82 results about "Gene Microarray" patented technology

The invention discloses an oat KASP marker, a fingerprint spectrum and application, and relates to the technical field of molecular breeding. The KASP marker comprises 96 SNP (Single Nucleotide Polymorphism) sites, and the position information and the polymorphism of the KASP marker are shown in a table 1. The invention also provides a gene chip for analyzing the KASP marker and an oat variety characteristic fingerprint spectrum constructed by using the KASP marker, and the KASP marker, the gene chip and the oat variety characteristic fingerprint spectrum provided by the invention can be used for screening, identifying and tracing oat quality. The method can be used for identifying the authenticity of the seeds and the genetic relationship between the parent and offspring of the oat, and has important significance for protecting and improving the germplasm resources of the oat.

The solid-phase gene chip packaging box comprises a box body, the box body is provided with a containing groove used for containing a first chip or a second chip, the side wall of the containing groove is provided with a first clamping part and a second clamping part, and the first clamping part is located below the second clamping part; wherein the upper surface of the first chip and the upper surface of the second chip are respectively coated with a first protective film and a second protective film; the first clamping part can abut against the upper surface of the first protective film, and the second clamping part can abut against the side portion of the second protective film. According to the solid-phase gene chip packaging box, the first chip or the second chip can be placed in the containing groove, the first chip can be clamped and fixed through the first clamping part, and the second chip can be clamped and fixed through the second clamping part. Therefore, the packaging box can be suitable for packaging two solid-phase gene chips, the universality of the packaging box is improved, and the production cost is reduced.

The application relates to a DNAzyme-based gene chip biosensor and application thereof, and belongs to the technical field of electrochemical sensors. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the single nucleotide polymorphism detection method in the prior art, a DNAzyme-based gene chip biosensor is provided, which comprises a DNAzyme molecular probe SNP recognition system and a solid-liquid phase mixed catalytic network system. The application provides a proteinase-free and space-oriented biosensing strategy, utilizes a molecular switch based on a DNAzyme probe to realize accurate SNP recognition, utilizes a space steric hindrance parallel amplification system to enhance signal amplification and simultaneously inhibit background noise, so that the detection performance is remarkably improved; and the gene chip biosensor can be applied to genotyping and trait selection in crop improvement and other applications.

Provided is an application of a circular RNA in the preparation of a cerebral stroke diagnosis product, belonging to the field of circular RNA medical treatments. Provided is a kit, comprising a reagent and / or test paper and / or gene chip for detecting a circular RNA-circ-Magi1. The nucleotide sequence of the circular RNA-circ-Magi1 is as shown in SEQ ID NO: 1 or SEQ ID NO: 4. Further provided is the application of a detection system for the diagnosis, assisted diagnosis or prognosis evaluation of cerebral stroke, and a detection reagent of the circular RNA-circ-Magi1 in the preparation of a kit, test paper, chip or high-throughput sequencing platform for the diagnosis, assisted diagnosis or prognosis evaluation of cerebral stroke.

The application relates to the technical field of molecular biology, and discloses a cold-tolerance identification method of Pennisetum based on a 65-core molecular marker set, a primer group and a gene chip and application thereof. The application collects core germplasm resources of the Pennisetum through a system, and constructs a GWAS population by using multi-environment (different altitudes) conditions, so that a wide genetic background and phenotype variation are fully covered, and a material foundation for genetic analysis of cold tolerance is laid. In the aspect of cold-tolerance phenotype identification, the application innovatively takes the chlorophyll content of leaves and the percentage of green leaves after natural low-temperature stress in autumn and winter fields as the cold-tolerance evaluation indexes, judges individuals with more than 40 excellent allelic variations as high-cold-tolerance varieties, and establishes a rapid and non-destructive phenotype screening method which can be implemented at the seedling stage.

1. The name of the design product: gene chip detector. 2. The use of the design product: for detecting gene chip, applied to the fields of genotyping, molecular breeding, etc. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1. 5. The bottom of the design product is the part that is not easy to see or cannot be seen during use, so the top view is omitted.

