and high accuracy, and can realize the variety identification function of Mongolian cattle, Sanhue cattle and Sunitt cattle by utilizing genotype data; important technical support and specific means are provided for identification and evaluation of Inner Mongolia cattle germplasm resources.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination, a molecular probe combination, a liquid phase chip and a kit for corn genotyping and application of the SNP molecular marker combination, the molecular probe combination, the liquid phase chip and the kit, and belongs to the technical field of gene chips. The SNP molecular marker combination and the liquid chip provided by the invention can be used for efficient and rapid genetic typing of corn germplasm resources, and are low in cost and suitable for corn biological breeding. The SNP marker provided by the invention uniformly covers the whole corn reference genome, provides a high-quality molecular marker for silage corn whole genome selective breeding, and can also be used for map-based cloning of important agronomic trait genes of silage corn.

The invention relates to the technical field of molecular biology, and discloses 74 core molecular marker sets for identifying cold resistance of pennisetum alopecuroides, a primer group, a detection reagent, a kit, a gene chip and application thereof. According to the method, core germplasm resources of pennisetum alopecuroides are systematically collected, and the GWAS population is constructed by utilizing multi-environment (different altitudes) conditions, so that wide genetic and phenotypic variation is fully covered, and a representative material foundation is laid for cold-resistant genetic analysis. In the aspect of phenotype evaluation, a method for determining the number of reviving and root cutting seedlings after natural overwintering by combining single-root double-stem-node oblique cutting planting is innovatively provided, a set of scientific and stable cold resistance and reproducibility evaluation system capable of being operated in a standardized mode is established, and the reliability and efficiency of phenotype identification are remarkably improved. The detection is not influenced by human and climate environments; the method realizes efficient and accurate early cold resistance identification of pennisetum alopecuroides, greatly improves the breeding screening efficiency and scale, and accelerates the breeding process.

The invention provides a self-adaptive brightness classification method and system for sparsely distributed microbeads on a gene chip. The method comprises the following steps: converting gene chip fluorescence image data into a frequency spectrum space by using fast Fourier transform, calculating a rotation angle, and then rotating in a reverse direction to obtain a first image; defining a concerned microbead area on the first image to obtain a second image; performing inverse transformation on the second picture to obtain a third picture, then projecting the third picture and extracting row and column peak values, and finally generating a microbead positioning point array; extracting a foreground value and a background value from the second picture, and outputting a feature gray value at each microbead position; and drawing a histogram according to the feature gray values, determining a threshold value of brightness and darkness division, dividing the beads into bright beads and dark beads, determining the beads with the gray values greater than the threshold value as the bright beads, and determining the beads with the gray values smaller than the threshold value as the dark beads. According to the technical scheme, the method is suitable for microbead positioning and adaptive brightness classification when microbeads are distributed very sparsely, and the positioning success rate is high.

The invention belongs to the technical field of gene detection and gene molecular breeding, and particularly relates to a meat duck whole genome molecular probe combination based on molecular phenotype screening, a 50K gene chip and application thereof. The molecular probe combination and the gene chip of the marker site combination for meat duck whole genome breeding simultaneously cover 7 representative meat duck varieties and 71 economic characters, have richer polymorphism and higher pertinence in meat duck groups, and are lower in cost and higher in speed compared with high-throughput sequencing detection; the breeding chip is designed according to the growth, feed efficiency, slaughtering, breeding, egg quality and various molecular phenotypes of the meat ducks, and compared with a high-throughput sequencing technology, the breeding chip is higher in seed selection accuracy, has higher breeding value, can be widely applied to breeding genotype detection of the meat ducks, and can be used for detecting the breeding genotypes of the meat ducks. And the method has creative significance in the aspect of meat duck genome selective breeding.

The invention relates to the field of pathogen detection, in particular to a primer probe group, a gene chip and a kit for detecting seven waterfowl infectious disease pathogens and application. The primer probe group provided by the invention can be used for simultaneously detecting the goose parvovirus, the duck viral enteritis virus, the muscovy duck parvovirus, the duck hepatitis A virus type 1, the duck hepatitis A virus type 3, the duck tembusu virus and the novel duck reovirus, and is strong in specificity and high in sensitivity. Furthermore, a gene chip prepared by using the primer probe group provided by the invention not only has strong specificity and high sensitivity (1.0 * 100 copies / [mu] L), but also has good repeatability and stability, can be stably stored within at least 180 days, has sensitivity superior to that of a standard fluorescent quantitative PCR method, can rapidly and efficiently detect main epidemic disease pathogens of waterfowl together, and has good application prospects. The method can be used for epidemiological investigation and disease diagnosis of waterfowls.

