817results about How to "High detection specificity" patented technology

The disclosure provides methods and apparatus of particular benefit for patients suffering heart failure including cardiac dysfunction, chronic HF, and the like and all variants thereof. According to the disclosure monitoring and therapy delivery for a wide variety of acute and chronic cardiac dysfunctions are described and depicted. Various forms of paired or coupled pacing therapy delivery provided alone or in combination with neurostimulation therapy delivered by both implantable and external apparatus, including defibrillation therapy are also provided herein.

Techniques are described for detecting and distinguishing among ischemia, hypoglycemia or hyperglycemia based on intracardiac electrogram (IEGM) signals. In one technique, these conditions are detected and distinguished based on an analysis of: the interval between the QRS complex and the peak of a T-wave (QTmax), the interval between the QRS complex and the end of a T-wave (QTend), alone or in combination with a change in ST segment elevation. By exploiting QTmax and QTend in combination with ST segment elevation, changes in ST segment elevation caused by hypo / hyperglycemia can be properly distinguished from changes caused by cardiac ischemia. In another technique, hyperglycemia and hypoglycemia are predicted, detected and / or distinguished from one another based on an analysis of the amplitudes of P-waves, QRS-complexes and T-waves within the IEGM. Appropriate warning signals are delivered and therapy is automatically adjusted.

Techniques are described for detecting and distinguishing among ischemia, hypoglycemia or hyperglycemia based on intracardiac electrogram (IEGM) signals. In one technique, these conditions are detected and distinguished based on an analysis of: the interval between the QRS complex and the peak of a T-wave (QTmax), the interval between the QRS complex and the end of a T-wave (QTend), alone or in combination with a change in ST segment elevation. By exploiting QTmax and QTend in combination with ST segment elevation, changes in ST segment elevation caused by hypo / hyperglycemia can be properly distinguished from changes caused by cardiac ischemia. In another technique, hyperglycemia and hypoglycemia are predicted, detected and / or distinguished from one another based on an analysis of the amplitudes of P-waves, QRS-complexes and T-waves within the IEGM. Appropriate warning signals are delivered and therapy is automatically adjusted.

The present invention relates to an automatable method for obtaining an improved diagnosis of cancer, and its precancerous stages in uterine cervical smears by simultaneously staining and detecting at least two different molecular markers, which exhibit a disease-associated change in gene expression, in a cell by means of using antibodies or nucleic acid probes.

Systems and methods employing a weighted zero crossing sum metric (WZCSM) derived from the EGM that improves the specificity of discriminating between a monomorphic tachyarrhythmia and a polymorphic tachyarrhythmia are provided that examine frequency content and baseline information of the EGM as discriminatory signatures are disclosed. In preferred embodiments, high rate polymorphic QRS complexes are discriminated from high rate monomorphic QRS complexes to increase the specificity of detection of polymorphic VT and VF. Zero crossing points (ZCPs) and weighted ZCP slopes of the high pass filtered EGM signal in baseline and sense event windows are identified. The weighted ZCPs of the baseline window are summed to provide a baseline WZCSB, and the weighted ZCPs of the VSENSE event window are summed to provide a VSENSE event WZCSE. A WZCSM is derived from the VSENSE event WZCSE and the baseline WZCSB that is compared to a threshold.

Methods and apparatus are provided for discriminating high rate polymorphic QRS complexes from high rate monomorphic QRS complexes to increase the specificity of detection of polymorphic VT and VF employing wavelet transform signal processing of the QRS complexes are disclosed. Wavelet transforms are applied to the sampled amplitude values of a sequence of QRS complexes to develop wavelet transform coefficient (WTC) data sets. At least selected ones of the WTC data sets are processed and comparisons are made to determine a wavelet match score. A determination is made as a function of the wavelet match scores of the series of successive QRS complexes that characterizes the most recent QRS complex as more or less likely to signify polymorphic VT or VF.

A method for improving the sensitivity of an immunoassay by a factor of at least 10 to about 25, and perhaps greater. Furthermore, in addition to increasing the sensitivity of the assay, the method of this invention also improves assay specificity. In order to match or improve upon sensitivity of 0.2 pg / mL for an analyte, this invention provides an immunoassay involving amplification of a signal, e.g., a chemiluminescent signal, a specific binding member, e.g., a monoclonal antibody, and microparticle separation, e.g., magnetic microparticle separation, from large volumes of sample (e.g., from about 0.2 to about 3 mL). Such an immunoassay can be carried out with an automated immunoassay analyzers, e.g., an automated immunoassay analyzer in the ARCHITECT® family of analyzers.

