20results about How to "High detection specificity" patented technology

The application provides a detection method and application of miRNA and analogs thereof, and relates to the field of nucleic acid detection. The application designs probe 1 and probe 2 aiming at target miRNA and miRNA analogs; the probes combine with a part of miRNA respectively, probe 2 can be combined to a solid phase carrier such as a chip or a microsphere, and probe 1 is modified with signal labeling materials such as a fluorescent dye or biotin; after the target miRNA or miRNA analog is mixed with probe 1 and probe 2 and incubated, the probe 2 can not be hybridized with the miRNA analog by optimizing the hybridization temperature, only the target miRNA is hybridized and captured, and the detection specificity is high.

The present application relates to the technical field of gene mutation detection, and particularly relates to a system, method and application for detecting exon skipping mutation.The present application constructs a self-catalytic system based on specific double recognition induction of CRISPR-Cas12a, which is used for sensitive and reliable detection of MET 14 exon skipping mutation of lung cancer patients.The system can effectively release the fluorescence signal through the Cas12a auxiliary cutting, and further realize signal amplification through the self-catalytic mechanism.The present application can solve the technical problems of the existing exon skipping mutation detection system, such as complex detection process, long time consumption, and unsatisfactory detection accuracy.The detection system of the present application has high sensitivity, stability and good reproducibility, and the method has low cost, simple operation, short detection period, good specificity, and low false positive rate and false negative rate.The present application is suitable for clinical sample determination and has an ideal application prospect.

The application provides a porcine parvovirus detection method and kit based on the nature of four-strand DNA oxidoreductase. The method uses isothermal amplification technology to amplify the genomic fragment containing the four-strand DNA sequence in the porcine parvovirus genome, and realizes rapid and visual detection of porcine parvovirus by adding hematin and oxidoreductase substrate for color reaction.

The invention discloses AS-PCR primers and a method for detecting drug resistance of peronophythora litchii to azoxystrobin. An AS-PCR primer capable of being used for detecting whether peronophythora litchii has drug resistance to azoxystrobin or not is obtained by comparing differential sequences of a peronophythora litchii strain capable of resisting azoxystrobin and a peronophythora litchii strain sensitive to azoxystrobin, and a method for detecting the drug resistance of peronophythora litchii to azoxystrobin is constructed based on the AS-PCR primer. The detection specificity is good, and the sensitivity is high. The AS-PCR primer and the detection method are used for amplifying a to-be-detected strain, and if a single band with the consistent size can be obtained, the detected strain is anti-azoxystrobin peronophythora litchii. Compared with a traditional detection method, the detection method disclosed by the invention is short in time consumption, simple, convenient and feasible, is beneficial to quickly monitoring early epidemic of peronophythora litchii anti-azoxystrobin groups, and provides scientific guidance for scientific prevention and control of peronophythora litchii.

This invention discloses a pyrimethanil hapten, an artificial antigen, and their preparation methods and applications. The structural formula of the pyrimethanil hapten is shown below. The arm introduced in this invention not only possesses an active group but also fully retains the benzene ring and dimethylpyrimidine ring structure of the target analyte, enhancing the recognition site. The pyrimethanil artificial antigen and monoclonal antibody of this invention exhibit high specificity for ELISA detection, with an IC50 rating of [missing information]. 50 The value is 1.97 μg / L, which is better than existing monoclonal antibodies. It can be used in colloidal gold immunochromatography to rapidly and conveniently detect pyrimethanil. The colloidal gold immunochromatographic test strip prepared in this invention has a detection sensitivity of 10 μg / L for pyrimethanil.

The application discloses a method, system and device for detecting Tau protein based on machine learning of surface enhanced Raman spectroscopy, and the method comprises the following steps: S1, constructing a SERS signal probe with minimum initial Raman signal; S2, mixing a sample to be detected with the SERS signal probe, and collecting SERS spectra of the mixed system by using a Raman spectrometer; S3, pre-processing the collected SERS spectra, wherein the pre-processing comprises baseline correction, feature peak identification and minimum-maximum normalization processing in sequence, so as to obtain standardized spectral data; and S4, inputting the standardized spectral data into a machine learning regression model which is trained in advance, and automatically outputting a concentration value of Tau protein in the sample to be detected based on the input spectral characteristics by the machine learning regression model. The application can quantitatively detect phosphorylated Tau protein quickly, accurately and with low detection limit under the premise of keeping high specificity, and has high clinical conversion potential.