The invention discloses an oat KASP marker, a fingerprint spectrum and application, and relates to the technical field of molecular breeding. The KASP marker comprises 96 SNP (Single Nucleotide Polymorphism) sites, and the position information and the polymorphism of the KASP marker are shown in a table 1. The invention also provides a gene chip for analyzing the KASP marker and an oat variety characteristic fingerprint spectrum constructed by using the KASP marker, and the KASP marker, the gene chip and the oat variety characteristic fingerprint spectrum provided by the invention can be used for screening, identifying and tracing oat quality. The method can be used for identifying the authenticity of the seeds and the genetic relationship between the parent and offspring of the oat, and has important significance for protecting and improving the germplasm resources of the oat.

The invention discloses a molecular marker combination for analyzing goat varieties and application, relates to the technical field of biology, and provides 13947 molecular markers capable of analyzing goats, and the physical position information of the molecular markers is determined based on genome sequence comparison of a goat reference genome ARS1.2. A molecular probe combination, a gene chip and a kit prepared from 13947 SNP molecular markers can be used for carrying out genetic evaluation on goat individuals, carrying out individual selection on goat varieties which are difficult to determine in the early stage, shortening the generation interval and accelerating the breeding process, so that a large amount of breeding cost is saved.

Provided are a Medicago sativa SNP molecular marker combination and a use thereof. The SNP molecular marker combination consists of 61351 SNP molecular markers. The physical positions of the 61351 SNP molecular markers are determined by sequence alignment on the basis of the Medicago sativa reference genome ZM-4 alfalfa genome. The SNP molecular markers have good site specificity, strong versatility, and high polymorphism and can effectively discriminate Medicago sativa germplasms from different sources. A Medicago sativa genome-wide liquid-phase gene chip is prepared from the SNP molecular markers, and can be used for genetic diversity assessment of Medicago sativa germplasm resources, genetic map construction and functional gene mapping, genome-wide association study, etc., providing an important tool and technical support for Medicago sativa molecular design breeding and variety authenticity identification, and achieving important significance for high-quality development of the alfalfa seed industry in China.

The solid-phase gene chip packaging box comprises a box body, the box body is provided with a containing groove used for containing a first chip or a second chip, the side wall of the containing groove is provided with a first clamping part and a second clamping part, and the first clamping part is located below the second clamping part; wherein the upper surface of the first chip and the upper surface of the second chip are respectively coated with a first protective film and a second protective film; the first clamping part can abut against the upper surface of the first protective film, and the second clamping part can abut against the side portion of the second protective film. According to the solid-phase gene chip packaging box, the first chip or the second chip can be placed in the containing groove, the first chip can be clamped and fixed through the first clamping part, and the second chip can be clamped and fixed through the second clamping part. Therefore, the packaging box can be suitable for packaging two solid-phase gene chips, the universality of the packaging box is improved, and the production cost is reduced.

A gene chip sensor for single nucleotide polymorphism detection and its application belong to the field of biosensor detection technology. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the existing single nucleotide polymorphism detection methods, the present invention provides a gene chip sensor for single nucleotide polymorphism detection, wherein the gene chip sensor comprises WT wild-type and SNP mutant nucleotide sequences, LSL probe, and CRISPR / Cas 12a system. LSL probe is used as a recognition intermediate to activate the CRISPR / Cas12a system, iron-cobalt nanomaterials are used to provide a stable signal source for sample detection, and screen-printed electrodes are combined with electrochemical sensors; the application of CRISPR / Cas12a and nanomaterial technology in molecular breeding is expanded, and technical support is provided for economical and efficient SNP detection.