A computer-implemented method for determining and characterizing, which portions or shapes of a medical image correspond to a shape of interest is provided. A candidate shape is obtained after which a visible surface is computed adjacent to this candidate shape. A visible surface includes one or more portions of the medical image that are visible by the candidate shape. Once the visible surface is determined, parameters of the visible surface are computed. Then the method further includes the step of determining whether the candidate shape corresponds to a shape of interest. The method further includes the step of computing features of the candidate shape and / or classifying the candidate shape. The advantage of the computer-implemented method is that it provides a high detection specificity, i.e. reducing false positives, without sacrificing sensitivity of the detection of a shape of interest.

In one aspect of the present invention, a method for calculating a response value at a first voxel indicative of a global shape in an image is provided. The method includes the steps of (a) determining at least one local shape descriptor associated with each of the at least one local shape descriptor; (b) determining a spread function associated with the each of the at least one local shape descriptor; (c) determining second voxels around the first voxel; (d) calculating values for each the at least one local shape descriptor at each of the second voxels; (e) determining a contribution of each of the second voxels at the first voxel based on the spread functions; and (f) using a combination function to combine the contributions to determine the response value indicative of the global shape.

Techniques are described for adaptively adjusting detection thresholds for use in detecting cardiac ischemia and other abnormal physiological conditions based on morphological parameters derived from intracardiac electrogram (IEGM) signals, impedance measurements, or other signals. In one example, where ST segment elevation is used to detect cardiac ischemia, default detection thresholds are determined in advance from an examination of variations in ST segment elevations occurring within a population of patients. Thereafter, an individual pacemaker or other implantable medical device uses the default thresholds during an initial learning period to detect ischemia within the patient in which the device is implanted. During the initial learning period, the pacemaker also collects data representative of the range of variation in ST segment elevations occurring within the patient. The pacemaker then adaptively adjusts the thresholds based on the range of variation so as to improve detection specificity within the patient.

The invention discloses a test strip which is used for testing porcine viral diarrhea pathogen in one step, and the test strip comprises a support layer, an adsorption layer and a protection layer. The adsorption layer has a sample fiber layer, a gold antibody fiber layer, a cellulose film layer and a hygroscopic material layer at the handle end sequentially from a test end. The cellulose film layer has a test blot and a contrast blot, and the gold antibody fiber layer is adhered with at least one of monoclonal antibodies or polyclonal antibodies of anti-TGEV, anti-PDEV and anti-RV with colloid gold mark; the test blot is printed by at least one of polyclonal antibody liquid or monoclonal antibody liquid of anti-TGEV, anti-PDEV and anti-RV, and the contrast blot is printed by IgG solution of sheep or rabbit anti-mouse or IgG solution of sheet or rabbit anti-porcine. When used for testing, the test strip has the advantages of strong specificity, high sensitivity, convenient and rapid operation and direct and accurate testing result. The test strip is especially suitable for fast identifying and diagnosing on site.

Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA-RNA hybrids which can be detected by a variety of methods.

The invention provides a microbial marker of colorectal cancer and application of the marker. The microbial marker comprises Faecalibacterium, Streptococcus and Fusobacterium. The microbial marker ofcolorectal cancer has high precision of predicting the risk of colorectal cancer and high sensitivity, only the abundance of the microbial marker of colorectal cancer needs to be obtained, a risk early warning is given out through model calculation, and the possibility of getting colorectal cancer is evaluated. The microbial marker can be used for preventing colorectal cancer, warning a subject and giving the subject a prompt about whether it is necessary to further conduct diagnosis confirmation or not, and is also conductive for individuals to improve the intestinal microbial environment byadjusting diet or medical treatment, so that the risk of getting colorectal cancer is reduced for the individuals.

A wearable cardioverter defibrillator (“WCD”) system includes a support structure that can be worn by a patient, and a defibrillator coupled to the support structure. An ECG input, rendered from an ECG of the patient, may meet a primary shock criterion. One or more sensor modules are further provided, which are worn by the patient at different times. The sensor modules may monitor different physiological parameters of the patient, and transmit signals about them. The WCD system further has a multi-sensor interface to receive the transmitted signals, and a processor to determine from them whether a secondary shock criterion is met. If both the primary and the secondary shock criteria are met, the decision is to shock. The signals increase specificity of the detection, while the patient can wear different modules depending on context.