This invention discloses chloramphenicol hapten, antigen, antibody, colloidal gold detection device, and their preparation methods and applications. The chloramphenicol hapten prepared by this invention using a total synthesis method retains all potential recognition sites of chloramphenicol to the maximum extent, and in particular, for the first time retains the most critical dichloro structure of chloramphenicol. After introducing a spacer arm, its electron cloud distribution is almost identical to the overall electron cloud distribution of chloramphenicol, improving the immunogenicity of the chloramphenicol antigen. The chloramphenicol antigen and monoclonal antibody prepared by this invention have high specificity for ELISA detection, with an IC50 value of [missing information]. 50 The value was 8.21 pg / mL, the linear detection range was 1.71–39.45 pg / mL, the cross-reactivity to common structural analogs was less than 0.01%, and the sensitivity to chloramphenicol in the sample was 0.1 µg / kg.

The invention discloses a fluorescence analysis and detection method and system based on quantum dots, and belongs to the technical field of analysis and detection. According to the method, functional modified quantum dots are used as fluorescent probes, a fluorescence detection system is constructed through specific recognition reaction, and high-sensitivity and high-specificity detection of a target detection object is realized by combining collection and quantitative analysis of fluorescence signals. The corresponding system comprises an excitation light source module, a sample reaction module, a fluorescence detection module, a data processing module and a control module, all the modules work cooperatively, key parameters in the detection process can be precisely regulated and controlled, and the detection precision and stability are improved. The method solves the problems of poor probe stability, low quantum yield and high detection limit in a traditional fluorescence detection method, has the advantages of high detection speed, high specificity and wide application range, and can be widely applied to the fields of food safety detection, environmental pollutant monitoring, biomedical diagnosis and the like.

This invention relates to immunological detection, particularly to the field of immunological diagnosis of tumors. Specifically, this invention provides a method for diagnosing nasopharyngeal carcinoma based on anti-EBV antibody levels, and a kit for using said method. This invention also provides a polypeptide encoded by the EBV BNLF2b gene for the above-mentioned diagnosis and its use in the diagnosis of nasopharyngeal carcinoma.

The application relates to the field of tumor biotechnology and discloses a long-chain non-coding RNA molecular marker combination and application thereof. The long-chain non-coding RNA molecular marker combination comprises seven molecules, including ENSG00000233397, BARX1-DT, LSAMP-AS1, HOXB-AS4, MNX1-AS1, LINC01385 and AL513318.2; the seven long-chain non-coding RNAs are significantly highly expressed in laryngeal squamous cell carcinoma tissues, each molecule can promote the proliferation, migration and invasion ability of laryngeal squamous cell carcinoma cells, and is positively correlated with the poor prognosis of laryngeal squamous cell carcinoma. The long-chain non-coding RNA molecular marker combination is applied to the preparation of a laryngeal squamous cell carcinoma auxiliary prognosis evaluation kit and a preparation for treating laryngeal squamous cell carcinoma; the laryngeal squamous cell carcinoma auxiliary prognosis evaluation kit adopts real-time fluorescent quantitative PCR to detect the expression amount of the seven long-chain non-coding RNAs, and combines the seven expression amount results to conveniently and efficiently judge the disease condition and prognosis evaluation of a laryngeal cancer patient; and the preparation for treating laryngeal squamous cell carcinoma comprises at least one or seven of the seven long-chain non-coding specific interference RNAs.

