Nucleic acid combined testing kit of respiratory tract infection pathogens

Abstract

The invention discloses a nucleic acid combined testing kit of respiratory tract infection pathogens. The invention develops a set of primer-probe combinations which can detect multiple types of respiratory tract infection pathogens such as novel coronavirus, influenza virus a, influenza virus b, respiratory syncytial virus, human parainfluenza virus, adenovirus, mycoplasma pneumonia and chlamydiapneumonia through combination of a multiple fluorescence quantitative PCR technology and a flow-through hybridization and gene chip technology, wherein nucleotide sequences thereof are shown by SEQ ID NO:1-36 respectively. The nucleic acid combined testing kit of the respiratory tract infection pathogens is established. The kid can realize synchronous combined testing of the 8 respiratory tract infection pathogens, is high in detection accuracy, specificity and sensitivity, good in repeatability, low in false negativity and false positivity, short in detection time and low in cost, can realize comprehensive detection of a patient, can locate a disease source accurately, can realize treatment in time or make corresponding quarantine measures and is of important significance to effective control of respiratory tract infection and subsequent prevention of outbreak of relevant contagion and infection.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and clinical diagnosis. More specifically, it relates to a nucleic acid combined detection kit for respiratory infection pathogens. Background technique [0002] Respiratory tract infection (RTI) is the most common type of human disease, which can occur in any gender, age and region, and is one of the leading causes of morbidity and death worldwide. The clinical symptoms and signs caused by respiratory tract infection are relatively similar, and its clinical manifestations are mainly rhinitis, pharyngitis, laryngitis, tonsillitis and other symptoms, and severe cases can cause tracheitis, bronchitis and pneumonia, etc. The treatment methods, efficacy and course of the disease also vary. It has been proven that most respiratory diseases are caused by pathogens other than bacteria, among which respiratory viruses are the most common. [0003] Common respiratory viruses include influenza virus,...

AI Technical Summary

Problems solved by technology

However, in the clinical detection of respiratory tract infection, single detection of a pathogen of respiratory tract infection is time-consuming, laborious and costly.

At present, multiple detection is mainly based on the antigen or antibody detection of immunological methods, but the sensitivity and specificity of immunological methods are low, and there is a window period in antibody detection, which is easy to miss in the early diagnosis of diseases

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