The invention discloses a method for detecting nucleic acid by a colloidal gold chromatography technology and a reagent kit, and belongs to the technical field of medical biology. A universal test paper strip for nucleic acid detection is provided; colloidal gold particles are marked on a universal probe 1, and then, the universal probe is fixed on a glass cellulose membrane; the probe sequence is designed into a universal sequence; the test paper strip is fixed on a PVC (polyvinyl chloride) bottom plate; a sample pad, glass fiber, an NC membrane and water absorption paper are in sequential arrangement from the left side to the right side, wherein a T line (detection line) and a C line (quality control line) are arranged on the NC membrane; antibodies to labels b cover the detection line; antibodies to labels a cover the quality control line; the specificity probe A series and the specificity B series successfully combine a gold mark probe and nucleic acid amplification fragments in series; the specificity detection of nucleic acid amplification fragments is realized. The method has the advantages that the technical requirement on the experiment personnel is low; the required detection time is short; special instrument equipment is not needed; the popularization to basic levels and remote rural area medical institutions is easy.

The invention discloses an intestinal-tract-derived flora for diagnosing sarcopenia and use of the intestinal-tract-derived flora, and belongs to the biomedicine field. The flora contains Oscillibacter and Eubacterium_brachy_group. By detecting intestinal flora markers, the risk that a subject suffers from the sarcopenia can be evaluated, and auxiliary references are provided for diagnosis and treatment of diseases.

The invention provides a tumor exosome nanometer fluorescence sensor based on magnetic separation and DNA self-assembling. The sensor comprises a functionalized magnetic bead coupled with a GPC-1 antibody, a trigger chain, and three stem circular nucleic acid probes. According to the invention, quantitative detection of the GPC-1(+) exosome subgroup of the breast cancer is realized; and the detection specificity and sensitivity are high and the detection range is wide. The clinical application value of the method is evaluated by detecting the breast cancer GPC-1(+) exosome subgroup in the clinical plasma source specimen. The method having high universality not only can be applied to other biomarkers with specific aptamers screened out but also can be applied to detection of other exosome subgroups, proteins, cells and metal ions and the like.

The inventive subject matter relates to a method for the detection and diagnosis of Rickettsia prowazekii infection by measuring the increased or decreased expression of specific human genes following infection by microarray or polymerase chain reaction analysis. Gene modulation profiles can be further analyzed by computer. The method permits the early detection and diagnosis of Rickettsia prowazekii exposure and infection and diagnosis of epidemic typhus earlier than any currently available methods.

The invention relates to a technology for detecting nucleic acid and provides a nucleic acid detecting method having the advantages of high sensitivity, high accuracy, low cost, simple operation and quickness, and a nucleic acid film chromatographic hybrid strip thereof. The nucleic acid film chromatographic hybrid strip comprises a sampling pad, target nucleic acid sequence capturing probes, a chromatographic film, a water absorbent pad and a back lining, wherein the chromatographic film is bonded to the middle of the back lining; the sampling pad and the water absorbent pad are bonded to both ends of the chromatographic film respectively; and the target nucleic acid sequence capturing probes are fixed on the chromatographic film between the sampling pad and the water absorbent pad in a straight line. In the method, the colored fluorescence marking probes and the film chromatography technology are combined, the type of a template of a sample can be differentiated by two parameters, namely fluorescence and a physical position, so that the quick genotyping detection of nucleic acid is realized. The nucleic acid detecting method is high in sensitivity, and the detection sensitivity reaches 101 copies / reaction. The nucleic acid detecting method is high in specificity, the high specificity are jointly guaranteed by the primers, detection probes and capturing probes, so that the detection result is more stable and accurate.

The invention relates to a method for testing the total antibody of hepatitis C virus (HCV), based on dual-antibody interlayer method of small-molecule indirect mark, wherein said method is characterized in that: it uses small molecule matter as mark; uses dual-antigen interlayer method to test the total antibody of sample; and said method comprises: using small molecule material to mark HCV antigen; using HCV antigen to pack solid material; using single generate material to mark small molecule antibody or other material that combined with small molecule; the HCV antibody of sample can be reacted with HCV antigen of solid material surface and small molecule mark, to form dual-antigen interlayer composite; then reacting the small molecule with single report molecule, to make the composite with detected signal generate material. The invention can keep activity of antigen, with signal amplifying function and better specificity of dual-antigen interlayer mode, while it can detect variable kinds of antibodies, to improve the detecting sensitivity and specificity.

The invention relates to a method and its medium box for quantity measuring peculiar analysis substance by single capture medium. The method comprises the following steps: combining the tested analysis substance with solid phase capture medium, labeling analysis substance which has been captured by capture medium with report molecule, cleaning-up analysis substance from compound, recombining tested analysis substance with new solid phase capture medium, ascertaining content of analysis substance by report molecule's label signal. The medium box comprises capture device, testing device report molecule used as label and analysis substance eluent.

