45results about How to "High detection specificity" patented technology

The invention discloses a human IL-13 antibody, an ELISA kit containing the human IL-13 antibody and a detection method of the human IL-13 antibody. Specifically, the ELISA kit provided by the invention comprises a pair of anti-human IL-13 monoclonal antibodies which are respectively used as a coating antibody and a detection antibody in a double-antibody sandwich ELISA method. The kit can be used for successfully completing quantitative detection of human IL-13, has relatively high detection sensitivity and good detection specificity, can be used for detecting a plurality of species of IL-13, and has good development and application prospects.

The invention provides a kit and a method for simultaneously detecting 11 pathogens of a urogenital system and application, and belongs to the field of molecular biology. The primer group is used for detecting urogenital tract pathogens, and the urogenital tract pathogens comprise ureaplasma urealyticum (UU), chlamydia trachomatis (CT), mycoplasma genitalium (MG), ureaplasma parvum (UP), mycoplasma hominis (MH), adenovirus (ADV), trichomonas vaginalis (TV), herpes simplex virus type I (HSV-I), herpes simplex virus type II (HSV-II), haemophilus influenzae (HI) and neisseria gonorrhoeae (NG). The primer group is used for multi-joint detection of non-gonococcal urethritis related pathogens, has high specificity and sensitivity, is adaptive to non-invasive urine specimens, and supports home self-sampling. The kit is used for clinical diagnosis and can quickly judge pathogen types. Precise medication is performed based on a detection result, the healing time of a patient is shortened, the burden of a doctor is relieved, and meanwhile the pathogen transmission risk is reduced.

The invention discloses a kit for detecting HLA-B27 and a detection method. The kit for detecting HLA-B27 comprises a PCR (Polymerase Chain Reaction) solution, a primer probe mixed solution, a positive reference substance and a negative reference substance, the primer probe mixed solution comprises an upstream primer 1F, a downstream primer 1R and a probe 1P for detecting the HLA-B27, and an upstream primer 2F, a downstream primer 2R and a probe 2P for detecting a reference gene. A fluorescent PCR-melting curve method is adopted, sequence alignment is carried out according to an NCBI database, primers and probes which are high in specificity and capable of simultaneously detecting main subtypes (HLA-B27: 02, HLA-B27: 04, HLA-B27: 05, HLA-B27: 06, HLA-B27: 07 and HLA-B27: 24) of the HLA-B27 are selected, and compatibility detection of different subtypes of the HLA-B27 can be achieved through a pair of primers and a probe. The Tm value in the melting curve instead of the Ct value of the amplification curve is adopted for result judgment, qualitative accuracy is achieved, and the result is visual and easy to interpret.

The invention relates to the technical field of biological detection, in particular to a nucleic acid sequence combination for detecting cervical cell gene methylation, a kit and application. The nucleic acid sequence combination for detecting the methylation of the cervical cell gene, provided by the invention, can specifically detect the methylation of ABCC9 and LINC00605 genes of an endometrial cancer cervical exfoliated cell sample, which are different from those of a healthy human cervical exfoliated cell sample, and the kit for detecting the methylation of the cervical cell gene, which is prepared by utilizing the nucleic acid sequence combination, can be used for detecting the methylation of the cervical cell gene. The kit is applied to diagnosis / screening of endometrial cancer, has relatively high detection sensitivity and specificity on stage I and stage II endometrial cancer, is simple to operate and beneficial to popularization and application, and noninvasive material drawing is realized.

The application provides a detection method and application of miRNA and analogs thereof, and relates to the field of nucleic acid detection. The application designs probe 1 and probe 2 aiming at target miRNA and miRNA analogs; the probes combine with a part of miRNA respectively, probe 2 can be combined to a solid phase carrier such as a chip or a microsphere, and probe 1 is modified with signal labeling materials such as a fluorescent dye or biotin; after the target miRNA or miRNA analog is mixed with probe 1 and probe 2 and incubated, the probe 2 can not be hybridized with the miRNA analog by optimizing the hybridization temperature, only the target miRNA is hybridized and captured, and the detection specificity is high.