The application relates to a DNAzyme-based gene chip biosensor and application thereof, and belongs to the technical field of electrochemical sensors. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the single nucleotide polymorphism detection method in the prior art, a DNAzyme-based gene chip biosensor is provided, which comprises a DNAzyme molecular probe SNP recognition system and a solid-liquid phase mixed catalytic network system. The application provides a proteinase-free and space-oriented biosensing strategy, utilizes a molecular switch based on a DNAzyme probe to realize accurate SNP recognition, utilizes a space steric hindrance parallel amplification system to enhance signal amplification and simultaneously inhibit background noise, so that the detection performance is remarkably improved; and the gene chip biosensor can be applied to genotyping and trait selection in crop improvement and other applications.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker associated with a pig backfat thickness character and application. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO: 1; one SNP molecular marker site A / G is arranged at the 254bp position of the sequence of the SNP molecular marker. Genetic typing is carried out by utilizing a gene chip technology, whole genome association analysis is carried out in combination with the character of correcting the 100 kg backfat thickness of a large white pig, one SNP molecular marker remarkably related to the character is screened out, and a new marker resource is provided for molecular breeding of the growth character of the breeding pig.

The invention discloses a gene chip sensor for single nucleotide polymorphism detection and application thereof, and belongs to the technical field of biosensing detection. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost of a single nucleotide polymorphism detection method in the prior art, the invention provides a gene chip sensor for single nucleotide polymorphism detection, and the gene chip sensor comprises WT wild type and SNP mutant nucleotide sequences, an LSL probe and a CRISPR / Cas 12a system. An LSL probe is used as a recognition intermediate to activate a CRISPR / Cas12a system, an iron-cobalt nano material is used to provide a stable signal source for sample detection, and a screen-printed electrode is combined with electrochemical sensing; the application of CRISPR / Cas12a and nanomaterial technology in molecular breeding is expanded, and technical support is provided for economic and effective SNP detection.

Provided is an application of a circular RNA in the preparation of a cerebral stroke diagnosis product, belonging to the field of circular RNA medical treatments. Provided is a kit, comprising a reagent and / or test paper and / or gene chip for detecting a circular RNA-circ-Magi1. The nucleotide sequence of the circular RNA-circ-Magi1 is as shown in SEQ ID NO: 1 or SEQ ID NO: 4. Further provided is the application of a detection system for the diagnosis, assisted diagnosis or prognosis evaluation of cerebral stroke, and a detection reagent of the circular RNA-circ-Magi1 in the preparation of a kit, test paper, chip or high-throughput sequencing platform for the diagnosis, assisted diagnosis or prognosis evaluation of cerebral stroke.

This invention discloses a combination of SNP loci, a gene chip, and their applications for cotton variety identification, relating to the fields of bioinformatics and molecular breeding. From 100 cotton whole-genome resequencing data, this invention screened out 128 SNPs, which can be used as SNP fingerprints for cotton varieties. Using the SNP molecular markers and liquid-phase probes developed in this invention, cotton identification achieved a 100% detection rate, with a minimum allele frequency greater than 0.05, capable of distinguishing any two cotton varieties, enabling efficient and rapid cotton variety identification. Furthermore, this invention utilizes next-generation sequencing technology, resulting in a simple and standardized procedure that reduces human error, leading to extremely low unit costs for batch identification analysis. It is economical and efficient, particularly suitable for large-sample analysis and identification, and has promising application prospects.

The invention provides a cold region corn SNP (Single Nucleotide Polymorphism) liquid phase chip and application thereof, and belongs to the technical field of whole genome gene chips. The invention develops a high-throughput 10K SNP liquid phase chip for corn genotyping on the basis of a targeted capture sequencing technology. The liquid-phase chip is determined through multi-point multi-sample experiments for many years, is high in pertinence, high in reliability and outstanding in practicability, can be used for corn variety identification, hybridization breeding, material heredity basic analysis and fingerprint spectrum construction, and compared with a solid-phase chip, the genetic typing cost is reduced, and a foundation is laid for rapid fusion of corn molecular breeding and conventional breeding. And an important foundation is laid for promoting localization and practicability of a molecular breeding technology.

