156 results about "Gene Microarray" patented technology

The invention relates to the technical field of molecular biology, and discloses 74 core molecular marker sets for identifying cold resistance of pennisetum alopecuroides, a primer group, a detection reagent, a kit, a gene chip and application thereof. According to the method, core germplasm resources of pennisetum alopecuroides are systematically collected, and the GWAS population is constructed by utilizing multi-environment (different altitudes) conditions, so that wide genetic and phenotypic variation is fully covered, and a representative material foundation is laid for cold-resistant genetic analysis. In the aspect of phenotype evaluation, a method for determining the number of reviving and root cutting seedlings after natural overwintering by combining single-root double-stem-node oblique cutting planting is innovatively provided, a set of scientific and stable cold resistance and reproducibility evaluation system capable of being operated in a standardized mode is established, and the reliability and efficiency of phenotype identification are remarkably improved. The detection is not influenced by human and climate environments; the method realizes efficient and accurate early cold resistance identification of pennisetum alopecuroides, greatly improves the breeding screening efficiency and scale, and accelerates the breeding process.

The invention belongs to the technical field of gene detection and gene molecular breeding, and particularly relates to a meat duck whole genome molecular probe combination based on molecular phenotype screening, a 50K gene chip and application thereof. The molecular probe combination and the gene chip of the marker site combination for meat duck whole genome breeding simultaneously cover 7 representative meat duck varieties and 71 economic characters, have richer polymorphism and higher pertinence in meat duck groups, and are lower in cost and higher in speed compared with high-throughput sequencing detection; the breeding chip is designed according to the growth, feed efficiency, slaughtering, breeding, egg quality and various molecular phenotypes of the meat ducks, and compared with a high-throughput sequencing technology, the breeding chip is higher in seed selection accuracy, has higher breeding value, can be widely applied to breeding genotype detection of the meat ducks, and can be used for detecting the breeding genotypes of the meat ducks. And the method has creative significance in the aspect of meat duck genome selective breeding.

The invention discloses an oat KASP marker, a fingerprint spectrum and application, and relates to the technical field of molecular breeding. The KASP marker comprises 96 SNP (Single Nucleotide Polymorphism) sites, and the position information and the polymorphism of the KASP marker are shown in a table 1. The invention also provides a gene chip for analyzing the KASP marker and an oat variety characteristic fingerprint spectrum constructed by using the KASP marker, and the KASP marker, the gene chip and the oat variety characteristic fingerprint spectrum provided by the invention can be used for screening, identifying and tracing oat quality. The method can be used for identifying the authenticity of the seeds and the genetic relationship between the parent and offspring of the oat, and has important significance for protecting and improving the germplasm resources of the oat.

Provided are a Medicago sativa SNP molecular marker combination and a use thereof. The SNP molecular marker combination consists of 61351 SNP molecular markers. The physical positions of the 61351 SNP molecular markers are determined by sequence alignment on the basis of the Medicago sativa reference genome ZM-4 alfalfa genome. The SNP molecular markers have good site specificity, strong versatility, and high polymorphism and can effectively discriminate Medicago sativa germplasms from different sources. A Medicago sativa genome-wide liquid-phase gene chip is prepared from the SNP molecular markers, and can be used for genetic diversity assessment of Medicago sativa germplasm resources, genetic map construction and functional gene mapping, genome-wide association study, etc., providing an important tool and technical support for Medicago sativa molecular design breeding and variety authenticity identification, and achieving important significance for high-quality development of the alfalfa seed industry in China.

The solid-phase gene chip packaging box comprises a box body, the box body is provided with a containing groove used for containing a first chip or a second chip, the side wall of the containing groove is provided with a first clamping part and a second clamping part, and the first clamping part is located below the second clamping part; wherein the upper surface of the first chip and the upper surface of the second chip are respectively coated with a first protective film and a second protective film; the first clamping part can abut against the upper surface of the first protective film, and the second clamping part can abut against the side portion of the second protective film. According to the solid-phase gene chip packaging box, the first chip or the second chip can be placed in the containing groove, the first chip can be clamped and fixed through the first clamping part, and the second chip can be clamped and fixed through the second clamping part. Therefore, the packaging box can be suitable for packaging two solid-phase gene chips, the universality of the packaging box is improved, and the production cost is reduced.