The present invention relates to an HPV nucleic acid detection kit and a preparation method and application thereof. The inventor designs a dual probe detection combination of a U probe and a B probe designed for an HPV gene sequence, and based on this, develops a multiple nucleic acid detection kit and a PCR melting curve method for detecting multiple targets in a single tube. By introducing an L-type primer and a universal primer system: the uniformity of amplification is ensured; the difference between different amplicons in the amplification product caused by the difference in amplification efficiency between specific primers is eliminated. This method has a short operation time, and the detection can be completed within 3 hours. The lid does not need to be opened during the detection process, which can reduce PCR product contamination, and the detection specificity is high, which can reduce nonspecific amplification in the PCR reaction, improve the methodological detection sensitivity, and the result is easy to interpret.

The present application relates to the technical field of gene mutation detection, and particularly relates to a system, method and application for detecting exon skipping mutation.The present application constructs a self-catalytic system based on specific double recognition induction of CRISPR-Cas12a, which is used for sensitive and reliable detection of MET 14 exon skipping mutation of lung cancer patients.The system can effectively release the fluorescence signal through the Cas12a auxiliary cutting, and further realize signal amplification through the self-catalytic mechanism.The present application can solve the technical problems of the existing exon skipping mutation detection system, such as complex detection process, long time consumption, and unsatisfactory detection accuracy.The detection system of the present application has high sensitivity, stability and good reproducibility, and the method has low cost, simple operation, short detection period, good specificity, and low false positive rate and false negative rate.The present application is suitable for clinical sample determination and has an ideal application prospect.

The application provides a porcine parvovirus detection method and kit based on the nature of four-strand DNA oxidoreductase. The method uses isothermal amplification technology to amplify the genomic fragment containing the four-strand DNA sequence in the porcine parvovirus genome, and realizes rapid and visual detection of porcine parvovirus by adding hematin and oxidoreductase substrate for color reaction.

The present invention discloses a primer-probe composition for detecting the heat laelaps of honey bees and a detection method thereof. Specifically disclosed is a primer pair for detecting the heat laelaps of honey bees, the primer pair being a pair of primers for specifically amplifying the COX1 gene or containing the COX1 gene fragment, and the nucleotide sequence of the COX1 gene being SEQ ID No.1. The present invention utilizes multiple strains of heat laelaps of honey bees separated from domestic popular bee colonies for DNA amplification and sequence analysis comparison, screens the conservative nucleic acid sequence as the target, and develops a primer-probe composition for detecting the heat laelaps of honey bees and a real-time fluorescence PCR detection method. The primer-probe composition and the detection method of the present invention have high sensitivity, a minimum detection limit of 2.0 copies / μL, and good specificity, and have no cross reaction with other bee mites, and can be used for the detection of the heat laelaps of honey bees and the identification of the heat laelaps of honey bees from other bee mites, as well as the quarantine of imported and exported bees and bee products.

The invention provides a composition, a kit and a method for detecting African swine fever virus, and relates to the technical field of gene detection. The primer group and the probe provided by the invention are used for detecting the African swine fever virus, the probe is a ZIPNA probe, the ZIPNA probe comprises a 5 '-terminal fluorophore, a 3'-terminal quenching group and a spermine group, and the ZIPNA probe can provide higher detection specificity because non-specific binding and background signals can be more effectively reduced. The positive ions coupled with the ZIPNA probe can neutralize negative charges of target nucleic acid and reduce electrostatic repulsion, and meanwhile, the ZIPNA probe coupled with a certain number of positive ion groups can reach a preset Tm value. The ZIPNA probe can effectively replace a small groove conjugate and locked nucleic acid, and can be used in various application fields based on nucleic acid detection. The ZIPNA probe has the advantages of being high in detection fluorescence increment, high in detection specificity, good in repeatability, high in sensitivity and the like, and practical application is facilitated.

The invention discloses an enzyme-free fluorescence immunoadsorption method based on DNA three-way junction auxiliary signal amplification, and belongs to the technical field of molecular biological detection. According to the invention, gold nanoparticles are taken as a carrier, an AuNPs-DNA-antibody detection probe is synthesized, an antibody is used for capturing a target object, DNA is used for amplifying a signal, the amplified signal is formed by utilizing a DNA three-way junction structure, and the signal is output through combination of generation of a G-quadruplex and a fluorescent dye. The detection on the staphylococcus aureus enterotoxin B is realized. According to the invention, high-flux, high-specificity and high-sensitivity detection of SEB can be realized. The detection limit is 7.21 ng / L, and the linear range is 0.01-10 ng / mL. The cross reaction rate to staphylococcus aureus enterotoxin A is 34.78%, and the cross reaction rates to bovine serum albumin, hemoglobin, aflatoxin B1, chloramphenicol, oxytetracycline and the like are all lower than 10%.