The invention provides a cold-region low-temperature-resistant germination rice gene chip and application thereof, the cold-region low-temperature-resistant germination rice gene chip comprises 1016 SNP sites distributed in 12 chromosomes, is derived from large-scale GWAS analysis of 216 cold-region germplasms, and has the characteristics of high polymorphism, low deletion rate and low heterozygosis rate. The preparation method comprises the following steps: extracting DNA (Deoxyribonucleic Acid), sequencing, comparing and screening associated sites, and designing a specific probe based on the associated sites to prepare a liquid phase chip. The chip shows high precision (genotype consistency is greater than or equal to 98%) and high efficiency in application, and is mainly used for rapid screening, whole genome selective breeding and character correlation analysis of germplasm resources of the low-temperature-resistant rice in the cold region. By means of the chip, the breeding cycle can be shortened from traditional 6-8 generations to 3-4 generations, the screening efficiency is improved by 4-5 times compared with traditional phenotype identification, and a powerful molecular tool and a complete solution are provided for accurate breeding of low-temperature-resistant varieties of the cold-region rice.

The invention discloses a pig whole genome breeding functional site gene chip and application thereof. The chip contains a probe for detecting 42476 SNP (Single Nucleotide Polymorphism) sites, and the 42476 SNP sites are functional sites related to growth, reproduction and slaughter traits of pigs; the 42KSNP sequencing chip designed by the invention contains a large number of functional sites related to the growth, reproduction and slaughter traits of pigs, so that the breeding accuracy of the growth, reproduction and slaughter traits can be effectively improved in the breeding process, the genetic progress is remarkably improved, and the breeding cycle is shortened.

This invention discloses a haplotype molecular marker associated with thousand-grain weight in rice and its application, belonging to the field of plant molecular breeding technology. The haplotype molecular marker provided by this invention consists of multiple tightly linked SNPs, and its association with the "significantly increased thousand-grain weight" phenotype is extremely significant. P The value is <0.01), and it has good stability. By detecting this haplotype molecular marker, the thousand-grain weight performance of rice can be predicted quickly and accurately at the seedling stage without waiting for the grains to mature, which greatly improves the breeding efficiency. In addition, this haplotype molecular marker can be directly used for molecular marker-assisted breeding of high-yield or large-grain rice varieties, or it can be integrated into rice functional gene chips as a core marker, or used to develop low-cost PCR detection kits, which is highly practical. It provides a direct molecular tool for the genetic improvement of rice thousand-grain weight, which helps to achieve the targeted breeding of high-yield, large-grain rice varieties.

This invention provides a rapid hybridization reaction device for gene chips based on microfluidics, comprising a microfluidic layer based on microfluidic technology, a DNA probe microarray layer corresponding to the microfluidic channels in a dotted array, a temperature control module for providing the hybridization temperature between the DNA probes and DNA in the sample, and a fixing device that fixes and seals the microfluidic layer, DNA probe microarray layer, and temperature control module from top to bottom. Each microfluidic channel is in a continuous U-shaped configuration (positive-negative-positive-U), and is equipped with a liquid flow drive module for driving the DNA solution to flow back and forth within the microfluidic channel. After sealing, all probe array points are located within an independent microfluidic channel, and a sample dispensing orifice is provided at the upper end of the fixing device for adding samples to each independent microfluidic channel. Through the synergistic effect of temperature and flow rate, this device can reduce sample consumption, increase hybridization rate, reduce hybridization time, and avoid cross-contamination.

Disclosed is a gene panel which can evaluate lung adenocarcinoma molecular subtype and survival risk, and an application of a reagent, which detects the gene expression level of the gene panel, in preparing a product. The product is used for determining lung adenocarcinoma molecular subtype and evaluating lung adenocarcinoma patient survival risk. The product comprises a Next-Generation Sequencing (NGS) detection reagent kit, a fluorescence quantitative PCR detection reagent kit, a gene chip and a protein microarray. Also disclosed is a method which uses the detection reagent kits to evaluate lung adenocarcinoma molecular subtype and survival risk.

The application discloses an intestinal microbial marker combination for cirrhosis diagnosis and application thereof, which is composed of 8 specific intestinal bacterial species. The application accurately identifies the core combination from 181 differential characteristics by performing metagenomic sequencing on fecal samples of cirrhosis patients and healthy controls, and combining random forest, LASSO regression and recursive feature elimination algorithms. The random forest diagnosis model based on the 8 markers realizes an excellent performance of area under curve (AUC) 0.841, sensitivity 84.3% and specificity 83.7% on a completely independent test set, and the performance does not decrease compared with the full feature model in the case of reducing the number of characteristics by 86.9%. The marker combination provided by the application is highly simplified and has strong discrimination, and provides a clear and efficient microbiological basis and technical scheme for developing a cirrhosis non-invasive diagnosis kit, a gene chip and other products based on intestinal flora.