The application relates to a DNAzyme-based gene chip biosensor and application thereof, and belongs to the technical field of electrochemical sensors. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the single nucleotide polymorphism detection method in the prior art, a DNAzyme-based gene chip biosensor is provided, which comprises a DNAzyme molecular probe SNP recognition system and a solid-liquid phase mixed catalytic network system. The application provides a proteinase-free and space-oriented biosensing strategy, utilizes a molecular switch based on a DNAzyme probe to realize accurate SNP recognition, utilizes a space steric hindrance parallel amplification system to enhance signal amplification and simultaneously inhibit background noise, so that the detection performance is remarkably improved; and the gene chip biosensor can be applied to genotyping and trait selection in crop improvement and other applications.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker associated with a pig backfat thickness character and application. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO: 1; one SNP molecular marker site A / G is arranged at the 254bp position of the sequence of the SNP molecular marker. Genetic typing is carried out by utilizing a gene chip technology, whole genome association analysis is carried out in combination with the character of correcting the 100 kg backfat thickness of a large white pig, one SNP molecular marker remarkably related to the character is screened out, and a new marker resource is provided for molecular breeding of the growth character of the breeding pig.

Provided is an application of a circular RNA in the preparation of a cerebral stroke diagnosis product, belonging to the field of circular RNA medical treatments. Provided is a kit, comprising a reagent and / or test paper and / or gene chip for detecting a circular RNA-circ-Magi1. The nucleotide sequence of the circular RNA-circ-Magi1 is as shown in SEQ ID NO: 1 or SEQ ID NO: 4. Further provided is the application of a detection system for the diagnosis, assisted diagnosis or prognosis evaluation of cerebral stroke, and a detection reagent of the circular RNA-circ-Magi1 in the preparation of a kit, test paper, chip or high-throughput sequencing platform for the diagnosis, assisted diagnosis or prognosis evaluation of cerebral stroke.

This invention discloses a combination of SNP loci, a gene chip, and their applications for cotton variety identification, relating to the fields of bioinformatics and molecular breeding. From 100 cotton whole-genome resequencing data, this invention screened out 128 SNPs, which can be used as SNP fingerprints for cotton varieties. Using the SNP molecular markers and liquid-phase probes developed in this invention, cotton identification achieved a 100% detection rate, with a minimum allele frequency greater than 0.05, capable of distinguishing any two cotton varieties, enabling efficient and rapid cotton variety identification. Furthermore, this invention utilizes next-generation sequencing technology, resulting in a simple and standardized procedure that reduces human error, leading to extremely low unit costs for batch identification analysis. It is economical and efficient, particularly suitable for large-sample analysis and identification, and has promising application prospects.

The application relates to the technical field of gene chips, in particular to a SNP molecular marker combination of Pinus massoniana and application thereof. The SNP molecular marker combination is composed of 113,709 SNP molecular markers, wherein the physical position of the SNP molecular markers is determined by sequence alignment based on a reference genome Pinus.tabuliformis V1.0 of Pinus tabulaeformis. The application provides a whole genome chip of Pinus massoniana containing 113,709 SNP sites for the first time, and the chip has the advantages of high efficiency, low cost, good genetic stability and the like, and can be widely applied to different application scenarios such as identification of Pinus massoniana germplasm resources, genetic background analysis of breeding materials, gene positioning, whole genome association analysis, whole genome selection breeding and intelligent design breeding.

The invention discloses a Chinese rose petal fold whole genome SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof. 968 SNP markers obviously related to petal fold traits are screened out and distributed on 14 chromosomes by analyzing 358 parts of Chinese rose re-sequencing data and utilizing a plurality of software models to carry out whole genome association analysis. A liquid-phase gene chip is designed on the basis of the SNP loci, and plants with ideal petal folding characters can be rapidly screened out through DNA detection in the Chinese rose seedling stage. The liquid phase breeding chip has the advantages of high throughput detection, low cost, shortened breeding period and the like, can simultaneously detect a large number of SNP sites and reduce the cost, can predict future performance of characters in a seed or seedling stage, greatly shortens the breeding period, accelerates cultivation of a new Chinese rose variety with a unique petal rolling character, and has a wide application prospect. The requirements of the market for novel flower types are met, the ornamental value and market competitiveness of the Chinese rose variety are improved, and the development of the flower industry is promoted.

The invention belongs to the technical field of molecular biology, bioinformatics, sorghum genetics and whole-genome gene chips, and particularly relates to a sorghum whole-genome SNP molecular marker combination developed based on a targeted sequencing technology, a 10K liquid chip and application of the sorghum whole-genome SNP molecular marker combination and the 10K liquid chip. The SNP molecular marker combination comprises 10,000 SNP loci, and the specific information of the 10,000 SNP loci is as shown in a table 1; the physical positions of the 10000 SNP loci are determined by performing sequence alignment based on a reference genome of the wine red tassel sorghum variety HYZ-T2T with the version number of v1.0. A liquid chip developed based on the SNP molecular marker combination provided by the invention can be effectively applied to genetic diversity analysis, whole genome association analysis, genetic typing, molecular marker-assisted selective breeding, whole genome selective breeding and other applications of sorghum germplasm resources.