The present invention relates to a respiratory pathogenic microorganism and drug resistance gene nucleic acid testing kit (gene chip method), comprising a kit body. The kit body comprises a PCR amplification reaction reagent, PCR amplification reaction consumables, gene chip hybridization reaction reagents, gene chip hybridization reaction consumables, and a specific gene probe. The gene chip hybridization reaction reagents include a hybridization solution and a 1000X cleaning solution; the gene chip hybridization reaction consumables include a gene chip and a coverslip; the PCR amplification reaction reagent is a PCR buffer solution; and the PCR amplification reaction consumables are PCR tubes. On the basis of gene chip technology in combination with novel multiplex PCR amplification technology, the present invention provides a novel respiratory pathogenic bacterium and drug resistance gene testing technology which has high testing throughput and sensitivity and good identification accuracy, is simple and easy to operate and low-cost, and is widely used as a conventional testing means; and testing results are used for guiding treatment plans and medications for patients suffering from respiratory diseases and even acute and severe patients.

The invention belongs to the technical field of whole genome gene chips, and particularly relates to a cattle whole genome liquid phase chip and application thereof. Based on an SNP molecular marker combination (position information is shown in a specification table 1) provided by the invention, cattle genotyping can be realized through a targeted capture sequencing technology, and cattle genotypes can be detected in a short time and at high throughput, so that cattle breeding work is promoted. Furthermore, based on the cattle SNP molecular marker combination provided by the invention, a probe is designed, and a whole genome breeding chip is constructed, so that large-scale typing site detection rate of cattle is high, stability is good, and platform wide adaptability is achieved. The method can be applied to germplasm resource identification and mining, genetic diversity analysis, population structure analysis, genetic relationship identification and whole genome selective breeding of cattle varieties, especially Anhui province local cattle varieties, and has important significance in improving the cattle core provenance autonomous breeding efficiency.

The invention discloses a molecular marker combination for analyzing varieties of milk goats and application of the molecular marker combination, relates to the technical field of biology, and provides 2014 molecular markers capable of analyzing the features of the varieties of the milk goats, and the physical position information of the molecular markers is compared and determined based on the genome sequence of a goat reference genome ARS1. A molecular probe combination, a gene chip and a kit prepared from the 2014 SNP molecular markers can be used for carrying out genetic evaluation on goat individuals, individual selection is carried out on milk goat varieties which are difficult to measure in the early stage, the generation interval is shortened, the breeding process is accelerated, and therefore a large amount of breeding cost is saved.

The application relates to the technical field of gene chips, in particular to a SNP molecular marker combination of Pinus massoniana and application thereof. The SNP molecular marker combination is composed of 113,709 SNP molecular markers, wherein the physical position of the SNP molecular markers is determined by sequence alignment based on a reference genome Pinus.tabuliformis V1.0 of Pinus tabulaeformis. The application provides a whole genome chip of Pinus massoniana containing 113,709 SNP sites for the first time, and the chip has the advantages of high efficiency, low cost, good genetic stability and the like, and can be widely applied to different application scenarios such as identification of Pinus massoniana germplasm resources, genetic background analysis of breeding materials, gene positioning, whole genome association analysis, whole genome selection breeding and intelligent design breeding.

The invention belongs to the technical field of pig molecular markers, and particularly discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a pig weaning litter number character and application of the SNP molecular marker. Gene typing is carried out by utilizing a gene chip technology, whole genome association analysis is carried out on the basis of SNP molecular markers, and 11 SNP molecular marker sites related to the weaning litter number character of the binary hybrid sow are screened out through the analysis. The sites of the SNP molecular marker are located on a WDR25 gene segment and a BEGAIN gene segment of a pig chromosome 7, and the SNP molecular marker can be used for marker-assisted breeding of the pig weaning litter number character.

The invention discloses a Chinese rose petal fold whole genome SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof. 968 SNP markers obviously related to petal fold traits are screened out and distributed on 14 chromosomes by analyzing 358 parts of Chinese rose re-sequencing data and utilizing a plurality of software models to carry out whole genome association analysis. A liquid-phase gene chip is designed on the basis of the SNP loci, and plants with ideal petal folding characters can be rapidly screened out through DNA detection in the Chinese rose seedling stage. The liquid phase breeding chip has the advantages of high throughput detection, low cost, shortened breeding period and the like, can simultaneously detect a large number of SNP sites and reduce the cost, can predict future performance of characters in a seed or seedling stage, greatly shortens the breeding period, accelerates cultivation of a new Chinese rose variety with a unique petal rolling character, and has a wide application prospect. The requirements of the market for novel flower types are met, the ornamental value and market competitiveness of the Chinese rose variety are improved, and the development of the flower industry is promoted.