The invention discloses a tacrolimus personalized medication guiding gene CYP3A5 * 3 polymorphic site detection kit and a use method thereof, the kit utilizes an MGB probe and the energy difference of different polymorphic bases to directly judge the polymorphism of the gene through an amplification curve, and calculation and sequencing analysis are not needed. The kit comprises a pair of primers and a probe which are used for amplifying CYP3A5 * 3 polymorphic sites, the kit further comprises positive samples of three polymorphisms of CYP3A5 * 3CC, CT and TT. The CYP3A5 * 3 typing related sites can be rapidly detected, the sensitivity, specificity and accuracy are superior to those of a first-generation sequencing and double-probe calculation method, rapid and accurate information is provided for individualized drug administration of clinical transplantation patients, and rejection and other side effects after drug administration are avoided.

The invention belongs to the technical field of gene detection, and provides a primer and probe combination and a kit for MAT gene detection of morchella esculenta. The primer and probe combination comprises an MAT1-1-1L / MAT1-1-1R primer, a probe P1-1-1, an MAT1-2-1L / MAT1-2-1R primer, and a probe P1-2-1. The kit comprises the combination of the primer and the probe, # imgabs0 # Univertal Probe qPCR (Quantitative Polymerase Chain Reaction) Master Mix, and Water Nuclease-Free. The invention further discloses a kit for detecting whether the kit is used for detecting whether the kit is used for detecting whether the kit is used for detecting whether the kit is used for detecting whether the kit is used for detecting. Compared with the prior art, the kit has the characteristics of high sensitivity, high specificity and high stability, the procedure is simple, the detection time is short, the result is stable, clear, reliable and easy to break, and the detection efficiency of the MAT gene is improved. The method can be used in the aspects of morchella strain breeding, strain quality detection, cultivation application and the like.

The invention discloses AS-PCR primers and a method for detecting drug resistance of peronophythora litchii to azoxystrobin. An AS-PCR primer capable of being used for detecting whether peronophythora litchii has drug resistance to azoxystrobin or not is obtained by comparing differential sequences of a peronophythora litchii strain capable of resisting azoxystrobin and a peronophythora litchii strain sensitive to azoxystrobin, and a method for detecting the drug resistance of peronophythora litchii to azoxystrobin is constructed based on the AS-PCR primer. The detection specificity is good, and the sensitivity is high. The AS-PCR primer and the detection method are used for amplifying a to-be-detected strain, and if a single band with the consistent size can be obtained, the detected strain is anti-azoxystrobin peronophythora litchii. Compared with a traditional detection method, the detection method disclosed by the invention is short in time consumption, simple, convenient and feasible, is beneficial to quickly monitoring early epidemic of peronophythora litchii anti-azoxystrobin groups, and provides scientific guidance for scientific prevention and control of peronophythora litchii.

The present invention provides a progesterone detection kit, preparation and application method, including three-component solid reagents; the three-component solid reagents include R1 reagent, R2 reagent and R3 reagent; the R1 reagent is a progesterone antibody coated with latex microspheres containing fluorescent and dye substances; the R2 reagent is a progesterone peptide modified with a water-soluble biotin derivative; the R3 reagent is streptavidin coated with latex microspheres containing photosensitive substances; the preparation method of the three-component solid reagent is: the R1 reagent, the R2 reagent and the R3 reagent are diluted with a diluent according to a certain concentration and then freeze-dried. The kit does not require a sequential reaction sequence and a cleaning step, nor does it require a dissociating agent, etc., and is simple to operate.