The invention relates to the technical field of biomedicine, and particularly discloses an ARHGAP42 marker for pancreatic cancer diagnosis and prognosis evaluation and application of the ARHGAP42 marker. Experiments prove that the ARHGAP42 is remarkably and highly expressed in pancreatic cancer tissues, and the expression level of the ARHGAP42 is remarkably related to clinical histological grading, lifetime and immunity of a patient. The invention provides application of a reagent for detecting the expression level of ARHGAP42 in preparation of a pancreatic cancer diagnosis or prognosis evaluation tool, including but not limited to qPCR primers, antibodies, ELISA kits or gene chips. In addition, the invention also covers the application of the drug for targeted inhibition of ARHGAP42 in treatment of pancreatic cancer.

The application discloses a gene chip and a gene chip detection device. The gene chip comprises a substrate and a positioning device fixed to the upper surface of the substrate; the positioning device is provided with a containing cavity used for containing microbeads; the containing cavity is opened on the surface of the positioning device away from the substrate; and the area of the containing cavity is gradually narrowed in the direction of the height of the positioning device towards the upper surface of the substrate. The gene detection device comprises the gene chip and the microbeads covered with fluorescent probes, and the microbeads are clamped in the containing cavity. The gene chip and the gene chip detection device of the application reduce the reflection of the surface of the containing cavity of the substrate to excitation light, and at the same time, the exposed area of the microbeads is increased. In the process of using the gene chip, the proportion of the fluorescence excited by the fluorescent probes on the microbeads in the light received by the detection device is increased, so that the accuracy of the gene chip detection is improved.

The present invention discloses a molecular marker combination and application for analyzing sheep breeds, relating to the field of biotechnology. The present invention provides 10,082 molecular markers capable of analyzing sheep, wherein the physical location information of the molecular markers is determined based on genome sequence alignment of the sheep reference genome Oar_rambouillet_v1.0. A molecular probe combination, a gene chip, and a kit prepared using the 10,082 SNP molecular markers can be used to perform genetic assessment on individual sheep, perform individual selection on sheep breeds that were difficult to measure in the early stages, shorten the generation interval, accelerate the breeding process, and thus save a large amount of breeding costs.

The application discloses a kind of gene microarray data analysis processing methods, it is related to biomedical information processing technical field, comprising: using microarray technology, obtain gene expression data;Multiple low-dimensional feature selection tasks are constructed;Optimal feature subset is obtained by multi-task pseudo-affine transformation algorithm;According to optimal feature subset, neural network model is trained;Analysis processing is carried out to the gene to be predicted and whether it is predicted to be ill.The gene microarray data analysis processing method of the application is analyzed to gene microarray data characteristics, and gene microarray data is helpful to dig disease characteristic gene information, and has a key role to early detection, clinical treatment and disease prevention of disease.

The invention provides an SNP (Single Nucleotide Polymorphism) site combination, a molecular probe combination, a zucchini whole genome gene chip and application thereof, the SNP site combination comprises at least one SNP site in a table 1, and physical position information of the SNP site in the table 1 is determined based on a zucchini reference genome 22S20 or genome sequence comparison of an updated version of the zucchini reference genome 22S20. Specifically, the invention provides a zucchini whole genome 10K liquid-phase gene chip and application thereof. By adopting the zucchini whole genome 10K liquid-phase gene chip disclosed by the invention, high-depth re-sequencing can be carried out on target sites of zucchini, and accurate detection of genotypes of SNP (Single Nucleotide Polymorphism) sites is realized. Meanwhile, the zucchini whole genome 10K liquid-phase gene chip has the advantages of low cost and high efficiency, is beneficial to accelerating the breeding process of zucchini, and provides powerful technical support for development and utilization of zucchini germplasm resources.

The application belongs to the technical field of pig molecular marker, and particularly discloses a SNP molecular marker related to the litter size of sows and application thereof. SLC22A12 A SNP site significantly related to the litter size of Landrace sows is screened out by using second-generation gene sequencing and gene chip technology for genotyping and whole genome association analysis of 732 Landrace sows, the SNP site is located on the upstream of a gene on chromosome 2 of pigs, corresponding to the 7565970th nucleotide on chromosome 2 of the international pig 11.1 version reference genome, and can be used for predicting or assisting breeding of the litter size of sows.