The invention relates to the technical field of gene chips, in particular to a sorghum whole genome liquid phase chip as well as a construction method and application thereof. A detection target of the sorghum whole genome liquid phase chip disclosed by the invention is composed of 5446 SNP molecular markers and 3 InDel molecular markers; the positions of the 5446 SNP molecular markers and the 3 InDel molecular markers, which are positioned on a sorghum reference genome Sorghumbicolv3.1. 1, are as shown in a table 1; the sorghum whole genome liquid chip can be used for sorghum variety identification, genetic relationship identification, genetic diversity analysis, molecular genetic map construction, gene positioning and cloning, molecular marker-assisted selection and whole genome selective breeding, and provides an important molecular basis for important agronomic trait analysis and biological breeding of sorghum.

The invention discloses a DNA (Deoxyribose Nucleic Acid) fingerprint spectrum for identifying a feeding oat variety and application thereof, relates to the technical field of biology, and provides 21 molecular markers capable of analyzing and identifying the feeding oat variety. A molecular probe combination, a gene chip and a kit prepared from the 21 SNP molecular markers can play an important role in the aspects of the blood relationship and genetic basis of oat crops, variety certification and determination, counterfeit and shoddy seed identification, management and detection of genetic breeding and crop maps and the like.

The application discloses a Holstein cow breeding probe combination, a gene chip, a kit and application, the probe combination can detect 140290 SNP sites based on the reference genome ARS-UCD1.2. The site detected by the Holstein cow breeding probe combination is obtained from 77887 sites after further screening based on 4600 Holstein cow genome sequencing data, in combination with 62387 SNP sites related to yield, reproduction and body shape and 16 defect-related SNP sites, and can effectively reflect the structural heterogeneity of the genome itself. The site detected by the Holstein cow breeding probe combination has high polymorphism information content, and fully covers high polymorphism sites. Meanwhile, the interpolation accuracy is high, the genotyping consistency is equivalent to that of a high-density chip, the cost is significantly lower, and the economy is better, and the application provides an independent technical tool for efficient and accurate genome breeding of Holstein cows.

The invention belongs to the technical field of fish breeding and biochips, and particularly relates to a micropterus salmoides SNP (Single Nucleotide Polymorphism) chip and application thereof. A specific capture probe is designed based on the provided largemouth bass SNP molecular marker combination, a gene chip for whole genome genotyping of largemouth bass is constructed, the gene chip is used for accurate and comprehensive detection of whole genome genotyping of largemouth bass, and the gene chip has the advantages of high detection flux, low cost, flexible customization and the like; the method is used for germplasm resource identification, genetic diversity analysis, whole genome association analysis, important functional gene mining and selective breeding of the micropterus salmoides, and a novel and efficient tool is provided for accelerating fine variety breeding of the micropterus salmoides.

The application relates to the establishment of an early gastric cancer recurrence prediction model. By using two batches of gene chip transcriptome data GSE130823 and GSE55696, 25 potential genes related to early gastric cancer recurrence are screened out, and an early gastric cancer recurrence prediction model based on eight genes AREG, LOC100507520, MMD, CH3L1, FOS, CCL20, CXCR2 and BATF3 is established. The model has excellent sensitivity, that is, all the patients predicted to not relapse do not relapse, and the frequency of reexamination and follow-up of the patients can be adjusted according to the model.

The invention discloses a method for detecting a multi-target gene editing plant based on a liquid-phase gene chip. The invention provides a liquid phase chip. The liquid phase chip comprises a capture probe, the capture probe is designed and synthesized according to the following method: a target region is selected within the upstream and downstream range of not more than 300bp at the position of a to-be-detected site on a reference genome, the target region covers the to-be-detected site, and the capture probe is designed for the target region; the number of the to-be-detected sites is one or more; the capture probe specifically binds to the target region. The capture probe disclosed by the invention is high in variation detection sensitivity, generally high in sequencing depth and reliable in result; variation at different positions of different chromosomes can be detected at the same time, and parallel detection is achieved. According to the detection method based on the liquid-phase gene chip, multi-site, multi-sample and cross-species parallel detection can be realized, and single base mutation can be accurately identified.