This invention discloses a pyrimethanil hapten, an artificial antigen, and their preparation methods and applications. The structural formula of the pyrimethanil hapten is shown below. The arm introduced in this invention not only possesses an active group but also fully retains the benzene ring and dimethylpyrimidine ring structure of the target analyte, enhancing the recognition site. The pyrimethanil artificial antigen and monoclonal antibody of this invention exhibit high specificity for ELISA detection, with an IC50 rating of [missing information]. 50 The value is 1.97 μg / L, which is better than existing monoclonal antibodies. It can be used in colloidal gold immunochromatography to rapidly and conveniently detect pyrimethanil. The colloidal gold immunochromatographic test strip prepared in this invention has a detection sensitivity of 10 μg / L for pyrimethanil.

The application discloses a method, system and device for detecting Tau protein based on machine learning of surface enhanced Raman spectroscopy, and the method comprises the following steps: S1, constructing a SERS signal probe with minimum initial Raman signal; S2, mixing a sample to be detected with the SERS signal probe, and collecting SERS spectra of the mixed system by using a Raman spectrometer; S3, pre-processing the collected SERS spectra, wherein the pre-processing comprises baseline correction, feature peak identification and minimum-maximum normalization processing in sequence, so as to obtain standardized spectral data; and S4, inputting the standardized spectral data into a machine learning regression model which is trained in advance, and automatically outputting a concentration value of Tau protein in the sample to be detected based on the input spectral characteristics by the machine learning regression model. The application can quantitatively detect phosphorylated Tau protein quickly, accurately and with low detection limit under the premise of keeping high specificity, and has high clinical conversion potential.

This invention discloses chloramphenicol hapten, antigen, antibody, colloidal gold detection device, and their preparation methods and applications. The chloramphenicol hapten prepared by this invention using a total synthesis method retains all potential recognition sites of chloramphenicol to the maximum extent, and in particular, for the first time retains the most critical dichloro structure of chloramphenicol. After introducing a spacer arm, its electron cloud distribution is almost identical to the overall electron cloud distribution of chloramphenicol, improving the immunogenicity of the chloramphenicol antigen. The chloramphenicol antigen and monoclonal antibody prepared by this invention have high specificity for ELISA detection, with an IC50 value of [missing information]. 50 The value was 8.21 pg / mL, the linear detection range was 1.71–39.45 pg / mL, the cross-reactivity to common structural analogs was less than 0.01%, and the sensitivity to chloramphenicol in the sample was 0.1 µg / kg.

The invention belongs to the technical field of biomedical signal processing, and particularly relates to an automatic respiratory rate calculation method for respiratory sound processing of an electronic stethoscope, which comprises the following steps of: S1, acquiring a period of respiratory sound signal with the duration of Ts (Ts is greater than or equal to 15 seconds) and the sampling rate of fs by using the electronic stethoscope; s2, performing short-time Fourier transform on the obtained breath sound signal vector x; s3, calculating the amplitude S of the time-frequency spectrum matrix S; s4, carrying out homomorphic envelope st on the time domain vector # imgabs0 # of the intermediate frequency component; and S5, searching all peak values greater than a preset threshold Eth on the time domain homomorphic envelope st of the intermediate frequency component. By calculating the time domain homomorphic envelope of the intermediate frequency component, main interference components including heart sound can be automatically eliminated, the detection specificity of the breath sound signal component is greatly improved, and then the calculation precision of the breath rate is improved; respiratory cycle detection and respiratory rate calculation can be realized only by searching a peak value meeting a threshold condition on the time domain homomorphic envelope of the intermediate frequency component without any pattern recognition method with relatively high complexity.

The invention discloses a fluorescence analysis and detection method and system based on quantum dots, and belongs to the technical field of analysis and detection. According to the method, functional modified quantum dots are used as fluorescent probes, a fluorescence detection system is constructed through specific recognition reaction, and high-sensitivity and high-specificity detection of a target detection object is realized by combining collection and quantitative analysis of fluorescence signals. The corresponding system comprises an excitation light source module, a sample reaction module, a fluorescence detection module, a data processing module and a control module, all the modules work cooperatively, key parameters in the detection process can be precisely regulated and controlled, and the detection precision and stability are improved. The method solves the problems of poor probe stability, low quantum yield and high detection limit in a traditional fluorescence detection method, has the advantages of high detection speed, high specificity and wide application range, and can be widely applied to the fields of food safety detection, environmental pollutant monitoring, biomedical diagnosis and the like.

