Alpha-globin gene mutation detection kit as well as preparation method and use thereof

Abstract

The invention provides an alpha-globin gene mutation detection kit as well as a preparation method and a use thereof, and relates to protein gene mutation detection. The alpha-globin gene mutation detection kit is provided with a box, an amplification reagent bottle and a control reagent bottle; the amplification reagent bottle and the control reagent bottle are arranged in the box. The preparation method comprises the following steps: 1) preparing an amplification reagent which comprises an HBA PCR mixed solution and an HBA enzyme mixed solution; 2) preparing a control reagent which comprises HBA standard control and HBA negative control; and 3) putting the amplification reagent prepared in the step 1) and the control reagent prepared in the step 2) in the box, thereby obtaining the alpha-globin gene mutation detection kit. The alpha-globin gene mutation detection kit can be applied to detecting the alpha-globin gene mutation. The alpha-globin gene mutation detection kit is convenient and fast, capable of detecting a plurality of sites by use of one single reaction, short in time consumption, high in detection flux, high in detection specificity, and easy in result interpretation.

Description

technical field [0001] The invention relates to protein gene mutation detection, in particular to an α-globin gene mutation detection kit and its preparation method and application. Background technique [0002] α-globin gene mutations are relatively common in the human genome, and its gene mutations include many types such as single base or a few base conversions, transversions, insertions, and deletions. In the α-globin gene cluster, α1-globin gene and α2-globin gene are two genes with high GC content and high homology, and the polypeptide chain sequences encoded by the two genes are identical, while α-globin gene Protein gene mutations can lead to reduced expression of the encoded α-globin, resulting in changes in a series of biological traits such as α-thalassaemia. Therefore, the detection of α-globin gene mutation has important biological significance. Pan Ganhua et al. (Pan Ganhua, et al. Molecular epidemiological investigation of non-deletion α-thalassemia in Nanha...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a simple and fast, high sensitivity, high reliability, low cost based on A kind of α-globin gene mutation detection kit based on fluorescent PCR melting curve technology and its preparation method and application

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