The application relates to the technical field of molecular biology, and discloses a cold-tolerance identification method of Pennisetum based on a 65-core molecular marker set, a primer group and a gene chip and application thereof. The application collects core germplasm resources of the Pennisetum through a system, and constructs a GWAS population by using multi-environment (different altitudes) conditions, so that a wide genetic background and phenotype variation are fully covered, and a material foundation for genetic analysis of cold tolerance is laid. In the aspect of cold-tolerance phenotype identification, the application innovatively takes the chlorophyll content of leaves and the percentage of green leaves after natural low-temperature stress in autumn and winter fields as the cold-tolerance evaluation indexes, judges individuals with more than 40 excellent allelic variations as high-cold-tolerance varieties, and establishes a rapid and non-destructive phenotype screening method which can be implemented at the seedling stage.

1. The name of the design product: gene chip detector. 2. The use of the design product: for detecting gene chip, applied to the fields of genotyping, molecular breeding, etc. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1. 5. The bottom of the design product is the part that is not easy to see or cannot be seen during use, so the top view is omitted.

The invention provides a cold-region low-temperature-resistant germination rice gene chip and application thereof, the cold-region low-temperature-resistant germination rice gene chip comprises 1016 SNP sites distributed in 12 chromosomes, is derived from large-scale GWAS analysis of 216 cold-region germplasms, and has the characteristics of high polymorphism, low deletion rate and low heterozygosis rate. The preparation method comprises the following steps: extracting DNA (Deoxyribonucleic Acid), sequencing, comparing and screening associated sites, and designing a specific probe based on the associated sites to prepare a liquid phase chip. The chip shows high precision (genotype consistency is greater than or equal to 98%) and high efficiency in application, and is mainly used for rapid screening, whole genome selective breeding and character correlation analysis of germplasm resources of the low-temperature-resistant rice in the cold region. By means of the chip, the breeding cycle can be shortened from traditional 6-8 generations to 3-4 generations, the screening efficiency is improved by 4-5 times compared with traditional phenotype identification, and a powerful molecular tool and a complete solution are provided for accurate breeding of low-temperature-resistant varieties of the cold-region rice.

The application discloses a pig 80K functional site gene chip and application thereof, and first designs a gene chip by using pig genome promoter, enhancer, coding region key mutation and the like information, classifies functional sites according to importance based on large-scale multi-omics high-throughput sequencing data, develops a functional site gene chip mainly taking functional sites as the main part, breaks the linkage dependence theory, and solves the problem that the existing commercial chip has poor commonality among different populations due to different linkage degrees among different populations. The chip also has the characteristics of high capture property, low cost and flexibility, and is suitable for commercial application such as pig breed genetic breeding research and selection and matching.

The invention discloses a Chinese rose leaf gloss whole genome SNP molecular marker combination and application thereof, the SNP molecular marker combination comprises 4746 SNP sites distributed on 14 chromosomes of a Chinese rose, and 4746 SNP markers significantly related to leaf gloss are screened by analyzing 358 parts of Chinese rose re-sequencing data and distributed on the 14 chromosomes. A liquid-phase gene chip is designed on the basis of the SNP loci, gene detection can be carried out in the seedling stage of the Chinese rose, and the leaf gloss character can be predicted in advance. Compared with a traditional breeding method, the method has the advantages of high throughput detection, low cost, shortened breeding period and the like, the breeding efficiency can be remarkably improved, the cultivation of a new variety of Chinese rose with good glossiness is accelerated, the requirement of the market for high-quality Chinese rose is met, and the development of the flower industry is promoted.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker associated with correction of a 100kg backfat thickness character of a pig and application of the SNP molecular marker. The SNP molecular marker is located on a pig chromosome 2 and contains six SNP molecular marker sites. Genetic typing is carried out by utilizing a gene chip technology, whole genome association analysis is carried out in combination with the 100kg backfat thickness correction character of the landrace, six SNP molecular markers obviously related to the character are screened out, and a new marker resource is provided for molecular breeding of the growth character of the boar.

The invention provides an InDel marker for detecting tomato gray leaf spot disease resistance. The InDel marker is closely linked with a gene of a sixth chromosome SL4.0 ch06: 33855052-33933438 of a tomato. The InDel marker developed by the invention can realize accurate, rapid and high-throughput detection of the tomato gray leaf spot disease resistant sm6.1 gene, and can be applied to commercial breeding of tomatoes and genetic improvement application research of gray leaf spot disease resistance; important clues can be provided for research on group evolution, gene chips and the like.