The invention belongs to the technical field of microbiological detection. The piriformospora indica PCR (Polymerase Chain Reaction) detection specific primer is characterized in that the nucleotide sequence of the specific primer is as follows: an upstream primer PI-F: 5 '-CGCTGGAGGAATTTGAGAAG-3'; the downstream primer PI-R is 5 '-CCGTCTTCCCGATAATGATG-3', and the downstream primer PI-R is 5 '- The invention is used for solving the technical problems of high false positive and low amplification efficiency caused by low specificity of the existing EF1 alpha gene detection marker. The invention also discloses a fluorescent quantitative PCR detection method for piriformospora indica, which is realized by using the primer.

The present invention belongs to the field of modern agricultural technology, and specifically relates to a kit for detecting Phytophthora palmitoides, a durian root rot disease, and its application. The present invention designs primers and crRNA for the specific sequence of Phytophthora palmitoides, and develops a rapid visualization detection reagent and kit for Phytophthora palmitoides, a durian root rot disease. The reagent or kit can complete the detection of Phytophthora palmitoides, a durian root rot disease, within 30 minutes, has good specificity and no cross-reaction, and the sensitivity reaches 10 fg / μL. And there is no need to open the cover after the RPA amplification is completed to add the CRISPR / Cas12a reaction system, avoiding aerosol contamination caused by opening the cover. The present invention does not require the use of large-scale instruments and equipment, has the characteristics of short time consumption, simple operation, high sensitivity and specificity, and is suitable for rapid visualization detection of clinical samples and on-site.

The present invention provides an indirect ELISA kit for detecting Tembusu virus antibodies and the application of Tembusu virus E protein domain III, belonging to the field of biological detection technology. The application of Tembusu virus E protein domain III in the preparation of a reagent or kit for detecting Tembusu virus antibodies. The present invention also provides an indirect ELISA kit for detecting Tembusu virus antibodies, comprising a detection plate coated with Tembusu virus E protein domain III and an enzyme-labeled secondary antibody. The kit has good detection specificity and provides technical support for epidemiological surveys, immune effect evaluation, and epidemic prevention and control of Tembusu virus.

The present invention belongs to the technical field of microbial detection, and particularly relates to crustaceans Amazon spp. Microsporidia detection primers and probes and their applications. Amazon spp., provides a conserved nucleic acid region for the SSU rRNA gene Amazon spp. detection. The detection sensitivity of the fluorescent quantitative PCR using the primers and probes of the present invention is 4.58 copies / reaction, which has high sensitivity and strong specificity. The detection primers and detection method of isothermal amplification provided by the present invention can complete the on-site rapid diagnosis of samples under simple conditions, and has Amazon spp. Development and application prospects of detection kits.

This invention relates to immunological detection, particularly to the field of immunological diagnosis of tumors. Specifically, this invention provides a method for diagnosing nasopharyngeal carcinoma based on anti-EBV antibody levels, and a kit for using said method. This invention also provides a polypeptide encoded by the EBV BNLF2b gene for the above-mentioned diagnosis and its use in the diagnosis of nasopharyngeal carcinoma.

The application relates to the field of tumor biotechnology and discloses a long-chain non-coding RNA molecular marker combination and application thereof. The long-chain non-coding RNA molecular marker combination comprises seven molecules, including ENSG00000233397, BARX1-DT, LSAMP-AS1, HOXB-AS4, MNX1-AS1, LINC01385 and AL513318.2; the seven long-chain non-coding RNAs are significantly highly expressed in laryngeal squamous cell carcinoma tissues, each molecule can promote the proliferation, migration and invasion ability of laryngeal squamous cell carcinoma cells, and is positively correlated with the poor prognosis of laryngeal squamous cell carcinoma. The long-chain non-coding RNA molecular marker combination is applied to the preparation of a laryngeal squamous cell carcinoma auxiliary prognosis evaluation kit and a preparation for treating laryngeal squamous cell carcinoma; the laryngeal squamous cell carcinoma auxiliary prognosis evaluation kit adopts real-time fluorescent quantitative PCR to detect the expression amount of the seven long-chain non-coding RNAs, and combines the seven expression amount results to conveniently and efficiently judge the disease condition and prognosis evaluation of a laryngeal cancer patient; and the preparation for treating laryngeal squamous cell carcinoma comprises at least one or seven of the seven long-chain non-coding specific interference RNAs.