The present application belongs to the technical field of gene detection and gene molecular breeding, and particularly relates to a meat duck whole genome molecular probe combination based on molecular phenotype screening, a 50K gene chip and application thereof. The present application provides a molecular probe combination of a marker site combination for meat duck whole genome selection and a gene chip, which simultaneously covers 7 representative meat duck breeds, 71 economic traits, has rich polymorphism in a meat duck population, is more targeted, and has lower detection cost and faster speed compared to high-throughput sequencing. The present application mines relevant sites for meat duck growth, feed efficiency, slaughter, reproduction, egg quality and various types of molecular phenotypes, designs a breeding chip, has higher selection accuracy compared to high-throughput sequencing technology, has high breeding value, can be widely applied to genotype detection of meat duck breeding, and has a pioneering significance for meat duck genome selection breeding.

The application provides a reagent combination, a kit and a method for detecting organic sulfur cycle metabolism related genes, and belongs to the field of organic sulfur cycle metabolism related gene detection. The reagent combination comprises a primer pair combination for specifically amplifying organic sulfur cycle metabolism related genes. The reagent combination, the gene chip and the kit of the application have very high sensitivity, specificity and coverage for detecting organic sulfur cycle metabolism related genes. The method of the application is based on HT-qPCR, can simultaneously quantitatively detect multiple environmental samples, can well distinguish different organic sulfur cycle metabolism genes in different environments, and can absolutely quantitatively and relatively quantitatively detect the abundance, provides a high-throughput molecular tool for related research of organic sulfur cycle, effectively supplements the defects of low qPCR detection throughput and inability of absolute quantification of metagenome sequencing, and provides strong technical support for ecological research.

The application belongs to the technical field of gene detection and molecular diagnosis, and particularly relates to a kit for detecting a pathogenic gene of phenylketonuria and application thereof. The kit comprises a primer pair for amplifying specific exons and splicing regions of a phenylalanine hydroxylase gene, and specific probes for detecting hot spot mutations and deletion / repetition variations of the gene. The application can detect genetic variations related to phenylketonuria in one time, quickly and accurately by combining optimized multiplex polymerase chain reaction with high-throughput sequencing or gene chip technology, and covers various known hot spot mutations and copy number variations including c.1222C>T, c.1068-11G>A, c.728G>A and c.1162G>A. The kit has high detection sensitivity and strong specificity, and is suitable for positive recall diagnosis of neonatal phenylketonuria screening, genetic diagnosis of suspected patients, carrier screening and prenatal diagnosis, and provides an efficient tool for precise prevention and control of phenylketonuria.

The invention provides a probe composition, a gene chip, a reagent, a kit and application. The probe composition is designed based on capture areas of 34 genes related to dominant single-gene genetic diseases, can be used for non-invasive prenatal genetics screening, and is suitable for prenatal screening of genetic variation positive family history, bad fertility history, fetal ultrasound examination abnormality, pregnant woman elderly, father elderly and the like. The omission ratio and the birth rate of fetuses suffering from the dominant single-gene hereditary disease are effectively reduced.

This application discloses a high-resolution probe combination, gene chip, kit, and method for detecting probiotics, belonging to the field of microbial detection technology. The probe combination of this application is designed for the entire genome and can simultaneously detect 15 probiotic species, offering higher resolution and sensitivity compared to commonly used PCR methods in the prior art. Simultaneously, the probe combination, gene chip, kit, and method of this application can assess the relative abundance of probiotics, thereby achieving quantitative detection.

The invention discloses a pig SINE-RIP probe combination, a gene chip, a kit and application, the pig SINE-RIP probe combination is based on a reference genome Sscrofa11.1, has wide variety and group applicability, and detects a type of SINE-RIP structure variation markers of SINE-RIP which is large in fragment, easy to cause gene activity change and large in genetic effect. The method is suitable for various applications and researches such as pig genome selective breeding, pig germplasm resource identification and evaluation, genetic relationship analysis, genetic diversity analysis and evaluation, functional gene or locus positioning, whole genome association analysis and the like, the detection process is convenient to operate, the flux is high, the cost is low, the detection rate is high, and the typing result is accurate. The probe combination effectively supplements and even substitutes an existing SNP molecular marker system, the accuracy of genome selection can be further improved, a brand-new high-reliability tool is provided for whole genome related research and application of pigs, and the industrial application value is extremely high.