The invention relates to a low-altitude water area biochemical toxicity monitoring system and method. The monitoring system comprises an unmanned aerial vehicle platform and a ground monitoring terminal. The unmanned aerial vehicle platform comprises an unmanned aerial vehicle, a controllable folding and unfolding mechanism and a detection unit, and the controllable folding and unfolding mechanism is arranged at the abdomen of the unmanned aerial vehicle; the winding and unwinding mechanism is connected with the detection unit through a winding and unwinding cable, the detection unit is controlled to ascend and descend through the winding and unwinding mechanism, and then the underwater depth of the detection unit is controlled; a signal processing and wireless transmitting unit is arranged in the unmanned aerial vehicle, the ground monitoring terminal is provided with a wireless receiving unit, and the ground monitoring terminal receives wireless signals of the unmanned aerial vehicle, analyzes the wireless signals and displays monitoring results. The optical fiber sensor probe of the modified specific biochemical recognition membrane is integrated with the unmanned aerial vehicle platform, high-precision detection capability is delivered to a target water area by utilizing the maneuverability of the unmanned aerial vehicle, and in-situ and real-time monitoring of low-altitude water area biochemical toxicity is realized.

This invention relates to a double-antibody sandwich ELISA kit for detecting serum PTGS1 protein, comprising: a PTGS1 capture antibody, an unlabeled PTGS1 detection antibody, an enzyme-labeled IgG secondary antibody, a solid-phase carrier, an enzyme substrate, a blocking buffer, a washing buffer, and a stop solution; the IgG secondary antibody specifically binds to the PTGS1 detection antibody; the PTGS1 capture antibody is a rabbit monoclonal antibody that specifically binds to the linear epitope A of human PTGS1 protein, the amino acid sequence of which is SEQ ID NO:1, corresponding to amino acids 271-282 of human PTGS1 protein; the PTGS1 detection antibody is a mouse monoclonal antibody that specifically binds to the B epitope of human PTGS1 protein; the amino acid sequences of the B epitope at the A epitope do not overlap and do not constitute steric hindrance. This kit avoids the conserved homologous region between PTGS1 and its homologous protein PTGS2, ensuring the accuracy of the capture antibody recognition, and uses an indirect detection mode with enzyme-labeled goat anti-mouse IgG secondary antibody to achieve signal cascade amplification, significantly improving detection sensitivity.

The application discloses a kit for detecting cat feces related circular virus and application thereof, and belongs to the technical field of DNA virus detection. The detection kit can significantly improve the detection flux and timeliness of the cat feces related circular virus, has high detection sensitivity and strong specificity, can effectively solve the problem of low FeSCV virus load in healthy cats and easy missed detection, has clear components, low device dependency, and the color change can be directly observed by naked eyes under natural light to read the result, greatly simplifies the operation process, reduces the personnel operation error, and improves the reliability of the detection result, has low requirement on professional knowledge, is particularly suitable for use in primary pet clinics, cat houses / farms, isolation quarantine areas and field epidemiological investigation, can simultaneously detect multiple geographical strains of FeSCV such as Chinese epidemic strains and Japanese reference strains, can realize consistent detection of different geographical strains, and provides a reliable tool for the epidemiological monitoring of FeSCV.

The present application relates to the field of electrochemical analysis and detection, and discloses a method for detecting mycotoxin based on CRISPR-Cas12a system, comprising: (1) reacting single-stranded DNA with recognition DNA of mycotoxin to obtain single-stranded-recognition DNA complex, wherein the single-stranded DNA is complementary to the recognition DNA; (2) mixing the single-stranded-recognition DNA complex and the sample to be tested which may contain mycotoxin to obtain reaction mixture I after reaction II, and mixing the reaction mixture I with auxiliary DNA containing activated DNA fragments to obtain reaction mixture II after reaction III, wherein the auxiliary DNA is complementary to the single-stranded DNA; (3) mixing the reaction mixture II with CRISPR-Cas12a system solution to obtain reaction solution after activation; (4) adding the reaction solution dropwise to an electrochemical biosensor to perform reaction V, and detecting the electrochemical signal of the electrochemical biosensor after reaction V is completed. The method has high sensitivity and high selectivity, and is simple and rapid to operate.