The invention relates to a low-altitude water area biochemical toxicity monitoring system and method. The monitoring system comprises an unmanned aerial vehicle platform and a ground monitoring terminal. The unmanned aerial vehicle platform comprises an unmanned aerial vehicle, a controllable folding and unfolding mechanism and a detection unit, and the controllable folding and unfolding mechanism is arranged at the abdomen of the unmanned aerial vehicle; the winding and unwinding mechanism is connected with the detection unit through a winding and unwinding cable, the detection unit is controlled to ascend and descend through the winding and unwinding mechanism, and then the underwater depth of the detection unit is controlled; a signal processing and wireless transmitting unit is arranged in the unmanned aerial vehicle, the ground monitoring terminal is provided with a wireless receiving unit, and the ground monitoring terminal receives wireless signals of the unmanned aerial vehicle, analyzes the wireless signals and displays monitoring results. The optical fiber sensor probe of the modified specific biochemical recognition membrane is integrated with the unmanned aerial vehicle platform, high-precision detection capability is delivered to a target water area by utilizing the maneuverability of the unmanned aerial vehicle, and in-situ and real-time monitoring of low-altitude water area biochemical toxicity is realized.

The invention provides an activatable signal amplification detection technology based on fluorescent dye tyramine and application of the activatable signal amplification detection technology in nucleocapsid protein detection. The problems of insufficient detection sensitivity, poor specificity, loud background noise and the like in a traditional tyramine signal amplification technology are solved by adopting a dual amplification detection technology (combining with an aptamer or an antibody to improve specificity and utilizing tyramine to realize signal amplification) capable of activating tyramine signal amplification and ROS response; and the detection difficulty caused by the own characteristics of the nucleocapsid protein is avoided. According to the technology, the sensitivity, specificity and accuracy of detection are remarkably improved, different virus nucleocapsid proteins can be effectively distinguished, background noise is reduced, a reliable method is provided for SARS-CoV-2 nucleocapsid protein detection, meanwhile, a basis is provided for researching the influence of different structures on the detection effect, and the technology has wide application prospects in the fields of clinical diagnosis and scientific research.

The present invention discloses a peripheral blood TCR marker for predicting the prognosis and efficacy of traditional Chinese medicine for granulomatous mastitis, and its application, detection reagent and kit, belonging to the field of biomedical technology. The amino acid sequence of the peripheral blood TCR marker of the present invention is selected from the group consisting of all amino acid sequences shown in SEQ ID NO.1~100. The peripheral blood TCR marker provided by the present invention can be used for auxiliary diagnosis of the prognosis risk of traditional Chinese medicine for granulomatous mastitis, with good detection specificity, high sensitivity and accuracy, so as to be used for the research and development of related products (including reagents, kits, test strips or instrument platforms, etc.), and the above-mentioned peripheral blood TCR marker can also be used as a target for screening related drugs for the prevention and treatment of granulomatous mastitis.

This invention relates to a double-antibody sandwich ELISA kit for detecting serum PTGS1 protein, comprising: a PTGS1 capture antibody, an unlabeled PTGS1 detection antibody, an enzyme-labeled IgG secondary antibody, a solid-phase carrier, an enzyme substrate, a blocking buffer, a washing buffer, and a stop solution; the IgG secondary antibody specifically binds to the PTGS1 detection antibody; the PTGS1 capture antibody is a rabbit monoclonal antibody that specifically binds to the linear epitope A of human PTGS1 protein, the amino acid sequence of which is SEQ ID NO:1, corresponding to amino acids 271-282 of human PTGS1 protein; the PTGS1 detection antibody is a mouse monoclonal antibody that specifically binds to the B epitope of human PTGS1 protein; the amino acid sequences of the B epitope at the A epitope do not overlap and do not constitute steric hindrance. This kit avoids the conserved homologous region between PTGS1 and its homologous protein PTGS2, ensuring the accuracy of the capture antibody recognition, and uses an indirect detection mode with enzyme-labeled goat anti-mouse IgG secondary antibody to achieve signal cascade amplification, significantly improving detection sensitivity.