The invention belongs to the field of biotechnology and clinical molecular diagnosis and in particular relates to a fluorescent genotyping detection kit and a detection method for eight human K-ras gene mutations. According to the method, an MGB (minor groove binder) probe technology is combined with a mismatch ARMS (amplification refractory mutation system) technology, an MGB probe complementary with a DNA (deoxyribonucleic acid) plus strand and an ARMS downstream primer complementary with a DNA minus strand are designed at allelic gene loci, a mismatch locus is introduced in the ARMS downstream primer, the MGB probe is overlapped with the ARMS primer by not more than 5 bp, and the eight human K-ras gene mutations are subjected to genotyping detection by four reaction pipes based on multiple fluorescent PCR (polymerase chain reaction). The method has the advantages of good specificity, high sensitivity, simplicity and quickness for operation, accuracy for genotyping, simplicity for result interpretation and the like, and can be used for detecting the clinical K-ras gene mutations and helping doctors screen the crowed with effective anti-EGFR (epidermal growth factor receptor) treatment.

The invention discloses a latex-enhanced immunoturbidimetric assay kit for neutropil gelatinase-associated lipocalin, and a preparation method thereof. Polyethylene glycol hexamine is selected as theblocking agent of an antibody binding latex particle. The kit used for latex-enhanced immunoturbidimetric assay of the NGAL has the advantages of high detection specificity, high sensitivity and goodstability, and can efficiently detect the NGAL content in urine, plasma and serum, and the detection result is well associated with fluorescence immunochromatography and enzyme linked immunosorbent assay.

The invention provides a primer, a probe, a reagent kit and a method for detecting mutation of BRAF gene V600E on the basis of real-time fluorescent quantitative PCR (polymerase chain reaction) technical platform. The primer, the probe, the reagent kit and the method are quick and low in cost and have clinic detection popularization value.

The invention provides a method for detecting Vibrio parahaemolyticus. The sequence of the forward primer is SEQ ID NO:1, the sequence of the reverse primer is SEQ ID NO:2, and the sequence of the probe is SEQ ID NO:3. A real-time RPA (recombinase polymerase amplification) process is utilized to detect the food-borne pathogen Vibrio parahaemolyticus, thereby implementing safe, specific, quick, sensitive and simple field quick detection on the Vibrio parahaemolyticus, and further overcoming the defects in the existing traditional detection technique. The method is suitable for field detection, can effectively inhibit the epidemic situation caused by Vibrio parahaemolyticus food poisoning in time, and perfects the food safety control system.

The invention discloses a time-resolved fluorescence immunoassay reagent kit which is used for detecting quinoxalinone-2-carboxylic acid and a detection method of the time-resolved fluorescence immunoassay reagent kit. The reagent kit comprises a porous coated plate, a buffer solution, quinoxalinone-2-carboxylic acid standard, freeze-dried antibodies of quinoxalinone-2-carboxylic acid, europium-marked goat anti mouse antibodies, a cleaning solution and an enhancement solution. By measuring fluorescence intensity of counts per second (cps), the content of quinoxalinone-2-carboxylic acid in samples is calculated by a standard curve. The kit provided by the invention has the advantages of simple structure, easiness in assembly, corrosion resistance, light weight, low cost, wide application and the like. A valve core has good centering performance and can move flexibly and reliably and can play a good water-plugging role.

The invention discloses a real-time fluorescence quantitative PCR(Polymerase Chain Reaction) detection kit and related primers for thalassemia genes. The kit comprises more than one pair of the following primers: SEQ ID NO.1 and SEQ ID NO.2 aiming at TATA kits nt-28 (A->G) (-28M), -29 (A->G) (-29M) and CAP-40-43 (AACC) (CAPM) genotypes; SEQ ID NO.3 and SEQ ID NO.4 aiming at CD14-15 (+G) (14-15M), CD17 (A->T) (17M) and the initiation codon mutant ATG->AGG(IntM) genotypes; SEQ ID NO.5 and SEQ ID NO.6 aiming at CD26 (G->A)(betaEM) CDs27-28+C(27-28M), IVS1-1(G->T)(IVS1-1M), IVS1-5(G->C)(IVS1-5M) genotypes; SEQ ID NO.7 and SEQ ID NO.8 aiming at CDs41-42-TCTT (41-42M), CD43G->T(43M), 41-42M / 41-42M genotypes; SEQ ID NO.9 and SEQ ID NO.10 aiming at CD71-72(+A) (71-72M) genotypes; SEQ ID NO.11 and SEQ ID NO.12 aiming at IVS2-654C->T(654M) and 654M / 654M genotypes; and SEQ ID NO.13 and SEQ ID NO.14 aiming at constant spring (CS), Quong Sze (QS) and Westmead (WS) genotypes. The kit has the advantages of good detection specificity and high detection sensitivity.