This invention belongs to the field of whole-genome gene chip technology, specifically relating to a liquid-phase whole-genome chip for cattle and its applications. Based on the SNP molecular marker combination provided by this invention (location information is shown in Table 1 of the specification), genotyping of cattle can be achieved through targeted capture sequencing technology, enabling high-throughput detection of cattle genotypes in a short time, thereby promoting cattle breeding. Furthermore, probes designed based on the cattle SNP molecular marker combination provided by this invention can be used to construct a whole-genome breeding chip, which can achieve high detection rates and stability for large-scale genotyping of cattle, and has broad platform applicability. It can be applied to the identification and mining of germplasm resources, genetic diversity analysis, population structure analysis, kinship identification, and whole-genome selection breeding of cattle breeds, especially local cattle breeds in Anhui Province, which is of great significance for improving the efficiency of independent breeding of core cattle breeds.

The invention provides a larch pine caterpillar resistance gene mining method based on GWAS and multi-omics data, and belongs to the technical field of gene mining. The method comprises the following steps: S1, carrying out insect-resistant phenotype identification on larch individuals, and carrying out variation identification on the larch individuals based on a liquid-phase gene chip sequencing technology; s2, using a mixed linear model to perform correlation analysis by taking a plot position and phenotype observation time as an interaction environment, taking population density at different observation time as a covariable and taking a larch anti-deciduous pine caterpillar index as a phenotype, and identifying a candidate QTL region; and S3, carrying out transcriptome sequencing on resistance extreme individuals, carrying out variation recognition, comparing read segments to the candidate QTL region, and manually analyzing and annotating a gene structure in the QTL region by using IGV. According to the method disclosed by the invention, low-cost variation recognition, environment noise weakening whole genome association analysis and gene structure accurate annotation on complex genome species are realized.

The invention discloses a gemcitabine drug sensitive marker, a detection kit and application of the gemcitabine drug sensitive marker, the gemcitabine drug sensitive marker is a gemcitabine drug-resistant marker or / and a gemcitabine sensitive marker, and the gemcitabine drug-resistant marker is one or more of ATOH8, DAPK2, BCL2L2, PNPLA2, SHROOM1, FGGY, CPQ, CPO, SLC10A5, ADAMTS15, COL25A1, ASMTL and ENSG00000286264; the sensitive marker is one or more of FAM163A, KREMEN2, VPS37D, GGCT (Growth Growth Computed Tomography), ADSL (Asymmetrical Digital Subscriber Loop), FOXL2 (Focal OXL2), MAST1 (MAST1), DNAAF3 (Deoxyribose Nucleic Acid Amplified The gemcitabine drug sensitive marker is used for predicting the drug effect of gemcitabine. According to the present invention, the expression of the related molecules is detected through the sequencing of the tissue transcriptome of the urothelial carcinoma patient, or through the PCR, the gene chip, the tissue chip, the NanoString technology, the immunohistochemistry and the ELISA method, and the guidance is provided for the drug use of gemcitabine and the precise treatment of bladder cancer.

The invention discloses a pig whole genome breeding functional site gene chip and application thereof. The chip contains a probe for detecting 42476 SNP (Single Nucleotide Polymorphism) sites, and the 42476 SNP sites are functional sites related to growth, reproduction and slaughter traits of pigs; the 42KSNP sequencing chip designed by the invention contains a large number of functional sites related to the growth, reproduction and slaughter traits of pigs, so that the breeding accuracy of the growth, reproduction and slaughter traits can be effectively improved in the breeding process, the genetic progress is remarkably improved, and the breeding cycle is shortened.

This invention discloses a haplotype molecular marker associated with thousand-grain weight in rice and its application, belonging to the field of plant molecular breeding technology. The haplotype molecular marker provided by this invention consists of multiple tightly linked SNPs, and its association with the "significantly increased thousand-grain weight" phenotype is extremely significant. P The value is <0.01), and it has good stability. By detecting this haplotype molecular marker, the thousand-grain weight performance of rice can be predicted quickly and accurately at the seedling stage without waiting for the grains to mature, which greatly improves the breeding efficiency. In addition, this haplotype molecular marker can be directly used for molecular marker-assisted breeding of high-yield or large-grain rice varieties, or it can be integrated into rice functional gene chips as a core marker, or used to develop low-cost PCR detection kits, which is highly practical. It provides a direct molecular tool for the genetic improvement of rice thousand-grain weight, which helps to achieve the targeted breeding of high-yield, large-grain rice varieties.