The invention belongs to the technical field of molecular biology, bioinformatics, sorghum genetics and whole-genome gene chips, and particularly relates to a sorghum whole-genome SNP molecular marker combination developed based on a targeted sequencing technology, a 10K liquid chip and application of the sorghum whole-genome SNP molecular marker combination and the 10K liquid chip. The SNP molecular marker combination comprises 10,000 SNP loci, and the specific information of the 10,000 SNP loci is as shown in a table 1; the physical positions of the 10000 SNP loci are determined by performing sequence alignment based on a reference genome of the wine red tassel sorghum variety HYZ-T2T with the version number of v1.0. A liquid chip developed based on the SNP molecular marker combination provided by the invention can be effectively applied to genetic diversity analysis, whole genome association analysis, genetic typing, molecular marker-assisted selective breeding, whole genome selective breeding and other applications of sorghum germplasm resources.

The invention relates to the technical field of gene chips, in particular to a masson pine SNP molecular marker combination and application thereof. The SNP molecular marker combination is composed of 113 SNP molecular markers and 709 SNP molecular markers, wherein the physical positions of the SNP molecular markers are determined by carrying out sequence alignment on the basis of a reference genome Pinus.tabuliformes V1.0 of the Chinese pines. According to the embodiment of the invention, the masson pine whole genome chip containing 113 and 709 SNP sites is provided for the first time, and has the advantages of high efficiency, low cost, good hereditary stability and the like; the method can be widely applied to different application scenes such as pinus massoniana germplasm resource identification, breeding material genetic background analysis, gene localization, whole-genome association analysis, whole-genome selective breeding and intelligent design breeding.

The invention relates to the technical field of biomedicine, and particularly discloses an ARHGAP42 marker for pancreatic cancer diagnosis and prognosis evaluation and application of the ARHGAP42 marker. Experiments prove that the ARHGAP42 is remarkably and highly expressed in pancreatic cancer tissues, and the expression level of the ARHGAP42 is remarkably related to clinical histological grading, lifetime and immunity of a patient. The invention provides application of a reagent for detecting the expression level of ARHGAP42 in preparation of a pancreatic cancer diagnosis or prognosis evaluation tool, including but not limited to qPCR primers, antibodies, ELISA kits or gene chips. In addition, the invention also covers the application of the drug for targeted inhibition of ARHGAP42 in treatment of pancreatic cancer.

The invention belongs to the technical field of pig molecular markers, and particularly discloses an SNP molecular marker related to the character of the number of healthy piglets born by a sow and application of the SNP molecular marker. Genetic typing and whole genome association analysis are carried out on 732 large white sows by using second-generation gene sequencing and gene chip technologies, an SNP site significantly related to the character of the number of healthy piglets born by the large white sows is screened out, the SNP site is located at the upstream of an SLC22A12 gene of a pig chromosome 2 and corresponds to the 7565970th nucleotide of a chromosome 2 of an international pig 11.1 version reference genome, and the SNP site is located at the upstream of the SLC22A12 gene of the chromosome 2 of the pig 11.1 version reference genome and is located at the upstream of the SLC22A12 gene of the chromosome 2 of the pig 11.1 version reference genome. The method can be used for character prediction of healthy litter size of sows or auxiliary breeding.

The application discloses a gene chip and a gene chip detection device. The gene chip comprises a substrate and a positioning device fixed to the upper surface of the substrate; the positioning device is provided with a containing cavity used for containing microbeads; the containing cavity is opened on the surface of the positioning device away from the substrate; and the area of the containing cavity is gradually narrowed in the direction of the height of the positioning device towards the upper surface of the substrate. The gene detection device comprises the gene chip and the microbeads covered with fluorescent probes, and the microbeads are clamped in the containing cavity. The gene chip and the gene chip detection device of the application reduce the reflection of the surface of the containing cavity of the substrate to excitation light, and at the same time, the exposed area of the microbeads is increased. In the process of using the gene chip, the proportion of the fluorescence excited by the fluorescent probes on the microbeads in the light received by the detection device is increased, so that the accuracy of the gene chip detection is improved.