The application provides a SNP site combination, a molecular probe combination, a zucchini whole genome gene chip and an application thereof. The SNP site combination comprises at least one SNP site in Table 1, and the physical position information of the SNP site in Table 1 is determined based on the alignment of the genomic sequence of the zucchini reference genome 22S20 or an updated version thereof. Specifically, the application provides a zucchini whole genome 10K liquid phase gene chip and an application thereof. The zucchini whole genome 10K liquid phase gene chip can be used for high-depth resequencing of a zucchini target site, and accurate detection of the genotype of the SNP site. Meanwhile, the zucchini whole genome 10K liquid phase gene chip has the advantages of low cost and high efficiency, which helps to speed up the zucchini breeding process and provides strong technical support for the development and utilization of zucchini germplasm resources.

The present application belongs to the technical field of gene chip, and particularly relates to a SNP molecular marker combination and chip related to fresh corn nutritional element traits and application thereof. Through whole genome association analysis, 257 SNP sites significantly associated with fresh corn nutritional elements (K, Mg, Ca, Fe, Zn, Mn, Cu and Ni) are screened, 20,000 SNPs uniformly distributed on the genome are selected in combination with SNPs identified by resequencing data, 21,768 SNP molecular marker combinations shown in Table 2 are obtained, and are tightly linked to fresh corn nutritional element content. Based on the above-mentioned SNP molecular marker combination, a probe is designed, a whole genome chip is constructed, and fresh corn genetic diversity and variety analysis can be carried out. Only by using the chip to identify the genotype of the fresh corn inbred line, the nutritional element content of the fresh corn inbred line can be predicted, and the application has the advantages of clear selection target, early detection and environmental influence.

The invention discloses a mitomycin C drug sensitive marker, a detection reagent and application of the mitomycin C drug sensitive marker, the mitomycin C drug sensitive marker is a mitomycin C drug-resistant marker or / and a mitomycin C sensitive marker, and the mitomycin C drug-resistant marker is one or more of PRODH, ANKRD35, ADAMTSL5, PTPRQ, NOXA1, PRAC1, CRYM, NFIB, SYK or DGAT2; and the mitomycin C sensitive marker is one or more of GABRP (gamma-aminobutyric acid binding protein), PABPC1L (poly (p-aminobutyric acid binding protein 1L) or PODXL2 (peroxidase The mitomycin C drug sensitive marker can be used for predicting the drug effect of mitomycin C. According to the present invention, the expression of the related molecules is detected by sequencing the transcriptome of the tissue of the urothelium carcinoma patient, or by using the PC R, the gene chip, the tissue chip, the NanoString technology, the immunohistochemistry and the ELISA method, such that the clue can be provided for the medication of the mitomycin C and the precise treatment of the bladder cancer.

The invention discloses an image registration method based on a gene chip, scanning equipment and a storage medium, and the method comprises the steps: obtaining a to-be-registered region selected on the gene chip and obtaining a candidate fluorescence image set corresponding to the to-be-registered region, the camera device is controlled to move in different directions, and fluorescence images corresponding to the directions are obtained through shooting; acquiring reference data of the gene chip, and acquiring a reference region fluorescence image matched with the region to be registered from the reference data; calculating a registration index value of each reference region fluorescence image and the candidate fluorescence image set; and based on registration index values of the reference region fluorescence images and the candidate fluorescence image set, determining an offset corresponding to the to-be-registered region so as to correct camera equipment.

The present application relates to the technical field of gene chip, and more particularly to a 5K liquid phase chip for sheep breed identification and application thereof. The 5K liquid phase chip comprises probes of target SNP molecular markers, and the SNP molecular markers are located on 5145 SNP molecular markers on a sheep reference genome Genome assembly ARS-UI_Ramb_v2.0, as shown in Table 1. The liquid phase chip prepared by using the molecular markers can realize genotyping, and has high application value in many fields such as sheep breed identification.

A method of identifying a subject having Kawasaki disease (KD), which includes discriminating the subject from a subject having another condition, for example other infectious and inflammatory conditions, such as those that present similar symptoms to KD. Also provided is a minimal gene signature employed in the method, as well as primers, probes and gene chips for use in the method.