The invention relates to computing (S101) an information content of a section of a current image of a series of images of a video signal, wherein the video signal has to be fed to an algorithm for computing and indicating a detection of an object in the video signal. If the computed information content of the section of the current image is less than a threshold (S103), the computing and indicating of the detection of an object of the section of at least the current image or of the current image and further images of the series of images is suppressed (S105).

This invention belongs to the field of chloride detection technology, specifically relating to a perovskite-based nanomaterial, its preparation method, and its application. The perovskite-based nanomaterial includes upconversion nanomaterials and Ag₂S quantum dots, with the upconversion nanomaterials and Ag₂S quantum dots linked by chemical bonds. The upconversion nanomaterials have a core-shell structure, with Yb 3+ / Er 3+ The perovskite-based nanomaterials form the core, which is then coated with a Cs₂NaYF₆ shell. The perovskite-based nanomaterials are added to the sample, and the emission intensity of the sample is measured in the 300–750 nm range under 980 nm laser excitation. The chloride concentration is calculated based on a standard curve. The perovskite-based nanomaterials prepared in this invention exhibit high detection accuracy in chloride detection, enabling quantitative detection of chloride at the micromolar level. Furthermore, the preparation method is simple and inexpensive.

The invention relates to the technical field of biological detection, and discloses a primer, a probe and a kit for detecting aeromonas hydrophila based on fluorescent PCR (Polymerase Chain Reaction) and application of the primer, the probe and the kit. A primer and fluorescent probe combination is obtained by designing and screening a specific gene conserved region of aeromonas hydrophila. The specific amplification of the aeromonas hydrophila gene based on the fluorescent PCR technology can be successfully realized. According to the method, the detection accuracy and sensitivity are improved, the aeromonas hydrophila can be detected in the early stage of bacterial septicemia of silver carps and bighead carps, prevention and control of bacterial septicemia of silver carps and bighead carps are facilitated, and the basic requirements of field detection of farms can be met.

The invention belongs to the technical field of biological detection, and provides an RPA-LFD (recombinase polymerase amplification-lateral flow dipstick) visualization method for detecting fluke fasciatus infection of cattle and sheep. According to the invention, specific forward and reverse primers and probes are designed on the basis of the 18S-ITS1 gene sequence of fasciola hepatica or the highly specific region and conserved region sequences of fasciola hepatica. The RPA-LFD method established by the invention is high in detection sensitivity, good in specificity and stable in detection effect, and has no cross reaction with common intestinal parasites of cattle and sheep, including schistosoma japonicum and the like; the lowest detection limit of the genome reaches 100 fg / mu L, and the lowest detection limit of the standard plasmid reaches 1.93 * 10 < 1 > copies / mu L. The detection can be completed within 30 minutes, complex and special instruments and equipment are not needed, the time consumption is short, the operation is simple, rapid and accurate, on-site portable visual rapid detection can be realized, and the kit has clinical application value in the aspects of diagnosis and comprehensive prevention and control of cattle and sheep fluke fasciata infection.

The application belongs to the field of biology and particularly relates to a composition of a biosensor for detecting APE1, a working solution and a product, the composition comprising a double-stranded promoter, an H1 hairpin, an H2 hairpin and an FQ reporter molecule, the double-stranded promoter comprising a promoter I and a blocking chain B, and the FQ reporter molecule comprising a RepF chain and a RepQ chain, the biosensor formed by the composition of the application has high sensitivity and strong specificity for detecting APE1.

This invention provides extracellular vesicle miRNAs from milk for detecting heat stress in dairy cows and their applications, belonging to the field of dairy cow health technology. This invention, through the isolation, extraction, and miRNA sequencing of extracellular vesicles derived from dairy cow milk, discovered specific miRNA molecules closely related to heat stress. Using the miRNA unconservative_3_49980 from these extracellular vesicles as a biomarker, this invention provides a simple, non-invasive, and stress-free method for detecting heat stress in dairy cows with high sensitivity. This invention also designs specific primer pairs for the miRNA, enabling the construction of a kit for early, non-invasive assessment of heat stress in dairy cows.